Andrzej Kulig - Academia.edu (original) (raw)
Papers by Andrzej Kulig
Folia Histochemica et Cytobiologica, 2011
Physical Review E, 2015
We investigate properties of evolving linguistic networks defined by the word-adjacency relation.... more We investigate properties of evolving linguistic networks defined by the word-adjacency relation. Such networks belong to the category of networks with accelerated growth but their shortest path length appears to reveal the network size dependence of different functional form than the ones known so far. We thus compare the networks created from literary texts with their artificial substitutes based on different variants of the Dorogovtsev-Mendes model and observe that none of them is able to properly simulate the novel asymptotics of the shortest path length. Then, we identify the local chain-like linear growth induced by grammar and style as a missing element in this model and extend it by incorporating such effects. It is in this way that a satisfactory agreement with the empirical result is obtained.
Sensors
Mineral excavation is a common process throughout the world. The open pits remaining after the cl... more Mineral excavation is a common process throughout the world. The open pits remaining after the closure of a mine require well-considered and meticulous reclamation activities aimed at restoring the environmental properties of a given area. The inspections carried out in Poland indicate numerous irregularities in implementing the reclamation process. The research in this study was conducted in six measurement series and includes both chemical and olfactometry determinations by devices: multisensor portable gas detector and field olfactometer. Statistical analysis of the results obtained show high concentrations in ambient air of both chemical compounds (NH3, VOCs, H2S, CH3SH) and odour, excluding the possibility of occurrence in the pit of only waste types contained in the administrative decision on reclamation. In addition to the unpleasant odour, the listed compounds can have dangerous effects on the health and life of living organisms. This paper presents a suitable method of cont...
Applied Sciences
Municipal waste treatment plants are an important element of the urban area infrastructure, but a... more Municipal waste treatment plants are an important element of the urban area infrastructure, but also, they are a potential source of odour nuisance. Odour impact from municipal waste processing plants raises social concerns regarding the well-being of employees operating the plants and residents of nearby areas. Chemical methods involve the determination of the quantitative composition of compounds comprising odour. These methods are less costly than olfactometry, and their efficiency is not dependent on human response. The relationship between the concentration of a single odorant and its odour threshold (OT) is determined by the odour activity value (OAV) parameter. The research involved the application of a multi-gas detector, MultiRae Pro. Measurements by means of the device were conducted at three municipal waste biogas plants located in Poland. In this paper we describe the results obtained when using a detector during the technological processes, the unitary procedures conduc...
Sustainability
Municipal waste treatment is inherently associated with odour emissions. The compounds characteri... more Municipal waste treatment is inherently associated with odour emissions. The compounds characteristic of the processes used for this purpose, and at the same time causing a negative olfactory sensation, are organic and inorganic sulphur and nitrogen compounds. The tests were carried out at the waste management plant, which in the biological part, uses the methane fermentation process and is also equipped with an installation for the collection, treatment, and energetic use of biogas. The tests include measurements of the four odorant concentrations and emissions, i.e., volatile organic compounds (VOCs), ammonia (NH3), hydrogen sulphide (H2S), and methanethiol (CH3SH). Measurements were made using a MultiRae Pro portable gas detector sensor. The tests were carried out in ten series for twenty measurement points in each series. The results show a significant impact of technological processes on odorant emissions. The types of waste going to the plant are also important in shaping this...
Applied Sciences
Biogas plants processing municipal waste are an important part of a circular economy (energy gene... more Biogas plants processing municipal waste are an important part of a circular economy (energy generation from biogas and organic fertiliser production for the treatment of selectively collected biowaste). However, the technological processes taking place may be associated with odour nuisance. The paper presents the results of pilot research conducted at six municipal waste biogas plants in Poland. It shows the relations between odour intensity and concentration and the occurring meteorological and ambient conditions (air temperature and relative humidity) and technological factors at biogas plants processing municipal waste. The impact of meteorological and ambient conditions was identified by measuring air temperature and relative humidity and observing their changes. The impact of technological factors was identified by measuring odorant concentration (volatile organic compounds and ammonia) and observing their changes between individual measurement series. At most analysed biogas ...
Polish Journal of Pathology Official Journal of the Polish Society of Pathologists, Feb 1, 2004
Immunohistochemical analysis employing a monoclonal antibody nm23-H1 (the antibody against nm-23 ... more Immunohistochemical analysis employing a monoclonal antibody nm23-H1 (the antibody against nm-23 protein) was performed on archival material, consisting of 12 cases of follicular thyroid carcinoma (FTC), 57 cases of follicular thyroid adenoma (FTA) and 17 cases of nodular goiter (NG). Both cytoplasmic and nuclear immunoreactions for nm-23H1 were observed in cells of FTCs, FTAs and NGs. In oxyphilic adenomas cytoplasmic staining was observed. Eleven (91.7%) cases of FTC, 55 (98.2%) cases of FTA and 14 (82.4%) cases of NG were found to be positive for nm23-H1 protein. There were no statistically significant differences in the mean percentage values of immunopositive cells between carcinomas and adenomas. A significant increase in the number of cases with high percentage (more than 50) of positive cells was found in both carcinomas (FTCs) and adenomas (FTAs)--mainly microfollicular ones, in comparison with nodular goiter. It can be concluded that highly positive immunoreaction for the nm23-H1 protein in the cells of carcinomas (FTCs) and microfollicular adenomas indicates for a high proliferation rate of these tumors.
Endokrynologia Polska, 2006
Introduction: In the front of the problems related to the differentiation between benign and mali... more Introduction: In the front of the problems related to the differentiation between benign and malignant thyroid tumors we decided to perform a multicentre study in order to validate diagnoses of malignant thyroid tumors and assess the inter-observer variability. Material and methods: Material included 690 cases of malignant and benign thyroid lesions with primary histopathology established in 1985-1999. These cases were selected to multicentre study. The studies were sent from centres which agreed to participate in the project and than coded in the independent centre-Department of Nuclear Medicine and Endocrine Oncology. 40 pathologists from 25 centres provided their diagnoses which were compared with the reference ones. Results: 10 547 diagnoses were evaluated, both on their accuracy of the distinction between malignant and benign lesions and on their accuracy of cancer histotype definition. The reference diagnosis was made by an agreement between four expert pathologists (D.L., S.S., J.S. and A.K.). The participants diagnosed 21% of cases differently than experts. Concerning the diagnosis of cancer histotype, the difference between participants diagnosis and the reference one was even higher. The best concordance was achieved in the diagnosis of papillary thyroid cancer, however, on the cost of cancer overdiagnosis by some participants. Follicular cancer was diagnosed accurately only in 75.4% of cases. Conclusion: The study documents a high inter-observer variability of thyroid cancer diagnosis and confirms the lesser accuracy of diagnosis of follicular cancer.
Polish Journal of Pathology Official Journal of the Polish Society of Pathologists, 2011
Congenital epulis of newborn is very rare benign intraoral entity of uncertain ethiology. Histolo... more Congenital epulis of newborn is very rare benign intraoral entity of uncertain ethiology. Histologically the lesion is similar to the granular cell tumour of an adult but immunohistochemical stainings prove their different origin. Treatment involves surgical excision, recurrences are rare.
Polish Journal of Pathology Official Journal of the Polish Society of Pathologists, 2004
PI6INK4A (P16) protein expression was analyzed immunohistochemically in archival material derived... more PI6INK4A (P16) protein expression was analyzed immunohistochemically in archival material derived from 12 cases of follicular thyroid carcinoma, 57 cases of follicular adenoma and 17 cases of nodular goiter. Among follicular carcinomas, 11 out of 12 examined cases (91.7%) were positive for P161NK4A protein. Among follicular adenomas the percentage of immunopositivity was 76.5% (45/57) and among nodular goiter cases it was 19.3% (13/17). Overexpression of P16INK4A protein was found in 66.7% (8/12) of follicular carcinomas and in 19.3% (11/57) of follicular adenomas; the values of this parameter were statistically significantly higher in the follicular carcinoma group (p < 0.005). No P16INK4A protein overexpression was noted in nodular goiter cells. High immunohistochemically-detected expression of P16INK4A protein in follicular thyroid carcinoma cells suggests that the altered expression pattern of P16INK4A protein may disturb the regulatory mechanisms of thyreocyte cell cycle and plays a significant role in the formation of benign neoplasms and their malignant counterparts derived from follicular thyroid cell.
Polish Journal of Pathology Official Journal of the Polish Society of Pathologists, Feb 1, 2005
BRCA1 tumor suppressor gene encodes an 1863-amino acid gene product that is implicated in many ce... more BRCA1 tumor suppressor gene encodes an 1863-amino acid gene product that is implicated in many cellular pathways including transcription, cell-cycle checkpoint control, apoptosis and DNA repair. A role of apoptosis and BRCA1 germ-line mutation in breast cancer appearance was investigated in this study by both apoptosis frequency analysis and mutation screening of BRCA1 among breast cancer cases. Blood was obtained from 40 women with node-negative and node-positive ductal breast carcinomas with uniform tumor size. The blood samples from age matched healthy women (n=42) served as control. BRCA1 gene mutations were determined by PCR-RFLP methods. The apoptotic peripheral blood cells were detected by agarose gel electrophoresis. The apoptotic cells were identified in 30% (12/40) of the patients. There were no significant differences in apoptosis frequencies between patients and controls (P > 0.05). Three mutations of BRCA1 gene were identified in apoptosis positive samples from breast cancer women; one Ex20insC and two ExII17delA. Our study implies that apoptosis may be involved not only in sporadic breast carcinoma without BRCA1 mutations, but also in BRCA1-associated breast carcinoma.
Polish Journal of Pathology Official Journal of the Polish Society of Pathologists, Feb 1, 2005
Protein products of cyclin D1 and retinoblastoma (Rb) genes play crucial roles in regulation of G... more Protein products of cyclin D1 and retinoblastoma (Rb) genes play crucial roles in regulation of G1/S transition in the cell cycle. In this study we analyzed, using immunohistochemical methods, the expression of cyclin D1 and Rb proteins in material from medical archives (12 cases of follicular thyroid carcinoma, 57 cases of follicular adenoma and 17 nodular goiter cases). A positive nuclear reaction for cyclin D1 was observed in 83.3% (10/12) of the follicular carcinomas, in 96.5% (55/57) of the follicular adenomas and in 23.5% (4/17) of nodular goiters. Overexpression of cyclin Dl (more than 50% of positively staining cells) was noted in 25% (3/12) of the follicular carcinomas and in 22.8% (13/57) of the follicular adenomas. No overexpression of cyclin D1 was noted among nodular goiters. The number of carcinoma cases with cyclin D1 overexpression did not differ statistically in any significant way from the follicular adenoma group (p = 1.000). A positive nuclear reaction for Rb protein was noted in 100% of the follicular carcinomas (12/12), in 96.5% of the follicular adenomas (55/57) and in 47.1% of the cases (8/17) of nodular goiter. Rb protein overexpression (more than 50% of positively staining cells) was found in 83.3% (10/12) of the follicular carcinomas, in 68.4% (39/57) of the follicular adenomas and in 11.8% (2/17) of the nodular goiters. The number of cases with Rb protein overexpression in the follicular carcinoma group did not differ significantly from that in the follicular adenoma group (p = 0.486). A positive correlation was found in the groups studied between the expressions of Rb protein and cyclin D1. However, the correlation was statistically significant only in the nodular goiter group (Rs = 0.567; p = 0.018). In the follicular carcinoma group, that correlation was borderline (Rp = 0.437; p = 0.072) and, in the follicular adenoma group, it was statistically insignificant (Rs = 0.217; p = 0.105). Our results confirm the existence of mutual regulation mechanisms of Rb and cyclin D1 protein expressions, which are observed in cells from various carcinomas.
Przegląd Gastroenterologiczny
Artykuł oryginalny/Original paper Streszczenie Wstęp: Dotychczas opublikowane prace wskazują na i... more Artykuł oryginalny/Original paper Streszczenie Wstęp: Dotychczas opublikowane prace wskazują na istotną rolę mutacji w genie kodującym trzustkowy inhibitor wydzielania trypsyny (pancreatic secretory trypsin inhibitor-PSTI, określany również jako serine protease inhibitor, Kazal type 1-SPINK1) w patogenezie postaci dziedzicznej i idiopatycznej przewlekłego zapalenia trzustki (PZT). Dane dotyczące roli tej mutacji w patogenezie PZT o etiologii alkoholowej pozostają niewyjaśnione. Poszukuje się ponadto roli mutacji genu SPINK1 w rozwoju sporadycznego raka trzustki (RT). Cel: Ustalenie częstości występowania mutacji SPINK1 u chorych na alkoholowe (PAZT) i idiopatyczne przewlekłe zapalenie trzustki (PIZT), a także na sporadycznego raka trzustki (RT). Poszukiwanie różnic dotyczących przebiegu klinicznego PZT u chorych ze stwierdzonymi mutacjami w porównaniu z chorymi bez mutacji. Materiał i metody: Rozpoznanie PZT i RT ustalano na podstawie obrazu klinicznego, badań metodami ultrasonografii (USG), tomografii komputerowej (TK) i endoskopowej ultrasonografii (endoscopic ultrasonography-EUS). Genomowy DNA wyizolowano z krwi 33 chorych na PAZT, 14 chorych na PIZT, a także 24 chorych na RT. Grupę kontrolną stanowiło 46 pacjentów. Mutację N34S w genie SPINK1 wykrywano z zastosowaniem metody reakcji łańcuchowej polimerazy-polimorfizm długości fragmentów restrykcyjnych (polymerase chain
In natural language using short sentences is considered efficient for communication. However, a t... more In natural language using short sentences is considered efficient for communication. However, a text composed exclusively of such sentences looks technical and reads boring. The text composed of long ones, on the other hand, demands significantly more effort for comprehension. Studying characteristics of the sentence length variability (SLV) in a large corpus of world-famous literary texts shows that an appealing and aesthetic optimum appears somewhere in between and involves selfsimilar, cascade-like alternation of various lengths sentences. A related quantitative observation is that the power spectra S(f) of thus characterised SLV universally develop a convincing `1/f^beta' scaling with the average exponent beta = 1/2, close to what has been identified before in musical compositions or in the brain waves. An overwhelming majority of the studied texts simply obeys such fractal attributes but especially spectacular in this respect are hypertext-like, "stream of consciousnes...
Advances in medical sciences, 2006
Despite innumerous both therapeutic and histopathologic studies that have been performed no morph... more Despite innumerous both therapeutic and histopathologic studies that have been performed no morphologic markers are currently available in order to predict the outcome in patients with laryngeal cancer. According to the recent reports nowadays tumor front grading (TFG) is one of the most reliable methods of estimation of the progress of the changes in the peripheral part of tumor and it seems to be one of the technics, which is able to assess the dynamics of the tumor growth quite precisely. In this sudy it was presented direct relation between morphological features of tumor front and survival. The authors have analysed 120 cases of patients who were operated on the laryngeal squamous cell carcinoma in ENT Department Medical University of Lódź between 1995-2000. Features of the morphologic tumor front grading was performed on H&E-stained sections in the peripheral parts of a tumor. Dependence on tumor grade G, tumor size T, lymph node metastases and survival were analysed. Our stud...
Polish journal of pathology : official journal of the Polish Society of Pathologists, 2007
Hamartomas are the third most common cause of solitary pulmonary nodule and the most common benig... more Hamartomas are the third most common cause of solitary pulmonary nodule and the most common benign tumors of lung. Recent study indicated that hamartoma may be associated with a chronic inflammatory diseases. Histochemical analysis of the expression profile of growth-relevant was shown the upregulation of macrophage migration inhibitory factor (MIF) in hamartomas and surrounding lung parenchyma. We investigated polymorphism G/C at position -173 promoter gene of MIF, pro-inflammatory cytokine in pulmonary hamartoma. This polymorphism of the MIF gene are association with increased production of MIF and have been found to confer increased risk of susceptibility to chronic inflammatory diseases. DNA samples were obtained from hamartoma tissue fixed with formalin, embedded in paraffin, from 52 patients and from blood samples of 123 sex and age matched healthy person served as control. The G/C polymorphism of MIF gene was determined by PCR-based AluI restriction fragment length polimorphi...
Ginekologia polska, 2006
The aims of the study were: 1) evaluation of progress in surgical treatment of gastroschisis betw... more The aims of the study were: 1) evaluation of progress in surgical treatment of gastroschisis between 1990 and 2004 including the impact of prenatal diagnosis; 2) evaluation of mortality and coexisting congenital abnormalities in fetuses and newborns with gastroschisis. Between 1990 and 2004 in Polish Mother's Health Institute gastroschisis was diagnosed in 108 cases. Basing on information from the Department of Pathology, Department of Pediatric Surgery and Department of Fetal Therapy, the patients were divided into 2 groups: 1) miscarried fetuses, still born neonates and non-operated live newborns who died within several hours after delivery (31 cases); 2) live born operated on newborns (77 cases). Until 1999 gastroschisis was diagnosed prenatally only in 30% of patients, while after 1999 the defect was diagnosed in 70% of cases. From the year 2000, due to close cooperation with obstetricians, newborns with gastroschisis have been operated on directly after delivery at the same...
Polski merkuriusz lekarski : organ Polskiego Towarzystwa Lekarskiego, 2006
Microsatellite instability (MSI) is due to defective DNA mismatch repair and is characteristic of... more Microsatellite instability (MSI) is due to defective DNA mismatch repair and is characteristic of HNPCC tumours. The role of MSI in familial predisposition to colorectal cancer was investigated in this study by microsatellite analysis among familial cases. PCR-based microsatellite analysis was performed in blood obtained from 30 members from HNPCC families. Blood samples age matched healthy individuals (n = 28) served as control. MSI was studied at five loci containing single- or dinucleotide repeat sequences and mapping to different chromosomal locations: BAT-25 (at locus 4q12), BAT-26 (2p16), D2S123 (2p16-p21), D5S346 (5q21-q22) and D17S250 (17q11.2-q12). MSI frequency was higher in member of HNPCC families (40%) than in control (10.7%) cases. Out of the 30 samples tested, 12 were found to be MSI positive, 9 MSI high and 3 MSI low. The present study suggest that microsatellite instability seem to be a risk factor of colorectal cancer in subjects belonged to HNPCC families with hig...
Ginekologia polska, 2005
Early amnion rupture (EAR, EARS, EAROD) may be the cause of amniotic bands and sheets development... more Early amnion rupture (EAR, EARS, EAROD) may be the cause of amniotic bands and sheets development, which may lead to different fetal defects--from minor finger amputation, chiloschisis or palatoschisis to major amputation, acranius, deep paroschisis or broad body covering cleavage. The aim of this report was to analyse cases of fetal malformation, which might have been caused by EARS. A retrospective analysis of 3173 autopsies performed within a span of 14 years (1989-2003). Among 744 cases of malformations 14 cases of fetuses and newborns presented developmental anomalies, which probably were the consequences of EARS. The EARS is probably more frequent than it is generally presumed, but it is very often impossible to find remnants of bands and sheets. Nevertheless, the presence of at least two typical anomalies excludes a different cause than early amnion rupture and amniotic bands.
Polski merkuriusz lekarski : organ Polskiego Towarzystwa Lekarskiego, 2005
The authors described 6 cases of serious complication of the multiple pregnancies with twin-to-tw... more The authors described 6 cases of serious complication of the multiple pregnancies with twin-to-twin transfusion syndrome (TTTS) sub form twin reverse arterial perfusion (TRAP) with development of acardiac fetus. It was established that this anomaly is an extremely rare complication of multiple pregnancies and very uncommon congenital malformation disclosed in fetuses and newborns. Acardiac "recipient" fetuses may have different morphological forms and often their birth weight is higher than that of the "donor" fetuses. The latter represent morphologic features of circulatory overload due to the necessity of supplying blood to the amorphous acardiac fetus. They are usually born preterm with circulatory insufficiency, intrauterine hypoxia and with developmental anomalies. Survival of one of the "donor" fetus implies the necessity of early diagnosis to detect pregnancy pathology, possible intrauterine intervention and monitoring of the healthy twin.
Folia Histochemica et Cytobiologica, 2011
Physical Review E, 2015
We investigate properties of evolving linguistic networks defined by the word-adjacency relation.... more We investigate properties of evolving linguistic networks defined by the word-adjacency relation. Such networks belong to the category of networks with accelerated growth but their shortest path length appears to reveal the network size dependence of different functional form than the ones known so far. We thus compare the networks created from literary texts with their artificial substitutes based on different variants of the Dorogovtsev-Mendes model and observe that none of them is able to properly simulate the novel asymptotics of the shortest path length. Then, we identify the local chain-like linear growth induced by grammar and style as a missing element in this model and extend it by incorporating such effects. It is in this way that a satisfactory agreement with the empirical result is obtained.
Sensors
Mineral excavation is a common process throughout the world. The open pits remaining after the cl... more Mineral excavation is a common process throughout the world. The open pits remaining after the closure of a mine require well-considered and meticulous reclamation activities aimed at restoring the environmental properties of a given area. The inspections carried out in Poland indicate numerous irregularities in implementing the reclamation process. The research in this study was conducted in six measurement series and includes both chemical and olfactometry determinations by devices: multisensor portable gas detector and field olfactometer. Statistical analysis of the results obtained show high concentrations in ambient air of both chemical compounds (NH3, VOCs, H2S, CH3SH) and odour, excluding the possibility of occurrence in the pit of only waste types contained in the administrative decision on reclamation. In addition to the unpleasant odour, the listed compounds can have dangerous effects on the health and life of living organisms. This paper presents a suitable method of cont...
Applied Sciences
Municipal waste treatment plants are an important element of the urban area infrastructure, but a... more Municipal waste treatment plants are an important element of the urban area infrastructure, but also, they are a potential source of odour nuisance. Odour impact from municipal waste processing plants raises social concerns regarding the well-being of employees operating the plants and residents of nearby areas. Chemical methods involve the determination of the quantitative composition of compounds comprising odour. These methods are less costly than olfactometry, and their efficiency is not dependent on human response. The relationship between the concentration of a single odorant and its odour threshold (OT) is determined by the odour activity value (OAV) parameter. The research involved the application of a multi-gas detector, MultiRae Pro. Measurements by means of the device were conducted at three municipal waste biogas plants located in Poland. In this paper we describe the results obtained when using a detector during the technological processes, the unitary procedures conduc...
Sustainability
Municipal waste treatment is inherently associated with odour emissions. The compounds characteri... more Municipal waste treatment is inherently associated with odour emissions. The compounds characteristic of the processes used for this purpose, and at the same time causing a negative olfactory sensation, are organic and inorganic sulphur and nitrogen compounds. The tests were carried out at the waste management plant, which in the biological part, uses the methane fermentation process and is also equipped with an installation for the collection, treatment, and energetic use of biogas. The tests include measurements of the four odorant concentrations and emissions, i.e., volatile organic compounds (VOCs), ammonia (NH3), hydrogen sulphide (H2S), and methanethiol (CH3SH). Measurements were made using a MultiRae Pro portable gas detector sensor. The tests were carried out in ten series for twenty measurement points in each series. The results show a significant impact of technological processes on odorant emissions. The types of waste going to the plant are also important in shaping this...
Applied Sciences
Biogas plants processing municipal waste are an important part of a circular economy (energy gene... more Biogas plants processing municipal waste are an important part of a circular economy (energy generation from biogas and organic fertiliser production for the treatment of selectively collected biowaste). However, the technological processes taking place may be associated with odour nuisance. The paper presents the results of pilot research conducted at six municipal waste biogas plants in Poland. It shows the relations between odour intensity and concentration and the occurring meteorological and ambient conditions (air temperature and relative humidity) and technological factors at biogas plants processing municipal waste. The impact of meteorological and ambient conditions was identified by measuring air temperature and relative humidity and observing their changes. The impact of technological factors was identified by measuring odorant concentration (volatile organic compounds and ammonia) and observing their changes between individual measurement series. At most analysed biogas ...
Polish Journal of Pathology Official Journal of the Polish Society of Pathologists, Feb 1, 2004
Immunohistochemical analysis employing a monoclonal antibody nm23-H1 (the antibody against nm-23 ... more Immunohistochemical analysis employing a monoclonal antibody nm23-H1 (the antibody against nm-23 protein) was performed on archival material, consisting of 12 cases of follicular thyroid carcinoma (FTC), 57 cases of follicular thyroid adenoma (FTA) and 17 cases of nodular goiter (NG). Both cytoplasmic and nuclear immunoreactions for nm-23H1 were observed in cells of FTCs, FTAs and NGs. In oxyphilic adenomas cytoplasmic staining was observed. Eleven (91.7%) cases of FTC, 55 (98.2%) cases of FTA and 14 (82.4%) cases of NG were found to be positive for nm23-H1 protein. There were no statistically significant differences in the mean percentage values of immunopositive cells between carcinomas and adenomas. A significant increase in the number of cases with high percentage (more than 50) of positive cells was found in both carcinomas (FTCs) and adenomas (FTAs)--mainly microfollicular ones, in comparison with nodular goiter. It can be concluded that highly positive immunoreaction for the nm23-H1 protein in the cells of carcinomas (FTCs) and microfollicular adenomas indicates for a high proliferation rate of these tumors.
Endokrynologia Polska, 2006
Introduction: In the front of the problems related to the differentiation between benign and mali... more Introduction: In the front of the problems related to the differentiation between benign and malignant thyroid tumors we decided to perform a multicentre study in order to validate diagnoses of malignant thyroid tumors and assess the inter-observer variability. Material and methods: Material included 690 cases of malignant and benign thyroid lesions with primary histopathology established in 1985-1999. These cases were selected to multicentre study. The studies were sent from centres which agreed to participate in the project and than coded in the independent centre-Department of Nuclear Medicine and Endocrine Oncology. 40 pathologists from 25 centres provided their diagnoses which were compared with the reference ones. Results: 10 547 diagnoses were evaluated, both on their accuracy of the distinction between malignant and benign lesions and on their accuracy of cancer histotype definition. The reference diagnosis was made by an agreement between four expert pathologists (D.L., S.S., J.S. and A.K.). The participants diagnosed 21% of cases differently than experts. Concerning the diagnosis of cancer histotype, the difference between participants diagnosis and the reference one was even higher. The best concordance was achieved in the diagnosis of papillary thyroid cancer, however, on the cost of cancer overdiagnosis by some participants. Follicular cancer was diagnosed accurately only in 75.4% of cases. Conclusion: The study documents a high inter-observer variability of thyroid cancer diagnosis and confirms the lesser accuracy of diagnosis of follicular cancer.
Polish Journal of Pathology Official Journal of the Polish Society of Pathologists, 2011
Congenital epulis of newborn is very rare benign intraoral entity of uncertain ethiology. Histolo... more Congenital epulis of newborn is very rare benign intraoral entity of uncertain ethiology. Histologically the lesion is similar to the granular cell tumour of an adult but immunohistochemical stainings prove their different origin. Treatment involves surgical excision, recurrences are rare.
Polish Journal of Pathology Official Journal of the Polish Society of Pathologists, 2004
PI6INK4A (P16) protein expression was analyzed immunohistochemically in archival material derived... more PI6INK4A (P16) protein expression was analyzed immunohistochemically in archival material derived from 12 cases of follicular thyroid carcinoma, 57 cases of follicular adenoma and 17 cases of nodular goiter. Among follicular carcinomas, 11 out of 12 examined cases (91.7%) were positive for P161NK4A protein. Among follicular adenomas the percentage of immunopositivity was 76.5% (45/57) and among nodular goiter cases it was 19.3% (13/17). Overexpression of P16INK4A protein was found in 66.7% (8/12) of follicular carcinomas and in 19.3% (11/57) of follicular adenomas; the values of this parameter were statistically significantly higher in the follicular carcinoma group (p < 0.005). No P16INK4A protein overexpression was noted in nodular goiter cells. High immunohistochemically-detected expression of P16INK4A protein in follicular thyroid carcinoma cells suggests that the altered expression pattern of P16INK4A protein may disturb the regulatory mechanisms of thyreocyte cell cycle and plays a significant role in the formation of benign neoplasms and their malignant counterparts derived from follicular thyroid cell.
Polish Journal of Pathology Official Journal of the Polish Society of Pathologists, Feb 1, 2005
BRCA1 tumor suppressor gene encodes an 1863-amino acid gene product that is implicated in many ce... more BRCA1 tumor suppressor gene encodes an 1863-amino acid gene product that is implicated in many cellular pathways including transcription, cell-cycle checkpoint control, apoptosis and DNA repair. A role of apoptosis and BRCA1 germ-line mutation in breast cancer appearance was investigated in this study by both apoptosis frequency analysis and mutation screening of BRCA1 among breast cancer cases. Blood was obtained from 40 women with node-negative and node-positive ductal breast carcinomas with uniform tumor size. The blood samples from age matched healthy women (n=42) served as control. BRCA1 gene mutations were determined by PCR-RFLP methods. The apoptotic peripheral blood cells were detected by agarose gel electrophoresis. The apoptotic cells were identified in 30% (12/40) of the patients. There were no significant differences in apoptosis frequencies between patients and controls (P > 0.05). Three mutations of BRCA1 gene were identified in apoptosis positive samples from breast cancer women; one Ex20insC and two ExII17delA. Our study implies that apoptosis may be involved not only in sporadic breast carcinoma without BRCA1 mutations, but also in BRCA1-associated breast carcinoma.
Polish Journal of Pathology Official Journal of the Polish Society of Pathologists, Feb 1, 2005
Protein products of cyclin D1 and retinoblastoma (Rb) genes play crucial roles in regulation of G... more Protein products of cyclin D1 and retinoblastoma (Rb) genes play crucial roles in regulation of G1/S transition in the cell cycle. In this study we analyzed, using immunohistochemical methods, the expression of cyclin D1 and Rb proteins in material from medical archives (12 cases of follicular thyroid carcinoma, 57 cases of follicular adenoma and 17 nodular goiter cases). A positive nuclear reaction for cyclin D1 was observed in 83.3% (10/12) of the follicular carcinomas, in 96.5% (55/57) of the follicular adenomas and in 23.5% (4/17) of nodular goiters. Overexpression of cyclin Dl (more than 50% of positively staining cells) was noted in 25% (3/12) of the follicular carcinomas and in 22.8% (13/57) of the follicular adenomas. No overexpression of cyclin D1 was noted among nodular goiters. The number of carcinoma cases with cyclin D1 overexpression did not differ statistically in any significant way from the follicular adenoma group (p = 1.000). A positive nuclear reaction for Rb protein was noted in 100% of the follicular carcinomas (12/12), in 96.5% of the follicular adenomas (55/57) and in 47.1% of the cases (8/17) of nodular goiter. Rb protein overexpression (more than 50% of positively staining cells) was found in 83.3% (10/12) of the follicular carcinomas, in 68.4% (39/57) of the follicular adenomas and in 11.8% (2/17) of the nodular goiters. The number of cases with Rb protein overexpression in the follicular carcinoma group did not differ significantly from that in the follicular adenoma group (p = 0.486). A positive correlation was found in the groups studied between the expressions of Rb protein and cyclin D1. However, the correlation was statistically significant only in the nodular goiter group (Rs = 0.567; p = 0.018). In the follicular carcinoma group, that correlation was borderline (Rp = 0.437; p = 0.072) and, in the follicular adenoma group, it was statistically insignificant (Rs = 0.217; p = 0.105). Our results confirm the existence of mutual regulation mechanisms of Rb and cyclin D1 protein expressions, which are observed in cells from various carcinomas.
Przegląd Gastroenterologiczny
Artykuł oryginalny/Original paper Streszczenie Wstęp: Dotychczas opublikowane prace wskazują na i... more Artykuł oryginalny/Original paper Streszczenie Wstęp: Dotychczas opublikowane prace wskazują na istotną rolę mutacji w genie kodującym trzustkowy inhibitor wydzielania trypsyny (pancreatic secretory trypsin inhibitor-PSTI, określany również jako serine protease inhibitor, Kazal type 1-SPINK1) w patogenezie postaci dziedzicznej i idiopatycznej przewlekłego zapalenia trzustki (PZT). Dane dotyczące roli tej mutacji w patogenezie PZT o etiologii alkoholowej pozostają niewyjaśnione. Poszukuje się ponadto roli mutacji genu SPINK1 w rozwoju sporadycznego raka trzustki (RT). Cel: Ustalenie częstości występowania mutacji SPINK1 u chorych na alkoholowe (PAZT) i idiopatyczne przewlekłe zapalenie trzustki (PIZT), a także na sporadycznego raka trzustki (RT). Poszukiwanie różnic dotyczących przebiegu klinicznego PZT u chorych ze stwierdzonymi mutacjami w porównaniu z chorymi bez mutacji. Materiał i metody: Rozpoznanie PZT i RT ustalano na podstawie obrazu klinicznego, badań metodami ultrasonografii (USG), tomografii komputerowej (TK) i endoskopowej ultrasonografii (endoscopic ultrasonography-EUS). Genomowy DNA wyizolowano z krwi 33 chorych na PAZT, 14 chorych na PIZT, a także 24 chorych na RT. Grupę kontrolną stanowiło 46 pacjentów. Mutację N34S w genie SPINK1 wykrywano z zastosowaniem metody reakcji łańcuchowej polimerazy-polimorfizm długości fragmentów restrykcyjnych (polymerase chain
In natural language using short sentences is considered efficient for communication. However, a t... more In natural language using short sentences is considered efficient for communication. However, a text composed exclusively of such sentences looks technical and reads boring. The text composed of long ones, on the other hand, demands significantly more effort for comprehension. Studying characteristics of the sentence length variability (SLV) in a large corpus of world-famous literary texts shows that an appealing and aesthetic optimum appears somewhere in between and involves selfsimilar, cascade-like alternation of various lengths sentences. A related quantitative observation is that the power spectra S(f) of thus characterised SLV universally develop a convincing `1/f^beta' scaling with the average exponent beta = 1/2, close to what has been identified before in musical compositions or in the brain waves. An overwhelming majority of the studied texts simply obeys such fractal attributes but especially spectacular in this respect are hypertext-like, "stream of consciousnes...
Advances in medical sciences, 2006
Despite innumerous both therapeutic and histopathologic studies that have been performed no morph... more Despite innumerous both therapeutic and histopathologic studies that have been performed no morphologic markers are currently available in order to predict the outcome in patients with laryngeal cancer. According to the recent reports nowadays tumor front grading (TFG) is one of the most reliable methods of estimation of the progress of the changes in the peripheral part of tumor and it seems to be one of the technics, which is able to assess the dynamics of the tumor growth quite precisely. In this sudy it was presented direct relation between morphological features of tumor front and survival. The authors have analysed 120 cases of patients who were operated on the laryngeal squamous cell carcinoma in ENT Department Medical University of Lódź between 1995-2000. Features of the morphologic tumor front grading was performed on H&E-stained sections in the peripheral parts of a tumor. Dependence on tumor grade G, tumor size T, lymph node metastases and survival were analysed. Our stud...
Polish journal of pathology : official journal of the Polish Society of Pathologists, 2007
Hamartomas are the third most common cause of solitary pulmonary nodule and the most common benig... more Hamartomas are the third most common cause of solitary pulmonary nodule and the most common benign tumors of lung. Recent study indicated that hamartoma may be associated with a chronic inflammatory diseases. Histochemical analysis of the expression profile of growth-relevant was shown the upregulation of macrophage migration inhibitory factor (MIF) in hamartomas and surrounding lung parenchyma. We investigated polymorphism G/C at position -173 promoter gene of MIF, pro-inflammatory cytokine in pulmonary hamartoma. This polymorphism of the MIF gene are association with increased production of MIF and have been found to confer increased risk of susceptibility to chronic inflammatory diseases. DNA samples were obtained from hamartoma tissue fixed with formalin, embedded in paraffin, from 52 patients and from blood samples of 123 sex and age matched healthy person served as control. The G/C polymorphism of MIF gene was determined by PCR-based AluI restriction fragment length polimorphi...
Ginekologia polska, 2006
The aims of the study were: 1) evaluation of progress in surgical treatment of gastroschisis betw... more The aims of the study were: 1) evaluation of progress in surgical treatment of gastroschisis between 1990 and 2004 including the impact of prenatal diagnosis; 2) evaluation of mortality and coexisting congenital abnormalities in fetuses and newborns with gastroschisis. Between 1990 and 2004 in Polish Mother's Health Institute gastroschisis was diagnosed in 108 cases. Basing on information from the Department of Pathology, Department of Pediatric Surgery and Department of Fetal Therapy, the patients were divided into 2 groups: 1) miscarried fetuses, still born neonates and non-operated live newborns who died within several hours after delivery (31 cases); 2) live born operated on newborns (77 cases). Until 1999 gastroschisis was diagnosed prenatally only in 30% of patients, while after 1999 the defect was diagnosed in 70% of cases. From the year 2000, due to close cooperation with obstetricians, newborns with gastroschisis have been operated on directly after delivery at the same...
Polski merkuriusz lekarski : organ Polskiego Towarzystwa Lekarskiego, 2006
Microsatellite instability (MSI) is due to defective DNA mismatch repair and is characteristic of... more Microsatellite instability (MSI) is due to defective DNA mismatch repair and is characteristic of HNPCC tumours. The role of MSI in familial predisposition to colorectal cancer was investigated in this study by microsatellite analysis among familial cases. PCR-based microsatellite analysis was performed in blood obtained from 30 members from HNPCC families. Blood samples age matched healthy individuals (n = 28) served as control. MSI was studied at five loci containing single- or dinucleotide repeat sequences and mapping to different chromosomal locations: BAT-25 (at locus 4q12), BAT-26 (2p16), D2S123 (2p16-p21), D5S346 (5q21-q22) and D17S250 (17q11.2-q12). MSI frequency was higher in member of HNPCC families (40%) than in control (10.7%) cases. Out of the 30 samples tested, 12 were found to be MSI positive, 9 MSI high and 3 MSI low. The present study suggest that microsatellite instability seem to be a risk factor of colorectal cancer in subjects belonged to HNPCC families with hig...
Ginekologia polska, 2005
Early amnion rupture (EAR, EARS, EAROD) may be the cause of amniotic bands and sheets development... more Early amnion rupture (EAR, EARS, EAROD) may be the cause of amniotic bands and sheets development, which may lead to different fetal defects--from minor finger amputation, chiloschisis or palatoschisis to major amputation, acranius, deep paroschisis or broad body covering cleavage. The aim of this report was to analyse cases of fetal malformation, which might have been caused by EARS. A retrospective analysis of 3173 autopsies performed within a span of 14 years (1989-2003). Among 744 cases of malformations 14 cases of fetuses and newborns presented developmental anomalies, which probably were the consequences of EARS. The EARS is probably more frequent than it is generally presumed, but it is very often impossible to find remnants of bands and sheets. Nevertheless, the presence of at least two typical anomalies excludes a different cause than early amnion rupture and amniotic bands.
Polski merkuriusz lekarski : organ Polskiego Towarzystwa Lekarskiego, 2005
The authors described 6 cases of serious complication of the multiple pregnancies with twin-to-tw... more The authors described 6 cases of serious complication of the multiple pregnancies with twin-to-twin transfusion syndrome (TTTS) sub form twin reverse arterial perfusion (TRAP) with development of acardiac fetus. It was established that this anomaly is an extremely rare complication of multiple pregnancies and very uncommon congenital malformation disclosed in fetuses and newborns. Acardiac "recipient" fetuses may have different morphological forms and often their birth weight is higher than that of the "donor" fetuses. The latter represent morphologic features of circulatory overload due to the necessity of supplying blood to the amorphous acardiac fetus. They are usually born preterm with circulatory insufficiency, intrauterine hypoxia and with developmental anomalies. Survival of one of the "donor" fetus implies the necessity of early diagnosis to detect pregnancy pathology, possible intrauterine intervention and monitoring of the healthy twin.