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Papers by Mahantesh Nagmoti

Indian journal of health sciences and biomedical research KLEU, 2022

Journal of SAFOG, 2013

Objectives: Group B Streptococcus (GBS) has been recognized as the leading cause of serious neona... more Objectives: Group B Streptococcus (GBS) has been recognized as the leading cause of serious neonatal infections through mother-fetal vertical transmission in the west, however, in India, its spectrum is largely under estimated. The present study was carried out to find the incidence of rectovaginal carriage of GBS in parturient women, association with risk factors in mother and to study the neonatal outcome. Materials and methods: A one year cross-sectional prospective study was carried out in 905 parturient women admitted at a tertiary care center meeting the selection criteria over a period of one year from June 2007 to May 2008. The inclusion criteria were all pregnant women admitted to the labor room after 35 ± 1 week of gestation. Rectal and vaginal swabs were taken and cultured on selective Todd-Hewitt broth medium followed by sub culture on blood agar and confirmation by Latex agglutination test from all the women meeting the inclusion criteria. The outcomes measured were the incidence, antenatal risk factors in mother and the neonatal outcome. Analysis was done using paired 't' test, chi-square test and a p-value of <0.05 was taken as statistically significant. Results: Incidence of group B Streptococcus (GBS) was 12.15% and detection rate was increased by 4.6% with the inclusion of rectal swabs for culture. GBS carriage was significantly increased with preterm birth (OR 8.3, 95% CI,1.1-15.5), premature rupture of membranes (OR 7.5, 95% CI, 1.1-13.4), prolonged duration of ruptured membranes more than 10 hours (OR 21, 95% CI,15.2-34.2) and intrapartum temperature more than 38°C(OR 3.1, 95% CI, 0.43-6.66). Birth weight less than 2.5 kg and neonatal intensive care admissions were significantly more (35.45%) in infants of GBS positive women. Conclusion: GBS colonization was more frequent in women with risk factors. GBS pick up rate was increased by the inclusion of both rectal and vaginal swabs.

Indian journal of health sciences and biomedical research KLEU, 2022

BJOG: An International Journal of Obstetrics & Gynaecology

Journal of Medical Microbiology, Feb 1, 2007

Human brucellosis, a zoonotic disease, is endemic in the Belgaum district, Karnataka, India. A ma... more Human brucellosis, a zoonotic disease, is endemic in the Belgaum district, Karnataka, India. A male patient presented with a generalized itchy rash. Blood was sent for venereal disease research laboratory testing. Screening was carried out for Brucella antibodies following hospital policy and diagnosis was confirmed by PCR.

Recent Research in Science and Technology, Jan 8, 2011

Background: HIV-2 is less common worldwide as compared to HIV-1. We conducted a retrospective stu... more Background: HIV-2 is less common worldwide as compared to HIV-1. We conducted a retrospective study regarding the presentation of HIV-2 in Belgaum area. Material and Methods: We screened suspected HIV patients using rapid and ELISA techniques. All reactive patients were differentiated in to HIV-1 & 2 by Western blot. HIV-2 infected patients were screened for opportunistic infections depending on clinical presentations. Results: Twelve patients found to be infected only with HIV-2 infection. Chronic diarrhoea and weight loss was the commonest symptom. Four patients had Cryptosporidial infection alone (33.33%), three with Isospora belli alone (25%) and one with both infections (8.33%). Three patient’s sputum samples were positive for AFB (25%) and one among them had oral and oesophageal Candidiasis ( Candida albicans ) (8.33%). One patient CSF sample showed capsulated yeast cells in negative stain and in culture Cryptococcus neoformans was isolated (8.33%). Conclusions: Along with HIV-1, HIV-2 is also sporadically circulating in our area. As compare to HIV-1, HIV-2 also presented with same clinical presentation and same spectrum opportunistic infections.

Recent Research in Science and Technology, Mar 2, 2011

Brucellosis is a zoonotic disease and endemic in Belgaum. It is characterized by myriad of nonspe... more Brucellosis is a zoonotic disease and endemic in Belgaum. It is characterized by myriad of nonspecific symptoms like fever, nocturnal sweating, backache, osteoarticular symptoms along with complications. Clinical presentation of this infection is variable as it may manifest a systemic disease. Herewith we present three cases of blood culture positive B. mellitensis in pediatric patients. Routine screening for Brucellosis by slide agglutination test can be more helpful in diagnosing PUO.

Indian Journal of Medical Microbiology, 2008

Anaerobic gram-negative bacteria (AGNB) produce enzymes that play a signiÞ cant role in the devel... more Anaerobic gram-negative bacteria (AGNB) produce enzymes that play a signiÞ cant role in the development of disease. We tested 50 AGNB isolates, 25 each from clinically diseased and healthy human sites for in vitro production of caseinase, collagenase, etc. Majority of the isolates were Bacteroides fragilis and Porphyromonas gingivalis, which more commonly produced collagenase and haemolysin. Comparatively larger number of clinical AGNB produced collagenase (P = 0.004). No such difference was observed with other enzymes. Hence, collagenase is probably one of the key virulence markers of pathogenic AGNB, and the inhibitors targeting collagenases might help in the therapy of anaerobic infections.

International Journal of Std & Aids, Oct 1, 2012

India has high prevalence of HIV infection. As HIV and HCV share common routes of transmission, t... more India has high prevalence of HIV infection. As HIV and HCV share common routes of transmission, there is every chance of an HIV-infected patient also having HCV infection. We investigated the prevalence of HCV infection in HIV seropositive individuals attending a tertiary care hospital at Belgaum, south India. A total of 16,124 serum samples from suspected patients were screened for the presence of HIV antibodies and those who tested positive for HIV were screened for HCV antibodies by using enzyme-linked immunosorbent assay (ELISA). A total of 24 (3.52%) patients were found to be co-infected with HIV and HCV. Among them, 16 (66.66%) were male and 8 (33.33%) were female. This study has revealed a relatively high prevalence of HIV/ HCV co-infection in and around Belgaum which suggests preventive and control measures should be taken against the spread of such infection in this part of India.

Indian Journal of Medical Microbiology, 2006

One of the complications of brucellosis is infective endocarditis, which carries a high mortality... more One of the complications of brucellosis is infective endocarditis, which carries a high mortality rate if undiagnosed or misdiagnosed. We report a case of Brucella infective endocarditis, which was diagnosed serologically and by polymerase chain reaction. After Brucella specific treatment, patient showed dramatic improvement clinically, as evident by echocardiogram findings and other investigations.

Archives of Medicine and Health Sciences, 2021

Background: 500,000 new cases with progressive cancer of cervix are reported worldwide annually. ... more Background: 500,000 new cases with progressive cancer of cervix are reported worldwide annually. This malignant cancer is the second common cancer among females. Human papillomavirus (HPV) is considered as the major cause of cervical cancer and dysplasia. PCR Application to detect the HPV DNA in clinical specimens besides microscopic examination of cervical biopsy (Papanicolaou smear) (Pap smear) are valuable prognostic indicators in cervical cancer management. Objectives: Due to the absence of epidemiological data on prevalence and distribution of HPV genotypes in Khuzestan province, the authors decided to conduct a research to determine the HPV genotypes in cases with a degree of cervical dysplasia. Materials and Methods: In this study, 60 samples of paraffin-embedded biopsy samples archived in the library of pathology laboratory of Ahvaz Imam Khomeini hospital were selected for further experiments. After DNA extraction of each specimen, the infection of the tissue with HPV was confirmed by PCR. PCR products were sequenced to detect HPV genotypes. Results: Out of 60 cervical biopsy samples, 8 (%13.3) cases were HPV DNA positive. Four (%50) were genotype 16 positive, 2 (%25) were genotype 6 positive, 1(%12.5) sample was detected as the genotype 18 and 1 of the positive cases was genotype 11 of HPV. Conclusions: Our study shows that prevalence of HPV infection in cervical biopsy samples collected from suspected patients in Khouzestan province is slightly higher than other provinces of Iran. Since HPV cervical infection, is an indicator for the host cervical cancer progression, parallel with Pap smear test, PCR detection of HPV DNA in biopsy of suspected cases is recommended.

Annals of Clinical Microbiology and Antimicrobials, Sep 16, 2005

Background: Of the diverse presentation of neurobrucellosis, intra-medullary spinal cord abscess ... more Background: Of the diverse presentation of neurobrucellosis, intra-medullary spinal cord abscess is extremely rare. Only four other cases have been reported so far. We present a case of spinal cord intra-medullary abscess due to Brucella melitensis. Case presentation: A forty-year-old female presented with progressive weakness of both lower limb with urinary incontinence of 6 months duration. She was febrile. Neurological examination revealed flaccid areflexic paraplegia with T 10 below sensory impairment including perianal region. An intramedullary mass was diagnosed on Magnetic Resonance Image (MRI) scan extending from T 12 to L 2. At surgery, a large abscess was encountered at the conus medullaris, from which Brucella melitensis was grown on culture. She was started on streptomycin and doxycycline for 1 month, followed by rifampicin and doxycycline for 1 month. At 2-year follow-up, she had recovered only partially and continued to have impaired bladder function. Conclusion: Neurobrucellosis, if not treated early, can result in severe neurological morbidity and sequale, which may be irreversible. Hence it is important to consider the possibility of neurobrucellosis in endemic region and treat aggressively.

Indian journal of health sciences and biomedical research KLEU, 2021

BACKGROUND: Women are known to be at high risk of human papillomavirus (HPV) infection and its as... more BACKGROUND: Women are known to be at high risk of human papillomavirus (HPV) infection and its associated cervical pathology. However, limited data are available on circulating genotypes, especially among human immunodeficiency virus (HIV)-negative women. Therefore, the present study was designed to gauge the prevalence of HPV genotypes in HIV-negative women. MATERIALS AND METHODS: This cross-sectional study was conducted on a total of 96 HIV-negative women in the age range of 18–45 years. Cervical samples were collected from the transformation zone of the cervix using the deoxyribonucleic acid collection device (cytobrush), which was rinsed in 20 ml of PreservCyt® vial (Hologic, Inc.). HPV genotyping was done with the linear array HPV genotyping test (Roche, Branchburg, NJ, USA) at National Aids Research Institute, Pune. RESULTS: A significant linear increasing trend in proportion of carcinogenic and noncarcinogenic genotypes over grade was observed (P = 0.039 and P = 0.0024, respectively). HPV 59 was reported to be the most common genotype followed by 16, 53, 62, and 72 but without any statistical significance. CONCLUSION: Screening strategies incorporating HPV genotyping and vaccination should be effective in preventing cervical cancer in HIV-negative women.

Indian Journal of Health Sciences and Biomedical Research (KLEU)

Indian Journal of Health Sciences and Biomedical Research (KLEU)

Recent Research in Science and Technology, 2011

Background: HIV-2 is less common worldwide as compared to HIV-1. We conducted a retrospective stu... more Background: HIV-2 is less common worldwide as compared to HIV-1. We conducted a retrospective study regarding the presentation of HIV-2 in Belgaum area. Material and Methods: We screened suspected HIV patients using rapid and ELISA techniques. All reactive patients were differentiated in to HIV-1 & 2 by Western blot. HIV-2 infected patients were screened for opportunistic infections depending on clinical presentations. Results: Twelve patients found to be infected only with HIV-2 infection. Chronic diarrhoea and weight loss was the commonest symptom. Four patients had Cryptosporidial infection alone (33.33%), three with Isospora belli alone (25%) and one with both infections (8.33%). Three patient’s sputum samples were positive for AFB (25%) and one among them had oral and oesophageal Candidiasis ( Candida albicans ) (8.33%). One patient CSF sample showed capsulated yeast cells in negative stain and in culture Cryptococcus neoformans was isolated (8.33%). Conclusions: Along with HIV...

Journal of Experimental Sciences, Feb 26, 2011

Acinetobacter species are gaining importance in the present days. Here we report isolation of 149... more Acinetobacter species are gaining importance in the present days. Here we report isolation of 149 isolates of Acinetobacter species from various clinical samples from the intensive units of our hospital. We used standard microbiological techniques to identify the isolates and antibiotic sensitivity testing was done by using Kirby-Bauer disc diffusion technique. A.baumanii was the commonest organism in our study and was found multi drug resistant. Â

Archives of Gynecology and Obstetrics, 2010

Objective To determine the prevalence of group B streptococci (GBS) in our population, and to ass... more Objective To determine the prevalence of group B streptococci (GBS) in our population, and to assess the association between risk factors and vaginal flora with maternal rectovaginal colonization. Method Samples were obtained from 405 patients between 35 and 37 weeks of gestation. Swabs from the vaginal and perianal regions were cultured in Todd Hewitt and subcultured in blood agar. Colonies suggestive of GBS were submitted to catalase and CAMP test. The vaginal flora was evaluated on Gram stain vaginal smears. Sociodemographic and obstetric data were obtained by designed form. Considering maternal GBS colonization as the response variable, a logistic regression model was fitted by the stepwise method with quantitative and qualitative explanatory variables. Results The prevalence of GBS colonization was 25.4%. The most frequent vaginal flora abnormalities were cytolytic vaginosis (11.3%), followed by bacterial vaginosis (10.9%), candidosis (8.2%) and intermediate vaginal flora II (8.1%). Logistic regression analysis revealed that maternal age, number of sexual intercourse/week, occurrence of previous spontaneous abortion, presence of candidosis and cytolytic vaginosis were associated with streptococcal colonization. Conclusion The prevalence of GBS is high in pregnant women and is associated with sexual intercourse frequency, previous spontaneous abortion and the presence of candidosis or cytolytic vaginosis.

Indian journal of health sciences and biomedical research KLEU, 2022

Journal of SAFOG, 2013

Objectives: Group B Streptococcus (GBS) has been recognized as the leading cause of serious neona... more Objectives: Group B Streptococcus (GBS) has been recognized as the leading cause of serious neonatal infections through mother-fetal vertical transmission in the west, however, in India, its spectrum is largely under estimated. The present study was carried out to find the incidence of rectovaginal carriage of GBS in parturient women, association with risk factors in mother and to study the neonatal outcome. Materials and methods: A one year cross-sectional prospective study was carried out in 905 parturient women admitted at a tertiary care center meeting the selection criteria over a period of one year from June 2007 to May 2008. The inclusion criteria were all pregnant women admitted to the labor room after 35 ± 1 week of gestation. Rectal and vaginal swabs were taken and cultured on selective Todd-Hewitt broth medium followed by sub culture on blood agar and confirmation by Latex agglutination test from all the women meeting the inclusion criteria. The outcomes measured were the incidence, antenatal risk factors in mother and the neonatal outcome. Analysis was done using paired 't' test, chi-square test and a p-value of <0.05 was taken as statistically significant. Results: Incidence of group B Streptococcus (GBS) was 12.15% and detection rate was increased by 4.6% with the inclusion of rectal swabs for culture. GBS carriage was significantly increased with preterm birth (OR 8.3, 95% CI,1.1-15.5), premature rupture of membranes (OR 7.5, 95% CI, 1.1-13.4), prolonged duration of ruptured membranes more than 10 hours (OR 21, 95% CI,15.2-34.2) and intrapartum temperature more than 38°C(OR 3.1, 95% CI, 0.43-6.66). Birth weight less than 2.5 kg and neonatal intensive care admissions were significantly more (35.45%) in infants of GBS positive women. Conclusion: GBS colonization was more frequent in women with risk factors. GBS pick up rate was increased by the inclusion of both rectal and vaginal swabs.

Indian journal of health sciences and biomedical research KLEU, 2022

BJOG: An International Journal of Obstetrics & Gynaecology

Journal of Medical Microbiology, Feb 1, 2007

Human brucellosis, a zoonotic disease, is endemic in the Belgaum district, Karnataka, India. A ma... more Human brucellosis, a zoonotic disease, is endemic in the Belgaum district, Karnataka, India. A male patient presented with a generalized itchy rash. Blood was sent for venereal disease research laboratory testing. Screening was carried out for Brucella antibodies following hospital policy and diagnosis was confirmed by PCR.

Recent Research in Science and Technology, Jan 8, 2011

Background: HIV-2 is less common worldwide as compared to HIV-1. We conducted a retrospective stu... more Background: HIV-2 is less common worldwide as compared to HIV-1. We conducted a retrospective study regarding the presentation of HIV-2 in Belgaum area. Material and Methods: We screened suspected HIV patients using rapid and ELISA techniques. All reactive patients were differentiated in to HIV-1 & 2 by Western blot. HIV-2 infected patients were screened for opportunistic infections depending on clinical presentations. Results: Twelve patients found to be infected only with HIV-2 infection. Chronic diarrhoea and weight loss was the commonest symptom. Four patients had Cryptosporidial infection alone (33.33%), three with Isospora belli alone (25%) and one with both infections (8.33%). Three patient’s sputum samples were positive for AFB (25%) and one among them had oral and oesophageal Candidiasis ( Candida albicans ) (8.33%). One patient CSF sample showed capsulated yeast cells in negative stain and in culture Cryptococcus neoformans was isolated (8.33%). Conclusions: Along with HIV-1, HIV-2 is also sporadically circulating in our area. As compare to HIV-1, HIV-2 also presented with same clinical presentation and same spectrum opportunistic infections.

Recent Research in Science and Technology, Mar 2, 2011

Brucellosis is a zoonotic disease and endemic in Belgaum. It is characterized by myriad of nonspe... more Brucellosis is a zoonotic disease and endemic in Belgaum. It is characterized by myriad of nonspecific symptoms like fever, nocturnal sweating, backache, osteoarticular symptoms along with complications. Clinical presentation of this infection is variable as it may manifest a systemic disease. Herewith we present three cases of blood culture positive B. mellitensis in pediatric patients. Routine screening for Brucellosis by slide agglutination test can be more helpful in diagnosing PUO.

Indian Journal of Medical Microbiology, 2008

Anaerobic gram-negative bacteria (AGNB) produce enzymes that play a signiÞ cant role in the devel... more Anaerobic gram-negative bacteria (AGNB) produce enzymes that play a signiÞ cant role in the development of disease. We tested 50 AGNB isolates, 25 each from clinically diseased and healthy human sites for in vitro production of caseinase, collagenase, etc. Majority of the isolates were Bacteroides fragilis and Porphyromonas gingivalis, which more commonly produced collagenase and haemolysin. Comparatively larger number of clinical AGNB produced collagenase (P = 0.004). No such difference was observed with other enzymes. Hence, collagenase is probably one of the key virulence markers of pathogenic AGNB, and the inhibitors targeting collagenases might help in the therapy of anaerobic infections.

International Journal of Std & Aids, Oct 1, 2012

India has high prevalence of HIV infection. As HIV and HCV share common routes of transmission, t... more India has high prevalence of HIV infection. As HIV and HCV share common routes of transmission, there is every chance of an HIV-infected patient also having HCV infection. We investigated the prevalence of HCV infection in HIV seropositive individuals attending a tertiary care hospital at Belgaum, south India. A total of 16,124 serum samples from suspected patients were screened for the presence of HIV antibodies and those who tested positive for HIV were screened for HCV antibodies by using enzyme-linked immunosorbent assay (ELISA). A total of 24 (3.52%) patients were found to be co-infected with HIV and HCV. Among them, 16 (66.66%) were male and 8 (33.33%) were female. This study has revealed a relatively high prevalence of HIV/ HCV co-infection in and around Belgaum which suggests preventive and control measures should be taken against the spread of such infection in this part of India.

Indian Journal of Medical Microbiology, 2006

One of the complications of brucellosis is infective endocarditis, which carries a high mortality... more One of the complications of brucellosis is infective endocarditis, which carries a high mortality rate if undiagnosed or misdiagnosed. We report a case of Brucella infective endocarditis, which was diagnosed serologically and by polymerase chain reaction. After Brucella specific treatment, patient showed dramatic improvement clinically, as evident by echocardiogram findings and other investigations.

Archives of Medicine and Health Sciences, 2021

Background: 500,000 new cases with progressive cancer of cervix are reported worldwide annually. ... more Background: 500,000 new cases with progressive cancer of cervix are reported worldwide annually. This malignant cancer is the second common cancer among females. Human papillomavirus (HPV) is considered as the major cause of cervical cancer and dysplasia. PCR Application to detect the HPV DNA in clinical specimens besides microscopic examination of cervical biopsy (Papanicolaou smear) (Pap smear) are valuable prognostic indicators in cervical cancer management. Objectives: Due to the absence of epidemiological data on prevalence and distribution of HPV genotypes in Khuzestan province, the authors decided to conduct a research to determine the HPV genotypes in cases with a degree of cervical dysplasia. Materials and Methods: In this study, 60 samples of paraffin-embedded biopsy samples archived in the library of pathology laboratory of Ahvaz Imam Khomeini hospital were selected for further experiments. After DNA extraction of each specimen, the infection of the tissue with HPV was confirmed by PCR. PCR products were sequenced to detect HPV genotypes. Results: Out of 60 cervical biopsy samples, 8 (%13.3) cases were HPV DNA positive. Four (%50) were genotype 16 positive, 2 (%25) were genotype 6 positive, 1(%12.5) sample was detected as the genotype 18 and 1 of the positive cases was genotype 11 of HPV. Conclusions: Our study shows that prevalence of HPV infection in cervical biopsy samples collected from suspected patients in Khouzestan province is slightly higher than other provinces of Iran. Since HPV cervical infection, is an indicator for the host cervical cancer progression, parallel with Pap smear test, PCR detection of HPV DNA in biopsy of suspected cases is recommended.

Annals of Clinical Microbiology and Antimicrobials, Sep 16, 2005

Background: Of the diverse presentation of neurobrucellosis, intra-medullary spinal cord abscess ... more Background: Of the diverse presentation of neurobrucellosis, intra-medullary spinal cord abscess is extremely rare. Only four other cases have been reported so far. We present a case of spinal cord intra-medullary abscess due to Brucella melitensis. Case presentation: A forty-year-old female presented with progressive weakness of both lower limb with urinary incontinence of 6 months duration. She was febrile. Neurological examination revealed flaccid areflexic paraplegia with T 10 below sensory impairment including perianal region. An intramedullary mass was diagnosed on Magnetic Resonance Image (MRI) scan extending from T 12 to L 2. At surgery, a large abscess was encountered at the conus medullaris, from which Brucella melitensis was grown on culture. She was started on streptomycin and doxycycline for 1 month, followed by rifampicin and doxycycline for 1 month. At 2-year follow-up, she had recovered only partially and continued to have impaired bladder function. Conclusion: Neurobrucellosis, if not treated early, can result in severe neurological morbidity and sequale, which may be irreversible. Hence it is important to consider the possibility of neurobrucellosis in endemic region and treat aggressively.

Indian journal of health sciences and biomedical research KLEU, 2021

BACKGROUND: Women are known to be at high risk of human papillomavirus (HPV) infection and its as... more BACKGROUND: Women are known to be at high risk of human papillomavirus (HPV) infection and its associated cervical pathology. However, limited data are available on circulating genotypes, especially among human immunodeficiency virus (HIV)-negative women. Therefore, the present study was designed to gauge the prevalence of HPV genotypes in HIV-negative women. MATERIALS AND METHODS: This cross-sectional study was conducted on a total of 96 HIV-negative women in the age range of 18–45 years. Cervical samples were collected from the transformation zone of the cervix using the deoxyribonucleic acid collection device (cytobrush), which was rinsed in 20 ml of PreservCyt® vial (Hologic, Inc.). HPV genotyping was done with the linear array HPV genotyping test (Roche, Branchburg, NJ, USA) at National Aids Research Institute, Pune. RESULTS: A significant linear increasing trend in proportion of carcinogenic and noncarcinogenic genotypes over grade was observed (P = 0.039 and P = 0.0024, respectively). HPV 59 was reported to be the most common genotype followed by 16, 53, 62, and 72 but without any statistical significance. CONCLUSION: Screening strategies incorporating HPV genotyping and vaccination should be effective in preventing cervical cancer in HIV-negative women.

Indian Journal of Health Sciences and Biomedical Research (KLEU)

Indian Journal of Health Sciences and Biomedical Research (KLEU)

Recent Research in Science and Technology, 2011

Background: HIV-2 is less common worldwide as compared to HIV-1. We conducted a retrospective stu... more Background: HIV-2 is less common worldwide as compared to HIV-1. We conducted a retrospective study regarding the presentation of HIV-2 in Belgaum area. Material and Methods: We screened suspected HIV patients using rapid and ELISA techniques. All reactive patients were differentiated in to HIV-1 & 2 by Western blot. HIV-2 infected patients were screened for opportunistic infections depending on clinical presentations. Results: Twelve patients found to be infected only with HIV-2 infection. Chronic diarrhoea and weight loss was the commonest symptom. Four patients had Cryptosporidial infection alone (33.33%), three with Isospora belli alone (25%) and one with both infections (8.33%). Three patient’s sputum samples were positive for AFB (25%) and one among them had oral and oesophageal Candidiasis ( Candida albicans ) (8.33%). One patient CSF sample showed capsulated yeast cells in negative stain and in culture Cryptococcus neoformans was isolated (8.33%). Conclusions: Along with HIV...

Journal of Experimental Sciences, Feb 26, 2011

Acinetobacter species are gaining importance in the present days. Here we report isolation of 149... more Acinetobacter species are gaining importance in the present days. Here we report isolation of 149 isolates of Acinetobacter species from various clinical samples from the intensive units of our hospital. We used standard microbiological techniques to identify the isolates and antibiotic sensitivity testing was done by using Kirby-Bauer disc diffusion technique. A.baumanii was the commonest organism in our study and was found multi drug resistant. Â

Archives of Gynecology and Obstetrics, 2010

Objective To determine the prevalence of group B streptococci (GBS) in our population, and to ass... more Objective To determine the prevalence of group B streptococci (GBS) in our population, and to assess the association between risk factors and vaginal flora with maternal rectovaginal colonization. Method Samples were obtained from 405 patients between 35 and 37 weeks of gestation. Swabs from the vaginal and perianal regions were cultured in Todd Hewitt and subcultured in blood agar. Colonies suggestive of GBS were submitted to catalase and CAMP test. The vaginal flora was evaluated on Gram stain vaginal smears. Sociodemographic and obstetric data were obtained by designed form. Considering maternal GBS colonization as the response variable, a logistic regression model was fitted by the stepwise method with quantitative and qualitative explanatory variables. Results The prevalence of GBS colonization was 25.4%. The most frequent vaginal flora abnormalities were cytolytic vaginosis (11.3%), followed by bacterial vaginosis (10.9%), candidosis (8.2%) and intermediate vaginal flora II (8.1%). Logistic regression analysis revealed that maternal age, number of sexual intercourse/week, occurrence of previous spontaneous abortion, presence of candidosis and cytolytic vaginosis were associated with streptococcal colonization. Conclusion The prevalence of GBS is high in pregnant women and is associated with sexual intercourse frequency, previous spontaneous abortion and the presence of candidosis or cytolytic vaginosis.