Kaiyue Fu - Academia.edu (original) (raw)

Papers by Kaiyue Fu

Research paper thumbnail of Fire Hose Automatic Cleaning and Drying Machine Design and Analysis

2021 IEEE International Conference on Advances in Electrical Engineering and Computer Applications (AEECA), 2021

Solidworks 3D software is applied to establish the solid model of automatic fire hose cleaning an... more Solidworks 3D software is applied to establish the solid model of automatic fire hose cleaning and drying machine, introduce its working principle and process, use ANSYS-Workbench finite element software to carry out modal analysis on the frame of automatic fire hose cleaning and drying machine in general, calculate and analyze the first 6th order intrinsic frequency and corresponding modal vibration pattern, determine whether resonance occurs with ultrasonic cleaning frequency and brush cleaning frequency, optimize on this basis, and determine the feasibility of the scheme.

Research paper thumbnail of Rapid and selective recognition of Vibrio parahaemolyticus assisted by perfluorinated alkoxysilane modified molecularly imprinted polymer film

RSC Advances, 2020

Molecular imprinting technology offers a means of tailor-made materials with high affinity and se... more Molecular imprinting technology offers a means of tailor-made materials with high affinity and selectivity for certain analysts.

Research paper thumbnail of Development of a self-priming PDMS/paper hybrid microfluidic chip using mixed-dye-loaded loop-mediated isothermal amplification assay for multiplex foodborne pathogens detection

Analytica Chimica Acta, 2018

Development of a self-priming PDMS/paper hybrid microfluidic chip using mixeddye-loaded loop-medi... more Development of a self-priming PDMS/paper hybrid microfluidic chip using mixeddye-loaded loop-mediated isothermal amplification assay for multiplex foodborne pathogens detection,

Research paper thumbnail of Selective turn-on fluorescence detection of Vibrio parahaemolyticus in food based on charge-transfer between CdSe/ZnS quantum dots and gold nanoparticles

Food Control, 2017

Vibrio parahaemolyticus (V. parahaemolyticus) is one of the most common food-borne pathogens foun... more Vibrio parahaemolyticus (V. parahaemolyticus) is one of the most common food-borne pathogens found along the east coast of China, Japan, and several southeast Asian countries, where marine foods are frequently consumed. Thus it is of great interest to establish a fast, sensitive and robust analytical assay for V. parahaemolyticus detection. Herein, we report a V. parahaemolyticus-specific egg yolk antibody (IgY), extracted from the egg yolks of immunized hens with inactivated V. parahaemolyticus bacteria. By immobilizing IgY onto the surface of gold nanoparticles (AuNPs) through electrostatic self-assembly, a detection system was developed based on charge-transfer quenching fluorescence, which contains core/ shell CdSe/ZnS quantum dots (QDs), IgY-AuNPs and free AuNPs. In this strategy, the IgY-AuNPs were induced to aggregate quickly in the presence of target V. parahaemolyticus, resulting in a rapid fluorescence response of the QDs. More importantly, the convenient turn-on fluorescent detection system exhibited excellent selectivity over the other bacteria. Furthermore, this facile one-step method can detect V. parahaemolyticus at low concentrations of 10 cfu/mL without pre-enrichment and can be applicable for the detection of V. parahaemolyticus in real food samples. With the advantage of simplicity, rapidness, sensitivity, and specificity, this proposed approach shows promise as a successful application of V. parahaemolyticus in bioanalysis.

Research paper thumbnail of {"__content__"=>"Colorimetric Immunoassay for Rapid Detection of Vibrio parahemolyticus Based on Mn Mediates the Assembly of Gold Nanoparticles.", "sup"=>{"__content__"=>"2+"}}

Journal of agricultural and food chemistry, Jan 12, 2018

Vibrio parahemolyticus ( V. parahemolyticus) is an important food-borne pathogen that causes food... more Vibrio parahemolyticus ( V. parahemolyticus) is an important food-borne pathogen that causes food poisoning and acute gastroenteritis in humans. Herein, a novel colorimetric immunoassay was presented for rapid detection of V. parahemolyticus using gold nanoparticles (18.1 nm diameter) as chromogenic substrate, whose combination of a magnetic bead-based sandwich immunoassay and an optical sensing system via Mn ions mediated aggregation of gold nanoparticles. MnO nanoparticles coated with polyclonal IgG antibodies (7.8 nm diameter) are used to recognize the target and can be etched to generate manganese ions by ascorbic acid. A color change ranging from red to purple to blue can be easily discerned by bare eye, corresponding to V. parahemolyticus concentration in the range between 10 and 10 cfu·mL. The proposed method possesses high specificity with a limit of detection of 10 cfu·mL and was successfully applied to determination of V. parahemolyticus in oyster samples without pre-enric...

Research paper thumbnail of Rapid and Quantitative Detection of Vibrio parahemolyticus by the Mixed-Dye-Based Loop-Mediated Isothermal Amplification Assay on a Self-Priming Compartmentalization Microfluidic Chip

Journal of agricultural and food chemistry, Jan 27, 2017

Vibrio parahemolyticus (VP) mostly isolated from aquatic products is one of the major causes of b... more Vibrio parahemolyticus (VP) mostly isolated from aquatic products is one of the major causes of bacterial food-poisoning events worldwide, which could be reduced using a promising on-site detection method. Herein, a rapid and quantitative method for VP detection was developed by applying a mixed-dye-loaded loop-mediated isothermal amplification (LAMP) assay on a self-priming compartmentalization (SPC) microfluidic chip, termed on-chip mixed-dye-based LAMP (CMD-LAMP). In comparison to conventional approaches, CMD-LAMP was advantageous on the limit of detection, which reached down to 1 × 10CFU/mL in food-contaminated samples without the pre-enrichment of bacteria. Additionally, as a result of the use of a mixed dye and SPC chip, the quantitative result could be easily acquired, avoiding the requirement of sophisticated instruments and tedious operation. Also, CMD-LAMP was rapid and cost-effective. Conclusively, CMD-LAMP has great potential in realizing the on-site quantitative analysi...

Research paper thumbnail of Development of a low-cost paper-based ELISA method for rapid Escherichia coli O157:H7 detection

Analytical biochemistry, Feb 17, 2017

Escherichia coli O157: H7 (E. coli O157: H7) has become one of the most dangerous foodborne patho... more Escherichia coli O157: H7 (E. coli O157: H7) has become one of the most dangerous foodborne pathogenic bacteria around the world. Currently, because of the tedious, high-cost and stringent laboratory conditions required, the conventional E. coli O157: H7 detection methods, such as culture-based methods and polymerase chain reaction (PCR), have much limitation. Thus, we developed a novel paper-based enzyme-linked immunosorbent assay (p-ELISA) with shorter operation duration, lower cost, relatively higher sensitivity and wider application. This method required less than 3 h and 5 μL of sample to complete the detection. The limit of detection (LOD) for E. coli O157: H7 reached 1 × 10(4) CFU/mL with high specificity. To be more suitable for on-site testing, the readout could be rapidly obtained without any expensive instruments. In this study, we chose E. coli O157:H7 as the representative, and our method could provide a platform for determination of other pathogenic bacteria.

Research paper thumbnail of Fire Hose Automatic Cleaning and Drying Machine Design and Analysis

2021 IEEE International Conference on Advances in Electrical Engineering and Computer Applications (AEECA), 2021

Solidworks 3D software is applied to establish the solid model of automatic fire hose cleaning an... more Solidworks 3D software is applied to establish the solid model of automatic fire hose cleaning and drying machine, introduce its working principle and process, use ANSYS-Workbench finite element software to carry out modal analysis on the frame of automatic fire hose cleaning and drying machine in general, calculate and analyze the first 6th order intrinsic frequency and corresponding modal vibration pattern, determine whether resonance occurs with ultrasonic cleaning frequency and brush cleaning frequency, optimize on this basis, and determine the feasibility of the scheme.

Research paper thumbnail of Rapid and selective recognition of Vibrio parahaemolyticus assisted by perfluorinated alkoxysilane modified molecularly imprinted polymer film

RSC Advances, 2020

Molecular imprinting technology offers a means of tailor-made materials with high affinity and se... more Molecular imprinting technology offers a means of tailor-made materials with high affinity and selectivity for certain analysts.

Research paper thumbnail of Development of a self-priming PDMS/paper hybrid microfluidic chip using mixed-dye-loaded loop-mediated isothermal amplification assay for multiplex foodborne pathogens detection

Analytica Chimica Acta, 2018

Development of a self-priming PDMS/paper hybrid microfluidic chip using mixeddye-loaded loop-medi... more Development of a self-priming PDMS/paper hybrid microfluidic chip using mixeddye-loaded loop-mediated isothermal amplification assay for multiplex foodborne pathogens detection,

Research paper thumbnail of Selective turn-on fluorescence detection of Vibrio parahaemolyticus in food based on charge-transfer between CdSe/ZnS quantum dots and gold nanoparticles

Food Control, 2017

Vibrio parahaemolyticus (V. parahaemolyticus) is one of the most common food-borne pathogens foun... more Vibrio parahaemolyticus (V. parahaemolyticus) is one of the most common food-borne pathogens found along the east coast of China, Japan, and several southeast Asian countries, where marine foods are frequently consumed. Thus it is of great interest to establish a fast, sensitive and robust analytical assay for V. parahaemolyticus detection. Herein, we report a V. parahaemolyticus-specific egg yolk antibody (IgY), extracted from the egg yolks of immunized hens with inactivated V. parahaemolyticus bacteria. By immobilizing IgY onto the surface of gold nanoparticles (AuNPs) through electrostatic self-assembly, a detection system was developed based on charge-transfer quenching fluorescence, which contains core/ shell CdSe/ZnS quantum dots (QDs), IgY-AuNPs and free AuNPs. In this strategy, the IgY-AuNPs were induced to aggregate quickly in the presence of target V. parahaemolyticus, resulting in a rapid fluorescence response of the QDs. More importantly, the convenient turn-on fluorescent detection system exhibited excellent selectivity over the other bacteria. Furthermore, this facile one-step method can detect V. parahaemolyticus at low concentrations of 10 cfu/mL without pre-enrichment and can be applicable for the detection of V. parahaemolyticus in real food samples. With the advantage of simplicity, rapidness, sensitivity, and specificity, this proposed approach shows promise as a successful application of V. parahaemolyticus in bioanalysis.

Research paper thumbnail of {"__content__"=>"Colorimetric Immunoassay for Rapid Detection of Vibrio parahemolyticus Based on Mn Mediates the Assembly of Gold Nanoparticles.", "sup"=>{"__content__"=>"2+"}}

Journal of agricultural and food chemistry, Jan 12, 2018

Vibrio parahemolyticus ( V. parahemolyticus) is an important food-borne pathogen that causes food... more Vibrio parahemolyticus ( V. parahemolyticus) is an important food-borne pathogen that causes food poisoning and acute gastroenteritis in humans. Herein, a novel colorimetric immunoassay was presented for rapid detection of V. parahemolyticus using gold nanoparticles (18.1 nm diameter) as chromogenic substrate, whose combination of a magnetic bead-based sandwich immunoassay and an optical sensing system via Mn ions mediated aggregation of gold nanoparticles. MnO nanoparticles coated with polyclonal IgG antibodies (7.8 nm diameter) are used to recognize the target and can be etched to generate manganese ions by ascorbic acid. A color change ranging from red to purple to blue can be easily discerned by bare eye, corresponding to V. parahemolyticus concentration in the range between 10 and 10 cfu·mL. The proposed method possesses high specificity with a limit of detection of 10 cfu·mL and was successfully applied to determination of V. parahemolyticus in oyster samples without pre-enric...

Research paper thumbnail of Rapid and Quantitative Detection of Vibrio parahemolyticus by the Mixed-Dye-Based Loop-Mediated Isothermal Amplification Assay on a Self-Priming Compartmentalization Microfluidic Chip

Journal of agricultural and food chemistry, Jan 27, 2017

Vibrio parahemolyticus (VP) mostly isolated from aquatic products is one of the major causes of b... more Vibrio parahemolyticus (VP) mostly isolated from aquatic products is one of the major causes of bacterial food-poisoning events worldwide, which could be reduced using a promising on-site detection method. Herein, a rapid and quantitative method for VP detection was developed by applying a mixed-dye-loaded loop-mediated isothermal amplification (LAMP) assay on a self-priming compartmentalization (SPC) microfluidic chip, termed on-chip mixed-dye-based LAMP (CMD-LAMP). In comparison to conventional approaches, CMD-LAMP was advantageous on the limit of detection, which reached down to 1 × 10CFU/mL in food-contaminated samples without the pre-enrichment of bacteria. Additionally, as a result of the use of a mixed dye and SPC chip, the quantitative result could be easily acquired, avoiding the requirement of sophisticated instruments and tedious operation. Also, CMD-LAMP was rapid and cost-effective. Conclusively, CMD-LAMP has great potential in realizing the on-site quantitative analysi...

Research paper thumbnail of Development of a low-cost paper-based ELISA method for rapid Escherichia coli O157:H7 detection

Analytical biochemistry, Feb 17, 2017

Escherichia coli O157: H7 (E. coli O157: H7) has become one of the most dangerous foodborne patho... more Escherichia coli O157: H7 (E. coli O157: H7) has become one of the most dangerous foodborne pathogenic bacteria around the world. Currently, because of the tedious, high-cost and stringent laboratory conditions required, the conventional E. coli O157: H7 detection methods, such as culture-based methods and polymerase chain reaction (PCR), have much limitation. Thus, we developed a novel paper-based enzyme-linked immunosorbent assay (p-ELISA) with shorter operation duration, lower cost, relatively higher sensitivity and wider application. This method required less than 3 h and 5 μL of sample to complete the detection. The limit of detection (LOD) for E. coli O157: H7 reached 1 × 10(4) CFU/mL with high specificity. To be more suitable for on-site testing, the readout could be rapidly obtained without any expensive instruments. In this study, we chose E. coli O157:H7 as the representative, and our method could provide a platform for determination of other pathogenic bacteria.