Mostafa Halim - Academia.edu (original) (raw)

Papers by Mostafa Halim

Chinese Journal of Plastic and Reconstructive Surgery

The Egyptian Journal of Plastic and Reconstructive Surgery

The Egyptian Journal of Plastic and Reconstructive Surgery, 2021

The Egyptian Journal of Plastic and Reconstructive Surgery, 2020

Background: High-Intensity Focused Ultrasound (HIFU) is promising for improving skin laxity with ... more Background: High-Intensity Focused Ultrasound (HIFU) is promising for improving skin laxity with satisfactory results. However, there is no comparative study on its efficacy and safety, when compared to Laser Assisted Liposuction (LAL) which is a minimal invasive procedure. Aim of Study: This study aimed to compare both LAL versus HIFU for neck tightening and correction of the double chin among young females. Methodology: This prospective cross sectional study was conducted over a six months' period starting from January 2018 till July 2018, on 30 female patients for neck tightening and correction of the double chin. Results: The clinical improvement of the skin laxity in the areas of the neck and chin after both procedures, showed excellent improvement with nearly similar results via HIFU (60.00%) as well as LAL cases (66.66%). The degree of the participants' satisfaction was satisfactory and nearly close among cases of both groups. Both techniques can produce satisfactory results however HIFU is considered safer, more rapid, effective, and noninvasive procedure for neck tightening, to improve the skin laxity. Conclusion: LAL is considered suitable in selected patients, for neck and chin contouring to achieve improvement of the skin laxity and excess fat of the cervicomental zone and jowls.

Gut, 2010

Introduction Treatment of severe chronic constipation (CC) is suboptimal. This study evaluates th... more Introduction Treatment of severe chronic constipation (CC) is suboptimal. This study evaluates the combined efficacy results of prucalopride (PRU) in three identical pivotal, randomised, placebo (PLA)-controlled trials. The objective of each trial was to compare the efficacy and safety of a 12 week once daily treatment of 2 mg or 4 mg PRU with PLA in CC. Methods All three trials were of identical design with three parallel treatment groups: PLA, PRU 2 mg and PRU 4 mg. Treatment phase followed a 2-week run-in. Two trials were executed in the US and one in Europe, Canada, Australia and South Africa. The main inclusion criterion was #2 spontaneous complete bowel movements (SCBM) per week in combination with straining, sensation of incomplete evacuation or hard stools, at least 25% of the time. The primary efficacy parameter was the percentage of patients with an average of 3SCBM/weekoverthe12−weektreatmentperiod.Thetwomainsecondaryendpointswerethepercentageofpatientswithanaverageincreaseof3 SCBM/week over the 12-week treatment period. The two main secondary endpoints were the percentage of patients with an average increase of 3SCBM/weekoverthe12−weektreatmentperiod.Thetwomainsecondaryendpointswerethepercentageofpatientswithanaverageincreaseof1 SCBM/week and the percentage of patients with an improvement 1onthesatisfactionsubscaleofthevalidatedqualityoflifeinstrument,PAC−QOL.Otherendpointswerebasedonnumberof(S)BM,timetofirstBM,stoolconsistency,strainingandpatient−reportedglobalevaluationofseverityofconstipation.Dataanalysisfollowedintent−to−treat(ITT)principles.ResultsTotal1924ITTpatients,891 on the satisfaction subscale of the validated quality of life instrument, PAC-QOL. Other endpoints were based on number of (S) BM, time to first BM, stool consistency, straining and patient-reported global evaluation of severity of constipation. Data analysis followed intent-to-treat (ITT) principles. Results Total 1924 ITT patients, 89% female, average age 47 years, average duration of constipation w20 years, prior treatments rated inadequate for 83%. Main Complaints: Infrequent defecation (30%), abdominal bloating (24%), abdominal pain (14%), during 2-week run-in w57% of the patients had no SCBM, and average weekly number of SCBM was 0.47. In each individual trial the results for the primary endpoint and secondary endpoints were significantly better for both PRU groups compared to PLA. For the three trials combined, the percentage of patients with an average of 1onthesatisfactionsubscaleofthevalidatedqualityoflifeinstrument,PAC−QOL.Otherendpointswerebasedonnumberof(S)BM,timetofirstBM,stoolconsistency,strainingandpatient−reportedglobalevaluationofseverityofconstipation.Dataanalysisfollowedintent−to−treat(ITT)principles.ResultsTotal1924ITTpatients,893 SCBM per week over the 12 week treatment period (primary endpoint) were 11.3%, 23.6% end 24.7% for PLA, PRU 2 mg and PRU 4 mg, respectively (both p<0.001 PRU vs PLA); similar for subjects who rated their previous therapy as inadequate. Response was highest in week 1 of treatment and stable over the remaining 11 weeks. PRU 2 mg and 4 mg consistently showed significant differences with PLA on all other secondary endpoints. Except for higher incidences of diarrhoea, nausea and headache on first day of treatment, PRU was well tolerated. Conclusion Both 2 and 4 mg PRU significantly improve bowel function and associated symptoms in CC compared to PLA. Results are reflected consistently in all observed efficacy parameters, including QOL.

Saudi Medical Journal, Oct 1, 2006

U rolithiasis is a multi-factorial recurrent rapidly increasing problem of universal distribution... more U rolithiasis is a multi-factorial recurrent rapidly increasing problem of universal distribution that causes substantial morbidity. Therefore, it is commonly believed that biochemical studies alone are never complete but correlation of clinical and biochemical results with that of chemical composition and structure of the stones is of great value in the delineation of pathogenesis, clinical management, preventive measures and choice of treatment modality. Until the late eighties, urinary stone analysis relied more on chemical rather than physical analytic techniques. The latter were considered not optimum for the quantitative analysis 1,2 as it detects the constituent salts providing it is not less than 5% of the sample. 1,3 Chemical analytic techniques and classification of stones. The chemical analytic techniques detect the individual ions. 1,4 In urinary stone analysis, the hospital laboratories relied only on the simple qualitative and semi-quantitative chemical analytic techniques 5 which only give arbitrary information on stone composition. 6,7 However, many investigators

Comparative Biochemistry and Physiology Part B: Comparative Biochemistry, 1988

Rabbit globin mRNA poly(A) was translated in two cell-free synthesizing systems, rabbit reticuloc... more Rabbit globin mRNA poly(A) was translated in two cell-free synthesizing systems, rabbit reticulocyte lysate and wheat germ extract, to characterize the product released from the poly(A) tract during globin synthesis. 2. Kinetic studies indicate that the size of the cleaved nucleotide proves to be a monomer, as revealed by column chromatography on Sephadex G-100 or G-25. 3. Characterization of the monomer was accomplished by chromatography on DEAE-cellulose. Initially, 5 min post-translation, the monomer was ATP only; however, at later times ATP, ADP, AMP and adenosine were detected. 4. The two synthesizing systems differed in that globin mRNA poly(A) was translated at a faster rate in the wheat germ extract as revealed by the appearance of ATP, whereas AMP was detected sooner in the rabbit reticulocyte lysate. 5. The results indicate that the A unit released from the poly(A) tract during mRNA poly(A) translation is a monomer, and that these metabolites may play a role in controlling protein initiation via the released ATP.

International Journal of Biochemistry, 1993

1. The length of the poly(A) tail at the 3&#39;-end of mRNA may control protein synthesis by ... more 1. The length of the poly(A) tail at the 3&#39;-end of mRNA may control protein synthesis by bringing the 3&#39;-end in close proximity to the 5&#39;-end of the noncoding region as well as increasing the duration of mRNA translation by its binding to the poly(A) binding protein. 2. The rate-limiting step in the decay of the body of the message is the shortening of a long poly(A) tail during mRNA translation. The shortening of the poly(A) tail occurs during pre-elongation in the protein synthesis cycle. 3. The shortening of the poly(A) tail during mRNA translation may not involve RNase activity, however poly(A) binding protein seems to play a role, at least in part, in shortening of the poly(A) tail.

American Journal of Gastroenterology, 2011

Systemic sclerosis (SSc) is a chronic multi-system autoimmune disorder with gastrointestinal trac... more Systemic sclerosis (SSc) is a chronic multi-system autoimmune disorder with gastrointestinal tract (GIT) involvement in up to 90 % of patients and anorectal involvement occurs in up to 50 % of patients. The pathogenesis of gastrointestinal abnormalities may be both myogenic and neurogenic. We aimed to identify which anorectal physiological abnormalities correlate with clinical symptoms and thus understand the pathophysiology of anorectal involvement in SSc. METHODS: In total, 44 SSc patients (24 symptomatic (Sx) (fecal incontinence) and 20 asymptomatic (ASx)) and 20 incontinent controls (ICs) were studied. Patients underwent anorectal manometry, rectal mucosal blood fl ow (RMBF), rectal compliance (barostat), and rectoanal inhibitory refl ex assessment (RAIR). RESULTS: Anal squeeze pressure was lower in the IC group compared with both the ASx and Sx groups (IC: 46.95 (30-63.9)) vs. ASx: 104.6 (81-128.3) vs. (Sx: 121.4 (101.3-141.6); P < 0.05). Resting pressure was lower in the IC group. RMBF and rectal compliance did not differ between groups. Anal, but not rectal, sensory threshold, was signifi cantly attenuated in Sx patients (

International Journal of Biochemistry, 1991

A factor found in rabbit serum inhibits globin mRNA translation in vitro. 2. Inhibition of globin... more A factor found in rabbit serum inhibits globin mRNA translation in vitro. 2. Inhibition of globin mRNA translation has been demonstrated in a cell-free rabbit reticulocyte lysate. 3. The inactivation of globin mRNA translation is not attributed to either serum albumin or ribonuclease activities. 4. Dialyzing the inhibitor for 24 hr at 4°C does not result in the diminution of the inhibiting activity. However, the activity of the inhibitor is destroyed by heating to 70-8o"C for 5 min or by treatment with trypsin for 2 hr. 5. Ion exchange chromatography points to the inhibitor being a neutral protein, whereas, polyacrylamide gel electrophoresis reveals one major band with mol. wt 43 kDa. 6. The activity of the inhibiting material 3-fold greater in anemic serum than in normal serum. 7. These studies suggest that rabbit serum contains a protein inhibitor that may play a physiological role in regulating protein synthesis in red cells.

International Journal of Biochemistry, 1988

When rabbit globin mRNA was incubated with rabbit reticulocyte lysate in the presence of various ... more When rabbit globin mRNA was incubated with rabbit reticulocyte lysate in the presence of various concentrations of nucleotides, globin synthesis was inhibited or stimulated dependent on dose. 2. Pyrimidine nucleotides inhibited protein synthesis at 0.3 mM, whereas 2 mM of purine nucleotides were required to cause similar inhibition. 3. Adenosine mono-and diphosphate inhibited globin synthesis at a concentration of only 1 mM; however, the sequence is AMP r ADP > ATP. 4. Translation arrest by these nucleotides was instantaneous. 5. These results suggest that these nucleotides may provide a structural component for maintaining the integrity. the conformation of mRNA or of the messenger ribonucleoprotein (mRNP).

International Journal of Biochemistry, 1994

1. A radiopolyadenylated rabbit globin mRNA was treated with different concentrations of ribonucl... more 1. A radiopolyadenylated rabbit globin mRNA was treated with different concentrations of ribonuclease V1 from cobra venom. 2. The enzymatic digests were chromatographed on an aminophenylboronate-agarose column, which specifically captured the cap structure i.e. m7G(5&#39;) ppp (5&#39;) NmP. 3. When the capture fragment was chromatographed on a Sephadex G-100 column, its size was smaller than the native molecule and also bore radioactivity, i.e. a poly(A) tail. 4. These results provide evidence that the 5&#39; end (which encompasses the cap structure) of rabbit globin mRNA is hybridized and in close proximity to its 3&#39; end. 5. We conclude that this conformation is required for messenger translation efficiency.

Journal of Theoretical Biology, 1992

1. J Theor Biol. 1992 Dec 7;159(3):377-9. Comparison of translation in eukaryotic mode with trans... more 1. J Theor Biol. 1992 Dec 7;159(3):377-9. Comparison of translation in eukaryotic mode with translation in prokaryote mode. Rubin HN, Halim MN. PMID: 1296097 [PubMed - indexed for MEDLINE]. Publication Types: Comparative Study; Letter. MeSH Terms: ...

International Journal of Surgery, 2013

Characteristic curve analysis was used to establish accuracy, sensitivity and specificity compare... more Characteristic curve analysis was used to establish accuracy, sensitivity and specificity compared to pathological stage. Results: Of 425 patients, 83 met the inclusion criteria. Accuracy for T4 disease was 88.2% ERUS and 83.8% MRI. MRI had greater sensitivity than ERUS for nodal stage (69.2% vs 48%) but lower specificity (52.4% vs 69%). Post-chemotherapy MRI had greater accuracy (93.8%) and specificity (100%) for ypT0 disease than ERUS (84.6% and 91.7%). ERUS was more sensitive for nodal sterilisation (ypN0) than MRI (75% vs 37.8%), although accuracy was poor in both modalities (53.8% and 48.8%). Conclusion: ERUS is a valuable adjunct to MRI in local staging of rectal cancer and may be superior in detecting T4 disease and nodal response post-radiotherapy. Restaging of rectal cancer following chemoradiation continues to be a challenging problem.

International Journal of Radiation Oncology*Biology*Physics, 1976

Abstract Gel electrophoretic patterns of nuclear and cytoplasmic ribosomal RNA (rRNA) from livers... more Abstract Gel electrophoretic patterns of nuclear and cytoplasmic ribosomal RNA (rRNA) from livers of intact and adrenalectomized rats were studied after exposure to gamma radiation (1800 rad) and/or 5-fluoroorotic acid (5-FOA) treatment. By 6 hr after either 5-FOA ...

International Journal of Radiation Oncology*Biology*Physics, 1976

Abstract Newly synthesized RNA as measured by 30 min uptake of (6-14 C) orotic acid was determine... more Abstract Newly synthesized RNA as measured by 30 min uptake of (6-14 C) orotic acid was determined in various liver cell fractions of control, irradiated, 5-FOA treated and 5-FOA plus irradiated adrenalectomizedand intact rats. The level of RNA synthesis was elevated1 hr ...

International Journal of Radiation Biology and Related Studies in Physics, Chemistry and Medicine, 1977

The effect of whole-body irradiation on RNA synthesis in regenerating and non-growing livers was ... more The effect of whole-body irradiation on RNA synthesis in regenerating and non-growing livers was studied in intact and adrenalectomized rats. The animals were divided into four sub-groups: (1) control; (2) irradiation only; (3) partially-hepatectomized; and (4) irradiated partially-hepatectomized. Newly-synthesized RNA was determined by 30 or 40 min uptake of (6-14C) orotic acid. Both nuclear and polyribosomal RNA synthesis in regenerating livers of adrenalectomized rats were depressed below their control levels, regardless of whether irradiation was 2 hours before or 2 hours after partial hepatectomy. Specific radioactivity values of regenerating livers of adrenal intact and adrenalectomized rats were elevated above those of the non-growing livers from irradiated and unirradiated rats.

International Journal of Radiation Biology and Related Studies in Physics, Chemistry and Medicine, 1979

The possible role of induced endogenous glucocorticoid levels stimulated by ionizing radiation an... more The possible role of induced endogenous glucocorticoid levels stimulated by ionizing radiation and their subsequent effect on rat-liver RNA was investigated in adrenalectomized rats using an intraperitoneal injection of cortisone acetate immediately prior to exposure to gamma-radiation (1800 rad). Adrenalectomized rats were divided into four groups: (1) control rats, (2) irradiated rats, (3) nonirradiated rats treated with cortisone acetate (10 mg/rat), and (4) irradiated rats which received cortisone acetate immediately before irradiation. Newly synthesized RNA was measured by an intraperitoneal injection of 8 muCi (6-14C) orotic acid either immediately after irradiation or 40 min prior to killing. All the rats were killed 6 hours post-irradiation. The results of these investigations indicate that (1) newly synthesized (40 min labelling) RNA increased in irradiated adrenal-intact rats, but not in adrenalectomized animals; (2) cortisone acetate by itself stimulated RNA synthesis whether the radioactive precursor was administered 6 hours of 40 min prior to killing; and (3) irradiation of adrenalectomized rats pre-treated with cortisone acetate induced more RNA synthesis than did cortisone alone. The results of this study support the suggestion that ionizing radiation may induce rat-liver RNA synthesis, at least in part, via stimulated adrenal secretion of glucocorticoids.

International Journal of Radiation Biology, 1979

Newly synthesized messenger RNA, as measured by a 40 min uptake of the radioactive precursor (6-1... more Newly synthesized messenger RNA, as measured by a 40 min uptake of the radioactive precursor (6-14C) orotic acid, was studied in the regenerating livers of non-irradiated and gamma-irradiated (1800 rad) adrenal-intact and adrenalectomized rats 24 aand 48 hours after partial hepatectomy. Two groups of rats, one with and one without adrenal glands, were each divided into four subgroups: (1) control rats, (2) irradiated rats, (3) partially hepatectomized rats and (4) irradiated, partially hepatectomized rats. The radioactive profile of polyribosome formation and distribution was determined by sucrose density gradient centrifugation (10--40 per cent). The result of this study indicates that ionizing radiation decreases the synthesis of newly formed messenger RNA in re generating livers of adrenal-intact rats. However, adrenalectomy largely abolished that inhibition. These data suggest that the decrease in messenger RNA synthesis may be explained by the disturbance of adrenal hormones induced by partial hepatectomy and ionizing radiation.

Chinese Journal of Plastic and Reconstructive Surgery

The Egyptian Journal of Plastic and Reconstructive Surgery

The Egyptian Journal of Plastic and Reconstructive Surgery, 2021

The Egyptian Journal of Plastic and Reconstructive Surgery, 2020

Background: High-Intensity Focused Ultrasound (HIFU) is promising for improving skin laxity with ... more Background: High-Intensity Focused Ultrasound (HIFU) is promising for improving skin laxity with satisfactory results. However, there is no comparative study on its efficacy and safety, when compared to Laser Assisted Liposuction (LAL) which is a minimal invasive procedure. Aim of Study: This study aimed to compare both LAL versus HIFU for neck tightening and correction of the double chin among young females. Methodology: This prospective cross sectional study was conducted over a six months' period starting from January 2018 till July 2018, on 30 female patients for neck tightening and correction of the double chin. Results: The clinical improvement of the skin laxity in the areas of the neck and chin after both procedures, showed excellent improvement with nearly similar results via HIFU (60.00%) as well as LAL cases (66.66%). The degree of the participants' satisfaction was satisfactory and nearly close among cases of both groups. Both techniques can produce satisfactory results however HIFU is considered safer, more rapid, effective, and noninvasive procedure for neck tightening, to improve the skin laxity. Conclusion: LAL is considered suitable in selected patients, for neck and chin contouring to achieve improvement of the skin laxity and excess fat of the cervicomental zone and jowls.

Gut, 2010

Introduction Treatment of severe chronic constipation (CC) is suboptimal. This study evaluates th... more Introduction Treatment of severe chronic constipation (CC) is suboptimal. This study evaluates the combined efficacy results of prucalopride (PRU) in three identical pivotal, randomised, placebo (PLA)-controlled trials. The objective of each trial was to compare the efficacy and safety of a 12 week once daily treatment of 2 mg or 4 mg PRU with PLA in CC. Methods All three trials were of identical design with three parallel treatment groups: PLA, PRU 2 mg and PRU 4 mg. Treatment phase followed a 2-week run-in. Two trials were executed in the US and one in Europe, Canada, Australia and South Africa. The main inclusion criterion was #2 spontaneous complete bowel movements (SCBM) per week in combination with straining, sensation of incomplete evacuation or hard stools, at least 25% of the time. The primary efficacy parameter was the percentage of patients with an average of 3SCBM/weekoverthe12−weektreatmentperiod.Thetwomainsecondaryendpointswerethepercentageofpatientswithanaverageincreaseof3 SCBM/week over the 12-week treatment period. The two main secondary endpoints were the percentage of patients with an average increase of 3SCBM/weekoverthe12−weektreatmentperiod.Thetwomainsecondaryendpointswerethepercentageofpatientswithanaverageincreaseof1 SCBM/week and the percentage of patients with an improvement 1onthesatisfactionsubscaleofthevalidatedqualityoflifeinstrument,PAC−QOL.Otherendpointswerebasedonnumberof(S)BM,timetofirstBM,stoolconsistency,strainingandpatient−reportedglobalevaluationofseverityofconstipation.Dataanalysisfollowedintent−to−treat(ITT)principles.ResultsTotal1924ITTpatients,891 on the satisfaction subscale of the validated quality of life instrument, PAC-QOL. Other endpoints were based on number of (S) BM, time to first BM, stool consistency, straining and patient-reported global evaluation of severity of constipation. Data analysis followed intent-to-treat (ITT) principles. Results Total 1924 ITT patients, 89% female, average age 47 years, average duration of constipation w20 years, prior treatments rated inadequate for 83%. Main Complaints: Infrequent defecation (30%), abdominal bloating (24%), abdominal pain (14%), during 2-week run-in w57% of the patients had no SCBM, and average weekly number of SCBM was 0.47. In each individual trial the results for the primary endpoint and secondary endpoints were significantly better for both PRU groups compared to PLA. For the three trials combined, the percentage of patients with an average of 1onthesatisfactionsubscaleofthevalidatedqualityoflifeinstrument,PAC−QOL.Otherendpointswerebasedonnumberof(S)BM,timetofirstBM,stoolconsistency,strainingandpatient−reportedglobalevaluationofseverityofconstipation.Dataanalysisfollowedintent−to−treat(ITT)principles.ResultsTotal1924ITTpatients,893 SCBM per week over the 12 week treatment period (primary endpoint) were 11.3%, 23.6% end 24.7% for PLA, PRU 2 mg and PRU 4 mg, respectively (both p<0.001 PRU vs PLA); similar for subjects who rated their previous therapy as inadequate. Response was highest in week 1 of treatment and stable over the remaining 11 weeks. PRU 2 mg and 4 mg consistently showed significant differences with PLA on all other secondary endpoints. Except for higher incidences of diarrhoea, nausea and headache on first day of treatment, PRU was well tolerated. Conclusion Both 2 and 4 mg PRU significantly improve bowel function and associated symptoms in CC compared to PLA. Results are reflected consistently in all observed efficacy parameters, including QOL.

Saudi Medical Journal, Oct 1, 2006

U rolithiasis is a multi-factorial recurrent rapidly increasing problem of universal distribution... more U rolithiasis is a multi-factorial recurrent rapidly increasing problem of universal distribution that causes substantial morbidity. Therefore, it is commonly believed that biochemical studies alone are never complete but correlation of clinical and biochemical results with that of chemical composition and structure of the stones is of great value in the delineation of pathogenesis, clinical management, preventive measures and choice of treatment modality. Until the late eighties, urinary stone analysis relied more on chemical rather than physical analytic techniques. The latter were considered not optimum for the quantitative analysis 1,2 as it detects the constituent salts providing it is not less than 5% of the sample. 1,3 Chemical analytic techniques and classification of stones. The chemical analytic techniques detect the individual ions. 1,4 In urinary stone analysis, the hospital laboratories relied only on the simple qualitative and semi-quantitative chemical analytic techniques 5 which only give arbitrary information on stone composition. 6,7 However, many investigators

Comparative Biochemistry and Physiology Part B: Comparative Biochemistry, 1988

Rabbit globin mRNA poly(A) was translated in two cell-free synthesizing systems, rabbit reticuloc... more Rabbit globin mRNA poly(A) was translated in two cell-free synthesizing systems, rabbit reticulocyte lysate and wheat germ extract, to characterize the product released from the poly(A) tract during globin synthesis. 2. Kinetic studies indicate that the size of the cleaved nucleotide proves to be a monomer, as revealed by column chromatography on Sephadex G-100 or G-25. 3. Characterization of the monomer was accomplished by chromatography on DEAE-cellulose. Initially, 5 min post-translation, the monomer was ATP only; however, at later times ATP, ADP, AMP and adenosine were detected. 4. The two synthesizing systems differed in that globin mRNA poly(A) was translated at a faster rate in the wheat germ extract as revealed by the appearance of ATP, whereas AMP was detected sooner in the rabbit reticulocyte lysate. 5. The results indicate that the A unit released from the poly(A) tract during mRNA poly(A) translation is a monomer, and that these metabolites may play a role in controlling protein initiation via the released ATP.

International Journal of Biochemistry, 1993

1. The length of the poly(A) tail at the 3&#39;-end of mRNA may control protein synthesis by ... more 1. The length of the poly(A) tail at the 3&#39;-end of mRNA may control protein synthesis by bringing the 3&#39;-end in close proximity to the 5&#39;-end of the noncoding region as well as increasing the duration of mRNA translation by its binding to the poly(A) binding protein. 2. The rate-limiting step in the decay of the body of the message is the shortening of a long poly(A) tail during mRNA translation. The shortening of the poly(A) tail occurs during pre-elongation in the protein synthesis cycle. 3. The shortening of the poly(A) tail during mRNA translation may not involve RNase activity, however poly(A) binding protein seems to play a role, at least in part, in shortening of the poly(A) tail.

American Journal of Gastroenterology, 2011

Systemic sclerosis (SSc) is a chronic multi-system autoimmune disorder with gastrointestinal trac... more Systemic sclerosis (SSc) is a chronic multi-system autoimmune disorder with gastrointestinal tract (GIT) involvement in up to 90 % of patients and anorectal involvement occurs in up to 50 % of patients. The pathogenesis of gastrointestinal abnormalities may be both myogenic and neurogenic. We aimed to identify which anorectal physiological abnormalities correlate with clinical symptoms and thus understand the pathophysiology of anorectal involvement in SSc. METHODS: In total, 44 SSc patients (24 symptomatic (Sx) (fecal incontinence) and 20 asymptomatic (ASx)) and 20 incontinent controls (ICs) were studied. Patients underwent anorectal manometry, rectal mucosal blood fl ow (RMBF), rectal compliance (barostat), and rectoanal inhibitory refl ex assessment (RAIR). RESULTS: Anal squeeze pressure was lower in the IC group compared with both the ASx and Sx groups (IC: 46.95 (30-63.9)) vs. ASx: 104.6 (81-128.3) vs. (Sx: 121.4 (101.3-141.6); P < 0.05). Resting pressure was lower in the IC group. RMBF and rectal compliance did not differ between groups. Anal, but not rectal, sensory threshold, was signifi cantly attenuated in Sx patients (

International Journal of Biochemistry, 1991

A factor found in rabbit serum inhibits globin mRNA translation in vitro. 2. Inhibition of globin... more A factor found in rabbit serum inhibits globin mRNA translation in vitro. 2. Inhibition of globin mRNA translation has been demonstrated in a cell-free rabbit reticulocyte lysate. 3. The inactivation of globin mRNA translation is not attributed to either serum albumin or ribonuclease activities. 4. Dialyzing the inhibitor for 24 hr at 4°C does not result in the diminution of the inhibiting activity. However, the activity of the inhibitor is destroyed by heating to 70-8o"C for 5 min or by treatment with trypsin for 2 hr. 5. Ion exchange chromatography points to the inhibitor being a neutral protein, whereas, polyacrylamide gel electrophoresis reveals one major band with mol. wt 43 kDa. 6. The activity of the inhibiting material 3-fold greater in anemic serum than in normal serum. 7. These studies suggest that rabbit serum contains a protein inhibitor that may play a physiological role in regulating protein synthesis in red cells.

International Journal of Biochemistry, 1988

When rabbit globin mRNA was incubated with rabbit reticulocyte lysate in the presence of various ... more When rabbit globin mRNA was incubated with rabbit reticulocyte lysate in the presence of various concentrations of nucleotides, globin synthesis was inhibited or stimulated dependent on dose. 2. Pyrimidine nucleotides inhibited protein synthesis at 0.3 mM, whereas 2 mM of purine nucleotides were required to cause similar inhibition. 3. Adenosine mono-and diphosphate inhibited globin synthesis at a concentration of only 1 mM; however, the sequence is AMP r ADP > ATP. 4. Translation arrest by these nucleotides was instantaneous. 5. These results suggest that these nucleotides may provide a structural component for maintaining the integrity. the conformation of mRNA or of the messenger ribonucleoprotein (mRNP).

International Journal of Biochemistry, 1994

1. A radiopolyadenylated rabbit globin mRNA was treated with different concentrations of ribonucl... more 1. A radiopolyadenylated rabbit globin mRNA was treated with different concentrations of ribonuclease V1 from cobra venom. 2. The enzymatic digests were chromatographed on an aminophenylboronate-agarose column, which specifically captured the cap structure i.e. m7G(5&#39;) ppp (5&#39;) NmP. 3. When the capture fragment was chromatographed on a Sephadex G-100 column, its size was smaller than the native molecule and also bore radioactivity, i.e. a poly(A) tail. 4. These results provide evidence that the 5&#39; end (which encompasses the cap structure) of rabbit globin mRNA is hybridized and in close proximity to its 3&#39; end. 5. We conclude that this conformation is required for messenger translation efficiency.

Journal of Theoretical Biology, 1992

1. J Theor Biol. 1992 Dec 7;159(3):377-9. Comparison of translation in eukaryotic mode with trans... more 1. J Theor Biol. 1992 Dec 7;159(3):377-9. Comparison of translation in eukaryotic mode with translation in prokaryote mode. Rubin HN, Halim MN. PMID: 1296097 [PubMed - indexed for MEDLINE]. Publication Types: Comparative Study; Letter. MeSH Terms: ...

International Journal of Surgery, 2013

Characteristic curve analysis was used to establish accuracy, sensitivity and specificity compare... more Characteristic curve analysis was used to establish accuracy, sensitivity and specificity compared to pathological stage. Results: Of 425 patients, 83 met the inclusion criteria. Accuracy for T4 disease was 88.2% ERUS and 83.8% MRI. MRI had greater sensitivity than ERUS for nodal stage (69.2% vs 48%) but lower specificity (52.4% vs 69%). Post-chemotherapy MRI had greater accuracy (93.8%) and specificity (100%) for ypT0 disease than ERUS (84.6% and 91.7%). ERUS was more sensitive for nodal sterilisation (ypN0) than MRI (75% vs 37.8%), although accuracy was poor in both modalities (53.8% and 48.8%). Conclusion: ERUS is a valuable adjunct to MRI in local staging of rectal cancer and may be superior in detecting T4 disease and nodal response post-radiotherapy. Restaging of rectal cancer following chemoradiation continues to be a challenging problem.

International Journal of Radiation Oncology*Biology*Physics, 1976

Abstract Gel electrophoretic patterns of nuclear and cytoplasmic ribosomal RNA (rRNA) from livers... more Abstract Gel electrophoretic patterns of nuclear and cytoplasmic ribosomal RNA (rRNA) from livers of intact and adrenalectomized rats were studied after exposure to gamma radiation (1800 rad) and/or 5-fluoroorotic acid (5-FOA) treatment. By 6 hr after either 5-FOA ...

International Journal of Radiation Oncology*Biology*Physics, 1976

Abstract Newly synthesized RNA as measured by 30 min uptake of (6-14 C) orotic acid was determine... more Abstract Newly synthesized RNA as measured by 30 min uptake of (6-14 C) orotic acid was determined in various liver cell fractions of control, irradiated, 5-FOA treated and 5-FOA plus irradiated adrenalectomizedand intact rats. The level of RNA synthesis was elevated1 hr ...

International Journal of Radiation Biology and Related Studies in Physics, Chemistry and Medicine, 1977

The effect of whole-body irradiation on RNA synthesis in regenerating and non-growing livers was ... more The effect of whole-body irradiation on RNA synthesis in regenerating and non-growing livers was studied in intact and adrenalectomized rats. The animals were divided into four sub-groups: (1) control; (2) irradiation only; (3) partially-hepatectomized; and (4) irradiated partially-hepatectomized. Newly-synthesized RNA was determined by 30 or 40 min uptake of (6-14C) orotic acid. Both nuclear and polyribosomal RNA synthesis in regenerating livers of adrenalectomized rats were depressed below their control levels, regardless of whether irradiation was 2 hours before or 2 hours after partial hepatectomy. Specific radioactivity values of regenerating livers of adrenal intact and adrenalectomized rats were elevated above those of the non-growing livers from irradiated and unirradiated rats.

International Journal of Radiation Biology and Related Studies in Physics, Chemistry and Medicine, 1979

The possible role of induced endogenous glucocorticoid levels stimulated by ionizing radiation an... more The possible role of induced endogenous glucocorticoid levels stimulated by ionizing radiation and their subsequent effect on rat-liver RNA was investigated in adrenalectomized rats using an intraperitoneal injection of cortisone acetate immediately prior to exposure to gamma-radiation (1800 rad). Adrenalectomized rats were divided into four groups: (1) control rats, (2) irradiated rats, (3) nonirradiated rats treated with cortisone acetate (10 mg/rat), and (4) irradiated rats which received cortisone acetate immediately before irradiation. Newly synthesized RNA was measured by an intraperitoneal injection of 8 muCi (6-14C) orotic acid either immediately after irradiation or 40 min prior to killing. All the rats were killed 6 hours post-irradiation. The results of these investigations indicate that (1) newly synthesized (40 min labelling) RNA increased in irradiated adrenal-intact rats, but not in adrenalectomized animals; (2) cortisone acetate by itself stimulated RNA synthesis whether the radioactive precursor was administered 6 hours of 40 min prior to killing; and (3) irradiation of adrenalectomized rats pre-treated with cortisone acetate induced more RNA synthesis than did cortisone alone. The results of this study support the suggestion that ionizing radiation may induce rat-liver RNA synthesis, at least in part, via stimulated adrenal secretion of glucocorticoids.

International Journal of Radiation Biology, 1979

Newly synthesized messenger RNA, as measured by a 40 min uptake of the radioactive precursor (6-1... more Newly synthesized messenger RNA, as measured by a 40 min uptake of the radioactive precursor (6-14C) orotic acid, was studied in the regenerating livers of non-irradiated and gamma-irradiated (1800 rad) adrenal-intact and adrenalectomized rats 24 aand 48 hours after partial hepatectomy. Two groups of rats, one with and one without adrenal glands, were each divided into four subgroups: (1) control rats, (2) irradiated rats, (3) partially hepatectomized rats and (4) irradiated, partially hepatectomized rats. The radioactive profile of polyribosome formation and distribution was determined by sucrose density gradient centrifugation (10--40 per cent). The result of this study indicates that ionizing radiation decreases the synthesis of newly formed messenger RNA in re generating livers of adrenal-intact rats. However, adrenalectomy largely abolished that inhibition. These data suggest that the decrease in messenger RNA synthesis may be explained by the disturbance of adrenal hormones induced by partial hepatectomy and ionizing radiation.