Satya Parida - Academia.edu (original) (raw)

Papers by Satya Parida

Research paper thumbnail of Prediction and characterisation of novel epitopes of serotype A FMD viruses circulating in East Africa using site-directed mutagenesis

The Journal of general virology, Jan 22, 2015

Epitopes on the surface of the foot-and-mouth disease virus (FMDV) capsid have been identified by... more Epitopes on the surface of the foot-and-mouth disease virus (FMDV) capsid have been identified by monoclonal antibody (mAb) escape mutant studies leading to the designation of four antigenic sites in serotype A FMDV. Previous work focused on viruses isolated mainly from Asia, Europe and Latin America. In this study we report prediction of epitopes in African serotype A FMDVs and tested selected epitopes using reverse genetics. Twenty-four capsid amino acid residues were predicted to be of antigenic significance by analyzing the capsid sequences (n=56) using in-silico methods and six residues by correlating capsid sequence with serum-virus neutralization data. The predicted residues were distributed on the surface-exposed capsid regions, VP1-VP3. The significance of residue changes at eight of the predicted epitopes was tested by site directed mutagenesis using a cDNA clone resulting in the generation of 12 mutant viruses involving seven sites. The effect of the amino acid substituti...

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Research paper thumbnail of Bovine serum panel for evaluating foot-and-mouth disease virus nonstructural protein antibody tests

Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc, 2007

A panel of 36 sera has been assembled from experimental cattle that had been infected by inoculat... more A panel of 36 sera has been assembled from experimental cattle that had been infected by inoculation or contact exposure with 4 serotypes of foot-and-mouth disease virus (FMDV) with or without prior vaccination. Virus replication and persistence had been characterized in all of the animals. The proportion of the sera scored positive by 5 tests for antibodies to the nonstructural proteins of FMDV varied, suggesting that the panel can discriminate between the sensitivity with which such tests are able to identify infected cattle. Use of this panel will help in assessment of new tests and quality control of existing methods.

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Research paper thumbnail of Transmission of foot-and-mouth disease virus from experimentally infected Indian buffalo (Bubalus bubalis) to in-contact naïve and vaccinated Indian buffalo and cattle

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Research paper thumbnail of Abstract withdrawn

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Research paper thumbnail of Further evaluation of higher potency vaccines for early protection of cattle against FMDV direct contact challenge

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Research paper thumbnail of Use of a Standardized Bovine Serum Panel To Evaluate a Multiplexed Nonstructural Protein Antibody Assay for Serological Surveillance of Foot-and-Mouth Disease

Clinical and Vaccine Immunology, 2007

Liquid array technology has previously been used to show proof of principle of a multiplexed nons... more Liquid array technology has previously been used to show proof of principle of a multiplexed nonstructural protein serological assay to differentiate foot-and-mouth disease virus-infected and vaccinated animals. The current multiplexed assay consists of synthetically produced peptide signatures 3A, 3B, and 3D and the recombinant protein signature 3ABC in combination with four controls. To determine the diagnostic specificity of each signature

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Research paper thumbnail of Sequence analysis of the phosphoprotein gene of peste des petits ruminants (PPR) virus: editing of the gene transcript

Virus Research, 2003

The gene encoding the phosphoprotein of the vaccine strain of Peste des petits ruminants (PPR) vi... more The gene encoding the phosphoprotein of the vaccine strain of Peste des petits ruminants (PPR) virus (Nigeria 75/1 vaccine strain) has been cloned and its nucleotide sequence been determined. This gene is 1655 nucleotides long and encodes two overlapping open reading frames (ORFs). Translation from the first AUG would produce a polypeptide of 509 amino acid residues with a predicted

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Research paper thumbnail of Characteristics of a foot-and-mouth disease virus with a partial VP1 G-H loop deletion in experimentally infected cattle

Veterinary Microbiology, 2014

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Research paper thumbnail of Molecular characterization of peste-des-petits ruminants virus (PPRV) isolated from an outbreak in the Indo-Bangladesh border of Tripura state of North-East India

Veterinary microbiology, Jan 5, 2014

Peste-des-petits- ruminants (PPR) is a highly contagious and devastating disease of goats and she... more Peste-des-petits- ruminants (PPR) is a highly contagious and devastating disease of goats and sheep. Although India is endemic for PPR, Tripura, a state in North East India has never been reported confirmed PPR outbreaks. Recently, an outbreak of PPR occurred in non-descript goats at the Sabroom town of Tripura state in North-East India in June, 2013. The causative agent, PPR virus (PPRV) was confirmed by sandwich ELISA, virus isolation and N gene based RT-PCR and sequencing. The sequence and phylogenetic analysis confirmed the involvement of lineage IV PPR virus in the outbreak. The outbreak viruses from Tripura state were clustered mainly with circulating viruses from Bangladesh, India, China, Pakistan, Tajikistan, Dubai and Kurdistan. However, the nucleotide sequence homology ranged from 99.2 to 99.6% with the PPR strains circulating in Bangladesh during 2011 and 2012 whereas 95.5-98% homology has been observed with the viruses from India and other countries. These findings sugge...

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Research paper thumbnail of Emergence of Lineage IV Peste des Petits Ruminants Virus in Ethiopia: Complete Genome Sequence of an Ethiopian Isolate 2010

Transboundary and Emerging Diseases, 2014

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Research paper thumbnail of Development of a competitive ELISA for the detection of antibodies against the 3B protein of foot-and-mouth disease virus

Clinical and Vaccine Immunology, 2015

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Research paper thumbnail of Marker vaccines and the impact of their use on diagnosis and prophylactic measures

Summary Molecular biology and technical advances in DNA recombination have ushered in a new era i... more Summary Molecular biology and technical advances in DNA recombination have ushered in a new era in vaccinology. This article examines the recent development of specific marker vaccines and examines the impact of their use on the diagnosis and prevention of major infectious diseases. Gene-deleted vaccines, DIVA strategies (differentiating infected from vaccinated animals) and similar methods have been successfully applied in

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Research paper thumbnail of Rinderpest virus lineage differentiation using RT-PCR and SNAP-ELISA

Journal of Virological Methods, 2003

An RT-PCR/ELISA system has been developed that detects and differentiates Rinderpest virus (RPV) ... more An RT-PCR/ELISA system has been developed that detects and differentiates Rinderpest virus (RPV) from the other closely related morbillivirus of ruminants, Peste des petits Ruminants virus (PPRV). In addition, using lineage specific probes, it is possible to determine whether the virus sample is wild-type or vaccine, and the likely origin of the outbreak if it is wild-type. It involves carrying

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Research paper thumbnail of Genetic and antigenic characterisation of serotype A FMD viruses from East Africa to select new vaccine strains

Vaccine, 2014

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Research paper thumbnail of Matrix protein and glycoproteins F and H of Peste-des-petits-ruminants virus function better as a homologous complex

Journal of General Virology, 2006

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Research paper thumbnail of Toll-Like Receptor Responses to Peste des petits ruminants Virus in Goats and Water Buffalo

PLoS ONE, 2014

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Research paper thumbnail of Importance of the extracellular and cytoplasmic/transmembrane domains of the haemagglutinin protein of rinderpest virus for recovery of viable virus from cDNA copies

Virus Research, 2006

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Research paper thumbnail of Utility of automated real-time RT-PCR for the detection of foot-and-mouth disease virus excreted in milk

Veterinary Research, 2006

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Research paper thumbnail of Morbillivirus vaccines: Recent successes and future hopes

Vaccine, 2014

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Research paper thumbnail of Further evaluation of higher potency vaccines for early protection of cattle against FMDV direct contact challenge

Vaccine, 2007

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Research paper thumbnail of Prediction and characterisation of novel epitopes of serotype A FMD viruses circulating in East Africa using site-directed mutagenesis

The Journal of general virology, Jan 22, 2015

Epitopes on the surface of the foot-and-mouth disease virus (FMDV) capsid have been identified by... more Epitopes on the surface of the foot-and-mouth disease virus (FMDV) capsid have been identified by monoclonal antibody (mAb) escape mutant studies leading to the designation of four antigenic sites in serotype A FMDV. Previous work focused on viruses isolated mainly from Asia, Europe and Latin America. In this study we report prediction of epitopes in African serotype A FMDVs and tested selected epitopes using reverse genetics. Twenty-four capsid amino acid residues were predicted to be of antigenic significance by analyzing the capsid sequences (n=56) using in-silico methods and six residues by correlating capsid sequence with serum-virus neutralization data. The predicted residues were distributed on the surface-exposed capsid regions, VP1-VP3. The significance of residue changes at eight of the predicted epitopes was tested by site directed mutagenesis using a cDNA clone resulting in the generation of 12 mutant viruses involving seven sites. The effect of the amino acid substituti...

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Bovine serum panel for evaluating foot-and-mouth disease virus nonstructural protein antibody tests

Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc, 2007

A panel of 36 sera has been assembled from experimental cattle that had been infected by inoculat... more A panel of 36 sera has been assembled from experimental cattle that had been infected by inoculation or contact exposure with 4 serotypes of foot-and-mouth disease virus (FMDV) with or without prior vaccination. Virus replication and persistence had been characterized in all of the animals. The proportion of the sera scored positive by 5 tests for antibodies to the nonstructural proteins of FMDV varied, suggesting that the panel can discriminate between the sensitivity with which such tests are able to identify infected cattle. Use of this panel will help in assessment of new tests and quality control of existing methods.

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Research paper thumbnail of Transmission of foot-and-mouth disease virus from experimentally infected Indian buffalo (Bubalus bubalis) to in-contact naïve and vaccinated Indian buffalo and cattle

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Abstract withdrawn

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Further evaluation of higher potency vaccines for early protection of cattle against FMDV direct contact challenge

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Use of a Standardized Bovine Serum Panel To Evaluate a Multiplexed Nonstructural Protein Antibody Assay for Serological Surveillance of Foot-and-Mouth Disease

Clinical and Vaccine Immunology, 2007

Liquid array technology has previously been used to show proof of principle of a multiplexed nons... more Liquid array technology has previously been used to show proof of principle of a multiplexed nonstructural protein serological assay to differentiate foot-and-mouth disease virus-infected and vaccinated animals. The current multiplexed assay consists of synthetically produced peptide signatures 3A, 3B, and 3D and the recombinant protein signature 3ABC in combination with four controls. To determine the diagnostic specificity of each signature

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Sequence analysis of the phosphoprotein gene of peste des petits ruminants (PPR) virus: editing of the gene transcript

Virus Research, 2003

The gene encoding the phosphoprotein of the vaccine strain of Peste des petits ruminants (PPR) vi... more The gene encoding the phosphoprotein of the vaccine strain of Peste des petits ruminants (PPR) virus (Nigeria 75/1 vaccine strain) has been cloned and its nucleotide sequence been determined. This gene is 1655 nucleotides long and encodes two overlapping open reading frames (ORFs). Translation from the first AUG would produce a polypeptide of 509 amino acid residues with a predicted

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Characteristics of a foot-and-mouth disease virus with a partial VP1 G-H loop deletion in experimentally infected cattle

Veterinary Microbiology, 2014

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Molecular characterization of peste-des-petits ruminants virus (PPRV) isolated from an outbreak in the Indo-Bangladesh border of Tripura state of North-East India

Veterinary microbiology, Jan 5, 2014

Peste-des-petits- ruminants (PPR) is a highly contagious and devastating disease of goats and she... more Peste-des-petits- ruminants (PPR) is a highly contagious and devastating disease of goats and sheep. Although India is endemic for PPR, Tripura, a state in North East India has never been reported confirmed PPR outbreaks. Recently, an outbreak of PPR occurred in non-descript goats at the Sabroom town of Tripura state in North-East India in June, 2013. The causative agent, PPR virus (PPRV) was confirmed by sandwich ELISA, virus isolation and N gene based RT-PCR and sequencing. The sequence and phylogenetic analysis confirmed the involvement of lineage IV PPR virus in the outbreak. The outbreak viruses from Tripura state were clustered mainly with circulating viruses from Bangladesh, India, China, Pakistan, Tajikistan, Dubai and Kurdistan. However, the nucleotide sequence homology ranged from 99.2 to 99.6% with the PPR strains circulating in Bangladesh during 2011 and 2012 whereas 95.5-98% homology has been observed with the viruses from India and other countries. These findings sugge...

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Research paper thumbnail of Emergence of Lineage IV Peste des Petits Ruminants Virus in Ethiopia: Complete Genome Sequence of an Ethiopian Isolate 2010

Transboundary and Emerging Diseases, 2014

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Development of a competitive ELISA for the detection of antibodies against the 3B protein of foot-and-mouth disease virus

Clinical and Vaccine Immunology, 2015

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Marker vaccines and the impact of their use on diagnosis and prophylactic measures

Summary Molecular biology and technical advances in DNA recombination have ushered in a new era i... more Summary Molecular biology and technical advances in DNA recombination have ushered in a new era in vaccinology. This article examines the recent development of specific marker vaccines and examines the impact of their use on the diagnosis and prevention of major infectious diseases. Gene-deleted vaccines, DIVA strategies (differentiating infected from vaccinated animals) and similar methods have been successfully applied in

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Rinderpest virus lineage differentiation using RT-PCR and SNAP-ELISA

Journal of Virological Methods, 2003

An RT-PCR/ELISA system has been developed that detects and differentiates Rinderpest virus (RPV) ... more An RT-PCR/ELISA system has been developed that detects and differentiates Rinderpest virus (RPV) from the other closely related morbillivirus of ruminants, Peste des petits Ruminants virus (PPRV). In addition, using lineage specific probes, it is possible to determine whether the virus sample is wild-type or vaccine, and the likely origin of the outbreak if it is wild-type. It involves carrying

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Genetic and antigenic characterisation of serotype A FMD viruses from East Africa to select new vaccine strains

Vaccine, 2014

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Matrix protein and glycoproteins F and H of Peste-des-petits-ruminants virus function better as a homologous complex

Journal of General Virology, 2006

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Toll-Like Receptor Responses to Peste des petits ruminants Virus in Goats and Water Buffalo

PLoS ONE, 2014

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Importance of the extracellular and cytoplasmic/transmembrane domains of the haemagglutinin protein of rinderpest virus for recovery of viable virus from cDNA copies

Virus Research, 2006

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Utility of automated real-time RT-PCR for the detection of foot-and-mouth disease virus excreted in milk

Veterinary Research, 2006

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Morbillivirus vaccines: Recent successes and future hopes

Vaccine, 2014

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Further evaluation of higher potency vaccines for early protection of cattle against FMDV direct contact challenge

Vaccine, 2007

Bookmarks Related papers MentionsView impact