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Papers by Mary Kraft
Tissue Engineering Part C-methods, Jun 1, 2018
Analytical Chemistry, Aug 24, 2022
The FASEB Journal, Apr 1, 2012
The FASEB Journal, Apr 1, 2012
The FASEB Journal, Apr 1, 2012
The FASEB Journal, Apr 1, 2013
Organization of the plasma membranes’ of mammalian cells into domains of differing protein in lip... more Organization of the plasma membranes’ of mammalian cells into domains of differing protein in lipid composition is likely important for coordinating cell signaling and the numerous other processes that occur at the cell surface. By using a high‐resolution secondary ion mass spectrometry (SIMS) approach to chemically image the distributions of metabolically incorporated 15N‐sphingolipids in the plasma membranes of intact fibroblast cells, we previously showed that sphingolipids are non‐randomly organized in the plasma membrane. We now use this approach to test the mechanisms that are most often hypothesized to be responsible for sphingolipid distribution in the plasma membrane. We show that the non‐random sphingolipid organization within the plasma membrane cannot be attributed to favorable interactions with cholesterol. Instead, our results indicate that intracellular vesicle traffic and the cytoskeleton are responsible for the sphingolipid domains we observed.
The FASEB Journal
Organization of the plasma membranes’ of mammalian cells into domains of differing protein in lip... more Organization of the plasma membranes’ of mammalian cells into domains of differing protein in lipid composition is likely important for coordinating cell signaling and the numerous other processes that occur at the cell surface. By using a high‐resolution secondary ion mass spectrometry (SIMS) approach to chemically image the distributions of metabolically incorporated 15N‐sphingolipids in the plasma membranes of intact fibroblast cells, we previously showed that sphingolipids are non‐randomly organized in the plasma membrane. We now use this approach to test the mechanisms that are most often hypothesized to be responsible for sphingolipid distribution in the plasma membrane. We show that the non‐random sphingolipid organization within the plasma membrane cannot be attributed to favorable interactions with cholesterol. Instead, our results indicate that intracellular vesicle traffic and the cytoskeleton are responsible for the sphingolipid domains we observed.
Biophysical Journal, 2021
Biophysical Journal, 2022
The FASEB Journal, 2019
The influenza virus is hypothesized to assemble and bud from plasma membrane domains that are enr... more The influenza virus is hypothesized to assemble and bud from plasma membrane domains that are enriched with cholesterol and sphingolipids. This hypothesis is consistent with the reported enrichment of the influenza viral envelope with cholesterol and sphingolipids, the reduction in influenza virus protein clustering in the plasma membrane induced by cholesterol depletion, and the sensitivity of influenza virus formation to cellular cholesterol levels. Nonetheless, the levels of cholesterol and sphingolipids in the plasma membrane at the site of influenza virus budding have never been directly measured. The main challenge has been a lack of analytical methods with the sensitivity and lateral resolution that is required to measure the relative cholesterol and sphingolipid levels in the plasma membrane on the 100‐nm‐length scales that are relevant to influenza virus budding. We recently overcame this challenge by using a secondary ion mass spectrometry (SIMS) technique with high spatia...
The FASEB Journal, 2013
Bioactive sphingolipids are located and transported in cells according to their metabolic pathway... more Bioactive sphingolipids are located and transported in cells according to their metabolic pathway and cellular signaling events. However, absence of appropriate fluorescent tool has impeded understanding dynamic nature of sphingolipids during cellular function. To study sphingolipids trafficking and transport in live cells, it is the most desirable to use a fluorescent analog that follows native sphingolipid metabolism and transport, has high photostability, can be visualized in parallel with other common fluorescent labels, such as green fluorescent protein, and does not effect on cell viability. For this purpose, we recently reported a new fluorescent sphingosine, BODIPY 540 sphingosine, which satisfies these requirements. Here we use BODIPY 540 sphingosine to visualize the intracellular transport of fluorescent sphingosine and its fluorescent metabolites over time. We report the subcellular distribution of sphingolipids and their metabolites in living cells as a function of time....
This dataset consists of the secondary ion mass spectrometry (SIMS) depth profiling data that was... more This dataset consists of the secondary ion mass spectrometry (SIMS) depth profiling data that was collected with a Cameca NanoSIMS 50 instrument from a 10 micron by 10 micron region on a Madin-Darby canine kidney (MDCK) cell that had been metabolically labeled so most of its sphingolipids and cholesterol contained the rare nitrogen-15 oxygen-18 isotopes, respectively.
Tissue Engineering Part C-methods, Jun 1, 2018
Analytical Chemistry, Aug 24, 2022
The FASEB Journal, Apr 1, 2012
The FASEB Journal, Apr 1, 2012
The FASEB Journal, Apr 1, 2012
The FASEB Journal, Apr 1, 2013
Organization of the plasma membranes’ of mammalian cells into domains of differing protein in lip... more Organization of the plasma membranes’ of mammalian cells into domains of differing protein in lipid composition is likely important for coordinating cell signaling and the numerous other processes that occur at the cell surface. By using a high‐resolution secondary ion mass spectrometry (SIMS) approach to chemically image the distributions of metabolically incorporated 15N‐sphingolipids in the plasma membranes of intact fibroblast cells, we previously showed that sphingolipids are non‐randomly organized in the plasma membrane. We now use this approach to test the mechanisms that are most often hypothesized to be responsible for sphingolipid distribution in the plasma membrane. We show that the non‐random sphingolipid organization within the plasma membrane cannot be attributed to favorable interactions with cholesterol. Instead, our results indicate that intracellular vesicle traffic and the cytoskeleton are responsible for the sphingolipid domains we observed.
The FASEB Journal
Organization of the plasma membranes’ of mammalian cells into domains of differing protein in lip... more Organization of the plasma membranes’ of mammalian cells into domains of differing protein in lipid composition is likely important for coordinating cell signaling and the numerous other processes that occur at the cell surface. By using a high‐resolution secondary ion mass spectrometry (SIMS) approach to chemically image the distributions of metabolically incorporated 15N‐sphingolipids in the plasma membranes of intact fibroblast cells, we previously showed that sphingolipids are non‐randomly organized in the plasma membrane. We now use this approach to test the mechanisms that are most often hypothesized to be responsible for sphingolipid distribution in the plasma membrane. We show that the non‐random sphingolipid organization within the plasma membrane cannot be attributed to favorable interactions with cholesterol. Instead, our results indicate that intracellular vesicle traffic and the cytoskeleton are responsible for the sphingolipid domains we observed.
Biophysical Journal, 2021
Biophysical Journal, 2022
The FASEB Journal, 2019
The influenza virus is hypothesized to assemble and bud from plasma membrane domains that are enr... more The influenza virus is hypothesized to assemble and bud from plasma membrane domains that are enriched with cholesterol and sphingolipids. This hypothesis is consistent with the reported enrichment of the influenza viral envelope with cholesterol and sphingolipids, the reduction in influenza virus protein clustering in the plasma membrane induced by cholesterol depletion, and the sensitivity of influenza virus formation to cellular cholesterol levels. Nonetheless, the levels of cholesterol and sphingolipids in the plasma membrane at the site of influenza virus budding have never been directly measured. The main challenge has been a lack of analytical methods with the sensitivity and lateral resolution that is required to measure the relative cholesterol and sphingolipid levels in the plasma membrane on the 100‐nm‐length scales that are relevant to influenza virus budding. We recently overcame this challenge by using a secondary ion mass spectrometry (SIMS) technique with high spatia...
The FASEB Journal, 2013
Bioactive sphingolipids are located and transported in cells according to their metabolic pathway... more Bioactive sphingolipids are located and transported in cells according to their metabolic pathway and cellular signaling events. However, absence of appropriate fluorescent tool has impeded understanding dynamic nature of sphingolipids during cellular function. To study sphingolipids trafficking and transport in live cells, it is the most desirable to use a fluorescent analog that follows native sphingolipid metabolism and transport, has high photostability, can be visualized in parallel with other common fluorescent labels, such as green fluorescent protein, and does not effect on cell viability. For this purpose, we recently reported a new fluorescent sphingosine, BODIPY 540 sphingosine, which satisfies these requirements. Here we use BODIPY 540 sphingosine to visualize the intracellular transport of fluorescent sphingosine and its fluorescent metabolites over time. We report the subcellular distribution of sphingolipids and their metabolites in living cells as a function of time....
This dataset consists of the secondary ion mass spectrometry (SIMS) depth profiling data that was... more This dataset consists of the secondary ion mass spectrometry (SIMS) depth profiling data that was collected with a Cameca NanoSIMS 50 instrument from a 10 micron by 10 micron region on a Madin-Darby canine kidney (MDCK) cell that had been metabolically labeled so most of its sphingolipids and cholesterol contained the rare nitrogen-15 oxygen-18 isotopes, respectively.