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Papers by KARINY APARECIDA JARDIM RUBIO

Research, Society and Development, Jun 25, 2022

bactéria e pelas condições fisiológicas e anatômicas da gestante, tornando-se, dessa forma, uma a... more bactéria e pelas condições fisiológicas e anatômicas da gestante, tornando-se, dessa forma, uma ameaça de propagação de infecções por cepas multirresistentes. Palavras-chave: Infecção estaflocócica; Gestação; mecA; MRS.

Revista de Ciência Veterinária e Saúde Pública, May 26, 2017

Semina-ciencias Agrarias, Oct 26, 2016

Sheep breeding has been important in agribusiness, transforming the Brazilian productive scenario... more Sheep breeding has been important in agribusiness, transforming the Brazilian productive scenario. However, it is still deficient due to the damages caused by infectious diseases. Leptospirosis is a severe disease with global distribution, caused by bacteria from the Leptospira genre affecting both humans and animals. The general infection is unapparent, or its clinical signs, when present, are similar to other infections. Brucellosis is an infectious disease caused by bacteria from the Brucella genre responsible for reproductive disorders in animals, especially ruminants. The purpose of this paper was to seroepidemiological study of Leptospira spp. and Brucella ovis in sheep and dogs from nonmechanized rural properties from the northwestern region in the state of Paraná, Brazil. In order to detect anti-Leptospira antibodies, microscopic agglutination (MAT) was performed. For anti-Brucella antibodies, the agar gel immunodiffusion assay (AGID) was performed. From the total 542 samples from sheep sera analyzed, 11.25% were considered reagent to Leptospira spp. and 18.26% to Brucella ovis. From the 36 dog samples, 25% were reagent to MAT and AGID. From the 32 properties analyzed, 75% were considered positive for leptospirosis and 56.25% for brucellosis. Antibodies against the most probable serovars were Hardjo (34.42%) and Butembo (44.44%) in sheep and dogs, respectively, and the variable exchange of animals among properties was associated to leptospiric infection (p=0.028) in sheep. Leptospirosis and brucellosis are present in the sheep herd and dogs in the rural properties studied, and such result is a warning of the zoonotic importance and the need to establish sanitary programs directed to these animal species.

Animals, Mar 24, 2023

This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY

Semina-ciencias Agrarias, Apr 26, 2016

Bacterial resistance is a primary public health concern worldwide. Within this context, pets and ... more Bacterial resistance is a primary public health concern worldwide. Within this context, pets and breeding animals act as reservoirs for multidrug-resistant bacteria (MR), such as those producing extended spectrum beta-lactamases (ESBL) and those presenting plasmid-mediated quinolone resistance (PMQR). The aim of this study was to detect the presence of ESBL and PMQR in members of the Enterobacteriaceae family, isolated from healthy sheep and dogs from non-intense farming rural properties in the Umuarama region of Paraná, Brazil. A total of 81 oral and rectal swabs from dogs and sheep from 11 small rural properties were analyzed. These swabs were inoculated in tubes containing brain heart infusion broth (BHI), and the resulting cultures were inoculated on MacConkey agar (MAC) supplemented with 10 μg/mL cefotaxime for the selection of ESBL producers. The cells were also plated on MAC supplemented with 50 μg/mL nalidixic acid for selecting quinolone-resistant enterobacteria. The bacterial isolates were subjected to biochemical identification tests, antibiograms, double-disk synergic tests, and polymerase chain reaction analysis for resistance-inducing genes (bla ESBL , qnr, and genes encoding efflux pump and acetylases). Four (5.00%) bacterial isolates (3 Escherichia coli and 1 Morganella morganii) resistant to cephalosporins and/or quinolones were identified; of these, three (75%) isolates were from sheep and one (25%) from a dog. These findings indicate the presence of MR bacteria in the normal microbiota of the animals studied. Animals colonized with such bacteria can

Arquivo Brasileiro de Medicina Veterinária e Zootecnia

Concilium

This study aimed to screen phytase-producing basidiomycetes by submerged cultivation (SmC) with p... more This study aimed to screen phytase-producing basidiomycetes by submerged cultivation (SmC) with particulate agro-industrial byproducts and evaluate the enzyme's thermal and pH stability. Five among eight fungal species produced high phytase activity (> 300 U mL-1) after seven days in SmC. Ganoderma sp., the best phytase producer (1130 U mL-1), was grown in a liquid medium with particulate agro-industrial byproducts (coffee husks, citric pulp, sugarcane bagasse, and soybean hulls). Soybean hulls increased phytase activity (16%) and productivity (2.3-fold). The residual phytase activity was determined after 120 min incubation in different pH (pH 3, 5, 7, and 9) and temperatures (30, 50, and 70 oC). Phytase activity remained stable at pH ranging from 3 to 9, with 100% activity retention after 120 min at pH 7. Furthermore, Phytase retained more than 60% activity when incubated for 120 min from 30 oC to 70 oC. Our findings support future studies using Ganoderma sp. and particulate...

Research, Society and Development

Os Staphylococcus spp. têm sido descritos como integrantes rotineiros da microbiota do ser humano... more Os Staphylococcus spp. têm sido descritos como integrantes rotineiros da microbiota do ser humano em superfície de regiões mais úmidas do corpo, enquanto algumas espécies e subespécies apresentam predileção pela região urogenital. Também são considerados oportunistas e frequentemente tem sido causa de infeção em pacientes com comprometimento do sistema imunológico ou em uso de dispositivos invasivos em pacientes hospitalizados. No entanto, nos últimos anos, houve uma disseminação dessas cepas pela comunidade, o que possivelmente pode estar relacionado a pressão seletiva pelo uso inadequado dos antibióticos. As infecções causadas por Staphylococcus spp. meticilina resistente tem causado sérios problemas dentro e fora do ambiente hospitalar. Dentro da comunidade causa colonização de pessoas aumentando a chance de óbito em todos os sexos e faixa etária e em gestantes têm sido consideradas importantes fontes de microrganismos patógenos pelos fatores de virulência apresentados por essa b...

Semina: Ciências Agrárias, 2016