Patricia Castillo-Briceno - Academia.edu (original) (raw)

Papers by Patricia Castillo-Briceno

Esta propuesta de integración de Ciencias, Artes y Comunidad responde a la necesidad de cambio de... more Esta propuesta de integración de Ciencias, Artes y Comunidad responde a la necesidad de cambio de los paradigmas que (i) separan artes de ciencias con una priorización arbitraria de sus diferencias y, (ii) que hacen de artes y ciencias actividades exclusivas. Este distanciamiento entre Ciencias, Artes y Comunidad repercute en sus procesos de enseñanza y ejercicio profesional, y limita su accionar y desarrollo. Por otro lado, el análisis de sus mecanismos de funcionamiento, revela que las ciencias y las artes tienen un eje común en la creatividad y la búsqueda de soluciones. Esta propuesta, desarrollada en Ecuador, explora estos aspectos considerando que las ciencias y las artes son procesos cíclicos de aplicación y generación de conocimiento (racional, empírico y emocional) que evolucionan con la integración y contacto con la comunidad. Para ello se realizaron ciclos de conferencias participativas con el tema “El arte de las Ciencias – La ciencia de las Artes”. Actividad concebida c...

9th International Congress on the Biology of Fish (American Fisheries Society). Barcelona, Spain.... more 9th International Congress on the Biology of Fish (American Fisheries Society). Barcelona, Spain. 2010 Patricia Castillo-Briceño*^, Richard W Farndale+, Alfonsa García-Ayala*, Victoriano Mulero*¨. *Department of Cell Biology and Histology, Faculty of Biology, University of Murcia, Spain + Department of Biochemistry, University of Cambridge, UK. ^ pcasbri@um.es ; ¨ vmulero@um.es Collagen (COLs) specific sequences to which mammalian cells can attach, either directly or through protein intermediaries, has been identified using Toolkits of triple-helical peptides and GXX’GEX motifs within the COLs. We have previously reported that COL1 was able to prime in vitro the respiratory burst and induce a specific set of immune- and ECM-related molecules in phagocytes of the teleost gilthead seabream (Sparus aurata L.). In the present study, we used the seabream fibroblast cell line SAF-1 to identify the binding COL motifs by end-point and real-time cell adhesion assays against the Toolkits of C...

Molecular Immunology

Sex hormones, both estrogens and androgens, have a strong impact on immunity in mammals. In fish,... more Sex hormones, both estrogens and androgens, have a strong impact on immunity in mammals. In fish, the role of androgens in immunity has received little attention and contradictory conclusions have been obtained. However, it is well known that sex steroids are involved in fish growth, osmoregulation and gonad remodelation. In this study, we examine the in vitro effects of testosterone and 11-ketotestosterone, the two main fish androgens, on the professional phagocytes of the teleost fish gilthead seabream (Sparus aurata L.). Although both testosterone and 11-ketotestosterone failed to modulate the respiratory burst of seabream phagocytes, testosterone but not 11-ketotestosterone was able to increase the phagocytic ability of non-activated phagocytes. Curiously, 11-ketotestosterone was more powerful than testosterone at inducing the expression of its own receptor, namely androgen receptor b (ARb), in acidophilic granulocytes (AGs), but none of them affected the basal ARb expression le...

ABSTRACT This work studies the extracellular matrix (ECM) environment and their implications in t... more ABSTRACT This work studies the extracellular matrix (ECM) environment and their implications in the immune response and spermatogenesis in gilthead seabream. This is a useful model to study interactions between immune and reproductive systems, considering its biological characteristics, such as seasonal breeding, testicular involution and sexual reversion. The results showed collagen and its derived peptides as able to act as damage signals (DAMPs), modulating in a specific form the innate immune response and the early inflammation processes in seabream professional phagocytes and fibroblasts. In addition, the strong correlations between ECM related molecules, under physiological conditions and during development, are modulated as part of the response against DAMPs and pathogen derived molecules. Finally, we found that in addition to the integrin beta 1a (ITGB1a) which is constitutively expressed, in seabream there is an ITGB1b which is mainly expressed in testis and during spermato...

Developmental and comparative immunology

Extracellular matrix (ECM) components, in addition to their structural functions, interact with c... more Extracellular matrix (ECM) components, in addition to their structural functions, interact with cell surface receptors and intracellular components to modulate the transduction of signals for cell growth, differentiation, migration, proliferation, polarization, apoptosis and inflammation. Our previous findings in the gilthead seabream (Sparus aurata L.), a marine seasonal hermaphrodite teleost fish, have shown that both endocrine and immune stimuli modulate the expression of matrix metalloproteases (MMPs) and tissue inhibitors of MMP (TIMPs). In addition, collagen type I (COL1) induces the expression of some pro-inflammatory cytokines and MMPs in professional phagocytes. Consequently, in this study we use real-time RT-PCR to analyze the gene expression profile of several ECM-related molecules (MMP-2, -9 and -13, TIMP-2a, and -2b, COL1A1, and integrin beta1a) in different organs of adult specimens as well as in response to innate immune challenges. Our results showed that liver had t...

El Niño - Southern Oscillation and health status of galapagos marine iguana (Amblyrhynchus crista... more El Niño - Southern Oscillation and health status of galapagos marine iguana (Amblyrhynchus cristatus). XXVII National Meeting of Biology. Biology Society of Ecuador. Quito, Ecuador. 2003. Patricia Castillo-Briceño, Cruz Márquez, David Wiedenfeld, Howard Snell y Alberto Jaramillo Fundación Charles Darwin pcastillo@fcdarwin.org.ec; marquez@fcdarwin.org.ec; dwiedenfeld@fcdarwin.org.ec; howard@fcdarwin.org.ec; albertoj@fcdarwin.org.ec Puerto Ayora, Santa Cruz, Galápagos Casilla Postal 17-01-3891 Resumen Es conocido que el Evento de El Niño / Oscilación del Sur (ENOS) es un fenómeno natural de interacción oceánico – atmosférica que ocurre en la región del océano Pacífico tropical; se caracteriza por cambios ambientales típicos que también afectan al componente biológico. En el caso específico de las iguanas marinas (Amblyrhynchus cristatus), estudios científicos han sugerido que este evento tiene un impacto negativo sobre esta especie en las Islas Galápagos (Wikelski, 1997, 2001). La Fun...

El Niño / Southern Oscillation (ENSO) affects biological components by its oceanic- atmospheric i... more El Niño / Southern Oscillation (ENSO) affects biological components by its oceanic- atmospheric interaction in the tropical Pacific ocean. For Galápagos marine iguanas (Amblyrhynchus cristatus Bell, 1825) scientific studies propose a negative impact. Charles Darwin Foundation, in order to acquire long term data, has established a permanent monitoring project in an area of Santa Cruz Island. This area has 6.1 km of coastal land (61 sampling stations), from Punta Núñez (SE of the island) to Charles Darwin Research Station dock. This research shows the health state of the marine iguana population from this zone, considering six levels from 0 to 5 (healthy to dead), comparing ENSO (1997-98) and Post ENSO (2000-01) periods. Their differences and relation to health levels and environmental conditions (rain, superficial sea temperature and air temperature) were determined. Results showed significant differences (z0.05=0.4477; P

Proyecto de investigación:, 2010

Page 1. UNIVERSIDAD DE MURCIA PROGRAMA OFICIAL DE DOCTORADO EN INTEGRACIÓN Y MODULACIÓN DE SEÑALE... more Page 1. UNIVERSIDAD DE MURCIA PROGRAMA OFICIAL DE DOCTORADO EN INTEGRACIÓN Y MODULACIÓN DE SEÑALES EN BIOMEDICINA FACULTAD DE MEDICINA DEPARTAMENTO DE BIOLOGÍA CELULAR E HISTOLOGÍA FACULTAD DE BIOLOGÍA ...

Evo-Devo Meets Marine Ecology: New Frontiers in Ocean Science through Integrative Biology. EMBO W... more Evo-Devo Meets Marine Ecology: New Frontiers in Ocean Science through Integrative Biology. EMBO Workshop. Sant’Angelo d’Ischia, Italy. 2009. Patricia Castillo-Briceño*, Isabel Cabas, Sergio Liarte, José Meseguer, Alfonsa García-Ayala, Victoriano Mulero Department of Cell Biology and Histology, Faculty of Biology, University of Murcia, 30100, Spain. *pcasbri@um.es The gilthead seabream (Sparus aurata L., Teleostei) is a protandrous hermaphrodite, seasonal breeding sparid fish that undergoes sex change during the second or third year of life, depending on the natural environment. The aquaculture of this specie is an important industry widely spread in the Mediterranean area. Our group is especially interested in study the immune response in this bony fish, emphasizing in the processes and molecules involved. It was described that some matrix metalloproteinases (MMPs), such as MMP-2, 3, 9, 13 and 25, are involved in the immune response in mammals. Its expression can be modulated in the...

Molecular Immunology, 2008

The testicular cystic structure and the abrupt morphological changes that the fish testis undergo... more The testicular cystic structure and the abrupt morphological changes that the fish testis undergoes during the reproductive cycle (RC) make it an interesting model for studying the regulation of spermatogenesis, in particular the role of matrix metalloproteinases (Mmps). The gilthead seabream is a seasonal breeding teleost whose testis undergoes drastic remodeling events, especially during the post-spawning stage when a massive infiltration of a immune cell type, the acidophilic granulocytes, occurred. Bearing this in mind, we studied the gilthead seabream testis gelatinolytic activities involved in migration and tissue remodeling and its regulation by endocrine, immune and tissue stimuli. Thus, we demonstrated that the germinal epithelium of the testis showed gelatinolytic activity during spermatogenesis and post-spawning but not during resting, when only scarce interstitial cells were stained. Moreover, the precursor and mature forms of two gelatinases, Mmp2- and Mmp9-like, were active in the gonad, whose activities were up-regulated by 17beta-estradiol (E(2)) but not by lipopolysaccharide (LPS)/bacterial DNA (VaDNA) in testicular cell suspensions. E(2) and LPS/VaDNA also up-regulated a variety of cytokines and chemokines. We also cloned mmp9, mmp13, tissue inhibitors of Mmps (timp)-2a and timp2b genes and found that all of them were expressed in the gonad in a RC stage-dependent manner. Interestingly, mmps and timps were highly expressed by the testicular acidophilic granulocytes. Moreover, in these cells, the gelatinolytic activity seemed to correspond to the precursor and mature forms of putative Mmp2 and Mmp9 gelatinases, while the main gelatinolytic activity seemed to correspond to the mature form of Mmp2 in head-kidney acidophilic granulocytes. Finally, although none of the stimuli used were able to induce the gelatinolytic activity of Mmp9-like in head-kidney acidophilic granulocytes, the expression of mmp9, timp2a and timp2b were all up-regulated by LPS/VaDNA in these cells, while only mmp9 and timp2a expression increased upon stimulation with gelatin.

Molecular Immunology

Molecular Immunology, 2009

The innate immune system mediates the initial inflammatory response that follows infection or inj... more The innate immune system mediates the initial inflammatory response that follows infection or injury. Although the innate immune response of fish to infection has been relatively well characterized during recent years at both cellular and molecular levels, no studies have examined the role of extracellular matrix (ECM) in the regulation of innate immunity and inflammation. We report here that collagen and gelatin in vitro were able to prime the respiratory burst of phagocytes from the bony fish gilthead seabream. In addition, collagen and gelatin induced a specific set of immune-related and ECM remodelling enzymes that substantially differed from that induced by pathogen-associated molecular patterns. Notably, both collagen and gelatin induced the expression of interleukin-1 , chemokine (C-C motif) ligand 4 and matrix metalopeptidases (MMP) 9 and 13 in acidophilic granulocytes and macrophages but were unable to significantly increase the expression of other pro-inflammatory genes. Furthermore, it was found that the MMP2/MMP9 inhibitor V had a dose-dependent inhibitory effect on seabream phagocyte activation by either collagen or gelatin. In contrast, pre-treatment of collagen and gelatin by collagenase resulted in a higher stimulatory capacity compared to non-digested proteins.

Molecular Immunology, 2011

Specific sites and sequences in collagen to which cells can attach, either directly or through pr... more Specific sites and sequences in collagen to which cells can attach, either directly or through protein intermediaries, were identified using Toolkits of 63-amino acid triple-helical peptides and specific shorter GXX&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;#39;GEX″ motifs, which have different intrinsic affinity for integrins that mediate cell adhesion and migration. We have previously reported that collagen type I (COL-I) was able to prime in vitro the respiratory burst and induce a specific set of immune- and extracellular matrix-related molecules in phagocytes of the teleost fish gilthead seabream (Sparus aurata L.). It was also suggested that COL-I would provide an intermediate signal during the early inflammatory response in gilthead seabream. Since fibroblasts are highly involved in the initiation of wound repair and regeneration processes, in the present study SAF-1 cells (gilthead seabream fibroblasts) were used to identify the binding motifs in collagen by end-point and real-time cell adhesion assays using the collagen peptides and Toolkits. We identified the collagen motifs involved in the early magnesium-dependent adhesion of these cells. Furthermore, we found that peptides containing the GFOGER and GLOGEN motifs (where O is hydroxyproline) present high affinity for SAF-1 adhesion, expressed as both cell number and surface covering, while in cell suspensions, these motifs were also able to induce the expression of the genes encoding the proinflammatory molecules interleukin-1β and cyclooxygenase-2. These data suggest that specific collagen motifs are involved in the regulation of the inflammatory and healing responses of teleost fish.

Frontiers in zoology, 2014

Background: How important are sexual hormones beyond their function in reproductive biology has y... more Background: How important are sexual hormones beyond their function in reproductive biology has yet to be understood. In this study, we analyzed the effects of sex steroids on the biology of the embryonic amphibian epidermis, which represents an easily amenable model of non-reproductive mucociliary epithelia (MCE). MCE are integrated systems formed by multiciliated (MC), mucus-secreting (MS) and mitochondrion-rich (MR) cell populations that are shaped by their microenvironment. Therefore, MCE could be considered as ecosystems at the cellular scale, found in a wide array of contexts from mussel gills to mammalian oviduct. Results: We showed that the natural estrogen (estradiol, E2) and androgen (testosterone, T) as well as the synthetic estrogen (ethinyl-estradiol, EE2), all induced a significant enhancement of MC cell numbers. The effect of E2, T and EE2 extended to the MS and MR cell populations, to varying degrees. They also modified the expression profile of RNA MCE markers, and induced a range of "non-typical" cellular phenotypes, with mixed identities and aberrant morphologies, as revealed by imaging analysis through biomarker confocal detection and scanning electron microscopy. Finally, these hormones also affected tadpole pigmentation, revealing an effect on the entire cellular ecosystem of the Xenopus embryonic skin.

Molecular Immunology, 2011

Specific sites and sequences in collagen to which cells can attach, either directly or through pr... more Specific sites and sequences in collagen to which cells can attach, either directly or through protein intermediaries, were identified using Toolkits of 63-amino acid triple-helical peptides and specific shorter GXX GEX motifs, which have different intrinsic affinity for integrins that mediate cell adhesion and migration. We have previously reported that collagen type I (COL-I) was able to prime in vitro the respiratory burst and induce a specific set of immune-and extracellular matrix-related molecules in phagocytes of the teleost fish gilthead seabream (Sparus aurata L.). It was also suggested that COL-I would provide an intermediate signal during the early inflammatory response in gilthead seabream. Since fibroblasts are highly involved in the initiation of wound repair and regeneration processes, in the present study SAF-1 cells (gilthead seabream fibroblasts) were used to identify the binding motifs in collagen by end-point and real-time cell adhesion assays using the collagen peptides and Toolkits. We identified the collagen motifs involved in the early magnesium-dependent adhesion of these cells. Furthermore, we found that peptides containing the GFOGER and GLOGEN motifs (where O is hydroxyproline) present high affinity for SAF-1 adhesion, expressed as both cell number and surface covering, while in cell suspensions, these motifs were also able to induce the expression of the genes encoding the proinflammatory molecules interleukin-1␤ and cyclooxygenase-2. These data suggest that specific collagen motifs are involved in the regulation of the inflammatory and healing responses of teleost fish.

Steroids, 2013

Current knowledge on the sensitivity of marine fish to androgenic environmental chemicals is limi... more Current knowledge on the sensitivity of marine fish to androgenic environmental chemicals is limited, despite the growing interest in the effects of endocrine disrupting chemicals. To study in vivo the effects of testosterone (T) on the fish immune response, we used a microencapsulation implant technique, the in situ forming microparticle system, containing 1 mg T/kg body weight (T-ISM), in adult specimens of gilthead seabream (Sparus aurata L.), a species of great economic interest. We demonstrated that implants themselves (without T) have no significant effect on most of the parameters measured. In T-ISM implanted fish, T serum levels reached supraphysiological concentrations accompanied by a slight increase in 11-ketotestosterone and 17b-estradiol levels 21 days post-implantation (dpi). Liver and head-kidney samples were processed 7 and 21 dpi to assess T-ISM effect on (i) the mRNA expression of genes involved in the metabolism of steroid hormones and in the immune response, and (ii) phagocyte activities. The expression profile of cytokines, chemokines and immune receptors was altered in T-ISM implanted animals that showed an early pro-inflammatory tendency, and then, a mixed pro-/anti-inflammatory activation during longer exposure. Furthermore, the enhancement of phagocytic activity and the production of reactive oxygen species by leukocytes 21 dpi in T-ISM implanted specimens suggest fine modulation of the innate immune response by T. Taken together, these data demonstrate for the first time the feasibility of using ISM implants in an aquatic species, and provide new data on the role played by T on the immune response in fish.

Molecular Immunology

Developmental and comparative immunology

Proyecto de investigación:, 2010

Molecular Immunology, 2008

Molecular Immunology

Molecular Immunology, 2009

Molecular Immunology, 2011

Frontiers in zoology, 2014

Molecular Immunology, 2011

Specific sites and sequences in collagen to which cells can attach, either directly or through pr... more Specific sites and sequences in collagen to which cells can attach, either directly or through protein intermediaries, were identified using Toolkits of 63-amino acid triple-helical peptides and specific shorter GXX GEX motifs, which have different intrinsic affinity for integrins that mediate cell adhesion and migration. We have previously reported that collagen type I (COL-I) was able to prime in vitro the respiratory burst and induce a specific set of immune-and extracellular matrix-related molecules in phagocytes of the teleost fish gilthead seabream (Sparus aurata L.). It was also suggested that COL-I would provide an intermediate signal during the early inflammatory response in gilthead seabream. Since fibroblasts are highly involved in the initiation of wound repair and regeneration processes, in the present study SAF-1 cells (gilthead seabream fibroblasts) were used to identify the binding motifs in collagen by end-point and real-time cell adhesion assays using the collagen peptides and Toolkits. We identified the collagen motifs involved in the early magnesium-dependent adhesion of these cells. Furthermore, we found that peptides containing the GFOGER and GLOGEN motifs (where O is hydroxyproline) present high affinity for SAF-1 adhesion, expressed as both cell number and surface covering, while in cell suspensions, these motifs were also able to induce the expression of the genes encoding the proinflammatory molecules interleukin-1␤ and cyclooxygenase-2. These data suggest that specific collagen motifs are involved in the regulation of the inflammatory and healing responses of teleost fish.

Steroids, 2013