Prof. Nurul Absar - Academia.edu (original) (raw)

Papers by Prof. Nurul Absar

Human T-lymphotropic virus (HTLV), a retrovirus belonging to the oncovirus family, has long been ... more Human T-lymphotropic virus (HTLV), a retrovirus belonging to the oncovirus family, has long been linked to be associated with various inflammatory and immunosuppressive disorders. To combat the devastating impact of this virus, our study employed a reverse vaccinology approach to design a multi-epitope-based vaccine targeting the highly virulent subtypes of HTLV. We conducted a comprehensive analysis of the molecular interactions between the vaccine and Toll-like receptors (TLRs), providing valuable insights for future research on preventing and managing HTLV-related diseases and any possible outbreaks. The vaccine was designed by focusing on the envelope glycoprotein gp62, a crucial protein involved in the infectious process and immune mechanisms of HTLV inside the human body. Epitope mapping identified T cell and B cell epitopes with low binding energies, ensuring their immunogenicity and safety. Linkers and adjuvants were incorporated to enhance the vaccine’s stability, antigenic...

Journal of Biomolecular Structure and Dynamics, 2019

Snake venom of Naja naja comprises of several types of enzymes, and among them, water-soluble pro... more Snake venom of Naja naja comprises of several types of enzymes, and among them, water-soluble proteolytic enzyme, phospholipase A2 (PLA 2), is noteworthy for its numerous adverse effects, such as; cytotoxicity, cardiotoxicity, haemolytic, anti-coagulant, and hypotensive effects, including being highly potent as a neurotoxin. Limited anti-venom therapy (with their lower efficacy) has attracted considerable pharmacological interest to develop potent inhibitors of PLA 2. Thus, 34 experimentally proven and diverse synthetic inhibitors of PLA 2 were screened primarily on the basis of Glide extra precision docking and MM-GBSA rescoring function. Then, ten potential hits were subjected to induced fit docking, in which top three potential inhibitors were considered, and those were found to interact with Ca 2+ , disulfide binding site, and phosphatidylcholine activation sites, thereby, possibly disrupting the catalytic activity of Ca 2+ as well as the inflammatory functions of PLA 2. These compounds showed positive remarks on various physiochemical properties and pharmacologically relevant descriptors. Gap energy and thermodynamic properties were investigated by employing density functional theory for all compounds to understand their chemical reactivity and thermodynamic stability. Molecular dynamics simulation was performed for 100ns in order to evaluate the stability and binding modes of docked complexes, and the energy of binding was calculated through MM-PBSA analysis. On the whole, the proposed compounds could be used for targeted inhibition.

This study is aimed to establish the invitro screening of secondary metabolites namely alkaloids,... more This study is aimed to establish the invitro screening of secondary metabolites namely alkaloids, steroids, alcoholic compounds, glycosides, and amide group containing compounds from warm-water extracts of puffer fish dorsal flesh, head, tail and liver tissues by conventional laboratory procedures. In secondary metabolites screening, the warm-water extract of puffer fish liver tissues excepting fish dorsal, head, and tail extract showed positive test of alkaloids, gycosides and alcoholic group test while gave negative test of steroid, amide group containing compounds. This property is found to similar to TTX, which was isolated from puffer fish available in Japan. On the other hand, fish extracts from dorsal, head, and tail showed the positive test against glycosides, alcohol, and amide but not for steroids, and alkaloids. To observe bioactivity, the warm-water crude extracts were prepared at the concentration of 02, 04, 08, 16, 32, and 64 μg/ml, and their bioactivity were tested us...

Journal of Medical Microbiology & Diagnosis, 2014

Background: Hepatitis B virus surface antigen (HBsAg) is used for the detection of Hepatitis B vi... more Background: Hepatitis B virus surface antigen (HBsAg) is used for the detection of Hepatitis B virus (HBV) infection and to predict disease progression. Objectives: The main objective of this study was to observe the pattern of HBV viral load levels among people in terms of age and sex distribution in Bangladesh. Method: Blood specimen was collected from 585 objects with HBsAg Positive and assayed for the quantity of hepatitis B virus using PCR based technique. Results: It is found from the study that the mean viral load was 353,500,000 DNA copies/ml for 20-35 age group, 249,300,000 DNA copies/ml for 35-50 age group and 104,800,000 DNA copies/ml for 50-65 age group. The median HBV viral loads for male and female were 58,494 and 103,287 DNA copies/ml, respectively. Conclusion: High viral load was observed in the 21-35 and 36-50 age group while females are at most risk due to HBV infection as their viral load is higher compared to male.

Pak J Med Sci April, 2007

An intracellular protease was extracted and purified from Pseudomonas aeruginosa by ion-exchange ... more An intracellular protease was extracted and purified from Pseudomonas aeruginosa by ion-exchange chromatography on DEAE-cellulose followed by CMcellulose and rechromatography on DEAE-cellulose. The purified protease was found to be homogeneous as judged by ...

Journal of The Chinese Chemical Society, Oct 1, 2008

Three lectins were isolated from an extract of jute leaves (Chorchorus olitorius) and purified by... more Three lectins were isolated from an extract of jute leaves (Chorchorus olitorius) and purified by gel filtration on Sephadex G-50 of the 100% ammonium sulfate saturated crude extract, followed by ion-exchange chromatography on DEAE-cellulose were designated as JLL-1, JLL-2 and JLL-3. All the lectins were homogeneous as judged by SDS-polyacrylamide slab gel electrophoresis and gave single bands. The molecular weights of the three lectins were estimated by the same method were 35000, 38000 and 42000, respectively. The lectins specifically agglutinated rat red blood cells. The agglutination of JLL-1 was inhibited by D-mannose/D-glucose and their derivatives, whereas D-galactose was found to be the potent inhibitor for the agglutination of JLL-2 and JLL-3. The lectins were glycoprotein in nature with a neutral sugar content of 1.3%, 1.2% and 0.8% for JLL-1, JLL-2 and JLL-3, respectively. The hemagglutinating activity of JLL-2 was also investigated after the treatment of physico-chemical agents. The lectin showed maximum activity between the range of pH 7.2-8.0 and the range of temperature of 20-30°C. The activity of lectin decreased after treatment with a higher concentration of acetic acid and urea. In the presence EDTA the activity was inhibited while the presence of Ca +2 , Mn +2 and K + increased the activity of the lectin moderately.

Bioresources, Jun 7, 2012

Peroxidase catalyzes the oxidation of various electron donor substrates such as phenol and aromat... more Peroxidase catalyzes the oxidation of various electron donor substrates such as phenol and aromatic amines in the presence of hydrogen peroxide. In this study, peroxidase was purified 164-fold from the leaves of Moringa oleifera L. with a recovery of 28% by ammonium sulphate precipitation, DEAE-cellulose column chromatography, Sephadex G-200 column chromatography, and Con-A column chromatography. SDS-PAGE showed a polypeptide band with molecular weight of 43 kDa. The enzyme was found to be a single subunit in nature. The purified enzyme displayed optimum activity at pH 6.0 and at a temperature of 50 °C with a K m value of 0.2335 mM for guaiacol as best substrate. It is a glycoprotein that contains 9.05% sugar as estimated by the phenol sulfuric acid method. Some ions (Ni

Journal of Food Processing and Preservation, Oct 27, 2013

ABSTRACT The integument (high collagen content) of sea cucumber Stichopus vastus is gener-ally wa... more ABSTRACT The integument (high collagen content) of sea cucumber Stichopus vastus is gener-ally wasted after harvesting, whereas only its stomach and intestines are eaten in few Asian countries. Amino acid composition, thermal transition temperature (Tm), zeta potential, solubility, moisture absorption and retention capacities, proximate composition and morphology of pepsin-solubilized collagen (PSC) isolated from the integument of S. vastus were studied. Amino acid composition revealed that glycine was dominant in the isolated collagen. PSC was found to have an isoelectric pH of 4.67, good moisture absorption and retention capacity at higher humidity, a sharp effect of pH and NaCl concentration on solubility, and an inverse relationship between temperature and viscosity. PSC also showed the maximum Tm to be 37.3C, very high protein content and ultrastructural charac-teristics. Hence, the PSC has the potential to be used as a functional ingredient in food, cosmetics and nutraceutical products. PRACTICAL APPLICATIONS Sea cucumber S. vastus is an underutilized resource as most of its parts including integument, which is highly rich in collagen, are discarded as waste except its stomach and intestines, which are consumed as salad. At present, land-based mammals are the main sources of industrial collagen, which has a risk of viral disease transmission and religious disputation, whereas marine sources are free from such criticisms. Besides, collagen isolated from such waste might have indus-trial applications. The purpose of this research is to investigate physicochemical and biochemical properties of PSC isolated from the integument of S. vastus for exploring the potential applications in processing of different industrial products as an alternative to land-based origin.

Journal of The Chinese Chemical Society, Apr 1, 2007

Three lectins designated as SLL-1, SLL-2 and SLL-3 were purified from small sized Sajna (Moringa ... more Three lectins designated as SLL-1, SLL-2 and SLL-3 were purified from small sized Sajna (Moringa oleifera L.) leaves by gel filtration of 100% ammonium sulfate saturated crude protein extract on Sephadex G-75 followed by ion-exchange chromatography on DEAE and affinity chromatography on

Indian journal of plant physiology, 2000

The activities of α-and β-galactosidase, and α-and β-glucosidase in the pulp of 10 mango cultivar... more The activities of α-and β-galactosidase, and α-and β-glucosidase in the pulp of 10 mango cultivars (Fazli, Langra, Khirshapat, Kazlasundori, Kishanbhog, Himsagar, Ranipasand, Gopalbhog, Lata Bombai and Aswina), at 3 ripening stages (immature, mature and ripe), were investigated. ...

Background: Mutations in SMPX gene can disrupt the normal activity of the SMPX protein which is i... more Background: Mutations in SMPX gene can disrupt the normal activity of the SMPX protein which is involved in hearing process. Objective: In this study, deleterious non-synonymous single nucleotide polymorphisms were isolated from the neutral variants by using several bioinformatics tools. Method: Firstly, dbSNP database hosted by NCBI was used to retrieve the SNPs of SMPX gene, secondly, SIFT was used primarily to screen the damaging SNPs. Further, for validation PROVEAN, PredictSNP and PolyPhen 2 were used. I-Mutant 3 was utilized to analyze the protein stability change and MutPred predicted the molecular mechanism of protein stability change. Finally evolutionary conservation was done to study their conservancy by using ConSurf server. Results: A total of 26 missense (0.6517%) and 3 nonsense variants (0.075%) were retrieved and among them 4 mutations were found deleterious by all the tools of this experiment and are also highly conserved according to ConSurf server. rs772775896, rs759552778, rs200892029 and rs1016314772 are the reference IDs of deleterious mutations where the substitutions are S71L, N19D, A29T and K54N. Loss of Ubiquitination, loss of methylation, loss of glycosylation, and loss of MoRF binding motifs are the root causes of protein stability change. Conclusion: This is the first study regarding nsSNPs of SMPX gene where the most damaging SNPs were screened that are associated with the SMPX gene and can be used for further research to study their effect on protein structure and function, their dynamic behavior and how they actually affect protein's flexibility.

International journal of food science, 2013

A detailed study was carried out with the postharvest mangoes (namely, the Langra and the Khirsha... more A detailed study was carried out with the postharvest mangoes (namely, the Langra and the Khirshapat) treated with different levels of Bavistin DF (BDF) solution (namely, 250, 500, and 750 ppm) for obtaining results on biochemical changes as well as storability of postharvest mango. The experiment was laid out in randomized complete block design with three replicates. The results of the experiments exhibited that only the single effect of varieties was found to be significant in most of the parameters studied. The Langra enriched a greater quantity of titratable acidity and total soluble solid (TSS) at 3rd day, over the Khirshapat. On the other hand, Khirshapat showed increased pulp pH and TSS at all the storage duration. The results explored that some physicochemical properties, namely, pulp pH, TSS, sugar (total, reducing, and nonreducing), and titratable acidity along with shelf life drastically decreased from untreated mangoes. Bavistin DF with the doses of 750 ppm showed better results in delaying the changes in physicochemical properties and extended shelf life.

Tropical Journal of Natural Product Research, Feb 4, 2019

biocompatible, economical and easily available, they are best likened to semi-synthetic excipient... more biocompatible, economical and easily available, they are best likened to semi-synthetic excipients because of their less toxicity, low cost, availability, emollient and non-irritant nature. 5,6 The synthetic polymers have certain disadvantages, such as high cost, toxicity, environmental pollution during synthesis, non-renewable sources, side effects, less patient compliance, etc. 7 Tinospora cordifolia (Figure 1) commonly called heart-leaved moonseed in English, Guduchi or Giloy in Hindi and Gulancha in Bengali belongs to the family Menispermaceae. It is a genetically disparate, large, deciduous climbing shrub with greenish yellow typical flowers, found at higher altitude. 8-10 Recently, the plant is of great interest to researchers across the world due to its medicinal properties like anti-diabetic, anti-inflammatory, anti-oxidant, antiallergic, anti-malarial, anti-periodic, anti-spasmodic, anti-arthritic, antistress, immunomodulatory, hepatoprotective and anti-neoplastic activities. 11 In the present study, an extraction was made for mucilage from the stems of T. cordifolia and investigated for the possibility of use as binder and release modifying material in the formulation of drug in solid dosage forms. Diclofenac sodium, a Non-steroidal Antiinflammatory Drug (NSAID) which is commonly used in the management of pain of various etiologies. 12 Materials and Methods

Indian phytopathology, 2001

The physio- chemical composition of healthy and diseased moringa fruits were investigated at diff... more The physio- chemical composition of healthy and diseased moringa fruits were investigated at different maturity levels. Of varieties examined, Najna contained highest amount of protein, total sugar, reducing sugar, total soluble solid ( TSS), starch and ash while Sajina contained highest amount of total titrable acidity (TTA), moisture, lipid, vitamin C. In both the healthy and diseased conditions, total sugar, reducing sugar, sucrose, TSS, moisture, protein and lipid content were increased but ash, total titrable acidity (TTA) and vitamin C content decreased moderately with the changes of maturity. Starch content changes very rapidly after mature stage. All the nutrient contents under investigation except protein, reducing sugar and ash were decreased after infection of moringa fruits with disease. The activities of all the seven enzymes under investigation were increased after infection of moringa fruits. The activities of catalase and protease increased but that of polyphenol oxidase and ascorbic acid oxidase decreased remarkably with the advancement of maturity stages and the activities of amylase, cellulase and invertase increased upto mature stage and then decreased abruptly.

Pakistan Journal of Biological Sciences, Jan 15, 2001

Indian phytopathology, 1999

Bangladesh Journal of Scientific and Industrial Research, 1970

Two good qualities of mango cultivars namely Fazli and Khirshapat, harvested at the full mature s... more Two good qualities of mango cultivars namely Fazli and Khirshapat, harvested at the full mature stage i.e. begining of ripening, have been stored at-5 o C, 4 o C and 25±2 o C for 10 days and the changes in the content of amylase, invertase, β-galactosidase, starch, total sugar, reducing sugar and non-reducing sugar of the pulp were analyzed after 2-days regular interval of time. The activities of all the enzymes examined in the study were found to be higher in mango-pulp at 25 o C as compared to those in 4 o C or-5 o C. The activities of amylase and invertase decreased after 8-days of storage while that of β-galactosidase increased with the increasing days of storage mango pulp. Total sugar and non-reducing sugar content of mango were increased gradually while starch content was decreased sharply with the increasing days of storage of mature mango. Changes in carbohydrate during storage of mango was found to be associated with the content of amylase, invertase and β-galactosidase. At-5 o C the enzymes as well as carbohydrate content of storage mango pulp changed slightly upto 4days and thereafter the enzyme became fully dormant.

Pakistan Journal of Biological Sciences, Jun 15, 1999

Carbohydrate Research, Aug 1, 1995

Three lectins have been isolated from ant extract of ant egg and purified by gel filtration on Se... more Three lectins have been isolated from ant extract of ant egg and purified by gel filtration on Sephadex G-75 of the 100% ammonium sulfate fraction from the crude extract, followed by ion-exchange chromatography on DEAE-cellulose. All the lectins were homogeneous as judged by polyacrylamide gel electrophoresis. The molecular weights of the lectins, AEL-I, AEL-II, and AEL-III, as estimated by gel filtration, were 195,000, 105,500, and 84,000. All the lectins were found to be composed of four sub-units of unequal sizes, and are glycoproteins with a neutral sugar content of 3.0-7.5%. The lectins agglutinated rat red blood-cells; in a hapten-inhibition study, D-galactose was found to be a better inhibitor for AEL-I and AEL-II whereas D-mannose was a better inhibitor for AEL-III.