MIRTA ANA VALENTICH - Academia.edu (original) (raw)

Papers by MIRTA ANA VALENTICH

Revista De Educacion En Biologia, 2007

Se presenta una modalidad pedagogica innovadora para el dictado de un curso de posgrado sobre “ M... more Se presenta una modalidad pedagogica innovadora para el dictado de un curso de posgrado sobre “ Modelos experimentales para el estudio de la tumorigenesis ” en un centro de la universidad de origen de los dictantes. Los objetivos del curso teorico-practico fueron introducir en el conocimiento del proceso de tumorigenesis y analizar diferentes modelos experimentales para este estudio en diversos organos. El dictado fue distribuido entre una etapa a distancia denominada de “Introduccion” y una presencia o de “Desarrollo”. La primera etapa permitio nivelar, en el grupo heterogeneo de cursantes, los conocimientos sobre tumorigenesis necesarios a fin de pasar a la instancia de desarrollo, la cual arrojo resultados satisfactorios.

Diabetes mellitus tipo 2 y otras patologias relacionadas con un estado proinflamatorio cronico (h... more Diabetes mellitus tipo 2 y otras patologias relacionadas con un estado proinflamatorio cronico (hipertension arterial, aterosclerosis, enfermedad de Alzheimer y cancer ) se producen en ciertos individuos, debido a la acumulacion de alteraciones en el organismo, a traves del tiempo. El proposito de este proyecto es elaborar indices confiables, clinicos y bioquimicos que podrian servir como marcadores de perturbaciones en el organismo que pueden ser indicadores fiables de la manifestacion clinica de diabetes mellitus tipo 2 y si estos parametros son modificables por habitos dietarios saludables y practica de ejercicios fisicos. Estas medidas simples y economicas podrian ser empleadas efectivamente para evitar o posponer la aparicion de diabetes mellitus tipo 2 en sujetos en riesgo.

Prostaglandins, Leukotrienes and Essential Fatty Acids, 2001

A numberof experimental and epidemiological studiesindicate that dietary polyunsaturated fattyaci... more A numberof experimental and epidemiological studiesindicate that dietary polyunsaturated fattyacids (PUFA) playa modulatory role in the development of several cancers. However, literature on the importance of dietary PUFA in urinary-tract tumourigenesis is scarce, and even contradictory.Therefore, our purpose was to evaluate comparatively, several urothelial cellular parameters linked to neoplasia when180 BALB/c mice were initiated with the tumourigenic agent melamine and fed with two amounts of different PUFA.In experiment1, mice were fed with 6% of fish oil (enriched in n-3 PUFA, FO), corn oil (enriched in n-6, CO) and olein (enriched in n-9, an EFA deficiency inducer) formulae plus two chow-fed control lots with (CM) and without (C) melamine treatment.In experiment 2, each of the three varieties of PUFA were offered at10%.Following18^22 weeks of melamine treatment, animals were autopsied. The liver fattyacid profile showed a close correlation with the dietary sources, exhibiting in the O group macroscopic and biochemical EFA-deficient (EFAD) characteristics. The frequency of simple urothelial hyperplasias (H) and dysplasia/carcinoma in situ (D/CIS) was significantly lower in the FO group, whereas both types of lesions increased in the CO and O groups, compared to the C and CM mice. Increased proliferation and abnormal luminal localized mitosis were more frequently recorded in EFAD mice, whereas abnormal apoptotic/mitosis ratio increased in both olein-and corn-oil-fed animals. This study shows that dietary PUFA modulate differentially normal and pre-neoplastic proliferation when induced by the tumorigenic agent melamine. Fish oil, rich in n-3 fatty acids, exhibits a clear antipromoting activity, whereas the role of n-6 and n-9 PUFA derivatives needs further research.

Journal of Food Lipids, 2009

ABSTRACT Salivary tumors were induced by 9,10-dimethyl-1,2-benzanthracene injection into the subm... more ABSTRACT Salivary tumors were induced by 9,10-dimethyl-1,2-benzanthracene injection into the submandibulary gland of mice fed with different dietary lipids. Tumor tissue from 10 mice fed with diets containing corn oil (CO) or fish oil (FO) were immunohistochemically stained for Cx26, β- and -catenin, BAX, Bcl-2 and Ki-67. The percentage of animals showing different levels of biomarker expression in epithelial, mesenchymal and stromal cells was calculated in each group. The number of nuclei positive for Ki-67 per 500 total cells was calculated.Reduced expression of Cx26 was observed in FO group as compared to CO. The staining was similar for β-catenin, -catenin, BAX and Bcl-2 in both groups. The Ki-67 labeling index was significantly different for CO and FO (0.45–0.40, P < 0.0008).The lower expression of Ki-67 in FO mice could indicate less cellular proliferation.PRACTICAL APPLICATIONSDietary fatty acids (FAs) affect tumorigenesis. This study examines the effect of dietary lipids on proliferation, apoptosis, cell adhesion and intercellular communication level change during tumorigenesis. Since dietary lipids may be protective or act as promoters of tumorigenesis, the beneficial and negative effects of different FAs could help inform nutrition recommendations directed to the population.

Journal of experimental therapeutics & oncology, 2011

Lung cancer is one of the most important avoidable causes of death around the world, the most wid... more Lung cancer is one of the most important avoidable causes of death around the world, the most widespread carcinoma, with a very poor prognosis, and is the leading cause of cancer death in both developed and developing countries. We report morphological and biological behavior characteristics of a tumor that arose in only one BALB/c mouse of an experimental group treated with urethane, a chemical lung-tumorigenic agent. Morphological and immunochemical analysis indicated phenotypic compatibility with a lung adenocarcinoma. The tumor was named LAC1 (lung adenocarcinoma 1). Implant success in eight LAC1-bearing mice generations was 100%, with a fast evolution (58 survival days) and good metastatic capacity (41% of animals with metastases). The tumor induced a paraneoplastic syndrome characterized by anemia, neutrophilia, cachexia, splenomegaly and thymic atrophy. The lymphoproliferation to Con A was altered in tumor-bearing mice. This lung adenocarcinoma may be a useful experimental mo...

International Journal of Pancreatology, 1991

The present report is a study of the effect of the carcinogen nitrosomethylurea (NMU) on pancreas... more The present report is a study of the effect of the carcinogen nitrosomethylurea (NMU) on pancreas of rats receiving during lifetime a lipid-poor diet, that is essential fatty acid deficient or control diets. Rats fed a commercial stock chow were mated. At day 10 of pregnancy, dams were divided into three groups, that were respectively supplied with the commercial chow, the essential fatty acid deficient or the sufficient diet. Each litter was separated at random in two groups that received at day one of life one intraperitoneal injection of NMU (50 mg/kg b.w.) or saline. After weaning, they were maintained for life with the diet that was supplied to their mothers. The pancreas of NMU-treated rats presented diffuse proliferative changes, focal acinar cell hyperplasias (FACH), and focal hepatocyte-like metaplasia (FHLCM). FACH were expansive presumably preneoplastic growths, showing abnormal differentiation. The number of NMU-treated rats bearing FACH and FHLCM did not significantly differ in the three nutritional conditions.

El campo de accion de un Doctor egresado del Doctorado en Ciencias de la Salud se extiende, hoy, ... more El campo de accion de un Doctor egresado del Doctorado en Ciencias de la Salud se extiende, hoy, mas alla de las perspectivas laborales que venian desarrollando. Inicialmente las perspectivas estaban circunscritas al ambito academico, sea dentro de la Universidad o de organismos gubernamentales promotores de ciencia y tecnologia, tales como el Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET). Otra posicion mas tradicionalista tomaba la obtencion de este titulo como cierre consagratorio de la vida profesional. Actualmente, en consonancia con tendencias internacionales y nacionales, es posible considerar otros campos de accion de un Doctor en Ciencias de la Salud, con un amplio abanico de posibilidades laborales y un nuevo perfil destinado a cubrirlas, enfrentando las exigencias de este siglo XXI...

Journal of Leukocyte Biology, 1986

Revista de la Facultad de Ciencias Medicas (Cordoba, Argentina), Dec 26, 2018

Plant extracts can be obtained to carry bioactive compounds, useful for prevention and treatment ... more Plant extracts can be obtained to carry bioactive compounds, useful for prevention and treatment of different illnesses. This also supports the intake of teas as functional beverages. Nonetheless, it is incompletely known whether these extracts can act as effective sources and vehicles de phenolic compounds (phenolics/polyphenols) to reach their targets. To establish whether phytoextract ingestion modified in a sex-dependent manner the phenolic bioavailability and redox response in liver and kidney. BALB/C mice ingested for a month 100 mg/Kg/d of extracts (tea-like) from Aspidosperma quebracho-blanco (AQB), Lantana grisebachii (LG) or Ilex paraguariensis (IP). Then, phenolics, peroxides and nitrites were analyzed by spectrophotometry. Also, phenolic permeation from digested and undigested extracts was evaluated in vitro with a rat jejunum-based assay. Phenolic permeation depended on extract digestion. In males, IP showed a special time course of hepatic phenolics, whereas all extrac...

Nutrition and Cancer, 2017

ABSTRACT Lung cancer is the leading cause of death by cancer, and is a major sanitary concern wor... more ABSTRACT Lung cancer is the leading cause of death by cancer, and is a major sanitary concern worldwide. Some nutrients, such as ω-9 fatty acids, have been proposed as anticancer agents. Thus, an olein-enriched diet was assayed in a murine model of lung adenocarcinoma (LAC-1) to evaluate neoplastic and paraneoplastic evolution in BALB/c mice. The organic assimilation of dietary fatty acids was confirmed in liver by gas chromatography. This experimental oleic acid-containing diet increased animal survival and tumour latency (analysed by the Kaplan-Meier method), improving neoplastic evolution and general status, with weak effects on the paraneoplastic syndrome (thymus atrophy, splenomegaly, splenocyte response to mitogen, blood anaemia, and leucocytosis). Tumour lipid oxidation was not involved. Thus, diet enrichment with olein, a natural source of the ω-9 oleic acid, significantly delayed progression of LAC-1 and increased tumour latency and mice survival. These results support its use in nutritional management of cancer, with further studies being encouraged.

Existiria asociacion en el desarrollo de tumores salivales, mamarios y prostaticos que deberia al... more Existiria asociacion en el desarrollo de tumores salivales, mamarios y prostaticos que deberia alertar en el seguimiento de esos pacientes. Por ello, resulta indispensable la busqueda de biomarcadores especificos a fin de diagnosticar precozmente un segundo tumor primario o una lesion metastasica.La relacion dieta-cancer es aun controvertida y poco estudiada en humanos. La influencia de ciertos compuestos dietarios sobre la tumorigenesis de diversos organos lleva a plantear que el estado nutricional de un individuo puede ser un indicador de riesgo de desarrollar dicha enfermedad.Se trabajara con sujetos de ambos sexos que presenten diagnostico reciente de tumores salivales, mamarios y prostaticos, sin tratamiento previo y proveniente de los hospitales Privado y Cordoba. El grupo control estara constituido por sujetos con caracteristicas similares a las de los casos, aunque sin diagnostico de tumores. El numero previsto es de 20 casos por cada tipo de tumor -entre benignos y malignos- y 80 controles (relacion 1:2, respectivamente) por ano de trabajo.Para recolectar los datos se empleara una encuesta de frecuenvia alimentaria cuali-cuantitativa, ademas de una historia clinica. En muestras de saliva y sangre se analizaran biomarcadores tumorales como CEA, CA 15-3 y PSA y se identificaran y cuantificaran lipidos totales y acidos grasos. En tejido tumoral se cuantificaran lipidos y marcadores tumorales.

European journal of nutrition, Jun 28, 2016

European journal of nutrition, Jan 18, 2015

The aim of this study was to determine whether α-linolenic acid (ALA ω-3 fatty acid) enriched die... more The aim of this study was to determine whether α-linolenic acid (ALA ω-3 fatty acid) enriched diet affects growth parameters when applied to a syngeneic model of mammary carcinoma. BALB/c mice were divided and fed with: 1) a chia oil diet, rich in ALA or 2) a corn oil diet, rich in linoleic acid (LA ω-6 fatty acid). Mice were subcutaneously inoculated with a tumor cell line LM3, derived from a murine mammary adenocarcinoma. After 35 days, tumor incidence, weight, volume and metastasis number were lower in the ALA-fed mice, while tumor latency time was higher, and the release of pro-tumor metabolites derived from ω-6 fatty acids decreased in the tumor. Compared to the control group, a lower number of mitosis, a higher number of apoptotic bodies and higher T-lymphocyte infiltration were consistently observed in the ALA group. An ALA-rich diet decreased the estrogen receptor (ER) α expression, a recognized breast cancer promotor while showing an opposite effect on ERβ in tumor lysates....

Boletin Latinoamericano y del Caribe de Plantas Medicinales y Aromaticas

Journal of the California Dental Association, 2011

Salivary lipids have been scarcely studied, and the reported results present disparities. This li... more Salivary lipids have been scarcely studied, and the reported results present disparities. This literature review is presented based on the importance of saliva as a diagnostic and/or prognostic medium for various diseases, its lipid content, and on its potential use for the analysis of nutritional markers that contribute to the study of diseases related to lipid consumption and metabolism.

Revista de la Facultad de Ciencias Médicas (Córdoba, Argentina), 2007

The purpose of this study was to analyze the influence of diets varying in lipids and proteins on... more The purpose of this study was to analyze the influence of diets varying in lipids and proteins on the histopathologic variety of murine salivary tumors induced by DMBA. 117 BALB/c mice were assigned to experiments one (E1: lipids, males) and two (E2: proteins, males and females), E1 comprising Soy oil (SO); Corn oil (CO, control); Fish oil (FO) and Olein (O) groups and E2, soy protein (SP) and casein (C) groups. Tumors were induced by DMBA and the animals were sacrificed at week 13- post-induction. Tumor volume was calculated. Tumor sections were stained with H-E for histopathologic evaluation. No significant association was found between tumor volume and dietary condition (p > 0.05). In E1, FO animals developed mainly carcinomas (C) (58.8%), the sarcomas (S) and carcinosarcomas (CS) being especially of high-grade type (tumors < 600 mm3). In E2, SP animals developed mainly C (55.6%). Although no significantly different (p > 0.05), S and C were more frequent in female and ma...

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Revista de la Facultad de Ciencias Médicas (Córdoba, Argentina), 2004

According to the concept of field defects during the carcinogenesis process, excessive epithelial... more According to the concept of field defects during the carcinogenesis process, excessive epithelial proliferation/apoptosis may exist in areas near tumors. Proliferation or apoptosis could be modified by dietary lipids. The present study was designed to analyze proliferation and apoptosis in tongue epithelium of mice fed diets based on different lipids followed by induction of salivary tumors with DMBA. Forty-five days after weaning, ten BALB/c mice were assigned to two diets: corn oil (CO) and fish oil (cod liver, FO). Two weeks later, DMBA was injected in the submandibular area. Animals were sacrificed at the 13th post-injection week. Samples of tongue were fixed in formalin-ethanol and immunohistochemically stained for proliferation (Ki-67) and apoptosis (Bax). By light microscopy, the number of nuclei positive for these markers were counted out of three-hundred total interphase cells both in dorsal and in ventral tongue surfaces. Results were analyzed through Analysis of Variance ...

Journal of experimental therapeutics & oncology, 2010

Since curcumin, a polyphenol extracted from the rhizomes of Curcuma longa L. (Zingiberaceae), has... more Since curcumin, a polyphenol extracted from the rhizomes of Curcuma longa L. (Zingiberaceae), has been proposed for breast cancer chemoprevention, the aim of the present work was to determine if it had anti-tumour effects on mammary cells which are resistant to oxidative damage. ZR-75-1 cells were treated with curcumin and copper(II) sulphate in order to evaluate cell death and gamma-glutamyltranspeptidase (GGTP) activity. Curcumin was cytotoxic in a dose-dependent manner (loss of viability with lactate-dehydrogenase release) with apoptotic effects on ZR-75-1 cells. Also, curcumin displayed an antioxidant effect only on the copper-oxidized cells. The GGTP activity was decreased in a dose-dependent manner by curcumin, with the changes in this parameter accounting for neoplastic inhibition (direct relation between the enzyme activity and cellular viability). Summing up, our results suggest that curcumin induced apoptosis in ZR-75-1 with an antioxidant activity performed on those treat...

Current Nutrition & Food Science, 2009

Carcinogenesis involves a growing accumulation of genetic and epigenetic aberrations, leading to ... more Carcinogenesis involves a growing accumulation of genetic and epigenetic aberrations, leading to the deregulation of cellular homeostasis, followed by neoplastic progression. Although nutritional lipids play a critical role the specific transcriptional mechanisms involved in this process are not completely understood. In this review, we examine the biological effects of dietary essential fatty acids (n-3 and n-6 EFAs) and vitamin A, and the common pathways related to cancer chemoprevention. Eicosanoids (EFAs derivates) and retinoids (vitamin A derivates) are major mediators that act on their corresponding RXR-heterodimerized receptors (PPAR and RAR) and modify the carcinogenetic signalling pathways. Several effects of these mediators, mainly at DNA level, depend on specific molecular properties of the receptor isoforms and their differential affinities for their ligands, whose availability can be intentionally managed through diet. Nevertheless, the previous grade of differentiation in normal development or in cancer cells is an important modulatory factor of the cellular responses, especially when differentiating agents are evaluated. The potential of dietary EFAs and retinoids in chemoprevention and chemotherapy, through their actions on the cellular proliferation and differentiation processes, with particular reference to human breast cancer is discussed herein.

Prostaglandins, Leukotrienes and Essential Fatty Acids, 2005