Leptospirosis and brucellosis seroepidemiology in sheep and dogs from non-mechanized rural properties in the northwestern region in the state of Paraná (original) (raw)
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Brazilian Journal of Veterinary Research and Animal Science, 2020
The present study aimed to estimate the prevalence of antibodies against Brucella ovis-epididymitis, smooth-Brucella,leptospirosis, toxoplasmosis and Maedi-visna in sheep slaughtered in Minas Gerais, Brazil and to study their simultaneousoccurrence, including caseous lymphadenitis, at sheep and flock levels. The study was conducted at a sheep slaughterhousewith Federal Inspection Service. Sera from 594 animals from 21 flocks were collected, in 2007. The agar gel immunodiffusion(AGID) was employed to detect anti-B. ovis and anti-Maedi Visna antibodies, whereas Rose Bengal (RB) and the2-mercaptoethanol test (2ME) were used to test anti-smooth Brucella antibodies. For the detection of anti-Leptospiraantibodies, sera were examined by microscopic agglutination test (MAT), while for the detection of IgG antibodies toToxoplasma gondii ELISA was used. Prevalence of antibodies against smooth Brucella, B. ovis-epididimitis, Leptospiraspp., toxoplasmosis and Maedi-Visna found in sheep from Min...
Cross-Sectional Study of Leptospira spp. and Brucela abortus in Goat Herds from Paraná State, Brazil
Acta Scientiae Veterinariae
Background: Leptospirosis and brucellosis are zoonosis worldwide distributed that have great economic importance, especially in goats. This study aimed to carry out a cross-sectional study to determine the prevalence of anti-Leptospira spp. and anti-Brucella abortus antibodies and to determine epidemiological variables associated to infection by these ethiological agents in goats.Materials, Methods & Results: Serum samples were collected from 1055 goats of 95 properties distributed in 18 regional centers of Paraná State from April to August of 2010. Collected samples were submitted to microscopic agglutination test (MAT) for the detection of anti-Leptospira spp. antibodies with 22 reference serovars: Australis, Bratislava, Autumnalis, Butembo, Castellonis, Bataviae, Canicola, Fortbragg, Whitcombi, Cynopteri, Grippotyphosa, Hebdomadis, Copenhageni, Icterohaemorrhagiae, Panama, Pomona, Pyrogenes, Hardjo, Wolffi, Shermani, Tarassovi and Londrina 1 (L1). The samples were also submitted ...
Semina: Ciências Agrárias, 2015
The objective of this study was to determine the frequency of animals that tested positive for brucellosis and leptospirosis and the risk factors for these diseases in the State of Paraíba. A total of 136 buffaloes from 14 herds were examined. For brucellosis, we used the buffered acidified plate antigen (BAPA) test as screening method and 2-mercaptoethanol as confirmatory test. For leptospirosis, we conducted a microscopic agglutination test (MAT), with a cutoff point of 1:100. Of the animals examined, two [1.5%; 95% CI = 0.4%-5.2%] were positive for brucellosis, and 38 (27.9%; 95% CI = 21.1%-36.0%) were positive for leptospirosis. The brucellosis-positive animals were from two (14.3%) herds, while nine (64.3%) herds had leptospirosis-seropositive animals. The more frequent Leptospira spp. serotypes were Bratislava, Pomona, and Canicola. We concluded that leptospirosis was widespread in buffaloes in the state of Paraíba and suggested that breeding alongside horses and pigs might be an important factor in the spread of leptospirosis-positive animals. The presence of brucellosispositive animals indicated the possibility of negative-impacting measures on disease control in bovines, and it is therefore recommended that greater attention be given to these animals for brucellosis control.
Veterinary Science Development, 2014
Leptospirosis and brucellosis are common zoonosis that affect many species of mammals mostly causing economical losses. Further, very important fact is huge danger for human and animal health around the world. The purpose of the study is to determine the prevalence of Brucella spp. and Leptospira spp. using multiplex polymerase chain reaction (mPCR) method, in blood samples collected from cattle, sheep and goats. In this study, a total number of 250 blood samples (5 cc of blood with ethilen diamin tetra asetic acid) were collected randomly from 100 cattle, 80 sheep and 70 goats located on 6 herds in Chaharmahal Va Bakhtiari and Esfahan provinces, Iran. After DNA extraction and setting of mPCR for Brucella spp. and Leptospira spp. mPCR products were screened. The DNA of these microorganisms was detected by multiplex PCR from 31 and 21 out of 100 cattle, respectively. Four of 70 goat's blood samples from goat breeding farms were positive for Leptospira spp. and 11 were positive for Brucella spp. Out of 80 sheep blood samples 23 were positive for Brucella spp. and 14 for Leptospira spp. The results of the present study show ruminant as an important reservoir for transmission of these zoonotic diseases to humans in Iran. mPCR has the ability to concurrently detect both Brucella and Leptospira species from blood samples of ruminants. The convenience and the possibility of detection of both bacteria at a time, strongly support the use of this mPCR for routine diagnostics.
Veterinary Science Development, 2014
Leptospirosis and brucellosis are common zoonosis that affect many species of mammals mostly causing economical losses. Further, very important fact is huge danger for human and animal health around the world. The purpose of the study is to determine the prevalence of Brucella spp. and Leptospira spp. using mPCR method, in blood samples collected from cattle, sheep and goats. In this study, a total number of 250 blood samples (5cc of blood with ethilen diamin tetra asetic acid) were collected randomly from 100 cattle, 80 sheep and 70 goats located on 6 herds in Chaharmahal Va Bakhtiari and Esfahan provinces (Iran). After DNA extraction and setting of mPCR for Brucella spp. and Leptospira spp. mPCR products were screened. The DNA of these microorganisms was detected by multiplex PCR from 31 and 21 out of 100 cattle, respectively. Four of 70 goat’s blood samples from goat breeding farms were positive for Leptospira spp. and 11 were positive for Brucella spp. Out of 80 sheep blood samples 23 were positive for Brucella spp. and 14 for Leptospira spp. The results of the present study show ruminant as an important reservoir for transmission of these zoonotic diseases to humans in Iran. mPCR has the ability to concurrently detect both Brucella and Leptospira species from blood samples of ruminants. The convenience and the possibility of detection of both bacteria at a time, strongly support the use of this mPCR for routine diagnostics
2017
Brucellosis is an infectious disease that affects domestic animals, producing heavily losses through abortions, and infertility in rams.The flock epidemiology situation in the north Patagonic area of Argentina (La Pampa) to the present disease is unknown; consequently the objective was to determine the prevalence of the disease. Out of 10.000 sheep, there were sampled 1.800 animals according to the application of the minimum sample size for estimating a rate with specified degree of precision in the Departments of Mara-co, Chapaleufu, Realico, Rancul, Conhelo and Quemu-Quemu representing an area of 22.072 km2, in the frame of reintroduction of sheep production in the province. Independently of the number of sheep per farm, 20- 30% of the flock was sampled, that represent 15% (270) rams and 85% (1.530) ewes. After collecting serum from jugular blood, the samples were analysed either for the presence of antibodies against smooth strain through Buffer Plate Agglutination Test (BPA); or...
Veterinary Journal, 2007
In spite of the large number of goats found in several developing tropical countries, milk production remains unsatisfactory. The occurrence of infectious diseases, such as leptospirosis, brucellosis and caprine arthritis-encephalitis (CAE) may in part be responsible for sub-optimal production. In this study, 1000 serum samples were tested for leptospirosis, 953 for brucellosis and 562 for CAE. All tested flocks presented at least one seroreactive animal for leptospirosis and for CAE. Reactivity to leptospirosis was 11.1%, and serovar hardjo was the most frequently found. Anti-B. abortus agglutinins were found in 0.5% of the samples presented and 14.1% were seroreactive to CAE. Leptospirosis was considered to represent the major infectious problem in the studied goat flocks. The occurrence of infectious diseases in the tested flocks may represent an important factor contributing to the decreased productivity of the animals. These findings may be similar to those observed in other developing countries and require further study to define the relationship between seropositivity and reduced production.
Seroprevalence of Brucella abortus and Leptospira hardjo in cattle
Veterinary World, 2015
The aim of the study was to assess the seroprevalence of B. abortus and Leptospira hardjo in the cattle population of Bihar. Materials and Methods: Randomly selected 450 cattle from nine districts of Bihar were serologically screened for antibodies against L. hardjo and B. abortus. DAS-ELISA for leptospira and AB-ELISA for brucella were carried out. Based on the results prevalence in each district and the state are reported herewith. Results: In this study, it was found that the seroprevalence of L. hardjo was 9.11% and that of B. abortus was 12.2% in Bihar. Indigenous cattle were found to be less susceptible to leptospirosis and brucellosis even though they accounted for 83.11% of the study population. Conclusion: Although there was no acute disease, antibodies detected against L. hardjo and B. abortus in the cattle population indicated the presence of chronic and subclinical infection, which could challenge the fertility of the animals.
Serological study of brucellosis in Argentine Creole sheep
Revista Argentina de microbiologia, 2018
Ovine cattle was introduced into America during the Spanish conquest with the second journey of Columbus to the Antilles and was disseminated throughout the region. In 1587, sheep were introduced into Argentina, later developing into the "Creole" breed. We selected 486 animals from different Argentine provinces with the aim of determining the serological status of brucellosis caused by Brucella melitensis and Brucella ovis. For the detection of antibodies against smooth Brucella spp., the Rose Bengal test (RBT) was performed as screening test while the serum agglutination test (SAT) and 2 mercapto-ethanol (2ME) were run as a confirmatory technique. Moreover, for the detection of antibodies against rough Brucella spp., we used the rapid slide agglutination test (RSAT) for screening and an indirect ELISA (IELISA) as confirmatory assay. This study showed that the total positive percentage of brucellosis due to B. ovis was 2.9%. Excluding the animals mixed with the Suffolk bre...
Seroepidemiology of sheep brucellosis in the microregion of Feira de Santana, BA, Brazil
Brazilian Journal of Veterinary Research and Animal Science, 2013
The aim of the present study was to perform a seroepidemiological survey of Brucella ovis in ovine flocks in the microregion of Feira de Santana, State of Bahia (BA), Brazil. Ten municipalities with the largest sheep flocks were selected for this survey: Antonio Cardoso, Feira de Santana, Ipecaetá, Ipirá, Itatim, Pintadas, Rafael Jambeiro, Santa Teresinha, Santo Estévão and Serra Preta. The sample size was established on the basis of three parameters: significance level (99%), sampling error (5%), and estimated prevalence (50%). The total sample was divided proportionally to the sheep population found in the respective municipalities. The flocks examined in each municipality were randomly selected. The animals were older than six months and were distributed among 49 properties in the municipalities. Samples of blood from 793 male and female sheep were analyzed. During visitations, an epidemiological questionnaire was applied for collection of information and analysis of possible risk factors. All sera samples were analyzed by agar gel immunodiffusion (AGID) and the antigen was a mixture of soluble proteins and lipopolysaccharides from B. ovis (strain Reo 198). Seropositive animals (6.94%, 55/793) to B. ovis were detected. However, significant statistical difference (p>0.05) was not found for age and sex. Risk factors that might be associated with cases of seropositive animals for the variables analyzed were not found. In 61.22% (30/49) of the examined farms at least one seropositive animal was detected. Only two of the ten municipalities above had no seropositive animal.