Ralf Kühnemuth - Academia.edu (original) (raw)
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ENEA, Italian National Agency for New Technologies, Energy and Sustainable Economic Development
ENEA, Italian National Agency for New Technologies, Energy and Sustainable Economic Development
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Papers by Ralf Kühnemuth
REVIEW OF SCIENTIFIC INSTRUMENTS, 2005
We present an advanced time-correlated single photon counting ͑TCSPC͒ technique that delivers tra... more We present an advanced time-correlated single photon counting ͑TCSPC͒ technique that delivers traditional fluorescence correlation ͑FCS͒ or cross correlation ͑FCCS͒ and fluorescence lifetime data simultaneously. Newly developed electronics allow for detection and registration of single photon events over time periods of hours with picoseconds accuracy. Subsequent software-correlation yields correlation curves covering more than 12 orders of magnitude in time. At the same time, the original data, containing all information accessible by single photon counting techniques, can be analyzed conventionally according to common single molecule fluorescence techniques. Potential applications of the new technique using pulsed or cw laser excitation are discussed.
REVIEW OF SCIENTIFIC INSTRUMENTS, 2005
We present an advanced time-correlated single photon counting ͑TCSPC͒ technique that delivers tra... more We present an advanced time-correlated single photon counting ͑TCSPC͒ technique that delivers traditional fluorescence correlation ͑FCS͒ or cross correlation ͑FCCS͒ and fluorescence lifetime data simultaneously. Newly developed electronics allow for detection and registration of single photon events over time periods of hours with picoseconds accuracy. Subsequent software-correlation yields correlation curves covering more than 12 orders of magnitude in time. At the same time, the original data, containing all information accessible by single photon counting techniques, can be analyzed conventionally according to common single molecule fluorescence techniques. Potential applications of the new technique using pulsed or cw laser excitation are discussed.