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Papers by Noorhamdani Noorhamdani

Nucleation and Atmospheric Aerosols, 2023

Indonesian nursing journal of education and clinic (Jurnal pendidikan dan praktik keperawatan Indonesia), Jun 8, 2022

Introduction: Families who care for schizophrenia patients will experience various challenges, wh... more Introduction: Families who care for schizophrenia patients will experience various challenges, which are influenced by the family's knowledge and understanding of schizophrenia. This study aims to explore the family's understanding of schizophrenia in their families in rural families in Indonesia in 2020. Methods: This study uses a qualitative research design with a phenomenological approach, which was conducted in East Java Provincial Health Office from July to October 2020. The study participants were five family members who cared for a schizophrenia patient at home. Researchers conducted semistructured in-depth interviews with participants and analyzed using interpretative phenomenological analysis. Results: Based on the thematic analysis, three main themes were found, including 1) Family doesn't understand schizophrenia; 2) Family ignorance about how to care for the patient; and 3) Schizophrenia patients are considered a burden. Conclusions: The family's ignorance about schizophrenia risks causes their inaccuracy in treating patients. Nurses need to provide education about basic knowledge about schizophrenia and its treatment so that they can provide optimal care and be able to manage the burden they experience.

INDONESIAN NURSING JOURNAL OF EDUCATION AND CLINIC (INJEC)

Introduction: Families who care for schizophrenia patients will experience various challenges, wh... more Introduction: Families who care for schizophrenia patients will experience various challenges, which are influenced by the family's knowledge and understanding of schizophrenia. This study aims to explore the family's understanding of schizophrenia in their families in rural families in Indonesia in 2020.Methods: This study uses a qualitative research design with a phenomenological approach, which was conducted in East Java Provincial Health Office from July to October 2020. The study participants were five family members who cared for a schizophrenia patient at home. Researchers conducted semi-structured in-depth interviews with participants and analyzed using interpretative phenomenological analysis.Results: Based on the thematic analysis, three main themes were found, including 1) Family doesn't understand schizophrenia; 2) Family ignorance about how to care for the patient; and 3) Schizophrenia patients are considered a burden.Conclusions: The family's ignorance ...

THE PROCEEDINGS OF THE 4TH EPI INTERNATIONAL CONFERENCE ON SCIENCE AND ENGINEERING (EICSE) 2020

Endophytic bacteria can be a potential source of new bioactive molecules which has medicinal prop... more Endophytic bacteria can be a potential source of new bioactive molecules which has medicinal properties like their host plant. One of the Indonesian herbs is Sanrego plant (Lunasia amara Blanco), which is empirically used as antidiabetic by people in South Sulawesi. This study aimed to isolate the endophytic bacteria producing α-glucosidase inhibitor from the Sanrego plant. The endophytic bacteria isolated from leaf and stem of Sanrego plant, and screening of the target bacteria using thin layer chromatography (TLC) with scopoletin as the control due to scopoletin is the secondary metabolite of the Sanrego plant which has antidiabetic activity by in-silico. Alpha-glucosidase inhibitory test carried out by colorimetric method using pNPG substrate, at 405 nm wavelength compared to acarbose. This study found 14 isolates from Sanrego leaf and 25 isolates from Sanrego stem. The ten isolates of them showed α-glucosidase inhibitory effect, which were three isolates from leaves (D1-D3) and seven isolates from stems (B1-B7). The appearance of the endophytic bacteria was circular, convex, and yellow in color; while the cell morphology mostly were Grampositive bacteria. Statistical analysis indicated the D1 isolate had the highest inhibition value than the other isolates (ANOVA, p = 0.002). Interestingly, the inhibition value of the D1 isolate (8.67% ± 2.56) was higher compared to acarbose (3.73% ± 1.23). It can be concluded that the endophytic bacteria from the Sanrego plant produce α-glucosidase inhibitor compound, and the D1 isolate can be further investigated its potency as a source of α-glucosidase inhibitor.

Biomedical Physics & Engineering Express

Previous study using thoracic phantom for estimating fluid volume has been obtained which represe... more Previous study using thoracic phantom for estimating fluid volume has been obtained which represents the case of pleural effusion based on the size of the x-ray radiograph. The models are obtained in the form of three equations, the pleural effusion volume as a function of height, length times the height, and area of the radiograph image. The three models of estimation have high linearity with ratio value more than 0.988, higher than the modelling measurement using ultrasonography modality. The modelling is expected to give a contribution on developing method for helping clinicians estimate the pleural effusion volume as a basic for performing fluid aspiration and to monitor the therapy. However, because modelling is developed using phantoms, then to be applied clinically, further research is needed for its application to patients. The height function model yields correlation value of 0.966 and paired T-test value of 0.892. The height times length function model yields correlation v...

Nucleation and Atmospheric Aerosols, 2022

Endophytic bacteria can be a potential source of new bioactive molecules which has medicinal prop... more Endophytic bacteria can be a potential source of new bioactive molecules which has medicinal properties like their host plant. One of the Indonesian herbs is Sanrego plant (Lunasia amara Blanco), which is empirically used as antidiabetic by people in South Sulawesi. This study aimed to isolate the endophytic bacteria producing α-glucosidase inhibitor from the Sanrego plant. The endophytic bacteria isolated from leaf and stem of Sanrego plant, and screening of the target bacteria using thin layer chromatography (TLC) with scopoletin as the control due to scopoletin is the secondary metabolite of the Sanrego plant which has antidiabetic activity by in-silico. Alpha-glucosidase inhibitory test carried out by colorimetric method using pNPG substrate, at 405 nm wavelength compared to acarbose. This study found 14 isolates from Sanrego leaf and 25 isolates from Sanrego stem. The ten isolates of them showed α-glucosidase inhibitory effect, which were three isolates from leaves (D1-D3) and seven isolates from stems (B1-B7). The appearance of the endophytic bacteria was circular, convex, and yellow in color; while the cell morphology mostly were Grampositive bacteria. Statistical analysis indicated the D1 isolate had the highest inhibition value than the other isolates (ANOVA, p = 0.002). Interestingly, the inhibition value of the D1 isolate (8.67% ± 2.56) was higher compared to acarbose (3.73% ± 1.23). It can be concluded that the endophytic bacteria from the Sanrego plant produce α-glucosidase inhibitor compound, and the D1 isolate can be further investigated its potency as a source of α-glucosidase inhibitor.

Majalah Kesehatan, May 24, 2022

Staphylococcus epidermidis merupakan flora normal manusia yang banyak ditemukan di bagian kulit, ... more Staphylococcus epidermidis merupakan flora normal manusia yang banyak ditemukan di bagian kulit, membran mukosa, saluran pernapasan, serta pencernaan. Bakteri ini dapat menyebabkan infeksi oportunistik yang menyerang individu dengan imunitas rendah atau rentan, seperti penderita Acquired Immune Deficiency Syndrome (AIDS), pengguna narkotika, neonatus dan pasien rawat inap dalam waktu lama di rumah sakit. Staphylococcus epidermidis memiliki kemampuan untuk menempati permukaan tubuh dan alat-alat medis, yang dapat menyebabkan infeksi nosokomial, dengan membentuk biofilm yang sangat resisten. Daun jeruk purut (Citrus hystrix D.C.) merupakan komoditas yang banyak ditemukan di Indonesia, yang memiliki aktivitas antibakteri, serta memiliki nilai yang ekonomis dan mudah didapatkan. Tujuan dari penelitian ini ialah untuk membuktikan efektivitas dari minyak atsiri daun jeruk purut dalam menghambat dan membunuh pertumbuhan Staphylococcus epidermidis secara in vitro. Penelitian ini menggunakan metode dilusi tabung yang terdiri dari 12 perlakuan konsentrasi minyak atsiri yaitu 0

Tropical Medicine and Infectious Disease

Carbapenem-resistant Acinetobacter baumannii (A. baumannii)-calcoaceticus complex (CRAb-cc) is an... more Carbapenem-resistant Acinetobacter baumannii (A. baumannii)-calcoaceticus complex (CRAb-cc) is an important pathogen causing nosocomial infections worldwide; however, molecular epidemiology of the A. baumannii-calcoaceticus complex in Indonesian hospitals is scarce. This study aimed to determine the clonal relatedness of CRAb-cc in two tertiary care hospitals in Malang and Manado in Indonesia. The CRAb-cc isolates from routine clinical cultures in two tertiary care hospitals in Malang and Manado were identified using the Vitek2® system (bioMérieux, Lyon, France). Multi-locus variable-number tandem-repeat analysis (MLVA) typing, multi-locus sequence typing (MLST), clonal complex (CC), and phylogenetic tree analysis were conducted for a subset of isolates. Seventy-three CRAb-cc isolates were collected. The CRAb-cc isolates were frequently found among lower-respiratory-tract specimens. We detected the MLVA type (MT) 1, MT3, and MT4 CRAB-cc isolates belonging to the sequence type (ST) 6...

Research Journal of Pharmacy and Technology

Background: Carbapenem-resistant Acinetobacter baumannii (CRAB) is an important pathogenic bacter... more Background: Carbapenem-resistant Acinetobacter baumannii (CRAB) is an important pathogenic bacterium that can cause nosocomial infection in hospitalized patients with various manifestations. The purposes of this recent study were to determine the prevalence, antimicrobial susceptibility profiles, carbapenemase-producing phenotypic and genotypic of CRAB in two tertiary care hospitals in Indonesia. Methods: A. baumannii isolates collected from patient’s clinical cultures in two tertiary care hospitals in Malang and Manado were included. Identifications of meropenem-resistant A. baumannii isolates with the vitek2® system results, followed by a sensitivity test using 10 µg imipenem antibiotic disc according to CLSI guidelines to fulfill the criteria as CRAB isolates. We assessed carbapenemase-production using mCIM and eCIM, and determined the presence of blaKPC, blaNDM, blaOXA-23 carbapenemase resistance genes using simplex PCR. Results: 73 CRAB were collected from hospitalized patients...

Research Journal of Pharmacy and Technology

Indonesian have long empirical use of the Sanrego plant (Lunasia amara Blanco) as antidiabetic, b... more Indonesian have long empirical use of the Sanrego plant (Lunasia amara Blanco) as antidiabetic, but the active compounds of Sanrego that acts as antidiabetic is not yet known. This study aimed to know the active compound from the ethyl acetate extract (EEA) of Sanrego stems and leaves and predict its ability as an anti- diabetic by in-silico. The dried leaves and stems of Sanrego were grounded into powder and extracted using ethyl acetate. The active compounds were detected using thin-layer chromatography (TLC) and Liquid chromatography high-resolution mass spectrometry (LC-HRMS). Anti-diabetic activity was predicted by molecular docking approach compared to acarbose and vildagliptin. The TLC results showed that Sanrego EEA contained alkaloid and flavonoid compounds include scopoletin. The LC-HRMS results showed 11 active compounds in EEA and all of them had anti-diabetic activity. The detected main compounds were hesperidin, scopoletin, tangeritin, and trigonelline. Based on the re...

INTERNATIONAL CONFERENCE ON LIFE SCIENCES AND TECHNOLOGY (ICoLiST 2020), 2021

Antimicrobial Resistance & Infection Control, 2017

Background: Hand hygiene is recognized as an important measure to prevent healthcare-associated i... more Background: Hand hygiene is recognized as an important measure to prevent healthcare-associated infections. Hand hygiene adherence among healthcare workers is associated with their knowledge and perception. This study aimed to evaluate the effect of three different educational programs on improving hand hygiene compliance, knowledge, and perception among healthcare workers in a tertiary care hospital in Indonesia. Methods: The study was performed from May to October 2014 and divided into a pre-intervention, intervention, and post-intervention phase. This cluster randomized controlled trial allocated the implementation of three interventions to the departments, including role model training-pediatrics, active presentation-surgery, a combination of role model training and active presentation-internal medicine, and a control group-obstetrics-gynecology. Both direct observation and knowledge-perception survey of hand hygiene were performed using WHO tools. Results: Hand hygiene compliance was observed during 2,766 hand hygiene opportunities, and knowledge-perception was assessed among 196 participants in the pre-intervention and 88 in the post-intervention period. After intervention, the hand hygiene compliance rate improved significantly in pediatrics (24.1% to 43.7%; P < 0.001), internal medicine (5.2% to 18.5%; P < 0.001), and obstetrics-gynecology (10.1% to 20.5%; P < 0.001). The nurses' incorrect use of hand rub while wearing gloves increased as well (P < 0.001). The average knowledge score improved from 5.6 (SD = 2.1) to 6.2 (SD = 1.9) (P < 0.05). In the perception survey, "strong smell of hand alcohol" as a reason for non-compliance increased significantly in the departments with intervention (10.1% to 22.9%; P = 0.021). Conclusion: The educational programs improved the hand hygiene compliance and knowledge among healthcare workers in two out of three intervention departments in a limited-resource hospital in Indonesia. Role model training had the most impact in this setting. However, adjustments to the strategy are necessary to further improve hand hygiene.

Jurnal Gizi dan Dietetik Indonesia (Indonesian Journal of Nutrition and Dietetics), 2016

Background: Carrot is included in high beta carotene vegetable. Therefore, there were limited stu... more Background: Carrot is included in high beta carotene vegetable. Therefore, there were limited studies about the effect of cooking on nutrient content. Beta carotene found in carrots are provitamin A carotenoids that have strong antioxidant activity. Objectives: To determine the amount of beta carotene in carrots treated with two different cooking methods, that were, boiling and sautéing. Methods: A posttest-only control group design was used for this study. Samples of this study were local carrots varieties, and were selected by quota sampling. The carrots were divided into three groups, with fresh carrots (n=6) as control groups, and boiled carrots (n=6) and sautéed carrots (n=6) as treatment groups. Beta carotenes were extracted using petroleum ether-acetone solvents. They were separated by using column chromatography and measured by spectrophotometer at wave length 450nm. Data collected were analyzed with one way anova followed by post hoc tests duncan, independent t-test, and paired t-test. Results: The level of beta carotene between fresh carrots, boiled carrots, and sauteed carrots have signifi cant difference with p=0.013 (anova, p<0.05), whereas the level of beta carotene in boiled carrots and sauteed carrots have no signifi cantly difference with p=0.376 (independent t-test, p>0.05). However, there is a signifi cant decrease in the level of beta carotene in beginning and end groups from the boiling method with p=0.027, and the level of beta carotene in beginning and end groups from the sauteing method have a signifi cant decrease with p=0.020 (paired t-test, p<0.05). Conclusions: Boiling and sauteing decreased beta carotene levels in carrots.

Asian Pacific Journal of Reproduction, 2014

ABSTRACT To investigate whether Vigna unguiculata (V. Unguiculata, VU) able to reduced oxidative ... more ABSTRACT To investigate whether Vigna unguiculata (V. Unguiculata, VU) able to reduced oxidative stress in cardiac and modulate the aorta estrogen receptor-β of ovariectomized rats.

IOSR Journal of Pharmacy and Biological Sciences, 2014

Ventilator-associated pneumonia (VAP) is difficult to diagnose, expensive to treat, and is associ... more Ventilator-associated pneumonia (VAP) is difficult to diagnose, expensive to treat, and is associated with significant morbidity and mortality. The major part of VAP pathogenesis is the formation of a biofilm structure which also plays role in its resistance to treatment. A strategy to preventVAP isby removing the biofilm structure. The aim of this study is looking up the biofilm predictive protein of Enterobacter gergoviae (E. gergoviae) which is recognized by Secretory Immunoglobulin A (sIgA) as a mucosal immune response in VAP patient. This study used a tube adherence method for the detection of biofilms, protein profiling techniques with SDS PAGE and blotting for the evaluation of the antigen antibody response. The sIgAwere isolated from bronchial aspirate of VAP patient at ICU RSSA Malang. From two E. gergoviae samples, one sample biofilm positive (sample 1), and theother was biofilm negative (sample 2). The biofilm predictive protein was detected using SDS PAGE, and is discovered to be 15.

Asian Pacific Journal of Reproduction, 2014

Objective To investigate whether genistein is anti-inflammation and anti-angiogenesis in endometr... more Objective To investigate whether genistein is anti-inflammation and anti-angiogenesis in endometriosis cells.

Jurnal Kedokteran Brawijaya, 2004

Acinetobacter baumannii (Acb) is an opportunistic or a nosocomial pathogenic bacterium which atta... more Acinetobacter baumannii (Acb) is an opportunistic or a nosocomial pathogenic bacterium which attacks immunocompromised host. Colonization and infection often occur while hospitalized and could lead to pneumonia infection, urinary tract infection, meningitis, septicemia, and burn or surgical wound infections. In spite of the steady increase of Acb infection cases, little has been revealed about the Acb infection mechanism. The infection process is initiated by the adhesion of bacteria onto the host's cell, followed by multiplication, colonization and infection. Adhesion onto the host's cell is mediated by the adhesin molecule functioning as the virulence factor. Adhesin is usually found in the form of hemaglutinin protein which is bound to the receptor available at the host's cell surface. Target of research is to know weather the bacterium have hemaglutinin protein, by seeing the ability of agglutination red blood of mice 0.5%. Isolate of Acb coming from clinical specimen consisted of urine 35 isolates, sputum 27 isolates, pus 15 isolates, and blood 1 isolat. Acb which from environment 2 isolates. Result of positive hemaglutination test got from urine 13 / 35 (37.14%), sputum 8 / 27 (29.63%), pus 5 / 15 (33.33%) and Acb isolat coming from environment and blood all negativies. This result show Acb isolat of clinical specimen gave positive test has range from 29.63-37.14% that believed to have protein of hemaglutinin the functioningness as protein of adhesin to attach bacterium at hospes cells. Needed by research continue for the characteristic of the hemaglutinin protein and possibility of hemaglutination test of pathogenic bacterium

4th International Conference on Life Sciences and Technology, 2022

Biofilm is a mechanism of bacterial defense against antimicrobials that can cause resistance. Sta... more Biofilm is a mechanism of bacterial defense against antimicrobials that can cause resistance. Staphylococcus aureus is a biofilm-producing bacteria and the most often cause of skin and soft tissue infections. Therefore, efforts are needed to prevent the formation of Staphylococcus aureus biofilms. Basil leaves are herbal plants that contain eugenol and tannin compounds, which are thought to inhibit the formation of biofilms. This research is a laboratory experimental study that aims to prove the effect of basil leaves ethanol extract (Ocimum sanctum) on the establishment of Staphylococcus aureus biofilms with in vitro method and determine the minimum inhibitory biofilm concentration needed. In this study, the tube method with 7 different concentrations was used. The results of biofilm ring formation obtained and measured quantitatively using Mean Gray Value in Adobe Photoshop CS6. From the study’s results, is found that the increase in extract concentration is directly proportional ...

Majalah Kesehatan, 2021

Hospital-acquired infections (HAIs) merupakan infeksi yang terjadi pada pasien saat menerima pera... more Hospital-acquired infections (HAIs) merupakan infeksi yang terjadi pada pasien saat menerima perawatan di rumah sakit dalam waktu lebih dari 48 jam. Sebagian besar HAIs disebabkan oleh bakteri yang resisten terhadap antibiotik. Salah satu penyebabnya adalah bakteri Bacillus cereus . B . cereus memiliki dua jenis toksin yang menyebabkan gejala gastrointestinal. Pada individu immunocompromised , B . cereus dapat menyebabkan sejumlah infeksi sistemik dan lokal fatal . Upaya untuk mengurangi resistensi bakteri adalah dengan mengurangi penggunaan antibiotik yang tidak rasional, salah satunya dengan menggunakan alternatif pengobatan herbal. Daun jeruk purut ( Citrus hystrix D.C ) mengandung flavonoid, alkaloid, tannin, kuinon, dan terpenoid yang terbukti memiliki efek antimikroba. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri ekstrak etanol daun jeruk purut terhadap B . cereus secara in vitro . Dalam penelitian ini digunakan 7 perlakuan yaitu konsentrasi 3,125%; 6,25%; 1...

Nucleation and Atmospheric Aerosols, 2023

Indonesian nursing journal of education and clinic (Jurnal pendidikan dan praktik keperawatan Indonesia), Jun 8, 2022

Introduction: Families who care for schizophrenia patients will experience various challenges, wh... more Introduction: Families who care for schizophrenia patients will experience various challenges, which are influenced by the family's knowledge and understanding of schizophrenia. This study aims to explore the family's understanding of schizophrenia in their families in rural families in Indonesia in 2020. Methods: This study uses a qualitative research design with a phenomenological approach, which was conducted in East Java Provincial Health Office from July to October 2020. The study participants were five family members who cared for a schizophrenia patient at home. Researchers conducted semistructured in-depth interviews with participants and analyzed using interpretative phenomenological analysis. Results: Based on the thematic analysis, three main themes were found, including 1) Family doesn't understand schizophrenia; 2) Family ignorance about how to care for the patient; and 3) Schizophrenia patients are considered a burden. Conclusions: The family's ignorance about schizophrenia risks causes their inaccuracy in treating patients. Nurses need to provide education about basic knowledge about schizophrenia and its treatment so that they can provide optimal care and be able to manage the burden they experience.

INDONESIAN NURSING JOURNAL OF EDUCATION AND CLINIC (INJEC)

Introduction: Families who care for schizophrenia patients will experience various challenges, wh... more Introduction: Families who care for schizophrenia patients will experience various challenges, which are influenced by the family's knowledge and understanding of schizophrenia. This study aims to explore the family's understanding of schizophrenia in their families in rural families in Indonesia in 2020.Methods: This study uses a qualitative research design with a phenomenological approach, which was conducted in East Java Provincial Health Office from July to October 2020. The study participants were five family members who cared for a schizophrenia patient at home. Researchers conducted semi-structured in-depth interviews with participants and analyzed using interpretative phenomenological analysis.Results: Based on the thematic analysis, three main themes were found, including 1) Family doesn't understand schizophrenia; 2) Family ignorance about how to care for the patient; and 3) Schizophrenia patients are considered a burden.Conclusions: The family's ignorance ...

THE PROCEEDINGS OF THE 4TH EPI INTERNATIONAL CONFERENCE ON SCIENCE AND ENGINEERING (EICSE) 2020

Endophytic bacteria can be a potential source of new bioactive molecules which has medicinal prop... more Endophytic bacteria can be a potential source of new bioactive molecules which has medicinal properties like their host plant. One of the Indonesian herbs is Sanrego plant (Lunasia amara Blanco), which is empirically used as antidiabetic by people in South Sulawesi. This study aimed to isolate the endophytic bacteria producing α-glucosidase inhibitor from the Sanrego plant. The endophytic bacteria isolated from leaf and stem of Sanrego plant, and screening of the target bacteria using thin layer chromatography (TLC) with scopoletin as the control due to scopoletin is the secondary metabolite of the Sanrego plant which has antidiabetic activity by in-silico. Alpha-glucosidase inhibitory test carried out by colorimetric method using pNPG substrate, at 405 nm wavelength compared to acarbose. This study found 14 isolates from Sanrego leaf and 25 isolates from Sanrego stem. The ten isolates of them showed α-glucosidase inhibitory effect, which were three isolates from leaves (D1-D3) and seven isolates from stems (B1-B7). The appearance of the endophytic bacteria was circular, convex, and yellow in color; while the cell morphology mostly were Grampositive bacteria. Statistical analysis indicated the D1 isolate had the highest inhibition value than the other isolates (ANOVA, p = 0.002). Interestingly, the inhibition value of the D1 isolate (8.67% ± 2.56) was higher compared to acarbose (3.73% ± 1.23). It can be concluded that the endophytic bacteria from the Sanrego plant produce α-glucosidase inhibitor compound, and the D1 isolate can be further investigated its potency as a source of α-glucosidase inhibitor.

Biomedical Physics & Engineering Express

Previous study using thoracic phantom for estimating fluid volume has been obtained which represe... more Previous study using thoracic phantom for estimating fluid volume has been obtained which represents the case of pleural effusion based on the size of the x-ray radiograph. The models are obtained in the form of three equations, the pleural effusion volume as a function of height, length times the height, and area of the radiograph image. The three models of estimation have high linearity with ratio value more than 0.988, higher than the modelling measurement using ultrasonography modality. The modelling is expected to give a contribution on developing method for helping clinicians estimate the pleural effusion volume as a basic for performing fluid aspiration and to monitor the therapy. However, because modelling is developed using phantoms, then to be applied clinically, further research is needed for its application to patients. The height function model yields correlation value of 0.966 and paired T-test value of 0.892. The height times length function model yields correlation v...

Nucleation and Atmospheric Aerosols, 2022

Endophytic bacteria can be a potential source of new bioactive molecules which has medicinal prop... more Endophytic bacteria can be a potential source of new bioactive molecules which has medicinal properties like their host plant. One of the Indonesian herbs is Sanrego plant (Lunasia amara Blanco), which is empirically used as antidiabetic by people in South Sulawesi. This study aimed to isolate the endophytic bacteria producing α-glucosidase inhibitor from the Sanrego plant. The endophytic bacteria isolated from leaf and stem of Sanrego plant, and screening of the target bacteria using thin layer chromatography (TLC) with scopoletin as the control due to scopoletin is the secondary metabolite of the Sanrego plant which has antidiabetic activity by in-silico. Alpha-glucosidase inhibitory test carried out by colorimetric method using pNPG substrate, at 405 nm wavelength compared to acarbose. This study found 14 isolates from Sanrego leaf and 25 isolates from Sanrego stem. The ten isolates of them showed α-glucosidase inhibitory effect, which were three isolates from leaves (D1-D3) and seven isolates from stems (B1-B7). The appearance of the endophytic bacteria was circular, convex, and yellow in color; while the cell morphology mostly were Grampositive bacteria. Statistical analysis indicated the D1 isolate had the highest inhibition value than the other isolates (ANOVA, p = 0.002). Interestingly, the inhibition value of the D1 isolate (8.67% ± 2.56) was higher compared to acarbose (3.73% ± 1.23). It can be concluded that the endophytic bacteria from the Sanrego plant produce α-glucosidase inhibitor compound, and the D1 isolate can be further investigated its potency as a source of α-glucosidase inhibitor.

Majalah Kesehatan, May 24, 2022

Staphylococcus epidermidis merupakan flora normal manusia yang banyak ditemukan di bagian kulit, ... more Staphylococcus epidermidis merupakan flora normal manusia yang banyak ditemukan di bagian kulit, membran mukosa, saluran pernapasan, serta pencernaan. Bakteri ini dapat menyebabkan infeksi oportunistik yang menyerang individu dengan imunitas rendah atau rentan, seperti penderita Acquired Immune Deficiency Syndrome (AIDS), pengguna narkotika, neonatus dan pasien rawat inap dalam waktu lama di rumah sakit. Staphylococcus epidermidis memiliki kemampuan untuk menempati permukaan tubuh dan alat-alat medis, yang dapat menyebabkan infeksi nosokomial, dengan membentuk biofilm yang sangat resisten. Daun jeruk purut (Citrus hystrix D.C.) merupakan komoditas yang banyak ditemukan di Indonesia, yang memiliki aktivitas antibakteri, serta memiliki nilai yang ekonomis dan mudah didapatkan. Tujuan dari penelitian ini ialah untuk membuktikan efektivitas dari minyak atsiri daun jeruk purut dalam menghambat dan membunuh pertumbuhan Staphylococcus epidermidis secara in vitro. Penelitian ini menggunakan metode dilusi tabung yang terdiri dari 12 perlakuan konsentrasi minyak atsiri yaitu 0

Tropical Medicine and Infectious Disease

Carbapenem-resistant Acinetobacter baumannii (A. baumannii)-calcoaceticus complex (CRAb-cc) is an... more Carbapenem-resistant Acinetobacter baumannii (A. baumannii)-calcoaceticus complex (CRAb-cc) is an important pathogen causing nosocomial infections worldwide; however, molecular epidemiology of the A. baumannii-calcoaceticus complex in Indonesian hospitals is scarce. This study aimed to determine the clonal relatedness of CRAb-cc in two tertiary care hospitals in Malang and Manado in Indonesia. The CRAb-cc isolates from routine clinical cultures in two tertiary care hospitals in Malang and Manado were identified using the Vitek2® system (bioMérieux, Lyon, France). Multi-locus variable-number tandem-repeat analysis (MLVA) typing, multi-locus sequence typing (MLST), clonal complex (CC), and phylogenetic tree analysis were conducted for a subset of isolates. Seventy-three CRAb-cc isolates were collected. The CRAb-cc isolates were frequently found among lower-respiratory-tract specimens. We detected the MLVA type (MT) 1, MT3, and MT4 CRAB-cc isolates belonging to the sequence type (ST) 6...

Research Journal of Pharmacy and Technology

Background: Carbapenem-resistant Acinetobacter baumannii (CRAB) is an important pathogenic bacter... more Background: Carbapenem-resistant Acinetobacter baumannii (CRAB) is an important pathogenic bacterium that can cause nosocomial infection in hospitalized patients with various manifestations. The purposes of this recent study were to determine the prevalence, antimicrobial susceptibility profiles, carbapenemase-producing phenotypic and genotypic of CRAB in two tertiary care hospitals in Indonesia. Methods: A. baumannii isolates collected from patient’s clinical cultures in two tertiary care hospitals in Malang and Manado were included. Identifications of meropenem-resistant A. baumannii isolates with the vitek2® system results, followed by a sensitivity test using 10 µg imipenem antibiotic disc according to CLSI guidelines to fulfill the criteria as CRAB isolates. We assessed carbapenemase-production using mCIM and eCIM, and determined the presence of blaKPC, blaNDM, blaOXA-23 carbapenemase resistance genes using simplex PCR. Results: 73 CRAB were collected from hospitalized patients...

Research Journal of Pharmacy and Technology

Indonesian have long empirical use of the Sanrego plant (Lunasia amara Blanco) as antidiabetic, b... more Indonesian have long empirical use of the Sanrego plant (Lunasia amara Blanco) as antidiabetic, but the active compounds of Sanrego that acts as antidiabetic is not yet known. This study aimed to know the active compound from the ethyl acetate extract (EEA) of Sanrego stems and leaves and predict its ability as an anti- diabetic by in-silico. The dried leaves and stems of Sanrego were grounded into powder and extracted using ethyl acetate. The active compounds were detected using thin-layer chromatography (TLC) and Liquid chromatography high-resolution mass spectrometry (LC-HRMS). Anti-diabetic activity was predicted by molecular docking approach compared to acarbose and vildagliptin. The TLC results showed that Sanrego EEA contained alkaloid and flavonoid compounds include scopoletin. The LC-HRMS results showed 11 active compounds in EEA and all of them had anti-diabetic activity. The detected main compounds were hesperidin, scopoletin, tangeritin, and trigonelline. Based on the re...

INTERNATIONAL CONFERENCE ON LIFE SCIENCES AND TECHNOLOGY (ICoLiST 2020), 2021

Antimicrobial Resistance & Infection Control, 2017

Background: Hand hygiene is recognized as an important measure to prevent healthcare-associated i... more Background: Hand hygiene is recognized as an important measure to prevent healthcare-associated infections. Hand hygiene adherence among healthcare workers is associated with their knowledge and perception. This study aimed to evaluate the effect of three different educational programs on improving hand hygiene compliance, knowledge, and perception among healthcare workers in a tertiary care hospital in Indonesia. Methods: The study was performed from May to October 2014 and divided into a pre-intervention, intervention, and post-intervention phase. This cluster randomized controlled trial allocated the implementation of three interventions to the departments, including role model training-pediatrics, active presentation-surgery, a combination of role model training and active presentation-internal medicine, and a control group-obstetrics-gynecology. Both direct observation and knowledge-perception survey of hand hygiene were performed using WHO tools. Results: Hand hygiene compliance was observed during 2,766 hand hygiene opportunities, and knowledge-perception was assessed among 196 participants in the pre-intervention and 88 in the post-intervention period. After intervention, the hand hygiene compliance rate improved significantly in pediatrics (24.1% to 43.7%; P < 0.001), internal medicine (5.2% to 18.5%; P < 0.001), and obstetrics-gynecology (10.1% to 20.5%; P < 0.001). The nurses' incorrect use of hand rub while wearing gloves increased as well (P < 0.001). The average knowledge score improved from 5.6 (SD = 2.1) to 6.2 (SD = 1.9) (P < 0.05). In the perception survey, "strong smell of hand alcohol" as a reason for non-compliance increased significantly in the departments with intervention (10.1% to 22.9%; P = 0.021). Conclusion: The educational programs improved the hand hygiene compliance and knowledge among healthcare workers in two out of three intervention departments in a limited-resource hospital in Indonesia. Role model training had the most impact in this setting. However, adjustments to the strategy are necessary to further improve hand hygiene.

Jurnal Gizi dan Dietetik Indonesia (Indonesian Journal of Nutrition and Dietetics), 2016

Background: Carrot is included in high beta carotene vegetable. Therefore, there were limited stu... more Background: Carrot is included in high beta carotene vegetable. Therefore, there were limited studies about the effect of cooking on nutrient content. Beta carotene found in carrots are provitamin A carotenoids that have strong antioxidant activity. Objectives: To determine the amount of beta carotene in carrots treated with two different cooking methods, that were, boiling and sautéing. Methods: A posttest-only control group design was used for this study. Samples of this study were local carrots varieties, and were selected by quota sampling. The carrots were divided into three groups, with fresh carrots (n=6) as control groups, and boiled carrots (n=6) and sautéed carrots (n=6) as treatment groups. Beta carotenes were extracted using petroleum ether-acetone solvents. They were separated by using column chromatography and measured by spectrophotometer at wave length 450nm. Data collected were analyzed with one way anova followed by post hoc tests duncan, independent t-test, and paired t-test. Results: The level of beta carotene between fresh carrots, boiled carrots, and sauteed carrots have signifi cant difference with p=0.013 (anova, p<0.05), whereas the level of beta carotene in boiled carrots and sauteed carrots have no signifi cantly difference with p=0.376 (independent t-test, p>0.05). However, there is a signifi cant decrease in the level of beta carotene in beginning and end groups from the boiling method with p=0.027, and the level of beta carotene in beginning and end groups from the sauteing method have a signifi cant decrease with p=0.020 (paired t-test, p<0.05). Conclusions: Boiling and sauteing decreased beta carotene levels in carrots.

Asian Pacific Journal of Reproduction, 2014

ABSTRACT To investigate whether Vigna unguiculata (V. Unguiculata, VU) able to reduced oxidative ... more ABSTRACT To investigate whether Vigna unguiculata (V. Unguiculata, VU) able to reduced oxidative stress in cardiac and modulate the aorta estrogen receptor-β of ovariectomized rats.

IOSR Journal of Pharmacy and Biological Sciences, 2014

Ventilator-associated pneumonia (VAP) is difficult to diagnose, expensive to treat, and is associ... more Ventilator-associated pneumonia (VAP) is difficult to diagnose, expensive to treat, and is associated with significant morbidity and mortality. The major part of VAP pathogenesis is the formation of a biofilm structure which also plays role in its resistance to treatment. A strategy to preventVAP isby removing the biofilm structure. The aim of this study is looking up the biofilm predictive protein of Enterobacter gergoviae (E. gergoviae) which is recognized by Secretory Immunoglobulin A (sIgA) as a mucosal immune response in VAP patient. This study used a tube adherence method for the detection of biofilms, protein profiling techniques with SDS PAGE and blotting for the evaluation of the antigen antibody response. The sIgAwere isolated from bronchial aspirate of VAP patient at ICU RSSA Malang. From two E. gergoviae samples, one sample biofilm positive (sample 1), and theother was biofilm negative (sample 2). The biofilm predictive protein was detected using SDS PAGE, and is discovered to be 15.

Asian Pacific Journal of Reproduction, 2014

Objective To investigate whether genistein is anti-inflammation and anti-angiogenesis in endometr... more Objective To investigate whether genistein is anti-inflammation and anti-angiogenesis in endometriosis cells.

Jurnal Kedokteran Brawijaya, 2004

Acinetobacter baumannii (Acb) is an opportunistic or a nosocomial pathogenic bacterium which atta... more Acinetobacter baumannii (Acb) is an opportunistic or a nosocomial pathogenic bacterium which attacks immunocompromised host. Colonization and infection often occur while hospitalized and could lead to pneumonia infection, urinary tract infection, meningitis, septicemia, and burn or surgical wound infections. In spite of the steady increase of Acb infection cases, little has been revealed about the Acb infection mechanism. The infection process is initiated by the adhesion of bacteria onto the host's cell, followed by multiplication, colonization and infection. Adhesion onto the host's cell is mediated by the adhesin molecule functioning as the virulence factor. Adhesin is usually found in the form of hemaglutinin protein which is bound to the receptor available at the host's cell surface. Target of research is to know weather the bacterium have hemaglutinin protein, by seeing the ability of agglutination red blood of mice 0.5%. Isolate of Acb coming from clinical specimen consisted of urine 35 isolates, sputum 27 isolates, pus 15 isolates, and blood 1 isolat. Acb which from environment 2 isolates. Result of positive hemaglutination test got from urine 13 / 35 (37.14%), sputum 8 / 27 (29.63%), pus 5 / 15 (33.33%) and Acb isolat coming from environment and blood all negativies. This result show Acb isolat of clinical specimen gave positive test has range from 29.63-37.14% that believed to have protein of hemaglutinin the functioningness as protein of adhesin to attach bacterium at hospes cells. Needed by research continue for the characteristic of the hemaglutinin protein and possibility of hemaglutination test of pathogenic bacterium

4th International Conference on Life Sciences and Technology, 2022

Biofilm is a mechanism of bacterial defense against antimicrobials that can cause resistance. Sta... more Biofilm is a mechanism of bacterial defense against antimicrobials that can cause resistance. Staphylococcus aureus is a biofilm-producing bacteria and the most often cause of skin and soft tissue infections. Therefore, efforts are needed to prevent the formation of Staphylococcus aureus biofilms. Basil leaves are herbal plants that contain eugenol and tannin compounds, which are thought to inhibit the formation of biofilms. This research is a laboratory experimental study that aims to prove the effect of basil leaves ethanol extract (Ocimum sanctum) on the establishment of Staphylococcus aureus biofilms with in vitro method and determine the minimum inhibitory biofilm concentration needed. In this study, the tube method with 7 different concentrations was used. The results of biofilm ring formation obtained and measured quantitatively using Mean Gray Value in Adobe Photoshop CS6. From the study’s results, is found that the increase in extract concentration is directly proportional ...

Majalah Kesehatan, 2021

Hospital-acquired infections (HAIs) merupakan infeksi yang terjadi pada pasien saat menerima pera... more Hospital-acquired infections (HAIs) merupakan infeksi yang terjadi pada pasien saat menerima perawatan di rumah sakit dalam waktu lebih dari 48 jam. Sebagian besar HAIs disebabkan oleh bakteri yang resisten terhadap antibiotik. Salah satu penyebabnya adalah bakteri Bacillus cereus . B . cereus memiliki dua jenis toksin yang menyebabkan gejala gastrointestinal. Pada individu immunocompromised , B . cereus dapat menyebabkan sejumlah infeksi sistemik dan lokal fatal . Upaya untuk mengurangi resistensi bakteri adalah dengan mengurangi penggunaan antibiotik yang tidak rasional, salah satunya dengan menggunakan alternatif pengobatan herbal. Daun jeruk purut ( Citrus hystrix D.C ) mengandung flavonoid, alkaloid, tannin, kuinon, dan terpenoid yang terbukti memiliki efek antimikroba. Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri ekstrak etanol daun jeruk purut terhadap B . cereus secara in vitro . Dalam penelitian ini digunakan 7 perlakuan yaitu konsentrasi 3,125%; 6,25%; 1...