Qiaoli Ma - Academia.edu (original) (raw)

Papers by Qiaoli Ma

Research paper thumbnail of First Report of Fusarium oxysporum Causing Fruit Rot on Apricot (Prunus armeniaca) in China

Plant Disease

The author(s) declare no conflict of interest.

Research paper thumbnail of Effects of exogenous 24-epibrassinolide treatment on postharvest quality and resistance of Satsuma mandarin (Citrus unshiu)

Postharvest Biology and Technology, 2015

To elucidate the effects of exogenous brassinosteroids (BRs) treatment on the postharvest behavio... more To elucidate the effects of exogenous brassinosteroids (BRs) treatment on the postharvest behavior of citrus fruit, Satsuma mandarin (Citrus unshiu) fruit were treated with 5 mg L −1 24-epibrassinolide (EBR) and stored at 12-16 • C with a relative humidity of 90-95%. After 50 d treatment, the disease incidence of the control and EBR-treated fruit was 18.7% and 4%, respectively, suggesting that EBR treatment can significantly enhance the postharvest biotic stress tolerance of Satsuma mandarins. Moreover, the fruit quality analysis revealed that EBR treatment induced a rapid weight loss during the first 6 d after treatment, which was similar to the effect of a conditioning treatment, while it exhibited no negative effects on inner quality. Further, we measured the contents of hydrogen peroxide (H 2 O 2 ) and the primary metabolites as well as the expression of the stress-related genes in the fruit pericarp. The results indicated that H 2 O 2 content was significantly increased by EBR treatment. In addition, analyses on the primary metabolites showed that some stress-related metabolites, such as ornithine, proline, GABA, d-xylose and d-galactose, were increased in the EBR-treated fruit. Expression of the stress-related genes was markedly up-regulated in EBR-treated fruit. These results suggest that the effects of EBR on reducing the postharvest disease incidence of citrus fruit may be associated with the accumulation of H 2 O 2 and stress-related metabolites and the induction of stress-related genes.

Research paper thumbnail of Fluroxypyr—a potential surrogate of 2,4-dichlorophenoxyacetic acid for retarding calyx senescence in postharvest citrus fruit

Postharvest Biology and Technology, 2015

Research paper thumbnail of Network Analysis of Postharvest Senescence Process in Citrus Fruits Revealed by Transcriptomic and Metabolomic Profiling

Plant Physiology, 2015

J.C., L.C., J.G.) ORCID ID: 0000-0002-9159-9455 (Y.C.).

Research paper thumbnail of Carotenoid accumulation affects redox status, starch metabolism, and flavonoid/anthocyanin accumulation in citrus

BMC plant biology, Jan 3, 2015

BackgroundCarotenoids are indispensable plant secondary metabolites that are involved in photosyn... more BackgroundCarotenoids are indispensable plant secondary metabolites that are involved in photosynthesis, antioxidation, and phytohormone biosynthesis. Carotenoids are likely involved in other biological functions that have yet to be discovered. In this study, we integrated genomic, biochemical, and cellular studies to gain deep insight into carotenoid-related biological processes in citrus calli overexpressing CrtB (phytoene synthase from Pantoea agglomerans). Fortunella hindsii Swingle (a citrus relative) and Malus hupehensis (a wild apple) calli were also utilized as supporting systems to investigate the effect of altered carotenoid accumulation on carotenoid-related biological processes.ResultsTranscriptomic analysis provided deep insight into the carotenoid-related biological processes of redox status, starch metabolism, and flavonoid/anthocyanin accumulation. By applying biochemical and cytological analyses, we determined that the altered redox status was associated with variat...

Research paper thumbnail of Comprehensive insights on how 2,4-dichlorophenoxyacetic acid retards senescence in post-harvest citrus fruits using transcriptomic and proteomic approaches

Journal of Experimental Botany, 2014

Auxin-like 2,4-dichlorophenoxyacetic acid (2,4-D), a high-efficiency anti-stalling agent for the ... more Auxin-like 2,4-dichlorophenoxyacetic acid (2,4-D), a high-efficiency anti-stalling agent for the post-harvest fresh fruit industry, has had its use restricted due to environmental concerns. However, no other substitutes for 2,4-D are available to the post-harvest industry. Insights into the molecular mechanism underlying the effects of 2,4-D on fruit quality preservation will provide a theoretical basis for exploring new safe and effective anti-stalling agents. This study comprehensively analysed changes in the peel of Olinda Valencia orange [Citrus sinensis (L.) Osbeck] induced by 500 ppm 2,4-D using 'omic'-driven approaches. Transcriptional profiling revealed that transcriptional factor (mainly AP2/ERF, WRKY, and NAC family members), transport, and hormone metabolism genes were over-represented and up-regulated within 24 h post-treatment (HPT). Stress defence genes were up-regulated, while cell wall metabolism genes were down-regulated after 48 HPT. However, secondary metabolism genes, especially phenylpropanoid and lignin biosynthesis-related genes, were over-represented at all the time points. Comparative proteomic analysis indicated that the expression of proteins implicated in stress responses (25%), hormone metabolism, and signal transduction (12%) significantly accumulated at the post-transcriptional level. Hormone levels detected by high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) showed that abscisic acid, salicylic acid, and 2,4-D significantly increased, while ethylene production (detected by gas chromatography) decreased after 2,4-D treatment. In addition, lignin and water content in the fruit peel also increased and the epicuticle wax ultrastructure was modified. In conclusion, 2,4-D retarded fruit senescence by altering the levels of many endogenous hormones and by improving stress defence capabilities by up-regulating defence-related genes and proteins.

Research paper thumbnail of Comparative analysis of surface wax in mature fruits between Satsuma mandarin (Citrus unshiu) and 'Newhall' navel orange (Citrus sinensis) from the perspective of crystal morphology, chemical composition and key gene expression

Food Chemistry, 2014

Surface wax of mature Satsuma mandarin (Citrus unshiu) and 'Newhall' navel orange (Citrus sinensi... more Surface wax of mature Satsuma mandarin (Citrus unshiu) and 'Newhall' navel orange (Citrus sinensis) was analysed by crystal morphology, chemical composition, and gene expression levels. The epicuticular and total waxes of both citrus cultivars were mostly composed of aldehydes, alkanes, fatty acids and primary alcohols. The epicuticular wax accounted for 80% of the total wax in the Newhall fruits and was higher than that in the Satsuma fruits. Scanning electron microscopy showed that larger and more wax platelets were deposited on the surface of Newhall fruits than on the Satsuma fruits. Moreover, the expression levels of genes involved in the wax formation were consistent with the biochemical and crystal morphological analyses. These diversities of fruit wax between the two cultivars may contribute to the differences of fruit postharvest storage properties, which can provide important information for the production of synthetic wax for citrus fruits.

Research paper thumbnail of Integration of Metabolomics and Subcellular Organelle Expression Microarray to Increase Understanding the Organic Acid Changes in Post‐harvest Citrus Fruit

Citric acid plays an important role in fresh fruit flavor and its adaptability to post‐harvest st... more Citric acid plays an important role in fresh fruit flavor and its adaptability to post‐harvest storage conditions.
In order to explore organic acid regulatory mechanisms in post‐harvest citrus fruit, systematic biological
analyses were conducted on stored Hirado Buntan Pummelo (HBP; Citrus grandis) fruits. Highperformance
capillary electrophoresis, subcellular organelle expression microarray, real‐time quantitative
reverse transcription polymerase chain reaction, gas chromatography mass spectrometry (GC‐MS), and
conventional physiological and biochemical analyses were undertaken. The results showed that
the concentration of organic acids in HBP underwent a regular fluctuation. GC‐MS‐based metabolic
profiling indicated that succinic acid, g‐aminobutyric acid (GABA), and glutamine contents increased, but 2‐
oxoglutaric acid content declined, which further confirmed that the GABA shunt may have some regulatory
roles in organic acid catabolismprocesses. In addition, the concentration of organic acids was significantly
correlated with senescence‐related physiological processes, such as hydrogen peroxide content as well as
superoxide dismutase and peroxidase activities, which showed that organic acids could be regarded as
important parameters for measuring citrus fruit post‐harvest senescence processes.

Research paper thumbnail of First Report of Fusarium oxysporum Causing Fruit Rot on Apricot (Prunus armeniaca) in China

Plant Disease

The author(s) declare no conflict of interest.

Research paper thumbnail of Effects of exogenous 24-epibrassinolide treatment on postharvest quality and resistance of Satsuma mandarin (Citrus unshiu)

Postharvest Biology and Technology, 2015

To elucidate the effects of exogenous brassinosteroids (BRs) treatment on the postharvest behavio... more To elucidate the effects of exogenous brassinosteroids (BRs) treatment on the postharvest behavior of citrus fruit, Satsuma mandarin (Citrus unshiu) fruit were treated with 5 mg L −1 24-epibrassinolide (EBR) and stored at 12-16 • C with a relative humidity of 90-95%. After 50 d treatment, the disease incidence of the control and EBR-treated fruit was 18.7% and 4%, respectively, suggesting that EBR treatment can significantly enhance the postharvest biotic stress tolerance of Satsuma mandarins. Moreover, the fruit quality analysis revealed that EBR treatment induced a rapid weight loss during the first 6 d after treatment, which was similar to the effect of a conditioning treatment, while it exhibited no negative effects on inner quality. Further, we measured the contents of hydrogen peroxide (H 2 O 2 ) and the primary metabolites as well as the expression of the stress-related genes in the fruit pericarp. The results indicated that H 2 O 2 content was significantly increased by EBR treatment. In addition, analyses on the primary metabolites showed that some stress-related metabolites, such as ornithine, proline, GABA, d-xylose and d-galactose, were increased in the EBR-treated fruit. Expression of the stress-related genes was markedly up-regulated in EBR-treated fruit. These results suggest that the effects of EBR on reducing the postharvest disease incidence of citrus fruit may be associated with the accumulation of H 2 O 2 and stress-related metabolites and the induction of stress-related genes.

Research paper thumbnail of Fluroxypyr—a potential surrogate of 2,4-dichlorophenoxyacetic acid for retarding calyx senescence in postharvest citrus fruit

Postharvest Biology and Technology, 2015

Research paper thumbnail of Network Analysis of Postharvest Senescence Process in Citrus Fruits Revealed by Transcriptomic and Metabolomic Profiling

Plant Physiology, 2015

J.C., L.C., J.G.) ORCID ID: 0000-0002-9159-9455 (Y.C.).

Research paper thumbnail of Carotenoid accumulation affects redox status, starch metabolism, and flavonoid/anthocyanin accumulation in citrus

BMC plant biology, Jan 3, 2015

BackgroundCarotenoids are indispensable plant secondary metabolites that are involved in photosyn... more BackgroundCarotenoids are indispensable plant secondary metabolites that are involved in photosynthesis, antioxidation, and phytohormone biosynthesis. Carotenoids are likely involved in other biological functions that have yet to be discovered. In this study, we integrated genomic, biochemical, and cellular studies to gain deep insight into carotenoid-related biological processes in citrus calli overexpressing CrtB (phytoene synthase from Pantoea agglomerans). Fortunella hindsii Swingle (a citrus relative) and Malus hupehensis (a wild apple) calli were also utilized as supporting systems to investigate the effect of altered carotenoid accumulation on carotenoid-related biological processes.ResultsTranscriptomic analysis provided deep insight into the carotenoid-related biological processes of redox status, starch metabolism, and flavonoid/anthocyanin accumulation. By applying biochemical and cytological analyses, we determined that the altered redox status was associated with variat...

Research paper thumbnail of Comprehensive insights on how 2,4-dichlorophenoxyacetic acid retards senescence in post-harvest citrus fruits using transcriptomic and proteomic approaches

Journal of Experimental Botany, 2014

Auxin-like 2,4-dichlorophenoxyacetic acid (2,4-D), a high-efficiency anti-stalling agent for the ... more Auxin-like 2,4-dichlorophenoxyacetic acid (2,4-D), a high-efficiency anti-stalling agent for the post-harvest fresh fruit industry, has had its use restricted due to environmental concerns. However, no other substitutes for 2,4-D are available to the post-harvest industry. Insights into the molecular mechanism underlying the effects of 2,4-D on fruit quality preservation will provide a theoretical basis for exploring new safe and effective anti-stalling agents. This study comprehensively analysed changes in the peel of Olinda Valencia orange [Citrus sinensis (L.) Osbeck] induced by 500 ppm 2,4-D using 'omic'-driven approaches. Transcriptional profiling revealed that transcriptional factor (mainly AP2/ERF, WRKY, and NAC family members), transport, and hormone metabolism genes were over-represented and up-regulated within 24 h post-treatment (HPT). Stress defence genes were up-regulated, while cell wall metabolism genes were down-regulated after 48 HPT. However, secondary metabolism genes, especially phenylpropanoid and lignin biosynthesis-related genes, were over-represented at all the time points. Comparative proteomic analysis indicated that the expression of proteins implicated in stress responses (25%), hormone metabolism, and signal transduction (12%) significantly accumulated at the post-transcriptional level. Hormone levels detected by high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) showed that abscisic acid, salicylic acid, and 2,4-D significantly increased, while ethylene production (detected by gas chromatography) decreased after 2,4-D treatment. In addition, lignin and water content in the fruit peel also increased and the epicuticle wax ultrastructure was modified. In conclusion, 2,4-D retarded fruit senescence by altering the levels of many endogenous hormones and by improving stress defence capabilities by up-regulating defence-related genes and proteins.

Research paper thumbnail of Comparative analysis of surface wax in mature fruits between Satsuma mandarin (Citrus unshiu) and 'Newhall' navel orange (Citrus sinensis) from the perspective of crystal morphology, chemical composition and key gene expression

Food Chemistry, 2014

Surface wax of mature Satsuma mandarin (Citrus unshiu) and 'Newhall' navel orange (Citrus sinensi... more Surface wax of mature Satsuma mandarin (Citrus unshiu) and 'Newhall' navel orange (Citrus sinensis) was analysed by crystal morphology, chemical composition, and gene expression levels. The epicuticular and total waxes of both citrus cultivars were mostly composed of aldehydes, alkanes, fatty acids and primary alcohols. The epicuticular wax accounted for 80% of the total wax in the Newhall fruits and was higher than that in the Satsuma fruits. Scanning electron microscopy showed that larger and more wax platelets were deposited on the surface of Newhall fruits than on the Satsuma fruits. Moreover, the expression levels of genes involved in the wax formation were consistent with the biochemical and crystal morphological analyses. These diversities of fruit wax between the two cultivars may contribute to the differences of fruit postharvest storage properties, which can provide important information for the production of synthetic wax for citrus fruits.

Research paper thumbnail of Integration of Metabolomics and Subcellular Organelle Expression Microarray to Increase Understanding the Organic Acid Changes in Post‐harvest Citrus Fruit

Citric acid plays an important role in fresh fruit flavor and its adaptability to post‐harvest st... more Citric acid plays an important role in fresh fruit flavor and its adaptability to post‐harvest storage conditions.
In order to explore organic acid regulatory mechanisms in post‐harvest citrus fruit, systematic biological
analyses were conducted on stored Hirado Buntan Pummelo (HBP; Citrus grandis) fruits. Highperformance
capillary electrophoresis, subcellular organelle expression microarray, real‐time quantitative
reverse transcription polymerase chain reaction, gas chromatography mass spectrometry (GC‐MS), and
conventional physiological and biochemical analyses were undertaken. The results showed that
the concentration of organic acids in HBP underwent a regular fluctuation. GC‐MS‐based metabolic
profiling indicated that succinic acid, g‐aminobutyric acid (GABA), and glutamine contents increased, but 2‐
oxoglutaric acid content declined, which further confirmed that the GABA shunt may have some regulatory
roles in organic acid catabolismprocesses. In addition, the concentration of organic acids was significantly
correlated with senescence‐related physiological processes, such as hydrogen peroxide content as well as
superoxide dismutase and peroxidase activities, which showed that organic acids could be regarded as
important parameters for measuring citrus fruit post‐harvest senescence processes.