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Papers by Chandrahasa Reddy

l o N y Parents and8rothers Wiose support a n d h e lias made it possi611. And to My Teacher, Pro... more l o N y Parents and8rothers Wiose support a n d h e lias made it possi611. And to My Teacher, Pro$ G. Ven&ateswerCu Who prdedinvaCu.a6G? source of strength andinspiration DEPARTMENT OF BIOCHEMISTRY UNIVERSITY COLLEGE OF SCIENCE OSMANIA UNIVERSITY HYDERABAD-500 007, INDIA DECLARATION I here6y declhre that this Ph.D thesis entitiid 3hrsi on &u&s thuringieth ~W R F i n w f d i n the p m d i i ofime&f ~' m f r o m pikmjm: represents the originat research wo* carried out by me in the Department of Biochemistly, Osmania University, Xyderabad under the supervision ofw G. V e n &~& and that I have not submitted this tfiesisforany other degree ofthis orany other University or Institute.

International Journal of Innovative Technology and Exploring Engineering, 2019

Vehicular Ad Hoc Networks (VANET) are useful in implementing a smart transportation system by ena... more Vehicular Ad Hoc Networks (VANET) are useful in implementing a smart transportation system by enabling ad hoc vehicle to vehicle communication. Sybil attack is considered to be one of the most dangerous threats to VANET. Sybil aggressor can produce different phony personalities with false messages to extremely hinder the ordinary elements of wellbeing related applications. In this paper, we are presenting an implementation of a method to detect Sybil attack using received signal strength indicator.

l o N y Parents and8rothers Wiose support a n d h e lias made it possi611. And to My Teacher, Pro... more l o N y Parents and8rothers Wiose support a n d h e lias made it possi611. And to My Teacher, Pro$ G. Ven&ateswerCu Who prdedinvaCu.a6G? source of strength andinspiration DEPARTMENT OF BIOCHEMISTRY UNIVERSITY COLLEGE OF SCIENCE OSMANIA UNIVERSITY HYDERABAD-500 007, INDIA DECLARATION I here6y declhre that this Ph.D thesis entitiid 3hrsi on &u&s thuringieth ~W R F i n w f d i n the p m d i i ofime&f ~' m f r o m pikmjm: represents the originat research wo* carried out by me in the Department of Biochemistly, Osmania University, Xyderabad under the supervision ofw G. V e n &~& and that I have not submitted this tfiesisforany other degree ofthis orany other University or Institute.

Current Microbiology, 2002

The commencement of intracellular protease synthesis was studied by gelatin zymography in Bacillu... more The commencement of intracellular protease synthesis was studied by gelatin zymography in Bacillus thuringiensis ( Btk) HD1, Btk HD73, and a protease-deficient mutant Btk-q derived from the former strain. By gelatin zymography, a 92-kDa protease was detected first at 3 h of sporulation, which continued until 48 h, whereas two other proteases of mol wt 78 and 69 kDa were detectable from 6 h onwards and continued until 48 h of growth in Btk HD1. Similar studies revealed the presence of two major intracellular proteases in Btk HD73 by gelatin zymography, which first appeared at 6 h of sporulation and continued until 48 h of growth. The quantitative azocasein assay confirmed that the total protease activity increases from 3 to 21 h, thereafter reaching a plateau up to 48 h of growth examined, in HD1 and HD73 strains. Btk-q, a protease-deficient mutant, showed traces of protease activity by azocasein analysis that could not be detected by gelatin zymography. The free amino acid pool content was also increased parallel to the way that the protease activity increased in all three strains. However, this increase was found to be low (16-fold) in Btk-q when compared with Btk HD1 and HD73 strains. The following amino acids were detected by paper chromatography in Btk HD1: DL-alanine, L-glutamic acid, L-aspartic acid, tyrosine, tryptophan/methionine/valine, arginine, leucine/norleucine/isoleucine, and glycine, whereas only DL-alanine, L-glutamic acid, and L-aspartic acid were in Btk-q at 24 and 48 h, when the protease activity was maximum.

Biochimie, 2000

The conversion of δ-endoprotoxins of Bacillus thuringiensis to active toxins is mediated by tryps... more The conversion of δ-endoprotoxins of Bacillus thuringiensis to active toxins is mediated by trypsin, insect gut (exogenous) and bacterial (endogenous) proteases. The biochemical aspects of exogenous and endogenous proteases involved in the conversion of protoxin to toxin are reviewed. Perhaps, these proteases also play a role in influencing the host range of toxin and in the development of resistance to toxin.

l o N y Parents and8rothers Wiose support a n d h e lias made it possi611. And to My Teacher, Pro... more l o N y Parents and8rothers Wiose support a n d h e lias made it possi611. And to My Teacher, Pro$ G. Ven&ateswerCu Who prdedinvaCu.a6G? source of strength andinspiration DEPARTMENT OF BIOCHEMISTRY UNIVERSITY COLLEGE OF SCIENCE OSMANIA UNIVERSITY HYDERABAD-500 007, INDIA DECLARATION I here6y declhre that this Ph.D thesis entitiid 3hrsi on &u&s thuringieth ~W R F i n w f d i n the p m d i i ofime&f ~' m f r o m pikmjm: represents the originat research wo* carried out by me in the Department of Biochemistly, Osmania University, Xyderabad under the supervision ofw G. V e n &~& and that I have not submitted this tfiesisforany other degree ofthis orany other University or Institute.

International Journal of Innovative Technology and Exploring Engineering, 2019

Vehicular Ad Hoc Networks (VANET) are useful in implementing a smart transportation system by ena... more Vehicular Ad Hoc Networks (VANET) are useful in implementing a smart transportation system by enabling ad hoc vehicle to vehicle communication. Sybil attack is considered to be one of the most dangerous threats to VANET. Sybil aggressor can produce different phony personalities with false messages to extremely hinder the ordinary elements of wellbeing related applications. In this paper, we are presenting an implementation of a method to detect Sybil attack using received signal strength indicator.

l o N y Parents and8rothers Wiose support a n d h e lias made it possi611. And to My Teacher, Pro... more l o N y Parents and8rothers Wiose support a n d h e lias made it possi611. And to My Teacher, Pro$ G. Ven&ateswerCu Who prdedinvaCu.a6G? source of strength andinspiration DEPARTMENT OF BIOCHEMISTRY UNIVERSITY COLLEGE OF SCIENCE OSMANIA UNIVERSITY HYDERABAD-500 007, INDIA DECLARATION I here6y declhre that this Ph.D thesis entitiid 3hrsi on &u&s thuringieth ~W R F i n w f d i n the p m d i i ofime&f ~' m f r o m pikmjm: represents the originat research wo* carried out by me in the Department of Biochemistly, Osmania University, Xyderabad under the supervision ofw G. V e n &~& and that I have not submitted this tfiesisforany other degree ofthis orany other University or Institute.

Current Microbiology, 2002

The commencement of intracellular protease synthesis was studied by gelatin zymography in Bacillu... more The commencement of intracellular protease synthesis was studied by gelatin zymography in Bacillus thuringiensis ( Btk) HD1, Btk HD73, and a protease-deficient mutant Btk-q derived from the former strain. By gelatin zymography, a 92-kDa protease was detected first at 3 h of sporulation, which continued until 48 h, whereas two other proteases of mol wt 78 and 69 kDa were detectable from 6 h onwards and continued until 48 h of growth in Btk HD1. Similar studies revealed the presence of two major intracellular proteases in Btk HD73 by gelatin zymography, which first appeared at 6 h of sporulation and continued until 48 h of growth. The quantitative azocasein assay confirmed that the total protease activity increases from 3 to 21 h, thereafter reaching a plateau up to 48 h of growth examined, in HD1 and HD73 strains. Btk-q, a protease-deficient mutant, showed traces of protease activity by azocasein analysis that could not be detected by gelatin zymography. The free amino acid pool content was also increased parallel to the way that the protease activity increased in all three strains. However, this increase was found to be low (16-fold) in Btk-q when compared with Btk HD1 and HD73 strains. The following amino acids were detected by paper chromatography in Btk HD1: DL-alanine, L-glutamic acid, L-aspartic acid, tyrosine, tryptophan/methionine/valine, arginine, leucine/norleucine/isoleucine, and glycine, whereas only DL-alanine, L-glutamic acid, and L-aspartic acid were in Btk-q at 24 and 48 h, when the protease activity was maximum.

Biochimie, 2000

The conversion of δ-endoprotoxins of Bacillus thuringiensis to active toxins is mediated by tryps... more The conversion of δ-endoprotoxins of Bacillus thuringiensis to active toxins is mediated by trypsin, insect gut (exogenous) and bacterial (endogenous) proteases. The biochemical aspects of exogenous and endogenous proteases involved in the conversion of protoxin to toxin are reviewed. Perhaps, these proteases also play a role in influencing the host range of toxin and in the development of resistance to toxin.