Nilufar Mamadalieva - Academia.edu (original) (raw)

Papers by Nilufar Mamadalieva

Phytopharmacology, 2013

Ajuga turkestanica Rgl. Brig (Lamiaceae) is a medicinal plant from Uzbekistan. Methanol, chlorofo... more Ajuga turkestanica Rgl. Brig (Lamiaceae) is a medicinal plant from Uzbekistan. Methanol, chloroform, butanol, and water extracts as well as isolated phytoecdysteroids and iridoids were evaluated for their antioxidant, cytotoxic and antibacterial activities. Water and butanol extracts exhibited good antioxidant activity with IC50 values of 7.24±0.82 and 14.57±1.64 µg/mL. The chloroform extract showed potent cytotoxic effects against the cancer cell lines HeLa, HepG-2, and MCF-7 with IC50 values of 7.13±0.85, 9.03±0.92, and 10.77 ...

Chemistry of Natural Compounds, 2009

We have previously reported that 2-deoxyecdysone, 2-deoxy-20-hydroxyecdysone, polipodin B, 20-hyd... more We have previously reported that 2-deoxyecdysone, 2-deoxy-20-hydroxyecdysone, polipodin B, 20-hydroxyecdysone, 26-hydroxypolipodin B, integristerone A, 2,22-diacetate-and 3,22-diacetate-20,26-dihydroxyecdysone, sileneoside A, and sileneoside D were isolated from the aerial part of Silene viridiflora L. (Caryophyllaceae) introduced into the experimental plot of the ICPS AS RU [1,. In continuation of research on the chemical composition of this plant, we isolated fractions containing phytoecdysteroid 1 by chromatography of the BuOH extract over silica gel using CHCl 3 :CH 3 OH (9:1). Fractions were rechromatographed using CHCl 3 :CH 3 OH (15:1) to afford 1. The PMR and mass spectra allowed 1 to be identified as taxisterone, C 27 H 44 O 6 . Mass spectrum (CI, NH 3 , m/z): 465 [M + H] + ,

Natural Product Research, 2013

cenoic (oleic) acid (8.21%), stigmasterol (2.68%), b-sitosterol (2.65%) and 5,2 0dihydroxy-6,7,8,... more cenoic (oleic) acid (8.21%), stigmasterol (2.68%), b-sitosterol (2.65%) and 5,2 0dihydroxy-6,7,8,6 0 -tetramethoxyflavone (2.13%). In addition, 5,6-dihydroxy-7,8dimethoxyflavone was isolated from the same fraction by PTLC.

Methanol, butanol, chloroform and water extracts and individually six phytoecdysteroids (viticost... more Methanol, butanol, chloroform and water extracts and individually six phytoecdysteroids (viticosterone E, 20-hydroxyecdysone-22-benzoate, 2-deoxy-20-hydroxyecdysone, 2-deoxyecdysone, 20-hydroxyecdysone and integristerone A) isolated from a Silene wallichiana Klotsch. plant were evaluated for their antibacterial and antiproliferative properties. The methanolic extract inhibited the growth of Acinetobacter sp., Enterococcus faecalis, Klebsiella oxytoca, Pantoea agglomerans, Proteus rettgeri, Pseudomonas aeruginosa and Staphylococcus aureus at an MIC of 2.5 mg/ml, while Escherichia coli and Klebsiella pneumoniae were inhibited at an MIC of 1.25 mg/ml. The proliferation of cancer cells was potently inhibited by the chloroform extract (IC50 28.53 ± 1.98 µg/ml in HeLa, 26.34 ± 2.34 µg/ml in HepG-2 cells). Aqueous and butanol extracts exhibited good antioxidant activity with IC50 values of 24.83 ± 2.82 and 46.32 ± 3.73 µg/ml. Keywords: antibacterial, antioxidant, cytotoxic activity, phytoe...

Acta Horticulturae, 2014

Of the more than 4500 species of higher plants that grow wild in Uzbekistan, over 100 species wit... more Of the more than 4500 species of higher plants that grow wild in Uzbekistan, over 100 species within the Lamiaceae family are native to the country. The cytotoxicity of 10 of these plant species was investigated for antiproliferative properties using methanol, hexane, chloroform, and water extracts. All tested water extracts of the plants exhibited moderate inhibition of cell proliferation with IC 50 values above 200 g/ml. The chloroform and hexane extracts showed more potent inhibition of cell proliferation on tested cells than that of the methanol and water extracts. The chloroform extract of Ajuga turkestanica, Phlomis bucharica, and Scutellaria ramosissima strongly inhibited cell growth in HeLa cells with IC 50 values of 7.1, 26.3, and 23.2 g/ml, respectively. The higher levels of cytotoxic activity were observed in chloroform and hexane extracts of A. turkestanica, Leonurus turkestanicus, P. bucharica, Phlomis thapsoides, S. ramosissima, and Stachys betonicflora to HL-60 cells. Both chloroform and hexane extracts of L. turkestanicus, P. bucharica, S. ramosissima, and Thymus seravschanicus displayed very pronounced activity towards P3X cell lines. Proc. IS on Medicinal Plants and Natural Products Eds.: J. Ghaemghami et al. Acta Hort. 1030, ISHS 2014 62

Chemistry of Natural Compounds

$Research paper thumbnail of Lipid fraction from Scutellaria ramosissima tested on the microtubular array of cancer cell models$

Scutellaria ramosissima (Lamiaceae) is a plant used in traditional Uzbek medicine for cancer and ... more Scutellaria ramosissima (Lamiaceae) is a plant used in traditional Uzbek medicine for cancer and inflammatory therapy. The aim of this work is to investigate the microtubular component of the HeLa and HL-60 cells cytoskeleton after treatment with the isolated lipid fractions from S. ramosissima by using immunofluorescence analysis. In our hands a relation between the administration of isolated lipid fractions and the microtubule array did not occur. Our data confirmed that the microtubular cytoskeleton is not the cytoplasmic target of molecules consisting the lipid fractions from Scutellaria ramosissima.

Phytochemical analysis : PCA, Jan 8, 2015

Ajuga turkestanica is a plant used in traditional medicine for its high ecdysteroid content, incl... more Ajuga turkestanica is a plant used in traditional medicine for its high ecdysteroid content, including the presence of the particularly active turkesterone, which possess efficient anabolic activity. To isolate and identify minor ecdysteroids present in a semi-purified plant fraction containing ca. 70% turkesterone. Multi-step preparative HPLC (combining RP- and NP-HPLC systems) was used to purify the different components present in the turkesterone fraction. Isolated compounds were identified by high-resolution mass spectrometry and 2D-NMR. Fourteen ecdysteroids (including turkesterone and 20-hydroxyecdysone) were isolated. Seven of these, all bearing an 11α-hydroxy group, were previously unreported. Ajuga turkestanica ecdysteroids are characterised by the abundance of 11α-hydroxylated compounds and by the simultaneous presence of 24C, 27C, 28C and 29C ecdysteroids. It is expected that even more ecdysteroids are to be found in this plant since the starting material for this study l...

Natural product communications, 2010

Chemical investigations of Silene viridiflora (L.) yielded a new ecdysteroid, 20-hydroxyecdysone ... more Chemical investigations of Silene viridiflora (L.) yielded a new ecdysteroid, 20-hydroxyecdysone 20,22-monoacetonide-25-acetate (1), and a known ecdysteroid, 2-deoxypolypodine B-3-beta-D-glucoside (2). The elucidation of the chemical structures was established by 1D and 2D NMR experiments.

Natural product communications, 2013

Ethanolic extracts of Salvia officinalis leaves demonstrated antibacterial activity against Bacil... more Ethanolic extracts of Salvia officinalis leaves demonstrated antibacterial activity against Bacillus cereus. Fractionation of the extracts led to the isolation of the most active antibacterial compound, which, from spectroscopic and LC-MS evidence, was proved to be the diterpene, methyl carnosate.

The action of plant-derived 20-hydroxyecdysone (20E) on germination and seedling growth of three ... more The action of plant-derived 20-hydroxyecdysone (20E) on germination and seedling growth of three cotton varieties under various concentrations of NaCl and MgSO 4 were studied. Seed dormancy enforced by salinity (100 mM NaCl) was substantially alleviated and germination was significantly promoted by 20E from 31 to 39% in var. 'Namangan-77' and from 8 to 21% in 'AH-balut-2'. In contrast, seed germination of 'C-6524' variety was less affected by 20E. The 20E significantly stimulated seedling root and shoot growth of 'Namangan-77' and 'AH-balut-2' varieties at 10 -4 M NaCl or MgSO 4 . Our results showed that the application of 10 -4 M 20E improves cotton seed germination and protects seedlings from saline stress, although this effect is dependent upon cultivar.

Seven ecdysteroids isolated from the aerial part of Silene linicola are identified using H and C ... more Seven ecdysteroids isolated from the aerial part of Silene linicola are identified using H and C NMR 1 13 methods. Their yield from raw material was determined.