Ritesh Shukla - Academia.edu (original) (raw)

Papers by Ritesh Shukla

Research paper thumbnail of Additional file 1: of Development of submerged and successive latent fingerprints: a comparative study

Development of submerged & successive prints on various substrates using Robin powder blue (RPB) ... more Development of submerged & successive prints on various substrates using Robin powder blue (RPB) and silver magnetic dual powder (SMDP). (DOCX 1440 kb)

Research paper thumbnail of Evaluation of diallelic STR markers with inter-population allelic database for their usefulness in paternity trios in the Central Indian population

Annals of Human Biology, 2021

Abstract Background Most of the forensic DNA laboratories have migrated to new generation STR kit... more Abstract Background Most of the forensic DNA laboratories have migrated to new generation STR kits of 6 dye chemistry with more than 20 autosomal STRs. The population-specific databases of such STR markers are lacking in many regions. Aim To evaluate the effect of the inter-population database in 100 paternity trios with no inconsistencies at 23 STRs. Subjects and methods 100 paternity trios were evaluated considering inter-population allelic frequency values for calculation of Combined Paternity Index (CPI) and Probability of Paternity (POP). Results No significant variation (p < 0.05) among the allele frequencies at the interpopulation level was observed. The number of obligate alleles and the likelihood of transferring obligate alleles from the putative father showed a positive correlation (p < 0.005) with Power of Discrimination (PD), Polymorphic Information Content (PIC), Power of Exclusion (PE), Paternity Index (PI), Observed and Expected Heterozygosity (Ho and He), and a statistically significant negative correlation (p < 0.005) with Matching Probability (Pm). The average Combined Paternity Index (CPI) and Probability of Paternity (POP) did not show any statistically significant difference (p < 0.05) at the interpopulation level. Conclusion The allelic database showed no effect on the CPI and POP in 100 paternity trios. This suggests no urgent need for using population-specific databases for statistical evaluation of paternity trios without inconsistencies.

Research paper thumbnail of Genotoxic Potential of Nanoparticles: Structural and Functional Modifications in DNA

Frontiers in Genetics, 2021

The rapid advancement of nanotechnology enhances the production of different nanoparticles that m... more The rapid advancement of nanotechnology enhances the production of different nanoparticles that meet the demand of various fields like biomedical sciences, industrial, material sciences and biotechnology, etc. This technological development increases the chances of nanoparticles exposure to human beings, which can threaten their health. It is well known that various cellular processes (transcription, translation, and replication during cell proliferation, cell cycle, cell differentiation) in which genetic materials (DNA and RNA) are involved play a vital role to maintain any structural and functional modification into it. When nanoparticles come into the vicinity of the cellular system, chances of uptake become high due to their small size. This cellular uptake of nanoparticles enhances its interaction with DNA, leading to structural and functional modification (DNA damage/repair, DNA methylation) into the DNA. These modifications exhibit adverse effects on the cellular system, cons...

Research paper thumbnail of Estimation of real-infection and immunity against SARS-CoV-2 in Indian populations

Infection born by Coronavirus SARS-CoV-2 has swept the world within a time of a few months. It ha... more Infection born by Coronavirus SARS-CoV-2 has swept the world within a time of a few months. It has created a devastating effect on humanity with social and economic depressions. Europe and America were the hardest hit continents. India has also lost several lives, making the country fourth most deadly worldwide. However, the infection and death rate per million and the case fatality ratio in India were substantially lower than many of the developed nations. Several factors have been proposed including the genetics. One of the important facts is that a large chunk of Indian population is asymptomatic to the SARS-CoV-2 infection. Thus, the real infection in India is much higher than the reported number of cases. Therefore, the majority of people are already immune in the country. To understand the dynamics of real infection as well as level of immunity against SARS-CoV-2, we have performed antibody testing (serosurveillance) in the urban region of fourteen Indian districts encompassin...

Research paper thumbnail of Forensic analytical approaches to the dating of documents: An overview

Microchemical Journal, 2021

Abstract Document dating is one of the most challenging areas in the field of questioned document... more Abstract Document dating is one of the most challenging areas in the field of questioned document examination. Determination of age of document becomes of prime importance in the cases involving the document having historical value, or the document in question bearing a date, that is disputed. The dating of documents can be done by focusing on different aspects of a document, i.e., dating through paper, through inks, through a sequence of intersecting lines, through handwriting, etc. This article furnishes a broad overview of the dating documents techniques and instruments based on the various methods and their possible applications and limitations. The document dating may be performed using either or a combination of static, dynamic and supplementary approaches. The static approach includes time tags, chemical composition, elemental profiling. The dynamic approach includes ink, paper and toner ageing. The supplementary methods comprise accelerated ageing techniques, chemometric approaches, constructing the chronology of documents, analyzing luminescent components, radiocarbon dating and nanotechnology-based approaches. This article brings to fore the utilization of the different facets of the document for dating purposes. It presents the summarized form of the different methods and techniques, including some less focused ones.

Research paper thumbnail of New perspective of nanotechnology: role in preventive forensic

Egyptian Journal of Forensic Sciences, 2018

Background: Generally, material scientist discussing new-fangled nanotechnology research and its ... more Background: Generally, material scientist discussing new-fangled nanotechnology research and its conversation turns to the commercial aspects or potential issues around health, safety, and the environment. In recent years, few of them have been shown interest to apply nanotechnology for forensic purposes which are related to societal or community issues. Main text: In forensic science, nanotechnology majorly is applied to develop reactive ('smart') materials; microchip technology; nanomanipulators; nanoimaging tools for visualization. These advances in forensic technologies have mainly been focused on analytical techniques. These techniques are based on nanomaterials that are able to provide either improved performance over conventional techniques or enable to collect information from a crime scene that would not otherwise have been possible ever. Moreover, some crucial forensic issues such as cost and time effectiveness, wide availability, accuracy, and reliability of results are major concerns in forensic investigation and security. Conclusion: With the help of this review article, an attempt was made to elucidate how nanotechnologies could be crucial in addressing current forensic investigation issues such as collection and analysis of evidence from the crime scene and how these technologies may be helpful in preventive forensic and security measures.

Research paper thumbnail of Development of submerged and successive latent fingerprints: a comparative study

Egyptian Journal of Forensic Sciences, 2019

Background: The use of water to destroy evidences in criminal cases is common. It is uncommon to ... more Background: The use of water to destroy evidences in criminal cases is common. It is uncommon to believe the usefulness of evidences recovered underwater in terms of its forensic significance regarding personal identification especially by the investigating officers, who are responsible to collect and analyse the evidences. In this study, two main factors were considered which may impact the condition of fingerprint evidences: firstly, the time duration for which the evidence remains submerged in water (0.5 h, 24 h, 48 h, 120 h), and secondly, the succession or the number of prints given by the same finger one after the other (5 subsequent prints). Results: The result of this study revealed the successful development of latent fingerprint using Robin blue and silver magnetic powders on 8 different non-porous surfaces. Conclusion: The developed prints provide significant individual characteristics; hence, the evidentiary value of the objects found submerged in water should not be undervalued.

Research paper thumbnail of Multifunctional Silver-Cellulose Nanocomposite as a Promising Plasmonic Sensing Platform

Journal of nanoscience and nanotechnology, 2018

Cellulose paper based multifunctional nanoplatform shows great promise towards molecular sensing ... more Cellulose paper based multifunctional nanoplatform shows great promise towards molecular sensing and diagnostics application due to their sustainability, biocompatibility, affordability, environmental appeal and broad chemical modification capabilities. Herein, we have effectively synthesized plasmonic silver-cellulose nanocomposite (SCN) using complete green and in situ approach using the hydroxyl groups of cellulose paper acting as the reducing agent and stabilizing agent. The fabricated SCN were investigated and characterized by SEM, UV-Vis, DLS, PXRD and EDX. The important influencing parameters such as temperature and pH were optimized during the fabrication of SCN. Besides, this SCN functionalized with 3-aminopropyltriethoxysilane and has been exploited for monitoring of fluoride ion from water samples with distinguishing F- from a wide range of environmental prevalent ions. These remarkable properties of SCN have been used as rapid, portable test panel which could be simple, ...

Research paper thumbnail of Synthesis of biocompatible iron oxide nanoparticles as a drug delivery vehicle

International journal of nanomedicine, 2018

Over the last decade, there has been growing interest in developing novel nanoparticles (NPs) for... more Over the last decade, there has been growing interest in developing novel nanoparticles (NPs) for biomedical applications. A safe-by-design approach was used in this study to synthesize biocompatible iron oxide NPs. The size of the particles obtained was ~100 nm. Although these NPs were significantly (<0.05) internalized in MCF-7 (human breast adenocarcinoma cell line) cells, no adverse effect was observed in the cells as assessed by cytotoxicity assays (neutral red uptake and 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) and cell cycle analysis. Our data demonstrate the potential of iron oxide NPs as a biocompatible carrier for targeted drug delivery.

Research paper thumbnail of Cellular internalization and antioxidant activity of cerium oxide nanoparticles in human monocytic leukemia cells

International journal of nanomedicine, 2018

Overproduction of free radicals contributes to oxidative stress and inflammation leading to vario... more Overproduction of free radicals contributes to oxidative stress and inflammation leading to various disease conditions. Cerium oxide nanoparticles (nanoceria) have been shown to scavenge free radicals and have the potential for being used as a therapeutic agent in disease conditions. Therefore, in the present study, human monocytic leukemia cells (THP-1) were used as a model to evaluate the uptake and free radical scavenging activity of nanoceria. Our data showed a significant (<0.05) increase in the internalization of nanoceria in a concentration-dependent (10-100 µg/mL) manner in THP-1 cells. Although no cytotoxicity was observed at these concentrations, nanoceria significantly (<0.05) reduced the amount of reactive oxygen species. This was evident by a significant (<0.05) decrease in the 2,7-dichlorofluorescein diacetate fluorescence observed in flow cytometry and fluorescence microscopy. The present study shows that nanoceria have therapeutic potential in diseases such ...

Research paper thumbnail of 2.45 GHz low level CW microwave radiation affects embryo implantation sites and single strand DNA damage in brain cells of mice, mus musculus

2009 International Conference on Emerging Trends in Electronic and Photonic Devices & Systems, 2009

Vineet Prakash Singh, Priyanka Singh and Chandra Mohini Chaturvedi* Department of Zoology, Banara... more Vineet Prakash Singh, Priyanka Singh and Chandra Mohini Chaturvedi* Department of Zoology, Banaras Hindu University Varanasi -221005, INDIA E-mail: cmcbhu@yahoo.com* ... Ritesh K. Shukla, Alok Dhawan Indian Institute of Toxicology Research Lucknow -226 ...

Research paper thumbnail of 2.45 GHz (CW) MICROWAVE IRRADIATION ALTERS CIRCADIAN ORGANIZATION, SPATIAL MEMORY, DNA STRUCTURE IN THE BRAIN CELLS AND BLOOD CELL COUNTS OF MALE MICE, MUS MUSCULUS

Progress In Electromagnetics Research B, 2011

Present study examines biological effects of 2.45 GHz microwave radiation in Parkes strain mice. ... more Present study examines biological effects of 2.45 GHz microwave radiation in Parkes strain mice. Forty-day-old mice were exposed to CW (continuous wave) microwave radiation (2 h/day for 30 days). Locomotor activity was recorded on running wheel for 12 days prior to microwave exposure (pre-exposure), 7 days during the first week of exposure (short-term exposure) and another 7-day spell during the last week of the 30-day exposure period (long-term exposure). Morris water maze test was performed from 17th to 22nd day of exposure. At the termination of the exposure, blood was processed for hematological parameters, brain for comet assay, epididymis for sperm count and motility and serum for SGOT (serum glutamate oxaloacetate transaminase) and SGPT (serum glutamate

Research paper thumbnail of Surface functionalization of quantum dots for biological applications

Advances in Colloid and Interface Science, 2015

Quantum dots are a group of inorganic nanomaterials exhibiting exceptional optical and electronic... more Quantum dots are a group of inorganic nanomaterials exhibiting exceptional optical and electronic properties which impart distinct advantages over traditional fluorescent organic dyes in terms of tunable broad excitation and narrow emission spectra, signal brightness, high quantum yield and photo-stability. Aqueous solubility and surface functionalization are the most common problems for QDs employed in biological research. This review addresses the recent research progress made to improve aqueous solubility, functionalization of biomolecules to QD surface and the poorly understood chemistry involved in the steps of bio-functionalization of such nanoparticles.

Research paper thumbnail of ROS-mediated genotoxicity induced by titanium dioxide nanoparticles in human epidermal cells

Toxicology in Vitro, 2011

Titanium dioxide nanoparticles (TiO 2 NPs) are among the top five NPs used in consumer products, ... more Titanium dioxide nanoparticles (TiO 2 NPs) are among the top five NPs used in consumer products, paints and pharmaceutical preparations. Since, exposure to such nanoparticles is mainly through the skin and inhalation, the present study was conducted in the human epidermal cells (A431). A mild cytotoxic response of TiO 2 NPs was observed as evident by the MTT and NR uptake assays after 48 h of exposure. However, a statistically significant (p < 0.05) induction in the DNA damage was observed by the Fpgmodified Comet assay in cells exposed to 0.8 lg/ml TiO 2 NPs (2.20 ± 0.26 vs. control 1.24 ± 0.04) and higher concentrations for 6 h. A significant (p < 0.05) induction in micronucleus formation was also observed at the above concentration (14.67 ± 1.20 vs. control 9.33 ± 1.00). TiO 2 NPs elicited a significant (p < 0.05) reduction in glutathione (15.76%) with a concomitant increase in lipid hydroperoxide (60.51%; p < 0.05) and reactive oxygen species (ROS) generation (49.2%; p < 0.05) after 6 h exposure. Our data demonstrate that TiO 2 NPs have a mild cytotoxic potential. However, they induce ROS and oxidative stress leading to oxidative DNA damage and micronucleus formation, a probable mechanism of genotoxicity. This is perhaps the first study on human skin cells demonstrating the cytotoxic and genotoxic potential of TiO 2 NPs.

Research paper thumbnail of Cytotoxicity assessment of ZnO nanoparticles on human epidermal cells

Molecular Cytogenetics, 2014

Research paper thumbnail of TiO2 nanoparticles induce cytotoxicity and genotoxicity in human alveolar cells

Molecular Cytogenetics, 2014

Research paper thumbnail of TiO2 nanoparticles induced micronucleus formation in human liver (HepG2) cells: comparison of conventional and flow cytometry based methods

Molecular Cytogenetics, 2014

Vallabani et al.: TiO2 nanoparticles induced micronucleus formation in human liver (HepG2) cells:... more Vallabani et al.: TiO2 nanoparticles induced micronucleus formation in human liver (HepG2) cells: comparison of conventional and flow cytometry based methods. Molecular Cytogenetics 2014 7(Suppl 1):P79.

Research paper thumbnail of PEGylated nanoceria protect human epidermal cells from reactive oxygen species

Molecular Cytogenetics, 2014

Research paper thumbnail of TiO2 NPs induced hepatic injury in mammals: a mechanistic approach

Molecular Cytogenetics, 2014

Research paper thumbnail of BSA coated gold nanoparticles exhibit size dependent interaction with lung cancer (A549) cells

Molecular Cytogenetics, 2014

Research paper thumbnail of Additional file 1: of Development of submerged and successive latent fingerprints: a comparative study

Development of submerged & successive prints on various substrates using Robin powder blue (RPB) ... more Development of submerged & successive prints on various substrates using Robin powder blue (RPB) and silver magnetic dual powder (SMDP). (DOCX 1440 kb)

Research paper thumbnail of Evaluation of diallelic STR markers with inter-population allelic database for their usefulness in paternity trios in the Central Indian population

Annals of Human Biology, 2021

Abstract Background Most of the forensic DNA laboratories have migrated to new generation STR kit... more Abstract Background Most of the forensic DNA laboratories have migrated to new generation STR kits of 6 dye chemistry with more than 20 autosomal STRs. The population-specific databases of such STR markers are lacking in many regions. Aim To evaluate the effect of the inter-population database in 100 paternity trios with no inconsistencies at 23 STRs. Subjects and methods 100 paternity trios were evaluated considering inter-population allelic frequency values for calculation of Combined Paternity Index (CPI) and Probability of Paternity (POP). Results No significant variation (p < 0.05) among the allele frequencies at the interpopulation level was observed. The number of obligate alleles and the likelihood of transferring obligate alleles from the putative father showed a positive correlation (p < 0.005) with Power of Discrimination (PD), Polymorphic Information Content (PIC), Power of Exclusion (PE), Paternity Index (PI), Observed and Expected Heterozygosity (Ho and He), and a statistically significant negative correlation (p < 0.005) with Matching Probability (Pm). The average Combined Paternity Index (CPI) and Probability of Paternity (POP) did not show any statistically significant difference (p < 0.05) at the interpopulation level. Conclusion The allelic database showed no effect on the CPI and POP in 100 paternity trios. This suggests no urgent need for using population-specific databases for statistical evaluation of paternity trios without inconsistencies.

Research paper thumbnail of Genotoxic Potential of Nanoparticles: Structural and Functional Modifications in DNA

Frontiers in Genetics, 2021

The rapid advancement of nanotechnology enhances the production of different nanoparticles that m... more The rapid advancement of nanotechnology enhances the production of different nanoparticles that meet the demand of various fields like biomedical sciences, industrial, material sciences and biotechnology, etc. This technological development increases the chances of nanoparticles exposure to human beings, which can threaten their health. It is well known that various cellular processes (transcription, translation, and replication during cell proliferation, cell cycle, cell differentiation) in which genetic materials (DNA and RNA) are involved play a vital role to maintain any structural and functional modification into it. When nanoparticles come into the vicinity of the cellular system, chances of uptake become high due to their small size. This cellular uptake of nanoparticles enhances its interaction with DNA, leading to structural and functional modification (DNA damage/repair, DNA methylation) into the DNA. These modifications exhibit adverse effects on the cellular system, cons...

Research paper thumbnail of Estimation of real-infection and immunity against SARS-CoV-2 in Indian populations

Infection born by Coronavirus SARS-CoV-2 has swept the world within a time of a few months. It ha... more Infection born by Coronavirus SARS-CoV-2 has swept the world within a time of a few months. It has created a devastating effect on humanity with social and economic depressions. Europe and America were the hardest hit continents. India has also lost several lives, making the country fourth most deadly worldwide. However, the infection and death rate per million and the case fatality ratio in India were substantially lower than many of the developed nations. Several factors have been proposed including the genetics. One of the important facts is that a large chunk of Indian population is asymptomatic to the SARS-CoV-2 infection. Thus, the real infection in India is much higher than the reported number of cases. Therefore, the majority of people are already immune in the country. To understand the dynamics of real infection as well as level of immunity against SARS-CoV-2, we have performed antibody testing (serosurveillance) in the urban region of fourteen Indian districts encompassin...

Research paper thumbnail of Forensic analytical approaches to the dating of documents: An overview

Microchemical Journal, 2021

Abstract Document dating is one of the most challenging areas in the field of questioned document... more Abstract Document dating is one of the most challenging areas in the field of questioned document examination. Determination of age of document becomes of prime importance in the cases involving the document having historical value, or the document in question bearing a date, that is disputed. The dating of documents can be done by focusing on different aspects of a document, i.e., dating through paper, through inks, through a sequence of intersecting lines, through handwriting, etc. This article furnishes a broad overview of the dating documents techniques and instruments based on the various methods and their possible applications and limitations. The document dating may be performed using either or a combination of static, dynamic and supplementary approaches. The static approach includes time tags, chemical composition, elemental profiling. The dynamic approach includes ink, paper and toner ageing. The supplementary methods comprise accelerated ageing techniques, chemometric approaches, constructing the chronology of documents, analyzing luminescent components, radiocarbon dating and nanotechnology-based approaches. This article brings to fore the utilization of the different facets of the document for dating purposes. It presents the summarized form of the different methods and techniques, including some less focused ones.

Research paper thumbnail of New perspective of nanotechnology: role in preventive forensic

Egyptian Journal of Forensic Sciences, 2018

Background: Generally, material scientist discussing new-fangled nanotechnology research and its ... more Background: Generally, material scientist discussing new-fangled nanotechnology research and its conversation turns to the commercial aspects or potential issues around health, safety, and the environment. In recent years, few of them have been shown interest to apply nanotechnology for forensic purposes which are related to societal or community issues. Main text: In forensic science, nanotechnology majorly is applied to develop reactive ('smart') materials; microchip technology; nanomanipulators; nanoimaging tools for visualization. These advances in forensic technologies have mainly been focused on analytical techniques. These techniques are based on nanomaterials that are able to provide either improved performance over conventional techniques or enable to collect information from a crime scene that would not otherwise have been possible ever. Moreover, some crucial forensic issues such as cost and time effectiveness, wide availability, accuracy, and reliability of results are major concerns in forensic investigation and security. Conclusion: With the help of this review article, an attempt was made to elucidate how nanotechnologies could be crucial in addressing current forensic investigation issues such as collection and analysis of evidence from the crime scene and how these technologies may be helpful in preventive forensic and security measures.

Research paper thumbnail of Development of submerged and successive latent fingerprints: a comparative study

Egyptian Journal of Forensic Sciences, 2019

Background: The use of water to destroy evidences in criminal cases is common. It is uncommon to ... more Background: The use of water to destroy evidences in criminal cases is common. It is uncommon to believe the usefulness of evidences recovered underwater in terms of its forensic significance regarding personal identification especially by the investigating officers, who are responsible to collect and analyse the evidences. In this study, two main factors were considered which may impact the condition of fingerprint evidences: firstly, the time duration for which the evidence remains submerged in water (0.5 h, 24 h, 48 h, 120 h), and secondly, the succession or the number of prints given by the same finger one after the other (5 subsequent prints). Results: The result of this study revealed the successful development of latent fingerprint using Robin blue and silver magnetic powders on 8 different non-porous surfaces. Conclusion: The developed prints provide significant individual characteristics; hence, the evidentiary value of the objects found submerged in water should not be undervalued.

Research paper thumbnail of Multifunctional Silver-Cellulose Nanocomposite as a Promising Plasmonic Sensing Platform

Journal of nanoscience and nanotechnology, 2018

Cellulose paper based multifunctional nanoplatform shows great promise towards molecular sensing ... more Cellulose paper based multifunctional nanoplatform shows great promise towards molecular sensing and diagnostics application due to their sustainability, biocompatibility, affordability, environmental appeal and broad chemical modification capabilities. Herein, we have effectively synthesized plasmonic silver-cellulose nanocomposite (SCN) using complete green and in situ approach using the hydroxyl groups of cellulose paper acting as the reducing agent and stabilizing agent. The fabricated SCN were investigated and characterized by SEM, UV-Vis, DLS, PXRD and EDX. The important influencing parameters such as temperature and pH were optimized during the fabrication of SCN. Besides, this SCN functionalized with 3-aminopropyltriethoxysilane and has been exploited for monitoring of fluoride ion from water samples with distinguishing F- from a wide range of environmental prevalent ions. These remarkable properties of SCN have been used as rapid, portable test panel which could be simple, ...

Research paper thumbnail of Synthesis of biocompatible iron oxide nanoparticles as a drug delivery vehicle

International journal of nanomedicine, 2018

Over the last decade, there has been growing interest in developing novel nanoparticles (NPs) for... more Over the last decade, there has been growing interest in developing novel nanoparticles (NPs) for biomedical applications. A safe-by-design approach was used in this study to synthesize biocompatible iron oxide NPs. The size of the particles obtained was ~100 nm. Although these NPs were significantly (<0.05) internalized in MCF-7 (human breast adenocarcinoma cell line) cells, no adverse effect was observed in the cells as assessed by cytotoxicity assays (neutral red uptake and 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) and cell cycle analysis. Our data demonstrate the potential of iron oxide NPs as a biocompatible carrier for targeted drug delivery.

Research paper thumbnail of Cellular internalization and antioxidant activity of cerium oxide nanoparticles in human monocytic leukemia cells

International journal of nanomedicine, 2018

Overproduction of free radicals contributes to oxidative stress and inflammation leading to vario... more Overproduction of free radicals contributes to oxidative stress and inflammation leading to various disease conditions. Cerium oxide nanoparticles (nanoceria) have been shown to scavenge free radicals and have the potential for being used as a therapeutic agent in disease conditions. Therefore, in the present study, human monocytic leukemia cells (THP-1) were used as a model to evaluate the uptake and free radical scavenging activity of nanoceria. Our data showed a significant (<0.05) increase in the internalization of nanoceria in a concentration-dependent (10-100 µg/mL) manner in THP-1 cells. Although no cytotoxicity was observed at these concentrations, nanoceria significantly (<0.05) reduced the amount of reactive oxygen species. This was evident by a significant (<0.05) decrease in the 2,7-dichlorofluorescein diacetate fluorescence observed in flow cytometry and fluorescence microscopy. The present study shows that nanoceria have therapeutic potential in diseases such ...

Research paper thumbnail of 2.45 GHz low level CW microwave radiation affects embryo implantation sites and single strand DNA damage in brain cells of mice, mus musculus

2009 International Conference on Emerging Trends in Electronic and Photonic Devices & Systems, 2009

Vineet Prakash Singh, Priyanka Singh and Chandra Mohini Chaturvedi* Department of Zoology, Banara... more Vineet Prakash Singh, Priyanka Singh and Chandra Mohini Chaturvedi* Department of Zoology, Banaras Hindu University Varanasi -221005, INDIA E-mail: cmcbhu@yahoo.com* ... Ritesh K. Shukla, Alok Dhawan Indian Institute of Toxicology Research Lucknow -226 ...

Research paper thumbnail of 2.45 GHz (CW) MICROWAVE IRRADIATION ALTERS CIRCADIAN ORGANIZATION, SPATIAL MEMORY, DNA STRUCTURE IN THE BRAIN CELLS AND BLOOD CELL COUNTS OF MALE MICE, MUS MUSCULUS

Progress In Electromagnetics Research B, 2011

Present study examines biological effects of 2.45 GHz microwave radiation in Parkes strain mice. ... more Present study examines biological effects of 2.45 GHz microwave radiation in Parkes strain mice. Forty-day-old mice were exposed to CW (continuous wave) microwave radiation (2 h/day for 30 days). Locomotor activity was recorded on running wheel for 12 days prior to microwave exposure (pre-exposure), 7 days during the first week of exposure (short-term exposure) and another 7-day spell during the last week of the 30-day exposure period (long-term exposure). Morris water maze test was performed from 17th to 22nd day of exposure. At the termination of the exposure, blood was processed for hematological parameters, brain for comet assay, epididymis for sperm count and motility and serum for SGOT (serum glutamate oxaloacetate transaminase) and SGPT (serum glutamate

Research paper thumbnail of Surface functionalization of quantum dots for biological applications

Advances in Colloid and Interface Science, 2015

Quantum dots are a group of inorganic nanomaterials exhibiting exceptional optical and electronic... more Quantum dots are a group of inorganic nanomaterials exhibiting exceptional optical and electronic properties which impart distinct advantages over traditional fluorescent organic dyes in terms of tunable broad excitation and narrow emission spectra, signal brightness, high quantum yield and photo-stability. Aqueous solubility and surface functionalization are the most common problems for QDs employed in biological research. This review addresses the recent research progress made to improve aqueous solubility, functionalization of biomolecules to QD surface and the poorly understood chemistry involved in the steps of bio-functionalization of such nanoparticles.

Research paper thumbnail of ROS-mediated genotoxicity induced by titanium dioxide nanoparticles in human epidermal cells

Toxicology in Vitro, 2011

Titanium dioxide nanoparticles (TiO 2 NPs) are among the top five NPs used in consumer products, ... more Titanium dioxide nanoparticles (TiO 2 NPs) are among the top five NPs used in consumer products, paints and pharmaceutical preparations. Since, exposure to such nanoparticles is mainly through the skin and inhalation, the present study was conducted in the human epidermal cells (A431). A mild cytotoxic response of TiO 2 NPs was observed as evident by the MTT and NR uptake assays after 48 h of exposure. However, a statistically significant (p < 0.05) induction in the DNA damage was observed by the Fpgmodified Comet assay in cells exposed to 0.8 lg/ml TiO 2 NPs (2.20 ± 0.26 vs. control 1.24 ± 0.04) and higher concentrations for 6 h. A significant (p < 0.05) induction in micronucleus formation was also observed at the above concentration (14.67 ± 1.20 vs. control 9.33 ± 1.00). TiO 2 NPs elicited a significant (p < 0.05) reduction in glutathione (15.76%) with a concomitant increase in lipid hydroperoxide (60.51%; p < 0.05) and reactive oxygen species (ROS) generation (49.2%; p < 0.05) after 6 h exposure. Our data demonstrate that TiO 2 NPs have a mild cytotoxic potential. However, they induce ROS and oxidative stress leading to oxidative DNA damage and micronucleus formation, a probable mechanism of genotoxicity. This is perhaps the first study on human skin cells demonstrating the cytotoxic and genotoxic potential of TiO 2 NPs.

Research paper thumbnail of Cytotoxicity assessment of ZnO nanoparticles on human epidermal cells

Molecular Cytogenetics, 2014

Research paper thumbnail of TiO2 nanoparticles induce cytotoxicity and genotoxicity in human alveolar cells

Molecular Cytogenetics, 2014

Research paper thumbnail of TiO2 nanoparticles induced micronucleus formation in human liver (HepG2) cells: comparison of conventional and flow cytometry based methods

Molecular Cytogenetics, 2014

Vallabani et al.: TiO2 nanoparticles induced micronucleus formation in human liver (HepG2) cells:... more Vallabani et al.: TiO2 nanoparticles induced micronucleus formation in human liver (HepG2) cells: comparison of conventional and flow cytometry based methods. Molecular Cytogenetics 2014 7(Suppl 1):P79.

Research paper thumbnail of PEGylated nanoceria protect human epidermal cells from reactive oxygen species

Molecular Cytogenetics, 2014

Research paper thumbnail of TiO2 NPs induced hepatic injury in mammals: a mechanistic approach

Molecular Cytogenetics, 2014

Research paper thumbnail of BSA coated gold nanoparticles exhibit size dependent interaction with lung cancer (A549) cells

Molecular Cytogenetics, 2014