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Research paper thumbnail of Association Between NR3C1 Gene Polymorphisms and Toxicity Induced by Glucocorticoids Therapy in Saudi Children with Acute Lymphoblastic Leukemia

Asian Pacific Journal of Cancer Prevention : APJCP, 2018

Background: Glucocorticoids (GCs) are key hormones used for the treatment of acute lymphoblastic ... more Background: Glucocorticoids (GCs) are key hormones used for the treatment of acute lymphoblastic leukemia (ALL) in children, but their cytotoxic effects are not well defined. The aim of this study was to evaluate the association between polymorphisms in NR3C1 encoding for protein involved in the GCs metabolism and its role in the development of ALL and the toxicity outcome, in terms of liver toxicity, glucose abnormality and infections, in ALL Saudi children. Methods: The following polymorphisms BCII rs41423247, ER22/23 EK rs6189 and rs6190 and N363S rs6195 in NR3C1 were analyzed in 70 children with ALL treated according to the ALL 2000 study protocol in comparison to 60 control subjects. Treatment toxicities and their association with genotypes were evaluated according to Common Toxicity Criteria (NCI-CTC). Results: This study demonstrated that the NR3C1 did not contribute to the development of childhood ALL. Homozygous ER22/23EK polymorphism was not found in both ALL patients and ...

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Research paper thumbnail of Reticulocyte Hemoglobin-Equivalent Potentially Detects, Diagnoses and Discriminates between Stages of Iron Deficiency with High Sensitivity and Specificity

Journal of Clinical Medicine

Background: Iron deficiency anemia (IDA) is a global health problem affecting the quality of life... more Background: Iron deficiency anemia (IDA) is a global health problem affecting the quality of life of more than 2 billion individuals. The current practice guidelines diagnose and monitor IDA via conventional hematological and iron biomarkers, which take several months before they are corrected under an iron-treatment plan. Reticulocyte hemoglobin equivalent (Ret-He) is used as a marker in most new hematology analyzers to assess iron incorporation into erythrocyte hemoglobin directly. This study aims to examine the efficacy of Ret-He as a marker for iron deficiency (ID) and IDA and investigate whether Ret-He is sensitive to iron therapy. Methods: Two blood samples were drawn from 182 participants for CBC and iron profile measurements. Follow-up samples were drawn from participants with a confirmed diagnosis of ID and/or IDA. Results: Ret-He levels were lower in the ID and IDA groups compared to the control (p < 0.0001), and lower in the IDA group compared to the ID group (p < 0...

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Research paper thumbnail of Protictive Effects of Vitamine e Against Testicular Enzymes Toxicity Induced by Cypermethrin in Mice

International journal of pharma and bio sciences, 2013

Cypermethrin is a synthetic pyrethroid insecticide. It is known for its wide toxic manifestations... more Cypermethrin is a synthetic pyrethroid insecticide. It is known for its wide toxic manifestations. The present work was designed to evaluate the protective role of vitamin E on Cypermethrin intoxication in mice after 14 days. The weight of testis and activity of aspartate aminotransferase (AST), alanine aminotransferase (ALT), acid phosphatase (AcP), alkaline phosphatase (ALP), lactate dehydrogenase (LDH were studied. Male mice were divided into four groups of 10 each: group I served as control and treated with corn oil; group II received cypermethrin (2.8 mg/kg BW) in corn oil. Group III received vitamin E (100 mg/kg BW) ;group IV received both cypermethrin and vitamin E (100 mg/kg BW) the animals were fed by feeding tube through the mouth. The data showed that the exposure of mice to cypermethrin caused a significant decrease in weight of testis and the activities of marker enzymes (AST), (ALT), (AcP) and (ALP) and a significant increase in LDH activity.Co-administration of vitami...

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Research paper thumbnail of Seropositivity and awareness of Toxoplasmosis among University students

Toxoplasma gondii is an obligate intracellular parasite that is infecting about one-third of the ... more Toxoplasma gondii is an obligate intracellular parasite that is infecting about one-third of the world population. Awareness about toxoplasmosis and its transmission can help reduce its prevalence. We examined the degree of awareness about toxoplasmosis among female university students. A total of 127 samples were collected, including 44 samples from health colleges students and 83 samples from other colleges. A questionnaire was used to measure the level of awareness about Toxoplasma gondii. Serum samples were analyzed for the presence of anti-Toxoplasma gondii antibody using ELISA technique. Ninety-four (74%) participants were 18-20 years, 27 (21%) were 21-24 years, and 6 (5%) were above 24 years. Only 11 (9%) students eat raw meat and 21 (17%) students had a tendency to eat undercooked meat. Furthermore, only 6 (5%) students received blood transfusion and 33 (26%) students owned a cat at home. Forty-two (33%) students answered that they know about the disease. The majority of par...

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Research paper thumbnail of Seropositivity and awareness of Toxoplasmosis among University students

Toxoplasma gondii is an obligate intracellular parasite that is infecting about one-third of the ... more Toxoplasma gondii is an obligate intracellular parasite that is infecting about one-third of the world population. Awareness about toxoplasmosis and its transmission can help reduce its prevalence. We examined the degree of awareness about toxoplasmosis among female university students. A total of 127 samples were collected, including 44 samples from health colleges students and 83 samples from other colleges. A questionnaire was used to measure the level of awareness about Toxoplasma gondii. Serum samples were analyzed for the presence of anti-Toxoplasma gondii antibody using ELISA technique. Ninety-four (74%) participants were 18-20 years, 27 (21%) were 21-24 years, and 6 (5%) were above 24 years. Only 11 (9%) students eat raw meat and 21 (17%) students had a tendency to eat undercooked meat. Furthermore, only 6 (5%) students received blood transfusion and 33 (26%) students owned a cat at home. Forty-two (33%) students answered that they know about the disease. The majority of par...

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Research paper thumbnail of DNA damage induced by micellar-delivered doxorubicin and ultrasound: comet assay study

To minimize adverse side effects of chemotherapy, we have developed a micellar drug carrier that ... more To minimize adverse side effects of chemotherapy, we have developed a micellar drug carrier that retains hydrophobic drugs, and then releases the drug by ultrasonic stimulation. This study investigated the DNA damage induced by doxorubicin (DOX) delivered to human leukemia (HL-60) cells from pluronic P-105 micelles with and without the application of ultrasound. The comet assay was used to quantify the amount of DNA damage. No significant DNA damage was observed when the cells were treated with 0.1, 1 and 10 wt% P-105 with or without ultrasound (70 kHz, 1.3 W/cm(2)) for 1 h or for up to 3 h in 10 wt% P-105. However, when cells were incubated with 10 microg/ml free DOX for up to 9 h, DNA damage increased with incubation time (P=0.0011). Exposure of cells to the same concentration of DOX in the presence of 10-wt% P-105 showed no significant DNA damage for up to 9 h of incubation. However, when ultrasound was applied, a rapid and significant increase in DNA damage was observed (P=0.0001). The application of ultrasound causes the release of DOX from micelles or causes the HL-60 cells to take up the micelle encapsulated DOX. Our experiments indicated that the combination of DOX, ultrasound and pluronic P105 causes the largest DNA damage to HL-60 cells. We believe that this technique can be used for controlled drug delivery.

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Research paper thumbnail of The value of transfusion of phenotyped blood units for thalassemia and sickle cell anemia patients at an academic center

Transfusion

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Research paper thumbnail of The influence of polymorphisms in the drug transporter, ABCB1 on the toxicity of glucocorticoids in Saudi children with acute lymphoblastic leukaemia

Pharmacological Reports

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Research paper thumbnail of Alph 1 microglobulin protein as a reliable marker for early diagnosis of renal tubular injury

Twenty five patients with renal tubular damage and ten age-matched healthy control subjects were ... more Twenty five patients with renal tubular damage and ten age-matched healthy control subjects were included in this study. Urinary α1 microglobulin (ά 1M) and ß2 microglobulin (ß2M) were measured and the results were expressed as a ratio to urinary creatinine to avoid variation in urine flow rate. There was a highly significant increase of urinary ά1M/ creatinine ratio and ß2M/ creatinine ratio in patients as compared to healthy control groups. Also, there was a highly significant correlation between ά1M and ß2M (r=0.8, P<0.001). Stability studies revealed that, ά1M was stable in acidic and alkaline urine (P>0.05). On the other hand, ß2M showed marked deterioration in acidic urine (P<0.001). Analysis of receiver operating characteristics (ROC) curve revealed that the diagnostic performance of ά1M was superior to ß2M. The best cut off level of ά1M (13mg/g creatinine) had a diagnostic sensitivity of 95%, specificity 97% and efficacy 96.7%. As regard to ß2M, the best cut off lev...

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Research paper thumbnail of New haplotypes of the ATP synthase subunit 6 gene of mitochondrial DNA are associated with acute lymphoblastic leukemia in Saudi Arabia

Asian Pacific journal of cancer prevention : APJCP, 2014

Acute lymphoblastic leukemia (ALL) is the most common cancer diagnosed in children and represents... more Acute lymphoblastic leukemia (ALL) is the most common cancer diagnosed in children and represents approximately 25% of cancer diagnoses among those younger than 15 years of age. This study investigated substitutions in the ATP synthase subunit 6 gene of mitochondrial DNA (mtDNA) as a potential diagnostic biomarker for early detection and diagnosis of acute lymphoblastic leukemia. Based on mtDNA from 23 subjects diagnosed with acute lymphoblastic leukemia, approximately 465 bp of the ATP synthase subunit 6 gene were amplified and sequenced. The sequencing revealed thirty-one mutations at 14 locations in ATP synthase subunit 6 of mtDNA in the ALL subjects. All were identified as single nucleotide polymorphisms (SNPs) with a homoplasmic pattern. The mutations were distributed between males and females. Novel haplotypes were identified in this investigation: haplotype (G) was recorded in 34% in diagnosed subjects; the second haplotype was (C) with frequency of 13% in ALL subjects. Neith...

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Research paper thumbnail of Novel mutations in the displacement loop of mitochondrial DNA are associated with acute lymphoblastic leukemia: A genetic sequencing study

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Research paper thumbnail of A comparative evaluation of aflatoxin B1 genotoxicity in fish models using the Comet assay

… /Genetic Toxicology and …, 1999

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Research paper thumbnail of Association Between NR3C1 Gene Polymorphisms and Toxicity Induced by Glucocorticoids Therapy in Saudi Children with Acute Lymphoblastic Leukemia

Asian Pacific Journal of Cancer Prevention : APJCP, 2018

Background: Glucocorticoids (GCs) are key hormones used for the treatment of acute lymphoblastic ... more Background: Glucocorticoids (GCs) are key hormones used for the treatment of acute lymphoblastic leukemia (ALL) in children, but their cytotoxic effects are not well defined. The aim of this study was to evaluate the association between polymorphisms in NR3C1 encoding for protein involved in the GCs metabolism and its role in the development of ALL and the toxicity outcome, in terms of liver toxicity, glucose abnormality and infections, in ALL Saudi children. Methods: The following polymorphisms BCII rs41423247, ER22/23 EK rs6189 and rs6190 and N363S rs6195 in NR3C1 were analyzed in 70 children with ALL treated according to the ALL 2000 study protocol in comparison to 60 control subjects. Treatment toxicities and their association with genotypes were evaluated according to Common Toxicity Criteria (NCI-CTC). Results: This study demonstrated that the NR3C1 did not contribute to the development of childhood ALL. Homozygous ER22/23EK polymorphism was not found in both ALL patients and ...

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Research paper thumbnail of Reticulocyte Hemoglobin-Equivalent Potentially Detects, Diagnoses and Discriminates between Stages of Iron Deficiency with High Sensitivity and Specificity

Journal of Clinical Medicine

Background: Iron deficiency anemia (IDA) is a global health problem affecting the quality of life... more Background: Iron deficiency anemia (IDA) is a global health problem affecting the quality of life of more than 2 billion individuals. The current practice guidelines diagnose and monitor IDA via conventional hematological and iron biomarkers, which take several months before they are corrected under an iron-treatment plan. Reticulocyte hemoglobin equivalent (Ret-He) is used as a marker in most new hematology analyzers to assess iron incorporation into erythrocyte hemoglobin directly. This study aims to examine the efficacy of Ret-He as a marker for iron deficiency (ID) and IDA and investigate whether Ret-He is sensitive to iron therapy. Methods: Two blood samples were drawn from 182 participants for CBC and iron profile measurements. Follow-up samples were drawn from participants with a confirmed diagnosis of ID and/or IDA. Results: Ret-He levels were lower in the ID and IDA groups compared to the control (p < 0.0001), and lower in the IDA group compared to the ID group (p < 0...

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Research paper thumbnail of Protictive Effects of Vitamine e Against Testicular Enzymes Toxicity Induced by Cypermethrin in Mice

International journal of pharma and bio sciences, 2013

Cypermethrin is a synthetic pyrethroid insecticide. It is known for its wide toxic manifestations... more Cypermethrin is a synthetic pyrethroid insecticide. It is known for its wide toxic manifestations. The present work was designed to evaluate the protective role of vitamin E on Cypermethrin intoxication in mice after 14 days. The weight of testis and activity of aspartate aminotransferase (AST), alanine aminotransferase (ALT), acid phosphatase (AcP), alkaline phosphatase (ALP), lactate dehydrogenase (LDH were studied. Male mice were divided into four groups of 10 each: group I served as control and treated with corn oil; group II received cypermethrin (2.8 mg/kg BW) in corn oil. Group III received vitamin E (100 mg/kg BW) ;group IV received both cypermethrin and vitamin E (100 mg/kg BW) the animals were fed by feeding tube through the mouth. The data showed that the exposure of mice to cypermethrin caused a significant decrease in weight of testis and the activities of marker enzymes (AST), (ALT), (AcP) and (ALP) and a significant increase in LDH activity.Co-administration of vitami...

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Research paper thumbnail of Seropositivity and awareness of Toxoplasmosis among University students

Toxoplasma gondii is an obligate intracellular parasite that is infecting about one-third of the ... more Toxoplasma gondii is an obligate intracellular parasite that is infecting about one-third of the world population. Awareness about toxoplasmosis and its transmission can help reduce its prevalence. We examined the degree of awareness about toxoplasmosis among female university students. A total of 127 samples were collected, including 44 samples from health colleges students and 83 samples from other colleges. A questionnaire was used to measure the level of awareness about Toxoplasma gondii. Serum samples were analyzed for the presence of anti-Toxoplasma gondii antibody using ELISA technique. Ninety-four (74%) participants were 18-20 years, 27 (21%) were 21-24 years, and 6 (5%) were above 24 years. Only 11 (9%) students eat raw meat and 21 (17%) students had a tendency to eat undercooked meat. Furthermore, only 6 (5%) students received blood transfusion and 33 (26%) students owned a cat at home. Forty-two (33%) students answered that they know about the disease. The majority of par...

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Research paper thumbnail of Seropositivity and awareness of Toxoplasmosis among University students

Toxoplasma gondii is an obligate intracellular parasite that is infecting about one-third of the ... more Toxoplasma gondii is an obligate intracellular parasite that is infecting about one-third of the world population. Awareness about toxoplasmosis and its transmission can help reduce its prevalence. We examined the degree of awareness about toxoplasmosis among female university students. A total of 127 samples were collected, including 44 samples from health colleges students and 83 samples from other colleges. A questionnaire was used to measure the level of awareness about Toxoplasma gondii. Serum samples were analyzed for the presence of anti-Toxoplasma gondii antibody using ELISA technique. Ninety-four (74%) participants were 18-20 years, 27 (21%) were 21-24 years, and 6 (5%) were above 24 years. Only 11 (9%) students eat raw meat and 21 (17%) students had a tendency to eat undercooked meat. Furthermore, only 6 (5%) students received blood transfusion and 33 (26%) students owned a cat at home. Forty-two (33%) students answered that they know about the disease. The majority of par...

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Research paper thumbnail of DNA damage induced by micellar-delivered doxorubicin and ultrasound: comet assay study

To minimize adverse side effects of chemotherapy, we have developed a micellar drug carrier that ... more To minimize adverse side effects of chemotherapy, we have developed a micellar drug carrier that retains hydrophobic drugs, and then releases the drug by ultrasonic stimulation. This study investigated the DNA damage induced by doxorubicin (DOX) delivered to human leukemia (HL-60) cells from pluronic P-105 micelles with and without the application of ultrasound. The comet assay was used to quantify the amount of DNA damage. No significant DNA damage was observed when the cells were treated with 0.1, 1 and 10 wt% P-105 with or without ultrasound (70 kHz, 1.3 W/cm(2)) for 1 h or for up to 3 h in 10 wt% P-105. However, when cells were incubated with 10 microg/ml free DOX for up to 9 h, DNA damage increased with incubation time (P=0.0011). Exposure of cells to the same concentration of DOX in the presence of 10-wt% P-105 showed no significant DNA damage for up to 9 h of incubation. However, when ultrasound was applied, a rapid and significant increase in DNA damage was observed (P=0.0001). The application of ultrasound causes the release of DOX from micelles or causes the HL-60 cells to take up the micelle encapsulated DOX. Our experiments indicated that the combination of DOX, ultrasound and pluronic P105 causes the largest DNA damage to HL-60 cells. We believe that this technique can be used for controlled drug delivery.

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Research paper thumbnail of The value of transfusion of phenotyped blood units for thalassemia and sickle cell anemia patients at an academic center

Transfusion

Bookmarks Related papers MentionsView impact

Research paper thumbnail of The influence of polymorphisms in the drug transporter, ABCB1 on the toxicity of glucocorticoids in Saudi children with acute lymphoblastic leukaemia

Pharmacological Reports

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Alph 1 microglobulin protein as a reliable marker for early diagnosis of renal tubular injury

Twenty five patients with renal tubular damage and ten age-matched healthy control subjects were ... more Twenty five patients with renal tubular damage and ten age-matched healthy control subjects were included in this study. Urinary α1 microglobulin (ά 1M) and ß2 microglobulin (ß2M) were measured and the results were expressed as a ratio to urinary creatinine to avoid variation in urine flow rate. There was a highly significant increase of urinary ά1M/ creatinine ratio and ß2M/ creatinine ratio in patients as compared to healthy control groups. Also, there was a highly significant correlation between ά1M and ß2M (r=0.8, P<0.001). Stability studies revealed that, ά1M was stable in acidic and alkaline urine (P>0.05). On the other hand, ß2M showed marked deterioration in acidic urine (P<0.001). Analysis of receiver operating characteristics (ROC) curve revealed that the diagnostic performance of ά1M was superior to ß2M. The best cut off level of ά1M (13mg/g creatinine) had a diagnostic sensitivity of 95%, specificity 97% and efficacy 96.7%. As regard to ß2M, the best cut off lev...

Bookmarks Related papers MentionsView impact

Research paper thumbnail of New haplotypes of the ATP synthase subunit 6 gene of mitochondrial DNA are associated with acute lymphoblastic leukemia in Saudi Arabia

Asian Pacific journal of cancer prevention : APJCP, 2014

Acute lymphoblastic leukemia (ALL) is the most common cancer diagnosed in children and represents... more Acute lymphoblastic leukemia (ALL) is the most common cancer diagnosed in children and represents approximately 25% of cancer diagnoses among those younger than 15 years of age. This study investigated substitutions in the ATP synthase subunit 6 gene of mitochondrial DNA (mtDNA) as a potential diagnostic biomarker for early detection and diagnosis of acute lymphoblastic leukemia. Based on mtDNA from 23 subjects diagnosed with acute lymphoblastic leukemia, approximately 465 bp of the ATP synthase subunit 6 gene were amplified and sequenced. The sequencing revealed thirty-one mutations at 14 locations in ATP synthase subunit 6 of mtDNA in the ALL subjects. All were identified as single nucleotide polymorphisms (SNPs) with a homoplasmic pattern. The mutations were distributed between males and females. Novel haplotypes were identified in this investigation: haplotype (G) was recorded in 34% in diagnosed subjects; the second haplotype was (C) with frequency of 13% in ALL subjects. Neith...

Bookmarks Related papers MentionsView impact

Research paper thumbnail of Novel mutations in the displacement loop of mitochondrial DNA are associated with acute lymphoblastic leukemia: A genetic sequencing study

Bookmarks Related papers MentionsView impact

Research paper thumbnail of A comparative evaluation of aflatoxin B1 genotoxicity in fish models using the Comet assay

… /Genetic Toxicology and …, 1999

Bookmarks Related papers MentionsView impact