Juan Cabello - Academia.edu (original) (raw)
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Lahore College For Women University Lahore Pakistan
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Papers by Juan Cabello
Algal Research, 2020
This work explores the influence of controlled acidification on lipid accumulation in S. obtusius... more This work explores the influence of controlled acidification on lipid accumulation in S. obtusiusculus AT-UAM under N-deplete conditions. The protocol included determination of the acidification procedure (CO 2 or HCl) and the application chronology in flat panel photobioreactors, operated under controlled conditions. The highest lipid content and productivity of 60% g lipids g DCW −1 and 85 mg L −1 d −1 , respectively, were obtained when controlled acidification to pH of 5.5 was applied after 80 h of operation under N-depletion. The strategy was also applied successfully in a larger scale using different configurations such as bubble-column photobioreactors and raceway ponds, operated outdoors, yielding a maximum lipid content of 45% g lipids g DCW −1 in both systems. Globally, the lipid content increased almost twice the value obtained without acidification in indoor and outdoor conditions. The analysis revealed that no major changes in fatty acid profile occurred, suggesting a high specificity of the microalga toward lipid production; however, significant changes in the ultrastructure of the subcellular components and the cell wall were observed.
The Science of the total environment, Jan 15, 2017
The extensive microalgae diversity offers considerable versatility for a wide range of biotechnol... more The extensive microalgae diversity offers considerable versatility for a wide range of biotechnological applications in environmental and production processes. Microalgal cultivation is based on CO2 fixation via photosynthesis and, consequently, it is necessary to evaluate, in a short time and reliable way, the effect of the CO2 gas concentration on the consumption rate and establish the tolerance range of different strains and the amount of inorganic carbon that can be incorporated into biomass in order to establish the potential for industrial scale application. Dynamic experiments allow calculating the short-term microalgal photosynthetic activity of strains in photobioreactors. In this paper, the effect of step-changes in CO2 concentration fed to a 20L bubble column photobioreactor on the CO2 consumption rate of Scenedesmus obtusiusculus was evaluated at different operation times. The highest apparent CO2 consumption rate (336μmolm(-2)s(-1) and 5.6% of CO2) was 6530mgCO2gb(-1)d(...
Bioresource Technology, 2015
Photosynthetic activity assays allowed obtaining optimal culture conditions. The optimal pH, T an... more Photosynthetic activity assays allowed obtaining optimal culture conditions. The optimal pH, T and I were higher for N-replete than for N-deplete conditions. Biomass productivity was 0.97 g L À1 d À1 at optimal conditions in flat panel reactor. Lower activation energy was required for lipid accumulation than for growth. High photosynthetic efficiency was reached under nitrogen starvation.
Algal Research, 2020
This work explores the influence of controlled acidification on lipid accumulation in S. obtusius... more This work explores the influence of controlled acidification on lipid accumulation in S. obtusiusculus AT-UAM under N-deplete conditions. The protocol included determination of the acidification procedure (CO 2 or HCl) and the application chronology in flat panel photobioreactors, operated under controlled conditions. The highest lipid content and productivity of 60% g lipids g DCW −1 and 85 mg L −1 d −1 , respectively, were obtained when controlled acidification to pH of 5.5 was applied after 80 h of operation under N-depletion. The strategy was also applied successfully in a larger scale using different configurations such as bubble-column photobioreactors and raceway ponds, operated outdoors, yielding a maximum lipid content of 45% g lipids g DCW −1 in both systems. Globally, the lipid content increased almost twice the value obtained without acidification in indoor and outdoor conditions. The analysis revealed that no major changes in fatty acid profile occurred, suggesting a high specificity of the microalga toward lipid production; however, significant changes in the ultrastructure of the subcellular components and the cell wall were observed.
The Science of the total environment, Jan 15, 2017
The extensive microalgae diversity offers considerable versatility for a wide range of biotechnol... more The extensive microalgae diversity offers considerable versatility for a wide range of biotechnological applications in environmental and production processes. Microalgal cultivation is based on CO2 fixation via photosynthesis and, consequently, it is necessary to evaluate, in a short time and reliable way, the effect of the CO2 gas concentration on the consumption rate and establish the tolerance range of different strains and the amount of inorganic carbon that can be incorporated into biomass in order to establish the potential for industrial scale application. Dynamic experiments allow calculating the short-term microalgal photosynthetic activity of strains in photobioreactors. In this paper, the effect of step-changes in CO2 concentration fed to a 20L bubble column photobioreactor on the CO2 consumption rate of Scenedesmus obtusiusculus was evaluated at different operation times. The highest apparent CO2 consumption rate (336μmolm(-2)s(-1) and 5.6% of CO2) was 6530mgCO2gb(-1)d(...
Bioresource Technology, 2015
Photosynthetic activity assays allowed obtaining optimal culture conditions. The optimal pH, T an... more Photosynthetic activity assays allowed obtaining optimal culture conditions. The optimal pH, T and I were higher for N-replete than for N-deplete conditions. Biomass productivity was 0.97 g L À1 d À1 at optimal conditions in flat panel reactor. Lower activation energy was required for lipid accumulation than for growth. High photosynthetic efficiency was reached under nitrogen starvation.