Joseph Ashidi - Academia.edu (original) (raw)

Papers by Joseph Ashidi

The FASEB Journal, Apr 1, 2019

In traditional medicine, Laportea aestuans (LA)is used in treatment of several ailments including... more In traditional medicine, Laportea aestuans (LA)is used in treatment of several ailments including pain and inflammatory conditions. This study evaluates the analgesic and anti-inflammatory potential of the ethanol extract of the leaves of LA using hotâ€“plate induced analgesia and carrageenanâ€“induced acute inflammatory model in Albino Wistar rats (120g-160g). The analgesic activity was assessed by oral administration of the extract doses; 50, 100 and 150 mg/kg bwt to separate groups of rats, 100 mg/kg bwt of ibuprofen (positive control) and 10 mL/kg bwt of distilled water (negative control).0.1mLof 1% carrageenan suspended in distilled water was injected intra-peritonially into the sub-plantar region of the right-hind paw of the rats to induce inflammation. Similar doses were given to test its anti-inflammatory activity but 10 mg/kg of diclofenac sodium was given as the positive control. The results obtained showed significant (p<0.05) dose -dependent difference among the group...

Annals of Health Research

Background: While the reproduction-enhancing property of Mucuna pruriens (MP) seed has been widel... more Background: While the reproduction-enhancing property of Mucuna pruriens (MP) seed has been widely studied in males, little is known about this property in females despite the rate at which the seed is consumed by both sexes worldwide. Objective: To determine the effect of MP seed powder in dietary inclusion on ovarian and uterine functions of adult female albino rats. Methods: The rats were randomised into four groups. Group 1 (Control) was given standard rat chow (15g of feed/rat/day only) while groups 2, 3 and 4 were fed diets supplemented with MP seed powder at 0.75 g, 1.5g and 2.25g/day, respectively, for 12 weeks. Serum levels of oestradiol, follicle stimulating hormone, luteinising hormone, ovarian Δ5, 3β- hydroxysteroid dehydrogenase (Δ5, 3β-HSD) and 17 β hydroxysteroid dehydrogenase (17β-HSD) activities, ovarian and uterine peroxidase and tissue cytoarchitectural structures were used as diagnostic markers of reproductive function. Results: Significant increases in the serum...

Pharmaceutical Biology, 2005

Seven methanol extracts of seven plants from seven plant families were screened for antimalarial ... more Seven methanol extracts of seven plants from seven plant families were screened for antimalarial properties. The plants were identified and selected from Gboko and Kastina-Ala local government areas in the Tivland ethnobotany in the Middle Belt Zone of Nigeria. Methanol plant extracts were evaluated for in vitro antimalarial properties using the lactate dehydrogenase technique, with a multiresistant strain of Plasmodium falciparum K1. Quantification of activity was by estimation of the concentration of extracts that inhibited 50% growth of parasite (IC 50) in mg=ml. Of the seven plants screened, Erythrina senegalensis DC (Leguminosae), Pericopsis elata Harms (Papilionaceae), and Bridelia micrantha Benth (Fabaceae) had IC 50 values of 99.7, 124.8, and 158.7 mg=ml, respectively. Nauclea latifolia SM (Rubiaceae) extract exhibited the least activity in the assay with an IC 50 value of 478.9 mg=ml.

THE EGYPTIAN JOURNAL OF EXPERIMENTAL BIOLOGY (Botany)

Environmental science and pollution research international, 2018

The increase in the frequency of exposure to gasoline fumes and the growing incidence of infertil... more The increase in the frequency of exposure to gasoline fumes and the growing incidence of infertility among humans has been a major concern and subject of discussion over the years in Nigeria. We therefore present the reproductive effect of gasoline fumes on inhalation exposure in 40 male albino rats. The rats were randomized into five experimental treatments (T) with eight rats per treatment. T1 (control) was exposed to distilled water while T2, T3, T4, and T5 were exposed to gasoline fumes in exposure chambers for 1, 3, 5, and 9 h daily respectively for 12 weeks. Serum level of testosterone, follicle stimulating hormone (FSH), luteinizing hormone (LH), prolactin, oxidative stress markers in the testicular tissue, epididymal sperm health assessment, and testicular histopathology of the rats were used as a diagnostic marker of reproductive dysfunction. Significant (p < 0.05) alterations in the levels of all the reproductive hormones and oxidative stress markers assayed were observ...

Beni-Suef University Journal of Basic and Applied Sciences

Experimental and Toxicologic Pathology, 2017

The incidence of infertility in human is on the increase and the use of Roundup herbicide and pre... more The incidence of infertility in human is on the increase and the use of Roundup herbicide and presence of its residues in foodstuff is a major concern. This study therefore aim to assess the effect of Roundup on the reproductive capacity of 32 adult male albino rats randomized into 4 groups of 8 rats per group orally exposed to Roundup at 3.6mg/kg body weight(bw), 50.4mg/kgbw and 248.4mg/kgbw of glyphosate concentrations for 12 weeks while the control group was given distilled water. Serum level of reproductive hormone (testosterone, luteinizing hormone (LH), follicle stimulating hormone (FSH) and prolactin), oxidative stress indices in the testicular tissue, epididymal sperm morphology assessment and testicular histopathology of the rats were used as a diagnostic marker of reproductive dysfunction. Significant (p&amp;amp;amp;amp;amp;lt;0.05) alterations in the level of all the reproductive hormones and oxidative stress markers assayed were observed in rats exposed to Roundup. Significant reductions (p&amp;amp;amp;amp;amp;lt;0.05) in sperm count, percentage motility and significant (p&amp;amp;amp;amp;amp;lt;0.05) increased in abnormal sperm cells were observed in the exposed rats. Histopathologically, severe degenerative testicular architectural lesions were seen in the Roundup exposed rats. Roundup may interfere with spermatogenesis and impair fertility in male gonad.

Objective: In order to evaluate the ethnomedical uses of plants as remedy for malaria, in Southwe... more Objective: In order to evaluate the ethnomedical uses of plants as remedy for malaria, in Southwestern Nigeria, six crude methanol extracts obtained from five plant species, identified and selected from ethnomedicine, were studied for in vitro anti-plasmodial activity and cytotoxicity. Method: The antiplasmodial properties were evaluated in vitro, using the lactate dehydrogenase assay against Plasmodium falciparum (multi drug resistant K1) and the cytotoxicity activities were assessed using KB nasopharyngeal cell line. Thereafter, three of the crude extracts were fractionated and subjected to activity studies. Results: Methanol extracts of three of the plant extracts; Cassia siamea stem bark, Tithonia diversifolia leaf and Cajanus cajan leaf, were found to display intrinsic anti-plasmodial properties with IC 50 values of 24.9 µg/ ml, 52.9 µg/ml and 53.5 µg/ml, respectively. Fractionation of three crude extracts using organic solvents led to acquisition of twelve fractions, of which the ethyl acetate fraction of C. cajan displayed the highest activity with IC 50 value of 15.6 µg/ml. The other active fractions were the aqueous methanol of C. siamea bark and ethyl acetate fractions of Gossypium arboreum, both displaying anti-plasmodial activities, with an IC 50 value of 31.3 µg/ml. The crude methanol extract of T. diversifolia was found to be most toxic in the cytotoxicity assay, with an ED 50 value of 3.6 µg/ml and least selective to the malaria parasites. Conclusions: The ethnomedicine of southwestern Nigeria could provide leads for the discovery of antimalarial drugs.

Journal of Ethnopharmacology, 2003

The ethnographic study was conducted in two communities in Oyo State in Southwestern Nigeria. The... more The ethnographic study was conducted in two communities in Oyo State in Southwestern Nigeria. The study sites consisted of a rural and an urban local government area located in the tropical rain forest zone of Nigeria. The study was designed to obtain information on febrile illnesses and herbal remedies for treatment with the aim of identifying potential antimalarial drugs. The study revealed that fever is a general term for describing illnesses associated with elevated body temperature. The indigenous Yoruba ethnic population has categorized fever based on symptoms and causes. The present communication is the result of focus group discussion and semi-structured questionnaire administered to traditional healers, herb sellers, elders and mothers. This was on types of fevers, symptoms and causes of febrile illnesses. The investigation also included use of traditional herbs in the prevention and treatment of the illnesses in the two communities. A total of 514 respondents were interviewed. This was made up of 266 (51.8%) from Atiba local government area (LGA), an urban centre while 248 (48.2%) respondents were interviewed from Itesiwaju LGA, a rural community. The LGAs are located in Oyo State of Nigeria. The respondents proffered 12 types of febrile illnesses in a multiple response answering system in Yoruba language. The most common ones (direct translation into English) were: yellow fever (39.1%), typhoid (34.8%), ordinary (28.8%), rainy season (20.8%) and headache (10.5%) fevers, respectively. Perceived causes of each of the febrile illnesses included stress, mosquito bites, unclean water, rains and over exposure to the sun. Methods of fever prevention were mainly with the use of herbal decoctions, powdered herbs, orthodox medications and maintenance of proper hygiene. Of a total of 112 different herbal remedies used in the treatment of the febrile illnesses compiled from the study, 25 recipes are presented. Recipes consisted of 2-7 ingredients. Oral decoctions (84%), oral powders (63%), use as soaps and creams (40%) in a multiple response system, were the most prevalent routes of administration of prepared herbs used in the treatment of the fevers. Boiling in water or alcohol was the most common method used in the preparation of the remedies. The four most frequently mentioned (multiple response system) plants in the Southwest ethnobotany for fevers were Azadirachta indica (87.5%), Mangifera indica (75.0%), Morinda lucida (68.8%) and Citrus medica (68.8%).

NIgerian Journal of Neuroscience

Antlion is used in traditional medicine by natives of the southern part of Nigeria particularly t... more Antlion is used in traditional medicine by natives of the southern part of Nigeria particularly the Yorubas for memory enhancement. The progress made so far in the use of this organism as a memory booster lead to investigating the acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) (the key enzymes in the pathogenesis of Alzheimer's disease) inhibitory activities of methanolic and phosphate buffered saline (PBS) extracts. The activities of these enzymes were investigated using Ellman’s method. The kinetics of the inhibition patterns was also studied using eserine as the standard inhibitor. The concentration of the extract required for 50% inhibition (IC50) of the AChE was 49.00 ± 1.20 and 271.40 ± 0.10 µg/mL, for the PBS and methanolic extracts respectively, compared to Eserine with IC50 of 2.25 x 10-2 ± 0.15 x 10-2 µg/mL. Similarly, the IC50 for the BuChE was 66.30 ± 0.40 and 216.70 ± 1.10 µg/mL respectively, for the methanol and PBS extracts, compared to Eserine with...

Scientia Africana

There has been an increasing rate of cannabis consumption globally, especially among the youths. ... more There has been an increasing rate of cannabis consumption globally, especially among the youths. This study therefore evaluated the neurological behaviours and some brain marker hormones and enzymes of cannabis administered rats. Twenty six albino rats were divided into four groups based on oral cannabis administration (control, Cannabis sativa, Cannabis indica and the combination of the two). At the end of seven days, open field test was conducted on the rats. Also, brain neuro-chemicals, activities of antioxidant enzymes and lipid peroxidation were evaluated using spectrophotometry. The results of the Open-Field Test showed an appreciable increase in the level of ambulation (line crossing), grooming, urination and stretched attend posture in the rats administered with Cannabis indica, Cannabis sativa and the combination when compared with the control. Norepinephrine was significantly lower (p < 0.05) in the rat groups administered with the combination of Cannabis indica and Can...

Heliyon

The occurrence of conflicting reports on the improvement of male reproductive function among huma... more The occurrence of conflicting reports on the improvement of male reproductive function among humans fed diet containing the seed of Mucuna pruriens (MP) have raised a major concern in Nigeria. We assessed the effect of MP seed powder on the reproductive function in 32 adult male albino rats randomized to 4 groups of 8 rats each for 8 weeks. Group 1 (control) was given standard rat chow while groups 2, 3 and 4 were fed diets containing MP seed powder at 0.75 g, 1.5 g and 2.25 g respectively. Serum level of follicle stimulating hormone, testosterone, luteinizing hormone, oxidative stress markers in the testis, epididymal sperm quality and cytoarchitectural structure of the testis were monitored using standard methods. Significant improvements (p < 0.05) in the serum levels of all the hormones, testicular antioxidant defence, epididymal sperm quality without testicular degenerative changes were observed in group 2 compared to other groups. Oxidative stress, severe degenerative architectural lesions in the testis as well as significant reductions (p < 0.05) in epididymal sperm count, percentage motility and significant (p < 0.05) increased in abnormal sperm cells were observed in group 4. MP seed enhances reproductive function in male rats only at a dose level of 0.75g. A dose value higher than 0.75 g may be toxic to the male reproductive system.

African journal of medicine and medical sciences, 2004

In the course of evaluating the contribution of phytomedicine to possible drug discovery of antim... more In the course of evaluating the contribution of phytomedicine to possible drug discovery of antimalarial drugs, an ethnomedical survey of specialized children traditional clinics was done. In the observational multi center study, efficacy of eight different herbal remedies, each consisting of 3-8 ingredients and administered by herbalists were investigated in clients enrolled in the six traditional clinics in Oyo (urban center) and Otu (rural center) of Oyo State, Nigeria. The clients, aged between six months and fifteen years with clinical symptoms of malaria were enrolled in the clinics of the herbalists, as their usual practice. Oral informed consents were obtained from their parents or guardians. Microscopic diagnosis of malaria infection was used to evaluate parasitaemia and validate efficacy of herbal remedies. Results of the analysis showed that, of the 163 clients of the herbalists, only 62 (30 from Oyo, 32 from Otu) had microscopically confirmed P. falciparum infection. Onl...

African journal of medicine and medical sciences

This report is on the antimicrobial potential of Bridelia micrantha, Alchornea cordifolia and Boe... more This report is on the antimicrobial potential of Bridelia micrantha, Alchornea cordifolia and Boerhavia diffusa sourced from traditional healers through an ethnobotanical survey of antiinfective plants in Egbado South in Ogun State, Nigeria. Extracts of B. micrantha and A. cordifolia exhibited significant inhibitory activity against the pathogenic organisms. In some cases, the antibacterial activity was comparable to those of ampicillin and gentamycin. However, only the leaf of A. cordifolia showed reasonable antifungal activity when compared with Trosyd. The study shows that there is justification for the use of these medicinal plants in traditional medicine.

Journal of Basic Microbiology, 2005

Indilinga: African Journal of Indigenous Knowledge Systems, 2009