Mahmoud Aalkader - Academia.edu (original) (raw)

Mahmoud Aalkader

Professor of Biophysics, Suez Canal University, Ismailia, Egypt.Interested in studying the molecular mechanisms during biological abnormalities (diseases).I have many publications about Cataract, Leukemia, Renal failure, Diabetes, ..

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Papers by Mahmoud Aalkader

Research paper thumbnail of Oxidative stress as a common mediator for apoptosis induced-cardiac damage in diabetic rats

The Open …, 2008

Aim:To investigate the possible role of oxidative stress as a common mediator of apoptosis and ca... more Aim:To investigate the possible role of oxidative stress as a common mediator of apoptosis and cardiac damage in diabetes.Materials and Methods:This experimental work was conducted on 5 groups of Wistar rats. Group I was the control group. Diabetes type 1 was induced in other groups (by streptozotocin) and animals received insulin or vitamin E (300 mg /kg body weight), both insulin and vitamin E, or no treatment for 4 weeks according to their group. At the end of the study, serum and cardiac tissues were examined for biochemical parameters of cardiac function, oxidative stress and apoptosis. Electron microscopy pictures of cardiac tissue were also evaluated for signs of cardiac damageResults: Markers of oxidative stress, apoptosis, inflammation as well as manifestations of cardiac damage as assessed by electron microscopy were significantly decreased in rats treated with both insulin and vitamin E when compared with untreated diabetic rats or rats treated with either insulin or vita...

Research paper thumbnail of THE INFLUENCE OF 50 Hz MAGNETIC FIELD ON LIVER FUNCTION

The present study has been conducted to evaluate the influence of different intensities of 50 Hz ... more The present study has been conducted to evaluate the influence of different intensities of 50 Hz magnetic field on some liver function tests such as alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), bilirubin, albumin and protein.

Research paper thumbnail of SPECTROSCOPIC STUDY ON OXIDATIVE REACTIONS OF NORMAL AND PATHOGENIC HEMOGLOBIN MOLECULES

ABSTRACT Hemoglobin (Hb) of normal erythrocytes undergoes oxidative reactions with constant rate.... more ABSTRACT Hemoglobin (Hb) of normal erythrocytes undergoes oxidative reactions with constant rate. In anemia, hemoglobin is exposed to oxidative denaturation. Oxidation of oxy- hemoglobin gives O-2 and met-hemoglobin (met-Hb). If the globin structure of hemoglobin molecule is destabilized, met-hemoglobin can be converted to hemichrome. Reaction of hemoglobin with radical or oxidant-generating system and structure denaturation can be followed by spectral analysis. In this study, different oxidation products of hemoglobin were measured in three types of anemia; severe iron deficiency, glucose-6-phosphate dehydrogenase deficiency and β-thalassemia. The results showed that hemoglobin suffers from an increase in the auto-oxidation rate in case of anemia followed by an enhancement in both met-hemoglobin and hemichrome levels and a decrease in the oxy-hemoglobin concentration.

Research paper thumbnail of Oxidative stress as a common mediator for apoptosis induced-cardiac damage in diabetic rats

The Open …, 2008

Aim:To investigate the possible role of oxidative stress as a common mediator of apoptosis and ca... more Aim:To investigate the possible role of oxidative stress as a common mediator of apoptosis and cardiac damage in diabetes.Materials and Methods:This experimental work was conducted on 5 groups of Wistar rats. Group I was the control group. Diabetes type 1 was induced in other groups (by streptozotocin) and animals received insulin or vitamin E (300 mg /kg body weight), both insulin and vitamin E, or no treatment for 4 weeks according to their group. At the end of the study, serum and cardiac tissues were examined for biochemical parameters of cardiac function, oxidative stress and apoptosis. Electron microscopy pictures of cardiac tissue were also evaluated for signs of cardiac damageResults: Markers of oxidative stress, apoptosis, inflammation as well as manifestations of cardiac damage as assessed by electron microscopy were significantly decreased in rats treated with both insulin and vitamin E when compared with untreated diabetic rats or rats treated with either insulin or vita...

Research paper thumbnail of THE INFLUENCE OF 50 Hz MAGNETIC FIELD ON LIVER FUNCTION

The present study has been conducted to evaluate the influence of different intensities of 50 Hz ... more The present study has been conducted to evaluate the influence of different intensities of 50 Hz magnetic field on some liver function tests such as alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), bilirubin, albumin and protein.

Research paper thumbnail of SPECTROSCOPIC STUDY ON OXIDATIVE REACTIONS OF NORMAL AND PATHOGENIC HEMOGLOBIN MOLECULES

ABSTRACT Hemoglobin (Hb) of normal erythrocytes undergoes oxidative reactions with constant rate.... more ABSTRACT Hemoglobin (Hb) of normal erythrocytes undergoes oxidative reactions with constant rate. In anemia, hemoglobin is exposed to oxidative denaturation. Oxidation of oxy- hemoglobin gives O-2 and met-hemoglobin (met-Hb). If the globin structure of hemoglobin molecule is destabilized, met-hemoglobin can be converted to hemichrome. Reaction of hemoglobin with radical or oxidant-generating system and structure denaturation can be followed by spectral analysis. In this study, different oxidation products of hemoglobin were measured in three types of anemia; severe iron deficiency, glucose-6-phosphate dehydrogenase deficiency and β-thalassemia. The results showed that hemoglobin suffers from an increase in the auto-oxidation rate in case of anemia followed by an enhancement in both met-hemoglobin and hemichrome levels and a decrease in the oxy-hemoglobin concentration.

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