Rachid Kaidi - Academia.edu (original) (raw)

Papers by Rachid Kaidi

Research paper thumbnail of Follicular fluid and serum biochemical and hormonal profiles of normal and cystic dromedary camel breeds

Veterinary World

Background and Aim: Ovarian cysts (OC) in female dromedary camels have been described as problema... more Background and Aim: Ovarian cysts (OC) in female dromedary camels have been described as problematic because they can cause infertility. This study aimed to compare the hormone concentrations and biochemical contents present in serum and follicular fluid of normal and cystic she-dromedaries of the two most common Algerian camel breeds (Sahraoui and Targui) to gain a better understanding of biological differences that may yield insights into preventing or treating this ovarian abnormality. Materials and Methods: At an abattoir in southeastern Algeria, 100 pairs of the same females' ovaries and blood samples were taken immediately after the slaughter of clinically healthy, non-pregnant females (8–15 years old) over two consecutive breeding seasons (November 2017–April 2018 and November 2018–April 2019). The concentrations of glucose, cholesterol, protein, urea, creatinine, triglyceride, gamma-glutamyl transferase, alanine aminotransferase, and aspartate aminotransferase were deter...

Research paper thumbnail of Pathological Findings in Cattle Slaughtered in Northeastern Algeria and Associated Risk Factors

Veterinary Sciences

Meat is a food of animal origin, which can be contaminated by infectious, parasitic and other non... more Meat is a food of animal origin, which can be contaminated by infectious, parasitic and other non-infectious agents responsible for diseases, which threaten the health of consumers. This still poses a public health problem in Algeria and in many countries. In order to assess the epidemiological situation of certain diseases in the Taher region in Jijel and to determine the influence of certain variation factors and to estimate the risk on public health, a study was extended over a period of 14 months on a total of 1756 cattle slaughtered at the Taher slaughterhouse. The results showed that 609 cattle (34.68%) showed lesions. The highest rate of pathological findings was observed on the liver (37.27%) followed by the lungs (30.21%). The lowest rate was recorded on the digestive system (0.33%) followed by the kidneys (1.14%). In addition, the liver and lungs were more contaminated with hydatid cyst compared to other organs (20.69%, 19.05%, respectively). Our data showed that the disea...

Research paper thumbnail of Description of Dystocia in Ewes of the Ksar El Boukhari Region (Algeria)

Agricultura, 2019

The region of Ksar el Boukhari is an important site for sheep farming in the high plateaus of cen... more The region of Ksar el Boukhari is an important site for sheep farming in the high plateaus of central Algeria, the main product of which is the lamb. However, the prevalence of neonatal mortality has been scarcely studied. A longitudinal study involving ten veterinary facilities in the region provided us with a detailed description on 171 cases of dystocia, including bad presentations (39%), cervical atony/non-dilation of the cervix (19%), (true) cervical atresia (10%), and uterine torsion (8%). Twenty-nine percent of the cases of dystocia were presented at the veterinary facilities on the same day. Manual relief was provided in 62% of the cases. The remaining cases were relieved surgically.

Research paper thumbnail of Probiotic effect on reserve mobilization in late stage pregnancy in goats

Veterinarska stanica, 2021

This study was carried out to evaluate a probiotic effect on the mobilisation of body reserves in... more This study was carried out to evaluate a probiotic effect on the mobilisation of body reserves in goats towards the end of gestation by measuring ketone body production in blood and calculating the body condition score (BCS). Ketone body production was monitored by measuring the betahydroxybutyrate BHB levels in the blood using a portable kit (PRECISION XTRA Blood ketone test). The results revealed that the use of the probiotic SYMBIOVEBA® (association of Saccharomyces cervicae and Lactobacillus) did not decrease the formation of blood BHB in goats, and therefore did not decrease mobilization of the body reserves of goats in late pregnancy.

Research paper thumbnail of Effect of Sprouted Trigonella foenum graecum L. Incorporation into the Diet on Milk Production of Rabbit Does and Growth of Young Rabbits in the Northeast of Algeria

Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Veterinary Medicine, 2021

The aim of this study was to assess the effect of sprouted fenugreek incorporation into the diet ... more The aim of this study was to assess the effect of sprouted fenugreek incorporation into the diet of rabbits on their dairy and growth performances. Data from 24 lactations performed by 8 Californian rabbit does were studied. They were divided into 2 homogeneous groups, a control (batch C) having received an ordinary ration and an experimental (batch E) having received the same ration supplemented with sprouted fenugreek. Growth rates of suckling pups were also recorded. Then, Growth performance of 152 young rabbits’ issue from these females was monitored. They were divided into 4 homogeneous groups, one control having received an ordinary diet (batch C) and 3 experimental having received the same diet with substitution of 12%, 15% and 25% (batch E1, E3 and E3) of the concentrate by sprouted fenugreek. The results showed an improvement in the dairy performance of the rabbit does of batch E, with an average peak lactation of 287 g/d vs 236 g/d in batch C (p <0.01). The daily weight...

Research paper thumbnail of Ovarian tumors in cattle: Case reports

Materials and methods Samples were obtained during routine inspection from Holstein cows proposed... more Materials and methods Samples were obtained during routine inspection from Holstein cows proposed to be slaughtered in an abattoir (El-Harrach, Algiers, Algeria). There is no information about the reproductive history and the age of the cows. After macroscopic examination of the ovaries, oviducts, uterus and vagina of each animal, selected tissues of the genital tract were fixed in 10% buffered formalin and embedded in paraffin wax. During the dissection of the ovaries, the liquid contained in ovarian tumors was aspirated and stored separately at −20°C till analysis. Microscopic examination of the fixed tissues was carried out at the pathological anatomy laboratories of the NHSV (National High School of Veterinary, Algiers, Algeria) and of Douira Hospital (Algiers, Algeria). Sections cut at 3μm thickness were stained with Hematoxylin and Eosin (H&E), and evaluated under a light microscope. Concentration of progesterone, estradiol 17 β and testosterone were assessed by Radio Immuno A...

Research paper thumbnail of Prevalence and anatomohistopathologic studies of uterine anomalies in she-camels in southeast Algeria

Elwishy 1989) to 43,9 % (Al-Afaleq et al 2012). The discrepancy in these prevalence rates presuma... more Elwishy 1989) to 43,9 % (Al-Afaleq et al 2012). The discrepancy in these prevalence rates presumably relates to the application of different diagnostic methods, with variable test performance, and the use of different classification systems to define the abnormalities. Comparing to other species, there are many gaps in our understanding of the etiology and pathogenesis, and evolution of this common disorder in camelids. Failure to diagnose early and provide appropriate treatment may result in serious outcomes. Investigation of camel reproductive abnormalities based on abattoir survey of specimens could be effective for providing useful information on prevalence of reproductive disorders and their incidence. In camel, the histopathological criteria of uterine abnormalities are largely understudied. For this reason, our study was carried out to obtain further information on prevalence of the uterine lesions and the histopathological features of uterine specimens of she-camels in Southern Algeria. Materials and methods A total of 740 genital tracts of female camels slaughtered in two abattoirs (El-Oued and Touggourt) were examined in Southeast of Algeria, from February 2011 to June 2013. No information was available on the clinical history of the animals. The reproductive organs of each camel were carefully removed and examined for gross pathological changes. Specimens from uteri showing gross abnormalities were fixed in 10% neutral formalin, processed in paraffin. Sections of 4 μm thickness were cut from formalin-fixed, and stained with hematoxylin and eosin (H&E) to examine the histopathological features such as hemorrhage, infiltration of lymphocytes polymorphonuclear, neutrophil,

Research paper thumbnail of Difference in tick infestations

Veterinarska stanica, 2020

The aim of this study was to identify the species and parasitic indices of ticks. A flock of 57 i... more The aim of this study was to identify the species and parasitic indices of ticks. A flock of 57 individuals (sheep, goats, cattle and dogs) in southern Algeria was studied from March 2019 to February 2020 during monthly visits. A total of 2544 ticks were collected by examining the entire body of the animal. Two tick species were identified: Hyalomma dromedarii (2430 ticks) and Rhipicephalus sanguineus sensu lato (114 ticks). The first species had annual activity and consisted only of adults, while the second species had spring and summer activity and contained both adults and nymphs. At the annual level, the prevalence of infestation, abundance, and intensity were 38.60%, 44.63 and 115.64 ticks per animal, respectively. At the monthly level there were species specific fluctuations, with a peak in activity in September.

Research paper thumbnail of Characterization of ovarian follicular and cystic fluids in cows

Veterinaria, 2018

The study was aimed at evaluating the hormonal, histological and metabolic changes associated wit... more The study was aimed at evaluating the hormonal, histological and metabolic changes associated with ovarian cyst (OC) formation in cows. For this purpose, , the fluid was aspirated from 195 ovarian cysts and 120 ovarian large follicles collected at a local abattoir in order to assess their hormonal concentrations and metabolic changes. Pieces of the cyst wall tissue were subjected to histologic evaluation. Data showed that the cysts’ fluid was characterized by the lower concentrations of glucose, cholesterol, total protein and higher urea levels than those of the large follicles (P 1, whereas the luteal cysts all had E/P ratio < 1. It can be speculated that the abnormal levels of some biochemical and hormonal parameters may lead to follicle dysfunction resulting in cyst formation. Keywords: ovary, cyst, biochemistry, hormone, cow

Research paper thumbnail of Agricultural Academy SEMEN PARAMETERS AND THEIR SEASONAL VARIATIONS OF LOCAL ARBIA BREED BUCKS IN WESTERN ALGERIA

Belhamiti, T.B., A. Ait Amrane, S.M. Hammoudi, S.M.A. Selles, A.R. Benia, E.A. Mammeri and R. Kai... more Belhamiti, T.B., A. Ait Amrane, S.M. Hammoudi, S.M.A. Selles, A.R. Benia, E.A. Mammeri and R. Kaidi, 2018. Semen parameters and their seasonal variations of local Arbia breed bucks in Western Algeria. Bulg. J. Agric. Sci.,

Research paper thumbnail of Ovarian cysts in cattle

Veterinarska stanica, 2021

Ovarian cysts (OC) are among the major factors affecting dairy cattle fertility due to their impa... more Ovarian cysts (OC) are among the major factors affecting dairy cattle fertility due to their impacts on reproductive performance. Extension of the calving-calving interval and the costs of treatmentassociated with this pathology are the main sources of economic losses for the dairy industry. This study involved conducting an epidemiological survey relating to ovarian cysts in cows based on the observations of veterinary practitioners in Algeria. This survey was performed using a questionnaire distributed to 103 practicing veterinarians in different regions of Algeria. According to the responses, OC dominated all ovarian diseases, followed by smooth ovaries and ovarian adhesions. Ovarian tumours were encountered very rarely. In general, veterinarians considered any follicular structure greater than 20 mm in diameter and persisting for at least 10 days to be cystic. The aetiology was multifactorial, and the following were cited: alimentation, high milk production, puerperal pathologie...

Research paper thumbnail of Investigation of the presence of slime production, VanA gene and antiseptic resistance genes in Staphylococci isolated from bovine mastitis in Algeria

Veterinarska stanica, 2020

Staphylococcus strains are frequently as- sociated with clinical and subclinical bovine intra-mam... more Staphylococcus strains are frequently as- sociated with clinical and subclinical bovine intra-mammary infection. The virulence factors of staphylococcus have not been widely studied in Algeria. The objective of this study was to determine the frequency of slime production, VanA gene and antiseptic resistance genes in staphylococci strains isolated from bovine mas- titis in Algeria. The study examined 35 Staphy- lococci strains obtained from the inflammatory secretion of mammary glands of cows with mastitis. Slime production was determined by detecting the icaA and icaD genes using the polymerase chain reaction (PCR) method and Congo red agar (CRA) method. The presence of qacAB and qac C antiseptic resistance genes and the VanA resistance gene in these isolates was investigated by PCR. The results of the current study revealed that of the 35 Staphylo- cocci isolates, 42.85% (15/35) and 17.14% (6/35) of the isolates harboured the slime production gene by analysing icaA and icaD genes,...

Research paper thumbnail of Alternative treatment of bovine mastitis

Veterinarska stanica, 2021

The treatment of mastitis is based mainly on the use of antibiotics. However, in recent times, an... more The treatment of mastitis is based mainly on the use of antibiotics. However, in recent times, an increase in resistance phenomena and the presence of residues in milk and their derivatives has been reported. Few studies have focused on the treatment of mastitis by homeopathy elsewhere or in Algeria. The objective of this current study aimed to clarify in particular the interest of homeopathy in the treatment of mastitis. The study was carried out on two dairy farms on a total of 14 cows located in Laghouat region (southern Algeria). Before the start of the experiment, a tolerance test was performed on two cull cows free from any apparent infection (except mastitis). Local and general reactions were noted at specific times. A total of 31 mammary quarters received 4 intramammary injections of a homeopathic preparation containing several natural products every 12h over 48h. A clinical examination and an analysis of the milk samples on D0, D7 and D14 were performed on all cows and the ...

Research paper thumbnail of Effects of GnRH or hCG on day 11 after artificial insemination in cows luteal activity

Journal of the Hellenic Veterinary Medical Society, Feb 5, 2019

In order to optimize luteal function, human chorionic gonadotrophin (hCG) or gonadotrophin releas... more In order to optimize luteal function, human chorionic gonadotrophin (hCG) or gonadotrophin releasing hormone (GnRH) were used on day 11 after artificial insemination (AI). 33 cows synchronized by the Ovsynch and divided into 3 groups according to the type of treatment: 1) hCG (1500 IU, n=11); 2) GnRH (100 µg, n=11) and 3) control (2 mL of saline, n=11). Blood samples were collected from all animals every 3 days from day 5 to day 23 to determine progesterone concentration. Ultrasonography was used to monitor the luteal surface structures at the time of blood sample collection. An accessory corpus luteum (CL) formed in 63.63% of cows treated with GnRH or hCG, resulting in an increase in the total luteal tissue area compared with the controls. Compared with the controls, the principal CL area was increased by hCG but not by GnRH. Additionally, compared with the control group, hCG-treated cows had increased progesterone concentrations (p<0.0001), while GnRH-treated cows had P4 similar to that of controls cows.

Research paper thumbnail of Development of in-house RIA kit for progesterone in cow’s skim milk

Journal of Immunoassay and Immunochemistry, 2019

A radioimmunoassay (RIA) method for progesterone has been developed. It has been validated for th... more A radioimmunoassay (RIA) method for progesterone has been developed. It has been validated for the determination of progesterone in cow skim milk. The main reagents used in the development work were prepared and characterized inhouse. The assay uses a radioiodinated tracer purified by gel filtration on Sephadex G-25, standards prepared in skim milk and coated tubes with specific antibodies as the solid phase (separation system). The radiochemical purity of the tracer was greater than 95%, the maximum binding using solid phase reached 43% and the nonspecific binding didn't exceed 5%. Series of progesterone standards using milk matrix with concentration ranging from 0 to 40ng/mL were prepared. Detection limit of the assay was 0.13ng/mL and the precision evaluation gives an intra and inter-assay coefficient variations between 4.94% and 12.66%. The recovery obtained with skim milk samples was 84% to 119% and the parallelism test indicated good linearity (R 2 > 0.99). The clinical tests give high correlation coefficient (r = 0.998) between progesterone concentrations of cow's skim milk assayed using developed progesterone RIA kit and commercial progesterone RIA kit.

Research paper thumbnail of Feeding practices of dairy cows in Algeria: Characterization, typology, and impact on milk production and fertility

Journal of Advanced Veterinary and Animal Research, 2019

Objective: To explore feeding strategies and identify eventual errors that could cause poor produ... more Objective: To explore feeding strategies and identify eventual errors that could cause poor production and reproduction performances in dairy farms in the north of Algeria. Materials and Methods: A survey was conducted among 211 farms of different sizes, from 2014 to 2018 to compose a database that is analyzed statistically. Results: The results relative to the nutritive value of the rations showed an average dry matter intake of 15.9 ± 4.74 kg/cow/day, providing 13.2 ± 4.34 UFL (Feed Unit for Lactation) of net energy and 1306 ± 456 gm of protein digested in small intestine (PDI)/cow/day. A high proportion of concentrate intake is observed, with an average of 64.7% ± 17.4% of energy intake and 70.2% ± 16.2% of nitrogen intake. Dairy cows performances were characterized by a low milk production regarding their genetic potential (14.2 ± 4.73 kg of milk/cow/day), a calving interval > to one year (397 ± 20.4 days), though the coverage rates of their nutrient requirements reached 120%. The typology of the dietary rations allowed grouping them into three categories: deficient rations cluster (DR), correct rations cluster, and excessive rations cluster (ER). DR and ER, which are not adjusted to animals' needs, were found in 57.8% of farms. Conclusion: It is obvious that the feeding management in the dairy farms in the North of Algeria is not based upon scientific achievements, contributing to relatively low performances of cows as well as to important financial losses. This finding imposes the necessity to adopt a correct and accurate rationing of animals.

Research paper thumbnail of Retrospective study of the reproductive performance of Barb and Thoroughbred stallions in Algeria

July-2019, 2019

Background and Aim: The Barb horse occupies a prominent place in the history, culture, and equest... more Background and Aim: The Barb horse occupies a prominent place in the history, culture, and equestrian traditions of the Maghreb (Algeria, Morocco, and Tunisia). Although many studies on the breed standard and morphometry have been published, there are no studies on its fertility and reproductive potential. Thus, this work aimed to study the fertility of Barb, Arabian Purebred, and Thoroughbred horses in Algeria. Materials and Methods: A total of 168 stallions and 1202 mares at the Chaouchaoua Stud farm in Tiaret, Algeria, were included in the study. The reproductive performance during 1592 cycles over 10 consecutive mating seasons (2003-2012) was evaluated. Multivariate analysis with logistic regression was used to analyze the reproductive parameters such as the number of cycles operated, number of pregnant mares, pregnancy rate per cycle, seasonal pregnancy rate, and embryonic mortality rate, and to determine the influence of breed, stallion's age, and year of the study on repr...

Research paper thumbnail of Comparative efficacy of two-dimensional mode and color Doppler sonography in predicting gender of the equine fetus

Veterinary World, 2019

Background and Aim: Ultrasonographic fetal sexing is of utmost economic importance for horse bree... more Background and Aim: Ultrasonographic fetal sexing is of utmost economic importance for horse breeders. Relatively, a few studies have been conducted to determine fetal sex in mare using transrectal Doppler ultrasound. This study aimed to compare two sexing techniques, two-dimensional (2D) mode and color Doppler ultrasonography. Materials and Methods: The study was conducted on 39 mares under field conditions. Examinations were performed using the ultrasonic model device (Medison SonoAce Pico, South Korea), equipped with real-time 3-7 MHz convex multifrequency transducer. Fetal sex diagnosis was carried out in two periods of pregnancy, early period (57-80 days of gestation) and late period (80-150 days of gestation). Results: No difference (p=0.4) was observed between the efficiency of the 2D mode and Doppler ultrasound in sex determination with the respective frequency of 74% and 85%. The best time to sex the early fetus was between 57 and 70 days of gestation and between 90 and 120...

Research paper thumbnail of Séroprévalence de la fièvre Q chez les bovins de la région de Bejaïa (Algérie)

Revue d’élevage et de médecine vétérinaire des pays tropicaux, 2017

La fièvre Q est une zoonose bactérienne causée par Coxiella burnetii, une bac­térie intracellulai... more La fièvre Q est une zoonose bactérienne causée par Coxiella burnetii, une bac­térie intracellulaire stricte, de répartition mondiale. Les ruminants domestiques sont le principal réservoir de la bactérie. L’objectif de cette étude a été de déter­miner la séroprévalence de la fièvre Q chez des bovins de la région de Bejaïa, au nord de l’Algérie. Un total de 180 sérums provenant de 50 élevages laitiers a été analysé par la technique Elisa (enzyme-linked immunosorbent assay). Dix-neuf sérums provenant de 11 élevages étaient positifs, soit une prévalence indi­viduelle de 10,6 % (intervalle de confiance [IC] à 95 %, 6,1 % à 15,0 %) et une séroprévalence troupeau de 22 % (IC 95 %, 10,5 % à 33,5 %). Une cor­rélation statistiquement significative a été mise en évidence entre la séropositi­vité et l’existence d’antécédents de rétention placentaire (p = 0,025), de métrites (p < 0,001) ou de retour régulier en chaleurs (p = 0,026) chez les vaches. La fièvre Q circule donc dans les élevages b...

Research paper thumbnail of Variations de l’état corporel d’ovins et systèmes d’élevage dans la région de Chlef, Algérie

Revue d’élevage et de médecine vétérinaire des pays tropicaux, 2017

L’état corporel de lots de brebis mises à la reproduction a été noté dans des exploitations de la... more L’état corporel de lots de brebis mises à la reproduction a été noté dans des exploitations de la région de Chlef en Algérie de février 2012 à avril 2013. L’état corporel et les réserves des brebis ont varié selon, d’une part, la catégorie des femelles concernées (agnelages en septembre-octobre ou en mars-avril) et, d’autre part, l’exploitation. La prise en compte des quantités de fourrage distribuées en complément du pâturage et des réserves corporelles a permis d’établir un diagnostic sur l’alimentation dans chaque situation. L’interaction entre l’état corporel des brebis mises à la lutte et les performances de reproduction a été mise en évidence. En effet, à mesure que la note d’état corporel (NEC) à la mise à la lutte augmentait, la fertilité et la prolificité s’amélioraient. Les brebis dont la NEC à la mise à la lutte a été supérieure ou égale à 3 ont obtenu des taux de fertilité de 100 % et de prolificité de 130 %, contre respectivement 76 % et 100 % pour celles dont la NEC à ...

Research paper thumbnail of Follicular fluid and serum biochemical and hormonal profiles of normal and cystic dromedary camel breeds

Veterinary World

Background and Aim: Ovarian cysts (OC) in female dromedary camels have been described as problema... more Background and Aim: Ovarian cysts (OC) in female dromedary camels have been described as problematic because they can cause infertility. This study aimed to compare the hormone concentrations and biochemical contents present in serum and follicular fluid of normal and cystic she-dromedaries of the two most common Algerian camel breeds (Sahraoui and Targui) to gain a better understanding of biological differences that may yield insights into preventing or treating this ovarian abnormality. Materials and Methods: At an abattoir in southeastern Algeria, 100 pairs of the same females' ovaries and blood samples were taken immediately after the slaughter of clinically healthy, non-pregnant females (8–15 years old) over two consecutive breeding seasons (November 2017–April 2018 and November 2018–April 2019). The concentrations of glucose, cholesterol, protein, urea, creatinine, triglyceride, gamma-glutamyl transferase, alanine aminotransferase, and aspartate aminotransferase were deter...

Research paper thumbnail of Pathological Findings in Cattle Slaughtered in Northeastern Algeria and Associated Risk Factors

Veterinary Sciences

Meat is a food of animal origin, which can be contaminated by infectious, parasitic and other non... more Meat is a food of animal origin, which can be contaminated by infectious, parasitic and other non-infectious agents responsible for diseases, which threaten the health of consumers. This still poses a public health problem in Algeria and in many countries. In order to assess the epidemiological situation of certain diseases in the Taher region in Jijel and to determine the influence of certain variation factors and to estimate the risk on public health, a study was extended over a period of 14 months on a total of 1756 cattle slaughtered at the Taher slaughterhouse. The results showed that 609 cattle (34.68%) showed lesions. The highest rate of pathological findings was observed on the liver (37.27%) followed by the lungs (30.21%). The lowest rate was recorded on the digestive system (0.33%) followed by the kidneys (1.14%). In addition, the liver and lungs were more contaminated with hydatid cyst compared to other organs (20.69%, 19.05%, respectively). Our data showed that the disea...

Research paper thumbnail of Description of Dystocia in Ewes of the Ksar El Boukhari Region (Algeria)

Agricultura, 2019

The region of Ksar el Boukhari is an important site for sheep farming in the high plateaus of cen... more The region of Ksar el Boukhari is an important site for sheep farming in the high plateaus of central Algeria, the main product of which is the lamb. However, the prevalence of neonatal mortality has been scarcely studied. A longitudinal study involving ten veterinary facilities in the region provided us with a detailed description on 171 cases of dystocia, including bad presentations (39%), cervical atony/non-dilation of the cervix (19%), (true) cervical atresia (10%), and uterine torsion (8%). Twenty-nine percent of the cases of dystocia were presented at the veterinary facilities on the same day. Manual relief was provided in 62% of the cases. The remaining cases were relieved surgically.

Research paper thumbnail of Probiotic effect on reserve mobilization in late stage pregnancy in goats

Veterinarska stanica, 2021

This study was carried out to evaluate a probiotic effect on the mobilisation of body reserves in... more This study was carried out to evaluate a probiotic effect on the mobilisation of body reserves in goats towards the end of gestation by measuring ketone body production in blood and calculating the body condition score (BCS). Ketone body production was monitored by measuring the betahydroxybutyrate BHB levels in the blood using a portable kit (PRECISION XTRA Blood ketone test). The results revealed that the use of the probiotic SYMBIOVEBA® (association of Saccharomyces cervicae and Lactobacillus) did not decrease the formation of blood BHB in goats, and therefore did not decrease mobilization of the body reserves of goats in late pregnancy.

Research paper thumbnail of Effect of Sprouted Trigonella foenum graecum L. Incorporation into the Diet on Milk Production of Rabbit Does and Growth of Young Rabbits in the Northeast of Algeria

Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Veterinary Medicine, 2021

The aim of this study was to assess the effect of sprouted fenugreek incorporation into the diet ... more The aim of this study was to assess the effect of sprouted fenugreek incorporation into the diet of rabbits on their dairy and growth performances. Data from 24 lactations performed by 8 Californian rabbit does were studied. They were divided into 2 homogeneous groups, a control (batch C) having received an ordinary ration and an experimental (batch E) having received the same ration supplemented with sprouted fenugreek. Growth rates of suckling pups were also recorded. Then, Growth performance of 152 young rabbits’ issue from these females was monitored. They were divided into 4 homogeneous groups, one control having received an ordinary diet (batch C) and 3 experimental having received the same diet with substitution of 12%, 15% and 25% (batch E1, E3 and E3) of the concentrate by sprouted fenugreek. The results showed an improvement in the dairy performance of the rabbit does of batch E, with an average peak lactation of 287 g/d vs 236 g/d in batch C (p <0.01). The daily weight...

Research paper thumbnail of Ovarian tumors in cattle: Case reports

Materials and methods Samples were obtained during routine inspection from Holstein cows proposed... more Materials and methods Samples were obtained during routine inspection from Holstein cows proposed to be slaughtered in an abattoir (El-Harrach, Algiers, Algeria). There is no information about the reproductive history and the age of the cows. After macroscopic examination of the ovaries, oviducts, uterus and vagina of each animal, selected tissues of the genital tract were fixed in 10% buffered formalin and embedded in paraffin wax. During the dissection of the ovaries, the liquid contained in ovarian tumors was aspirated and stored separately at −20°C till analysis. Microscopic examination of the fixed tissues was carried out at the pathological anatomy laboratories of the NHSV (National High School of Veterinary, Algiers, Algeria) and of Douira Hospital (Algiers, Algeria). Sections cut at 3μm thickness were stained with Hematoxylin and Eosin (H&E), and evaluated under a light microscope. Concentration of progesterone, estradiol 17 β and testosterone were assessed by Radio Immuno A...

Research paper thumbnail of Prevalence and anatomohistopathologic studies of uterine anomalies in she-camels in southeast Algeria

Elwishy 1989) to 43,9 % (Al-Afaleq et al 2012). The discrepancy in these prevalence rates presuma... more Elwishy 1989) to 43,9 % (Al-Afaleq et al 2012). The discrepancy in these prevalence rates presumably relates to the application of different diagnostic methods, with variable test performance, and the use of different classification systems to define the abnormalities. Comparing to other species, there are many gaps in our understanding of the etiology and pathogenesis, and evolution of this common disorder in camelids. Failure to diagnose early and provide appropriate treatment may result in serious outcomes. Investigation of camel reproductive abnormalities based on abattoir survey of specimens could be effective for providing useful information on prevalence of reproductive disorders and their incidence. In camel, the histopathological criteria of uterine abnormalities are largely understudied. For this reason, our study was carried out to obtain further information on prevalence of the uterine lesions and the histopathological features of uterine specimens of she-camels in Southern Algeria. Materials and methods A total of 740 genital tracts of female camels slaughtered in two abattoirs (El-Oued and Touggourt) were examined in Southeast of Algeria, from February 2011 to June 2013. No information was available on the clinical history of the animals. The reproductive organs of each camel were carefully removed and examined for gross pathological changes. Specimens from uteri showing gross abnormalities were fixed in 10% neutral formalin, processed in paraffin. Sections of 4 μm thickness were cut from formalin-fixed, and stained with hematoxylin and eosin (H&E) to examine the histopathological features such as hemorrhage, infiltration of lymphocytes polymorphonuclear, neutrophil,

Research paper thumbnail of Difference in tick infestations

Veterinarska stanica, 2020

The aim of this study was to identify the species and parasitic indices of ticks. A flock of 57 i... more The aim of this study was to identify the species and parasitic indices of ticks. A flock of 57 individuals (sheep, goats, cattle and dogs) in southern Algeria was studied from March 2019 to February 2020 during monthly visits. A total of 2544 ticks were collected by examining the entire body of the animal. Two tick species were identified: Hyalomma dromedarii (2430 ticks) and Rhipicephalus sanguineus sensu lato (114 ticks). The first species had annual activity and consisted only of adults, while the second species had spring and summer activity and contained both adults and nymphs. At the annual level, the prevalence of infestation, abundance, and intensity were 38.60%, 44.63 and 115.64 ticks per animal, respectively. At the monthly level there were species specific fluctuations, with a peak in activity in September.

Research paper thumbnail of Characterization of ovarian follicular and cystic fluids in cows

Veterinaria, 2018

The study was aimed at evaluating the hormonal, histological and metabolic changes associated wit... more The study was aimed at evaluating the hormonal, histological and metabolic changes associated with ovarian cyst (OC) formation in cows. For this purpose, , the fluid was aspirated from 195 ovarian cysts and 120 ovarian large follicles collected at a local abattoir in order to assess their hormonal concentrations and metabolic changes. Pieces of the cyst wall tissue were subjected to histologic evaluation. Data showed that the cysts’ fluid was characterized by the lower concentrations of glucose, cholesterol, total protein and higher urea levels than those of the large follicles (P 1, whereas the luteal cysts all had E/P ratio < 1. It can be speculated that the abnormal levels of some biochemical and hormonal parameters may lead to follicle dysfunction resulting in cyst formation. Keywords: ovary, cyst, biochemistry, hormone, cow

Research paper thumbnail of Agricultural Academy SEMEN PARAMETERS AND THEIR SEASONAL VARIATIONS OF LOCAL ARBIA BREED BUCKS IN WESTERN ALGERIA

Belhamiti, T.B., A. Ait Amrane, S.M. Hammoudi, S.M.A. Selles, A.R. Benia, E.A. Mammeri and R. Kai... more Belhamiti, T.B., A. Ait Amrane, S.M. Hammoudi, S.M.A. Selles, A.R. Benia, E.A. Mammeri and R. Kaidi, 2018. Semen parameters and their seasonal variations of local Arbia breed bucks in Western Algeria. Bulg. J. Agric. Sci.,

Research paper thumbnail of Ovarian cysts in cattle

Veterinarska stanica, 2021

Ovarian cysts (OC) are among the major factors affecting dairy cattle fertility due to their impa... more Ovarian cysts (OC) are among the major factors affecting dairy cattle fertility due to their impacts on reproductive performance. Extension of the calving-calving interval and the costs of treatmentassociated with this pathology are the main sources of economic losses for the dairy industry. This study involved conducting an epidemiological survey relating to ovarian cysts in cows based on the observations of veterinary practitioners in Algeria. This survey was performed using a questionnaire distributed to 103 practicing veterinarians in different regions of Algeria. According to the responses, OC dominated all ovarian diseases, followed by smooth ovaries and ovarian adhesions. Ovarian tumours were encountered very rarely. In general, veterinarians considered any follicular structure greater than 20 mm in diameter and persisting for at least 10 days to be cystic. The aetiology was multifactorial, and the following were cited: alimentation, high milk production, puerperal pathologie...

Research paper thumbnail of Investigation of the presence of slime production, VanA gene and antiseptic resistance genes in Staphylococci isolated from bovine mastitis in Algeria

Veterinarska stanica, 2020

Staphylococcus strains are frequently as- sociated with clinical and subclinical bovine intra-mam... more Staphylococcus strains are frequently as- sociated with clinical and subclinical bovine intra-mammary infection. The virulence factors of staphylococcus have not been widely studied in Algeria. The objective of this study was to determine the frequency of slime production, VanA gene and antiseptic resistance genes in staphylococci strains isolated from bovine mas- titis in Algeria. The study examined 35 Staphy- lococci strains obtained from the inflammatory secretion of mammary glands of cows with mastitis. Slime production was determined by detecting the icaA and icaD genes using the polymerase chain reaction (PCR) method and Congo red agar (CRA) method. The presence of qacAB and qac C antiseptic resistance genes and the VanA resistance gene in these isolates was investigated by PCR. The results of the current study revealed that of the 35 Staphylo- cocci isolates, 42.85% (15/35) and 17.14% (6/35) of the isolates harboured the slime production gene by analysing icaA and icaD genes,...

Research paper thumbnail of Alternative treatment of bovine mastitis

Veterinarska stanica, 2021

The treatment of mastitis is based mainly on the use of antibiotics. However, in recent times, an... more The treatment of mastitis is based mainly on the use of antibiotics. However, in recent times, an increase in resistance phenomena and the presence of residues in milk and their derivatives has been reported. Few studies have focused on the treatment of mastitis by homeopathy elsewhere or in Algeria. The objective of this current study aimed to clarify in particular the interest of homeopathy in the treatment of mastitis. The study was carried out on two dairy farms on a total of 14 cows located in Laghouat region (southern Algeria). Before the start of the experiment, a tolerance test was performed on two cull cows free from any apparent infection (except mastitis). Local and general reactions were noted at specific times. A total of 31 mammary quarters received 4 intramammary injections of a homeopathic preparation containing several natural products every 12h over 48h. A clinical examination and an analysis of the milk samples on D0, D7 and D14 were performed on all cows and the ...

Research paper thumbnail of Effects of GnRH or hCG on day 11 after artificial insemination in cows luteal activity

Journal of the Hellenic Veterinary Medical Society, Feb 5, 2019

In order to optimize luteal function, human chorionic gonadotrophin (hCG) or gonadotrophin releas... more In order to optimize luteal function, human chorionic gonadotrophin (hCG) or gonadotrophin releasing hormone (GnRH) were used on day 11 after artificial insemination (AI). 33 cows synchronized by the Ovsynch and divided into 3 groups according to the type of treatment: 1) hCG (1500 IU, n=11); 2) GnRH (100 µg, n=11) and 3) control (2 mL of saline, n=11). Blood samples were collected from all animals every 3 days from day 5 to day 23 to determine progesterone concentration. Ultrasonography was used to monitor the luteal surface structures at the time of blood sample collection. An accessory corpus luteum (CL) formed in 63.63% of cows treated with GnRH or hCG, resulting in an increase in the total luteal tissue area compared with the controls. Compared with the controls, the principal CL area was increased by hCG but not by GnRH. Additionally, compared with the control group, hCG-treated cows had increased progesterone concentrations (p<0.0001), while GnRH-treated cows had P4 similar to that of controls cows.

Research paper thumbnail of Development of in-house RIA kit for progesterone in cow’s skim milk

Journal of Immunoassay and Immunochemistry, 2019

A radioimmunoassay (RIA) method for progesterone has been developed. It has been validated for th... more A radioimmunoassay (RIA) method for progesterone has been developed. It has been validated for the determination of progesterone in cow skim milk. The main reagents used in the development work were prepared and characterized inhouse. The assay uses a radioiodinated tracer purified by gel filtration on Sephadex G-25, standards prepared in skim milk and coated tubes with specific antibodies as the solid phase (separation system). The radiochemical purity of the tracer was greater than 95%, the maximum binding using solid phase reached 43% and the nonspecific binding didn't exceed 5%. Series of progesterone standards using milk matrix with concentration ranging from 0 to 40ng/mL were prepared. Detection limit of the assay was 0.13ng/mL and the precision evaluation gives an intra and inter-assay coefficient variations between 4.94% and 12.66%. The recovery obtained with skim milk samples was 84% to 119% and the parallelism test indicated good linearity (R 2 > 0.99). The clinical tests give high correlation coefficient (r = 0.998) between progesterone concentrations of cow's skim milk assayed using developed progesterone RIA kit and commercial progesterone RIA kit.

Research paper thumbnail of Feeding practices of dairy cows in Algeria: Characterization, typology, and impact on milk production and fertility

Journal of Advanced Veterinary and Animal Research, 2019

Objective: To explore feeding strategies and identify eventual errors that could cause poor produ... more Objective: To explore feeding strategies and identify eventual errors that could cause poor production and reproduction performances in dairy farms in the north of Algeria. Materials and Methods: A survey was conducted among 211 farms of different sizes, from 2014 to 2018 to compose a database that is analyzed statistically. Results: The results relative to the nutritive value of the rations showed an average dry matter intake of 15.9 ± 4.74 kg/cow/day, providing 13.2 ± 4.34 UFL (Feed Unit for Lactation) of net energy and 1306 ± 456 gm of protein digested in small intestine (PDI)/cow/day. A high proportion of concentrate intake is observed, with an average of 64.7% ± 17.4% of energy intake and 70.2% ± 16.2% of nitrogen intake. Dairy cows performances were characterized by a low milk production regarding their genetic potential (14.2 ± 4.73 kg of milk/cow/day), a calving interval > to one year (397 ± 20.4 days), though the coverage rates of their nutrient requirements reached 120%. The typology of the dietary rations allowed grouping them into three categories: deficient rations cluster (DR), correct rations cluster, and excessive rations cluster (ER). DR and ER, which are not adjusted to animals' needs, were found in 57.8% of farms. Conclusion: It is obvious that the feeding management in the dairy farms in the North of Algeria is not based upon scientific achievements, contributing to relatively low performances of cows as well as to important financial losses. This finding imposes the necessity to adopt a correct and accurate rationing of animals.

Research paper thumbnail of Retrospective study of the reproductive performance of Barb and Thoroughbred stallions in Algeria

July-2019, 2019

Background and Aim: The Barb horse occupies a prominent place in the history, culture, and equest... more Background and Aim: The Barb horse occupies a prominent place in the history, culture, and equestrian traditions of the Maghreb (Algeria, Morocco, and Tunisia). Although many studies on the breed standard and morphometry have been published, there are no studies on its fertility and reproductive potential. Thus, this work aimed to study the fertility of Barb, Arabian Purebred, and Thoroughbred horses in Algeria. Materials and Methods: A total of 168 stallions and 1202 mares at the Chaouchaoua Stud farm in Tiaret, Algeria, were included in the study. The reproductive performance during 1592 cycles over 10 consecutive mating seasons (2003-2012) was evaluated. Multivariate analysis with logistic regression was used to analyze the reproductive parameters such as the number of cycles operated, number of pregnant mares, pregnancy rate per cycle, seasonal pregnancy rate, and embryonic mortality rate, and to determine the influence of breed, stallion's age, and year of the study on repr...

Research paper thumbnail of Comparative efficacy of two-dimensional mode and color Doppler sonography in predicting gender of the equine fetus

Veterinary World, 2019

Background and Aim: Ultrasonographic fetal sexing is of utmost economic importance for horse bree... more Background and Aim: Ultrasonographic fetal sexing is of utmost economic importance for horse breeders. Relatively, a few studies have been conducted to determine fetal sex in mare using transrectal Doppler ultrasound. This study aimed to compare two sexing techniques, two-dimensional (2D) mode and color Doppler ultrasonography. Materials and Methods: The study was conducted on 39 mares under field conditions. Examinations were performed using the ultrasonic model device (Medison SonoAce Pico, South Korea), equipped with real-time 3-7 MHz convex multifrequency transducer. Fetal sex diagnosis was carried out in two periods of pregnancy, early period (57-80 days of gestation) and late period (80-150 days of gestation). Results: No difference (p=0.4) was observed between the efficiency of the 2D mode and Doppler ultrasound in sex determination with the respective frequency of 74% and 85%. The best time to sex the early fetus was between 57 and 70 days of gestation and between 90 and 120...

Research paper thumbnail of Séroprévalence de la fièvre Q chez les bovins de la région de Bejaïa (Algérie)

Revue d’élevage et de médecine vétérinaire des pays tropicaux, 2017

La fièvre Q est une zoonose bactérienne causée par Coxiella burnetii, une bac­térie intracellulai... more La fièvre Q est une zoonose bactérienne causée par Coxiella burnetii, une bac­térie intracellulaire stricte, de répartition mondiale. Les ruminants domestiques sont le principal réservoir de la bactérie. L’objectif de cette étude a été de déter­miner la séroprévalence de la fièvre Q chez des bovins de la région de Bejaïa, au nord de l’Algérie. Un total de 180 sérums provenant de 50 élevages laitiers a été analysé par la technique Elisa (enzyme-linked immunosorbent assay). Dix-neuf sérums provenant de 11 élevages étaient positifs, soit une prévalence indi­viduelle de 10,6 % (intervalle de confiance [IC] à 95 %, 6,1 % à 15,0 %) et une séroprévalence troupeau de 22 % (IC 95 %, 10,5 % à 33,5 %). Une cor­rélation statistiquement significative a été mise en évidence entre la séropositi­vité et l’existence d’antécédents de rétention placentaire (p = 0,025), de métrites (p < 0,001) ou de retour régulier en chaleurs (p = 0,026) chez les vaches. La fièvre Q circule donc dans les élevages b...

Research paper thumbnail of Variations de l’état corporel d’ovins et systèmes d’élevage dans la région de Chlef, Algérie

Revue d’élevage et de médecine vétérinaire des pays tropicaux, 2017

L’état corporel de lots de brebis mises à la reproduction a été noté dans des exploitations de la... more L’état corporel de lots de brebis mises à la reproduction a été noté dans des exploitations de la région de Chlef en Algérie de février 2012 à avril 2013. L’état corporel et les réserves des brebis ont varié selon, d’une part, la catégorie des femelles concernées (agnelages en septembre-octobre ou en mars-avril) et, d’autre part, l’exploitation. La prise en compte des quantités de fourrage distribuées en complément du pâturage et des réserves corporelles a permis d’établir un diagnostic sur l’alimentation dans chaque situation. L’interaction entre l’état corporel des brebis mises à la lutte et les performances de reproduction a été mise en évidence. En effet, à mesure que la note d’état corporel (NEC) à la mise à la lutte augmentait, la fertilité et la prolificité s’amélioraient. Les brebis dont la NEC à la mise à la lutte a été supérieure ou égale à 3 ont obtenu des taux de fertilité de 100 % et de prolificité de 130 %, contre respectivement 76 % et 100 % pour celles dont la NEC à ...