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Papers by NURTEN ÖZSOY

Kumquat and calamondin are two small-size citrus fruits. Owing to their health benefits, they are... more Kumquat and calamondin are two small-size citrus fruits. Owing to their health benefits, they are traditionally used as folk medicine in Asian countries. However, the research on flavonoids and biological activities of kumquat and calamondin have received less attention. This review summarizes the reported quantitative and qualitative data of phenolic compositions in these two fruits. Effects of maturity, harvest time, various solvent extractions and heat treatment of phenolic compositions, and bioactivities were discussed; distributions of the forms of phenolic compounds existing in kumquat and calamondin were also summarized. Furthermore, biological activities, including antioxidant, antityrosinase, antimicrobial, antitumor, and antimetabolic disorder effects, have also been discussed. Effective phenolic components were proposed for a certain bioactivity. It was found that C-glycoside flavonoids are dominant phenolic compounds in kumquat and calamondin, unlike in other citrus fruits. Up to now, biological activities and chemical characteristics of C-glycoside flavonoids in kumquat and calamondin are largely unknown.

Ethyl acetate, methanol, dichloromethane, petroleum ether and water extracts of nine selected pla... more Ethyl acetate, methanol, dichloromethane, petroleum ether and water extracts of nine selected plant species, which are commonly used as herbal medicines for anticancer and anti-inflammatory purposes in Turkey, were evaluated for their total phenolic and flavonoid contents and in vitro antioxidant potency with a thiobarbituric acid assay using the lipid peroxidation of phosphatidylcholine liposomes, DPPH and ferric ion reducing antioxidant power assays. Inhibitory activity against cyclooxygenase (COX) was used to evaluate the anti-inflammatory activities of the extracts. As thioredoxin reductase (TrxR) has emerged as a new target for anticancer drug development, the extracts were investigated for their inhibitory activities on TrxR. The ability of the extracts to inhibit acetylcholinesterase (AChE), which is a target for cholinesterase inhibitors, used for the symptomatic treatment of Alzheimer's disease, was also examined. The results showed that the extracts of C. coggygria and M. officinalis subsp. officinalis are the most effective hydrogen and electron donors and contained the highest amounts of phenolic compounds; thus, they can be considered the best antioxidants among the nine plants selected for the study. All the plants showed inhibitory effects against AChE, COX-1 and COX-2, therefore may be of potential therapeutic interest for the treatment of neurodegenerative and inflammatory disorders. It was found that M. officinalis subsp. officinalis, C. coggyria, S. aucuparia and P. major subsp. major have a strong inhibitory effect on TrxR by up to 99 %, highlighting their potential as preventive therapeutics for cancer. This study confirmed the use of these plants in folk medicine as anti-inflammatory and anticancer agents.

Natural Product Research, Apr 10, 2010

Planta medica international open, Oct 1, 2017

South African Journal of Botany, Sep 1, 2019

Scorzonera L. (Asteraceae) species are widespread and utilised as traditional medicine and edible... more Scorzonera L. (Asteraceae) species are widespread and utilised as traditional medicine and edible plant. A phytochemical study on ethyl acetate fraction obtained from the ethanol extract of Scorzonera hieraciifolia Hayek (an endemic species of Turkey) roots resulted in isolation of nine compounds; 5-O-feruloyl quinic acid methyl ester (1), 1,5-di-O-feruloylquinic acid (2), chlorogenic acid methyl ester (3), 3-O-caffeoylquinic acid methyl ester (4), 1,3-di-O-caffeoylquinic acid methyl ester (5), 3,5-di-O-caffeoylquinic acid methyl ester (6), 4,5-di-Ocaffeoylquinic acid methyl ester (7), caffeic acid (8), 3-(4'-Hydroxyphenyl)-2-propenoic acid (4″-carboxyl)-phenyl ester (9). Structures of the compounds were determined using spectral methods. All substances were isolated for the first time from this species and compound 9 is new for the family Asteraceae. In vitro antioxidant, antiinflammatory and antimicrobial activities of petroleum ether, chloroform, ethyl acetate and n-butanol fractions were evaluated. Ethyl acetate fraction showed the highest amount of total phenolic content and antioxidant capacity. No significant antimicrobial activity was determined against tested bacteria and fungi. COX inhibition test was employed for anti-inflammatory studies, all fractions showed low inhibitory effect on both COX-1 and COX-2 enzymes in comparison with indomethacin.

Istanbul university journal of pharmacy, Oct 11, 2019

INTRODUCTION Thioredoxin (Trx), nicotinamide adenine dinucleotide phosphate (NADPH) and thioredox... more INTRODUCTION Thioredoxin (Trx), nicotinamide adenine dinucleotide phosphate (NADPH) and thioredoxin reductase (TrxR) comprise a thioredoxin system which exists in nearly all living cells (Arnér and Holmgren 2006). Trx, the physiological substrate for TrxR, occurs in either an oxidized or a reduced form. Reduced Trx prevents oxidation of various proteins by donating hydrogen atoms from two of the cysteine residues at its active site. Oxidized Trx is reduced by TrxR using NADPH as an electron donor (Seo and Lee 2010). TrxR belongs to the pyridine nucleotide-disulfide oxidoreductase family and is a dimeric flavoenzyme (Lu et al. 2009). The preliminary purification and characterization studies were performed with TrxR from archaea, bacteria and anaerobic amino-acid-utilizing bacteria (

Planta Medica, Jul 4, 2013

Planta Medica, Jul 1, 2012

Industrial Crops and Products, Oct 1, 2013

ABSTRACT The chemical composition of methanolic extract of Hypericum pamphylicum (HPM) was analyz... more ABSTRACT The chemical composition of methanolic extract of Hypericum pamphylicum (HPM) was analyzed by usinghigh performance liquid chromatography–diode array detector (HPLC-DAD). The plant contained hyper-icin (0.00002 ± 0.000001%), hyperforin (trace), chlorogenic acid (5.58 ± 1.59%), hyperoside (0.46 ± 0.05%),isoquercitrin (0.134 ± 0.02%) and quercetin (0.087 ± 0.008%). The antioxidant activity of HPM was eval-uated by using several antioxidant tests. HPM exhibits inhibitory capacity against phosphatidylcholineliposome peroxidation, induced with iron and ascorbic acid, scavenge DPPH and superoxide radicals andact as reductants. The cytotoxic activity of HPM was also determined by using MTT cell viability assay onHeLa and NRK-52E cell lines. HPM demonstrated no cytotoxicity against HeLa and NRK-52E cell lines withIC50values of 1.89 mg/ml and 1.05 mg/ml, respectively. The different crude extracts of H. pamphylicumwere analyzed using a microdilution assay for antimicrobial activity against several microorganisms. Themost active extract was HPM which showed activity against Staphylococcus aureus. It is observed thatnearly all extracts showed activity against Staphylococcus epidermidis, Escherichia coli, Klebsiella pneumo-niae, Pseudomonas aeruginosa, Salmonella thypi, Shigella flexneri, Proteus mirabilis and Candida albicans.

Journal of Faculty Pharmacy of Istanbul University, 2012

SUMMARY A new and rapid affinity chromatography method based on cyanogen bromide (CNBr)-activated... more SUMMARY A new and rapid affinity chromatography method based on cyanogen bromide (CNBr)-activated Sepharose 4B bound-ovalbumin is presented for the purification of the main lectin present in Aloe vera (L.) Burm. fil. The lectin was purified 60 fold to apparent homogeneity in native polyacrylamide disc gel electrophoresis (PAGE) showing an apparent molecular weight of 45000 kDa. The fact that sodium dodecyl sulfate (SDS)-PAGE gave a subunit molecular weight of 14 400 kDa tends to propose that the lectin is composed of three subunits and thus is in agreement with Aloctin I previously partially purified and characterized by us. The lectin did not exhibit antioxidant effect as assessed by the DPPH· radical-scavenging assay. OZET Aloe vera (L.) Burm. fil. lektinin saflastirilmasi icin, yeni ve hizli bir kromatografi yontemi olan, ovalbumin bagli-siyanojen bromur (CNBr) ile aktive edilmis Sefaroz 4B, afinite kromatografisi tanitilmaktadir. Lektin, kromatografi sonucunda 60 kez saflastiril...

Diabetin lens antioksidan sisteminde hasara neden olarak, lensin opaklasmasina yol actigi bilinme... more Diabetin lens antioksidan sisteminde hasara neden olarak, lensin opaklasmasina yol actigi bilinmektedir. Bu calismada, tip il diabette olumlu etkisi gosterilmis olan Aloe yaprak ekstresinIn, sicanlarin lenslerinde diabetin neden oldugu oksidatif hasara etkisi incelenmistir .Tip II diabet, 2 gunluk Wistar sican yavrularina 100 mg/kg dozda streptozotosin injeksiyonu ile olusturuldu (nOSTZ-diabetik sicanlar). 2 ay sonra, diabetik olan sicanlar 4 gruba ayrildi ve bu gruplara, 15 gun sureyle, gunde bir kez, agizdan, asagidaki ekstreler verildi: 1. Kontrol: fosfatla tampon lanmis % 0.9 NaCl (PBS; 6 ml/kg). 2. Aloe yaprak pulpasi ekstresi (500 mg/kg). 3. Aloe yaprak jeli ekstresi (10 ml/kg), 4. Glibenklamid (1 mg/kg). 15. gun, eter anestezisi altinda kesilen sicanlarin lensleri ayrilarak izotonik tuzlu suda homojenize edildi. Oksidatif stres parametreleri olarak secilen glutatyon (GSH) ve lipid peroksidasyonu (LPO) duzeyleri, lens dokusunda spektrofo to metrik yontemlerle tayin edildi. Alo...

International Journal of Pharmacy and Pharmaceutical Sciences, 2016

Objective: The aim of this study, we based on protective and antioxidant efficiency of Hypericum ... more Objective: The aim of this study, we based on protective and antioxidant efficiency of Hypericum perforatum that shows a wide range of beneficial effect in vitro and in vivo . Methods: The in vitro antioxidant activity of the extract was assessed by using several antioxidant tests. The cytotoxic activity of Hypericum perforatum was also determined by using 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide viability assay on ordinary used cell lines. In vivo experiments in Swiss mice were determined by performing behavioral, memory tests and histological study. According to tests results, H. perforatum may be relevant to the treatment of cognitive disorders. Results: The results of chemical analysis showed a hight level of hyperforin and quercitin that had an important antioxidant activity proved in vitro with the 2, 2-diphenyl-1-picrylhydrazyl, Anti-lactoperoxidase and superoxide dismutases; this antioxidant activity was confirmed in vivo after the non-toxic results by ...

Endometrial cancer is the most common gynaecological malignant disease. Despite of some arguments... more Endometrial cancer is the most common gynaecological malignant disease. Despite of some arguments about the preference of herbal medicine to pharmaceutical drugs, because of their minimal side effects herbal medicine is proven to be effective as a single application and/or an adjuvant therapy. Rosa agretis Savi (R. agretis Savi) which belongs to Rosaceae family has been used traditionally to treat allergy, antiatopic dermatitis, and some inflammatory diseases, is commonly studied. Anti-oxidant activity is also reported. The present study was undertaken to investigate the in vitro anticancer activity of R. agrestis parts (leaves, flowers) at endometrium cancer. Extracts in a concentration of 1 µg/ml to 200 µg/ml from leaves and flowers were tested on human endometrium cell line named Ischikawa for 72 h. Cell proliferation (total cell number) and apoptotic index (flow cytometry) were evaluated for 72 h. All the results were statistically analyzed using the independent Student’s t-test...

Planta Medica, 2012

Purpose: To investigate the anti-proliferative and antioxidant potentials of four different solve... more Purpose: To investigate the anti-proliferative and antioxidant potentials of four different solvent extracts of Phoenix paludosa Roxb leaves. Methods: Four different solvent (hexane, chloroform, ethyl acetate and methanol) leaf extracts of the plant were tested for cytotoxicity against four cancer cells, viz, MCF-7 (oestrogen positive breast cancer cell line), MDA-MB-231 (triple negative breast cancer cell line), SK-BR-3 (breast adenocarcinoma) and ACHN (renal adenocarcinoma) as well as two normal cell lines, namely, HEK-293 (embryonic kidney cells) and MCF-10A (normal mammary epithelial cells)]. 2, 2-Diphenyl-1-picrylhydrazyl (DPPH) and 2, 29-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) free radical scavenging assays were used to evaluate the antioxidant activity of the crude extracts.

Journal of Faculty Pharmacy of Istanbul University, 2016

Abstract: Aloe vera, sometimes referred as a “miraculous” or “wonder” plant, has been used by man... more Abstract: Aloe vera, sometimes referred as a “miraculous” or “wonder” plant, has been used by mankind for centuries for the treatment of different disorders due to the inner gel of its succulent leaves. Medical usage and applications of the main species, namely Aloe vera (L.) Burm. f. are mainly attributed to immunomodulatory or antioxidant activities. Our work on A. vera has begun with the separation of anthraquinones and continued with the purification and characterization of the lectins. In another study, hypoglycemic effect of A. vera leaf extracts was assayed in vivo, followed by the effect of the extracts on several tissues in diabetic rats. A. vera has shown a significant prophylactic effect on Ehrlich ascites tumour cells when given before tumour inoculation in mice. This effect was also seen with the purified lectin and attributed to the immunomodulatory effect of the plant. Assuming that its benefit could also be attributed to the antioxidant activity, the antioxidant pote...

Future Medicinal Chemistry

Background: Possible bioisosteres can be developed by replacing the 1-indanone ring (one of three... more Background: Possible bioisosteres can be developed by replacing the 1-indanone ring (one of three pharmacophore groups) of donepezil with an indoline ring. As H2S donors, thioamide, thiocarbamate and thiourea groups are also critically important. Materials & methods: The 1-benzyl-2-indolinones 6a–n were designed using molecular modeling and synthesized, and their acetylcholinesterase and butyrylcholinesterase inhibitory effects were then investigated. Results: The compounds 6h (inhibition constant [ Ki] = 0.22 μM; selectivity index [SI] = 26.22), 6i ( Ki = 0.24 μM; SI = 25.83), 6k ( Ki = 0.22 μM; SI = 28.31) and 6n ( Ki = 0.21 μM; SI = 27.14) were approximately twofold more effective against and >12-fold more selective for acetylcholinesterase compared with donepezil ( Ki = 0.41 μM; SI = 2.12). Analysis of molecular dynamics simulations with compounds 6k and 6n indicated that the preferred binding might be at allosteric binding pocket 4 of the enzyme. Conclusion: Benzyl substitut...

Figure S1-S54 Figure G1-G40

Marmara Pharmaceutical Journal, 2017

In this work, we report the synthesis, structural characterization and evaluation of in vitro ant... more In this work, we report the synthesis, structural characterization and evaluation of in vitro antioxidant and cytotoxic properties of novel spirobenzothiazolines (1a-e, 2a-e). 5-nonsubstituted spirobenzothiazolines (1a-e) demonstrated notable inhibitory capacity on lipid peroxidation (LPO), reducing power and scavinging effects on diphenylpicryl hydrazine (DPPH˙) and 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid (ABTS˙+) radicals, that were similar to that of α-tocopherol. The most potent antioxidant was compound 1a (incorporating an ethyl group on the cyclohexane ring) with an anti-LPO activity 2-fold higher than that of α-tocopherol. Compound 1a exhibited anti-LPO and DPPH˙ scavenging activities at concentrations lower than those cytotoxic for mouse normal fibroblast (NIH/3T3) cells and was also found to be slightly more selective for cancer cells (human prostat adenocarcinoma cell/PC-3) than normal mammalian cells.