Lorena Maria Alves de souza (original) (raw)

Papers by Lorena Maria Alves de souza

American Journal of Dentistry, 2008

To evaluate the cytotoxic effects of different concentrations of Chlorhexidine (Chx) to the odont... more To evaluate the cytotoxic effects of different concentrations of Chlorhexidine (Chx) to the odontoblast cell line MDPC-23. The odontoblast-like cells were seeded (30,000 cells/cm2) in 60 wells of 24-well dishes and then incubated in contact with the following experimental and control solutions: Group 1: 0.0024% Chx; Group 2: 0.004% Chx; Group 3: 0.02% Chx; Group 4: Phosphate buffer saline solution (PBS, negative control); and Group 5: 0.06% H2O2 (positive control). Cell metabolic activity was measured by MTT assay and the cell morphology was analyzed by SEM. The cytotoxic effects of Chx are dose-dependent. The reduction in the cell metabolism for Groups 1, 2, and 3 was 24.8%, 29.9% and 70.8%, respectively. No statistical difference was observed between the Groups 1 and 2 in which no significant cell morphology changes occurred. Consequently, it was concluded that 0.02% Chx solution presents high cytotoxicity to the odontoblast-like cells MDPC-23. On the other hand, 0.0024% and 0.004% Chx causes slight cytopathic effects to the cultured cells.

11O Congresso Internacional Da Rede Unida, Apr 30, 2014

Introducao: O Ministerio da Saude preconiza que a atencao a mulher no ciclo gravidico puerperal s... more Introducao: O Ministerio da Saude preconiza que a atencao a mulher no ciclo gravidico puerperal seja marcada pela humanizacao e qualidade da assistencia prestada. Nesse contexto, faz-se necessario que os profissionais incorporem condutas respaldadas pelo acolhimento, possibilitando o acesso e a acessibilidade das gestantes aos servicos de saude, com acoes prioritarias que perpassem todos os niveis de atencao a saude. Objetivo: Avaliar a assistencia pre-natal em uma Unidade Basica de Saude segundo a perspectiva das gestantes. Metodologia: Estudo exploratorio, qualitativo, realizado com 12 gestantes que faziam acompanhamento pre-natal em uma Unidade Basica de Saude da Familia de Cascavel – CE, no periodo de maio e junho de 2013. A coleta de dados foi atraves de uma entrevista semi-estruturada. Os aspectos eticos da pesquisa foram respeitados conforme a Resolucao n.o466/12 do Conselho Nacional de Saude. Resultados: Na categoria Acessibilidade e acolhimento observamos que as gestantes procuram o servico de saude para fazer o acompanhamento pre-natal e enfrentam dificuldades para serem atendidas e dar seguimento as etapas necessarias a assistencia de qualidade. Alem disso, a grande demanda e o pequeno numero ineficiente de profissionais para atender essa populacao tambem interferem na qualidade da assistencia. Na categoria Integralidade do atendimento que e entendida como um conjunto articulado de acoes e servicos de saude em todos os niveis de complexidade do sistema observa-se que varios procedimentos nao sao realizados, sendo estes desejados e reconhecidos pelas gestantes como acoes integrante do acompanhamento pre-natal, tornando-se essenciais para a qualidade do atendimento. Na categoria Humanizacao da assistencia pre-natal que significa promover saude atendendo melhor o usuario e a producao de respeito mutuo nas relacoes interpessoais, foi relatado situacoes que esse processo nao foi realizado, pois a acolhida humanizada da gestante, deve ser proporcionada desde a sua chegada a unidade, envolvendo e responsabilizando os cuidados e atencao a todos os profissionais da equipe. Conclusao: Concluimos que a assistencia pre-natal ainda e caracterizada como uma consulta ambulatorial com solicitacao de exames. Portanto, faz-se necessario que os profissionais de saude desenvolvam estrategias que visem a promocao da saude materno-infantil, como acoes de educacao em saude podendo tornar as usuarias protagonistas no processo de cuidado e assistencia a saude.

Virtu Direito E Humanismo, Jan 19, 2014

This research proposes a critical analysis of student migration in the current modern society pro... more This research proposes a critical analysis of student migration in the current modern society produces of merchandise, discussing the coming of African students for the Brazilian universities, in particular, the Federal University of Goiás (UFG), the Pontificia Catholic University of Goiás (PUC-GO) and the State University of Goiás (UEG) from the program of Student Agreement Undergraduate and Postgraduate (PEC-G and PG). This program stands out as an important educational cooperation agreement established between Brazil and Third World countries, among them the African Portuguese-speaking countries. We investigate and propose a discussion about the African migrant student, under the base of the qualification of the labor force and value-dissociation and prerogatives to the critical value from the racial issue as a peculiarity. In modern society crisis, the student mobility is seen as a government alternative to the economic and political development of the State, therefore, the student migration is a particular phenomenon of mobility of the labor force generated by the modernization process. The mobility of students among developing countries has been an ever-present reality and encourages us to think of the migrant from the perspective of international agreements, and the race for qualification which becomes a key requirement in producer goods system. Reflect on the current situation in which the migrant is inserting on the temporary conditions, of the future qualified labor, such as the student migrant. Through literature review, collecting data from official agencies, on the universities destination for African migrants students and interviews with the same, we discussed the social representation of African migrants in the "destination" land, and the consequences of racism, treated as the not desire "foreign" or "not accepted" or African on generalized sense, prejudiced and discriminatory.

Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, 2015

Stromal interaction molecule 1 (STIM1) senses depletion of ER-Ca 2+ store and clusters in ER-PM j... more Stromal interaction molecule 1 (STIM1) senses depletion of ER-Ca 2+ store and clusters in ER-PM junctions where it associates with and gates Ca 2+ influx channels, Orai1 and TRPC1. Clustering of TRPC1 with STIM1 and Orai1 in these junctions is critical since Orai1-mediated Ca 2+ entry triggers surface expression of TRPC1 while STIM1 gates the channel. Thus, plasma membrane function of TRPC1 depends on the delivery of the channel to the sites where STIM1 puncta are formed. This study examines intracellular trafficking mechanism(s) that determine plasma membrane expression and function of TRPC1 in cells where Orai1 and TRPC1 are endogenously expressed and contribute to Ca 2+ entry. We report that TRPC1 is internalized by Arf6-dependent pathway, sorted to Rab5-containing early endosomes, and trafficked to ER-PM junctions by Rab4-dependent fast recycling. Overexpression of Arf6, or Rab5, but not the respective dominant negative mutants, induced retention of TRPC1 in early endosomes and suppressed TRPC1 function. Notably, cells expressing Arf6 or Rab5 displayed an inwardly rectifying I CRAC current that is mediated by Orai1 instead of TRPC1-associated I SOC , demonstrating that Orai1 function was not altered. Importantly, expression of Rab4, but not STIM1, with Rab5 rescued surface expression and function of TRPC1, restoring generation of I SOC. Together, these data demonstrate that trafficking via fast recycling endosomes determines TRPC1-STIM1 clustering within ER-PM junctions following ER-Ca 2+ store depletion which is critical for the surface expression and function of the channel. Ca 2+ influx mediated by TRPC1 modifies Ca 2+-dependent physiological response of cells.

Science Signaling, 2015

STIM2 increases physiological responses to agonist stimulation by promoting clustering of STIM1 a... more STIM2 increases physiological responses to agonist stimulation by promoting clustering of STIM1 and activation of Orai1.

Handbook of Experimental Pharmacology, 2014

The TRP-canonical (TRPC) subfamily, which consists of seven members (TRPC1-TRPC7), are Ca(2+)-per... more The TRP-canonical (TRPC) subfamily, which consists of seven members (TRPC1-TRPC7), are Ca(2+)-permeable cation channels that are activated in response to receptor-mediated PIP2 hydrolysis via store-dependent and store-independent mechanisms. These channels are involved in a variety of physiological functions in different cell types and tissues. Of these, TRPC6 has been linked to a channelopathy resulting in human disease. Two key players of the store-dependent regulatory pathway, STIM1 and Orai1, interact with some TRPC channels to gate and regulate channel activity. The Ca(2+) influx mediated by TRPC channels generates distinct intracellular Ca(2+) signals that regulate downstream signaling events and consequent cell functions. This requires localization of TRPC channels in specific plasma membrane microdomains and precise regulation of channel function which is coordinated by various scaffolding, trafficking, and regulatory proteins.

Cell Calcium, 2014

Highlights Ca 2+-permeable TRPC channels contribute to various cellular functions TRPC channel tr... more Highlights Ca 2+-permeable TRPC channels contribute to various cellular functions TRPC channel trafficking is a key determinant of their plasma membrane function This review discusses the mechanisms and pathways regulating TRPC trafficking The topics discussed are endocytic, recycling, as well as exocytic mechanisms *Highlights (for review) A c c e p t e d M a n u s c r i p t 1 Trafficking mechanisms and regulation of TRPC Channels.

PLoS ONE, 2014

Phospholipase D (PLD) has been implicated in many cellular functions, such as vesicle trafficking... more Phospholipase D (PLD) has been implicated in many cellular functions, such as vesicle trafficking, exocytosis, differentiation, and proliferation. The aim of this study was to characterize the role of PLD in HSY cells, a human cell line originating from the intercalated duct of the parotid gland. As the function and intracellular localization of PLD varies according to cell type, initially, the intracellular localization of PLD1 and PLD2 was determined. By immunofluorescence, PLD1 and PLD2 both showed a punctate cytoplasmic distribution with extensive co-localization with TGN-46. PLD1 was also found in the nucleus, while PLD2 was associated with the plasma membrane. Treatment of cells with the primary alcohol 1-butanol inhibits the hydrolysis of phosphatidylcoline by PLD thereby suppressing phosphatidic acid (PA) production. In untreated HSY cells, there was only a slight co-localization of PLD with the clathrin coated vesicles. When HSY cells were incubated with 1butanol the total number of clathrin coated vesicles increased, especially in the juxtanuclear region and the co-localization of PLD with the clathrin coated vesicles was augmented. Transmission electron microscopy confirmed that the number of Golgi-associated coated vesicles was greater. Treatment with 1-butanol also affected the Golgi apparatus, increasing the volume of the Golgi saccules. The decrease in PA levels after treatment with 1-butanol likewise resulted in an accumulation of enlarged lysosomes in the perinuclear region. Therefore, in HSY cells PLD appears to be involved in the formation of Golgi associated clathrin coated vesicles as well as in the structural maintenance of the Golgi apparatus.

Biochemical Journal, 2014

SOCE (store-operated Ca2+ entry) is mediated via specific plasma membrane channels in response to... more SOCE (store-operated Ca2+ entry) is mediated via specific plasma membrane channels in response to ER (endoplasmic reticulum) Ca2+ store depletion. This route of Ca2+ entry is central to the dynamic interplay between Ca2+ and cAMP signalling in regulating the activity of Ca2+-sensitive adenylate cyclase isoforms (AC1, AC5, AC6 and AC8). Two proteins have been identified as key components of SOCE: STIM1 (stromal interaction molecule 1), which senses ER Ca2+ store content and translocates to the plasma membrane upon store depletion, where it then activates Orai1, the pore-forming component of the CRAC (Ca2+ release-activated Ca2+) channel. Previous studies reported that co-expression of STIM1 and Orai1 in HEK-293 (human embryonic kidney 293) cells enhances Ca2+-stimulated AC8 activity and that AC8 and Orai1 directly interact to enhance this regulation. Nonetheless, the additional involvement of TRPC (transient receptor potential canonical) channels in SOCE has also been proposed. In th...

Biophysical Journal, 2015

Acolhendo a Alfabetização nos Países de Língua Portuguesa, 2010

O conto através do teatro como meio alfabetizador e transmissor das culturas e histórias africana... more O conto através do teatro como meio alfabetizador e transmissor das culturas e histórias africanas. Revista ACOALFAplp:

Revista Brasileira de Ciência Veterinária, 2005

Ateliê Geográfico, 2013

Resumo O objetivo deste artigo é apresentar os resultados teóricos e metodológicos de uma propost... more Resumo O objetivo deste artigo é apresentar os resultados teóricos e metodológicos de uma proposta de trabalho de campo integrado, que envolveram disciplinas da estrutura curricular do curso de Licenciatura em Geografia. O trabalho também é composto por uma reflexão acerca da importância deste meio de ensino na integração teoria e prática e de uma análise integrada, contemplando aspectos sociais e ambientais que se interrelacionam no espaço geográfico. A proposta foi desenvolvida com os alunos do 2° e 3° anos do curso de Geografia da Universidade Estadual de Goiás-Unidade de Minaçu. As disciplinas contempladas foram a Geomorfologia, a Biogeografia, a Geografia Cultural, a Geografia Agrária e a Cartografia Temática, tendo como campo o Parque Nacional Chapada dos Veadeiros e região. Os procedimentos se desenvolveram em duas etapas, uma em sala de aula, onde foram feitas leituras de textos, discussão dos temas a serem desenvolvidos e elaboração dos mapas temáticos, e um segundo momento no campo, onde se procederam à coleta de dados, descrições e análises dos aspectos culturais, agrários e físicos do parque e área do entorno.

Ateliê Geográfico, 2009

Este artigo propõe fazer uma discussão sobre as categorias gênero, raça, espaço e poder na realid... more Este artigo propõe fazer uma discussão sobre as categorias gênero, raça, espaço e poder na realidade das relações sociais. O poder se manifesta tanto de maneira visível quanto invisível dependendo das estratégias do grupo que lhe detém e, em nome do poder político, econômico e cultural. A segregação espacial a que alguns grupos são submetidos é um reflexo da predominância de privilégios de uma classe dominante ao longo da formação social brasileira. A partir de autores/as pesquisadores/as das questões de gênero, feminismo, raça e espaço realizamos uma reflexão sobre o interesse da Geografia Cultural sobre estas temáticas, bem como sobre as relações de poder de grupos dominantes sobre os dominados e a maneira como a herança escravocrata colonial assegurou índices alarmantes de desemprego e subemprego da população negra no mercado de trabalho. Palavras-chave: relações de poder, gênero, espaço, raça Résumé Cet article propose faire une discussion sur les categories genre, race, espace et pouvoir dans les relations sociales. Le pouvoir se manifeste visiblement ou invisiblement, ça dépend des stratégies des groupes, avec le nom du pouvoir politique, econonomique et culturel. La segregation spatial qui quelques groupes souffrent est un reflet de la Ateliê Geográfico Goiânia-GO v. 3, n. 1 abr/2009 p.97-110 página 98

Ciência Animal Brasileira, 2010

Fiscal federal agropecuário do Ministério da Agricultura, Pecuária e Abastecimento 8. Professor d... more Fiscal federal agropecuário do Ministério da Agricultura, Pecuária e Abastecimento 8. Professor de Doenças Infecciosas DVP/FV/UFPel 9. Professor associado da Universidade Federal de Pelotas (UFPel). RESUMO Considerando a importância do VLFe na clínica felina, assim como a possível disseminação do agente a partir de um felino portador sintomático ou assintomático, o estudo tem como objetivo verificar a frequência de viremia pelo VLFe em felinos residentes em Pelotas e Rio Grande, municípios situados na região sul do Brasil. Para isso foi coletado sangue de 120 animais semi-domiciliados para a detecção do retrovírus através da técnica de imunofluorescência indireta (IFI). Detectou-se a viremia em 38,3% (46/120) dos animais estudados, representando uma frequência maior em relação a outros estudos realizados no Brasil, o que confirma a importância deste agente na região estudada.

Journal of Virological Methods, 2009

Meningoencephalitis caused by Bovine herpesvirus type 5 (BoHV-5) is responsible for heavy economi... more Meningoencephalitis caused by Bovine herpesvirus type 5 (BoHV-5) is responsible for heavy economic losses in the cattle industry. As in other Alphaherpesviruses, the envelope glycoprotein IV (gD), which mediates penetration into host cells, is one of the major candidate antigens for a recombinant vaccine, since it induces a strong and persistent immune response. The DNA coding for a truncated form of BoHV-5 gD (tgD) has been cloned into the Pichia pastoris expression vector pPICZalphaB to allow protein secretion into the medium. After induction with methanol, a approximately 55kDa protein was obtained. Enzyme deglycosylation with Endo H showed a smaller size band in SDS-PGAE, with approximately 50kDa, suggesting that tgD has N-linked oligosaccharides and that it is not hyperglycosylated. The approximately 55kDa protein was recognized by several polyclonal antibodies, including polyclonal antibody anti-tgD and polyclonal antibodies of different animal species immunized with BoHV-5 and BoHV-1. This is the first report of BoHV-5 gD expression in yeast. It was shown that the recombinant truncated form of BoHV-5 gD has antigenic and immunogenic properties similar to the native BoHV-5 gD. Expression of tgD as a secreted protein allows simple and inexpensive purification methods that can be used for further studies to evaluate its immunogenicity in cattle.

Journal of Histochemistry & Cytochemistry, 2012

The current study examined the role of PLD2 in the maintenance of mast cell structure. Phospholip... more The current study examined the role of PLD2 in the maintenance of mast cell structure. Phospholipase D (PLD) catalyzes hydrolysis of phosphatidylcholine to produce choline and phosphatidic acid (PA). PLD has two isoforms, PLD1 and PLD2, which vary in expression and localization depending on the cell type. The mast cell line RBL-2H3 was transfected to overexpress catalytically active (PLD2CA) and inactive (PLD2CI) forms of PLD2. The results of this study show that PLD2CI cells have a distinct star-shaped morphology, whereas PLD2CA and RBL-2H3 cells are spindle shaped. In PLD2CI cells, the Golgi complex was also disorganized with dilated cisternae, and more Golgi-associated vesicles were present as compared with the PLD2CA and RBL-2H3 cells. Treatment with exogenous PA led to the restoration of the wild-type Golgi complex phenotype in PLD2CI cells. Conversely, treatment of RBL-2H3 and PLD2CA cells with 1% 1-Butanol led to a disruption of the Golgi complex. The distribution of acidic c...

Journal of Biomedical Materials Research Part B: Applied Biomaterials, 2009

This study evaluated the cytotoxic effects of a carbamide peroxide (CP) bleaching gel at differen... more This study evaluated the cytotoxic effects of a carbamide peroxide (CP) bleaching gel at different concentrations on odontoblast-like cells. Immortalized cells of the MDPC-23 cell line (30,000 cells/cm(2)) were incubated for 48 h. The bleaching gel was diluted in DMEM culture medium originating extracts with different CP concentrations. The amount (microg/mL) of hydrogen peroxide (H(2)O(2)) released from each extract was measured by the leukocrystal violet/horseradish peroxidase enzyme assay. Five groups (n = 10) were formed according to the CP concentration in the extracts: G1-DMEM (control); G2-0.0001% CP (0.025 microg/mL H(2)O(2)); G3-0.001% CP (0.43 microg/mL H(2)O(2)); G4-0.01% CP (2.21 microg/mL H(2)O(2)); and G5-0.1% CP (29.74 microg/mL H(2)O(2)). MDPC-23 cells were exposed to the bleaching gel extracts for 60 min and cell metabolism was evaluated by the MTT assay. Data were analyzed statistically by one-way ANOVA and Tukey's test (alpha = 0.05). Cell morphology was examined by scanning electron microscopy. The percentages of viable cells were as follows: G1, 100%; G2, 89.41%; G3, 82.4%; G4, 61.5%; and G5, 23.0%. G2 and G3 did not differ significantly (p > 0.05) from G1. The most severe cytotoxic effects were observed in G3 and G4. In conclusion, even at low concentrations, the CP gel extracts presented cytotoxic effects. This cytotoxicity was dose-dependent, and the 0.1% CP concentration caused the most intense cytopathic effects to the MDPC-23 cells.

American Journal of Dentistry, 2008

To evaluate the cytotoxic effects of different concentrations of Chlorhexidine (Chx) to the odont... more To evaluate the cytotoxic effects of different concentrations of Chlorhexidine (Chx) to the odontoblast cell line MDPC-23. The odontoblast-like cells were seeded (30,000 cells/cm2) in 60 wells of 24-well dishes and then incubated in contact with the following experimental and control solutions: Group 1: 0.0024% Chx; Group 2: 0.004% Chx; Group 3: 0.02% Chx; Group 4: Phosphate buffer saline solution (PBS, negative control); and Group 5: 0.06% H2O2 (positive control). Cell metabolic activity was measured by MTT assay and the cell morphology was analyzed by SEM. The cytotoxic effects of Chx are dose-dependent. The reduction in the cell metabolism for Groups 1, 2, and 3 was 24.8%, 29.9% and 70.8%, respectively. No statistical difference was observed between the Groups 1 and 2 in which no significant cell morphology changes occurred. Consequently, it was concluded that 0.02% Chx solution presents high cytotoxicity to the odontoblast-like cells MDPC-23. On the other hand, 0.0024% and 0.004% Chx causes slight cytopathic effects to the cultured cells.

11O Congresso Internacional Da Rede Unida, Apr 30, 2014

Introducao: O Ministerio da Saude preconiza que a atencao a mulher no ciclo gravidico puerperal s... more Introducao: O Ministerio da Saude preconiza que a atencao a mulher no ciclo gravidico puerperal seja marcada pela humanizacao e qualidade da assistencia prestada. Nesse contexto, faz-se necessario que os profissionais incorporem condutas respaldadas pelo acolhimento, possibilitando o acesso e a acessibilidade das gestantes aos servicos de saude, com acoes prioritarias que perpassem todos os niveis de atencao a saude. Objetivo: Avaliar a assistencia pre-natal em uma Unidade Basica de Saude segundo a perspectiva das gestantes. Metodologia: Estudo exploratorio, qualitativo, realizado com 12 gestantes que faziam acompanhamento pre-natal em uma Unidade Basica de Saude da Familia de Cascavel – CE, no periodo de maio e junho de 2013. A coleta de dados foi atraves de uma entrevista semi-estruturada. Os aspectos eticos da pesquisa foram respeitados conforme a Resolucao n.o466/12 do Conselho Nacional de Saude. Resultados: Na categoria Acessibilidade e acolhimento observamos que as gestantes procuram o servico de saude para fazer o acompanhamento pre-natal e enfrentam dificuldades para serem atendidas e dar seguimento as etapas necessarias a assistencia de qualidade. Alem disso, a grande demanda e o pequeno numero ineficiente de profissionais para atender essa populacao tambem interferem na qualidade da assistencia. Na categoria Integralidade do atendimento que e entendida como um conjunto articulado de acoes e servicos de saude em todos os niveis de complexidade do sistema observa-se que varios procedimentos nao sao realizados, sendo estes desejados e reconhecidos pelas gestantes como acoes integrante do acompanhamento pre-natal, tornando-se essenciais para a qualidade do atendimento. Na categoria Humanizacao da assistencia pre-natal que significa promover saude atendendo melhor o usuario e a producao de respeito mutuo nas relacoes interpessoais, foi relatado situacoes que esse processo nao foi realizado, pois a acolhida humanizada da gestante, deve ser proporcionada desde a sua chegada a unidade, envolvendo e responsabilizando os cuidados e atencao a todos os profissionais da equipe. Conclusao: Concluimos que a assistencia pre-natal ainda e caracterizada como uma consulta ambulatorial com solicitacao de exames. Portanto, faz-se necessario que os profissionais de saude desenvolvam estrategias que visem a promocao da saude materno-infantil, como acoes de educacao em saude podendo tornar as usuarias protagonistas no processo de cuidado e assistencia a saude.

Virtu Direito E Humanismo, Jan 19, 2014

This research proposes a critical analysis of student migration in the current modern society pro... more This research proposes a critical analysis of student migration in the current modern society produces of merchandise, discussing the coming of African students for the Brazilian universities, in particular, the Federal University of Goiás (UFG), the Pontificia Catholic University of Goiás (PUC-GO) and the State University of Goiás (UEG) from the program of Student Agreement Undergraduate and Postgraduate (PEC-G and PG). This program stands out as an important educational cooperation agreement established between Brazil and Third World countries, among them the African Portuguese-speaking countries. We investigate and propose a discussion about the African migrant student, under the base of the qualification of the labor force and value-dissociation and prerogatives to the critical value from the racial issue as a peculiarity. In modern society crisis, the student mobility is seen as a government alternative to the economic and political development of the State, therefore, the student migration is a particular phenomenon of mobility of the labor force generated by the modernization process. The mobility of students among developing countries has been an ever-present reality and encourages us to think of the migrant from the perspective of international agreements, and the race for qualification which becomes a key requirement in producer goods system. Reflect on the current situation in which the migrant is inserting on the temporary conditions, of the future qualified labor, such as the student migrant. Through literature review, collecting data from official agencies, on the universities destination for African migrants students and interviews with the same, we discussed the social representation of African migrants in the "destination" land, and the consequences of racism, treated as the not desire "foreign" or "not accepted" or African on generalized sense, prejudiced and discriminatory.

Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, 2015

Stromal interaction molecule 1 (STIM1) senses depletion of ER-Ca 2+ store and clusters in ER-PM j... more Stromal interaction molecule 1 (STIM1) senses depletion of ER-Ca 2+ store and clusters in ER-PM junctions where it associates with and gates Ca 2+ influx channels, Orai1 and TRPC1. Clustering of TRPC1 with STIM1 and Orai1 in these junctions is critical since Orai1-mediated Ca 2+ entry triggers surface expression of TRPC1 while STIM1 gates the channel. Thus, plasma membrane function of TRPC1 depends on the delivery of the channel to the sites where STIM1 puncta are formed. This study examines intracellular trafficking mechanism(s) that determine plasma membrane expression and function of TRPC1 in cells where Orai1 and TRPC1 are endogenously expressed and contribute to Ca 2+ entry. We report that TRPC1 is internalized by Arf6-dependent pathway, sorted to Rab5-containing early endosomes, and trafficked to ER-PM junctions by Rab4-dependent fast recycling. Overexpression of Arf6, or Rab5, but not the respective dominant negative mutants, induced retention of TRPC1 in early endosomes and suppressed TRPC1 function. Notably, cells expressing Arf6 or Rab5 displayed an inwardly rectifying I CRAC current that is mediated by Orai1 instead of TRPC1-associated I SOC , demonstrating that Orai1 function was not altered. Importantly, expression of Rab4, but not STIM1, with Rab5 rescued surface expression and function of TRPC1, restoring generation of I SOC. Together, these data demonstrate that trafficking via fast recycling endosomes determines TRPC1-STIM1 clustering within ER-PM junctions following ER-Ca 2+ store depletion which is critical for the surface expression and function of the channel. Ca 2+ influx mediated by TRPC1 modifies Ca 2+-dependent physiological response of cells.

Science Signaling, 2015

STIM2 increases physiological responses to agonist stimulation by promoting clustering of STIM1 a... more STIM2 increases physiological responses to agonist stimulation by promoting clustering of STIM1 and activation of Orai1.

Handbook of Experimental Pharmacology, 2014

The TRP-canonical (TRPC) subfamily, which consists of seven members (TRPC1-TRPC7), are Ca(2+)-per... more The TRP-canonical (TRPC) subfamily, which consists of seven members (TRPC1-TRPC7), are Ca(2+)-permeable cation channels that are activated in response to receptor-mediated PIP2 hydrolysis via store-dependent and store-independent mechanisms. These channels are involved in a variety of physiological functions in different cell types and tissues. Of these, TRPC6 has been linked to a channelopathy resulting in human disease. Two key players of the store-dependent regulatory pathway, STIM1 and Orai1, interact with some TRPC channels to gate and regulate channel activity. The Ca(2+) influx mediated by TRPC channels generates distinct intracellular Ca(2+) signals that regulate downstream signaling events and consequent cell functions. This requires localization of TRPC channels in specific plasma membrane microdomains and precise regulation of channel function which is coordinated by various scaffolding, trafficking, and regulatory proteins.

Cell Calcium, 2014

Highlights Ca 2+-permeable TRPC channels contribute to various cellular functions TRPC channel tr... more Highlights Ca 2+-permeable TRPC channels contribute to various cellular functions TRPC channel trafficking is a key determinant of their plasma membrane function This review discusses the mechanisms and pathways regulating TRPC trafficking The topics discussed are endocytic, recycling, as well as exocytic mechanisms *Highlights (for review) A c c e p t e d M a n u s c r i p t 1 Trafficking mechanisms and regulation of TRPC Channels.

PLoS ONE, 2014

Phospholipase D (PLD) has been implicated in many cellular functions, such as vesicle trafficking... more Phospholipase D (PLD) has been implicated in many cellular functions, such as vesicle trafficking, exocytosis, differentiation, and proliferation. The aim of this study was to characterize the role of PLD in HSY cells, a human cell line originating from the intercalated duct of the parotid gland. As the function and intracellular localization of PLD varies according to cell type, initially, the intracellular localization of PLD1 and PLD2 was determined. By immunofluorescence, PLD1 and PLD2 both showed a punctate cytoplasmic distribution with extensive co-localization with TGN-46. PLD1 was also found in the nucleus, while PLD2 was associated with the plasma membrane. Treatment of cells with the primary alcohol 1-butanol inhibits the hydrolysis of phosphatidylcoline by PLD thereby suppressing phosphatidic acid (PA) production. In untreated HSY cells, there was only a slight co-localization of PLD with the clathrin coated vesicles. When HSY cells were incubated with 1butanol the total number of clathrin coated vesicles increased, especially in the juxtanuclear region and the co-localization of PLD with the clathrin coated vesicles was augmented. Transmission electron microscopy confirmed that the number of Golgi-associated coated vesicles was greater. Treatment with 1-butanol also affected the Golgi apparatus, increasing the volume of the Golgi saccules. The decrease in PA levels after treatment with 1-butanol likewise resulted in an accumulation of enlarged lysosomes in the perinuclear region. Therefore, in HSY cells PLD appears to be involved in the formation of Golgi associated clathrin coated vesicles as well as in the structural maintenance of the Golgi apparatus.

Biochemical Journal, 2014

SOCE (store-operated Ca2+ entry) is mediated via specific plasma membrane channels in response to... more SOCE (store-operated Ca2+ entry) is mediated via specific plasma membrane channels in response to ER (endoplasmic reticulum) Ca2+ store depletion. This route of Ca2+ entry is central to the dynamic interplay between Ca2+ and cAMP signalling in regulating the activity of Ca2+-sensitive adenylate cyclase isoforms (AC1, AC5, AC6 and AC8). Two proteins have been identified as key components of SOCE: STIM1 (stromal interaction molecule 1), which senses ER Ca2+ store content and translocates to the plasma membrane upon store depletion, where it then activates Orai1, the pore-forming component of the CRAC (Ca2+ release-activated Ca2+) channel. Previous studies reported that co-expression of STIM1 and Orai1 in HEK-293 (human embryonic kidney 293) cells enhances Ca2+-stimulated AC8 activity and that AC8 and Orai1 directly interact to enhance this regulation. Nonetheless, the additional involvement of TRPC (transient receptor potential canonical) channels in SOCE has also been proposed. In th...

Biophysical Journal, 2015

Acolhendo a Alfabetização nos Países de Língua Portuguesa, 2010

O conto através do teatro como meio alfabetizador e transmissor das culturas e histórias africana... more O conto através do teatro como meio alfabetizador e transmissor das culturas e histórias africanas. Revista ACOALFAplp:

Revista Brasileira de Ciência Veterinária, 2005

Ateliê Geográfico, 2013

Resumo O objetivo deste artigo é apresentar os resultados teóricos e metodológicos de uma propost... more Resumo O objetivo deste artigo é apresentar os resultados teóricos e metodológicos de uma proposta de trabalho de campo integrado, que envolveram disciplinas da estrutura curricular do curso de Licenciatura em Geografia. O trabalho também é composto por uma reflexão acerca da importância deste meio de ensino na integração teoria e prática e de uma análise integrada, contemplando aspectos sociais e ambientais que se interrelacionam no espaço geográfico. A proposta foi desenvolvida com os alunos do 2° e 3° anos do curso de Geografia da Universidade Estadual de Goiás-Unidade de Minaçu. As disciplinas contempladas foram a Geomorfologia, a Biogeografia, a Geografia Cultural, a Geografia Agrária e a Cartografia Temática, tendo como campo o Parque Nacional Chapada dos Veadeiros e região. Os procedimentos se desenvolveram em duas etapas, uma em sala de aula, onde foram feitas leituras de textos, discussão dos temas a serem desenvolvidos e elaboração dos mapas temáticos, e um segundo momento no campo, onde se procederam à coleta de dados, descrições e análises dos aspectos culturais, agrários e físicos do parque e área do entorno.

Ateliê Geográfico, 2009

Este artigo propõe fazer uma discussão sobre as categorias gênero, raça, espaço e poder na realid... more Este artigo propõe fazer uma discussão sobre as categorias gênero, raça, espaço e poder na realidade das relações sociais. O poder se manifesta tanto de maneira visível quanto invisível dependendo das estratégias do grupo que lhe detém e, em nome do poder político, econômico e cultural. A segregação espacial a que alguns grupos são submetidos é um reflexo da predominância de privilégios de uma classe dominante ao longo da formação social brasileira. A partir de autores/as pesquisadores/as das questões de gênero, feminismo, raça e espaço realizamos uma reflexão sobre o interesse da Geografia Cultural sobre estas temáticas, bem como sobre as relações de poder de grupos dominantes sobre os dominados e a maneira como a herança escravocrata colonial assegurou índices alarmantes de desemprego e subemprego da população negra no mercado de trabalho. Palavras-chave: relações de poder, gênero, espaço, raça Résumé Cet article propose faire une discussion sur les categories genre, race, espace et pouvoir dans les relations sociales. Le pouvoir se manifeste visiblement ou invisiblement, ça dépend des stratégies des groupes, avec le nom du pouvoir politique, econonomique et culturel. La segregation spatial qui quelques groupes souffrent est un reflet de la Ateliê Geográfico Goiânia-GO v. 3, n. 1 abr/2009 p.97-110 página 98

Ciência Animal Brasileira, 2010

Fiscal federal agropecuário do Ministério da Agricultura, Pecuária e Abastecimento 8. Professor d... more Fiscal federal agropecuário do Ministério da Agricultura, Pecuária e Abastecimento 8. Professor de Doenças Infecciosas DVP/FV/UFPel 9. Professor associado da Universidade Federal de Pelotas (UFPel). RESUMO Considerando a importância do VLFe na clínica felina, assim como a possível disseminação do agente a partir de um felino portador sintomático ou assintomático, o estudo tem como objetivo verificar a frequência de viremia pelo VLFe em felinos residentes em Pelotas e Rio Grande, municípios situados na região sul do Brasil. Para isso foi coletado sangue de 120 animais semi-domiciliados para a detecção do retrovírus através da técnica de imunofluorescência indireta (IFI). Detectou-se a viremia em 38,3% (46/120) dos animais estudados, representando uma frequência maior em relação a outros estudos realizados no Brasil, o que confirma a importância deste agente na região estudada.

Journal of Virological Methods, 2009

Meningoencephalitis caused by Bovine herpesvirus type 5 (BoHV-5) is responsible for heavy economi... more Meningoencephalitis caused by Bovine herpesvirus type 5 (BoHV-5) is responsible for heavy economic losses in the cattle industry. As in other Alphaherpesviruses, the envelope glycoprotein IV (gD), which mediates penetration into host cells, is one of the major candidate antigens for a recombinant vaccine, since it induces a strong and persistent immune response. The DNA coding for a truncated form of BoHV-5 gD (tgD) has been cloned into the Pichia pastoris expression vector pPICZalphaB to allow protein secretion into the medium. After induction with methanol, a approximately 55kDa protein was obtained. Enzyme deglycosylation with Endo H showed a smaller size band in SDS-PGAE, with approximately 50kDa, suggesting that tgD has N-linked oligosaccharides and that it is not hyperglycosylated. The approximately 55kDa protein was recognized by several polyclonal antibodies, including polyclonal antibody anti-tgD and polyclonal antibodies of different animal species immunized with BoHV-5 and BoHV-1. This is the first report of BoHV-5 gD expression in yeast. It was shown that the recombinant truncated form of BoHV-5 gD has antigenic and immunogenic properties similar to the native BoHV-5 gD. Expression of tgD as a secreted protein allows simple and inexpensive purification methods that can be used for further studies to evaluate its immunogenicity in cattle.

Journal of Histochemistry & Cytochemistry, 2012

The current study examined the role of PLD2 in the maintenance of mast cell structure. Phospholip... more The current study examined the role of PLD2 in the maintenance of mast cell structure. Phospholipase D (PLD) catalyzes hydrolysis of phosphatidylcholine to produce choline and phosphatidic acid (PA). PLD has two isoforms, PLD1 and PLD2, which vary in expression and localization depending on the cell type. The mast cell line RBL-2H3 was transfected to overexpress catalytically active (PLD2CA) and inactive (PLD2CI) forms of PLD2. The results of this study show that PLD2CI cells have a distinct star-shaped morphology, whereas PLD2CA and RBL-2H3 cells are spindle shaped. In PLD2CI cells, the Golgi complex was also disorganized with dilated cisternae, and more Golgi-associated vesicles were present as compared with the PLD2CA and RBL-2H3 cells. Treatment with exogenous PA led to the restoration of the wild-type Golgi complex phenotype in PLD2CI cells. Conversely, treatment of RBL-2H3 and PLD2CA cells with 1% 1-Butanol led to a disruption of the Golgi complex. The distribution of acidic c...

Journal of Biomedical Materials Research Part B: Applied Biomaterials, 2009

This study evaluated the cytotoxic effects of a carbamide peroxide (CP) bleaching gel at differen... more This study evaluated the cytotoxic effects of a carbamide peroxide (CP) bleaching gel at different concentrations on odontoblast-like cells. Immortalized cells of the MDPC-23 cell line (30,000 cells/cm(2)) were incubated for 48 h. The bleaching gel was diluted in DMEM culture medium originating extracts with different CP concentrations. The amount (microg/mL) of hydrogen peroxide (H(2)O(2)) released from each extract was measured by the leukocrystal violet/horseradish peroxidase enzyme assay. Five groups (n = 10) were formed according to the CP concentration in the extracts: G1-DMEM (control); G2-0.0001% CP (0.025 microg/mL H(2)O(2)); G3-0.001% CP (0.43 microg/mL H(2)O(2)); G4-0.01% CP (2.21 microg/mL H(2)O(2)); and G5-0.1% CP (29.74 microg/mL H(2)O(2)). MDPC-23 cells were exposed to the bleaching gel extracts for 60 min and cell metabolism was evaluated by the MTT assay. Data were analyzed statistically by one-way ANOVA and Tukey's test (alpha = 0.05). Cell morphology was examined by scanning electron microscopy. The percentages of viable cells were as follows: G1, 100%; G2, 89.41%; G3, 82.4%; G4, 61.5%; and G5, 23.0%. G2 and G3 did not differ significantly (p > 0.05) from G1. The most severe cytotoxic effects were observed in G3 and G4. In conclusion, even at low concentrations, the CP gel extracts presented cytotoxic effects. This cytotoxicity was dose-dependent, and the 0.1% CP concentration caused the most intense cytopathic effects to the MDPC-23 cells.