Kumud Singh - Academia.edu (original) (raw)

Papers by Kumud Singh

In current social networking world, organizations are demanding high-speed security for client da... more In current social networking world, organizations are demanding high-speed security for client data such as images, audio video files even huge databases. Also no flexibility to connect and share remote devices and servers reduces the performance of service given by organization. To overcome this issues Storage Area Network (SAN) was introduced which is a dedicated storage network that carries I/O traffic only between servers and storage devices. The paper describes requirement of SAN in real world by implementing a SAN using Openfiler, Based on seven cases SAN’s performance analysis done with help of Iometer tool and SAN’s importance in clustered Database.

BioTechniques, 2000

Fluorescence polarization has been used recently to monitor diverse macromolecular interactions. ... more Fluorescence polarization has been used recently to monitor diverse macromolecular interactions. In this report, the application of fluorescence polarization has been extended to monitor ribozyme reactions in real time. With fluorescently labeled substrate RNAs, group I ribozyme ligation and hammerhead ribozyme cleavage reactions were studied by fluorescence polarization in substrate excess (multiple turnover) conditions. These results also show that fluorescently labeled RNAs remain active substrates for ribozymes. Furthermore, a direct comparison of fluorescence polarization with fluorescence resonance energy transfer showed that both techniques were comparable for monitoring ribozyme reactions.

Journal of the Indian Society of Soil Science, 1993

INTERNATIONAL JOURNAL OF FORESTRY AND CROP IMPROVEMENT, 2015

Future HIV Therapy, 2008

Several host factors, that is, factors that at least in part could protect individuals from becom... more Several host factors, that is, factors that at least in part could protect individuals from becoming HIV infected, have been described. Individuals who do not become infected despite repeated exposure are exposed, uninfected individuals. In this review, we briefly describe some host factors that might contribute to protection from HIV infection in these exposed uninfected individuals. Alteration in chemokine levels and chemokine receptors were the first of these factors described that are associated with some exposed uninfected individuals. More recently, mutations in cytokine factors, notably interferon regulatory factor 1, have been described in a Kenyan commercial sex workers cohort who have remained uninfected despite multiple exposures to HIV-infected clients. Receptors on dendritic cells offer the opportunity to protect individuals where transmission of the virus would occur by the mucosal route. Similarly, several host restriction factors have anti-HIV activity; these include...

Indian journal of physiology and pharmacology, 2002

Left ventricular function (LVF) was evaluated noninvasively by recording systolic time intervals ... more Left ventricular function (LVF) was evaluated noninvasively by recording systolic time intervals (STIs) in 25 patients suffering from varicose veins (VV) of lower limbs and compared with 25 age--sex matched controls. STI was measured from simultaneous recordings of electrocardiogram (ECG), carotid arterial pulse (CAP) and phonocardiogram (PCG). The left ventricular ejection time (LVET) was significantly shortened with marked increase in pre-ejection period (PEP) and increase in PEP/LVET ratio (P < 0.001) without any variation in electromechanical systoles (QS2) in VV patients compared to controls. These changes in STI suggest contractility of heart is adversely affected in VV patients.

2010 2nd International Conference on Reliability, Safety and Hazard - Risk-Based Technologies and Physics-of-Failure Methods (ICRESH), 2010

Noise limits set by regulatory agencies make solutions to common mode EMI a necessary considerati... more Noise limits set by regulatory agencies make solutions to common mode EMI a necessary consideration in the manufacture and use of electronic equipment. Common mode filters are generally relied upon to suppress line conducted common mode interference. These filters successfully and reliably reduce common mode noise. However, successful design of common mode filters requires foresight into the non ideal character

2014 International Symposium on Discharges and Electrical Insulation in Vacuum (ISDEIV), 2014

Alvarez type Drift Tube Linac (DTL) is used for acceleration of proton beam in low energy section... more Alvarez type Drift Tube Linac (DTL) is used for acceleration of proton beam in low energy section of beta ranging from 0.04 to 0.40. DTL is cylindrical RF cavity resonating in TM010 mode at 352.21 MHz frequency. It consists of array of drift tubes arranged ensuring that DTL centre and Drift Tube centre are concentric. The Drift Tubes also houses Permanent Magnet Quadrupole for transverse focusing of proton beam. A twelve cell prototype of DTL section is designed, developed and fabricated at Bhabha Atomic Research Centre, Trombay. Complete DTL accelerator consists of eight such DTL sections. High frequency microwave simulations are carried out in SOPRANO, vector fields and COMSOL simulation software. This prototype DTL is 1640.56 mm long cavity with 520mm ID, 600mm OD and consists of eleven Drift Tubes, two RF end flanges, three slug tuners, six post couplers, three RF field monitors, one RF waveguide coupler, two DN100 vacuum flanges and DTL tank platform with alignment features. Girder based Drift tube mounting arrangement utilizing uncompressing energy of disc springs for optimum combo RF-vacuum seal compression is worked out and implemented. This paper discusses design of this RF vacuum cavity operating at high accelerating field gradient in ultra-high vacuum. Detailed vacuum design and results of RF and vacuum qualifications are discussed. Results on mechanical accuracy achieved on scaled pre-prototype are also presented. Paper summarizes the engineering developments carried out for this RF cavity and brings out the future activities proposed in indigenous development of high gradient RF cavities for ion accelerators.

Small

The main objective of this study was to analysis the yield and net return in pigeon pea among the... more The main objective of this study was to analysis the yield and net return in pigeon pea among the randomly selected small, medium and large size of the farmers in selected village Sabua during 2008-2009 in Ghazipur district of Uttar Pradesh. It was found that ...

Pharmacognosy Journal, 2010

Antisense and Nucleic Acid Drug Development, 1996

Hammerhead ribozymes are attractive tools in antisense gene inactivation because of their catalyt... more Hammerhead ribozymes are attractive tools in antisense gene inactivation because of their catalytic cleavage of target molecules. High sequence discrimination should be possible, since the cleavage efficiencies were already significantly reduced, if single base changes in substrate RNA introduce mismatches next to the cleavage site. This was observed at the first innermost base pair in helix I and the two innermost base pairs in helix III. In addition to its position, the nature of the mismatch pair was important.

The Journal of Infectious Diseases, 2003

The role that host genetics plays in the modification of the rate of human immunodeficiency virus... more The role that host genetics plays in the modification of the rate of human immunodeficiency virus 1 (HIV-1)-related disease progression was evaluated in a seroprevalent cohort of 1049 children with symptomatic HIV-1 infection who participated in 2 clinical trials in the United States. Variants including CCR2-V64I, CCR5wt/D32, CCR5-59029-G/A, CCR5-59353-T/C, CCR5-59356-C/T, and SDF1-3-G/A were identified by polymerase chain-reaction genotyping. Children with the CCR5-wt/D32 genotype experienced significantly delayed disease progression, including less neurocognitive impairment. In the CCR5-wt/wt group, the most rapid disease progression was in those with the CCR5-59029-A/A genotype, which was present in 23% of the children. Although the SDF1-3-A/A variant was associated with more-rapid disease progression, it occurred in !2% of the children studied. Modest or little impact was associated with the CCR5-59353, CCR5-59356, or CCR2 genotypes. Thus, in children with the CCR5-wt/wt genotype, variants at CCR5-59029 have the broadest impact on disease progression. These data suggest that, in children, host genetics plays an important role in HIV-1related disease progression and neurological impairment. Identification of virologic and immunologic factors that have an impact on transmission of human immunodeficiency virus type 1 (HIV-1) and disease progression has provided valuable tools for monitoring antiretroviral therapy and for assessing the risk of mother-toinfant transmission. With increased knowledge of HIV

The Journal of Infectious Diseases, 2005

Background. The impact of CX 3 CR1 polymorphisms on human immunodeficiency virus type 1 (HIV-1) p... more Background. The impact of CX 3 CR1 polymorphisms on human immunodeficiency virus type 1 (HIV-1) pathogenesis is controversial, with conflicting reports of their role in disease progression in HIV-1-infected adults. Methods. A cohort of 1055 HIV-1-infected children were genotyped for 2 CX 3 CR1 polymorphisms, V/I249 and T/M280, and their impact on HIV-1-related disease progression, including central nervous system (CNS) impairment, was evaluated. Results. Children with the CX 3 CR1 I/I249 genotype experienced more-rapid disease progression (I/I249 vs. V/ V249: relative hazard [RH], 2.19 [95% confidence interval {CI}, 1.30-3.68], ; I/I249 vs. V/I249: RH, 1.77 P p .003 [95% CI, 1.00-3.14],) and a trend toward more CNS impairment (I/I249 vs. V/V249: RH, 2.19 [95% CI, P p .05 1.00-4.78], ; I/I249 vs. V/I249: RH, 2.02 [95% CI, 0.85-4.83],). Children with the V249-T280 P p .049 P p .11 haplotype experienced significantly less disease progression (RH, 0.42 [95% CI, 0.24-0.73];) and CNS P p .002 impairment (RH, 0.39 [95% CI, 0.39-0.22];). Of note, these effects remained significant after CD4 + lym-P p .001 phocyte count and plasma HIV-1 RNA load at baseline were adjusted for and in a longitudinal, multivariate analysis. Conclusions. CX 3 CR1 genotypes and haplotypes impact HIV-1 disease progression independently of CD4 + lymphocyte count and plasma HIV-1 RNA load, suggesting that the fundamental role of CX 3 CR1 in the alteration of disease progression might be the recruitment of immunomodulatory cells responsible for the control of HIV-1. Human chemokines and chemokine receptors are involved in HIV infection and disease progression [1]. CX 3 CR1 belongs to a family of G-protein-coupled receptors and is a leukocyte chemotactic and adhesion receptor for fractalkine [2]. CX 3 CR1 is a recently identified HIV-1 coreceptor [3] that is expressed in monocytes, T lymphocytes, NK cells, astrocytes, neurons, brain microglia, and many tissues, with particularly high levels present in the brain [3, 4].

Journal of Biosciences, 1998

Abstract Self-cleaving infectious RNAs found in many plant viruses and viroids can also cleave in... more Abstract Self-cleaving infectious RNAs found in many plant viruses and viroids can also cleave intrans and form hammerhead type secondary structure. It has been observed that the cleavage site must contain the triplet GUC. Also, in other cases, the sequence XUY ...

Journal of Allergy and Clinical Immunology, 2008