Artemio Mendoza - Academia.edu (original) (raw)
Papers by Artemio Mendoza
Biocontrol Science and Technology, Jan 17, 2023
SSRN Electronic Journal, 2022
The Skn7, Ssk1 and Rim15 proteins are response regulators involved in osmotic, oxidative and nutr... more The Skn7, Ssk1 and Rim15 proteins are response regulators involved in osmotic, oxidative and nutritional stress in fungi. In order to verify the involvement of these genes in Trichoderma atroviride IMI206040's growth, conidiation, direct antagonism against plant pathogens (Rhizoctonia solani and Sclerotinia sclerotiorum), production of volatile organic compounds (VOCs) with fungistatic effect, and interaction with plants (growth promotion), single mutants were generated, and the phenotypic patterns were analysed in comparison to the wild-type (wt) strain. The mutants were submitted to osmotic, oxidative, membrane and cell wall stress conditions in vitro. The Δskn7 and Δrim15 mutants did not show either significant differences at morphological level, or marked decreases in mycelial growth and conidiation in relation to wt, whereas Δssk1 had altered phenotypes in most conditions tested. The plant-growth promotion of Arabidopsis thaliana seedlings induced by VOCs was not quantitatively modified by any of the mutants in relation to the wt strain, although possible differences in secondary root hairs was noticed for Δrim15. The fungistatic activity was significantly altered for Δssk1 and Δrim15. Overall, the Δssk1 strain showed remarkable morphological differences, with decrease in mycelial growth and conidiation, being also affected in the antagonistic capacity against plant pathogens. The impacts demonstrated by the deletion of ssk1 suggest this gene has a relevant participation in the signalling response to different stresses in T. atroviride and in the interactive metabolism with phytopathogens and plants. On the other hand, unlike other fungal models, Skn7 did not appear to have a critical participation in the above-mentioned processes; Rim15 seemed to confirm its involvement in modulating cellular responses to nutritional status, although with a possible cross-talk with other cellular processes. Our results suggest that Ssk1 likely plays a key regulatory role, not only in basic metabolisms of T. atroviride, but also in biocontrol-related characteristics.
Journal of Fungi
Trichoderma spp. produce multiple bioactive volatile organic compounds (VOCs). While the bioactiv... more Trichoderma spp. produce multiple bioactive volatile organic compounds (VOCs). While the bioactivity of VOCs from different Trichoderma species is well documented, information on intraspecific variation is limited. The fungistatic activity of VOCs emitted by 59 Trichoderma sp. “atroviride B” isolates against the pathogen Rhizoctonia solani was investigated. Eight isolates representing the two extremes of bioactivity against R. solani were also assessed against Alternaria radicina, Fusarium oxysporum f. sp. lycopersici and Sclerotinia sclerotiorum. VOCs profiles of these eight isolates were analyzed using gas chromatography–mass spectrometry (GC-MS) to identify a correlation between specific VOCs and bioactivity, and 11 VOCs were evaluated for bioactivity against the pathogens. Bioactivity against R. solani varied among the fifty-nine isolates, with five being strongly antagonistic. All eight selected isolates inhibited the growth of all four pathogens, with bioactivity being lowest ...
![Research paper thumbnail of Corrigendum to “Molecular dialogues between Trichoderma and roots: Role of the fungal secretome” [Fungal Biol Rev 32 (2018) 62–85]](https://attachments.academia-assets.com/99637182/thumbnails/1.jpg)
](Fungal Biology Reviews, 2019
Frontiers in Life Science, 2016
The definition of epigenetics is still under intense debate; however, its concept has evolved sin... more The definition of epigenetics is still under intense debate; however, its concept has evolved since it was originally introduced in 1939 by Conrad Hal Waddington as a way to reconcile antagonistic views between the school of preformationism and the school of epigenesis. The characterization of a large number of phenomena that diverge from the dogmas of classical genetics, and the discovery of the molecular mechanisms through which these phenomena occur, has given rise to a new area of study with important implications for biological sciences. Interactions between the environment and the DNA through modifications on the chromatin are not only responsible for the expression of a normal phenotype, these may be involved in the development of various pathologies. The epigenome, as the bridge between the genome and the phenotype, is no doubt one of the most interesting current ideas in genetics and is so revolutionary that it may change our present notions about inheritance and evolution. In this review, we made a compilation of the most important events in the history of epigenetics, its implications and some perspectives to the future.
Trichoderma: biology and applications, 2013
This chapter describes asexual reproduction strategies in Trichoderma spp. (conidiation, microcyc... more This chapter describes asexual reproduction strategies in Trichoderma spp. (conidiation, microcycle conidiation and chlamydosporogenesis), and discusses the roles of some factors on the induction of asexual reproduction in these organisms (light, biological rhythms, C/N status, pH, Ca2+ signalling, and volatile organic compounds).
Fungal Biology Reviews, 2022
30th Fungal Genetics Conference, Mar 1, 2019
Trichoderma species are cosmopolitan filamentous fungi found in agricultural, native prairie, for... more Trichoderma species are cosmopolitan filamentous fungi found in agricultural, native prairie, forest, salt marsh, and desert soils of all biomes (rainforests, savannas, deserts, grasslands, temperate deciduous forest, temperate, conifer forest, Mediterranean scrub, taiga and tundra), as well as in lake water, dead plant material, living roots of virtually any plant species, seeds and air (Atanasova et al., 2013; Mukherjee et al., 2013; Waghund et al., 2016). The ability of Trichoderma spp. to thrive in such a wide range of habitats is linked to their capability to produce a number of bioactive molecules, such as lytic enzymes, antibiotics and multiple other secondary metabolites. Rhizosphere competency is widespread among the Trichoderma and many strains are considered opportunistic plant endophytes frequently found in symbiotic relationships with diverse crops (including maize, tomato, cucumber, cotton, cocoa, etc.), ornamental flowers, grasses, palms, ferns, trees, etc. (Harman, 2...
Plant–microbe symbiosis is one of the most remarkable interactions in nature. Symbiotic microbes ... more Plant–microbe symbiosis is one of the most remarkable interactions in nature. Symbiotic microbes improve plant growth, increase disease resistance, and improve abiotic stress tolerance, in return for nutrients. Fungi of the genus Trichoderma establish beneficial symbiotic relationships with agriculturally relevant plants. They are found in soils worldwide and have a range of lifestyles that bring them into interaction with many different plant species, where a constant chemical communication may occur between plants and Trichoderma. The exact mechanism by which Trichoderma mask their entrance into their plant host is still unknown but likely involves the secretion of a plethora of chemical signals, including volatile organic compounds, toxins, and small peptides. This chapter summarizes current knowledge about the complex molecular interplay that occurs during communication between the host and invader, and the suppression of plant immune responses by Trichoderma.
Advances in PGPR research, 2017
This chapter discusses extremophile microorganisms, which have recently drawn attention for their... more This chapter discusses extremophile microorganisms, which have recently drawn attention for their unique adaptability mechanisms to tolerate extreme environmental changes. Nevertheless, a more comprehensive understanding of the genetics, biochemistry and physiology of these organisms is necessary to fully exploit their potential for bioremediating farmland, and promoting growth and reducing losses of crops.
European Journal of Organic Chemistry, 2020
Frontiers in Microbiology, 2019
Cruz-Magalhães et al. NOXs Regulate VOCs in Trichoderma in the blends emitted by the strains was ... more Cruz-Magalhães et al. NOXs Regulate VOCs in Trichoderma in the blends emitted by the strains was performed by GC-MS and the patterns of variation observed for individual volatiles, such as 6-Pentyl-2H-pyran-2-one (6PP-1) and (E)-6-Pent-1-enylpyran-2-one (6PP-2) were consistent with their negative effects in plant-growth promotion and positive effects in fungistasis at a distance. Nox1 and NoxR appear to have a ubiquitous regulatory role of in a variety of developmental and interactive processes in T. atroviride either as positive or negative modulators. Nox2 may also have a role in regulating production of VOCs with fungistatic activity.
Fungal Biology Reviews, 2018
Frontiers in Microbiology, 2018
Entomopathogenic fungi from the genus Beauveria (Vuillemin) play an important role in controlling... more Entomopathogenic fungi from the genus Beauveria (Vuillemin) play an important role in controlling insect populations and have been increasingly utilized for the biological control of insect pests. Various studies have reported that Beauveria bassiana (Bals.), Vuill. also has the ability to colonize a broad range of plant hosts as endophytes without causing disease but while still maintaining the capacity to infect insects. Beauveria is often applied as an inundative spore application, but little research has considered how plant colonization may alter the ability to persist in the environment. The aim of this study was to investigate potential interactions between B. bassiana and Zea mays L. (maize) in the rhizosphere following inoculation, in order to understand the factors that may affect environmental persistence of the fungi. The hypothesis was that different isolates of B. bassiana have the ability to colonize maize roots and/or rhizosphere soil, resulting in effects to the plant microbiome. To test this hypothesis, a two-step nested PCR protocol was developed to find and amplify Beauveria in planta or in soil; based on the translation elongation factor 1-alpha (ef1α) gene. The nested protocol was also designed to enable Beauveria species differentiation by sequence analysis. The impact of three selected B. bassiana isolates applied topically to roots on the rhizosphere soil community structure and function were consequently assessed using denaturing gradient gel electrophoresis (DGGE) and MicroResp TM techniques. The microbial community structure and function were not significantly affected by the presence of the isolates, however, retention of the inocula in the rhizosphere at 30 days after inoculation was enhanced when plants were subjected to intensive wounding of foliage to crudely simulate herbivory. The plant defense response likely changed under wound stress resulting in the apparent recruitment of Beauveria in the rhizosphere, which may be an indirect defensive strategy against herbivory and/or the result of induced systemic susceptibility in maize enabling plant colonization.
Journal of Basic Microbiology, 2017
In this study we examined the influence of the ambient pH during morphogenesis on conidial yield ... more In this study we examined the influence of the ambient pH during morphogenesis on conidial yield of Trichoderma sp. "atroviride B" LU132 and T. hamatum LU593 and storage at low temperatures. The ambient pH of the growth media had a dramatic influence on the level of Trichoderma conidiation and this was dependent on the strain and growth media. On malt-extract agar, LU593 yield decreased with increasing pH (3-6), whereas yield increased with increasing pH for LU132. During solid substrate production the reverse was true for LU132 whereby yield decreased with increasing pH. The germination potential of the conidia decreased significantly over time in cold storage and the rate of decline was a factor of the strain, pH during morphogenesis, growth media, and storage temperature.
Methods in Molecular Biology, 2016
Arch Med Res, Nov 10, 1999
Biocontrol Science and Technology, Jan 17, 2023
SSRN Electronic Journal, 2022
The Skn7, Ssk1 and Rim15 proteins are response regulators involved in osmotic, oxidative and nutr... more The Skn7, Ssk1 and Rim15 proteins are response regulators involved in osmotic, oxidative and nutritional stress in fungi. In order to verify the involvement of these genes in Trichoderma atroviride IMI206040's growth, conidiation, direct antagonism against plant pathogens (Rhizoctonia solani and Sclerotinia sclerotiorum), production of volatile organic compounds (VOCs) with fungistatic effect, and interaction with plants (growth promotion), single mutants were generated, and the phenotypic patterns were analysed in comparison to the wild-type (wt) strain. The mutants were submitted to osmotic, oxidative, membrane and cell wall stress conditions in vitro. The Δskn7 and Δrim15 mutants did not show either significant differences at morphological level, or marked decreases in mycelial growth and conidiation in relation to wt, whereas Δssk1 had altered phenotypes in most conditions tested. The plant-growth promotion of Arabidopsis thaliana seedlings induced by VOCs was not quantitatively modified by any of the mutants in relation to the wt strain, although possible differences in secondary root hairs was noticed for Δrim15. The fungistatic activity was significantly altered for Δssk1 and Δrim15. Overall, the Δssk1 strain showed remarkable morphological differences, with decrease in mycelial growth and conidiation, being also affected in the antagonistic capacity against plant pathogens. The impacts demonstrated by the deletion of ssk1 suggest this gene has a relevant participation in the signalling response to different stresses in T. atroviride and in the interactive metabolism with phytopathogens and plants. On the other hand, unlike other fungal models, Skn7 did not appear to have a critical participation in the above-mentioned processes; Rim15 seemed to confirm its involvement in modulating cellular responses to nutritional status, although with a possible cross-talk with other cellular processes. Our results suggest that Ssk1 likely plays a key regulatory role, not only in basic metabolisms of T. atroviride, but also in biocontrol-related characteristics.
Journal of Fungi
Trichoderma spp. produce multiple bioactive volatile organic compounds (VOCs). While the bioactiv... more Trichoderma spp. produce multiple bioactive volatile organic compounds (VOCs). While the bioactivity of VOCs from different Trichoderma species is well documented, information on intraspecific variation is limited. The fungistatic activity of VOCs emitted by 59 Trichoderma sp. “atroviride B” isolates against the pathogen Rhizoctonia solani was investigated. Eight isolates representing the two extremes of bioactivity against R. solani were also assessed against Alternaria radicina, Fusarium oxysporum f. sp. lycopersici and Sclerotinia sclerotiorum. VOCs profiles of these eight isolates were analyzed using gas chromatography–mass spectrometry (GC-MS) to identify a correlation between specific VOCs and bioactivity, and 11 VOCs were evaluated for bioactivity against the pathogens. Bioactivity against R. solani varied among the fifty-nine isolates, with five being strongly antagonistic. All eight selected isolates inhibited the growth of all four pathogens, with bioactivity being lowest ...
Fungal Biology Reviews, 2019
Frontiers in Life Science, 2016
The definition of epigenetics is still under intense debate; however, its concept has evolved sin... more The definition of epigenetics is still under intense debate; however, its concept has evolved since it was originally introduced in 1939 by Conrad Hal Waddington as a way to reconcile antagonistic views between the school of preformationism and the school of epigenesis. The characterization of a large number of phenomena that diverge from the dogmas of classical genetics, and the discovery of the molecular mechanisms through which these phenomena occur, has given rise to a new area of study with important implications for biological sciences. Interactions between the environment and the DNA through modifications on the chromatin are not only responsible for the expression of a normal phenotype, these may be involved in the development of various pathologies. The epigenome, as the bridge between the genome and the phenotype, is no doubt one of the most interesting current ideas in genetics and is so revolutionary that it may change our present notions about inheritance and evolution. In this review, we made a compilation of the most important events in the history of epigenetics, its implications and some perspectives to the future.
Trichoderma: biology and applications, 2013
This chapter describes asexual reproduction strategies in Trichoderma spp. (conidiation, microcyc... more This chapter describes asexual reproduction strategies in Trichoderma spp. (conidiation, microcycle conidiation and chlamydosporogenesis), and discusses the roles of some factors on the induction of asexual reproduction in these organisms (light, biological rhythms, C/N status, pH, Ca2+ signalling, and volatile organic compounds).
Fungal Biology Reviews, 2022
30th Fungal Genetics Conference, Mar 1, 2019
Trichoderma species are cosmopolitan filamentous fungi found in agricultural, native prairie, for... more Trichoderma species are cosmopolitan filamentous fungi found in agricultural, native prairie, forest, salt marsh, and desert soils of all biomes (rainforests, savannas, deserts, grasslands, temperate deciduous forest, temperate, conifer forest, Mediterranean scrub, taiga and tundra), as well as in lake water, dead plant material, living roots of virtually any plant species, seeds and air (Atanasova et al., 2013; Mukherjee et al., 2013; Waghund et al., 2016). The ability of Trichoderma spp. to thrive in such a wide range of habitats is linked to their capability to produce a number of bioactive molecules, such as lytic enzymes, antibiotics and multiple other secondary metabolites. Rhizosphere competency is widespread among the Trichoderma and many strains are considered opportunistic plant endophytes frequently found in symbiotic relationships with diverse crops (including maize, tomato, cucumber, cotton, cocoa, etc.), ornamental flowers, grasses, palms, ferns, trees, etc. (Harman, 2...
Plant–microbe symbiosis is one of the most remarkable interactions in nature. Symbiotic microbes ... more Plant–microbe symbiosis is one of the most remarkable interactions in nature. Symbiotic microbes improve plant growth, increase disease resistance, and improve abiotic stress tolerance, in return for nutrients. Fungi of the genus Trichoderma establish beneficial symbiotic relationships with agriculturally relevant plants. They are found in soils worldwide and have a range of lifestyles that bring them into interaction with many different plant species, where a constant chemical communication may occur between plants and Trichoderma. The exact mechanism by which Trichoderma mask their entrance into their plant host is still unknown but likely involves the secretion of a plethora of chemical signals, including volatile organic compounds, toxins, and small peptides. This chapter summarizes current knowledge about the complex molecular interplay that occurs during communication between the host and invader, and the suppression of plant immune responses by Trichoderma.
Advances in PGPR research, 2017
This chapter discusses extremophile microorganisms, which have recently drawn attention for their... more This chapter discusses extremophile microorganisms, which have recently drawn attention for their unique adaptability mechanisms to tolerate extreme environmental changes. Nevertheless, a more comprehensive understanding of the genetics, biochemistry and physiology of these organisms is necessary to fully exploit their potential for bioremediating farmland, and promoting growth and reducing losses of crops.
European Journal of Organic Chemistry, 2020
Frontiers in Microbiology, 2019
Cruz-Magalhães et al. NOXs Regulate VOCs in Trichoderma in the blends emitted by the strains was ... more Cruz-Magalhães et al. NOXs Regulate VOCs in Trichoderma in the blends emitted by the strains was performed by GC-MS and the patterns of variation observed for individual volatiles, such as 6-Pentyl-2H-pyran-2-one (6PP-1) and (E)-6-Pent-1-enylpyran-2-one (6PP-2) were consistent with their negative effects in plant-growth promotion and positive effects in fungistasis at a distance. Nox1 and NoxR appear to have a ubiquitous regulatory role of in a variety of developmental and interactive processes in T. atroviride either as positive or negative modulators. Nox2 may also have a role in regulating production of VOCs with fungistatic activity.
Fungal Biology Reviews, 2018
Frontiers in Microbiology, 2018
Entomopathogenic fungi from the genus Beauveria (Vuillemin) play an important role in controlling... more Entomopathogenic fungi from the genus Beauveria (Vuillemin) play an important role in controlling insect populations and have been increasingly utilized for the biological control of insect pests. Various studies have reported that Beauveria bassiana (Bals.), Vuill. also has the ability to colonize a broad range of plant hosts as endophytes without causing disease but while still maintaining the capacity to infect insects. Beauveria is often applied as an inundative spore application, but little research has considered how plant colonization may alter the ability to persist in the environment. The aim of this study was to investigate potential interactions between B. bassiana and Zea mays L. (maize) in the rhizosphere following inoculation, in order to understand the factors that may affect environmental persistence of the fungi. The hypothesis was that different isolates of B. bassiana have the ability to colonize maize roots and/or rhizosphere soil, resulting in effects to the plant microbiome. To test this hypothesis, a two-step nested PCR protocol was developed to find and amplify Beauveria in planta or in soil; based on the translation elongation factor 1-alpha (ef1α) gene. The nested protocol was also designed to enable Beauveria species differentiation by sequence analysis. The impact of three selected B. bassiana isolates applied topically to roots on the rhizosphere soil community structure and function were consequently assessed using denaturing gradient gel electrophoresis (DGGE) and MicroResp TM techniques. The microbial community structure and function were not significantly affected by the presence of the isolates, however, retention of the inocula in the rhizosphere at 30 days after inoculation was enhanced when plants were subjected to intensive wounding of foliage to crudely simulate herbivory. The plant defense response likely changed under wound stress resulting in the apparent recruitment of Beauveria in the rhizosphere, which may be an indirect defensive strategy against herbivory and/or the result of induced systemic susceptibility in maize enabling plant colonization.
Journal of Basic Microbiology, 2017
In this study we examined the influence of the ambient pH during morphogenesis on conidial yield ... more In this study we examined the influence of the ambient pH during morphogenesis on conidial yield of Trichoderma sp. "atroviride B" LU132 and T. hamatum LU593 and storage at low temperatures. The ambient pH of the growth media had a dramatic influence on the level of Trichoderma conidiation and this was dependent on the strain and growth media. On malt-extract agar, LU593 yield decreased with increasing pH (3-6), whereas yield increased with increasing pH for LU132. During solid substrate production the reverse was true for LU132 whereby yield decreased with increasing pH. The germination potential of the conidia decreased significantly over time in cold storage and the rate of decline was a factor of the strain, pH during morphogenesis, growth media, and storage temperature.
Methods in Molecular Biology, 2016
Arch Med Res, Nov 10, 1999