Sayani Mukherjee - Academia.edu (original) (raw)

Uploads

Papers by Sayani Mukherjee

Research paper thumbnail of Induced Diarrhea in Suckling Mice

Journal of medicinal food

In this study, the extract of a green leafy vegetable Oxalis corniculata (Oxalidaceae) was evalua... more In this study, the extract of a green leafy vegetable Oxalis corniculata (Oxalidaceae) was evaluated for its in vitro antibacterial and in vivo anti colonizing effect against common intestinal pathogenic bacteria. Methanolic extract (80%) of Oxalis corniculata (Oxalidaceae) leaf contained a polyphenol content of 910 mg Gallic acid equivalent per gram of dry weight and the yield was 8%. The flavonoid content was 2.353 g quercetin equivalent per 100 g of the extract. In vitro studies indicated that the extract inhibited numerous pathogenic bacteria like Staphylococcus aureus (ATCC 25922), Escherichia coli (ATCC 25923), Shigella dysenteriae 1 (NT4907), Shigella flexneri 2a (2457T), Shigella boydii 4 (BCH612), and Shigella sonnie phase I (IDH00968). The minimum inhibitory concentration (MIC) against E. coli (ATCC 25923) was minimal (0.08 mg/mL), whereas MIC against S. flexneri 2a (2457T) was higher (0.13 mg/mL). A suckling mouse model was developed which involved challenging the mice in...

Research paper thumbnail of Comparative prophylactic effects of α- eleostearic acid rich nano and conventional emulsions in induced diabetic rats

This e-offprint is for personal use only and shall not be self-archived in electronic repositorie... more This e-offprint is for personal use only and shall not be self-archived in electronic repositories. If you wish to self-archive your article, please use the accepted manuscript version for posting on your own website. You may further deposit the accepted manuscript version in any repository, provided it is only made publicly available 12 months after official publication or later and provided acknowledgement is given to the original source of publication and a link is inserted to the published article on Springer's website. The link must be accompanied by the following text: "The final publication is available at link.springer.com".

Research paper thumbnail of Comparative real-time study of cellular uptake of a formulated conjugated linolenic acid rich nano and conventional macro emulsions and their bioactivity in ex vivo models for parenteral applications

The objective of the present study was to fabricate and monitor real-time, impact of a stable con... more The objective of the present study was to fabricate and monitor real-time, impact of a stable conjugated
linolenic acid, -eleostearic acid (ESA) rich nanoemulsion (NE) formulation (d < 200 nm) vis-à-vis ESA
conventional emulsion (CE) system in ex vivo systems against both endogenous and exogenous reactive
oxygen species (ROS). Accordingly, stable nanoemulsion formulation of ESA was engineered with
the aid of bitter melon seed oil and non-toxic excipients. Morphology and particle size of the emulsion
formulations were studied to validate the stability. The real-time rapid uptake of the ESA NE and its
increased prophylactic efficacy against induced endogenous and exogenous ROS in terms of cell viability
and membrane integrity was evaluated flow-cytometrically and with fluorescence microscopic analysis
of different primary cells.
It was found that the fabricated non-toxic ESA NE had stable parameters (hydrodynamic mean diameter,
particle size distribution and zeta potential) for over 12 weeks. Further, ESA NE at a concentration of
∼70 M exhibited maximum efficacy in protecting cells from oxidative damage against both endogenous
and exogenous ROS in lymphocytes and hepatocytes as compared to its corresponding presence in the
CE formulation.
This study provides a real-time empirical evidence on the influence of nano formulation in enhancing
bioavailability and antioxidative properties of ESA.

Research paper thumbnail of Oxalis corniculata (Oxalidaceae) Leaf Extract Exerts In Vitro Antimicrobial and In Vivo Anticolonizing Activities Against Shigella dysenteriae 1 (NT4907) and Shigella flexneri 2a (2457T) in Induced Diarrhea in Suckling Mice

Journal of Medicinal Food, 2013

In this study, the extract of a green leafy vegetable Oxalis corniculata (Oxalidaceae) was evalua... more In this study, the extract of a green leafy vegetable Oxalis corniculata (Oxalidaceae) was evaluated for its in vitro antibacterial and in vivo anti colonizing effect against common intestinal pathogenic bacteria. Methanolic extract (80%) of Oxalis corniculata (Oxalidaceae) leaf contained a polyphenol content of 910 mg Gallic acid equivalent per gram of dry weight and the yield was 8%. The flavonoid content was 2.353 g quercetin equivalent per 100 g of the extract. In vitro studies indicated that the extract inhibited numerous pathogenic bacteria like Staphylococcus aureus (ATCC 25922), Escherichia coli (ATCC 25923), Shigella dysenteriae 1 (NT4907), Shigella flexneri 2a (2457T), Shigella boydii 4 (BCH612), and Shigella sonnie phase I (IDH00968). The minimum inhibitory concentration (MIC) against E. coli (ATCC 25923) was minimal (0.08 mg/mL), whereas MIC against S. flexneri 2a (2457T) was higher (0.13 mg/mL). A suckling mouse model was developed which involved challenging the mice intragastrically with S. flexneri 2a (2457T) and S. dysenteriae 1 (NT4907) to study the anticolonization activity. It was revealed that the extract was more potent against S. dysenteriae 1 (NT4907) as compared to S. flexneri 2a (2457T). It was also found that simultaneous administration of extract along with bacterial inoculums promoted good anticolonization activity. Significant activity was observed even when treated after 3 h of bacterial inoculation.

Research paper thumbnail of Evaluation of antioxidative, antibacterial and probiotic growth stimulatory activities of Sesamum indicum honey containing phenolic compounds and lignans

LWT - Food Science and Technology, 2015

Seven Sesamum indicum (sesame) honey samples were collected from Hooghly district of West Bengal,... more Seven Sesamum indicum (sesame) honey samples were collected from Hooghly district of West Bengal, India and analyzed for polyphenol and flavonoid content along with their in vitro free radical scavenging activities. Antibacterial activity and stimulatory effect on multiplication of probiotic bacteria were evaluated. Antioxidant markers like IC 50 value for DPPH (1, 1-diphenyl-2-picrylhydrazyl) and FRAP (ferric reducing antioxidant power) of sesame honey were positively correlated to its polyphenolic content (28.9 ± 0.6 mg GAE/100 g) and color intensity (r ranges between 0.872 and 0.931). Four flavonoids viz., apigenin, quercetin, myricetin, rutin have been identified along with one cinnamic acid derivative (ferulic acid) and two sesame lignans (sesamin and episesamin) by High performance liquid chromatography which can be used as a tool for authentication of sesame honey. Antibacterial activity of sesame honey against some enteropathogenic bacteria Escherichia coli, Vibrio cholerae, S. Typhi, S. Typhimurium were studied. Minimum inhibitory concentration was found to be lowest against S. Typhi (12.5% w/v) and S. Typhimurium (12.5% w/v). Plasmid DNA degradation by sesame honey administration evinced its molecular level of action. Interestingly, it was also found that sesame honey exhibited significant growth promoting property of probiotic strains such as Lactobacillus acidophilus and Bifidobacterium bifidum.

Research paper thumbnail of Comparative prophylactic effects of α-eleostearic acid rich nano and conventional emulsions in induced diabetic rats

Journal of Food Science and Technology, 2014

This e-offprint is for personal use only and shall not be self-archived in electronic repositorie... more This e-offprint is for personal use only and shall not be self-archived in electronic repositories. If you wish to self-archive your article, please use the accepted manuscript version for posting on your own website. You may further deposit the accepted manuscript version in any repository, provided it is only made publicly available 12 months after official publication or later and provided acknowledgement is given to the original source of publication and a link is inserted to the published article on Springer's website. The link must be accompanied by the following text: "The final publication is available at link.springer.com".

Research paper thumbnail of Antimicrobial activity of Sesbania grandiflora flower polyphenol extracts on some pathogenic bacteria and growth stimulatory effect on the probiotic organism Lactobacillus acidophilus

Microbiological Research, 2012

Please cite this article in press as: China R, et al. Antimicrobial activity of Sesbania grandifl... more Please cite this article in press as: China R, et al. Antimicrobial activity of Sesbania grandiflora flower polyphenol extracts on some pathogenic bacteria and growth stimulatory effect on the probiotic organism Lactobacillus acidophilus. Microbiol Res (2012), http://dx.

Research paper thumbnail of Comparative real-time study of cellular uptake of a formulated conjugated linolenic acid rich nano and conventional macro emulsions and their bioactivity in ex vivo models for parenteral applications

Colloids and surfaces. B, Biointerfaces, Jan 4, 2015

The objective of the present study was to fabricate and monitor real-time, impact of a stable con... more The objective of the present study was to fabricate and monitor real-time, impact of a stable conjugated linolenic acid, α-eleostearic acid (ESA) rich nanoemulsion (NE) formulation (d<200nm) vis-à-vis ESA conventional emulsion (CE) system in ex vivo systems against both endogenous and exogenous reactive oxygen species (ROS). Accordingly, stable nanoemulsion formulation of ESA was engineered with the aid of bitter melon seed oil and non-toxic excipients. Morphology and particle size of the emulsion formulations were studied to validate stability. The real-time rapid uptake of the ESA NE and its increased prophylactic efficacy against induced endogenous and exogenous ROS in terms of cell viability and membrane integrity was evaluated flow-cytometrically and with fluorescence microscopic analysis of different primary cells. It was found that the fabricated non-toxic ESA NE had stable parameters (hydrodynamic mean diameter, particle size distribution and zeta potential) for over 12 w...

Research paper thumbnail of Induced Diarrhea in Suckling Mice

Journal of medicinal food

In this study, the extract of a green leafy vegetable Oxalis corniculata (Oxalidaceae) was evalua... more In this study, the extract of a green leafy vegetable Oxalis corniculata (Oxalidaceae) was evaluated for its in vitro antibacterial and in vivo anti colonizing effect against common intestinal pathogenic bacteria. Methanolic extract (80%) of Oxalis corniculata (Oxalidaceae) leaf contained a polyphenol content of 910 mg Gallic acid equivalent per gram of dry weight and the yield was 8%. The flavonoid content was 2.353 g quercetin equivalent per 100 g of the extract. In vitro studies indicated that the extract inhibited numerous pathogenic bacteria like Staphylococcus aureus (ATCC 25922), Escherichia coli (ATCC 25923), Shigella dysenteriae 1 (NT4907), Shigella flexneri 2a (2457T), Shigella boydii 4 (BCH612), and Shigella sonnie phase I (IDH00968). The minimum inhibitory concentration (MIC) against E. coli (ATCC 25923) was minimal (0.08 mg/mL), whereas MIC against S. flexneri 2a (2457T) was higher (0.13 mg/mL). A suckling mouse model was developed which involved challenging the mice in...

Research paper thumbnail of Comparative prophylactic effects of α- eleostearic acid rich nano and conventional emulsions in induced diabetic rats

This e-offprint is for personal use only and shall not be self-archived in electronic repositorie... more This e-offprint is for personal use only and shall not be self-archived in electronic repositories. If you wish to self-archive your article, please use the accepted manuscript version for posting on your own website. You may further deposit the accepted manuscript version in any repository, provided it is only made publicly available 12 months after official publication or later and provided acknowledgement is given to the original source of publication and a link is inserted to the published article on Springer's website. The link must be accompanied by the following text: "The final publication is available at link.springer.com".

Research paper thumbnail of Comparative real-time study of cellular uptake of a formulated conjugated linolenic acid rich nano and conventional macro emulsions and their bioactivity in ex vivo models for parenteral applications

The objective of the present study was to fabricate and monitor real-time, impact of a stable con... more The objective of the present study was to fabricate and monitor real-time, impact of a stable conjugated
linolenic acid, -eleostearic acid (ESA) rich nanoemulsion (NE) formulation (d < 200 nm) vis-à-vis ESA
conventional emulsion (CE) system in ex vivo systems against both endogenous and exogenous reactive
oxygen species (ROS). Accordingly, stable nanoemulsion formulation of ESA was engineered with
the aid of bitter melon seed oil and non-toxic excipients. Morphology and particle size of the emulsion
formulations were studied to validate the stability. The real-time rapid uptake of the ESA NE and its
increased prophylactic efficacy against induced endogenous and exogenous ROS in terms of cell viability
and membrane integrity was evaluated flow-cytometrically and with fluorescence microscopic analysis
of different primary cells.
It was found that the fabricated non-toxic ESA NE had stable parameters (hydrodynamic mean diameter,
particle size distribution and zeta potential) for over 12 weeks. Further, ESA NE at a concentration of
∼70 M exhibited maximum efficacy in protecting cells from oxidative damage against both endogenous
and exogenous ROS in lymphocytes and hepatocytes as compared to its corresponding presence in the
CE formulation.
This study provides a real-time empirical evidence on the influence of nano formulation in enhancing
bioavailability and antioxidative properties of ESA.

Research paper thumbnail of Oxalis corniculata (Oxalidaceae) Leaf Extract Exerts In Vitro Antimicrobial and In Vivo Anticolonizing Activities Against Shigella dysenteriae 1 (NT4907) and Shigella flexneri 2a (2457T) in Induced Diarrhea in Suckling Mice

Journal of Medicinal Food, 2013

In this study, the extract of a green leafy vegetable Oxalis corniculata (Oxalidaceae) was evalua... more In this study, the extract of a green leafy vegetable Oxalis corniculata (Oxalidaceae) was evaluated for its in vitro antibacterial and in vivo anti colonizing effect against common intestinal pathogenic bacteria. Methanolic extract (80%) of Oxalis corniculata (Oxalidaceae) leaf contained a polyphenol content of 910 mg Gallic acid equivalent per gram of dry weight and the yield was 8%. The flavonoid content was 2.353 g quercetin equivalent per 100 g of the extract. In vitro studies indicated that the extract inhibited numerous pathogenic bacteria like Staphylococcus aureus (ATCC 25922), Escherichia coli (ATCC 25923), Shigella dysenteriae 1 (NT4907), Shigella flexneri 2a (2457T), Shigella boydii 4 (BCH612), and Shigella sonnie phase I (IDH00968). The minimum inhibitory concentration (MIC) against E. coli (ATCC 25923) was minimal (0.08 mg/mL), whereas MIC against S. flexneri 2a (2457T) was higher (0.13 mg/mL). A suckling mouse model was developed which involved challenging the mice intragastrically with S. flexneri 2a (2457T) and S. dysenteriae 1 (NT4907) to study the anticolonization activity. It was revealed that the extract was more potent against S. dysenteriae 1 (NT4907) as compared to S. flexneri 2a (2457T). It was also found that simultaneous administration of extract along with bacterial inoculums promoted good anticolonization activity. Significant activity was observed even when treated after 3 h of bacterial inoculation.

Research paper thumbnail of Evaluation of antioxidative, antibacterial and probiotic growth stimulatory activities of Sesamum indicum honey containing phenolic compounds and lignans

LWT - Food Science and Technology, 2015

Seven Sesamum indicum (sesame) honey samples were collected from Hooghly district of West Bengal,... more Seven Sesamum indicum (sesame) honey samples were collected from Hooghly district of West Bengal, India and analyzed for polyphenol and flavonoid content along with their in vitro free radical scavenging activities. Antibacterial activity and stimulatory effect on multiplication of probiotic bacteria were evaluated. Antioxidant markers like IC 50 value for DPPH (1, 1-diphenyl-2-picrylhydrazyl) and FRAP (ferric reducing antioxidant power) of sesame honey were positively correlated to its polyphenolic content (28.9 ± 0.6 mg GAE/100 g) and color intensity (r ranges between 0.872 and 0.931). Four flavonoids viz., apigenin, quercetin, myricetin, rutin have been identified along with one cinnamic acid derivative (ferulic acid) and two sesame lignans (sesamin and episesamin) by High performance liquid chromatography which can be used as a tool for authentication of sesame honey. Antibacterial activity of sesame honey against some enteropathogenic bacteria Escherichia coli, Vibrio cholerae, S. Typhi, S. Typhimurium were studied. Minimum inhibitory concentration was found to be lowest against S. Typhi (12.5% w/v) and S. Typhimurium (12.5% w/v). Plasmid DNA degradation by sesame honey administration evinced its molecular level of action. Interestingly, it was also found that sesame honey exhibited significant growth promoting property of probiotic strains such as Lactobacillus acidophilus and Bifidobacterium bifidum.

Research paper thumbnail of Comparative prophylactic effects of α-eleostearic acid rich nano and conventional emulsions in induced diabetic rats

Journal of Food Science and Technology, 2014

This e-offprint is for personal use only and shall not be self-archived in electronic repositorie... more This e-offprint is for personal use only and shall not be self-archived in electronic repositories. If you wish to self-archive your article, please use the accepted manuscript version for posting on your own website. You may further deposit the accepted manuscript version in any repository, provided it is only made publicly available 12 months after official publication or later and provided acknowledgement is given to the original source of publication and a link is inserted to the published article on Springer's website. The link must be accompanied by the following text: "The final publication is available at link.springer.com".

Research paper thumbnail of Antimicrobial activity of Sesbania grandiflora flower polyphenol extracts on some pathogenic bacteria and growth stimulatory effect on the probiotic organism Lactobacillus acidophilus

Microbiological Research, 2012

Please cite this article in press as: China R, et al. Antimicrobial activity of Sesbania grandifl... more Please cite this article in press as: China R, et al. Antimicrobial activity of Sesbania grandiflora flower polyphenol extracts on some pathogenic bacteria and growth stimulatory effect on the probiotic organism Lactobacillus acidophilus. Microbiol Res (2012), http://dx.

Research paper thumbnail of Comparative real-time study of cellular uptake of a formulated conjugated linolenic acid rich nano and conventional macro emulsions and their bioactivity in ex vivo models for parenteral applications

Colloids and surfaces. B, Biointerfaces, Jan 4, 2015

The objective of the present study was to fabricate and monitor real-time, impact of a stable con... more The objective of the present study was to fabricate and monitor real-time, impact of a stable conjugated linolenic acid, α-eleostearic acid (ESA) rich nanoemulsion (NE) formulation (d<200nm) vis-à-vis ESA conventional emulsion (CE) system in ex vivo systems against both endogenous and exogenous reactive oxygen species (ROS). Accordingly, stable nanoemulsion formulation of ESA was engineered with the aid of bitter melon seed oil and non-toxic excipients. Morphology and particle size of the emulsion formulations were studied to validate stability. The real-time rapid uptake of the ESA NE and its increased prophylactic efficacy against induced endogenous and exogenous ROS in terms of cell viability and membrane integrity was evaluated flow-cytometrically and with fluorescence microscopic analysis of different primary cells. It was found that the fabricated non-toxic ESA NE had stable parameters (hydrodynamic mean diameter, particle size distribution and zeta potential) for over 12 w...