Marc Kreuger - Academia.edu (original) (raw)

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AMAR DHERE

University Of Pune'S AFFILIATED INDIRA COLLEGE OF COMMERCE AND SCIENCE, PUNE

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Papers by Marc Kreuger

Two monoclonal antibodies (ZUM 15 and ZUM 18) directed against carrot (Daucus carota L.) seed ara... more Two monoclonal antibodies (ZUM 15 and ZUM 18) directed against carrot (Daucus carota L.) seed arabinogalactan proteins (AGPs) were used to isolate specific AGP fractions. For both carrot and tomato (Lyco persicon esculentum Mill.) seed AGPs analyzed by crossed electrophoresis, the ZUM 15 and ZUM 18 AGP fractions showed one identical peak. However, the Rf values for the two species were different: 0.82 for carrot seed AGPs and 0.52 for tomato seed AGPs. When the fractionated AGPs (carrot or tomato) were added to carrot cell lines they had a dramatic effect on the culture. One AGP fraction (ZUM 15 AGPs) was able to induce vacuolation of embryogenic cells. Those cells failed to produce embryos. The other AGP fraction (ZUM 18 AGPs) increased the percentage of embryogenic cells from about 40% up to 80% within one week and this subsequently resulted in the formation of more embryos on hormone-free medium. This activity was higher than that of unfractionated carrot seed AGPs, while the opt...

For indoor farming to become the next step in agriculture, it is essential that most nutritious a... more For indoor farming to become the next step in agriculture, it is essential that most nutritious and healthy crops can be grown indoors. Some fruits and vegetables currently grown in (high-tech) greenhouses should be suitable from an economic point of view; cost price is relatively high as are the produced volumes. One example for such a crop is the high-wire cultivated varieties of tomatoes. Breeding companies can take the advantage of this technology. Breeding for taste and nutrition only and not having to focus on (a)biotic stress is now possible. This chapter describes the growth and production of a tomato crop in an indoor farm. Growth, development, and fruit yield at least equal greenhouse performance showing the opportunities ahead for these types of crops.

Cell & developmental biology, 2000

Indoor farming can contribute to the world food production in the coming decades. Indoor growing ... more Indoor farming can contribute to the world food production in the coming decades. Indoor growing would greatly reduce the need for water and pesticides to grow crops and enable the production of safe, clean, nutritious, and affordable food. However, to realize the yields needed, some of the plant’s requirements must be met. For this a climate system was designed where light, temperature, and evaporation are controlled independently. In the modular system, a laminar airflow controls evaporation, independent of light levels and without impact of infrared light. Together with crop models, calculations and predictions of yields can be made, which are necessary to define commercial success in advance.

The large scale propagation of plants via somatic embryogenesis, has so far been difficult to ach... more The large scale propagation of plants via somatic embryogenesis, has so far been difficult to achieve. In this thesis research is described leading to embryogenic cell lines that can be maintained for a long period, without loss of genetic stability. It is also described how embryogenic potential of cell lines can be influenced by the addition of specific arabinogalactan-proteins.We consider

Cell and Developmental Biology of Arabinogalactan-Proteins, 2000

Physiologia Plantarum, 1996

Raaijmakers, N. and van Hoist, G.-J. 1996. Genetically stable celi lines of cucumber for the larg... more Raaijmakers, N. and van Hoist, G.-J. 1996. Genetically stable celi lines of cucumber for the large-scale production of diploid somatic embryos.-Physiol. Plant. 97: 303-310. For the initiation of embr\'ogenic cucumber (Cucumis sativus L.) cell lines, from excised radicles, directly in liquid medium, the culture regime, explant density' and type and concentration of hormones were adjusted so that pro-embryogenic masses (PEMs) were formed within about 8 weeks. The established cucumber cell lines were maintained for severai years without loss of embryogenic and genetic stability. The ploidy level of somatic embryos from different cucumber eel! lines was either diploid or tetraploid and depended on the ploidy level of Ihe cell line. Cucumber cell lines that produced only diploid embryos were obtained by selecting completely diploid explant material and growing 11 in the dark during the initiation phase, Mixoploid explants could lead to tetraploid or mixoploid cell lines. Isolation and additional selection and subculturing of single PEMs resulted in either completely diploid or tetraploid eel! lines, indicating that all ceils of individual PEMs are either diploid or tetraploid. The embryogenic cucumber cell lines, differing only in ploidy level, were indistinguishable in growth rate and embrj'ogenic potential and were genetically stable over several years.

Two monoclonal antibodies (ZUM 15 and ZUM 18) directed against carrot (Daucus carota L.) seed ara... more Two monoclonal antibodies (ZUM 15 and ZUM 18) directed against carrot (Daucus carota L.) seed arabinogalactan proteins (AGPs) were used to isolate specific AGP fractions. For both carrot and tomato (Lyco persicon esculentum Mill.) seed AGPs analyzed by crossed electrophoresis, the ZUM 15 and ZUM 18 AGP fractions showed one identical peak. However, the Rf values for the two species were different: 0.82 for carrot seed AGPs and 0.52 for tomato seed AGPs. When the fractionated AGPs (carrot or tomato) were added to carrot cell lines they had a dramatic effect on the culture. One AGP fraction (ZUM 15 AGPs) was able to induce vacuolation of embryogenic cells. Those cells failed to produce embryos. The other AGP fraction (ZUM 18 AGPs) increased the percentage of embryogenic cells from about 40% up to 80% within one week and this subsequently resulted in the formation of more embryos on hormone-free medium. This activity was higher than that of unfractionated carrot seed AGPs, while the opt...

For indoor farming to become the next step in agriculture, it is essential that most nutritious a... more For indoor farming to become the next step in agriculture, it is essential that most nutritious and healthy crops can be grown indoors. Some fruits and vegetables currently grown in (high-tech) greenhouses should be suitable from an economic point of view; cost price is relatively high as are the produced volumes. One example for such a crop is the high-wire cultivated varieties of tomatoes. Breeding companies can take the advantage of this technology. Breeding for taste and nutrition only and not having to focus on (a)biotic stress is now possible. This chapter describes the growth and production of a tomato crop in an indoor farm. Growth, development, and fruit yield at least equal greenhouse performance showing the opportunities ahead for these types of crops.

Cell & developmental biology, 2000

Indoor farming can contribute to the world food production in the coming decades. Indoor growing ... more Indoor farming can contribute to the world food production in the coming decades. Indoor growing would greatly reduce the need for water and pesticides to grow crops and enable the production of safe, clean, nutritious, and affordable food. However, to realize the yields needed, some of the plant’s requirements must be met. For this a climate system was designed where light, temperature, and evaporation are controlled independently. In the modular system, a laminar airflow controls evaporation, independent of light levels and without impact of infrared light. Together with crop models, calculations and predictions of yields can be made, which are necessary to define commercial success in advance.

The large scale propagation of plants via somatic embryogenesis, has so far been difficult to ach... more The large scale propagation of plants via somatic embryogenesis, has so far been difficult to achieve. In this thesis research is described leading to embryogenic cell lines that can be maintained for a long period, without loss of genetic stability. It is also described how embryogenic potential of cell lines can be influenced by the addition of specific arabinogalactan-proteins.We consider

Cell and Developmental Biology of Arabinogalactan-Proteins, 2000

Physiologia Plantarum, 1996

Raaijmakers, N. and van Hoist, G.-J. 1996. Genetically stable celi lines of cucumber for the larg... more Raaijmakers, N. and van Hoist, G.-J. 1996. Genetically stable celi lines of cucumber for the large-scale production of diploid somatic embryos.-Physiol. Plant. 97: 303-310. For the initiation of embr\'ogenic cucumber (Cucumis sativus L.) cell lines, from excised radicles, directly in liquid medium, the culture regime, explant density' and type and concentration of hormones were adjusted so that pro-embryogenic masses (PEMs) were formed within about 8 weeks. The established cucumber cell lines were maintained for severai years without loss of embryogenic and genetic stability. The ploidy level of somatic embryos from different cucumber eel! lines was either diploid or tetraploid and depended on the ploidy level of Ihe cell line. Cucumber cell lines that produced only diploid embryos were obtained by selecting completely diploid explant material and growing 11 in the dark during the initiation phase, Mixoploid explants could lead to tetraploid or mixoploid cell lines. Isolation and additional selection and subculturing of single PEMs resulted in either completely diploid or tetraploid eel! lines, indicating that all ceils of individual PEMs are either diploid or tetraploid. The embryogenic cucumber cell lines, differing only in ploidy level, were indistinguishable in growth rate and embrj'ogenic potential and were genetically stable over several years.

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