Kansiree Jindapunnapat - Academia.edu (original) (raw)

Uploads

Papers by Kansiree Jindapunnapat

Nematology

Summary The legume cavalcade, Centrosema pascuorum, is used extensively as a cover crop and as a ... more Summary The legume cavalcade, Centrosema pascuorum, is used extensively as a cover crop and as a component of conservation agriculture systems. It is also an attractive rotation or cover crop for the management of root-knot nematodes (RKN; Meloidogyne spp.) as it is a non-host. RKN are persistent pests that are well known to be difficult to control. However, the mechanisms governing the non-host status of cavalcade is unknown. The current study established that cavalcade leaves are toxic to RKN as either aqueous extracts or soil amendments. Bioassays conducted using Meloidogyne javanica showed that a 90% concentration of aqueous extract derived from 1-month-old cavalcade leaves (89 mg crude extract ml−1) suppressed nematode hatch (82.9%) and killed infective second-stage juveniles of M. javanica (85.3%). Soil amendments with 1% (w/w) of 1-month-old cavalcade leaves (0.99 mg crude extract g−1 soil) also provided effective control of M. javanica in the glasshouse on okra. One-month-ol...

Journal of Phytopathology

Samanea saman (Jacq.) Merr., commonly known as rain tree, is a crucial shade tree with traditiona... more Samanea saman (Jacq.) Merr., commonly known as rain tree, is a crucial shade tree with traditional medicinal properties. In our study, 50 soil samples were collected around the rhizosphere of rain tree in Bangkok, Pathum Thani, Uttaradit, Khon Kaen and Ubon Ratchathani Provinces in Thailand. Meloidogyne populations were only found in soil samples from Mueang district, Khon Kaen Province and a 30‐year‐old rain tree exhibited root gall symptoms caused by root‐knot nematodes (Meloidogyne) on its adventitious roots. The species of the nematodes were identified based on its morphological and molecular characteristics. The results demonstrated that the perineal patterns and morphometrics of the nematodes were highly similar to the original description of M. enterolobii, except for a squarish dorsal arch and two faint lateral lines. During the molecular analysis, nematode DNA was extracted and amplified using three primer sets, C2F3/1108, D2A/D3B and MK7F/MK7R SCAR primer, to generate poly...

Zenodo (CERN European Organization for Nuclear Research), Dec 4, 2022

Journal of Developments in Sustainable Agriculture, 2013

and Ratchaburi provinces, which are in the central region of the country, are the largest areas f... more and Ratchaburi provinces, which are in the central region of the country, are the largest areas for guava cultivation. Kimju and Pansithong are the most extensively grown cultivars, and they have been seriously damaged by two species of root-knot nematodes, Meloidogyne incognita and M. enterolobii (or M. mayaguensis). The latter species was only recently reported in Thailand but is common in China, Brazil, and the United States. Substantial numbers of guava trees are in decline and show reduced fruit production. In this survey study of six guava orchards in central Thailand, we found that common aboveground symptoms of nematode infection included yellowing, stunting, folded leaves, blighted and wilted leaves (especially when water was lacking), and slow ripening of fruits. Underground symptoms included root galls, partially rotted roots, and, in some cases, prolific production of adventitious roots to compensate for damaged and nonfunctional roots. When we examined the morphometric characteristics of second-stage juveniles of M. incognita and M. enterolobii obtained from soil around guava plants, we observed no significant correlation between the characteristics of the two species or between the characteristics of populations within each species (P＞0.05). For the biological control of root-knot nematodes in guava, we evaluated a commercially available fungal agent, Trichoderma harzianum, which effectively controls several other soil-borne pathogens and has been shown to induce disease resistance and stimulate adventitious root growth in plants. We found that inoculation of the root zones of guava plants with T. harzianum reduced the number of nematodes in both soil and roots as compared to the number in untreated plants (P＜0.05). Moreover, inoculation of guava plants with T. harzianum arrested the development of the juvenile nematodes (P＜0. 05). Trichoderma harzianum is less expensive than chemical control agents and poses no risk to the environment.

Journal of the Saudi Society of Agricultural Sciences, 2021

The rice root nematodes, Hirschmanniella spp., are considered the predominant plant-parasitic nem... more The rice root nematodes, Hirschmanniella spp., are considered the predominant plant-parasitic nematode in the clay soils of Battambang’s lowland rice fields in Cambodia. In this study, we compared the nematode population dynamics, rice yield parameters, and soil organic matter content in lowland rice fields under conservation agriculture (CA) system with conventional tillage systems with green manure management (GMCT) or with plough-based tillage (CT) systems. Results demonstrated that GMCT for one year (GMCT1) and the long-term CA for seven years (CA7) reduced nematode densities in both soil and in rice roots, almost throughout the study period, compared to the CT. In the GMCT with tillage for two years (GMCT2), however, the Hirschmanniella spp. densities were high at the beginning, but reduced at later stages of the cycle. For rice yield components and soil fertility, CA7 proved to be effective in increasing plant height, the number of tillers/plant and soil organic matter. Based ...

Nematropica, 2019

Vetiver grass (Vetiveria zizanioides) is widely planted in tropical areas, and has many uses, inc... more Vetiver grass (Vetiveria zizanioides) is widely planted in tropical areas, and has many uses, including application of shoots as a mulch or soil amendment. Vetiver produces compounds that are active against nematodes and various cultivars are resistant to Meloidogyne spp. (root-knot nematodes). The commercially available vetiver cv. Sierra was tested for host status to Meloidogyne incognita and found to be resistant. To determine effects of vetiver soil amendments on vegetable crops, we conducted greenhouse trials with seedlings of cucumber, pepper, and tomato transplanted into soil that had been mixed with chopped, fresh vetiver shoots at 0%, 3%, and 5% g fresh vetiver/g dry soil (weight/weight). Results varied with time of amendment, amount of vetiver green manure, and plant species. Cucumber seedling response varied from no significant effect to some phytotoxicity. Tomato seedlings had lower shoot heights and root fresh weights in higher vetiver amendment rates. Pepper roots tend...

Trends in Sciences

The rice root nematodes, Hirschmanniella oryzae and H. mucronata, are serious and widespread thre... more The rice root nematodes, Hirschmanniella oryzae and H. mucronata, are serious and widespread threats to global rice production, especially in tropical and subtropical zones. Reliable and efficient identification of these nematodes is vital to nematode control and management. In this current study, 36 soil or rice root samples were collected from rice fields located in Pathum Thani province and used for nematode extraction. The results demonstrated that Hirschmanniella sp. was found in all paddy fields, especially in Muang District, where RD47 rice cultivars were grown at soil pH of 5.9. Molecular identification based on the analysis of 28S rRNA and 18S-ITS1-1.58S genes revealed 98 – 99 % similarity to H. mucronata, and its phylogenetic trees were grouped with H. mucronata from Cambodia and Philippines. Likewise, the morphometric characterization revealed remarkable features of H. mucronata with long body (1,639 - 2,329 µm), long stylet (24 - 27 µm) and an obvious mucron at the end o...

Journal of Nematology

Vetiver, a nonhost grass for certain nematodes, was studied for the production of compounds activ... more Vetiver, a nonhost grass for certain nematodes, was studied for the production of compounds active against the southern root-knot nematode, Meloidogyne incognita. In laboratory assays studying the effects on second-stage juvenile (J2) activity and viability, crude vetiver root and shoot extracts were nematotoxic, resulting in 40% to 70% J2 mortality, and were also repellent to J2. Vetiver oil did not exhibit activity against J2 in these assays. Gas chromatography-mass spectrometry analyses of three crude vetiver root ethanol extracts and a commercial vetiver oil determined that two of the major components in each sample were the sesquiterpene acid 3,3,8,8-tetramethyltricyclo[5.1.0.0(2,4)]oct-5-ene-5-propanoic acid and the sesquiterpene alcohol 6-isopropenyl-4,8a-dimeth yl-1,2,3,5,6,7,8,8a-octahydronaphthalen-2-ol. The acid was present in higher amounts in the extracts than in the oil. These studies demonstrating nematotoxicity and repellency of vetiver-derived compounds to M. incognita suggest that plant chemistry plays a role in the nonhost status of vetiver to root-knot nematodes, and that the chemical constituents of vetiver may be useful for suppressing nematode populations in the soil.