PCR confirmation of cutaneous manifestation due to Brucella melitensis (original) (raw)
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Clinical profile of brucellosis from a tertiary care center in southern India
Asian Pacific journal of tropical medicine, 2011
To highlight the spectrum of clinical manifestations, labs, complications, treatment and outcome of brucellosis. Retrospective study was conducted in Kasturba Medical College, Manipal University, Karnataka, India which included 68 confirmed cases of brucellosis from January 2006- April 2010. Diagnosis of brucellosis was made by culturing the sera/body fluids by standard BACTEC method (or) by testing the sera for Brucella agglutinins using the standard agglutination test (SAT). A titer of 1:320 or more was considered as significant. SPSS 16 was used for statistical analysis and Microsoft Excel for graphical representation. Of the 68 patients, 46 (68%) were male and 22 (32%) were female patients with age distribution of 9-75 years. Forty four (64.7%) had history of contact with unpasteurized dairy products or infected animals. Symptoms included fever (68, 100%), myalgia (21, 31%), musculoskeletal symptoms (23, 34%), headache (16, 24%), gastrointestinal symptoms (19, 28%) and altered s...
Human Brucellosis : Still an Unfamiliar and Misdiagnosed Disease in India
2015
Background: Human brucellosis is a disease with protean clinical manifestations. Despite many awareness programmes, it is still missed or wrongly diagnosed. This leads to chronic morbidity leading to misery and loss of working days. Aim and Objectives: To assess the microbiological, clinical and epidemiological aspects of human brucellosis. Materials and Methods: Patients with positive brucella screening test constituted the study material. A detailed laboratory, clinical, epidemiological study along with response to the treatment was analyzed. Results: Seroprevalence of brucellosis was found to be 1.75%. Brucellosis was clinically diagnosed in only 12.73% of cases. Fever, joint pain and low backache were the commonest symptoms. Close contact with animals and raw milk ingestion were the major sources of infection. Knowledge regarding brucellosis and its prevention was lacking in patients. Brucellosis was not considered as one of the differential diagnosis by the treating physicians....
Seroepidemiology of Human Brucellosis in Karnataka
A seroprevalence study of brucellosis in humans was undertaken among high risk population of north Karnataka. A total of 201 serum samples were collected from various high risk group individuals and subjected to three different tests i.e., Rose Bengal plate test (RBPT), standard tube agglutination test (STAT) and 2 mercaptoethanol test (2-MET). On the analysis of 201 serum samples, 30 (14.92%) were positive to RBPT, 33 (16.41%) to STAT and 8 (3.98%) to 2-MET. The prevalence of brucellosis was found to be highest among individuals in the age group of >50 yrs and lowest in age group of <20 yrs. Sex wise prevalence of brucellosis was more prevalent among males compared to females.
Indian Journal of Medical Research, 2016
Background & objectives: Brucellosis is endemic in the southern part of India. A combination of biochemical, serological and molecular methods is required for identification and biotyping of Brucella. The present study describes the isolation and biochemical, molecular characterization of Brucella melitensis from patients suspected for human brucellosis. Methods: The blood samples were collected from febrile patients suspected to have brucellosis. A total of 18 isolates were obtained from 102 blood samples subjected to culture. The characterization of these 18 isolates was done by growth on Brucella specific medium, biochemical reactions, CO 2 requirement, H 2 S production, agglutination with A and M mono-specific antiserum, dye sensitivity to basic fuchsin and thionin. Further, molecular characterization of the isolates was done by amplification of B. melitensis species specific IS711 repetitive DNA fragment and 16S (rRNA) sequence analysis. PCR-restriction fragment length polymorphism (RFLP) analysis of omp2 locus and IS711 gene was also done for molecular characterization. Results: All 102 suspected samples were subjected to bacteria isolation and of these, 18 isolates could be recovered on blood culture. The biochemical, PCR and PCR-RFLP and 16s rRNA sequencing revealed that all isolates were of B. melitensis and matched exactly with reference strain B. melitensis 16M. Interpretation & conclusions: The present study showed an overall isolation rate of 17.64 per cent for B. melitensis. There is a need to establish facilities for isolation and characterization of Brucella species for effective clinical management of the disease among patients as well as surveillance and control of infection in domestic animals. Further studies are needed from different geographical areas of the country with different level of endemicity to plan and execute control strategies against human brucellosis.
Childhood Brucellosis in Eastern India
Indian journal of pediatrics, 2017
To investigate the presence of childhood brucellosis presenting as PUO (pyrexia of unknown origin) cases in Eastern zone of India. Blood samples were collected from PUO patients aged ≤18 y. The main diagnostic tools were STAT, RBPT, ELISA- IgM, IgG and PCR. Although mainly PUO cases were selected for the study, other associated clinical manifestations were also noted. The findings revealed significantly higher percentage of infection in female children (14.3%) than in male children (10.9%). The positive results by different diagnostic tools, STAT, RBPT, ELISA- IgM, ELISA-IgG and brucella genus specific PCR were 10.6%, 7.2%, 7.2%, 0.85% and 1.3% respectively. Main associated clinical symptoms were joint pain, low backache, fatigue and night sweat. This hospital based study reflects a significant number of childhood brucellosis cases in Eastern zone of India, and thus emphasizes the need for further monitoring of such subjects.
Serological evidence of Brucellosis in selected gaushalas of Haryana
The Indian Journal of Animal Sciences
Brucellosis is a neglected zoonotic disease with significant economic and public health consequences to human and animal population in developing countries. The objective of the present study was to determine the serological evidences of brucellosis in cattle reared in two gaushalas of Hisar and Jind districts, Haryana. The serological tests: Rose Bengal Plate Test (RBPT), Serum Agglutination Test (SAT) and Enzyme Linked Immunosorbent Assay (ELISA) were employed for screening the animals for brucellosis. The overall seropositivity by RBPT, SAT and ELISA was 23.46%, 20.67% and 28.49% respectively. The logistic regression modalities concluded higher likelihood of brucellosis with age > 6 years followed by 3–6 year than cows with <3 years. The agreement between tests (RBPT and ELISA, SAT and ELISA and RBPT and SAT) was found to be 0.87 (95% CI: 0.857–0.882), 0.70 (95% CI: 0.684–0.718) and 0.82 (95% CI: 0.809–0.834) respectively by kappa statistic. This study concluded high infect...
Human brucellosis in a non-susceptible host: a case report
International Journal of Advances in Medicine
Clinical human brucellosis is quite rare in India, that too in an non susceptible host. This report describes a case of clinical human brucellosis in India. This case involved a 36 years old male, a professor in an Engineering college. He presented with complaints of fever for 4 months with myalgia, generalized tiredness, loss of weight and loss of appetite. On evaluation, total counts and ESR was elevated and all other investigations were normal. Based on history, common causes like occult tuberculosis or autoimmune diseases were considered. But, blood cultures grew Brucella melitensis in all 2 samples and a diagnosis of brucellosis was made. He was treated with Rifampicin and Doxycycline. Suspicion of brucellosis in this patient is low because the patient has no history of contact with animals, consumption of unpasteurized milk or occupational exposure. So, the health care practitioners should be aware of this possibility of this zoonotic infection as a differential diagnosis in p...
2020
Brucellosis is an important zoonotic disease and has public health importance. In the present study, we studied the prevalence and associated risk factors of human brucellosis in the central Indian population from tertiary care health settings. A prospective observational study was conducted from March 2015 to February 2018 in patients attending the outpatient department (OPD) of Central India Institute of Medical Sciences (CIIMS), Nagpur. A total of 7026 individuals suspected of brucellosis were recruited based on prespecified inclusion criteria, additional risk factors, and clinical symptoms. Baseline, demographic and clinical characteristics were likewise recorded. Sera samples from recruited individuals were collected and subjected to anti-brucellosis antibody (IgM) detection using a commercial kit by ELISA assay. The overall seroprevalence of brucellosis reported from tertiary care health settings was 11% (772/7026). The majority of positive cases were from the states Madhya Pr...
Brucella melitensis review of the human infection case
Bosnian Journal of …, 2006
During the last several years, brucellosis has become an important public-health problem on a large territory part of Bosnia and Herzegovina. The disease belongs to the zoonosis group, and can be caused by several bacterium species from Brucella genus. For human and veterinarian medicine, B. abortus, B. melitensis, B. suis and B. canis from Brucella genus are important, while other brucella species are found only in animals. The results of laboratory process of isolating Brucella melitensis, as well as of detection of specific antibacterial antibodies, are presented in this work. Namely, B. melitensis was isolated from blood samples (chemo-culture), as a causal agent of disease in one sixty years-old patient, treated during . In pair serum samples of the patient, the presence of specific anti-brucella antibodies was confirmed qualitatively and quantitatively. In the serum I, ELISA test confirmed the presence of specific IgM antibodies of , U/ml, and IgG antibodies of U/ml. In the serum II, IgM antibodies of , U/ml, and IgG antibodies of U/ml were found. These results suggest and confirm established work diagnosis, and etiology causality of the disease with isolated bacterium.