L. Girosi - Academia.edu (original) (raw)

Papers by L. Girosi

Research paper thumbnail of Il sistema GABAergico in Eunicella cavolini

Research paper thumbnail of Immunohistochemical FMRFamide-like distribution in the cyprid of the barnacle Balanus amphitrite

Research paper thumbnail of PACAP in developing sensory and peripheral organs of the zebrafish, Danio rerio

European journal of histochemistry : EJH

The anatomical distribution of PACAP-like immunoreactivity was investigated in sensory and periph... more The anatomical distribution of PACAP-like immunoreactivity was investigated in sensory and peripheral organs of the zebrafish, Danio rerio, during the pharyngula, hatching and larval periods, by using indirect immunofluorescence methods. First PACAP-like immunoreactive (ir) elements appeared during the pharyngula period, at 24 hours post fertilization (hpf), within the most superficial layer of the retina and the dorsal aorta. At 48 hpf, additional ir cells were found in the olfactory placode and esophagus. At 72 hpf (hatching period), PACAP-like immunoreactivity was first detected in the ganglion cell layer of the retina, the otic sensory epithelium, pharyngeal arches, swim bladder and pancreatic progenitor cells. During day 5 of larval development, new groups of ir cells appeared in the liver, whereas no ir elements were observed in the olfactory placode. Subsequently, at day 13 of larval development, additional ir elements were found for the first time in some gut epithelial cell...

Research paper thumbnail of First record of albinism in the deep-water shark Dalatias licha

Marine Biodiversity Records, 2008

ABSTRACT The first case of albinism in the kitefin shark, Dalatias licha, is reported here throug... more ABSTRACT The first case of albinism in the kitefin shark, Dalatias licha, is reported here through the study of one specimen caught by professional bottom trawl in the Gulf of Genoa, Ligurian Sea. The albino surface was quantified and morphometric and meristic characteristics were compared with those of normal specimens of the same sex and equivalent length, caught within the same area. The shark body presented a partial reduction in pigmentation and no morphometric differences between the albino specimen and the normal individuals were observed.

Research paper thumbnail of Apoptosis, cell proliferation and serotonin immunoreactivity in gut of Liza aurata from natural heavy metal polluted environments: preliminary observations

European Journal of Histochemistry, 2005

Research paper thumbnail of Presence and distribution of serotonin immunoreactivity in the cyprids of the barnacle Balanus amphitrite

European Journal of Histochemistry, 2005

The presence and distribution of FMRFamide-like peptides (FLPs) in the cyprid larvae of the barna... more The presence and distribution of FMRFamide-like peptides (FLPs) in the cyprid larvae of the barnacle Balanus amphitrite were investigated using immunohistochemical methods. Barnacles are considered to be one of the most important constituents of animal fouling communities, and the cyprid stage is specialized for settlement and metamorphosis in to the sessile adult condition. FLPs immunoreactive (IR) neuronal cell bodies were detected in both the central and the peripheral nervous system. One bilateral group of neurons somata was immunodetected in the brain, and IR nerve fibers were observed in the neuropil area and optic lobes. Intense immunostaining was also observed in the frontal filament complex: frontal filament tracts leaving the optic lobes and projecting towards the compound eyes, swollen nerve endings in the frontal filament vesicles, and thin nerve endings in the external frontal filament. Thin IR nerve fibers were also present in the cement glands. Two pairs of neuronal cell bodies were immunodetected in the posterior ganglion; some of their axons appear to project to the cirri. FLPs IR neuronal cell bodies were also localized in the wall of the dilated midgut and in the narrow hindgut; their processes surround the gut wall and allow gut neurons to synapse with one another. Our data demonstrated the presence of FLPs IR substances in the barnacle cyprid. We hypothesize that these peptides act as integrators in the central nervous system, perform neuromuscular functions for thoracic limbs, trigger intestinal movements and, at the level of the frontal filament, play a neurosecretory role. Microsc. Res. Tech. 72:101-109, 2009. V

Research paper thumbnail of Observations of crypt neuron-like cells in the olfactory epithelium of a cartilaginous fish

Neuroscience Letters, 2006

A new receptor neuron (RN) type was recently described in bony fish olfactory epithelium (OE): th... more A new receptor neuron (RN) type was recently described in bony fish olfactory epithelium (OE): the crypt receptor neuron. This name is due to its main feature: the presence, at the apical part, of a deep invagination into which cilia protrude. The presence of this receptor neuron type is well documented in different species of bony fishes but it has never been described in cartilaginous fishes. In this study we demonstrate that crypt neuron-like cells are present in the olfactory epithelium of the elasmobranch Scyliorhinus canicula (Linnaeus, 1758). Histological observations allowed us to detect the presence of a few egg-shaped cells, characterized by a crypt like zone; ␣-tubulin immunoreactivity suggested the presence of cilia in the same area; fluorocrome conjugated lectin bindings suggested a distinctive mucus composition inside the presumptive crypt. The possible presence of crypt neuron-like cells in chondrichthyes would represent an interesting common feature between bony and cartilaginous fishes.

Research paper thumbnail of First detection of olfactory marker protein (OMP) immunoreactivity in the olfactory epithelium of a cartilaginous fish

Neuroscience Letters, 2007

Olfactory marker protein (OMP) is a protein expressed in the mature olfactory and vomeronasal neu... more Olfactory marker protein (OMP) is a protein expressed in the mature olfactory and vomeronasal neurons of many vertebrates, such as mammals, amphibians and bony fishes. Aim of this work was to investigate the OMP expression in the olfactory epithelium of the shark Scyliorhinus canicula (Linnaeus, 1758), by immunohistochemistry (IHC). Immunoreactivity was detected in the olfactory receptor neurons, in the crypt neurons and in the nerve fibers below the epithelium. Although very little is known about the OMP's function, its involvement in synaptogenesis, transduction cascade, neurogenesis and development of olfactory system has been suggested. The present work shows for the first time OMP's presence in a cartilaginous fish.

Research paper thumbnail of Presence and distribution of FMRFamide-like immunoreactivity in the cyprid of the barnacle Balanus amphitrite (Cirripedia, crustacea)

Microscopy Research and Technique, 2009

The presence and distribution of FMRFamide-like peptides (FLPs) in the cyprid larvae of the barna... more The presence and distribution of FMRFamide-like peptides (FLPs) in the cyprid larvae of the barnacle Balanus amphitrite were investigated using immunohistochemical methods. Barnacles are considered to be one of the most important constituents of animal fouling communities, and the cyprid stage is specialized for settlement and metamorphosis in to the sessile adult condition. FLPs immunoreactive (IR) neuronal cell bodies were detected in both the central and the peripheral nervous system. One bilateral group of neurons somata was immunodetected in the brain, and IR nerve fibers were observed in the neuropil area and optic lobes. Intense immunostaining was also observed in the frontal filament complex: frontal filament tracts leaving the optic lobes and projecting towards the compound eyes, swollen nerve endings in the frontal filament vesicles, and thin nerve endings in the external frontal filament. Thin IR nerve fibers were also present in the cement glands. Two pairs of neuronal cell bodies were immunodetected in the posterior ganglion; some of their axons appear to project to the cirri. FLPs IR neuronal cell bodies were also localized in the wall of the dilated midgut and in the narrow hindgut; their processes surround the gut wall and allow gut neurons to synapse with one another. Our data demonstrated the presence of FLPs IR substances in the barnacle cyprid. We hypothesize that these peptides act as integrators in the central nervous system, perform neuromuscular functions for thoracic limbs, trigger intestinal movements and, at the level of the frontal filament, play a neurosecretory role. Microsc. in Wiley InterScience (www.interscience.

Research paper thumbnail of Na + /K + ATPase immunoreactivity in olfactory epithelium of small-spotted catshark Scyliorhinus canicula (L.): possible presence of ion exchanging cells?

Journal of Fish Biology, 2006

ABSTRACT Na+/K+ATPase immunoreactivity in olfactory epithelium of Scyliorhinus canicula was local... more ABSTRACT Na+/K+ATPase immunoreactivity in olfactory epithelium of Scyliorhinus canicula was localized in well featured light large cells. It was distributed in the basolateral membranes as reported in the literature for typical ion exchanging cells.

Research paper thumbnail of Gut morphology and metallothionein immunoreactivity in Liza aurata from different heavy metal polluted environments

Italian Journal of Zoology, 2006

... DOI: 10.1080/11250000500502228 Sara Ferrando a * , Maria Maisano a , Vincenzo Parrino a , Tiz... more ... DOI: 10.1080/11250000500502228 Sara Ferrando a * , Maria Maisano a , Vincenzo Parrino a , Tiziana Ferrando b , Laura Girosi b & Grazia Tagliafierro b pages 7-14. ... 199612. Giacobbe, MG, Oliva, FD and Maimone, G. 1996. ...

Research paper thumbnail of Distribution of FMRFamide-like immunoreactivity in the nervous system of Lumbricus terrestris

Cell and Tissue Research, 1997

Research paper thumbnail of FMRFamide-like immunoreactivity in the sea-fan Eunicella cavolini (Cnidaria: Octocorallia)

Cell and Tissue Research, 2005

The presence of FMRFamide-related peptides (FaRPs) was investigated, by immunohistochemical metho... more The presence of FMRFamide-related peptides (FaRPs) was investigated, by immunohistochemical methods with a polyclonal FMRFamide antiserum, in the sea-fan Eunicella cavolini (Van Koch 1887), a representative of the cnidarians (octocorallians). The identification of FaRP-immunoreactive elements as neuronal cells and a nerve net was performed by double immunohistochemical methods with the monoclonal anti-beta-tubulin antibody. A strong and widely distributed FaRPs immunoreactivity was detected: FaRPsimmunoreactive nerve cells were observed among and underlying gastrodermal epithelial cells, epidermal cells lining tentacles, muscular septs and gonophores. A diffuse FaRPsimmunoreactive nerve net was also found between epithelia and mesoglea and in the stalk of the gonophore. These results improve our knowledge of the gorgonian nervous system and demonstrate that most of the immunoreactive cells belong to neural elements.

Research paper thumbnail of Gamma-aminobutyric acid and related molecules in the sea fan Eunicella cavolini (Cnidaria: Octocorallia): a biochemical and immunohistochemical approach

Cell and Tissue Research, 2007

The aim of this study has been the biochemical demonstration of the presence of γ-aminobutyric ac... more The aim of this study has been the biochemical demonstration of the presence of γ-aminobutyric acid (GABA) in the Mediterranean sea fan Eunicella cavolini by means of high-performance liquid chromatography, and the description of the distribution pattern of GABA and its related molecules, glutamic acid decarboxylase (GAD), vesicular GABA transporter (VGAT) and one of the GABA receptors (GABA B R) by immunohistochemical methods. The interrelationships of GABA, GAD and GABA receptor immunoreactivity have been established by using doubleimmunohistochemical methods and confocal microscopy.

Research paper thumbnail of Distribution of choline acetyltransferase immunopositive structures in the rat brainstem

Research paper thumbnail of BRIEF COMMUNICATIONS Na+/K+ ATPase immunoreactivity in olfactory epithelium of small-spotted catshark Scyliorhinus canicula (L.): possible presence of ion exchanging cells?

Research paper thumbnail of New records of blonde ray ( Raja brachyura) from the Ligurian Sea

Marine Biodiversity Records, 2008

ABSTRACT The first confirmed records of two blonde rays (Raja brachyura) in the Ligurian Sea for ... more ABSTRACT The first confirmed records of two blonde rays (Raja brachyura) in the Ligurian Sea for 140 years are described. Two specimens were caught off Cogoleto (western Ligurian Sea) and they were deposited in the Civic Museum of Natural History ‘Giacomo Doria’ of Genoa. Morphometric and meristic features have been reported.

Research paper thumbnail of Il sistema GABAergico in Eunicella cavolini

Research paper thumbnail of Immunohistochemical FMRFamide-like distribution in the cyprid of the barnacle Balanus amphitrite

Research paper thumbnail of PACAP in developing sensory and peripheral organs of the zebrafish, Danio rerio

European journal of histochemistry : EJH

The anatomical distribution of PACAP-like immunoreactivity was investigated in sensory and periph... more The anatomical distribution of PACAP-like immunoreactivity was investigated in sensory and peripheral organs of the zebrafish, Danio rerio, during the pharyngula, hatching and larval periods, by using indirect immunofluorescence methods. First PACAP-like immunoreactive (ir) elements appeared during the pharyngula period, at 24 hours post fertilization (hpf), within the most superficial layer of the retina and the dorsal aorta. At 48 hpf, additional ir cells were found in the olfactory placode and esophagus. At 72 hpf (hatching period), PACAP-like immunoreactivity was first detected in the ganglion cell layer of the retina, the otic sensory epithelium, pharyngeal arches, swim bladder and pancreatic progenitor cells. During day 5 of larval development, new groups of ir cells appeared in the liver, whereas no ir elements were observed in the olfactory placode. Subsequently, at day 13 of larval development, additional ir elements were found for the first time in some gut epithelial cell...

Research paper thumbnail of First record of albinism in the deep-water shark Dalatias licha

Marine Biodiversity Records, 2008

ABSTRACT The first case of albinism in the kitefin shark, Dalatias licha, is reported here throug... more ABSTRACT The first case of albinism in the kitefin shark, Dalatias licha, is reported here through the study of one specimen caught by professional bottom trawl in the Gulf of Genoa, Ligurian Sea. The albino surface was quantified and morphometric and meristic characteristics were compared with those of normal specimens of the same sex and equivalent length, caught within the same area. The shark body presented a partial reduction in pigmentation and no morphometric differences between the albino specimen and the normal individuals were observed.

Research paper thumbnail of Apoptosis, cell proliferation and serotonin immunoreactivity in gut of Liza aurata from natural heavy metal polluted environments: preliminary observations

European Journal of Histochemistry, 2005

Research paper thumbnail of Presence and distribution of serotonin immunoreactivity in the cyprids of the barnacle Balanus amphitrite

European Journal of Histochemistry, 2005

The presence and distribution of FMRFamide-like peptides (FLPs) in the cyprid larvae of the barna... more The presence and distribution of FMRFamide-like peptides (FLPs) in the cyprid larvae of the barnacle Balanus amphitrite were investigated using immunohistochemical methods. Barnacles are considered to be one of the most important constituents of animal fouling communities, and the cyprid stage is specialized for settlement and metamorphosis in to the sessile adult condition. FLPs immunoreactive (IR) neuronal cell bodies were detected in both the central and the peripheral nervous system. One bilateral group of neurons somata was immunodetected in the brain, and IR nerve fibers were observed in the neuropil area and optic lobes. Intense immunostaining was also observed in the frontal filament complex: frontal filament tracts leaving the optic lobes and projecting towards the compound eyes, swollen nerve endings in the frontal filament vesicles, and thin nerve endings in the external frontal filament. Thin IR nerve fibers were also present in the cement glands. Two pairs of neuronal cell bodies were immunodetected in the posterior ganglion; some of their axons appear to project to the cirri. FLPs IR neuronal cell bodies were also localized in the wall of the dilated midgut and in the narrow hindgut; their processes surround the gut wall and allow gut neurons to synapse with one another. Our data demonstrated the presence of FLPs IR substances in the barnacle cyprid. We hypothesize that these peptides act as integrators in the central nervous system, perform neuromuscular functions for thoracic limbs, trigger intestinal movements and, at the level of the frontal filament, play a neurosecretory role. Microsc. Res. Tech. 72:101-109, 2009. V

Research paper thumbnail of Observations of crypt neuron-like cells in the olfactory epithelium of a cartilaginous fish

Neuroscience Letters, 2006

A new receptor neuron (RN) type was recently described in bony fish olfactory epithelium (OE): th... more A new receptor neuron (RN) type was recently described in bony fish olfactory epithelium (OE): the crypt receptor neuron. This name is due to its main feature: the presence, at the apical part, of a deep invagination into which cilia protrude. The presence of this receptor neuron type is well documented in different species of bony fishes but it has never been described in cartilaginous fishes. In this study we demonstrate that crypt neuron-like cells are present in the olfactory epithelium of the elasmobranch Scyliorhinus canicula (Linnaeus, 1758). Histological observations allowed us to detect the presence of a few egg-shaped cells, characterized by a crypt like zone; ␣-tubulin immunoreactivity suggested the presence of cilia in the same area; fluorocrome conjugated lectin bindings suggested a distinctive mucus composition inside the presumptive crypt. The possible presence of crypt neuron-like cells in chondrichthyes would represent an interesting common feature between bony and cartilaginous fishes.

Research paper thumbnail of First detection of olfactory marker protein (OMP) immunoreactivity in the olfactory epithelium of a cartilaginous fish

Neuroscience Letters, 2007

Olfactory marker protein (OMP) is a protein expressed in the mature olfactory and vomeronasal neu... more Olfactory marker protein (OMP) is a protein expressed in the mature olfactory and vomeronasal neurons of many vertebrates, such as mammals, amphibians and bony fishes. Aim of this work was to investigate the OMP expression in the olfactory epithelium of the shark Scyliorhinus canicula (Linnaeus, 1758), by immunohistochemistry (IHC). Immunoreactivity was detected in the olfactory receptor neurons, in the crypt neurons and in the nerve fibers below the epithelium. Although very little is known about the OMP's function, its involvement in synaptogenesis, transduction cascade, neurogenesis and development of olfactory system has been suggested. The present work shows for the first time OMP's presence in a cartilaginous fish.

Research paper thumbnail of Presence and distribution of FMRFamide-like immunoreactivity in the cyprid of the barnacle Balanus amphitrite (Cirripedia, crustacea)

Microscopy Research and Technique, 2009

The presence and distribution of FMRFamide-like peptides (FLPs) in the cyprid larvae of the barna... more The presence and distribution of FMRFamide-like peptides (FLPs) in the cyprid larvae of the barnacle Balanus amphitrite were investigated using immunohistochemical methods. Barnacles are considered to be one of the most important constituents of animal fouling communities, and the cyprid stage is specialized for settlement and metamorphosis in to the sessile adult condition. FLPs immunoreactive (IR) neuronal cell bodies were detected in both the central and the peripheral nervous system. One bilateral group of neurons somata was immunodetected in the brain, and IR nerve fibers were observed in the neuropil area and optic lobes. Intense immunostaining was also observed in the frontal filament complex: frontal filament tracts leaving the optic lobes and projecting towards the compound eyes, swollen nerve endings in the frontal filament vesicles, and thin nerve endings in the external frontal filament. Thin IR nerve fibers were also present in the cement glands. Two pairs of neuronal cell bodies were immunodetected in the posterior ganglion; some of their axons appear to project to the cirri. FLPs IR neuronal cell bodies were also localized in the wall of the dilated midgut and in the narrow hindgut; their processes surround the gut wall and allow gut neurons to synapse with one another. Our data demonstrated the presence of FLPs IR substances in the barnacle cyprid. We hypothesize that these peptides act as integrators in the central nervous system, perform neuromuscular functions for thoracic limbs, trigger intestinal movements and, at the level of the frontal filament, play a neurosecretory role. Microsc. in Wiley InterScience (www.interscience.

Research paper thumbnail of Na + /K + ATPase immunoreactivity in olfactory epithelium of small-spotted catshark Scyliorhinus canicula (L.): possible presence of ion exchanging cells?

Journal of Fish Biology, 2006

ABSTRACT Na+/K+ATPase immunoreactivity in olfactory epithelium of Scyliorhinus canicula was local... more ABSTRACT Na+/K+ATPase immunoreactivity in olfactory epithelium of Scyliorhinus canicula was localized in well featured light large cells. It was distributed in the basolateral membranes as reported in the literature for typical ion exchanging cells.

Research paper thumbnail of Gut morphology and metallothionein immunoreactivity in Liza aurata from different heavy metal polluted environments

Italian Journal of Zoology, 2006

... DOI: 10.1080/11250000500502228 Sara Ferrando a * , Maria Maisano a , Vincenzo Parrino a , Tiz... more ... DOI: 10.1080/11250000500502228 Sara Ferrando a * , Maria Maisano a , Vincenzo Parrino a , Tiziana Ferrando b , Laura Girosi b & Grazia Tagliafierro b pages 7-14. ... 199612. Giacobbe, MG, Oliva, FD and Maimone, G. 1996. ...

Research paper thumbnail of Distribution of FMRFamide-like immunoreactivity in the nervous system of Lumbricus terrestris

Cell and Tissue Research, 1997

Research paper thumbnail of FMRFamide-like immunoreactivity in the sea-fan Eunicella cavolini (Cnidaria: Octocorallia)

Cell and Tissue Research, 2005

The presence of FMRFamide-related peptides (FaRPs) was investigated, by immunohistochemical metho... more The presence of FMRFamide-related peptides (FaRPs) was investigated, by immunohistochemical methods with a polyclonal FMRFamide antiserum, in the sea-fan Eunicella cavolini (Van Koch 1887), a representative of the cnidarians (octocorallians). The identification of FaRP-immunoreactive elements as neuronal cells and a nerve net was performed by double immunohistochemical methods with the monoclonal anti-beta-tubulin antibody. A strong and widely distributed FaRPs immunoreactivity was detected: FaRPsimmunoreactive nerve cells were observed among and underlying gastrodermal epithelial cells, epidermal cells lining tentacles, muscular septs and gonophores. A diffuse FaRPsimmunoreactive nerve net was also found between epithelia and mesoglea and in the stalk of the gonophore. These results improve our knowledge of the gorgonian nervous system and demonstrate that most of the immunoreactive cells belong to neural elements.

Research paper thumbnail of Gamma-aminobutyric acid and related molecules in the sea fan Eunicella cavolini (Cnidaria: Octocorallia): a biochemical and immunohistochemical approach

Cell and Tissue Research, 2007

The aim of this study has been the biochemical demonstration of the presence of γ-aminobutyric ac... more The aim of this study has been the biochemical demonstration of the presence of γ-aminobutyric acid (GABA) in the Mediterranean sea fan Eunicella cavolini by means of high-performance liquid chromatography, and the description of the distribution pattern of GABA and its related molecules, glutamic acid decarboxylase (GAD), vesicular GABA transporter (VGAT) and one of the GABA receptors (GABA B R) by immunohistochemical methods. The interrelationships of GABA, GAD and GABA receptor immunoreactivity have been established by using doubleimmunohistochemical methods and confocal microscopy.

Research paper thumbnail of Distribution of choline acetyltransferase immunopositive structures in the rat brainstem

Research paper thumbnail of BRIEF COMMUNICATIONS Na+/K+ ATPase immunoreactivity in olfactory epithelium of small-spotted catshark Scyliorhinus canicula (L.): possible presence of ion exchanging cells?

Research paper thumbnail of New records of blonde ray ( Raja brachyura) from the Ligurian Sea

Marine Biodiversity Records, 2008

ABSTRACT The first confirmed records of two blonde rays (Raja brachyura) in the Ligurian Sea for ... more ABSTRACT The first confirmed records of two blonde rays (Raja brachyura) in the Ligurian Sea for 140 years are described. Two specimens were caught off Cogoleto (western Ligurian Sea) and they were deposited in the Civic Museum of Natural History ‘Giacomo Doria’ of Genoa. Morphometric and meristic features have been reported.