Kozeta Filipi - Academia.edu (original) (raw)

Papers by Kozeta Filipi

Research paper thumbnail of Assessment of cervical cytological data in Albanian females

Asian Pacific Journal of Cancer Prevention, 2014

Cervical cancer is one of the most common female malignancies with high mortality rates in develo... more Cervical cancer is one of the most common female malignancies with high mortality rates in developing countries. Our purpose was to determine the prevalence of cervical cytological abnormalities by cervical cytology (CC) and the analysis of risk factors in Albanian population. A total of 5,416 conventional pap smear tests collected between January 2009 and January 2012 from Tirana University Hospital Obstetrics-Gynecology "Queen Geraldine" were retrospectively analyzed. A total of 258 (4.8%) cases had epithelial abnormalities. The numbers and rates were as follows: atypical squamous cell of undetermined significance (ASCUS; n=150 [2.76%]); atypical glandular cells of undetermined significance (AGUS; n=8 [0.14%]); low-grade squamous intraepithelial lesion (LSIL; n=87 [1.6%]); high- grade squamous intraepithelial lesion (HSIL; n=10 [0.18%]); and squamous cell carcinoma (SCC; n=3 [0.05%]). The prevalence of cervical cytological abnormality in our study was 4.8%. A larger comm...

Research paper thumbnail of Neoplastic Tissues

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Research paper thumbnail of Patients' pain evaluation after cardiac surgery intervention: correlation between the type of graft used, group ages and gender

Research paper thumbnail of Assessment of Cervical Cytological Data in Albanian Females

Asian Pacific Journal of Cancer Prevention, 2014

Research paper thumbnail of Risk Factors Assessment in Acute Renal Dysfunction Following Cardiac Surgery in Tirana, Albania

International Journal of Scientific Research, 2012

Background. Acute kidney dysfunction (ARD) is a serious complication after cardiac surgery. It ma... more Background. Acute kidney dysfunction (ARD) is a serious complication after cardiac surgery. It may require the use of renal replacement therapy. Many risk factors and algorithms are found. Methods. Data from 72 cardiosurgery patients were collected. Age, diabetes mellitus, clamp and pump time, operation type, were studied. The patients were categorized into group 1: Patients with post operative normal kidney function and group 2: Patients with post operative ARD. All patients were followed with measurement serum creatinine post-operatively. Results. ARD incidence was 13.9%. Mean age in group 1 was 60.95±9.52 and in group 2 was 63.00±10.7 years. There was not any sig- nificant correlation between duration of clamp time and post operation acute renal dysfunction. Fifteen patients (93.75%) of group 1 and one (6.25%) in group 2 were diabetic. Conclusion. Acurate scoring algorithms should be available for prediction of post-cardiac surgery complications.

Research paper thumbnail of Oxidative stress in HPV-driven viral carcinogenesis: redox proteomics analysis of HPV-16 dysplastic and neoplastic tissues

PloS one, 2012

Genital infection by high risk Human Papillomavirus (HR-HPV), although recognized as the main eti... more Genital infection by high risk Human Papillomavirus (HR-HPV), although recognized as the main etio-pathogenetic factor of cervical cancer, is not per se sufficient to induce tumour development. Oxidative stress (OS) represents an interesting and under-explored candidate as a promoting factor in HPV-initiated carcinogenesis. To gain insight into the role of OS in cervical cancer, HPV-16 positive tissues were collected from patients with invasive squamous cervical carcinoma, from patients with High Grade dysplastic HPV lesions and from patients with no clinical evidence of HPV lesions. After virological characterization, modulation of proteins involved in the redox status regulation was investigated. ERp57 and GST were sharply elevated in dysplastic and neoplastic tissues. TrxR2 peaked in dysplastic samples while iNOS was progressively reduced in dysplastic and neoplastic samples. By redox proteomic approach, five proteins were found to have increased levels of carbonyls in dysplastic...

Research paper thumbnail of Prevalence of high-risk human papillomavirus and distribution of HPV16/18/45 types in Albanian young women before HPV vaccination

Aim: To estimate the prevalence of high-risk human papillomavirus (hr-HPV) infection among 18-24 ... more Aim: To estimate the prevalence of high-risk human papillomavirus (hr-HPV) infection among 18-24 year old women, prior to implementation of prophylactic HPV vaccination in Albania, and to determine 16/18/45 HPV genotypes distribution in order to assess the potential benefit of HPV vaccination. Methods: A total of 382 women, aged 18-24 years, were screened with Digene Hybrid Capture 2 HPV DNA Test for 13 hr-HPV types at the Molecular Biology Laboratory of the national Institute of Public Health in Tirana, Albania. Genotyping for hr-HPV 16/18/45 types was performed to positive women. Results: Out of 382 samples, 95 (24.87%) [95% CI, 19.87-29.87] were hr-HPV positive. The prevalence of infection with HPV 16 was 21.05% (95%CI=16.0%-26.1%), whereas the prevalence of infection with HPV 18 was 10.52% (95%CI=5.5%-15.5%). The hr-HPV prevalence in women with abnormal cytology was 27.4%; nonetheless, it was no significantly higher than in women with normal cytology (57.9%). Conclusion: The hr-...

Research paper thumbnail of Genital human papillomavirus infection and genotype prevalence among albanian women: A cross-sectional study

Research paper thumbnail of Genital human papillomavirus infection and genotype prevalence among albanian women: A cross-sectional study

Journal of Medical Virology, 2000

Research paper thumbnail of Oxidative Stress in HPV-Driven Viral Carcinogenesis: Redox Proteomics Analysis of HPV-16 Dysplastic and Neoplastic Tissues

Genital infection by high risk Human Papillomavirus (HR-HPV), although recognized as the main eti... more Genital infection by high risk Human Papillomavirus (HR-HPV), although recognized as the main etio-pathogenetic factor of
cervical cancer, is not per se sufficient to induce tumour development. Oxidative stress (OS) represents an interesting and
under-explored candidate as a promoting factor in HPV-initiated carcinogenesis. To gain insight into the role of OS in
cervical cancer, HPV-16 positive tissues were collected from patients with invasive squamous cervical carcinoma, from
patients with High Grade dysplastic HPV lesions and from patients with no clinical evidence of HPV lesions. After virological
characterization, modulation of proteins involved in the redox status regulation was investigated. ERp57 and GST were
sharply elevated in dysplastic and neoplastic tissues. TrxR2 peaked in dysplastic samples while iNOS was progressively
reduced in dysplastic and neoplastic samples. By redox proteomic approach, five proteins were found to have increased
levels of carbonyls in dysplastic samples respect to controls namely: cytokeratin 6, actin, cornulin, retinal dehydrogenase
and GAPDH. In carcinoma samples the peptidyl-prolyl cis-trans isomerase A, ERp57, serpin B3, Annexin 2 and GAPDH were
found less oxidized than in dysplastic tissues. HPV16 neoplastic progression seems associated with increased oxidant
environment. In dysplastic tissues the oxidative modification of DNA and proteins involved in cell morphogenesis and
terminal differentiation may provide the conditions for the neoplastic progression. Conversely cancer tissues seem to attain
an improved control on oxidative damage as shown by the selective reduction of carbonyl adducts on key detoxifying/prosurvival
proteins

Research paper thumbnail of Assessment of cervical cytological data in Albanian females

Asian Pacific Journal of Cancer Prevention, 2014

Cervical cancer is one of the most common female malignancies with high mortality rates in develo... more Cervical cancer is one of the most common female malignancies with high mortality rates in developing countries. Our purpose was to determine the prevalence of cervical cytological abnormalities by cervical cytology (CC) and the analysis of risk factors in Albanian population. A total of 5,416 conventional pap smear tests collected between January 2009 and January 2012 from Tirana University Hospital Obstetrics-Gynecology "Queen Geraldine" were retrospectively analyzed. A total of 258 (4.8%) cases had epithelial abnormalities. The numbers and rates were as follows: atypical squamous cell of undetermined significance (ASCUS; n=150 [2.76%]); atypical glandular cells of undetermined significance (AGUS; n=8 [0.14%]); low-grade squamous intraepithelial lesion (LSIL; n=87 [1.6%]); high- grade squamous intraepithelial lesion (HSIL; n=10 [0.18%]); and squamous cell carcinoma (SCC; n=3 [0.05%]). The prevalence of cervical cytological abnormality in our study was 4.8%. A larger comm...

Research paper thumbnail of Neoplastic Tissues

See next page for additional authors Follow this and additional works at:

Research paper thumbnail of Patients' pain evaluation after cardiac surgery intervention: correlation between the type of graft used, group ages and gender

Research paper thumbnail of Assessment of Cervical Cytological Data in Albanian Females

Asian Pacific Journal of Cancer Prevention, 2014

Research paper thumbnail of Risk Factors Assessment in Acute Renal Dysfunction Following Cardiac Surgery in Tirana, Albania

International Journal of Scientific Research, 2012

Background. Acute kidney dysfunction (ARD) is a serious complication after cardiac surgery. It ma... more Background. Acute kidney dysfunction (ARD) is a serious complication after cardiac surgery. It may require the use of renal replacement therapy. Many risk factors and algorithms are found. Methods. Data from 72 cardiosurgery patients were collected. Age, diabetes mellitus, clamp and pump time, operation type, were studied. The patients were categorized into group 1: Patients with post operative normal kidney function and group 2: Patients with post operative ARD. All patients were followed with measurement serum creatinine post-operatively. Results. ARD incidence was 13.9%. Mean age in group 1 was 60.95±9.52 and in group 2 was 63.00±10.7 years. There was not any sig- nificant correlation between duration of clamp time and post operation acute renal dysfunction. Fifteen patients (93.75%) of group 1 and one (6.25%) in group 2 were diabetic. Conclusion. Acurate scoring algorithms should be available for prediction of post-cardiac surgery complications.

Research paper thumbnail of Oxidative stress in HPV-driven viral carcinogenesis: redox proteomics analysis of HPV-16 dysplastic and neoplastic tissues

PloS one, 2012

Genital infection by high risk Human Papillomavirus (HR-HPV), although recognized as the main eti... more Genital infection by high risk Human Papillomavirus (HR-HPV), although recognized as the main etio-pathogenetic factor of cervical cancer, is not per se sufficient to induce tumour development. Oxidative stress (OS) represents an interesting and under-explored candidate as a promoting factor in HPV-initiated carcinogenesis. To gain insight into the role of OS in cervical cancer, HPV-16 positive tissues were collected from patients with invasive squamous cervical carcinoma, from patients with High Grade dysplastic HPV lesions and from patients with no clinical evidence of HPV lesions. After virological characterization, modulation of proteins involved in the redox status regulation was investigated. ERp57 and GST were sharply elevated in dysplastic and neoplastic tissues. TrxR2 peaked in dysplastic samples while iNOS was progressively reduced in dysplastic and neoplastic samples. By redox proteomic approach, five proteins were found to have increased levels of carbonyls in dysplastic...

Research paper thumbnail of Prevalence of high-risk human papillomavirus and distribution of HPV16/18/45 types in Albanian young women before HPV vaccination

Aim: To estimate the prevalence of high-risk human papillomavirus (hr-HPV) infection among 18-24 ... more Aim: To estimate the prevalence of high-risk human papillomavirus (hr-HPV) infection among 18-24 year old women, prior to implementation of prophylactic HPV vaccination in Albania, and to determine 16/18/45 HPV genotypes distribution in order to assess the potential benefit of HPV vaccination. Methods: A total of 382 women, aged 18-24 years, were screened with Digene Hybrid Capture 2 HPV DNA Test for 13 hr-HPV types at the Molecular Biology Laboratory of the national Institute of Public Health in Tirana, Albania. Genotyping for hr-HPV 16/18/45 types was performed to positive women. Results: Out of 382 samples, 95 (24.87%) [95% CI, 19.87-29.87] were hr-HPV positive. The prevalence of infection with HPV 16 was 21.05% (95%CI=16.0%-26.1%), whereas the prevalence of infection with HPV 18 was 10.52% (95%CI=5.5%-15.5%). The hr-HPV prevalence in women with abnormal cytology was 27.4%; nonetheless, it was no significantly higher than in women with normal cytology (57.9%). Conclusion: The hr-...

Research paper thumbnail of Genital human papillomavirus infection and genotype prevalence among albanian women: A cross-sectional study

Research paper thumbnail of Genital human papillomavirus infection and genotype prevalence among albanian women: A cross-sectional study

Journal of Medical Virology, 2000

Research paper thumbnail of Oxidative Stress in HPV-Driven Viral Carcinogenesis: Redox Proteomics Analysis of HPV-16 Dysplastic and Neoplastic Tissues

Genital infection by high risk Human Papillomavirus (HR-HPV), although recognized as the main eti... more Genital infection by high risk Human Papillomavirus (HR-HPV), although recognized as the main etio-pathogenetic factor of
cervical cancer, is not per se sufficient to induce tumour development. Oxidative stress (OS) represents an interesting and
under-explored candidate as a promoting factor in HPV-initiated carcinogenesis. To gain insight into the role of OS in
cervical cancer, HPV-16 positive tissues were collected from patients with invasive squamous cervical carcinoma, from
patients with High Grade dysplastic HPV lesions and from patients with no clinical evidence of HPV lesions. After virological
characterization, modulation of proteins involved in the redox status regulation was investigated. ERp57 and GST were
sharply elevated in dysplastic and neoplastic tissues. TrxR2 peaked in dysplastic samples while iNOS was progressively
reduced in dysplastic and neoplastic samples. By redox proteomic approach, five proteins were found to have increased
levels of carbonyls in dysplastic samples respect to controls namely: cytokeratin 6, actin, cornulin, retinal dehydrogenase
and GAPDH. In carcinoma samples the peptidyl-prolyl cis-trans isomerase A, ERp57, serpin B3, Annexin 2 and GAPDH were
found less oxidized than in dysplastic tissues. HPV16 neoplastic progression seems associated with increased oxidant
environment. In dysplastic tissues the oxidative modification of DNA and proteins involved in cell morphogenesis and
terminal differentiation may provide the conditions for the neoplastic progression. Conversely cancer tissues seem to attain
an improved control on oxidative damage as shown by the selective reduction of carbonyl adducts on key detoxifying/prosurvival
proteins