Marek Luczynski - Academia.edu (original) (raw)
Papers by Marek Luczynski
Environmental biotechnology, 2007
Gynogenetic northern pike (Esox lucius L.) were produced using UV irradiated sperm and heat shock... more Gynogenetic northern pike (Esox lucius L.) were produced using UV irradiated sperm and heat shock applied to inseminated eggs shortly after gamete activation. Milt was diluted in immobilizing solution (1:9) and UV irradiated (6.4 W·m-2) for 2-20 min, with dosage in the range of 768-7680 J·m-2. Genetic inactivation of spermatozoa was most efficient when milt was irradiated for 8 min (3072 J·m-2). Insemination of eggs with irradiated milt yielded 100% haploid larvae with hatching rate at 72.1±0.8% (mean±SD), expressed as a percentage of inseminated eggs. Haploid embryo developed and most of them hatched (showing ”haploid syndrome”) but all haploid larvae died within 48 hours after hatching. After insemination with irradiated sperm the eggs were exposed to a thermal shock of 34°C or 34.5°C, lasting 3 or 5 min, applied 11-16 min after gamete activation. The efficiency of heat shock and survival in experimental groups significantly depended on the source (individual female effect) and qu...
International Journal of Environmental Research and Public Health, 2017
Mercury content and fatty acids in muscles of Perca fluviatilis L. (European perch), Leuciscus id... more Mercury content and fatty acids in muscles of Perca fluviatilis L. (European perch), Leuciscus idus L. (ide), Cyprinus carpio L. (European or common carp), Oncorhynchus mykiss Walb. (rainbow trout), Platichthys flesus L. (European flounder). and Clupea harengus L. (bream) from the Polish market were investigated. The total mercury was processed with AAS. The fatty acids were analyzed by gas chromatography. The concentration of mercury in muscles varied from 0.006 to 0.138 mg/kg and decreased as follows: perch ≈ ide > flounder > herring ≈ bream ≈ rainbow trout > carp (p ≤ 0.05). There were only significant positive correlations between body weight and mercury content in muscle tissue of carp (r = 0.878), flounder (r = 0.925) and herring (r = 0.982) (p ≤ 0.05). The atherogenic index (AI), thrombogenicity index (TI) and flesh-lipid quality index (FLQ) were calculated as follows 0.33-0.70 (IA), 0.16-0.31 (IT) and 13.01-33.22 (FLQ). Hypocholesterolemic (OFA) and hypercholesterolemic fatty acids (DFA) in muscles of fish ranged from 18.26 to 23.01 and from 73.91 to 78.46, respectively. In most cases, there were not significant correlations between size (body weight and total length) and fatty acids in the muscles of the examined fish (p > 0.05). The Target Hazard Quotient (THQ) values were below 1, which shows that there is no non-carcinogenic health risk to the consumer by consuming the examined fish.
Animal Reproduction Science, 2016
The development of a new protocol for egg fertilization may increase embryo survival and benefit ... more The development of a new protocol for egg fertilization may increase embryo survival and benefit the aquaculture process. In the present study, a new technique of partially adding sperm to activated eggs in the artificial fertilization of burbot (Lota lota), ide (Leuciscus idus) and asp (Aspius aspius) eggs was evaluated. If the same volume of sperm was divided into two or three parts and added to eggs in 30-60s intervals, it significantly improved embryo survival at the eyed-egg-stage of development. In the present study, the periodic addition of spermatozoa to eggs affected fertilization (ide and asp) and embryo survival rates (ide, asp and burbot) and might be successfully applied under hatchery conditions.
Archives of Polish Fisheries, 2009
We induced homozygous gynogenesis in Northern pike (Esox lucius L.). The eggs obtained from two f... more We induced homozygous gynogenesis in Northern pike (Esox lucius L.). The eggs obtained from two females and inseminated with UV inactivated milt were subjected to a pressure shock. The shock (600 atm, 3 min duration) was applied 60, 75, 90, 105, 120 or 135 min after activation of gametes. Application of the pressure shock resulted in interruption of the first mitotic division and gynogenetic larvae were obtained. The best results (5.20 0.7% of survival, expressed as average SD) were observed when the eggs from the first female (A) were subjected to a pressure shock 75 min after insemination. Shock applied 90 min after activation resulted in 3.51 2.4% of gynogenetic individuals. Pressure treatments applied 105, 120 or 135 min were ineffective. The results demonstrate that pressure shock can be applied to induce homozygous development in Northern pike. 1
Cytobios, 1996
Cold shock (2 degrees C) lasting for 45 or 60 min was applied to eggs from bream (Abramis brama),... more Cold shock (2 degrees C) lasting for 45 or 60 min was applied to eggs from bream (Abramis brama), beginning at 2, 3, 4, 5, 6, 7 and 8 min after fertilization. The lowest survival rate was observed in those groups treated in which a cold shock started at 4 or 5 min after fertilization. Groups exposed to cold shock lasting for 60 min showed a higher percentage of triploids than in groups shocked for 45 min. The highest yield of triploid embryos was produced by the cold shock which started 8 min after egg fertilization and which lasted for 45 min.
Polish Journal of Natural Science, 2010
A study on reproduction of neon tetra has been carried out under controlled conditions. Neon tetr... more A study on reproduction of neon tetra has been carried out under controlled conditions. Neon tetra is very popular aquarium fish. It was observed that spawners of this species produce viable gametes during a few (5-6) spawning periods only. From the breeding perspective fish of that species should be reproduced again shortly after the completed spawning and time between spawns should be 15 to 20 days. Keeping the fish between spawning periods more than 20 days results in a significant deterioration of quality of gametes, expressed by the decreased number of 12-day-old larvae. It was shown that before spawning spawners should be kept in water at 22 o C. The negative effect of keeping the reproducers in water at 25 o C accumulated with time.
Archives of Polish Fisheries, 2014
The fatty acid compositions were studied in eight commercially important fish from fish markets: ... more The fatty acid compositions were studied in eight commercially important fish from fish markets: salmon, Salmo salar L.; cod, Gadus morhua L.; common sole, Solea solea (L.); European flounder, Platichthys flesus (L.); catfish, Clarias gariepinus (Burchell); rainbow trout, Oncorhynchus mykiss (Walb.); Nile tilapia, Oreochromis niloticus (L.); and pangasius, Pangasianodon hypophthalmus (Sauvage). The freshwater fish contained 25.69-42.18% saturated, 34.90-43.79% monounsaturated, 8.46-16.32% n-6 polyunsaturated, and 5.01-20.43% n-3 polyunsaturated acid, while marine fish contained 18.53-32.77% saturated, 17.95-49.89% monounsaturated, 3.40-11.51% n-6 polyunsaturated, and 18.74-45.42% n-3 polyunsaturated acid. Marine fish contained significantly more Ʃn-3 PUFA (29.79%), EPA (12.26%), DHA (13.20%), and a higher n-3/n-6 (6.95) ratio than freshwater fish (13.13, 2.47, 7.14, 1.29%) (P≤ 0.05). There were statistically significant differences in the n-3/n-6 ratio among fish species (cod (13.40...
Arch Pol Fish, 2001
Spermiation and ovulation in the Eurasian perch Perca fluviatilis L. were induced with Ovopel, pe... more Spermiation and ovulation in the Eurasian perch Perca fluviatilis L. were induced with Ovopel, pellets containing GnRH, and then compared with hCG and CPE. Males from the control group produced a significantly smaller volume of milt (average about 17 ml kg BW-1) than those from the hormonally-treated groups (over 24 ml kg BW-1). Spermatozoa motility was similar in both the treated and untreated males, but the fertilization ability varied significantly between individual males. Over 90% of the treated females ovulated. Ovulation in the control group was noted in a limited number of females. Mean embryo survival to the eyed-egg-stage was over 50% in the treated groups, in contrast to 7.5% in the control group. The poor quality of perch gametes might result from high water temperature oscillations before the natural spawning season. The quality of eggs, expressed as survival to the eyed-egg-stage, decreased with latency time.
Archives of Polish Fisheries, 2008
Lipid content and fatty acids composition of non-predatory fish: roach, Rutilus rutilus (L.), bre... more Lipid content and fatty acids composition of non-predatory fish: roach, Rutilus rutilus (L.), bream, Abramis brama (L.), vendace, Coregonus albula (L.), and of predatory fish: Eurasian perch, Perca fluviatilis L., pike, Esox lucius L. and burbot, Lota lota (L.) were examined. These fish were caught from three lakes of Mazurian Great Lakes (Kisajno, Dargin, Niegocin). The content of total lipid and some fatty acids varied widely within and among species. Generally, the lipid content was low (0.56-2.78%). Among the saturated and monounsaturated fatty acids, the predominant fatty acids were palmitic C16:0 (19.24-33.44%), stearic C18:0 (4.37-6.87%), palmitoleic C16:1 (4.51-12.93%), and oleic C18:1 n-9 (6.85-14.49%). Arachidonic C20:4 n-6 (3.17-6.55%), eicosapentaenoic C20:5 n-3 (4.14-8.91%), and docosahexaenoic C22:6 n-3 (5.91-24.67%) acids were the most abundant polyunsaturated fatty acids. In the case of all the freshwater fish, with the exception of bream, higher contents of saturated fatty acids than monounsaturated fatty acids were noted. Among the fish studied, the highest value of total n-3 polyunsaturated fatty acids (43.86%) was noted in vendace, whereas bream contained the highest content of total n-6 polyunsaturated fatty acids (11.21%). The ratio of n-3/n-6 ranged between 1.50 (burbot) and 4.40 (vendace). Differences in the content of fatty acids in fish with different feeding strategies (non-predatory and predatory) were measured. Non-predatory fish were found to have lower values of saturated fatty acids than predatory fish (P £ 0.05). Non-predatory fish contained significantly more eicosapentaenoic acid (EPA) than predatory fish (P £ 0.01), whereas the amounts of monounsaturated and n-3 and n-6 polyunsaturated fatty acids in non-predatory and predatory fish were not statistically significant (P > 0.05). The total n-3 polyunsaturated fatty acids was higher in non-predatory fish, but not statistically significant (P > 0.05). Similarly, the differences in DHA and n-3/n-6 ratio in muscles of predatory and non-predatory fish were not statistically significant (P > 0.05).
Aquaculture Research, 2012
Aquaculture Research, 1997
Induced spawnitig in breatn, Abramis brama (L.), was studied using acetone-dried common carp pitu... more Induced spawnitig in breatn, Abramis brama (L.), was studied using acetone-dried common carp pituitary (CP) and breatn pituitary (BP) with or without the addition of human chorionic gonadotrophin (hCG). The total dose administered to fish was of 5.0 mg kg"^ BP or 4.0 mg kg"' CP with or without 2000-2200 fU hCG kg-' for females and 2.5 mg kg'' BP or 2.0 mg kg'^ CP with or without 1000-1100 IU HCG kg-' for males. In all male treated groups 100% of spermiation was observed: in females the most effective method was a triple injection with hCG and carp pituitary, resulting in 79% of females ovulated (over 68% of eyed eggs). Biological quality of eggs, expressed as a percentage of eyed eggs, was negatively correlated with time elapsing between resolving (final) injection and ovulation. Spawning success, expressed as a value of Se (spawning effectiveness coefficient), was higher in fish treated with triple Injection.
the control of maturation and spawning on many freshwater fish species belonging to different fam... more the control of maturation and spawning on many freshwater fish species belonging to different families (cyprinids, percids, esocids, coregonids) and offered, within the framework of Luciopercimprove, to prepare a manual on artificial reproduction of pikeperch. This manual has been revised by the different partners of the project in order to produce a practical tool which can be used by all aquaculturists interested to artificially reproduce pikeperch in captivity. The editors and the technical assistant are very grateful to the members of the Luciopercimprove consortium
Environmental biotechnology, 2007
Gynogenetic northern pike (Esox lucius L.) were produced using UV irradiated sperm and heat shock... more Gynogenetic northern pike (Esox lucius L.) were produced using UV irradiated sperm and heat shock applied to inseminated eggs shortly after gamete activation. Milt was diluted in immobilizing solution (1:9) and UV irradiated (6.4 W·m-2) for 2-20 min, with dosage in the range of 768-7680 J·m-2. Genetic inactivation of spermatozoa was most efficient when milt was irradiated for 8 min (3072 J·m-2). Insemination of eggs with irradiated milt yielded 100% haploid larvae with hatching rate at 72.1±0.8% (mean±SD), expressed as a percentage of inseminated eggs. Haploid embryo developed and most of them hatched (showing ”haploid syndrome”) but all haploid larvae died within 48 hours after hatching. After insemination with irradiated sperm the eggs were exposed to a thermal shock of 34°C or 34.5°C, lasting 3 or 5 min, applied 11-16 min after gamete activation. The efficiency of heat shock and survival in experimental groups significantly depended on the source (individual female effect) and qu...
International Journal of Environmental Research and Public Health, 2017
Mercury content and fatty acids in muscles of Perca fluviatilis L. (European perch), Leuciscus id... more Mercury content and fatty acids in muscles of Perca fluviatilis L. (European perch), Leuciscus idus L. (ide), Cyprinus carpio L. (European or common carp), Oncorhynchus mykiss Walb. (rainbow trout), Platichthys flesus L. (European flounder). and Clupea harengus L. (bream) from the Polish market were investigated. The total mercury was processed with AAS. The fatty acids were analyzed by gas chromatography. The concentration of mercury in muscles varied from 0.006 to 0.138 mg/kg and decreased as follows: perch ≈ ide > flounder > herring ≈ bream ≈ rainbow trout > carp (p ≤ 0.05). There were only significant positive correlations between body weight and mercury content in muscle tissue of carp (r = 0.878), flounder (r = 0.925) and herring (r = 0.982) (p ≤ 0.05). The atherogenic index (AI), thrombogenicity index (TI) and flesh-lipid quality index (FLQ) were calculated as follows 0.33-0.70 (IA), 0.16-0.31 (IT) and 13.01-33.22 (FLQ). Hypocholesterolemic (OFA) and hypercholesterolemic fatty acids (DFA) in muscles of fish ranged from 18.26 to 23.01 and from 73.91 to 78.46, respectively. In most cases, there were not significant correlations between size (body weight and total length) and fatty acids in the muscles of the examined fish (p > 0.05). The Target Hazard Quotient (THQ) values were below 1, which shows that there is no non-carcinogenic health risk to the consumer by consuming the examined fish.
Animal Reproduction Science, 2016
The development of a new protocol for egg fertilization may increase embryo survival and benefit ... more The development of a new protocol for egg fertilization may increase embryo survival and benefit the aquaculture process. In the present study, a new technique of partially adding sperm to activated eggs in the artificial fertilization of burbot (Lota lota), ide (Leuciscus idus) and asp (Aspius aspius) eggs was evaluated. If the same volume of sperm was divided into two or three parts and added to eggs in 30-60s intervals, it significantly improved embryo survival at the eyed-egg-stage of development. In the present study, the periodic addition of spermatozoa to eggs affected fertilization (ide and asp) and embryo survival rates (ide, asp and burbot) and might be successfully applied under hatchery conditions.
Archives of Polish Fisheries, 2009
We induced homozygous gynogenesis in Northern pike (Esox lucius L.). The eggs obtained from two f... more We induced homozygous gynogenesis in Northern pike (Esox lucius L.). The eggs obtained from two females and inseminated with UV inactivated milt were subjected to a pressure shock. The shock (600 atm, 3 min duration) was applied 60, 75, 90, 105, 120 or 135 min after activation of gametes. Application of the pressure shock resulted in interruption of the first mitotic division and gynogenetic larvae were obtained. The best results (5.20 0.7% of survival, expressed as average SD) were observed when the eggs from the first female (A) were subjected to a pressure shock 75 min after insemination. Shock applied 90 min after activation resulted in 3.51 2.4% of gynogenetic individuals. Pressure treatments applied 105, 120 or 135 min were ineffective. The results demonstrate that pressure shock can be applied to induce homozygous development in Northern pike. 1
Cytobios, 1996
Cold shock (2 degrees C) lasting for 45 or 60 min was applied to eggs from bream (Abramis brama),... more Cold shock (2 degrees C) lasting for 45 or 60 min was applied to eggs from bream (Abramis brama), beginning at 2, 3, 4, 5, 6, 7 and 8 min after fertilization. The lowest survival rate was observed in those groups treated in which a cold shock started at 4 or 5 min after fertilization. Groups exposed to cold shock lasting for 60 min showed a higher percentage of triploids than in groups shocked for 45 min. The highest yield of triploid embryos was produced by the cold shock which started 8 min after egg fertilization and which lasted for 45 min.
Polish Journal of Natural Science, 2010
A study on reproduction of neon tetra has been carried out under controlled conditions. Neon tetr... more A study on reproduction of neon tetra has been carried out under controlled conditions. Neon tetra is very popular aquarium fish. It was observed that spawners of this species produce viable gametes during a few (5-6) spawning periods only. From the breeding perspective fish of that species should be reproduced again shortly after the completed spawning and time between spawns should be 15 to 20 days. Keeping the fish between spawning periods more than 20 days results in a significant deterioration of quality of gametes, expressed by the decreased number of 12-day-old larvae. It was shown that before spawning spawners should be kept in water at 22 o C. The negative effect of keeping the reproducers in water at 25 o C accumulated with time.
Archives of Polish Fisheries, 2014
The fatty acid compositions were studied in eight commercially important fish from fish markets: ... more The fatty acid compositions were studied in eight commercially important fish from fish markets: salmon, Salmo salar L.; cod, Gadus morhua L.; common sole, Solea solea (L.); European flounder, Platichthys flesus (L.); catfish, Clarias gariepinus (Burchell); rainbow trout, Oncorhynchus mykiss (Walb.); Nile tilapia, Oreochromis niloticus (L.); and pangasius, Pangasianodon hypophthalmus (Sauvage). The freshwater fish contained 25.69-42.18% saturated, 34.90-43.79% monounsaturated, 8.46-16.32% n-6 polyunsaturated, and 5.01-20.43% n-3 polyunsaturated acid, while marine fish contained 18.53-32.77% saturated, 17.95-49.89% monounsaturated, 3.40-11.51% n-6 polyunsaturated, and 18.74-45.42% n-3 polyunsaturated acid. Marine fish contained significantly more Ʃn-3 PUFA (29.79%), EPA (12.26%), DHA (13.20%), and a higher n-3/n-6 (6.95) ratio than freshwater fish (13.13, 2.47, 7.14, 1.29%) (P≤ 0.05). There were statistically significant differences in the n-3/n-6 ratio among fish species (cod (13.40...
Arch Pol Fish, 2001
Spermiation and ovulation in the Eurasian perch Perca fluviatilis L. were induced with Ovopel, pe... more Spermiation and ovulation in the Eurasian perch Perca fluviatilis L. were induced with Ovopel, pellets containing GnRH, and then compared with hCG and CPE. Males from the control group produced a significantly smaller volume of milt (average about 17 ml kg BW-1) than those from the hormonally-treated groups (over 24 ml kg BW-1). Spermatozoa motility was similar in both the treated and untreated males, but the fertilization ability varied significantly between individual males. Over 90% of the treated females ovulated. Ovulation in the control group was noted in a limited number of females. Mean embryo survival to the eyed-egg-stage was over 50% in the treated groups, in contrast to 7.5% in the control group. The poor quality of perch gametes might result from high water temperature oscillations before the natural spawning season. The quality of eggs, expressed as survival to the eyed-egg-stage, decreased with latency time.
Archives of Polish Fisheries, 2008
Lipid content and fatty acids composition of non-predatory fish: roach, Rutilus rutilus (L.), bre... more Lipid content and fatty acids composition of non-predatory fish: roach, Rutilus rutilus (L.), bream, Abramis brama (L.), vendace, Coregonus albula (L.), and of predatory fish: Eurasian perch, Perca fluviatilis L., pike, Esox lucius L. and burbot, Lota lota (L.) were examined. These fish were caught from three lakes of Mazurian Great Lakes (Kisajno, Dargin, Niegocin). The content of total lipid and some fatty acids varied widely within and among species. Generally, the lipid content was low (0.56-2.78%). Among the saturated and monounsaturated fatty acids, the predominant fatty acids were palmitic C16:0 (19.24-33.44%), stearic C18:0 (4.37-6.87%), palmitoleic C16:1 (4.51-12.93%), and oleic C18:1 n-9 (6.85-14.49%). Arachidonic C20:4 n-6 (3.17-6.55%), eicosapentaenoic C20:5 n-3 (4.14-8.91%), and docosahexaenoic C22:6 n-3 (5.91-24.67%) acids were the most abundant polyunsaturated fatty acids. In the case of all the freshwater fish, with the exception of bream, higher contents of saturated fatty acids than monounsaturated fatty acids were noted. Among the fish studied, the highest value of total n-3 polyunsaturated fatty acids (43.86%) was noted in vendace, whereas bream contained the highest content of total n-6 polyunsaturated fatty acids (11.21%). The ratio of n-3/n-6 ranged between 1.50 (burbot) and 4.40 (vendace). Differences in the content of fatty acids in fish with different feeding strategies (non-predatory and predatory) were measured. Non-predatory fish were found to have lower values of saturated fatty acids than predatory fish (P £ 0.05). Non-predatory fish contained significantly more eicosapentaenoic acid (EPA) than predatory fish (P £ 0.01), whereas the amounts of monounsaturated and n-3 and n-6 polyunsaturated fatty acids in non-predatory and predatory fish were not statistically significant (P > 0.05). The total n-3 polyunsaturated fatty acids was higher in non-predatory fish, but not statistically significant (P > 0.05). Similarly, the differences in DHA and n-3/n-6 ratio in muscles of predatory and non-predatory fish were not statistically significant (P > 0.05).
Aquaculture Research, 2012
Aquaculture Research, 1997
Induced spawnitig in breatn, Abramis brama (L.), was studied using acetone-dried common carp pitu... more Induced spawnitig in breatn, Abramis brama (L.), was studied using acetone-dried common carp pituitary (CP) and breatn pituitary (BP) with or without the addition of human chorionic gonadotrophin (hCG). The total dose administered to fish was of 5.0 mg kg"^ BP or 4.0 mg kg"' CP with or without 2000-2200 fU hCG kg-' for females and 2.5 mg kg'' BP or 2.0 mg kg'^ CP with or without 1000-1100 IU HCG kg-' for males. In all male treated groups 100% of spermiation was observed: in females the most effective method was a triple injection with hCG and carp pituitary, resulting in 79% of females ovulated (over 68% of eyed eggs). Biological quality of eggs, expressed as a percentage of eyed eggs, was negatively correlated with time elapsing between resolving (final) injection and ovulation. Spawning success, expressed as a value of Se (spawning effectiveness coefficient), was higher in fish treated with triple Injection.
the control of maturation and spawning on many freshwater fish species belonging to different fam... more the control of maturation and spawning on many freshwater fish species belonging to different families (cyprinids, percids, esocids, coregonids) and offered, within the framework of Luciopercimprove, to prepare a manual on artificial reproduction of pikeperch. This manual has been revised by the different partners of the project in order to produce a practical tool which can be used by all aquaculturists interested to artificially reproduce pikeperch in captivity. The editors and the technical assistant are very grateful to the members of the Luciopercimprove consortium