Nagwan Sabek - Academia.edu (original) (raw)

Papers by Nagwan Sabek

American journal of biomedical research, Aug 27, 2016

Background: Hyperhomocysteinemia and platelet glycoprotein GpIIIa polymorphism had been identifie... more Background: Hyperhomocysteinemia and platelet glycoprotein GpIIIa polymorphism had been identified as risk factors for coronary atherosclerosis. The methylenetetrahydrofolate reductase MTHFR C677T variant has been shown to influence homocysteine metabolism, the interaction of plasma tHcy with other conventional risk factors remain uncertain in the clinical setting of acute myocardial infarction (AMI). The present study aimed to examine whether the MTHFR and platelet glycoprotein IIIa polymorphisms were associated with increased risk of (MI) in Egyptian patients. Subjects and Method: 150 newly diagnosed MI patients and 50 healthy matched subjects were recruited into this study, genotyping of the MTHFR C677T and GpIIIa 1565 A 1 /A 2 polymorphisms were carried out by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique, plasma tHcy, and folic acid levels were estimated. Results: Fasting plasma total Hcy levels were significantly higher in MI patients than controls (P <0.05), folate levels were significantly lower in MI patients than controls (P <0.05), no significant differences were observed in the MTHFR C677T and GpIIIa genotypes frequencies between MI patients and controls (P > 0.05). The frequency of the MTHFR C allele was 80.6 % and 76 % in MI patients and controls respectively and did not differ significantly between the two groups (P > 0.05). The frequency of risk allele, GpIIIa, PIA 2 was significantly higher in MI patients compared to controls (p<0.05), plasma tHcy level was significantly higher and folate level was significantly lower in MI patients carrying MTHFR CC and GPIIIa PIA 2 A 2 genotypes. Conclusions: In this population, the both risk alleles of MTHFR and GpIIb/IIIa polymorphisms had no major effect on the MI incidence, they were associated with higher homocysteine levels. A gene-environment interaction might increase the risk indirectly by elevating tHcy, especially when folate intake was low, our findings might support that MTHFR and GpIIb/IIIa polymorphisms as risk factors for MI.

Suez Canal University Medical Journal

Suez Canal University Medical Journal (Print), Oct 1, 2014

Background: Runx2 and Osterix are the key transcription factors initiating and regulating the ear... more Background: Runx2 and Osterix are the key transcription factors initiating and regulating the early osteogenesis and late mineralization of bone. Regenerative medicine is an evolving field of medical researches; this field holds the promise of regenerating damaged tissues and organs in the body by replacing damaged tissue and by stimulating the body's own repair mechanisms to heal previously irreparable tissues or organs. Aim: to investigate the osteogenic differentiation potential of mesenchymal stem cells isolated from human bone marrow (BM) and umbilical cord blood (UCB). Methods: Mononuclear cells isolated from human UCB and BM were differentiated into spindle shaped fibrous mesenchymal stem cells (MSCs) in Dulbecco modified Eagle's medium (DMEM) with 10% fetal bovine serum. These progenitor cells were further examined for their ability to differentiate into osteoblasts by culture in osteogenic differentiation media containing; dexamethazone and ascorbate-2phosphate and by the detection set of osteogenic genes expression including; Runx-2 and Osterix, and the markers of osteogenic genes differentiation: Collagen1 and alkaline phosphatase (ALP). Results: Runx-2 gene was significantly expressed about 7 fold in BM derived cells treated for 24 hours compared to control sample (which was not treated by osteogenic differentiation media) (7.2±1.1 vs. 1.0±0.1, p=0.02). In addition, there was significantly expression about 3 to 4 folds in UCB compared to control sample (3.4±0.4 vs. 0.6±0.1, p= 0.04). We also found that Osterix gene expression was significantly increased in BM MSCs treated samples for 24 hours about 3 folds relative to control (3.2±0.4 vs. 1.0±0.14, p= 0.02) and increased in UCB-MSCs samples treated for 24-72 hours about 2-4 folds respectively compared to control sample (2.1±0.5 vs. 0.7±0.09, p= 0.07). Conclusions: we conclude that i) UCBMSCs are capable of mineralization when cultured in osteogenic medium and would be a better cell source for treatment of osteogenic repair. ii) A significant change in osteogenic genes expression was found between both sources of mesenchymal stem cells indicating that BM MSCs have a higher osteogenic differentiation potential than UCB MSCs although UCB MSCs had proven to be an alternative source for mesenchymal stem cells and these cells are successfully differentiated into osteoblasts.

Menoufia Medical Journal, 2015

Objective This work aimed to study the effect of low intensity electrical stimulation and Mesench... more Objective This work aimed to study the effect of low intensity electrical stimulation and Mesenchymal stem cells transplantation on repair of sciatic nerve crush injury. Background Injury of peripheral nerves results in temporary or life-long neuronal dysfunction that can subsequently lead to economic or social disability. Despite early diagnosis and use of modern surgical techniques, functional recovery can never reach the pre-injury level. Several alternate approaches have been proposed to get beneficial effects on peripheral nerve regeneration, including application of electric field, transplantation of stem cells, and administration of neurotrophic factors. Materials and Methods 48 albino rats weighing 180:250 gm were used in this study. Rats were divided into four equal groups (12 rats each): Sham surgery group: sciatic nerve was exposed but not crushed. Injured sciatic nerve control group: sciatic nerve was exposed and crushed. Mesenchymal stem cells (MSCs) transplantation gro...

Menoufia Medical Journal, 2015

Objective This work aimed to study the effect of low intensity electrical stimulation and Mesench... more Objective This work aimed to study the effect of low intensity electrical stimulation and Mesenchymal stem cells transplantation on repair of sciatic nerve crush injury. Background Injury of peripheral nerves results in temporary or life-long neuronal dysfunction that can subsequently lead to economic or social disability. Despite early diagnosis and use of modern surgical techniques, functional recovery can never reach the pre-injury level. Several alternate approaches have been proposed to get beneficial effects on peripheral nerve regeneration, including application of electric field, transplantation of stem cells, and administration of neurotrophic factors. Materials and Methods 48 albino rats weighing 180:250 gm were used in this study. Rats were divided into four equal groups (12 rats each): Sham surgery group: sciatic nerve was exposed but not crushed. Injured sciatic nerve control group: sciatic nerve was exposed and crushed. Mesenchymal stem cells (MSCs) transplantation gro...

Background: Vitiligo is a common skin disease characterized by cutaneous white macules. The exact... more Background: Vitiligo is a common skin disease characterized by cutaneous white macules. The exact pathogenesis of vitiligo is not yet known. Estrogen receptor β (ERβ) was expressed in the melanocytes and has a possible role in pathogenesis of autoimmune diseases. Objective: This study aimed to assess serum estrogen and ERβ levels in female and male vitiligo patients and studied their association with the disease activity and severity; to our knowledge this study is the first one that assessed the serum level of estrogen and estrogen receptor β in patients with vitiligo. Subjects and Method: the study was conducted on 30 female and 30 male patients with vitiligo and 30 female and 30 male healthy controls. All patients were subjected to full history taking and clinical assessment; serum estrogen and ERβ were measured using enzyme linked immune-assay (ELISA) kits. Results: Our results showed that serum estrogen level was statistically significant higher in female vitiligo patients comp...

ABSTRACT Thrombospondin (TSP) 2 and 4 are multidomain calcium-binding extracellular glycoproteins... more ABSTRACT Thrombospondin (TSP) 2 and 4 are multidomain calcium-binding extracellular glycoproteins which play a role in platelet aggregation and inflammatory response. TSP-2 has chemotactic and mitogenic activities for vascular smooth muscle cells while TSP-4 mRNA is expressed by endothelial and smooth muscle cells in vascular wall, and brain endothelial cells produce the protein both in vivo and in cell culture, localization consistent with its pro-atherogenic effects. These common functions may be central to the roles of the thrombospondins in coronary artery disease and myocardial infarction (MI). In the present study, the association of the TSP-2 (3949 T→G, rs8089) and TSP-4 (Ala387Pro 1186 G→C, rs1866389) gene variations and MI among Egyptian patients living in Ismailia city has been examined. Both rs8089 and rs1866389 were studied in 50 acute MI patients and 50 controls using Real-Time polymerase chain reaction. The prevalence of TSP-2 and TSP-4 alleles was not different in MI ...

Background: Hyperhomocysteinemia and platelet glycoprotein GpIIIa polymorphism had been identifie... more Background: Hyperhomocysteinemia and platelet glycoprotein GpIIIa polymorphism had been identified as risk factors for coronary atherosclerosis. The methylenetetrahydrofolate reductase MTHFR C677T variant has been shown to influence homocysteine metabolism, the interaction of plasma tHcy with other conventional risk factors remain uncertain in the clinical setting of acute myocardial infarction (AMI). The present study aimed to examine whether the MTHFR and platelet glycoprotein IIIa polymorphisms were associated with increased risk of (MI) in Egyptian patients. Subjects and Method: 150 newly diagnosed MI patients and 50 healthy matched subjects were recruited into this study, genotyping of the MTHFR C677T and GpIIIa 1565 A1/A2 polymorphisms were carried out by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique, plasma tHcy, and folic acid levels were estimated. Results: Fasting plasma total Hcy levels were significantly higher in MI patients tha...

Introduction: Rampant caries (RC) is a severe form of dental decay with multifactorial origin. RC... more Introduction: Rampant caries (RC) is a severe form of dental decay with multifactorial origin. RC poses a challenging management problem for dentists and patients. Aim: the aim of the study was to evaluate and compare between some salivary parameters: pH, buffering capacity, total protein, flow rate and zinc concentration in a group of children suffering from rampant caries and others with normal caries activity in Ismailia city. Material and methods: sixty apparently healthy children aged from 3-5 years of both sexes. According to dmfs index the selected children were divided into three groups. Saliva samples were examined for salivary pH changes, measured by pH meter, Buffering capacity by titratable acidity, Total protein by colorimetric method, Flow rate by drooling method and Zinc concentration using an atomic absorption spectrophotometer. Results: there was no statistically significant difference between mean salivary pH values in three groups. Salivary buffering capacity resu...

Background: Vitiligo is a common skin disease characterized by cutaneous white macules. The exact... more Background: Vitiligo is a common skin disease characterized by cutaneous white macules. The exact pathogenesis of vitiligo is not yet known. Estrogen receptor β (ERβ) was expressed in the melanocytes and has a possible role in pathogenesis of autoimmune diseases. Objective: This study aimed to assess serum estrogen and ERβ levels in female and male vitiligo patients and studied their association with the disease activity and severity; to our knowledge this study is the first one that assessed the serum level of estrogen and estrogen receptor β in patients with vitiligo. Subjects and Method: the study was conducted on 30 female and 30 male patients with vitiligo and 30 female and 30 male healthy controls. All patients were subjected to full history taking and clinical assessment; serum estrogen and ERβ were measured using enzyme linked immune-assay (ELISA) kits. Results: Our results showed that serum estrogen level was statistically significant higher in female vitiligo patients comp...

Suez Canal University Medical Journal

Background: Runx2 and Osterix are the key transcription factors initiating and regulating the ear... more Background: Runx2 and Osterix are the key transcription factors initiating and regulating the early osteogenesis and late mineralization of bone. Regenerative medicine is an evolving field of medical researches; this field holds the promise of regenerating damaged tissues and organs in the body by replacing damaged tissue and by stimulating the body's own repair mechanisms to heal previously irreparable tissues or organs. Aim: to investigate the osteogenic differentiation potential of mesenchymal stem cells isolated from human bone marrow (BM) and umbilical cord blood (UCB). Methods: Mononuclear cells isolated from human UCB and BM were differentiated into spindle shaped fibrous mesenchymal stem cells (MSCs) in Dulbecco modified Eagle's medium (DMEM) with 10% fetal bovine serum. These progenitor cells were further examined for their ability to differentiate into osteoblasts by culture in osteogenic differentiation media containing; dexamethazone and ascorbate-2phosphate and by the detection set of osteogenic genes expression including; Runx-2 and Osterix, and the markers of osteogenic genes differentiation: Collagen1 and alkaline phosphatase (ALP). Results: Runx-2 gene was significantly expressed about 7 fold in BM derived cells treated for 24 hours compared to control sample (which was not treated by osteogenic differentiation media) (7.2±1.1 vs. 1.0±0.1, p=0.02). In addition, there was significantly expression about 3 to 4 folds in UCB compared to control sample (3.4±0.4 vs. 0.6±0.1, p= 0.04). We also found that Osterix gene expression was significantly increased in BM MSCs treated samples for 24 hours about 3 folds relative to control (3.2±0.4 vs. 1.0±0.14, p= 0.02) and increased in UCB-MSCs samples treated for 24-72 hours about 2-4 folds respectively compared to control sample (2.1±0.5 vs. 0.7±0.09, p= 0.07). Conclusions: we conclude that i) UCBMSCs are capable of mineralization when cultured in osteogenic medium and would be a better cell source for treatment of osteogenic repair. ii) A significant change in osteogenic genes expression was found between both sources of mesenchymal stem cells indicating that BM MSCs have a higher osteogenic differentiation potential than UCB MSCs although UCB MSCs had proven to be an alternative source for mesenchymal stem cells and these cells are successfully differentiated into osteoblasts.

Egyptian Journal of Ear, Nose, Throat and Allied Sciences

Background: genetic studies of laryngeal squamous cell carcinoma (LSCC) are rare in Egypt. Increa... more Background: genetic studies of laryngeal squamous cell carcinoma (LSCC) are rare in Egypt. Increased expression of some relevant genes may have diagnostic and therapeutic implications. Aim of the study: to compare the expression of HMGA2 and MMP12 genes in LSCC tumor tissue with their expression in adjacent non-tumor tissue. Type of the study: Descriptive study Setting: Tertiary referral hospital-Ismailia-Egypt Patients and Methods: 24 male patients (age range 49-78 years) with LSCC were included in the study. Under general anesthesia, two biopsies were taken: one from the tumor tissue and the other from the adjacent normal tissue and they were examined histopathologically and then analyzed for level of expression of MMP12 and HMGA2 genes. Results: Median expression levels of HMGA2 and MMP12 in the whole group were 0.98 and .8 (fold change) in normal tissue and 6.49 and 122.6 (fold change) in tumor tissue respectively. There was higher expression of the HMGA2 and MMP12 genes in tumor tissue compared to normal tissue (P <0.0001). Also there was positive correlation of level of expression of the two genes with the degree of tumor differentiation (P <.0001 and p<.0008) Conclusion: Expression of HMGA2 and MMP12 genes is higher in LSCC compared to normal tissue samples. The higher the level of expression of the two genes, the poorer the degree of differentiation of the tumor Further studies should be pursued to determine the diagnostic, therapeutic and prognostic implications of this in management of LSCC.

The Egyptian Journal of Biochemistry and Molecular Biology

Genome-wide association studies (GWAS) identified novel genes associated with type 2 diabetes mel... more Genome-wide association studies (GWAS) identified novel genes associated with type 2 diabetes mellitus (T2DM) which have been replicated in different ethnic populations and yielded inconsistent results. The insulin-like growth factor mRNA-2 binding protein 2 (IGF2BP2) is highly expressed in pancreatic islets, which play roles in normal embryonic growth and development. Our study aimed to replicate the association between insulin growth factor 2 mRNA binding protein 2 (IGF2BP2) gene (rs4402960) variant and T2DM in Egyptian diabetic patients resident in Ismailia city. The study included 152 subjects (76 unrelated T2DM patients and 76 control subjects) who were genotyped by polymerase chain reaction-restriction fragment length polymorphism technique (PCR-RFLP). Age, sex, blood pressure, BMI and Waist Circumference were recorded, and blood glucose, serum triglyceride, total cholesterol (TC), low-density lipoprotein-cholesterol (LDL-C), high-density lipoprotein-cholesterol (HDL-C) and homeostatic model assessment (HOMA) indices were determined. Fasting serum insulin and IGF2BP2 protein levels were analyzed by ELISA. For rs440960 variant of IGF2BP2 gene, risk allele T frequency was associated with T2DM [OR (95% CI)=1.82 (1.14-2.93), P= 0.012]. The frequency of T/T genotype versus (GG+G/T) genotypes was significantly higher in T2DM patients compared to controls (22.4% vs. 77.6% and 5.3% vs. 94.7%, respectively), (P=0.002). This association remained significant under additive (P=0.003) and co-dominant (P=0.009) genetic models. From this study, it could be concluded that IGF2BP2 rs4402960 polymorphism was found to be significantly associated with the Mohammed A. Hajar et all..

International journal of breast cancer, 2016

Epigenetic modifications are involved in breast carcinogenesis. Identifying genes that are epigen... more Epigenetic modifications are involved in breast carcinogenesis. Identifying genes that are epigenetically silenced via methylation could select target patients for diagnostic as well as therapeutic potential. We assessed promoter methylation of breast cancer susceptibility gene 1 (BRCA1) and 17 Beta Hydroxysteroid Dehydrogenase Type 1 (17βHSD-1) in normal and cancer breast tissues of forty sporadic breast cancer (BC) cases using restriction enzyme based methylation-specific PCR (REMS-PCR). In cancerous tissues, BRCA1 and 17βHSD-1 were methylated in 42.5% and 97.5%, respectively, while normal tissues had 35% and 95% methylation, respectively. BRCA1 methylation in normal tissues was 12.2-fold more likely to associate with methylation in cancer tissues (p < 0.001). It correlated significantly with increased age at menopause, mitosis, the negative status of Her2, and the molecular subtype "luminal A" (p = 0.048, p = 0.042, p = 0.007, and p = 0.049, resp.). Methylation of BR...

Journal of the Egyptian Society of Parasitology, 2007

The magnitude of Cyclospora oocysts excretion in relation to infection intensity among cyclospori... more The magnitude of Cyclospora oocysts excretion in relation to infection intensity among cyclosporiasis patients was assessed using flow cytometry and quantitative real-time PCR (RT-PCR). Oocysts from stool samples of 25 (14.8%) gastro-intestinal symptomatic pediatrics patients (169) and of 10 (2.8%) asymptomatic gastrointestinal ones (350) were identified by modified Ziehl-Neelsen (MZN) and modified Acid Fast Trichrome (MAFT) staining methods and confirmed by its auto-fluorescent characterizations. Also, 10 infants with negative stool samples were selected as controls. The intensity of infection was calculated as number of oocysts/200 microscopic filed with immersion 400. Flow cytometry and RT-PCR assessed relation between symptoms and oocysts excretions compared to MZN & MAFT. The infection severity in symptomatic patients were identified by MZN & MAFT as mild (16%), moderate (24%) and severe (60%) All asymptomatic patients had mild infection. Flow cytometry was done for stool sampl...

ABSTRACT Thrombospondin (TSP) 2 and 4 are multidomain calcium-binding extracellular glycoproteins... more ABSTRACT Thrombospondin (TSP) 2 and 4 are multidomain calcium-binding extracellular glycoproteins which play a role in platelet aggregation and inflammatory response. TSP-2 has chemotactic and mitogenic activities for vascular smooth muscle cells while TSP-4 mRNA is expressed by endothelial and smooth muscle cells in vascular wall, and brain endothelial cells produce the protein both in vivo and in cell culture, localization consistent with its pro-atherogenic effects. These common functions may be central to the roles of the thrombospondins in coronary artery disease and myocardial infarction (MI). In the present study, the association of the TSP-2 (3949 T→G, rs8089) and TSP-4 (Ala387Pro 1186 G→C, rs1866389) gene variations and MI among Egyptian patients living in Ismailia city has been examined. Both rs8089 and rs1866389 were studied in 50 acute MI patients and 50 controls using Real-Time polymerase chain reaction. The prevalence of TSP-2 and TSP-4 alleles was not different in MI ...

American journal of biomedical research, Aug 27, 2016

Background: Hyperhomocysteinemia and platelet glycoprotein GpIIIa polymorphism had been identifie... more Background: Hyperhomocysteinemia and platelet glycoprotein GpIIIa polymorphism had been identified as risk factors for coronary atherosclerosis. The methylenetetrahydrofolate reductase MTHFR C677T variant has been shown to influence homocysteine metabolism, the interaction of plasma tHcy with other conventional risk factors remain uncertain in the clinical setting of acute myocardial infarction (AMI). The present study aimed to examine whether the MTHFR and platelet glycoprotein IIIa polymorphisms were associated with increased risk of (MI) in Egyptian patients. Subjects and Method: 150 newly diagnosed MI patients and 50 healthy matched subjects were recruited into this study, genotyping of the MTHFR C677T and GpIIIa 1565 A 1 /A 2 polymorphisms were carried out by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique, plasma tHcy, and folic acid levels were estimated. Results: Fasting plasma total Hcy levels were significantly higher in MI patients than controls (P <0.05), folate levels were significantly lower in MI patients than controls (P <0.05), no significant differences were observed in the MTHFR C677T and GpIIIa genotypes frequencies between MI patients and controls (P > 0.05). The frequency of the MTHFR C allele was 80.6 % and 76 % in MI patients and controls respectively and did not differ significantly between the two groups (P > 0.05). The frequency of risk allele, GpIIIa, PIA 2 was significantly higher in MI patients compared to controls (p<0.05), plasma tHcy level was significantly higher and folate level was significantly lower in MI patients carrying MTHFR CC and GPIIIa PIA 2 A 2 genotypes. Conclusions: In this population, the both risk alleles of MTHFR and GpIIb/IIIa polymorphisms had no major effect on the MI incidence, they were associated with higher homocysteine levels. A gene-environment interaction might increase the risk indirectly by elevating tHcy, especially when folate intake was low, our findings might support that MTHFR and GpIIb/IIIa polymorphisms as risk factors for MI.

Suez Canal University Medical Journal

Suez Canal University Medical Journal (Print), Oct 1, 2014

Background: Runx2 and Osterix are the key transcription factors initiating and regulating the ear... more Background: Runx2 and Osterix are the key transcription factors initiating and regulating the early osteogenesis and late mineralization of bone. Regenerative medicine is an evolving field of medical researches; this field holds the promise of regenerating damaged tissues and organs in the body by replacing damaged tissue and by stimulating the body's own repair mechanisms to heal previously irreparable tissues or organs. Aim: to investigate the osteogenic differentiation potential of mesenchymal stem cells isolated from human bone marrow (BM) and umbilical cord blood (UCB). Methods: Mononuclear cells isolated from human UCB and BM were differentiated into spindle shaped fibrous mesenchymal stem cells (MSCs) in Dulbecco modified Eagle's medium (DMEM) with 10% fetal bovine serum. These progenitor cells were further examined for their ability to differentiate into osteoblasts by culture in osteogenic differentiation media containing; dexamethazone and ascorbate-2phosphate and by the detection set of osteogenic genes expression including; Runx-2 and Osterix, and the markers of osteogenic genes differentiation: Collagen1 and alkaline phosphatase (ALP). Results: Runx-2 gene was significantly expressed about 7 fold in BM derived cells treated for 24 hours compared to control sample (which was not treated by osteogenic differentiation media) (7.2±1.1 vs. 1.0±0.1, p=0.02). In addition, there was significantly expression about 3 to 4 folds in UCB compared to control sample (3.4±0.4 vs. 0.6±0.1, p= 0.04). We also found that Osterix gene expression was significantly increased in BM MSCs treated samples for 24 hours about 3 folds relative to control (3.2±0.4 vs. 1.0±0.14, p= 0.02) and increased in UCB-MSCs samples treated for 24-72 hours about 2-4 folds respectively compared to control sample (2.1±0.5 vs. 0.7±0.09, p= 0.07). Conclusions: we conclude that i) UCBMSCs are capable of mineralization when cultured in osteogenic medium and would be a better cell source for treatment of osteogenic repair. ii) A significant change in osteogenic genes expression was found between both sources of mesenchymal stem cells indicating that BM MSCs have a higher osteogenic differentiation potential than UCB MSCs although UCB MSCs had proven to be an alternative source for mesenchymal stem cells and these cells are successfully differentiated into osteoblasts.

Menoufia Medical Journal, 2015

Objective This work aimed to study the effect of low intensity electrical stimulation and Mesench... more Objective This work aimed to study the effect of low intensity electrical stimulation and Mesenchymal stem cells transplantation on repair of sciatic nerve crush injury. Background Injury of peripheral nerves results in temporary or life-long neuronal dysfunction that can subsequently lead to economic or social disability. Despite early diagnosis and use of modern surgical techniques, functional recovery can never reach the pre-injury level. Several alternate approaches have been proposed to get beneficial effects on peripheral nerve regeneration, including application of electric field, transplantation of stem cells, and administration of neurotrophic factors. Materials and Methods 48 albino rats weighing 180:250 gm were used in this study. Rats were divided into four equal groups (12 rats each): Sham surgery group: sciatic nerve was exposed but not crushed. Injured sciatic nerve control group: sciatic nerve was exposed and crushed. Mesenchymal stem cells (MSCs) transplantation gro...

Menoufia Medical Journal, 2015

Objective This work aimed to study the effect of low intensity electrical stimulation and Mesench... more Objective This work aimed to study the effect of low intensity electrical stimulation and Mesenchymal stem cells transplantation on repair of sciatic nerve crush injury. Background Injury of peripheral nerves results in temporary or life-long neuronal dysfunction that can subsequently lead to economic or social disability. Despite early diagnosis and use of modern surgical techniques, functional recovery can never reach the pre-injury level. Several alternate approaches have been proposed to get beneficial effects on peripheral nerve regeneration, including application of electric field, transplantation of stem cells, and administration of neurotrophic factors. Materials and Methods 48 albino rats weighing 180:250 gm were used in this study. Rats were divided into four equal groups (12 rats each): Sham surgery group: sciatic nerve was exposed but not crushed. Injured sciatic nerve control group: sciatic nerve was exposed and crushed. Mesenchymal stem cells (MSCs) transplantation gro...

Background: Vitiligo is a common skin disease characterized by cutaneous white macules. The exact... more Background: Vitiligo is a common skin disease characterized by cutaneous white macules. The exact pathogenesis of vitiligo is not yet known. Estrogen receptor β (ERβ) was expressed in the melanocytes and has a possible role in pathogenesis of autoimmune diseases. Objective: This study aimed to assess serum estrogen and ERβ levels in female and male vitiligo patients and studied their association with the disease activity and severity; to our knowledge this study is the first one that assessed the serum level of estrogen and estrogen receptor β in patients with vitiligo. Subjects and Method: the study was conducted on 30 female and 30 male patients with vitiligo and 30 female and 30 male healthy controls. All patients were subjected to full history taking and clinical assessment; serum estrogen and ERβ were measured using enzyme linked immune-assay (ELISA) kits. Results: Our results showed that serum estrogen level was statistically significant higher in female vitiligo patients comp...

ABSTRACT Thrombospondin (TSP) 2 and 4 are multidomain calcium-binding extracellular glycoproteins... more ABSTRACT Thrombospondin (TSP) 2 and 4 are multidomain calcium-binding extracellular glycoproteins which play a role in platelet aggregation and inflammatory response. TSP-2 has chemotactic and mitogenic activities for vascular smooth muscle cells while TSP-4 mRNA is expressed by endothelial and smooth muscle cells in vascular wall, and brain endothelial cells produce the protein both in vivo and in cell culture, localization consistent with its pro-atherogenic effects. These common functions may be central to the roles of the thrombospondins in coronary artery disease and myocardial infarction (MI). In the present study, the association of the TSP-2 (3949 T→G, rs8089) and TSP-4 (Ala387Pro 1186 G→C, rs1866389) gene variations and MI among Egyptian patients living in Ismailia city has been examined. Both rs8089 and rs1866389 were studied in 50 acute MI patients and 50 controls using Real-Time polymerase chain reaction. The prevalence of TSP-2 and TSP-4 alleles was not different in MI ...

Background: Hyperhomocysteinemia and platelet glycoprotein GpIIIa polymorphism had been identifie... more Background: Hyperhomocysteinemia and platelet glycoprotein GpIIIa polymorphism had been identified as risk factors for coronary atherosclerosis. The methylenetetrahydrofolate reductase MTHFR C677T variant has been shown to influence homocysteine metabolism, the interaction of plasma tHcy with other conventional risk factors remain uncertain in the clinical setting of acute myocardial infarction (AMI). The present study aimed to examine whether the MTHFR and platelet glycoprotein IIIa polymorphisms were associated with increased risk of (MI) in Egyptian patients. Subjects and Method: 150 newly diagnosed MI patients and 50 healthy matched subjects were recruited into this study, genotyping of the MTHFR C677T and GpIIIa 1565 A1/A2 polymorphisms were carried out by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique, plasma tHcy, and folic acid levels were estimated. Results: Fasting plasma total Hcy levels were significantly higher in MI patients tha...

Introduction: Rampant caries (RC) is a severe form of dental decay with multifactorial origin. RC... more Introduction: Rampant caries (RC) is a severe form of dental decay with multifactorial origin. RC poses a challenging management problem for dentists and patients. Aim: the aim of the study was to evaluate and compare between some salivary parameters: pH, buffering capacity, total protein, flow rate and zinc concentration in a group of children suffering from rampant caries and others with normal caries activity in Ismailia city. Material and methods: sixty apparently healthy children aged from 3-5 years of both sexes. According to dmfs index the selected children were divided into three groups. Saliva samples were examined for salivary pH changes, measured by pH meter, Buffering capacity by titratable acidity, Total protein by colorimetric method, Flow rate by drooling method and Zinc concentration using an atomic absorption spectrophotometer. Results: there was no statistically significant difference between mean salivary pH values in three groups. Salivary buffering capacity resu...

Background: Vitiligo is a common skin disease characterized by cutaneous white macules. The exact... more Background: Vitiligo is a common skin disease characterized by cutaneous white macules. The exact pathogenesis of vitiligo is not yet known. Estrogen receptor β (ERβ) was expressed in the melanocytes and has a possible role in pathogenesis of autoimmune diseases. Objective: This study aimed to assess serum estrogen and ERβ levels in female and male vitiligo patients and studied their association with the disease activity and severity; to our knowledge this study is the first one that assessed the serum level of estrogen and estrogen receptor β in patients with vitiligo. Subjects and Method: the study was conducted on 30 female and 30 male patients with vitiligo and 30 female and 30 male healthy controls. All patients were subjected to full history taking and clinical assessment; serum estrogen and ERβ were measured using enzyme linked immune-assay (ELISA) kits. Results: Our results showed that serum estrogen level was statistically significant higher in female vitiligo patients comp...

Suez Canal University Medical Journal

Background: Runx2 and Osterix are the key transcription factors initiating and regulating the ear... more Background: Runx2 and Osterix are the key transcription factors initiating and regulating the early osteogenesis and late mineralization of bone. Regenerative medicine is an evolving field of medical researches; this field holds the promise of regenerating damaged tissues and organs in the body by replacing damaged tissue and by stimulating the body's own repair mechanisms to heal previously irreparable tissues or organs. Aim: to investigate the osteogenic differentiation potential of mesenchymal stem cells isolated from human bone marrow (BM) and umbilical cord blood (UCB). Methods: Mononuclear cells isolated from human UCB and BM were differentiated into spindle shaped fibrous mesenchymal stem cells (MSCs) in Dulbecco modified Eagle's medium (DMEM) with 10% fetal bovine serum. These progenitor cells were further examined for their ability to differentiate into osteoblasts by culture in osteogenic differentiation media containing; dexamethazone and ascorbate-2phosphate and by the detection set of osteogenic genes expression including; Runx-2 and Osterix, and the markers of osteogenic genes differentiation: Collagen1 and alkaline phosphatase (ALP). Results: Runx-2 gene was significantly expressed about 7 fold in BM derived cells treated for 24 hours compared to control sample (which was not treated by osteogenic differentiation media) (7.2±1.1 vs. 1.0±0.1, p=0.02). In addition, there was significantly expression about 3 to 4 folds in UCB compared to control sample (3.4±0.4 vs. 0.6±0.1, p= 0.04). We also found that Osterix gene expression was significantly increased in BM MSCs treated samples for 24 hours about 3 folds relative to control (3.2±0.4 vs. 1.0±0.14, p= 0.02) and increased in UCB-MSCs samples treated for 24-72 hours about 2-4 folds respectively compared to control sample (2.1±0.5 vs. 0.7±0.09, p= 0.07). Conclusions: we conclude that i) UCBMSCs are capable of mineralization when cultured in osteogenic medium and would be a better cell source for treatment of osteogenic repair. ii) A significant change in osteogenic genes expression was found between both sources of mesenchymal stem cells indicating that BM MSCs have a higher osteogenic differentiation potential than UCB MSCs although UCB MSCs had proven to be an alternative source for mesenchymal stem cells and these cells are successfully differentiated into osteoblasts.

Egyptian Journal of Ear, Nose, Throat and Allied Sciences

Background: genetic studies of laryngeal squamous cell carcinoma (LSCC) are rare in Egypt. Increa... more Background: genetic studies of laryngeal squamous cell carcinoma (LSCC) are rare in Egypt. Increased expression of some relevant genes may have diagnostic and therapeutic implications. Aim of the study: to compare the expression of HMGA2 and MMP12 genes in LSCC tumor tissue with their expression in adjacent non-tumor tissue. Type of the study: Descriptive study Setting: Tertiary referral hospital-Ismailia-Egypt Patients and Methods: 24 male patients (age range 49-78 years) with LSCC were included in the study. Under general anesthesia, two biopsies were taken: one from the tumor tissue and the other from the adjacent normal tissue and they were examined histopathologically and then analyzed for level of expression of MMP12 and HMGA2 genes. Results: Median expression levels of HMGA2 and MMP12 in the whole group were 0.98 and .8 (fold change) in normal tissue and 6.49 and 122.6 (fold change) in tumor tissue respectively. There was higher expression of the HMGA2 and MMP12 genes in tumor tissue compared to normal tissue (P <0.0001). Also there was positive correlation of level of expression of the two genes with the degree of tumor differentiation (P <.0001 and p<.0008) Conclusion: Expression of HMGA2 and MMP12 genes is higher in LSCC compared to normal tissue samples. The higher the level of expression of the two genes, the poorer the degree of differentiation of the tumor Further studies should be pursued to determine the diagnostic, therapeutic and prognostic implications of this in management of LSCC.

The Egyptian Journal of Biochemistry and Molecular Biology

Genome-wide association studies (GWAS) identified novel genes associated with type 2 diabetes mel... more Genome-wide association studies (GWAS) identified novel genes associated with type 2 diabetes mellitus (T2DM) which have been replicated in different ethnic populations and yielded inconsistent results. The insulin-like growth factor mRNA-2 binding protein 2 (IGF2BP2) is highly expressed in pancreatic islets, which play roles in normal embryonic growth and development. Our study aimed to replicate the association between insulin growth factor 2 mRNA binding protein 2 (IGF2BP2) gene (rs4402960) variant and T2DM in Egyptian diabetic patients resident in Ismailia city. The study included 152 subjects (76 unrelated T2DM patients and 76 control subjects) who were genotyped by polymerase chain reaction-restriction fragment length polymorphism technique (PCR-RFLP). Age, sex, blood pressure, BMI and Waist Circumference were recorded, and blood glucose, serum triglyceride, total cholesterol (TC), low-density lipoprotein-cholesterol (LDL-C), high-density lipoprotein-cholesterol (HDL-C) and homeostatic model assessment (HOMA) indices were determined. Fasting serum insulin and IGF2BP2 protein levels were analyzed by ELISA. For rs440960 variant of IGF2BP2 gene, risk allele T frequency was associated with T2DM [OR (95% CI)=1.82 (1.14-2.93), P= 0.012]. The frequency of T/T genotype versus (GG+G/T) genotypes was significantly higher in T2DM patients compared to controls (22.4% vs. 77.6% and 5.3% vs. 94.7%, respectively), (P=0.002). This association remained significant under additive (P=0.003) and co-dominant (P=0.009) genetic models. From this study, it could be concluded that IGF2BP2 rs4402960 polymorphism was found to be significantly associated with the Mohammed A. Hajar et all..

International journal of breast cancer, 2016

Epigenetic modifications are involved in breast carcinogenesis. Identifying genes that are epigen... more Epigenetic modifications are involved in breast carcinogenesis. Identifying genes that are epigenetically silenced via methylation could select target patients for diagnostic as well as therapeutic potential. We assessed promoter methylation of breast cancer susceptibility gene 1 (BRCA1) and 17 Beta Hydroxysteroid Dehydrogenase Type 1 (17βHSD-1) in normal and cancer breast tissues of forty sporadic breast cancer (BC) cases using restriction enzyme based methylation-specific PCR (REMS-PCR). In cancerous tissues, BRCA1 and 17βHSD-1 were methylated in 42.5% and 97.5%, respectively, while normal tissues had 35% and 95% methylation, respectively. BRCA1 methylation in normal tissues was 12.2-fold more likely to associate with methylation in cancer tissues (p < 0.001). It correlated significantly with increased age at menopause, mitosis, the negative status of Her2, and the molecular subtype "luminal A" (p = 0.048, p = 0.042, p = 0.007, and p = 0.049, resp.). Methylation of BR...

Journal of the Egyptian Society of Parasitology, 2007

The magnitude of Cyclospora oocysts excretion in relation to infection intensity among cyclospori... more The magnitude of Cyclospora oocysts excretion in relation to infection intensity among cyclosporiasis patients was assessed using flow cytometry and quantitative real-time PCR (RT-PCR). Oocysts from stool samples of 25 (14.8%) gastro-intestinal symptomatic pediatrics patients (169) and of 10 (2.8%) asymptomatic gastrointestinal ones (350) were identified by modified Ziehl-Neelsen (MZN) and modified Acid Fast Trichrome (MAFT) staining methods and confirmed by its auto-fluorescent characterizations. Also, 10 infants with negative stool samples were selected as controls. The intensity of infection was calculated as number of oocysts/200 microscopic filed with immersion 400. Flow cytometry and RT-PCR assessed relation between symptoms and oocysts excretions compared to MZN & MAFT. The infection severity in symptomatic patients were identified by MZN & MAFT as mild (16%), moderate (24%) and severe (60%) All asymptomatic patients had mild infection. Flow cytometry was done for stool sampl...

ABSTRACT Thrombospondin (TSP) 2 and 4 are multidomain calcium-binding extracellular glycoproteins... more ABSTRACT Thrombospondin (TSP) 2 and 4 are multidomain calcium-binding extracellular glycoproteins which play a role in platelet aggregation and inflammatory response. TSP-2 has chemotactic and mitogenic activities for vascular smooth muscle cells while TSP-4 mRNA is expressed by endothelial and smooth muscle cells in vascular wall, and brain endothelial cells produce the protein both in vivo and in cell culture, localization consistent with its pro-atherogenic effects. These common functions may be central to the roles of the thrombospondins in coronary artery disease and myocardial infarction (MI). In the present study, the association of the TSP-2 (3949 T→G, rs8089) and TSP-4 (Ala387Pro 1186 G→C, rs1866389) gene variations and MI among Egyptian patients living in Ismailia city has been examined. Both rs8089 and rs1866389 were studied in 50 acute MI patients and 50 controls using Real-Time polymerase chain reaction. The prevalence of TSP-2 and TSP-4 alleles was not different in MI ...