Jose Roberto Arruda Leme - Academia.edu (original) (raw)

Papers by Jose Roberto Arruda Leme

Revista Portuguesa de Ciências do Desporto, 2005

Brasil RESUMO O objetivo do presente estudo foi verificar os efeitos do exercício físico agudo e ... more Brasil RESUMO O objetivo do presente estudo foi verificar os efeitos do exercício físico agudo e crônico sobre o eixo hipotálamo-pituitáriaadrenal de ratos administrados com dexametasona. Ratos Wistar jovens foram divididos em quatro grupos: controle sedentário (CS), controle treinado (CT), dexametasona sedentário (DxS) e dexametasona treinado (DxT). O protocolo de treinamento consistiu de natação 1 hora/dia, 5 dias/semana, durante 10 semanas, suportando uma sobrecarga relativa a 5% do seu peso corporal. A dexametasona foi administrada 5 dias/semana (2µg/dia diluída em 150µl de NaCl-0,9%). Antes do sacrifício os ratos receberam insulina subcutânea para o cálculo da remoção máxima de glicose. No final do período experimental amostras de sangue foram obtidas para determinação da glicose e hormônio adrenocorticotrófico (ACTH) séricos. Amostras do músculo gastrocnêmio, da glândula adrenal e tecido adiposo epididimal foram utilizadas para determinação do peso, teor de glicogênio e ácido ascórbico, respectivamente. Nossos resultados indicam que a exposição crônica a dexametasona está associada com diminuição da sensitividade à insulina. O tratamento com dexametasona diminuiu a secreção de ACTH em resposta ao exercício agudo, mostrando diferença no funcionamento do eixo CRH-ACTH-adrenal entre os grupos estudados. Em conclusão, o exercício pode preponderar sobre o feedback negativo da dexametasona na ativação do eixo hipotálamo-pituitária-adrenal.

Cientifica (Sao Paulo), 2005

Gladiolus is a traditional ornamental crop in Brazil, as a cut flower for the domestic market, an... more Gladiolus is a traditional ornamental crop in Brazil, as a cut flower for the domestic market, and more recently, the corm production as an important product for export. The Instituto Agronomico develops research on this culture since the 60´s. In the 70’s it was released a purple striped variety named ‘Itapetininga’ whose commercial value does not persist nowadays. By the end of the 80’s, the breeding program restarted with two main goals: selection of new varieties tolerant or resistant to rust (Uromyces transversalis) and the search for new products, which means, varieties with different colors, forms, and designs. In this paper, two new released varieties are described: ‘IAC Carmim’ and ‘IAC Paranapanema’. Both are hybrids selected among the plants cultivated at Capao Bonito Experimental Station, a unit of the Instituto Agronomico. They are very rustic and vigorous plants. The bulbs have been distributed to the growers mainly from the Southwest region of Sao Paulo State. Twenty-five other pre-selected hybrids are under field multiplication. In the coming years, it will be possible to release new varieties, obtained from controlled pollination, which promise to be very good selections viewing the replacement of the old ones. Additional keywords: Gladiolus X grandiflorus Hort.; Gladiolus aff. dalenii; variety; selection; bulbous plant; corm.

Rev Inst Med Trop Sao Paulo, Apr 1, 1985

Rev Bras Ortop, Feb 1, 1986

verdadeiro; gerado, não criado, consubstancial ao Pai, por Ele todas as coisas foram feitas; e po... more verdadeiro; gerado, não criado, consubstancial ao Pai, por Ele todas as coisas foram feitas; e por nós, homens, e para nossa Salvação, desceu do Céu e se encarnou, pelo Espírito Santo, no seio da Santíssima Virgem Maria, e se fez homem; também por nós foi crucificado sob Pôncio Pilatos, padeceu e foi sepultado; ressuscitou ao terceiro dia, conforme as Escrituras, e subiu ao Céu, onde está sentado à direita do Pai, e de novo há de vir, em Sua glória, para julgar os vivos e os mortos, e o Seu Reino não terá fim; Ao Divino Espírito Santo, Senhor que dá a vida, e procede do Pai e do Filho, e com o Pai e o Filho é adorado e glorificado, Ele que falou pelos Santos Profetas. Desejando imensamente "fazer tudo para a glória de Deus" (I Cor 10,31), a Ele dedico todo o meu trabalho. 5 AGRADECIMENTOS Em primeiro lugar e acima de tudo, agradeço a Deus por todas as bênçãos que Ele derramou sobre minha vida e meu trabalho, sem as quais todo e qualquer êxito seria impossível, pois "se o Senhor não edificar a casa, em vão trabalham os que a constroem; se o Senhor não guardar a cidade, debalde vigiam as sentinelas" (Salmo 126). Agradeço também a todos os Santos e Anjos do Céu por sua preciosa intercessão junto ao trono do Senhor, de modo especial a Nossa Senhora do Carmo,

The aim of this study was to characterize and to describe the game system and the actions (pass, ... more The aim of this study was to characterize and to describe the game system and the actions (pass, dribble, shooting, disarmament and conduct) of professional futsal team, seeing the strategies and the performance area of each player during the games. The sample was 6 teams from different futsal championships professionals. All actions with ball, made by the teams' players, were examined and identified in a virtual field by software Skout®. The field region where the athletes played were identified in two-dimensional coordinate and represented by the main axes. The results showed that the teams using a lot of passes between players and constants handling (a dynamic system). It was clear that the handling with ball possession most used by athletes in professional indoor soccer team is in diagonal direction, both for the direction of the central field and for the lateral direction. The teams adopted predominantly the 3-1 and 4-0 game system and the actions the most used were pass and dribble.

The Open Clinical Chemistry Journal, 2008

Diabetes mellitus is a metabolic disease associated with multiple organ damage, dysfunction and f... more Diabetes mellitus is a metabolic disease associated with multiple organ damage, dysfunction and failure. Metformin is widely used to treat diabetes, but regular exercise also improves metabolic control in diabetic individuals and has an important role in the management of this disease. In this work, we compared the effects of metformin and physical training in diabetic male Wistar rats. Four groups of rats were used: (n=6 per group): sedentary control (SC), sedentary diabetic (SD), trained diabetic (DT) and metformin diabetic (MD). Diabetes was induced with alloxan (30 mg/kg, b.w.). The physical training protocol consisted of a 1 h swimming session/day, five days/week for eight weeks with a load corresponding to 5% of the body weight. Metformin treatment consisted in 1.4 mg/ml per day, administered in the drinking water. At the end of the experimental period, the rats were sacrificed and blood was collected to measure serum glucose, insulin and albumin. Glycogen was quantified in gastrocnemius muscle, liver and heart, the protein/DNA ratio was mensured in liver and heart triglycerides was also measured in the heart. Diabetes reduced the serum insulin and liver glycogen levels and the protein/DNA ratio, but increased the serum glucose and heart glycogen levels; there were no significant variations in serum albumin levels. Physical training increased the muscle glycogen level. Physical training and metformin were equally effective in reducing the serum glucose concentration and in restoring the hepatic and cardiac glycogen stores and the hepatic protein/DNA ratio in diabetic rats. These results show that chronic exercise was as effective as metformin in improving the metabolic profile of diabetic rats and in preventing diabetes-induced alterations.

Revista Brasileira de Psiquiatria, 2010

Revista do Instituto de Medicina Tropical de São Paulo, 1985

Os Autores utilizaram novo anti-helmíntico, o albendazol, no tratamento de 32 pessoas com estrong... more Os Autores utilizaram novo anti-helmíntico, o albendazol, no tratamento de 32 pessoas com estrongiloidíase. A casuística foi composta por adultos, de ambos os sexos, que receberam, pela via oral, dose cotidiana única de 400 mg, repetida em três oportunidades intervaladas por períodos de 24 horas. O controle da terapêutica sucedeu através de exames das fezes realizados sete, 14 e 21 dias após o término da administração, tendo sido empregado o método de Rugai, Mattos e Brisola. A porcentagem de curas verificada correspondeu a 28,1% e, ao lado da boa tolerância observada, essa constatação demonstrou baixa eficácia do medicamento em apreço no combate à infecção causada pelo Strongyloldes stercoralis, a despeito de méritos comprovados em investigações anteriores e concernentes a outras parasitoses intestinais.

Arquivos de Zoologia, 1973

Motricidade, 2012

Dado que o diabetes mellitus é considerado um problema de saúde pública mundial, o presente estud... more Dado que o diabetes mellitus é considerado um problema de saúde pública mundial, o presente estudo objetivou avaliar a tolerância à glicose oral (TTGo) e concentrações de insulina pancreática (CIP) de ratos diabéticos tipo 1 submetidos a um protocolo de treinamento físico individualizado. Foram utilizados 40 ratos Wistar adultos, onde metade foi induzida ao diabetes por meio de injeção endovenosa de aloxana (32 mg/kg), divididos em quatro grupos (10 por grupo): Controle Sedentário (CS), Controle Treinado (CT), Diabético Sedentário (DS) e Diabético Treinado (DT). O treinamento físico consistiu de natação, 1 h/dia, 5 dias/semana durante 8 semanas, com sobrecarga equivalente a 90% da transição metabólica aeróbia/anaeróbia determinada no início do experimento. Os ratos dos grupos DS e DT apresentaram redução de peso corporal em relação aos controles, a qual foi menos acentuada no grupo DT. As ingestões hídrica e alimentar aumentaram nos grupos diabéticos em relação aos controles. Durante o TTGo, os valores de área sob a curva glicêmica dos grupos diabéticos foram superiores aos dos controles. O treinamento físico atenuou esta elevação. Os grupos diabéticos apresentaram CIP reduzida quando comparados aos grupos controles. O protocolo de treinamento físico empregado, embora não tenha alterado a CIP, melhorou a homeostase glicêmica e atenuou a perda de peso corporal dos animais diabéticos. Palavras-chave: diabetes mellitus, treinamento físico, insulina pancreática ABSTRACT Since diabetes mellitus is considered a world public health problem, the present study aimed to evaluate oral glucose tolerance (TTGo) and pancreatic insulin concentration (PIC) of type I diabetic rats subjected to an individualized exercise training protocol. A total of 40 adult Wistar rats were used, half of which induced to diabetes by alloxan (32 mg/kg) endovenous injection, and divided into four groups (10 per group): Sedentary Control (SC), Trained Control (TC), Sedentary Diabetic (SD) and Trained Diabetic (TD). The physical training consisted of swimming, 1 h/day, 5 days/week during 8 weeks, supporting overload equivalent to 90% of the individual anaerobic/aerobic metabolic transition determined at the beginning of the experiment. The rats of SD and TD groups presented body weight reduction in relation to controls, which was less accentuated in TD group. Water and food ingestion increased in the diabetic groups in relation to controls. The areas under the serum glucose curve during the GTTo of diabetic groups were higher than the controls. Physical training attenuated this elevation. The diabetic groups showed reduced PIC when compared to both control groups. The physical training protocol employed improved glucose homeostasis and attenuated the body weight loss of diabetic animals but did not alter pancreatic insulin concentration.

Indian journal of experimental biology, 2008

Insulin is an important modulator of growth and metabolic function in the central nervous system.... more Insulin is an important modulator of growth and metabolic function in the central nervous system. The aim of this study was to investigate the influence of swimming physical training (at 32 degrees +/- 1 degree C, 1 hr/day, 5 days/week, with an overload equivalent to 5% of the body weight, for 4 weeks) on brain insulin concentrations in alloxan induced type 1 diabetic rats. Training attenuated hyperglycemia but had no effect on insulinemia in diabetic rats. Hematocrit and blood albumin values remained without changes. Brain insulin did not change in diabetic rats. However, physical training increased the concentration in both control and diabetic rats. It is concluded that in the present experimental conditions, diabetes had no influence on brain insulin, however moderate physical training increased the hormone in both control and diabetic animals.

Pituitary, 2009

The present study investigated the effects of moderate physical training on some of the parameter... more The present study investigated the effects of moderate physical training on some of the parameters in the GH-IGF axis in experimental diabetic rats. Male Wistar rats were allocated into the following groups: sedentary control, trained control, sedentary diabetic, trained diabetic. Diabetes was induced by alloxan (32 mg/kg, b.w. iv). The physical training protocol consisted of 1 h swimming session/day, 5 days/week for 8 weeks supporting a load corresponding to 90% of maximal lactate steady state. After the experimental period, blood was collected to measure serum glucose, insulin, triglycerides, albumin, insulin-like growth factors-I (IGF-I), and growth hormone (GH). Pituitary gland was removed for GH quantification. Diabetes increased blood glucose and triglycerides and decreased insulin, IGF-I, serum and pituitary GH. Physical training decreased glucose and triglycerides, and also counteracted the reduction of serum IGF-I in diabetic rats. In conclusion, physical training recovered...

Journal of diabetes, 2009

Animal models appear well-suited for studies into the role of exercise in the prevention of non-i... more Animal models appear well-suited for studies into the role of exercise in the prevention of non-insulin-dependent diabetes mellitus (NIDDM). The aim of the present study was to analyze glucose homeostasis and blood lactate during an exercise swimming test in rats treated with alloxan during the neonatal period and/or fed a high calorie diet from weaning onwards. Rats were injected with alloxan (200 mg/kg, i.p.) or vehicle (citrate buffer) at 6 days of age. After weaning, rats were divided into four groups and fed either a balanced diet or a high-caloric diet as follows: C, control group (vehicle + normal diet); A, alloxan-treated rats fed the normal diet; H, vehicle-treated rats fed the high-caloric diet; and HA, alloxan-treated rats fed the high-caloric diet. Fasting serum glucose levels were higher in groups A and AH compared with the control group. The Homeostatic Model Assessment index varied in the groups as follows: H>A>HA = C. There were no differences in free fatty aci...

Islets

To evaluate the effect of acute exercise and exercise training at the anaerobic threshold (AT) in... more To evaluate the effect of acute exercise and exercise training at the anaerobic threshold (AT) intensity on aerobic conditioning and insulin secretion by pancreatic islets, adult male Wistar rats were submitted to the lactate minimum test (LMT) for AT determination. Half of the animals were submitted to swimming exercise training (trained), 1 h/day, 5 days/week during 8 weeks, with an overload equivalent to the AT. The other half was kept sedentary (sedentary). At the end of the experimental period, the rats were submitted to an oral glucose tolerance test and to another LMT. Then, the animals were sacrificed at rest or immediately after 20 minutes of swimming exercise at the AT intensity for pancreatic islets isolation. At the end of the experiment mean workload (% bw) at AT was higher and blood lactate concentration (mmol/L) was lower in the trained than in the control group. Rats trained at the AT intensity showed no alteration in the areas under blood glucose and insulin during ...

Revista Brasileira de Medicina do Esporte, 2013

Introdução: A prática de exercícios de intensidade moderada pode reduzir o risco de infecções e m... more Introdução: A prática de exercícios de intensidade moderada pode reduzir o risco de infecções e melhorar os aspectos metabólicos do indivíduo. Objetivo: Investigar os efeitos do treinamento físico aeróbio sobre os aspectos metabólicos, ósseo e sistema imunológico. Métodos: Vinte ratos machos, recém-desmamados, foram distribuídos em dois grupos experimentais: grupo sedentário (GS) e grupo treinado (GT). O GT foi submetido a um protocolo de natação, durante seis semanas consecutivas. Ao final do período experimental, foi realizada a contagem total e diferencial de leucócitos e hematócrito. Após o sacrifício, foram analisados: glicose, proteínas totais, triglicérides, colesterol; amostras do fígado e músculo para a determinação dos teores de glicogênio; e tíbia para determinação do comprimento e área óssea. Os dados foram analisados pela análise de variância ANOVA one-way e o nível de significância estabelecido foi p<0,05. Resultados: O hematócrito (%) analisado apresentou diferença significativa, com maior valor para o GT (54,63 ± 1,41) que para o GS (49,5 ± 1,65). A contagem total de leucócitos não apresentou diferença significativa, assim como também não houve diferença na contagem diferencial. O colesterol total apresentou relevante diminuição no GT (GT = 68,27 ± 13,71 mg/dL; GS = 94,44 ± 28,09); os níveis de proteínas totais também apresentaram importante redução (GT = 7,3 ± 0,40 g/dL; GS = 7,74 ± 0,36 g/dL); os níveis de glicose e triglicérides não apresentaram diferenças significativas. Já o comprimento ósseo apresentou diferença significativa, com o comprimento do tibial do GT (40 ± 0,14* mm) sendo menor que o GC (42,10 ± 0,12 mm). A área tibial demonstrou menor valor para o GT (1,53 ± 0,12 cm²) que para o GS (1,67 ± 0,18 cm²), entretanto, a diferença não foi estatisticamente significante. Conclusão: O treinamento físico aeróbio é capaz de produzir algumas modificações fisiológicas peculiares em ratos jovens.

Hippocampus, 2009

The present study investigated the role of swimming training on cerebral metabolism and hippocamp... more The present study investigated the role of swimming training on cerebral metabolism and hippocampus concentrations of insulin and IGF-1 in diabetic rats. Wistar rats were divided in sedentary control (SC), trained control (TC), sedentary diabetic (SD), and trained diabetic (TD). Diabetes was induced by Alloxan (35 mg kg 21 b.w.). Training program consisted in swimming 5 days/week, 1 h/day, 8 weeks, supporting a load corresponding to 90% of maximal lactate steady state (MLSS). For MLSS determination, rats were submitted to three sessions of 25-min supporting loads of 4, 5, or 6% of body wt, with intervals of 1 week. Blood samples were collected every 5 min for lactate determination. An acute exercise test (25 min to 90% of MLSS) was done in 7th week to confirm the efficacy of training. All dependent variables were analyzed by one-way analysis of variance (ANOVA) and a significance level of P < 0.05 was used for all comparisons. The Bonferroni test was used for post hoc comparisons. At the end of the training period, rats were sacrificed and sample blood was collected for determinations of serum glucose, insulin, GH, and IGF-1. Samples of gastrocnemius muscle and liver were removed to evaluate glycogen content. Hippocampus was extracted to determinate glycogen, insulin, and IGF-1 contents. Diabetes decreased serum GH, IGF-1, and liver glycogen stores in SD. Diabetes also increased hippocampus glycogen and reduced hippocampus IGF-1 content. Physical training recovered liver and hippocampus glycogen stores and promoted increases in serum IGF-1 in TD group. Physical training restored hippocampus IGF-1 content in diabetic group. It was concluded that in diabetic rats, physical training induces important metabolic and hormonal alterations that are associated with an improvement in glucose homeostasis and with an increased activity in the systemic and hippocampus IGF-1 peptide.

Medicine & Science in Sports & Exercise, 2009