Ofijan Tesfaye - Academia.edu (original) (raw)

Papers by Ofijan Tesfaye

Heliyon, Apr 1, 2024

Honey is a natural product that is made by bees from the nectar of flowering plants. There is a f... more Honey is a natural product that is made by bees from the nectar of flowering plants. There is a flora preference by bees. Like other foods ready to eat,honey can be prone to microbial contamination. Honey plant sources can be analyzed from the composition of pollen grains in honey samples. The objective of this study was to assess microbial safety and floral origin of the honey samples. For this study, honey samples were purchased from local market, and collected from hives (fresh honey) in Western Oromia. Floral analysis was determined using harmonized method of melissopalynology. Microbiological safety was assessed through the pour plate procedures from the first serial dilution on a total of 45 honey sample sizes.The melissopalynological analysis demonstrated that A. melliferahoney purchased from the market(AMMH) was considered a multi-floral type while A. mellifera fresh honey (AMFH) cropped directly from the hive and M.beccarii honey purchased from the market (MBMH) was dominated pollen from Coffee arabica (68% of its pollen grain counted) and Guizotia scabra (50.53 % of its pollen grain counted) plant,respectively. The Aerobic mesophilic bacteria, Staphylococci, Yeast, Mould, and Aerobic sporeforming bacteria were found below the standard countable level (<30 cfu/plate) from A. mellifera and M.beccarii honey bought from the market, while A. mellifera honey collected directly fromthe hive became free of any microbial contamination. C.arabica and G.scabra are major honeyplants and their honey can be harvested in February and October, respectively. Furthermore,Vernonia amygdalina, Eucalyptus spp, Combretum molle, Trifolium ruppelianum, and Syzgium guineense were honey plants analyzed from multifloral market honey even though, their pollen
dominance varies. M. beccarii visits herbaceous flora whilst A. mellifera visits all floral types. Thelevel of contamination of the honey samples from the study area was very low showing its safety.

International Journal of Forestry Research, Jul 19, 2023

Beekeeping has signifcantly contributed to environmental conservation and the preservation of nat... more Beekeeping has signifcantly contributed to environmental conservation and the preservation of natural resources. Although the quality and quantity of fora available play a major role in the success of the sector, the botanical makeup of natural vegetation varies greatly. Tis study was conducted targeting the identifcation and documentation of major honeybee foras and their fowering calendar. Midland and lowland agroecologies were purposively selected. Continuous feld plant registration was performed. Melissopalynological analysis from bee pollen and honey were used to identify foral origin. Field observations identifed 59 and 63 plants in the midland and lowlands, respectively. Season 1 had the highest pollen yields, ranging from 11051.8 ± 56.4 g (midlands) to 878.3 ± 18.3 g (lowlands), while season 4 ranged from 16.8 ± 6.3 g (midlands) to 15.6 ± 7.4 g (lowlands) and had the lowest pollen yield. In both regions, February, March, July, and August are the months when pollen is not brought into the hive and could be used as starvation periods. A total of 1430.8 ± 75.4 and 1291.8 ± 71.4 g of bee pollen/hive were collected throughout the year in midland and lowland, respectively, and Asteraceae was the richest family accounting around 90% of pollen weight. In both agroecologies, honey is harvested three times a year. In the midland, monoforal honey, namely, Guizotia spp (64.42%) and Croton macrostychus (47.42%), was harvested in November and May, respectively, while honey harvested in February was multiforal type. Similarly, in the lowlands, monoforal honey of Guizotia spp (51.85%), Cofee arabica (55.22%), and Croton macrostychus (50.42%) was harvested in December, March, and June, respectively. Based on the results, Bidens prestinaria, Bidens pilosa, Guizotia spp, C. macrostachyus, Eucalyptus spp, Lepidium sativum, Zea mays, Hypostes trifolia, Vernonia spp, Trifolium spp, Helianthus annuus, C. arabica, Brassica abyssinica, Andropogon abyssinicus, Sorghum bicolor, Cordia africana, Syzygium guineense, and Terminalia spp are major bee plants. It is found that the study area is rich in bee plant diversity and hence has a potential for honey production.

International journal of food science, Jul 15, 2023

The antioxidant properties of natural honey primarily rely on the floral origin from which nectar... more The antioxidant properties of natural honey primarily rely on the floral origin from which nectar is collected by bees. Thus, the current activity evaluated the antioxidant properties of honey based on its floral type. The honey floral origin was verified by the melissopalynological technique. Antioxidant properties were determined by using standard procedures and analyzed by SAS. Six unifloral honey types with their harvesting month were identified. Accordingly, Guizotia (74% of pollen frequency), Coffea arabica (68%), Vernonia (90%), Croton macrostachyus (64%), Schefflera abyssinica (100%), and Eucalyptus (100%) were cropped in November, February, February, May, April, and June separately. Statistically, a variation (p < 0:05) in antioxidant parameters was displayed between unifloral honeys. Vernonia honey exhibited the maximum total phenol (77:2 ± 0:7), total flavonoid (65:0 ± 3:8), and total antioxidant content (65:4 ± 0:3). On the other hand, S. abyssinica honey recorded the least total phenol content (24:1 ± 0:4), total flavonoid content (18:6 ± 2:7), and total antioxidant content (5:6 ± 0:5). Statistical analysis showed a positive correlation between all the tested antioxidant parameters. Thus, the current study indicated that all the tested Ethiopian unifloral honey had good sources of antioxidants with the most Vernonia honey followed by C. macrostachyus whereas S. abyssinica honey had the least followed by Eucalyptus.

Research Square (Research Square), Jan 16, 2023

Background Honey is a natural sweetener and viscous food which is synthesized from the nectar of ... more Background Honey is a natural sweetener and viscous food which is synthesized from the nectar of owering plants by bees. As a worldwide, it has been traditionally used for incurring different diseases. However, In recent times, the emergence of multi and extended drug-resistant human pathogens called for an urgent search for more effective sources of natural products to treat infectious diseases. The biological activity of honey primarily relies on its botanical and entomological origin. Hence, the objective of this study was to screen the in vitro antibacterial activity of honey based on botanical origin produced in Ethiopia. Methods Botanical origin was identi ed by the Melisopalynological analysis method. The inhibition zone was determined by agar well diffusion assay. Minimum inhibitory concentration was done by broth diffusion. Result Six different mono oral honey namely: Guizotia (75.3% pollen grain counted), Coffee arabica (68.6%), Vernonia (90.5%), Sche era abyssinica (100%), Croton macrostachyus (64.4%) and Eucalyptus (100%) honey was harvested through December, February, February, April, May, and June respectively from the study area. All the mono oral honey inhibited the growth of all the bacterial strains even though the diameter of the inhibition zone varied. C. macrostachyus, Vernonia, Guizotia, and C. arabica were comparatively more whilst S. abyssinica was the least effective against all the tested bacterial strains. The grand mean inhibition zone ranged from 17.0 ± 1 mm by C. macrostachyus to 10.5 ± 1 mm by S. abyssinica against all the bacterial strains. Besides, the MIC of botanical honey type ranged from 11.7 ± 3.3% w/v by C. macrostachyus against S. aureus to 37.5 ± 1% w/v by S. abyssinica against P. aeruginosa. Conclusion The current result indicated that there is a disparity in antibacterial properties between mono oral honey. The more effective honey against the tested bacterial strain were those produced from the nectar of Ethiopian medicinal plants; based on literatures. Therefore, further invivo and in-vitro research would be expected from the concerned researchers on the association between the biological compounds present in each part of medicinal plants and honey samples.

Water, 2019

With increasing population, the need for research ideas on the field of reducing wastage of water... more With increasing population, the need for research ideas on the field of reducing wastage of water can save a big amount of water, money, time, and energy. Water leakage (WL) is an essential problem in the field of water supply field. This research is focused on real water loss in the water distribution system located in Ethiopia. Top-down and bursts and background estimates (BABE) methodology is performed to assess the data and the calibration process of the WL variables. The top-down method assists to quantify the water loss by the record and observation throughout the distribution network. In addition, the BABE approach gives a specific water leakage and burst information. The geometrical mean method is used to forecast the population up to 2023 along with their fiscal value by the uniform tariff method. With respect to the revenue lost, 42575 Br and 42664 Br or in 1562$ and 1566$ were lost in 2017 and 2018, respectively. The next five-year population was forecasted to estimate th...

International Journal of Food Properties, Jan 4, 2022

International Journal of Forestry Research

Beekeeping has significantly contributed to environmental conservation and the preservation of na... more Beekeeping has significantly contributed to environmental conservation and the preservation of natural resources. Although the quality and quantity of flora available play a major role in the success of the sector, the botanical makeup of natural vegetation varies greatly. This study was conducted targeting the identification and documentation of major honeybee floras and their flowering calendar. Midland and lowland agroecologies were purposively selected. Continuous field plant registration was performed. Melissopalynological analysis from bee pollen and honey were used to identify floral origin. Field observations identified 59 and 63 plants in the midland and lowlands, respectively. Season 1 had the highest pollen yields, ranging from 11051.8 ± 56.4 g (midlands) to 878.3 ± 18.3 g (lowlands), while season 4 ranged from 16.8 ± 6.3 g (midlands) to 15.6 ± 7.4 g (lowlands) and had the lowest pollen yield. In both regions, February, March, July, and August are the months when pollen i...

International Journal of Food Science

The antioxidant properties of natural honey primarily rely on the floral origin from which nectar... more The antioxidant properties of natural honey primarily rely on the floral origin from which nectar is collected by bees. Thus, the current activity evaluated the antioxidant properties of honey based on its floral type. The honey floral origin was verified by the melissopalynological technique. Antioxidant properties were determined by using standard procedures and analyzed by SAS. Six unifloral honey types with their harvesting month were identified. Accordingly, Guizotia (74% of pollen frequency), Coffea arabica (68%), Vernonia (90%), Croton macrostachyus (64%), Schefflera abyssinica (100%), and Eucalyptus (100%) were cropped in November, February, February, May, April, and June separately. Statistically, a variation ( p < 0.05 ) in antioxidant parameters was displayed between unifloral honeys. Vernonia honey exhibited the maximum total phenol ( 77.2 ± 0.7 ), total flavonoid ( 65.0 ± 3.8 ), and total antioxidant content ( 65.4 ± 0.3 ). On the other hand, S. abyssinica honey reco...

Background Honey is a natural sweetener and viscous food which is synthesized from the nectar of ... more Background Honey is a natural sweetener and viscous food which is synthesized from the nectar of flowering plants by bees. As a worldwide, it has been traditionally used for incurring different diseases. However, In recent times, the emergence of multi and extended drug-resistant human pathogens called for an urgent search for more effective sources of natural products to treat infectious diseases. The biological activity of honey primarily relies on its botanical and entomological origin. Hence, the objective of this study was to screen the in vitro antibacterial activity of honey based on botanical origin produced in Ethiopia. Methods Botanical origin was identified by the Melisopalynological analysis method. The inhibition zone was determined by agar well diffusion assay. Minimum inhibitory concentration was done by broth diffusion. Result Six different monofloral honey namely: Guizotia (75.3% pollen grain counted), Coffee arabica (68.6%), Vernonia (90.5%), Schefflera abyssinica ...

Journal of Biology, Agriculture and Healthcare, 2019

International Journal of Food Properties, 2022

Heliyon, Apr 1, 2024

Honey is a natural product that is made by bees from the nectar of flowering plants. There is a f... more Honey is a natural product that is made by bees from the nectar of flowering plants. There is a flora preference by bees. Like other foods ready to eat,honey can be prone to microbial contamination. Honey plant sources can be analyzed from the composition of pollen grains in honey samples. The objective of this study was to assess microbial safety and floral origin of the honey samples. For this study, honey samples were purchased from local market, and collected from hives (fresh honey) in Western Oromia. Floral analysis was determined using harmonized method of melissopalynology. Microbiological safety was assessed through the pour plate procedures from the first serial dilution on a total of 45 honey sample sizes.The melissopalynological analysis demonstrated that A. melliferahoney purchased from the market(AMMH) was considered a multi-floral type while A. mellifera fresh honey (AMFH) cropped directly from the hive and M.beccarii honey purchased from the market (MBMH) was dominated pollen from Coffee arabica (68% of its pollen grain counted) and Guizotia scabra (50.53 % of its pollen grain counted) plant,respectively. The Aerobic mesophilic bacteria, Staphylococci, Yeast, Mould, and Aerobic sporeforming bacteria were found below the standard countable level (<30 cfu/plate) from A. mellifera and M.beccarii honey bought from the market, while A. mellifera honey collected directly fromthe hive became free of any microbial contamination. C.arabica and G.scabra are major honeyplants and their honey can be harvested in February and October, respectively. Furthermore,Vernonia amygdalina, Eucalyptus spp, Combretum molle, Trifolium ruppelianum, and Syzgium guineense were honey plants analyzed from multifloral market honey even though, their pollen
dominance varies. M. beccarii visits herbaceous flora whilst A. mellifera visits all floral types. Thelevel of contamination of the honey samples from the study area was very low showing its safety.

International Journal of Forestry Research, Jul 19, 2023

Beekeeping has signifcantly contributed to environmental conservation and the preservation of nat... more Beekeeping has signifcantly contributed to environmental conservation and the preservation of natural resources. Although the quality and quantity of fora available play a major role in the success of the sector, the botanical makeup of natural vegetation varies greatly. Tis study was conducted targeting the identifcation and documentation of major honeybee foras and their fowering calendar. Midland and lowland agroecologies were purposively selected. Continuous feld plant registration was performed. Melissopalynological analysis from bee pollen and honey were used to identify foral origin. Field observations identifed 59 and 63 plants in the midland and lowlands, respectively. Season 1 had the highest pollen yields, ranging from 11051.8 ± 56.4 g (midlands) to 878.3 ± 18.3 g (lowlands), while season 4 ranged from 16.8 ± 6.3 g (midlands) to 15.6 ± 7.4 g (lowlands) and had the lowest pollen yield. In both regions, February, March, July, and August are the months when pollen is not brought into the hive and could be used as starvation periods. A total of 1430.8 ± 75.4 and 1291.8 ± 71.4 g of bee pollen/hive were collected throughout the year in midland and lowland, respectively, and Asteraceae was the richest family accounting around 90% of pollen weight. In both agroecologies, honey is harvested three times a year. In the midland, monoforal honey, namely, Guizotia spp (64.42%) and Croton macrostychus (47.42%), was harvested in November and May, respectively, while honey harvested in February was multiforal type. Similarly, in the lowlands, monoforal honey of Guizotia spp (51.85%), Cofee arabica (55.22%), and Croton macrostychus (50.42%) was harvested in December, March, and June, respectively. Based on the results, Bidens prestinaria, Bidens pilosa, Guizotia spp, C. macrostachyus, Eucalyptus spp, Lepidium sativum, Zea mays, Hypostes trifolia, Vernonia spp, Trifolium spp, Helianthus annuus, C. arabica, Brassica abyssinica, Andropogon abyssinicus, Sorghum bicolor, Cordia africana, Syzygium guineense, and Terminalia spp are major bee plants. It is found that the study area is rich in bee plant diversity and hence has a potential for honey production.

International journal of food science, Jul 15, 2023

The antioxidant properties of natural honey primarily rely on the floral origin from which nectar... more The antioxidant properties of natural honey primarily rely on the floral origin from which nectar is collected by bees. Thus, the current activity evaluated the antioxidant properties of honey based on its floral type. The honey floral origin was verified by the melissopalynological technique. Antioxidant properties were determined by using standard procedures and analyzed by SAS. Six unifloral honey types with their harvesting month were identified. Accordingly, Guizotia (74% of pollen frequency), Coffea arabica (68%), Vernonia (90%), Croton macrostachyus (64%), Schefflera abyssinica (100%), and Eucalyptus (100%) were cropped in November, February, February, May, April, and June separately. Statistically, a variation (p < 0:05) in antioxidant parameters was displayed between unifloral honeys. Vernonia honey exhibited the maximum total phenol (77:2 ± 0:7), total flavonoid (65:0 ± 3:8), and total antioxidant content (65:4 ± 0:3). On the other hand, S. abyssinica honey recorded the least total phenol content (24:1 ± 0:4), total flavonoid content (18:6 ± 2:7), and total antioxidant content (5:6 ± 0:5). Statistical analysis showed a positive correlation between all the tested antioxidant parameters. Thus, the current study indicated that all the tested Ethiopian unifloral honey had good sources of antioxidants with the most Vernonia honey followed by C. macrostachyus whereas S. abyssinica honey had the least followed by Eucalyptus.

Research Square (Research Square), Jan 16, 2023

Background Honey is a natural sweetener and viscous food which is synthesized from the nectar of ... more Background Honey is a natural sweetener and viscous food which is synthesized from the nectar of owering plants by bees. As a worldwide, it has been traditionally used for incurring different diseases. However, In recent times, the emergence of multi and extended drug-resistant human pathogens called for an urgent search for more effective sources of natural products to treat infectious diseases. The biological activity of honey primarily relies on its botanical and entomological origin. Hence, the objective of this study was to screen the in vitro antibacterial activity of honey based on botanical origin produced in Ethiopia. Methods Botanical origin was identi ed by the Melisopalynological analysis method. The inhibition zone was determined by agar well diffusion assay. Minimum inhibitory concentration was done by broth diffusion. Result Six different mono oral honey namely: Guizotia (75.3% pollen grain counted), Coffee arabica (68.6%), Vernonia (90.5%), Sche era abyssinica (100%), Croton macrostachyus (64.4%) and Eucalyptus (100%) honey was harvested through December, February, February, April, May, and June respectively from the study area. All the mono oral honey inhibited the growth of all the bacterial strains even though the diameter of the inhibition zone varied. C. macrostachyus, Vernonia, Guizotia, and C. arabica were comparatively more whilst S. abyssinica was the least effective against all the tested bacterial strains. The grand mean inhibition zone ranged from 17.0 ± 1 mm by C. macrostachyus to 10.5 ± 1 mm by S. abyssinica against all the bacterial strains. Besides, the MIC of botanical honey type ranged from 11.7 ± 3.3% w/v by C. macrostachyus against S. aureus to 37.5 ± 1% w/v by S. abyssinica against P. aeruginosa. Conclusion The current result indicated that there is a disparity in antibacterial properties between mono oral honey. The more effective honey against the tested bacterial strain were those produced from the nectar of Ethiopian medicinal plants; based on literatures. Therefore, further invivo and in-vitro research would be expected from the concerned researchers on the association between the biological compounds present in each part of medicinal plants and honey samples.

Water, 2019

With increasing population, the need for research ideas on the field of reducing wastage of water... more With increasing population, the need for research ideas on the field of reducing wastage of water can save a big amount of water, money, time, and energy. Water leakage (WL) is an essential problem in the field of water supply field. This research is focused on real water loss in the water distribution system located in Ethiopia. Top-down and bursts and background estimates (BABE) methodology is performed to assess the data and the calibration process of the WL variables. The top-down method assists to quantify the water loss by the record and observation throughout the distribution network. In addition, the BABE approach gives a specific water leakage and burst information. The geometrical mean method is used to forecast the population up to 2023 along with their fiscal value by the uniform tariff method. With respect to the revenue lost, 42575 Br and 42664 Br or in 1562$ and 1566$ were lost in 2017 and 2018, respectively. The next five-year population was forecasted to estimate th...

International Journal of Food Properties, Jan 4, 2022

International Journal of Forestry Research

Beekeeping has significantly contributed to environmental conservation and the preservation of na... more Beekeeping has significantly contributed to environmental conservation and the preservation of natural resources. Although the quality and quantity of flora available play a major role in the success of the sector, the botanical makeup of natural vegetation varies greatly. This study was conducted targeting the identification and documentation of major honeybee floras and their flowering calendar. Midland and lowland agroecologies were purposively selected. Continuous field plant registration was performed. Melissopalynological analysis from bee pollen and honey were used to identify floral origin. Field observations identified 59 and 63 plants in the midland and lowlands, respectively. Season 1 had the highest pollen yields, ranging from 11051.8 ± 56.4 g (midlands) to 878.3 ± 18.3 g (lowlands), while season 4 ranged from 16.8 ± 6.3 g (midlands) to 15.6 ± 7.4 g (lowlands) and had the lowest pollen yield. In both regions, February, March, July, and August are the months when pollen i...

International Journal of Food Science

The antioxidant properties of natural honey primarily rely on the floral origin from which nectar... more The antioxidant properties of natural honey primarily rely on the floral origin from which nectar is collected by bees. Thus, the current activity evaluated the antioxidant properties of honey based on its floral type. The honey floral origin was verified by the melissopalynological technique. Antioxidant properties were determined by using standard procedures and analyzed by SAS. Six unifloral honey types with their harvesting month were identified. Accordingly, Guizotia (74% of pollen frequency), Coffea arabica (68%), Vernonia (90%), Croton macrostachyus (64%), Schefflera abyssinica (100%), and Eucalyptus (100%) were cropped in November, February, February, May, April, and June separately. Statistically, a variation ( p < 0.05 ) in antioxidant parameters was displayed between unifloral honeys. Vernonia honey exhibited the maximum total phenol ( 77.2 ± 0.7 ), total flavonoid ( 65.0 ± 3.8 ), and total antioxidant content ( 65.4 ± 0.3 ). On the other hand, S. abyssinica honey reco...

Background Honey is a natural sweetener and viscous food which is synthesized from the nectar of ... more Background Honey is a natural sweetener and viscous food which is synthesized from the nectar of flowering plants by bees. As a worldwide, it has been traditionally used for incurring different diseases. However, In recent times, the emergence of multi and extended drug-resistant human pathogens called for an urgent search for more effective sources of natural products to treat infectious diseases. The biological activity of honey primarily relies on its botanical and entomological origin. Hence, the objective of this study was to screen the in vitro antibacterial activity of honey based on botanical origin produced in Ethiopia. Methods Botanical origin was identified by the Melisopalynological analysis method. The inhibition zone was determined by agar well diffusion assay. Minimum inhibitory concentration was done by broth diffusion. Result Six different monofloral honey namely: Guizotia (75.3% pollen grain counted), Coffee arabica (68.6%), Vernonia (90.5%), Schefflera abyssinica ...

Journal of Biology, Agriculture and Healthcare, 2019

International Journal of Food Properties, 2022