Marissa Noel - Academia.edu (original) (raw)

Papers by Marissa Noel

This work sought to develop a tissue culture method for Vitex negundo L., a popular medicinal her... more This work sought to develop a tissue culture method for Vitex negundo L., a popular medicinal herb with many known biological activities, and for which a number of biologically active metabolites have been identified and isolated from intact plant samples

Vitex negundo L. is a pantropic herb which can be found in South and Southeast Asia. Known locall... more Vitex negundo L. is a pantropic herb which can be found in South and Southeast Asia. Known locally in the Philippines as “lagundi”, V. negundo is a popular medicinal plant that is used as an antitussive, antiasthma and antipyretic (Quisumbing 1978, Pardo de Tavera 2000). A number of compounds have been isolated and identified from the leaves, including sesquiterpenes (β-eudesmol) (Dayrit & Trono 1995), flavonoids (casticin, chrysoplenol D, luteolin and isoorientin) (Dayrit et al.1987), iridoids (2’-para-hydroxybenzoyl mussaenosidic acid, 6’-parahydroxybenzoyl mussaenosidic acid, agnuside and lagundinin) (Dayrit & Lagurin 1994), and minor amounts of the plant steroid β-sitosterol (Joshi et al. 1974). Many other monoterpenes and sesquiterpenes had also been detected in smaller amounts by GC-MS (Mallavarapu et al. 1994). A callus line of Vitex negundo was established using explants from the leaves. The calli showed stable morphology and thin layer chromatographic (TLC) profiles over se...

Philippine Agriculturist, 1980

... Document details. Title The chemical and biochemical composition of legume seeds. 1. Mungbean... more ... Document details. Title The chemical and biochemical composition of legume seeds. 1. Mungbean. Authors Rosario, RR Del; Lozano, Y.; Noel, MG Journal Philippine Agriculturist 1980 Vol. 63 No. 3 pp. 267-274 ISSN 0031-7454 Record Number 19811425851. Abstract. ...

KIMIKA

Glucosinolates in mustard leaves (Brassica juncea L.) and seeds (Brassica nigra L.) were investig... more Glucosinolates in mustard leaves (Brassica juncea L.) and seeds (Brassica nigra L.) were investigated via chemical analysis of their major components and the activity of their hydrolysis products. High-performance liquid chromatography (HPLC) revealed that raw seeds have the highest amount of glucosinolates among the samples tested. Boiling of mustard leaves for 15 minutes significantly reduced the total glucosinolate content (10.94 mmol/g) to up to ~80% in comparison with the raw sample (54.31 mmol/g) which is attributed to thermal degradation of glucosinolates or leaching out to the boiling medium. Similarly, roasting of mustard seeds resulted in a marked decline in total glucosinolate content to 33.70 µmol/g compared with that of raw seed samples (86.18 µmol/g) due to thermal degradation. Liquid chromatography-mass spectrometry (LC-MS) revealed that sinigrin (allyl glucosinolate) is the major glucosinolate in mustard leaves, while the seeds contain sinigrin and gluconapin. Allyl ...

Pharmacognosy Journal

Materials Certification that the mature tubers of several R. sativus varieties used in this study... more Materials Certification that the mature tubers of several R. sativus varieties used in this study were of the same family and species was verified at the National Museum, Botany Division in Manila, Philippines. Extractions and analysis were done immediately to ensure reliability of results. Visual appearance and freedom from defects were considered in the selection of the samples for analyses. Sample preparation In accordance with common culinary practices, white radish tubers were washed and peeled before ABSTRACT This research was conducted to assess the gluocosinolate (GSL), isothiocyanate (ITC) and myrosinase content in two cultivars of Raphanus sativus Linn. (white and red) roots. LC-ESI-MS investigation was done on desulfated GSLs since this methodology has been previously established for efficient GSL analyses. The major GSLs: sinigrin (1) 1,2-dihydroxy-2-phenylethyl glucosinolate (2), 4-hydroxyglucobrassicin (3), glucoraphasatin (4) and 4-methoxyglucobrassicin (5) were found in red radishes; whereas, only 1 and 4 were obtained in white radishes. Myrosinase was analyzed in the tubers due to its ability to catalyze and hydrolyze GSLs into ITCs. This β-thioglucosidase enzyme was found to be over 10 times more active in red tubers (2.05E-02 units) than in white radishes (1.55E-03 units) and the results were linked to the presence/absence of the outer covering of the tubers. Due to the promising medicinal properties of the aglucone derivative of compound 4, 4-methylthio-3-butenyl isothiocyanate (6), the ITC analog was monitored using gas chromatographic mass spectral analyses after myrosinase-mediated hydrolysis. From the results, it can be construed that the occurrence of GSLs 1-5 and the bioactive agent 6 were inherent in the R. sativus cultivars evaluated.

The dichloromethane extracts of the leaves of Brassica oleracea var capitata f. rubra L (red cabb... more The dichloromethane extracts of the leaves of Brassica oleracea var capitata f. rubra L (red cabbage) and Brassica oleracea L (green/white cabbage) and the stem of Brassica oleracea L var. italic (broccoli) afforded β-sitosterol (1) and unsaturated triglycerides (2). The red cabbage also afforded stigmasterol (3), while the green/white cabbage and broccoli stem also yielded the essential fatty acid, linoleic acid (4). Brassica juncea (mustard) leaves and Raphanus sativus (radish) roots afforded 1, and the essential fatty acids 4 and α-linolenic acid (6). Mustard leaves also yielded trilinolenin (5), lutein (7) and β-carotene (8), while radish roots also afforded 2. These compounds were reported to exhibit anticancer properties.

The dichloromethane extracts of the leaves of Brassica oleracea var capitata f. rubra L (red cabb... more The dichloromethane extracts of the leaves of Brassica oleracea var capitata f. rubra L (red cabbage) and Brassica oleracea L (green/white cabbage) and the stem of Brassica oleracea L var. italic (broccoli) afforded β-sitosterol (1) and unsaturated triglycerides (2). The red cabbage also afforded stigmasterol (3), while the green/white cabbage and broccoli stem also yielded the essential fatty acid, linoleic acid (4). Brassica juncea (mustard) leaves and Raphanus sativus (radish) roots afforded 1, and the essential fatty acids 4 and α-linolenic acid (6). Mustard leaves also yielded trilinolenin (5), lutein (7) and β-carotene (8), while radish roots also afforded 2. These compounds were reported to exhibit anticancer properties.

Journal of Research in Science, Computing and Engineering, 2008

Der Pharmacia Lettre, Mar 2, 2015

Chemical investigation of the dichloromethane extract of freeze-dried Raphanus sativus roots led ... more Chemical investigation of the dichloromethane extract of freeze-dried Raphanus sativus roots led to the isolation of 4-methylthio-3-butenyl isothiocyanate or raphasatin (1a), 4-(methylthio)butyl isothiocyanate or erucin (1b), β-sitosterol (2) and unsaturated triglycerides (3). The structures of 1a and 1b were elucidated by extensive 1D and 2D NMR spectroscopy, while those of 2 and 3 were identified by comparison of their NMR data with those reported in the literature.

This work sought to develop a tissue culture method for Vitex negundo L., a popular medicinal her... more This work sought to develop a tissue culture method for Vitex negundo L., a popular medicinal herb with many known biological activities, and for which a number of biologically active metabolites have been identified and isolated from intact plant samples

Vitex negundo L. is a pantropic herb which can be found in South and Southeast Asia. Known locall... more Vitex negundo L. is a pantropic herb which can be found in South and Southeast Asia. Known locally in the Philippines as “lagundi”, V. negundo is a popular medicinal plant that is used as an antitussive, antiasthma and antipyretic (Quisumbing 1978, Pardo de Tavera 2000). A number of compounds have been isolated and identified from the leaves, including sesquiterpenes (β-eudesmol) (Dayrit & Trono 1995), flavonoids (casticin, chrysoplenol D, luteolin and isoorientin) (Dayrit et al.1987), iridoids (2’-para-hydroxybenzoyl mussaenosidic acid, 6’-parahydroxybenzoyl mussaenosidic acid, agnuside and lagundinin) (Dayrit & Lagurin 1994), and minor amounts of the plant steroid β-sitosterol (Joshi et al. 1974). Many other monoterpenes and sesquiterpenes had also been detected in smaller amounts by GC-MS (Mallavarapu et al. 1994). A callus line of Vitex negundo was established using explants from the leaves. The calli showed stable morphology and thin layer chromatographic (TLC) profiles over se...

Philippine Agriculturist, 1980

... Document details. Title The chemical and biochemical composition of legume seeds. 1. Mungbean... more ... Document details. Title The chemical and biochemical composition of legume seeds. 1. Mungbean. Authors Rosario, RR Del; Lozano, Y.; Noel, MG Journal Philippine Agriculturist 1980 Vol. 63 No. 3 pp. 267-274 ISSN 0031-7454 Record Number 19811425851. Abstract. ...

KIMIKA

Glucosinolates in mustard leaves (Brassica juncea L.) and seeds (Brassica nigra L.) were investig... more Glucosinolates in mustard leaves (Brassica juncea L.) and seeds (Brassica nigra L.) were investigated via chemical analysis of their major components and the activity of their hydrolysis products. High-performance liquid chromatography (HPLC) revealed that raw seeds have the highest amount of glucosinolates among the samples tested. Boiling of mustard leaves for 15 minutes significantly reduced the total glucosinolate content (10.94 mmol/g) to up to ~80% in comparison with the raw sample (54.31 mmol/g) which is attributed to thermal degradation of glucosinolates or leaching out to the boiling medium. Similarly, roasting of mustard seeds resulted in a marked decline in total glucosinolate content to 33.70 µmol/g compared with that of raw seed samples (86.18 µmol/g) due to thermal degradation. Liquid chromatography-mass spectrometry (LC-MS) revealed that sinigrin (allyl glucosinolate) is the major glucosinolate in mustard leaves, while the seeds contain sinigrin and gluconapin. Allyl ...

Pharmacognosy Journal

Materials Certification that the mature tubers of several R. sativus varieties used in this study... more Materials Certification that the mature tubers of several R. sativus varieties used in this study were of the same family and species was verified at the National Museum, Botany Division in Manila, Philippines. Extractions and analysis were done immediately to ensure reliability of results. Visual appearance and freedom from defects were considered in the selection of the samples for analyses. Sample preparation In accordance with common culinary practices, white radish tubers were washed and peeled before ABSTRACT This research was conducted to assess the gluocosinolate (GSL), isothiocyanate (ITC) and myrosinase content in two cultivars of Raphanus sativus Linn. (white and red) roots. LC-ESI-MS investigation was done on desulfated GSLs since this methodology has been previously established for efficient GSL analyses. The major GSLs: sinigrin (1) 1,2-dihydroxy-2-phenylethyl glucosinolate (2), 4-hydroxyglucobrassicin (3), glucoraphasatin (4) and 4-methoxyglucobrassicin (5) were found in red radishes; whereas, only 1 and 4 were obtained in white radishes. Myrosinase was analyzed in the tubers due to its ability to catalyze and hydrolyze GSLs into ITCs. This β-thioglucosidase enzyme was found to be over 10 times more active in red tubers (2.05E-02 units) than in white radishes (1.55E-03 units) and the results were linked to the presence/absence of the outer covering of the tubers. Due to the promising medicinal properties of the aglucone derivative of compound 4, 4-methylthio-3-butenyl isothiocyanate (6), the ITC analog was monitored using gas chromatographic mass spectral analyses after myrosinase-mediated hydrolysis. From the results, it can be construed that the occurrence of GSLs 1-5 and the bioactive agent 6 were inherent in the R. sativus cultivars evaluated.

The dichloromethane extracts of the leaves of Brassica oleracea var capitata f. rubra L (red cabb... more The dichloromethane extracts of the leaves of Brassica oleracea var capitata f. rubra L (red cabbage) and Brassica oleracea L (green/white cabbage) and the stem of Brassica oleracea L var. italic (broccoli) afforded β-sitosterol (1) and unsaturated triglycerides (2). The red cabbage also afforded stigmasterol (3), while the green/white cabbage and broccoli stem also yielded the essential fatty acid, linoleic acid (4). Brassica juncea (mustard) leaves and Raphanus sativus (radish) roots afforded 1, and the essential fatty acids 4 and α-linolenic acid (6). Mustard leaves also yielded trilinolenin (5), lutein (7) and β-carotene (8), while radish roots also afforded 2. These compounds were reported to exhibit anticancer properties.

The dichloromethane extracts of the leaves of Brassica oleracea var capitata f. rubra L (red cabb... more The dichloromethane extracts of the leaves of Brassica oleracea var capitata f. rubra L (red cabbage) and Brassica oleracea L (green/white cabbage) and the stem of Brassica oleracea L var. italic (broccoli) afforded β-sitosterol (1) and unsaturated triglycerides (2). The red cabbage also afforded stigmasterol (3), while the green/white cabbage and broccoli stem also yielded the essential fatty acid, linoleic acid (4). Brassica juncea (mustard) leaves and Raphanus sativus (radish) roots afforded 1, and the essential fatty acids 4 and α-linolenic acid (6). Mustard leaves also yielded trilinolenin (5), lutein (7) and β-carotene (8), while radish roots also afforded 2. These compounds were reported to exhibit anticancer properties.

Journal of Research in Science, Computing and Engineering, 2008

Der Pharmacia Lettre, Mar 2, 2015

Chemical investigation of the dichloromethane extract of freeze-dried Raphanus sativus roots led ... more Chemical investigation of the dichloromethane extract of freeze-dried Raphanus sativus roots led to the isolation of 4-methylthio-3-butenyl isothiocyanate or raphasatin (1a), 4-(methylthio)butyl isothiocyanate or erucin (1b), β-sitosterol (2) and unsaturated triglycerides (3). The structures of 1a and 1b were elucidated by extensive 1D and 2D NMR spectroscopy, while those of 2 and 3 were identified by comparison of their NMR data with those reported in the literature.