Noor Amirmozafari - Academia.edu (original) (raw)
Uploads
Papers by Noor Amirmozafari
Scientific Journal of Kurdistan University of Medical Sciences, Jun 15, 2006
Background: Staphylococcus aureus is one of the most important etiological agent of hospital and ... more Background: Staphylococcus aureus is one of the most important etiological agent of hospital and community acquired infections. The enterotoxins and toxin shock syndrom toxin (TSST-1) are among the most common pathogenic determinants elaborated by this bacterium. They are also well-known for their super-antigenic properties and are commonly reffered to as pyrogenic toxins super antigens (PTSAgs) which exert deep influences in their host. Infections caused by drug-resistant S. aureus strains, mostly of hospital origin, are rapidly on the rise in many parts of the world. The incidence of TSST-1 elaborating strains are also very alarming. The aim of this investigation was to survey the prevalence of TSST-1 gene in the clinical isolates of S. aureus recovered from hospitalized pateints in Shohada hospital of Tabriz, Iran. Methods: During one year period, a total of 11353 clinical specimens, obtained from hospitalized patients were subjected to bacterial culture. Strains of Staphylococcu...
The Journal of Genes, Microbes and Immunity, 2014
Multidrug resistance (MDR) Acinetobacter baumannii, producing the extended-spectrum β-lactamases ... more Multidrug resistance (MDR) Acinetobacter baumannii, producing the extended-spectrum β-lactamases (ESBL) is one of the major concerns in hospitals. According to the infection reports caused by A.baumannii in Iran, the aim of this study is to identify the genes encoding the β-lactamases TEM and SHV in A.baumannii isolates in Tehran hospitals using PCR method. A total number of 200 clinical isolates A. baumanniii were diagnosed at first using culture methods and biochemical tests followed by antibiogram using 16 different antibiotics by disk diffusion method based on CLSI guidelines. The ESBL was determined by double disk synergy test (DDST) and the results were verified using combined disk method. PCR for determining the prevalence of genes was performed using specific primers for all isolates. There was a high antibiotic resistance among the isolates with a high MDR rate of 82.5% and 51% strains were producing ESBL. The minimum inhibitory concentration (MIC) rate of cefepime and ceftazidime in the isolates were 8-128 μg/ml. The prevalence of TEM and SHV were respectively 56% and 63%. These results indicate the necessity of a monitoring program to timely identify these strains to prevent the resistance spread and increase efficient antibiotics usage.
Journal of Periodontal Research, 1996
Culture and the polymerase chain reaction (PCR) were compared for detection of Borrelia burgdorfe... more Culture and the polymerase chain reaction (PCR) were compared for detection of Borrelia burgdorferi infection in wild-caught Peromyscus leucopus and experimentally inoculated C.B-17 scid/scid (severe combined immunodeficient) mice. PCR targeted highly conserved regions of the ospA gene and could detect one to five cultured organisms and 10 to 50 copies of molecularly cloned ospA DNA. Organs (kidney, spleen, and urinary bladder) and/or ear biopsy samples were obtained from 108 captured P. keucopus mice, and tissues were obtained from 7 experimentally inoculated mice. A simple sample-processing procedure with proteinase K and detergent treatment was used in the PCR analysis. Overall, B. burgdorferi was detected in 29 of 108 (27%) P. leucopus mice by culture and in 31 of 108 (29%o) mice by PCR. As assessed by the kappa statistic, agreement between PCR and culture was high for ear and bladder (kappa = 0.80 and 0.65, respectively) and low for kidney and spleen (kappa = 0.37 and 0.03, respectively). While concordant results were obtained from 98
Scientific Journal of Kurdistan University of Medical Sciences, Jun 15, 2006
Background: Staphylococcus aureus is one of the most important etiological agent of hospital and ... more Background: Staphylococcus aureus is one of the most important etiological agent of hospital and community acquired infections. The enterotoxins and toxin shock syndrom toxin (TSST-1) are among the most common pathogenic determinants elaborated by this bacterium. They are also well-known for their super-antigenic properties and are commonly reffered to as pyrogenic toxins super antigens (PTSAgs) which exert deep influences in their host. Infections caused by drug-resistant S. aureus strains, mostly of hospital origin, are rapidly on the rise in many parts of the world. The incidence of TSST-1 elaborating strains are also very alarming. The aim of this investigation was to survey the prevalence of TSST-1 gene in the clinical isolates of S. aureus recovered from hospitalized pateints in Shohada hospital of Tabriz, Iran. Methods: During one year period, a total of 11353 clinical specimens, obtained from hospitalized patients were subjected to bacterial culture. Strains of Staphylococcu...
The Journal of Genes, Microbes and Immunity, 2014
Multidrug resistance (MDR) Acinetobacter baumannii, producing the extended-spectrum β-lactamases ... more Multidrug resistance (MDR) Acinetobacter baumannii, producing the extended-spectrum β-lactamases (ESBL) is one of the major concerns in hospitals. According to the infection reports caused by A.baumannii in Iran, the aim of this study is to identify the genes encoding the β-lactamases TEM and SHV in A.baumannii isolates in Tehran hospitals using PCR method. A total number of 200 clinical isolates A. baumanniii were diagnosed at first using culture methods and biochemical tests followed by antibiogram using 16 different antibiotics by disk diffusion method based on CLSI guidelines. The ESBL was determined by double disk synergy test (DDST) and the results were verified using combined disk method. PCR for determining the prevalence of genes was performed using specific primers for all isolates. There was a high antibiotic resistance among the isolates with a high MDR rate of 82.5% and 51% strains were producing ESBL. The minimum inhibitory concentration (MIC) rate of cefepime and ceftazidime in the isolates were 8-128 μg/ml. The prevalence of TEM and SHV were respectively 56% and 63%. These results indicate the necessity of a monitoring program to timely identify these strains to prevent the resistance spread and increase efficient antibiotics usage.
Journal of Periodontal Research, 1996
Culture and the polymerase chain reaction (PCR) were compared for detection of Borrelia burgdorfe... more Culture and the polymerase chain reaction (PCR) were compared for detection of Borrelia burgdorferi infection in wild-caught Peromyscus leucopus and experimentally inoculated C.B-17 scid/scid (severe combined immunodeficient) mice. PCR targeted highly conserved regions of the ospA gene and could detect one to five cultured organisms and 10 to 50 copies of molecularly cloned ospA DNA. Organs (kidney, spleen, and urinary bladder) and/or ear biopsy samples were obtained from 108 captured P. keucopus mice, and tissues were obtained from 7 experimentally inoculated mice. A simple sample-processing procedure with proteinase K and detergent treatment was used in the PCR analysis. Overall, B. burgdorferi was detected in 29 of 108 (27%) P. leucopus mice by culture and in 31 of 108 (29%o) mice by PCR. As assessed by the kappa statistic, agreement between PCR and culture was high for ear and bladder (kappa = 0.80 and 0.65, respectively) and low for kidney and spleen (kappa = 0.37 and 0.03, respectively). While concordant results were obtained from 98