Kamirou Chabi-Sika - Academia.edu (original) (raw)

Papers by Kamirou Chabi-Sika

Biotechnology journal international, Dec 31, 2022

Aims: This work aims to evaluate the antimicrobial activity of Sarcocephalus latifolius extracts.... more Aims: This work aims to evaluate the antimicrobial activity of Sarcocephalus latifolius extracts. Methodology: Thus, phytochemical screening was qualitatively accessed using colorations or precipitations methods. Ethanolic and aqueous extracts were used to evaluate the antimicrobial activity. The antimicrobial activity, using the diffusion method, was evaluated on eight strains including two reference strains (Streptococcus pneumoniae ATCC 49619 and Pseudomonas aeruginosa ATCC 27853) and six clinically isolated S. pneumoniae and P. aeruginosa strains. The minimum inhibitory concentrations (MIC) and minimum bactericidal concentrations (MBC) were determined by the microdilution method. Results: The phytochemical screening showed the presence of flavonoids, anthocyanins, mucilages, saponosides, C-heterosides and O-heterosides. Antimicrobial activity showed that the ethanolic extract with the lowest MIC (1.25 mg/ml) inhibited reference strains (S. pneumoniae ATCC 49619 and P. aeruginosa ATCC 27853) and clinical isolated S. pneumoniae and P. aeruginosa strains. The largest inhibition diameter (19± 1.33) was obtained with the ethanolic extract against clinical isolated Pseudomonas aeruginosa and (15.5± 1) against the reference one. The aqueous extract inhibited only reference strains. Conclusions: The data of this study indicate that the extracts of S. latifolius present antimicrobial properties. This may justify its traditional use in the treatment of microbial infections.

International Journal of Biochemistry Research & Review

Aims: Ageratum conyzoides L. is a small annual herbaceous highly odorous plant use in traditional... more Aims: Ageratum conyzoides L. is a small annual herbaceous highly odorous plant use in traditional medicine. The aim of this study is to evaluate in vitro antioxidant potential, toxicity and antimicrobial activity of aerial part extracts of A. conyzoides on strains potentially involved in vaginal infections. Methodology: An ethnobotanical survey has been carried out on A. conyzoides among ethnobotanists and traditional therapists in fifteen markets in the communes of Abomey- Calavi, Cotonou, Zogbodomey, Bohicon and Abomey in Southern Benin. The phytochemical screening was a qualitative analysis based on staining and precipitation reactions. Antimicrobial activity of A. conyzoides aqueous and ethanolic extracts was evaluated on reference and clinical strains of Staphylococcus aureus, Candida albicans and Escherichia coli using micro dilutions method in wells from. The toxicity of A. conyzoides extracts was determine using Artemia salina larvae, whereas the antiradical activity was eva...

Journal of Food Quality, Oct 11, 2022

P.butyracea butter, produced by different traditional methods, is often stored for further use in... more P.butyracea butter, produced by different traditional methods, is often stored for further use in different types of packaging which may affect its quality. e present work aims to evaluate the effect of the production method and the type of packaging used on the physicochemical and microbiological quality of butter during storage. e extraction of Pentadesma butter was first carried out through production monitoring in three repetitions by three different butter producers according to the two most used traditional production methods. en, butter from production was stored for three months in four types of packaging (aluminium bowls, calabashes, baskets, and black polyethylene bags) in the production environment. e microbiological and physicochemical quality of the stored butter was assessed at 0, 30, 60, and 90 days using normative reference methods. e production method and the type of packaging used had a significant effect on the variation of free fatty acid content (1.54 ± 0.07%-2.6 ± 0.2%), peroxide value (0.96 ± 0.09°meq•O 2 /Kg-3.9 ± 0.7°meq•O 2 /Kg), and colour of the butter during storage. In contrast, only the type of packaging material influenced the microbiological characteristics of the butter during storage. After three months of storage, the yeast and mould load was out of the standard range in all packages, i.e., 2.53 ± 0.4 log°CFU/g, 2.9 ± 0.2 log°CFU/g, 4.67 ± 0.2 log°CFU/g, and 1.4 ± 0.2 log°CFU/g for aluminium bowls, calabashes, baskets, and black polyethylene bags, respectively. e aerobic mesophilic germ load was within the standard in black polyethylene bags (3.22 ± 0.08 log°CFU/g), in contrast to the other packages (4.23 ± 0.08 log°CFU/g-6.45 ± 0.13 log°CFU/g). is shows that black polyethylene bags are the best packaging to guarantee the quality of butter. It is important to continue this investigation by storing butter for a longer period of time with more appropriate packaging.

International Journal of Zoology

The gut microbiota of mosquitoes is composed of a range of microorganisms. Among its microorganis... more The gut microbiota of mosquitoes is composed of a range of microorganisms. Among its microorganisms, some affect the vectorial capacity of mosquitoes. The aim of this study was to characterize some bacteria of the intestinal microbiota in Anopheles gambiae (An. gambiae) females, a major vector of malaria transmission in Benin. The symbiote bacteria of the microbiota of female laboratory An. gambiae and female wild An. gambiae were identified by the culture method. The count was done on media plate count agar (PCA), and subsequently, the bacterial load was calculated. Comparison of batches bacterial load was carried out with the variance analysis test (ANOVA). Finally, polymerase chain reaction (PCR) was performed to investigate the presence of a few bacterial genera influencing the vector capacity of An. gambiae. The study found that the microbiota of female An. gambiae is home to the bacteria belonging to the Staphylococcus, Enterobacteriaceae, and other unidentified bacterial gene...

African Journal of Biotechnology, 2021

The quality and composition of meat and its derivatives are influenced by many factors namely phy... more The quality and composition of meat and its derivatives are influenced by many factors namely physicochemical, organoleptic factors and microbial contamination. The aim of this study was to evaluate kpakouma consumption risk through its chemical and microbiological contaminants. The methodological approach was composed of (i) observation of selling environment, (ii) pesticides and antibiotics residues quantification by HPLC, and (iii) microbiological analysis using selective media and biochemical tests. The data shows that aminoglycosides, penicillin and nitrofuran were not determined both in the black and the white kpakouma. Macrolides (0.094±0.004) and beta lactams (0.016±0.0036) are noted only with white kpakouma. Lindane (0.215±0.003) and HCH (1.0003±0.003) were only detected among some samples whereas chlorpyrifos, malathion and parathion were not detected in all the tested samples. Concerning the microbial contaminants, according to the European Regulation, all kpakouma samples were highly contaminated with Staphylococcus species, Escherichia coli and Salmonella. The isolated Staphylococcus spp. were mostly (90%) resistant to vancomycin, no Staphylococcus spp. resistance was recorded for ciprofloxacin. E. coli and Salmonella were all resistant to oxytetracycline, no resistant isolate of E. coli was recorded for ciprofloxacin but Salmonella strains were at 25% resistant to ciprofloxacin. These results show the non-compliance with the hygiene rules during the sale of kpakouma and reaffirm the potentially critical role that can be played by commensals in public health.

Journal of Pharmacognosy and Phytochemistry, 2020

Many studies have been done on Garcinia kola seeds. They have not indicated the importance of the... more Many studies have been done on Garcinia kola seeds. They have not indicated the importance of the coats on biological activities of these seeds. Thus, we studied the impact of the use of Garcinia kola seeds with its teguments. We studied the comparative phytochemical and antibacterial activities of Garcinia kola seeds with and without tegument. First, the qualitative analysis of ethanolic extract of peeled and unpeeled seeds revealed the presence of large families of molecules such as alkaloids, saponins, flavonoids and phenolic compounds consisting of catechic and gallic tannins in both extracts. Additional compounds namely anthocyanins and O-heterosides had been identified in unpeeled seeds extract. The difference noted during the quantitative analysis of the two extracts is not significant (p ˃ 0.05). Then, antimicrobial tests had shown higher inhibition diameters with peeled seeds in 70% of the cases. The largest inhibition diameter was 35 mm obtained against Proteus vulgaris. T...

Asian Food Science Journal, 2021

Introduction: Staphylococcus aureus is one of the main pathogens found in street food, including ... more Introduction: Staphylococcus aureus is one of the main pathogens found in street food, including hot beverages. However, information about S. aureus isolated from street hot beverages from coffee carts is very limited in Abidjan, Côte d’Ivoire. Aims: We aimed to characterize phenotypically S. aureus isolated from street hot beverages sold in Abidjan. Methodology: A total of 400 samples of hot beverage were collected and analyzed. The identification was made through conventional microbial and biochemical analysis. Macroscopic identification on the Baird Parker agar supplement with egg yolk tellurite. Microscopic observation through Gram staining as well as biochemical tests such as catalase, DNase and coagulase were performed. To confirm staphylococcal strains, the MALDI-TOF mass spectrometry was used. After identification, the susceptibility of the staphylococcal isolates was evaluated using disc diffusion method. Results: Result showed that most of Staphylococcus aureus (18.4%) wer...

International Journal of Microbiology, 2020

Staphylococcus spp. is most often implicated in nosocomial infections. The objective of this stud... more Staphylococcus spp. is most often implicated in nosocomial infections. The objective of this study is to evaluate the susceptibility to antibiotics and the biofilm formation capacity of staphylococci species isolated from surfaces and medicotechnical materials at the university hospital center of Abomey-Calavi/Sô-Ava in Benin. Samples were collected according to ISO/DIS14698-1 standard from the surfaces and medicotechnical materials by the dry swab method. The isolation of Staphylococcus strains was performed on Chapman agar, and their identification was performed using microscopic and biochemical methods. The susceptibility of Staphylococcus isolates to antibiotics was evaluated by the disc diffusion method according to EUCAST and CLSI recommendations. The biofilm formation was qualitatively assessed using microplates. Of the 128 surfaces and medicotechnical material samples analyzed, 77% were contaminated with Staphylococcus spp. Thirteen species of Staphylococcus were isolated in...

European Scientific Journal, 2017

The aim of this work was the melissopalynologycal analysis of the honey samples collected both du... more The aim of this work was the melissopalynologycal analysis of the honey samples collected both during the dry and rainy seasons of the three phyto-geographical zones of Benin. The analysis was performed by conventional methods on 60 honeys samples. The total of 138 taxa have been identified at family level (31), genus level (20) and to the level species (87). The number of pollens counted varies according to the phyto-geographical zones. Thus, 17091 pollens have been counted in the samples of the Sudanian zone; 13884 in those of the Sudano-Guinean zone and 7960 pollens in those of the Guinean zone. The pollen content of the honey samples reflects the plant species of the three phyto-geographical zones. The most dominant plant taxa were Combretaceaes (26.01%) and Parkia biglobosa (10.67%) in Sudanian zone, Combretaceae (29.52%) in SudanoGuinean zone. In the Guinean zone, the dominant taxa were Zea mays (18.35%) and Terminalia (15.34%). The specific richness varies from 6 to 43 in the...

Journal of Plant Breeding and Crop Science, 2017

A better approach to assess plant genetic diversity is the agro-morphological characterization. T... more A better approach to assess plant genetic diversity is the agro-morphological characterization. The main objective of this study was to investigate the morphological variability of 87 maize (Zea mays L.) accessions collected in different agro-ecological zones of southern Benin. Thus, 16 agro-morphological characters (seven quantitative and nine qualitative) were selected from the maize descriptors. The experimental design used is an incomplete randomized block with three replications. The mixed model analysis of two factors variance revealed a very highly significant difference for all accessions for each quantitative agro-morphological characteristic evaluated. The numerical classification of all corn accessions revealed five groups of accessions. The results of the stepwise discriminant analysis revealed five agro-morphological characteristics (germination days, female flowering, plant height, ear height and sensitivity to streak) most discriminating. The results of numerical classification supervised based on the most discriminating variables enable identification of the best accessions. Comparison of the two types of accessions (local and improved) revealed a significant difference among of them for agro-morphological characteristics with some exception. The groups obtained can constitute a database for breeders in a maize breeding program.

American Journal of Molecular Biology, 2016

Maize (Zea mays L.) is the major cereal cultivated in Benin and it is important to know its genet... more Maize (Zea mays L.) is the major cereal cultivated in Benin and it is important to know its genetic diversity to improve the yield. The genetic markers of important traits are evaluated in order to improve the maize inbred lines. The aim of this study was to evaluate the genetic diversity of Benin's maize accessions by SSR marker. Thus, one hundred eighty seven maize accessions from three areas (South, Center and North) were analyzed using three SSR markers. A total of 227 polymorphic bands were produced and showed high genetic diversity (Shannon index = 0.51). The polymorphic information content (PIC) values for the SSR loci ranged from 0.58 to 0.81, with an average of 0.71. Genetic distance-based UPGMA dendrogram showed a genetic differentiation between accessions and they were grouped into four clusters in each area. This work provides necessary information that can be used not only to improve the maize production and conservation but also to better manage genetic species resources in Benin.

Plant Gene, 2015

Extraction of high quality genomic DNA from higher plants is hindered by the presence of secondar... more Extraction of high quality genomic DNA from higher plants is hindered by the presence of secondary metabolites, which reduce the yield and quality of the DNA. We describe an alternative protocol for genomic DNA extraction from fresh and dry plant leaves that is amenable to PCR-based genetic analysis. Existing methods were either very lengthy, expensive or not suitable for extraction of genomic DNA from dry leaves. Our method used SDS and high salt concentrations to extract DNA and does not require use of hazardous materials or special laboratory equipment. Genomic DNA extracted using our method was used for PCR-based genetic characterization of different varieties of cashew trees, Anacardium occidentale, via SSR markers as well as Zea mays varieties. This protocol improves existing methods in that it has the advantage of being adaptable to studies with a large number of samples and limited resources. The method is rapid, cost efficient and uses non-hazardous reagents. Genomic DNA extracted using this method has sufficient quality for downstream PCR-based genetic analysis.

Asian Journal of Biology

Aims: The aim of this study is to evaluate the antimicrobial activity of the R. brasiliensis aeri... more Aims: The aim of this study is to evaluate the antimicrobial activity of the R. brasiliensis aerial part extracts collected in southern-Benin. Methodology: The phytochemical screening was performed by a differential precipitation staining method. Aqueous and ethanolic extracts were made using conventional method with water and ethanol as solvent. The obtained extracts were used to evaluate their antimicrobial activity on Staphylococcus aureus strains isolated from skin infections and ten reference strains by the solid-medium diffusion method. The minimum inhibitory concentrations (MIC) and minimum bactericidal concentrations (MBC) were determined by the liquid macro-dilution method. The cytotoxic effect of the extracts was evaluated on Artemia salina larvae obtained by hatching. Results: The phytochemical screening showed a strong presence of tannins, flavonoids, terpenes, steroids and a medium presence of alkaloids, anthocyanins and mucilage’s. The extraction yields vary according ...

Asian Journal of Biology, 2021