Khosro Piri - Academia.edu (original) (raw)

Papers by Khosro Piri

Research paper thumbnail of Effects of natural extract of Rosmarinus officinalis L., Thymus vulgaris L. and benzyl adenine hormone on some quality traits and shelf-life of Polianthes tuberosa L. cut flowers

Iranian Journal of Medicinal and Aromatic Plants, 2018

Research paper thumbnail of Developing an efficient protocol for hairy root induction in Lythrum salicaria L

Research paper thumbnail of Adventitions shoot formation from leaf explants of oleaster (Elaeagnus angustifolia L.)

Propagation of Ornamental Plants, 2009

Department of �iote��nolog�, �a��lt� of �gri��lt�re, ��-�li �ina �ni�ersit�, �ama�an, �ran of �io... more Department of �iote��nolog�, �a��lt� of �gri��lt�re, ��-�li �ina �ni�ersit�, �ama�an, �ran of �iote��nolog�, �a��lt� of �gri��lt�re, ��-�li �ina �ni�ersit�, �ama�an, �ran of �iote��nolog�, �a��lt� of �gri��lt�re, ��-�li �ina �ni�ersit�, �ama�an, �ran �iote��nolog�, �a��lt� of �gri��lt�re, ��-�li �ina �ni�ersit�, �ama�an, �ran �iote��nolog�, �a��lt� of �gri��lt�re, ��-�li �ina �ni�ersit�, �ama�an, �ran , �a��lt� of �gri��lt�re, ��-�li �ina �ni�ersit�, �ama�an, �ran �a��lt� of �gri��lt�re, ��-�li �ina �ni�ersit�, �ama�an, �ran of �gri��lt�re, ��-�li �ina �ni�ersit�, �ama�an, �ran of �gri��lt�re, ��

Research paper thumbnail of Sixteen Year Analyses of Burned Areas in Iran Based on Provincial Borders and Land Use with the Aim of Prioritizing and Managing Fires: A Case Study

Research Square (Research Square), Feb 1, 2023

In the present research, the burned area of each province was investigated according to land use ... more In the present research, the burned area of each province was investigated according to land use in order to manage and prioritize provinces with extent and high-risk burns. The analyses of burned areas in Iran has been ful lled in Iran for the rst time. Following the expansion of agricultural lands around forests, the number of intentional wild res aiming at reducing forest area has increased. Only 5% of wild res in the Mediterranean region have a natural origin. In Iran, the nancial damage caused by wild res (apart from spiritual and intangible damages such as woods, bushes, and herbal plants) between 1968 and 2007 has been estimated at about 1.93 million dollars. Therefore, prioritizing and managing re in natural ecosystems seems to be essential. In the present study, the burned areas of Iran from 2002-03-21 to 2018-03-21 based on land use were obtained from the data available in the Global Wild re Information System and were categorized by provincial borders. In order to prepare Iran's land use map, related data (MCD12Q1) were obtained from https://www.earthdata.nasa.gov in HDF5 format. The results showed that Khuzestan, Fars, Kermanshah, and Golestan Provinces, respectively had the largest burned areas in the country. Considering that most burned areas of the country have been recorded in agricultural lands, human factors are very in uential in increasing the rate of burned areas, and maintaining distance between agricultural lands and native vegetation (fuel) is mandatory.

Research paper thumbnail of Antifungal Effects of Two Medicinal Plant Native to Iran

International journal of Advanced Biological and Biomedical Research, Apr 1, 2014

Objective: In order to use natural compounds in controlling plant pests and diseases, many resear... more Objective: In order to use natural compounds in controlling plant pests and diseases, many researchers in recent years have studied the antifungal effects of essential oils and plant extracts. The purpose of this study was to investigate the chemical composition and antifungal effects of Echium khuzestanium and marubbium anisodan extracts. Methods: After extraction and preparation of different concentrarion from extracts, antifungal effect on 4 plant fungi was stadied by disc diffusion method. chemical composition of plants was checked by GC/MS. Results: According to the results, 86 kinds of chemical compounds found in M.anisodan extract. Furfural, steroids, vitamin B and flavonoids are the main compounds of M.anisodan. 46 kinds of chemical compounds found in methanol extract of E.khuzestanium. there are mucilage, fatty acids, flavonoid and diterpenes in flower of E.khuzestanium.

Research paper thumbnail of Anticancer, antioxidant and antibacterial activities of extracts from Scrophularia striata and Elaeagnus angustifolia, growing in Ilam and Kurdistan provinces in Iran

Plant science today, Nov 9, 2022

Emerging evidence of the impact of plant compounds on growth inhibition of micropathogens and can... more Emerging evidence of the impact of plant compounds on growth inhibition of micropathogens and cancer cells has opened new areas to evaluate plants' treatment properties. Here, we aimed to investigate in vitro antioxidant, antibacterial and anticancer effects of the secondary metabolites isolated from different extracts produced by Elaeagnus angustifolia and Scrophularia striata. Antibacterial activity was evaluated against human and plant pathogenic bacteria by 3 methods of tubular dilution, well and disc diffusion. The anticancer effect of E. angustifolia extract was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Biochemical experiments showed the presence of compounds such as phenol, flavonoids, resins, quinones, steroids, terpenoids and alkaloids in extracts, with the highest antioxidant activity of the methanolic extracts. For both plants' strains, the disc method was more effective than the well diffusion method. The highest yields were obtained from Methanolic, ethanolic and hydroalcoholic extracts for E. angustifolia and aqueous extract for Scrophularia striata. The most sensitive bacteria for E. angustifolia were Bacillus subtilis and Xanthomonas campestris against pit extracts and Clavibacter michiganensis against pulp extracts. The most sensitive bacteria for S. striata were Bacillus subtilis and Staphylococcus aureus. Methanolic and aqueous solvents showed the maximum bacterial inhibitory and bactericidal activities in the Minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) tests respectively. Additionally, E. angustifolia showed anticancer effects toward MCF7 breast cancer cells. These findings provided a better understanding of the widespread application of these plants as potential antioxidants, antibacterial and anticancer sources, and safe natural medicines in health maintenance and disease treatment.

Research paper thumbnail of Comparative evaluation of the antioxidant potential of some Iranian medicinal plants

Food Chemistry, 2007

Medicinal plants are a source for a wide variety of natural antioxidants. In the study reported h... more Medicinal plants are a source for a wide variety of natural antioxidants. In the study reported here, we have conducted a comparative study between five medicinal plants having the same geographic origin: the Hamadan region in the west of Iran and growing in the same natural conditions. The amount of total phenolics and total flavonoids for parts of these plants

Research paper thumbnail of Effect of methyl jasmonate and salicylic acid on noradrenalin accumulation in hairy roots of Portulaca oleracea L

Research paper thumbnail of Amelioration des techniques androgeniques par modification des conditions de croissance des plantes donneuses d'antheres chez Triticum aestivum L. em. Thell

Bulletin des Recherches Agronomiques de Gembloux, 1989

Research paper thumbnail of Molecular and morphological characterisation of <i>Ulva chaugulii, U. paschima</i> and <i>U. ohnoi</i> (Ulvophyceae) from the Persian Gulf, Iran

Botanica Marina, May 25, 2016

Species of Ulva are distributed worldwide in various aquatic habitats and their morphological cha... more Species of Ulva are distributed worldwide in various aquatic habitats and their morphological characters change considerably in response to environmental conditions, which makes identification in this genus problematic. All Ulva species so far described from the Persian Gulf, Iran, have been identified by classical taxonomic methods. In this study, Ulva spp. collected from the Persian Gulf were identified by combined morphological and molecular methods. We sequenced the internal transcribed spacer ITS2 and the rbcL gene in 15 specimens, representing typical morphotypes of over 50 samples. Maximum likelihood, maximum parsimony and Bayesian inference (BI) phylogenetic reconstructions using ITS and rbcL revealed clades, which we identified as Ulva chaugulii, U. paschima and U. ohnoi. These three species have not been recorded from the Persian Gulf, Iran before. The morphology of U. chaugulii differed considerably from its species description, showing further evidence for extreme intraspecific variation in morphology in this cosmopolitan genus.

Research paper thumbnail of In Vitro Belblet Formation of Mooseer (Allium Hirtifolium)

Research paper thumbnail of P.oleracea induced apoptosis and inhibited the cell growth in oral epithelial cancer cell line

Iranian Journal of Pharmacology and Therapeutics, Jun 10, 2019

Despite the progress in cancer therapies such as chemotherapy and radiation, its eradication rema... more Despite the progress in cancer therapies such as chemotherapy and radiation, its eradication remains as unattainable dream for the patients and doctors. Recently, using supplementary agents such as herbal medicine with fewer side effects seems efficient and attractive. Therefore, the purpose of the present study is to investigate if the ethanolic extract of P.oleracea has cytotoxicity and apoptosis induction on oral epithelial cancer cell line (KB cell line). The KB cells were cultured with different doses of ethanol extract (0

Research paper thumbnail of In Vitro Belblet Formation of Mooseer (Allium Hirtifolium)

Research paper thumbnail of Production and Realease of Tropane Alkaloids from Deadly Nighushade (Atropa Belladonna L.) in Hydroponic Condition

Research paper thumbnail of Anti-Proliferative Effects of Some Fractions of Elaeagnus Angustifolia L. Flower and Aerial Part of Nepeta Crispa L. On K562 Leukemic Cells

Research paper thumbnail of Cytotoxic Effects of Aqueous Extract of Portulaca oleracea on Oral Cancer Cell Line

Iranian Journal of Blood and Cancer, 2018

1Department of Molecular Genetics, Faculty of Sciences, Payam Noor University, Tehran, Iran 2Depa... more 1Department of Molecular Genetics, Faculty of Sciences, Payam Noor University, Tehran, Iran 2Departments of Biotechnology, Bu Ali Sina University, Hamadan, Iran 3Department of Biotechnology, Payam Noor University, Tehran, Iran 4Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran 5Medical Biotechnology Research Center, Paramedicine Faculty, Guilan University of Medical Sciences, Rasht, Iran

Research paper thumbnail of Investigating the in vitro cytotoxic effects of Cynara scolymus L

Research paper thumbnail of Evaluation of the Effect of Licorice Extract on Proliferation and Differentiation of Human Mesenchymal Stem Cells into Osteoblast cells

Research paper thumbnail of Dopamine Production in Hairy Root Cultures of Portulaca oleracea (Purslane) Using Agrobacterium rhizogenes

Journal of Agricultural Science and Technology, 2014

An efficient transformation system for the medicinal plant Portulaca oleracea was established usi... more An efficient transformation system for the medicinal plant Portulaca oleracea was established using agropine-type Agrobacterium rhizogenes ATCC15834 . Hairy roots were obtained directly from cotyledon leaves explants seven days after inoculation with the bacteria. The highest transformation efficiency was obtained from cotyledon leaves explants, and amounted to 53.3% within two weeks. Roots grew rapidly on solid growth regulator free ½ Murashige and Skoog medium and demonstrated characteristics of transformed roots such as fast growth and high lateral branching. Successful and stable transfer of rolB gene was illustrated by PCR using specific primers of the gene. The hairy roots showed an ability to synthesize natural and medicinal product, dopamine. Elicitation of dopamine production in P. oleracea hairy roots was tested using different concentrations of methyl jasmonate (0, 100, 150, 200 µM) and salicylic acid (0, 125, 250, 500 µM), added to the hairy root cultures during the late...

Research paper thumbnail of Agrobacterium-mediated transfer of -Glucoronidase gene (gusA) to water mint (Mentha aquatica L.)

Journal of Medicinal Plants Research, Mar 4, 2011

Water mint is a medicinal aromatic perennial herb belonging to the family Labiateae. It is distri... more Water mint is a medicinal aromatic perennial herb belonging to the family Labiateae. It is distributed mostly in the temperate and sub-temperate regions of the world. Water mint is valued for its multipurpose uses in the field of pharmaceuticals, cosmetics and flavoring foods. Agrobacteriummediated gene transformation has yielded a stunning array of transgenic plants with novel properties. Transgenic mint plants with reporter genes have been obtained only in peppermint. Therefore it would be interesting to extend transformation experiments to other mint species such as Water mint. Establishing a reliable direct regeneration system is necessary in plant transformation programs. In vitro direct regeneration of Water mint was obtained from leaf disks. Best results were obtained when explants were cultured onto MS medium supplemented with 4 mg/lit BAP. Transformed plants were obtained by co-cultivation of leaf disks with Agrobacterium tumefaciens strain GV3850 harbouring a binary vector pBI121 that carried E. coli-glucuronidase as reporter gene and nptII as selective marker gene for kanamycin resistance. The presence and expression of transgenes in transgenics was evaluated by GUS histoenzymatic assay and PCR. An overall transformation frequency of 12% was achieved.

Research paper thumbnail of Effects of natural extract of Rosmarinus officinalis L., Thymus vulgaris L. and benzyl adenine hormone on some quality traits and shelf-life of Polianthes tuberosa L. cut flowers

Iranian Journal of Medicinal and Aromatic Plants, 2018

Research paper thumbnail of Developing an efficient protocol for hairy root induction in Lythrum salicaria L

Research paper thumbnail of Adventitions shoot formation from leaf explants of oleaster (Elaeagnus angustifolia L.)

Propagation of Ornamental Plants, 2009

Department of �iote��nolog�, �a��lt� of �gri��lt�re, ��-�li �ina �ni�ersit�, �ama�an, �ran of �io... more Department of �iote��nolog�, �a��lt� of �gri��lt�re, ��-�li �ina �ni�ersit�, �ama�an, �ran of �iote��nolog�, �a��lt� of �gri��lt�re, ��-�li �ina �ni�ersit�, �ama�an, �ran of �iote��nolog�, �a��lt� of �gri��lt�re, ��-�li �ina �ni�ersit�, �ama�an, �ran �iote��nolog�, �a��lt� of �gri��lt�re, ��-�li �ina �ni�ersit�, �ama�an, �ran �iote��nolog�, �a��lt� of �gri��lt�re, ��-�li �ina �ni�ersit�, �ama�an, �ran , �a��lt� of �gri��lt�re, ��-�li �ina �ni�ersit�, �ama�an, �ran �a��lt� of �gri��lt�re, ��-�li �ina �ni�ersit�, �ama�an, �ran of �gri��lt�re, ��-�li �ina �ni�ersit�, �ama�an, �ran of �gri��lt�re, ��

Research paper thumbnail of Sixteen Year Analyses of Burned Areas in Iran Based on Provincial Borders and Land Use with the Aim of Prioritizing and Managing Fires: A Case Study

Research Square (Research Square), Feb 1, 2023

In the present research, the burned area of each province was investigated according to land use ... more In the present research, the burned area of each province was investigated according to land use in order to manage and prioritize provinces with extent and high-risk burns. The analyses of burned areas in Iran has been ful lled in Iran for the rst time. Following the expansion of agricultural lands around forests, the number of intentional wild res aiming at reducing forest area has increased. Only 5% of wild res in the Mediterranean region have a natural origin. In Iran, the nancial damage caused by wild res (apart from spiritual and intangible damages such as woods, bushes, and herbal plants) between 1968 and 2007 has been estimated at about 1.93 million dollars. Therefore, prioritizing and managing re in natural ecosystems seems to be essential. In the present study, the burned areas of Iran from 2002-03-21 to 2018-03-21 based on land use were obtained from the data available in the Global Wild re Information System and were categorized by provincial borders. In order to prepare Iran's land use map, related data (MCD12Q1) were obtained from https://www.earthdata.nasa.gov in HDF5 format. The results showed that Khuzestan, Fars, Kermanshah, and Golestan Provinces, respectively had the largest burned areas in the country. Considering that most burned areas of the country have been recorded in agricultural lands, human factors are very in uential in increasing the rate of burned areas, and maintaining distance between agricultural lands and native vegetation (fuel) is mandatory.

Research paper thumbnail of Antifungal Effects of Two Medicinal Plant Native to Iran

International journal of Advanced Biological and Biomedical Research, Apr 1, 2014

Objective: In order to use natural compounds in controlling plant pests and diseases, many resear... more Objective: In order to use natural compounds in controlling plant pests and diseases, many researchers in recent years have studied the antifungal effects of essential oils and plant extracts. The purpose of this study was to investigate the chemical composition and antifungal effects of Echium khuzestanium and marubbium anisodan extracts. Methods: After extraction and preparation of different concentrarion from extracts, antifungal effect on 4 plant fungi was stadied by disc diffusion method. chemical composition of plants was checked by GC/MS. Results: According to the results, 86 kinds of chemical compounds found in M.anisodan extract. Furfural, steroids, vitamin B and flavonoids are the main compounds of M.anisodan. 46 kinds of chemical compounds found in methanol extract of E.khuzestanium. there are mucilage, fatty acids, flavonoid and diterpenes in flower of E.khuzestanium.

Research paper thumbnail of Anticancer, antioxidant and antibacterial activities of extracts from Scrophularia striata and Elaeagnus angustifolia, growing in Ilam and Kurdistan provinces in Iran

Plant science today, Nov 9, 2022

Emerging evidence of the impact of plant compounds on growth inhibition of micropathogens and can... more Emerging evidence of the impact of plant compounds on growth inhibition of micropathogens and cancer cells has opened new areas to evaluate plants' treatment properties. Here, we aimed to investigate in vitro antioxidant, antibacterial and anticancer effects of the secondary metabolites isolated from different extracts produced by Elaeagnus angustifolia and Scrophularia striata. Antibacterial activity was evaluated against human and plant pathogenic bacteria by 3 methods of tubular dilution, well and disc diffusion. The anticancer effect of E. angustifolia extract was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Biochemical experiments showed the presence of compounds such as phenol, flavonoids, resins, quinones, steroids, terpenoids and alkaloids in extracts, with the highest antioxidant activity of the methanolic extracts. For both plants' strains, the disc method was more effective than the well diffusion method. The highest yields were obtained from Methanolic, ethanolic and hydroalcoholic extracts for E. angustifolia and aqueous extract for Scrophularia striata. The most sensitive bacteria for E. angustifolia were Bacillus subtilis and Xanthomonas campestris against pit extracts and Clavibacter michiganensis against pulp extracts. The most sensitive bacteria for S. striata were Bacillus subtilis and Staphylococcus aureus. Methanolic and aqueous solvents showed the maximum bacterial inhibitory and bactericidal activities in the Minimum inhibitory concentration (MIC) and the minimum bactericidal concentration (MBC) tests respectively. Additionally, E. angustifolia showed anticancer effects toward MCF7 breast cancer cells. These findings provided a better understanding of the widespread application of these plants as potential antioxidants, antibacterial and anticancer sources, and safe natural medicines in health maintenance and disease treatment.

Research paper thumbnail of Comparative evaluation of the antioxidant potential of some Iranian medicinal plants

Food Chemistry, 2007

Medicinal plants are a source for a wide variety of natural antioxidants. In the study reported h... more Medicinal plants are a source for a wide variety of natural antioxidants. In the study reported here, we have conducted a comparative study between five medicinal plants having the same geographic origin: the Hamadan region in the west of Iran and growing in the same natural conditions. The amount of total phenolics and total flavonoids for parts of these plants

Research paper thumbnail of Effect of methyl jasmonate and salicylic acid on noradrenalin accumulation in hairy roots of Portulaca oleracea L

Research paper thumbnail of Amelioration des techniques androgeniques par modification des conditions de croissance des plantes donneuses d'antheres chez Triticum aestivum L. em. Thell

Bulletin des Recherches Agronomiques de Gembloux, 1989

Research paper thumbnail of Molecular and morphological characterisation of <i>Ulva chaugulii, U. paschima</i> and <i>U. ohnoi</i> (Ulvophyceae) from the Persian Gulf, Iran

Botanica Marina, May 25, 2016

Species of Ulva are distributed worldwide in various aquatic habitats and their morphological cha... more Species of Ulva are distributed worldwide in various aquatic habitats and their morphological characters change considerably in response to environmental conditions, which makes identification in this genus problematic. All Ulva species so far described from the Persian Gulf, Iran, have been identified by classical taxonomic methods. In this study, Ulva spp. collected from the Persian Gulf were identified by combined morphological and molecular methods. We sequenced the internal transcribed spacer ITS2 and the rbcL gene in 15 specimens, representing typical morphotypes of over 50 samples. Maximum likelihood, maximum parsimony and Bayesian inference (BI) phylogenetic reconstructions using ITS and rbcL revealed clades, which we identified as Ulva chaugulii, U. paschima and U. ohnoi. These three species have not been recorded from the Persian Gulf, Iran before. The morphology of U. chaugulii differed considerably from its species description, showing further evidence for extreme intraspecific variation in morphology in this cosmopolitan genus.

Research paper thumbnail of In Vitro Belblet Formation of Mooseer (Allium Hirtifolium)

Research paper thumbnail of P.oleracea induced apoptosis and inhibited the cell growth in oral epithelial cancer cell line

Iranian Journal of Pharmacology and Therapeutics, Jun 10, 2019

Despite the progress in cancer therapies such as chemotherapy and radiation, its eradication rema... more Despite the progress in cancer therapies such as chemotherapy and radiation, its eradication remains as unattainable dream for the patients and doctors. Recently, using supplementary agents such as herbal medicine with fewer side effects seems efficient and attractive. Therefore, the purpose of the present study is to investigate if the ethanolic extract of P.oleracea has cytotoxicity and apoptosis induction on oral epithelial cancer cell line (KB cell line). The KB cells were cultured with different doses of ethanol extract (0

Research paper thumbnail of In Vitro Belblet Formation of Mooseer (Allium Hirtifolium)

Research paper thumbnail of Production and Realease of Tropane Alkaloids from Deadly Nighushade (Atropa Belladonna L.) in Hydroponic Condition

Research paper thumbnail of Anti-Proliferative Effects of Some Fractions of Elaeagnus Angustifolia L. Flower and Aerial Part of Nepeta Crispa L. On K562 Leukemic Cells

Research paper thumbnail of Cytotoxic Effects of Aqueous Extract of Portulaca oleracea on Oral Cancer Cell Line

Iranian Journal of Blood and Cancer, 2018

1Department of Molecular Genetics, Faculty of Sciences, Payam Noor University, Tehran, Iran 2Depa... more 1Department of Molecular Genetics, Faculty of Sciences, Payam Noor University, Tehran, Iran 2Departments of Biotechnology, Bu Ali Sina University, Hamadan, Iran 3Department of Biotechnology, Payam Noor University, Tehran, Iran 4Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran 5Medical Biotechnology Research Center, Paramedicine Faculty, Guilan University of Medical Sciences, Rasht, Iran

Research paper thumbnail of Investigating the in vitro cytotoxic effects of Cynara scolymus L

Research paper thumbnail of Evaluation of the Effect of Licorice Extract on Proliferation and Differentiation of Human Mesenchymal Stem Cells into Osteoblast cells

Research paper thumbnail of Dopamine Production in Hairy Root Cultures of Portulaca oleracea (Purslane) Using Agrobacterium rhizogenes

Journal of Agricultural Science and Technology, 2014

An efficient transformation system for the medicinal plant Portulaca oleracea was established usi... more An efficient transformation system for the medicinal plant Portulaca oleracea was established using agropine-type Agrobacterium rhizogenes ATCC15834 . Hairy roots were obtained directly from cotyledon leaves explants seven days after inoculation with the bacteria. The highest transformation efficiency was obtained from cotyledon leaves explants, and amounted to 53.3% within two weeks. Roots grew rapidly on solid growth regulator free ½ Murashige and Skoog medium and demonstrated characteristics of transformed roots such as fast growth and high lateral branching. Successful and stable transfer of rolB gene was illustrated by PCR using specific primers of the gene. The hairy roots showed an ability to synthesize natural and medicinal product, dopamine. Elicitation of dopamine production in P. oleracea hairy roots was tested using different concentrations of methyl jasmonate (0, 100, 150, 200 µM) and salicylic acid (0, 125, 250, 500 µM), added to the hairy root cultures during the late...

Research paper thumbnail of Agrobacterium-mediated transfer of -Glucoronidase gene (gusA) to water mint (Mentha aquatica L.)

Journal of Medicinal Plants Research, Mar 4, 2011

Water mint is a medicinal aromatic perennial herb belonging to the family Labiateae. It is distri... more Water mint is a medicinal aromatic perennial herb belonging to the family Labiateae. It is distributed mostly in the temperate and sub-temperate regions of the world. Water mint is valued for its multipurpose uses in the field of pharmaceuticals, cosmetics and flavoring foods. Agrobacteriummediated gene transformation has yielded a stunning array of transgenic plants with novel properties. Transgenic mint plants with reporter genes have been obtained only in peppermint. Therefore it would be interesting to extend transformation experiments to other mint species such as Water mint. Establishing a reliable direct regeneration system is necessary in plant transformation programs. In vitro direct regeneration of Water mint was obtained from leaf disks. Best results were obtained when explants were cultured onto MS medium supplemented with 4 mg/lit BAP. Transformed plants were obtained by co-cultivation of leaf disks with Agrobacterium tumefaciens strain GV3850 harbouring a binary vector pBI121 that carried E. coli-glucuronidase as reporter gene and nptII as selective marker gene for kanamycin resistance. The presence and expression of transgenes in transgenics was evaluated by GUS histoenzymatic assay and PCR. An overall transformation frequency of 12% was achieved.