Khosro Sardarian - Academia.edu (original) (raw)

Papers by Khosro Sardarian

Journal of research in health sciences, May 31, 2014

Background: Previous studies revealed that selectins play key roles in homing of immune cells to ... more Background: Previous studies revealed that selectins play key roles in homing of immune cells to inflamed tissues and lymphatic organs. L-selectins are expressed on immune cells and interact with P and E selectins to homing to the tissues, hence, the polymorphisms within the gene of L-selectins may are associated with alteration in its expression. Thus, the current cross-sectional analytical study has been designed to investigate the polymorphisms within L-selectin gene and their relation with visceral leishmaniasis (VL). Methods: This study was performed on 194 samples during 2004-2012.The PCR-SSP and immunoflorescence techniques were used to evaluate the L-selectins polymorphism and anti-Leishmania antibody titration, respectively, in 56, 74 and 64 seropositive VL patients (group 1), seropositive healthy controls (group 2) and seronegative healthy controls (group 3). Results: The results showed that the genotypes (P=0.711) and alleles (P=0.679) within Lselectins gene (A/C) was not differ between groups. Our results also demonstrated that the genotypes within L-selectins in group 1 (P=0.807) and 2 (P=0.441) were not associated with the titration of anti-leishmania antibody. Conclusions: The results identified that the polymorphisms within L-selectins gene were not associated with VL and it may be concluded that these genotypes and alleles are unable to affect immune responses in VL patients.

Journal of Guilan University of Medical Sciences, 2002

Context: Ascaris Lumbericoids infection is more common in children and teenagers and in areas wit... more Context: Ascaris Lumbericoids infection is more common in children and teenagers and in areas with poor sanitation or crowded living conditions. Largest of all the parasites inhabiting the human intestine it is also the most common of parasitic infections in developing countries. It has been reported to infect about one-fourth of the world population. Chronic ascariasis has been reported to adversely effect the growth rate and anthropometric indices in infected individuals of growing age. Aims: The goal of this research was to evaluate the anthropometric indices in individuals infected with Ascaris in Hamedan province of Iran. Settings and Design: This was a descriptive cross sectional study. Methods and Material: Six hundred fourteen randomly selected individuals who were inhabitants of Hamedan province of Iran were chosen for this study. Stool samples of these individuals were collected and then experimented with Formel-Ether method to determine Ascaris Lumbericoids infection. Hei...

Scientific Journal of Hamadan University of Medical Sciences, 2001

Intestinal protozoa and helminths are aetiological agents of several widespread para... more Intestinal protozoa and helminths are aetiological agents of several widespread parasitic diseases. The most common ones are Entamoeba histolytica , Giardia lamblia and Ascaris lumbricoides. The main purposes of this study were determination the frequency of intestinal parasites,and study the clinical symptoms of patients and also to indicate the correlation between symptoms and parasitic infection and the kind of parasites. Two hundreds and twenty seven patients were examined by physicians and directed to parasitology laboratory for stool examination by direct method and formol - ether (concentration method ). The demographic characteristic and the gasterointestinal symptoms of patients were provided through questionaire. Frequency of parasitic helminths and protozoa were 20.2% and 22.9% respectively. However 42.6% of the patients were infected by at least one pathogenic parasites . The most infected cases had G.lamblia (19.3%),and the most common symptom was abdomen pain and the least one was vomiting. In the Ascaris infected cases, the most common symptom was vomiting (57.1%) and in the Giardia infected cases, the most common symptom was abdomen pain (64.7%). Ascaris and Giardia had the highest prevalence among the studied patients. The prevalence of pathogenic intestinal parasites in study group who had gastrointestinal symptoms was high (42.6%). The main symptoms were the same as those usually observed in patients infected with intestinal parasites. Some of non-pathogenic diseases may also cause similar symptoms.

Medical Laboratory Journal, 2007

Background and aims: Previous studies have shown that the intestinal parasites have the high prev... more Background and aims: Previous studies have shown that the intestinal parasites have the high prevalence in Hamadan province. However, this problem has not been addressed properly yet and, therefore, there is not up-to-date information about it. This study aimed to determine the prevalence of intestinal parasites in different residential areas of Hamadan Province (urban and rural areas) and compare the obtained findings with the results from previous studies on these parasites in this region and other parts of Iran. Methods: In this cross-sectional study, a total of 2948 fecal samples were collected from all rural and urban inhabitants of the province (including 12 cities) by cluster random sampling method. Fecal samples were examined using the direct smear and formalin ether concentration techniques. A questionnaire was used to collect relevant epidemiologic information about the study population. Results: Overall, 52.2% of the samples were from rural areas, and the rest were from u...

The International Journal of Occupational and Environmental Medicine, 2020

Background: Extremely low-frequency electromagnetic fields (ELF-EMFs) are abundantly produced in ... more Background: Extremely low-frequency electromagnetic fields (ELF-EMFs) are abundantly produced in modern societies. In recent years, interest in the possible effects of ELF-EMFs on the immune system has progressively increased. Objective: To examine the effects of ELF-EMFs with magnetic flux densities of 1, 100, 500, and 2000 µT on the serum levels of interleukin (IL)-9, IL-10, and tumor necrosis factor-alpha (TNF-α). Methods: 80 adult male rats were exposed to ELF-EMFs at a frequency of 50 Hz for 2 h/day for 60 days. The serum cytokines were measured at two phases of pre- and post-stimulation of the immune system by human serum albumin (HSA). Results: Serum levels of IL-9 and TNF-α, as pro-inflammatory cytokines, were decreased due to 50 Hz EMFs exposure compared with the controls in the pre- and post-stimulation phases. On the contrary, exposures to 1 and 100 µT 50 Hz EMFs increased the levels of antiinflammatory cytokine, and IL-10 only in the pre-stimulation phase. In the post-st...

Electromagnetic Biology and Medicine, 2019

ABSTRACT The study investigated the effect of extremely low-frequency electromagnetic fields (ELF... more ABSTRACT The study investigated the effect of extremely low-frequency electromagnetic fields (ELF-EMFs) exposure at different magnetic flux densities on genes expression of transcription factor Maf (c-Maf), signal transducer and activator of transcription 6 (STAT6), and retinoid-related orphan receptor alpha (RORα) in the spleen and thymus of rats. Eighty adult male rats were separated into four ELF-EMFs exposed and were exposed to magnetic flux densities of 1, 100, 500, and 2000 µT at a frequency of 50 Hz for 2 h daily for up to 60 d. All rats were intraperitoneally immunized on d 31, 44, and 58 of exposure. The experimental results showed that the expression levels of c-Maf, STAT6, and RORα in the thymus were not significantly changed at different magnetic flux densities. The expression levels of RORα and c-Maf were significantly downregulated at the densities of 1 and 100 µT, while the expression of STAT6 was only significantly decreased at the density of 100 µT. In conclusion, low magnetic flux densities of ELF-EMFs may reduce the expression levels of c-Maf, STAT6, and RORα genes in the spleen.

Clinical Laboratory, 2019

BACKGROUND The onset of acute toxoplasmosis in pregnant women may pose a risk to their growing fe... more BACKGROUND The onset of acute toxoplasmosis in pregnant women may pose a risk to their growing fetuses. The timely diagnosis of infection in managing the disease and preventing its harmful consequences on the fetus is very important. Therefore, the study was conducted to identify acute toxoplasmosis in the pregnant women by detecting the specific IgM antibody and Toxoplasma gondii B1 gene. METHODS A total of 653 serum samples of women who attended to Fatemieh Hospital of Hamadan University of Medical Sciences were tested for IgG antibodies against Toxoplasma gondii by enzyme-linked immunosorbent as-say (ELISA). The IgG positive specimens were further examined for IgM by ELISA and polymerase chain reaction (PCR) for B1 gene. In the second phase, change in IgG titers was evaluated on 47 IgG positive samples after two weeks. RESULTS ELISA data showed that 167 out of 653 and 2 out of 167 samples were positive for IgG (25.6%) and IgM (1.2%), respectively. However, PCR detection showed that 36 cases (21.6%) were positive for the B1 gene. Seven out of 47 IgG positive samples showed an increase in the antibody titer and positive for the B1 gene. The most cases of IgG positives and the B1 gene samples were associated with the third trimester of pregnancy with 49.7% and 14%, respectively, and the most common abundance of the B1 gene was 14.4% in the age group of 26 - 35. The most commonly reported clinical symptoms in the B1 gene-positive women were nausea 15 (41.7%), cough 13 (36.1%), headache 12 (33.3%), and vomiting 11 (30.5%). CONCLUSIONS Using PCR and the B1 gene in serum samples of pregnant women to detect acute toxoplasmosis is a more appropriate and accurate method than IgM antibody.

Human antibodies, Jan 14, 2018

Congenital toxoplasmosis is an important disease that occurs when pregnant women become infected ... more Congenital toxoplasmosis is an important disease that occurs when pregnant women become infected with Toxoplasma gondii during pregnancy. The aim of this study was to investigate the presence of T. gondii B1 gene in placental tissues of IgM seronegative women. In this research, chronic toxoplasmosis was identified through examination of blood samples in a group of pregnant women by anti-Toxoplasma IgG and IgM ELISA and nested-PCR techniques. IgG avidity test was used to estimate the onset of infection in some pregnant women with chronic infection. After delivery, some umbilical cord and neonatal blood were tested by anti-Toxoplasma IgM ELISA, and also the B1 gene of T. gondii was investigated in their placental tissue by nested-PCR. Some factors such as blood sampling time and some clinical symptoms experienced during pregnancy were recorded. One hundred and sixty seven out of 653 (25.6%) pregnant women were positive for anti-Toxoplasma IgG. Of them, 165 (98.8%) were negative for an...

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Advanced biomedical research, 2018

One of the consequences of toxoplasmosis is the risk of passing it from mother to fetus and the o... more One of the consequences of toxoplasmosis is the risk of passing it from mother to fetus and the onset of congenital toxoplasmosis during pregnancy. The purpose of this study was to evaluate the B1 gene of in the placental tissues of pregnant women with acute toxoplasmosis. The study was a cross-sectional study. Serum samples of pregnant women who attended to Fatemieh Hospital of Hamadan University of Medical Sciences were tested for immunoglobulin G (IgG) antibodies against by enzyme-linked immunosorbent assay. Then, polymerase chain reaction was used to identify the specific B1 gene of in IgG seropositive women. The placental tissues of the pregnant women with positive serum B1 gene examined for this gene. Anti- immunoglobulin M (IgM) was performed on the umbilical cord and neonate blood. Anti- IgG was detected in 167 out of 653 (25.6%) pregnant women. B1 gene was identified in 36 out of 167 (21.6%) of IgG seropositive women. After delivery, the B1 gene was evaluated in 15 out of 3...

Asian Pacific journal of tropical medicine, Nov 1, 2016

To evaluated the relationship between the genetic variations at IL-8 +2767 position with VL patho... more To evaluated the relationship between the genetic variations at IL-8 +2767 position with VL pathogenesis among Iranian patients. Three groups including patients with VL clinical presentation and leishmania seropositive (n = 124), patients seropositive but without clinical presentation (n = 82) and healthy controls (n = 63) were selected to conduct this cross-sectional study. Polymorphism at +2767 position of IL-8 was investigated using PCR-RFLP techniques. Anti-leishmania antibody titration was evaluated by the immunoflorescence technique. We observed higher significant frequencies +2767 A/A and A/T genotypes in Group 1 compared to Group 2 and healthy controls (P = 0.001). Also, patients in Group 1 carrying A/A genotype showed higher titer of anti-leishmania antibody than patients with A/T and T/T genotypes (P = 0.05). The validity of the data was analyzed using Hardy-Weinberg equilibrium and one way analysis of variance (ANOVA), as well as χ(2) tests. Our findings indicate that the...

Advanced biomedical research, 2015

Immune responses play significant roles in protection against leishmaniasis. Polymorphisms within... more Immune responses play significant roles in protection against leishmaniasis. Polymorphisms within the interleukin 4 receptor alpha chain (IL-4Rα) gene affect the production of cytokines, which is important for the clearance of many pathogens. The aim of the current study was to identify the relationship between visceral leishmaniasis (VL) infection and polymorphisms at positions T1432C and A1652G of IL-4Rα in an Iranian population. This cross-sectional study was performed during 2004-2012 and included three groups of participants: VL patients (Group 1, n = 124), seropositive healthy controls (Group 2, n = 101), and seronegative healthy controls (Group 3, n = 55). The IL-4Rα T1432C and A1652G polymorphisms were evaluated using a polymerase chain reaction-restriction fragment length polymorphism technique, and anti-Leishmania antibody titers were determined by using immunofluorescence technique. Alleles and genotypes were compared between groups of the study as well as Groups 1 and 2 ...

Jundishapur Journal of Microbiology, 2012

Blastocystis hominis is a common protozoan parasite in the human and many other animals intestine... more Blastocystis hominis is a common protozoan parasite in the human and many other animals intestine. The parasite pathogenicity is still controversial. A few studies have been conducted on prevalence of B. hominis subtypes in Iran. The results of present study improve our knowledge on B. hominis subtypes in Iran and therefore we can do more investigations on pathogenicity potential of these subtypes in the future. Background: Blastocystis is a common protozoan parasite in mammals, birds, amphibians, reptiles, fish, arthropods, and annelids. This parasite has some subtypes, which pathogenicity status of them still remained controversial. Some of Blastocystis subtypes are potentially pathogenic to human. Objectives: This study has identified the Blastocystis hominis subtypes and their prevalence rates in Hamadan. Materials and Methods: During two months of summer 2011, a total number of 250 human fecal samples referred for parasitology examination to Beasat Hospital and a few clinical laboratories of Hamadan city were collected. The samples were examined by direct method and formalin-ether. 41 samples exhibited positive results for B. hominis thereby were cultured in Locke-egg medium. After the growth and in order to genotype identification, B. hominis isolates were amplified by PCR, using seven pairs of sequencestagged site primers. Results: In this study, three subtypes of B. hominis consisted of one [SB83], two [SB340] and three [SB227] were identified. The most dominant genotype was SB83 with 56.1 % frequency. The prevalence rate of genotype SB227 and SB340 were 22 % and 7.3 %, respectively. Coexistence of genotypes SB83 and SB227 was detected in 14.6 % of positive cases. Conclusions: This is the first study in Hamadan on genotyping of B. hominis, which may trigger other epidemiologic and zoonotic studies on different subtypes and hence control clinical manifestations of infection.

Inflammation, 2013

Previous investigations demonstrated that immune responses play critical roles in the defense aga... more Previous investigations demonstrated that immune responses play critical roles in the defense against visceral leishmaniasis (VL). A key regulator of immune responses is the cytokine, IL-10 and polymorphisms within its promoter which could alter its expression. Thus, the aim of this study was to examine the correlation between polymorphism at the -819 position of the IL-10 gene and VL in a selected Iranian population. This cross-sectional study was performed on 100 patients with clinical presentation of VL and seropositive for the leishmania (group 1), 62 patients without clinical presentation but seropositive (group 2), and 128 healthy controls (group 3). The IL-10 -819 polymorphism was evaluated using the PCR-RFLP technique. The anti-leishmania antibody titration was assessed using an immunofluorescence assay. Our results showed that the polymorphism at IL-10 -819 (C/T) position was significantly associated with VL, and C/T genotype was significantly higher in VL patients when compared to groups 2 and 3 (p &amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;lt; 0.001). However, the results demonstrated that the C and T alleles were not associated with VL (p = 0.855). The data presented here confirm the results of previous reports that polymorphisms at the -819 position of the IL-10 gene can influence susceptibility to VL suggesting that the C/T genotype may be considered as a risk factor for the disease.

Preventive Veterinary Medicine, 2013

Certain zoonotic parasites are enteropathogens in dogs that cause serious human disease such as c... more Certain zoonotic parasites are enteropathogens in dogs that cause serious human disease such as cystic echinococcosis, human alveolar echinococcosis, visceral larva migrans, and ocular larva migrans. This study investigated the prevalence of intestinal parasites in dogs in the Chenaran County, Razavi Khorasan Province, Iran. Sampling was carried out randomly in 17 villages from November 2009 to January 2010. Seventy-seven fecal samples from 28 domestic and 49 stray dogs were examined using sieving/flotation and modified Ziehl-Neelsen staining. Intestinal parasites were found in 51 of the 77 (66%) dogs most common being Toxascaris leonina (29%, 22/77), Toxocara spp. (25%, 19/77), Eimeria spp. (19%, 15/77), Taenia/Echinococcus spp. (18%, 14/77), Sarcocystis spp. (17%, 13/77), and Dicrocoelium dendriticum (14%, 11/77). Lower infection rates of parasites were observed for Trichuris vulpis (6%, 5/77), Cryptosporidium spp. (5%, 4/77), and Physaloptera spp. (3%, 2/77). Prevalence of infection by Dipylidium caninum, Capillaria spp., Cystoisospora spp., and hookworms was similar (1%, 1/77). This study is the first report of the prevalence of intestinal parasites of domestic and stray dogs in Chenaran County, Northeast Iran. The higher prevalence of zoonotic intestinal parasites such as Toxascaris leonina, Toxocara spp. and Taenia/Echinococcus spp. compared to other parasites indicates the need for control programs to minimize the risk of transmission of zoonotic disease, particularly cystic echinococcosis, alveolar echinococcosis, visceral larva migrans, and ocular larva migrans to people living in these areas.