Ronald Virag - Academia.edu (original) (raw)
Papers by Ronald Virag
Journal Des Maladies Vasculaires, 2002
Objectif de l'etude : L'oxyde nitrique (NO) est considere comme le neurotransmetteur esse... more Objectif de l'etude : L'oxyde nitrique (NO) est considere comme le neurotransmetteur essentiel au declenchement et au maintien de l'erection. Il est responsable de la dilatation arterielle observee apres occlusion de la circulation peripherique. Le but de ce travail a ete d'etudier la possibilite d'evaluer la reserve en NO des corps caverneux en mesurant les variations du calibre des arteres caverneuses apres occlusion de la circulation penienne. Patients et methodes : Quatre-vingt quinze patients, 22 ayant des erections normales (groupe temoin) et 73 avec divers degres de dysfonctionnement erectile (DE) evalues par l'indice international de la fonction erectile (IIEF) et l'indice d'activite erectile (IAE) ont ete testes puis separes, a la suite de l'etude pluridisciplinaire de leur etiologie en deux sous-groupes : organique (n = 47) et psychologique (n = 26). Pour chaque patient le diametre d'une des deux arteres caverneuses a ete mesure par ...
Sexual Health Issues, 2017
Background: Platelet-Rich Plasma (PRP) has been successfully used for different medical condition... more Background: Platelet-Rich Plasma (PRP) has been successfully used for different medical conditions, to help regenerate fibrotic or inflammatory tissues. Peyronie's disease (PD) could be a target for such treatment. In this study, PRP in combination with hyaluronic acid (HA) is proposed as an innovative treatment for this condition. Objectives: To assess the effects of repeated intralesional injections inside the tunica albuginea (TA) with a combination of PRP and HA in PD patients. Design, setting, and participants: 90 patients, naive of any prior interventional treatment, were injected with 8 ml of PRP combined with HA directly in the diseased area of TA under ultrasound (US) guidance. Injections were repeated 4 times within 2 months. Additional monthly sessions were done, if necessary. Outcome measurements and statistical analysis: Penile deformation, TA thickening, presence and size of calcifications, and questionnaires evaluating both PD and sexual function. Global results were classified into satisfactory improvement (I+) unsatisfactory (I-) and unchanged (U). Results and limitations: Angulation and thickening were both significantly improved after 4 sessions and continuously improved with the number of sessions, to achieve a mean final improvement of 39.65 +/-24.83% (p<0.001) (CI 14.26;18.82) and 26.23+/-10.29% (p<0.001) (CI 0.99;1.22), respectively. Younger patients achieved better results. 73.3% of the patients belonged to I+ group; 67.8% felt an improvement, 62.2% a decrease of their deformation, 46.7% had easier sexual intercourses and 70% judged the treatment as positive. Limitations included the absence of a control group. Conclusions: Repeated intralesional injections of PRP-HA improve significantly penile deformation and thickening of the TA. This fully natural treatment may stand as a first line treatment of PD.
Journal of cellular biochemistry, Jan 30, 2016
Erectile dysfunction (ED) is a complication of diabetes, condition responsible for causing endoth... more Erectile dysfunction (ED) is a complication of diabetes, condition responsible for causing endothelial dysfunction (EDys) and hampering repair mechanisms. However, scarce information is available linking vasculogenesis mediated by Endothelial Progenitor Cells (EPCs) and diabetes-associated ED. Furthermore, it remains to be elucidated if glycemic control plays a role on EPCs functions, EPCs modulators and penile vascular health. We evaluated the effects of diabetes and insulin therapy on bone marrow (BM) and circulating EPCs, testosterone and systemic/penile Stromal Derived Factor-1 alpha (SDF-1α) expression. Male Wistar rats were divided into groups: age-matched controls, 8-weeks streptozotocin-induced type 1 diabetics and insulin-treated 8-weeks diabetics. EPCs were identified by flow cytometry for CD34/CD133/VEGFR2/CXCR4 antigens. Systemic SDF-1α and testosterone levels were evaluated by ELISA. Penile SDF-1α protein expression was assessed, in experimental and human diabetic caver...
European Urology Supplements, 2011
follow-up of 8 months, 2 out of 15 patients have a dosable PSA. The former (case #2) had a Gleaso... more follow-up of 8 months, 2 out of 15 patients have a dosable PSA. The former (case #2) had a Gleason 3+3 pT2a prostate cancer, and the second PSA is stable. We hypothesized a benign prostate gland remnant, and decided to watchful wait. The latter (case #13) had a final pathology report of Gleason 4+3 pT2c, with positive surgical margin. He is scheduled for pelvic lymphadenectomy and radiotherapy. Conclusions: We demonstrated not only the feasibility, but also the possible advantages concerning functional results of a new intrafascial anatomical approach for RALP, passing through the Douglas space. Even though the oncological results are preliminary (and overlapping with classical RALP), our functional results at a very early follow-up resemble those obtained several months after classical RALP. The anatomical rationale for having better results is strong, but well-designed, controlled, and possibly randomized studies are needed to evaluate the superiority of this technique.
Journal of Sexual Medicine
European Endocrinology, 2009
Erectile dysfunction (ED) is a common complication of diabetes, with a prevalence ranging from 15... more Erectile dysfunction (ED) is a common complication of diabetes, with a prevalence ranging from 15 to 55%. The basis underlying diabetesassociated ED is multifactorial, involving changes in peripheral nerve activity and alterations in endothelial cell function. Due to the complexity of this pathology, the development of experimental models has been crucial in evaluating and translating fundamental results into clinical diabetes-associated ED. The concept of hard-to-treat patients, such as men with diabetes, is now fully accepted due to the complex mechanisms involved. In these men, the response to common oral treatments with phosphodiesterase type 5 inhibitors (PDE5Is) is far from desired, and maximal doses of the drugs are often needed. In addition, diabetes is commonly associated with other co-morbidities, such as hypertension, hypercholesterolaemia and obesity, clusters of the metabolic syndrome (MetS). ED is considered an early warning sentinel for coronary artery disease, just a...
La Revue de Médecine Interne, 1997
Journal des maladies vasculaires, 2002
Nitric oxide (NO) is now considered as the main contributor to elicit and maintain erection. It i... more Nitric oxide (NO) is now considered as the main contributor to elicit and maintain erection. It is also established that flow-dependent dilatation of conduit arteries is mediated by NO. The present study was designed to verify the possibility of assessing the penile NO content when measuring the post occlusive vasodilatation of the cavernous arteries. Ninety five subjects, 22 with normal erections (control group) and 73 with various degrees of erectile dysfunction (ED), measured with the international index of erectile function (IIEF) and erectile activity index (IAE). Multidisciplinary evaluation helped to separate two subgroups: mainly organic (n=47) and mainly psychologic (n=26). The diameter of one of the two cavernous arteries was measured by duplex-scan before and after a five-minute complete occlusion of penile vascular flow. The criterion retained was the maximum percentage of dilatation achieved within 45 to 90 sec after the release of the occlusion. Means were compared in ...
Chirurgie; mémoires de l'Académie de chirurgie, 1997
From 1985 to 1995, 172 patients (149 on self intracavernous injection of vasoactive drugs, 16 wit... more From 1985 to 1995, 172 patients (149 on self intracavernous injection of vasoactive drugs, 16 with Sickle cell disease, 6 surgical patients under heparin therapy, and 1 after oral administration of trazodone), having experienced one or several episodes of priapism, lasting from 3 h to 8 days have been treated or submitted to self medication with alpha-agonist agents (eprephrine, phenylephrine or etilefrine) with an eventual drainage of the corporae. All episodes have disappeared and sexual function was preserved. A conservative treatment of priapism has been designed using corporal drainage and intracavernous etilefrine for acute priapism; as well as preventive treatment for those of the patients exposed to Sickle cell disease to avoid surgery and its frequent fribrotic sequelae, leading to impotence in 50% of the cases.
Sexologies, 2008
Conclusions: Preliminary results supported that the cognitive and emotional factors, such as the ... more Conclusions: Preliminary results supported that the cognitive and emotional factors, such as the schemas and affect, and the general psychopathology are related with the sexual functioning in general women. Women with a poorer sexual functioning present a significant lack of positive affect, a stronger activation of different cognitive schemas and higher levels of psychopathology.
European Urology Supplements, 2008
European Urology Supplements, Volume 7, Issue 3, Pages 294, March 2008, Authors:C. Costa; R. Soar... more European Urology Supplements, Volume 7, Issue 3, Pages 294, March 2008, Authors:C. Costa; R. Soares; A. Castela; V. Hastert; P. Vendeira; R. Virag.
European Urology Supplements, 2012
Progrès en Urologie, 2010
Dysfonction érectile et cellules endothéliales caverneuses 189 prise en charge clinique de la DE.... more Dysfonction érectile et cellules endothéliales caverneuses 189 prise en charge clinique de la DE. Les différentes explorations fonctionnelles qui en découlent et le mode d'action des traitements actuels seront abordés dans une prochaine synthèse.
Urology, 1996
Objectives. Priapism is a common and currently unsatisfactorily managed complication of sickle ce... more Objectives. Priapism is a common and currently unsatisfactorily managed complication of sickle cell disease (SCD). In June 1994, 6 SCD patients received a new therapeutic regimen to prevent the occurrence and recurrence of priapism. Methods. The patients (S with SS and 1 with SC) were adults and had frequent episodes of stuttering priapism (SP), and two of them had had acute episodes (AP) lasting more than 3 hours. The treatment consists of preventive oral administration of the alpha-adrenergic agent etilefrine, and self-administered intracavernous injection (SlCl) of the same agent to reverse episodes lasting more than 1 hour. Results. Since the beginning of treatment, all patients were protected against AP, 4 patients had no recurrence with the oral treatment alone, 2 had to use SICI, 1 occasionally and 1 constantly. There was no modification of sexual activity and no complications. Blood pressure was unaffected. Conclusions. This treatment is simple, cheap, and self-administered. It should be proposed to all patients with SCD in all geographic areas as part of an educational program for active prevention of this severe complication. UROLOGY® 47: 777-781, 1996. EVALUATION Table I reports the general status of SCD and the previous complications other than priapism for patients. The history
Molecular Genetics and Metabolism, 2012
Diabetes-induced metabolic derangements promote endothelial malfunction, contributing to erectile... more Diabetes-induced metabolic derangements promote endothelial malfunction, contributing to erectile dysfunction (ED). However, it remains unclear which angiogenic molecular mechanisms are deregulated in diabetic corpus cavernosum (CC). We investigated early and late alterations in cavernosal angiogenic gene expression associated to diabetes. Angiogenic changes were assessed in penile tissue of streptozotocin-induced Wistar rats, in an early (2-week) and established stage (8-week) of diabetes. Differentially expressed genes were identified by microarrays and expression data validated by quantitative real-time PCR (qrt-PCR). At protein level, quantitative immunohistochemistry confirmed the arrays data and dual immunofluorescence for selected alterations and α-smooth muscle actin (α-SMA) identified the cellular location of target proteins. The selected differentially expressed genes were also evaluated in human non-diabetic and diabetic CC by quantitative immunolabeling. At 2-week diabetes there was no differential gene expression between non-diabetic and diabetic CC. At 8-week, 10 genes were found down-regulated in diabetics. The results were validated by qrt-PCR for the insulin-like growth factor-1 (Igf1) and the natriuretic peptide receptor-1 (Npr1) genes. Dual immunofluorescence for IGF-1/ α-SMA showed predominant localization of IGF-1 in SM. NPR-1 expression was diffuse and mostly present in trabecular fibroblasts and SM. Quantitative immunostaining confirmed the decreased expression of both proteins in diabetic tissues. Concordantly, we detected a significant reduction in IGF-1 and NPR-1 protein expressions in human diabetic samples. Microarray analysis identified 10 angiogenic-related molecules deregulated in CC of established diabetes. Among them, IGF-1 and NPR-1 were significantly down-regulated and might result in preventive/therapeutic targets for ED management.
The Lancet, 1985
Mean plasma diazepam levels for the two formulations. 704 (53) ng/ml for valium. Neither of these... more Mean plasma diazepam levels for the two formulations. 704 (53) ng/ml for valium. Neither of these differences was significant. Nor was there any significant difference in the area under the concentration/time curve at 10, 15, or 60 min. We cannot confirm the findings of Fee et al that plasma diazepam concentrations after injection of diazemuls are significantly lower than those after valium.
The Journal of Urology, 2011
VEGFexpressing control. Additionally, the total number of implanted cells expressing human nuclea... more VEGFexpressing control. Additionally, the total number of implanted cells expressing human nuclear markers was significantly higher in VEGF-expressing grafts. More cells also expressed endothelial markers (CD 31 and vWF) and smooth muscle markers (␣-smooth muscle actin, desmin and myosin) in the VEGF group. Finally, the number of peripheral nerve fibers observed in the USC/Ad-VEGF plus endothelial cells group increased compared to the other controls in vivo. However, a few human nuclei staining were observed in the newly-formed nerve fibers, suggesting that most of the regenerated nerve fibers were derived from host nerve tissue. CONCLUSIONS: VEGF over-expression in implanted USC enhanced the in vivo survival of these cells and increased endothelial and smooth muscle differentiation of USC. Neovascularization and nerve regeneration significantly increased within the USC/Ad-VEGF grafts. This approach might have important clinical implications for urological cell therapy, including the correction of neurovascular ED.
The Journal of Urology, 2011
regulating the smooth muscle tone. In this study, important scientific evidence in the developmen... more regulating the smooth muscle tone. In this study, important scientific evidence in the development of new drug for erectile dysfunction using natural products has been established.
Journal Des Maladies Vasculaires, 2002
Objectif de l'etude : L'oxyde nitrique (NO) est considere comme le neurotransmetteur esse... more Objectif de l'etude : L'oxyde nitrique (NO) est considere comme le neurotransmetteur essentiel au declenchement et au maintien de l'erection. Il est responsable de la dilatation arterielle observee apres occlusion de la circulation peripherique. Le but de ce travail a ete d'etudier la possibilite d'evaluer la reserve en NO des corps caverneux en mesurant les variations du calibre des arteres caverneuses apres occlusion de la circulation penienne. Patients et methodes : Quatre-vingt quinze patients, 22 ayant des erections normales (groupe temoin) et 73 avec divers degres de dysfonctionnement erectile (DE) evalues par l'indice international de la fonction erectile (IIEF) et l'indice d'activite erectile (IAE) ont ete testes puis separes, a la suite de l'etude pluridisciplinaire de leur etiologie en deux sous-groupes : organique (n = 47) et psychologique (n = 26). Pour chaque patient le diametre d'une des deux arteres caverneuses a ete mesure par ...
Sexual Health Issues, 2017
Background: Platelet-Rich Plasma (PRP) has been successfully used for different medical condition... more Background: Platelet-Rich Plasma (PRP) has been successfully used for different medical conditions, to help regenerate fibrotic or inflammatory tissues. Peyronie's disease (PD) could be a target for such treatment. In this study, PRP in combination with hyaluronic acid (HA) is proposed as an innovative treatment for this condition. Objectives: To assess the effects of repeated intralesional injections inside the tunica albuginea (TA) with a combination of PRP and HA in PD patients. Design, setting, and participants: 90 patients, naive of any prior interventional treatment, were injected with 8 ml of PRP combined with HA directly in the diseased area of TA under ultrasound (US) guidance. Injections were repeated 4 times within 2 months. Additional monthly sessions were done, if necessary. Outcome measurements and statistical analysis: Penile deformation, TA thickening, presence and size of calcifications, and questionnaires evaluating both PD and sexual function. Global results were classified into satisfactory improvement (I+) unsatisfactory (I-) and unchanged (U). Results and limitations: Angulation and thickening were both significantly improved after 4 sessions and continuously improved with the number of sessions, to achieve a mean final improvement of 39.65 +/-24.83% (p<0.001) (CI 14.26;18.82) and 26.23+/-10.29% (p<0.001) (CI 0.99;1.22), respectively. Younger patients achieved better results. 73.3% of the patients belonged to I+ group; 67.8% felt an improvement, 62.2% a decrease of their deformation, 46.7% had easier sexual intercourses and 70% judged the treatment as positive. Limitations included the absence of a control group. Conclusions: Repeated intralesional injections of PRP-HA improve significantly penile deformation and thickening of the TA. This fully natural treatment may stand as a first line treatment of PD.
Journal of cellular biochemistry, Jan 30, 2016
Erectile dysfunction (ED) is a complication of diabetes, condition responsible for causing endoth... more Erectile dysfunction (ED) is a complication of diabetes, condition responsible for causing endothelial dysfunction (EDys) and hampering repair mechanisms. However, scarce information is available linking vasculogenesis mediated by Endothelial Progenitor Cells (EPCs) and diabetes-associated ED. Furthermore, it remains to be elucidated if glycemic control plays a role on EPCs functions, EPCs modulators and penile vascular health. We evaluated the effects of diabetes and insulin therapy on bone marrow (BM) and circulating EPCs, testosterone and systemic/penile Stromal Derived Factor-1 alpha (SDF-1α) expression. Male Wistar rats were divided into groups: age-matched controls, 8-weeks streptozotocin-induced type 1 diabetics and insulin-treated 8-weeks diabetics. EPCs were identified by flow cytometry for CD34/CD133/VEGFR2/CXCR4 antigens. Systemic SDF-1α and testosterone levels were evaluated by ELISA. Penile SDF-1α protein expression was assessed, in experimental and human diabetic caver...
European Urology Supplements, 2011
follow-up of 8 months, 2 out of 15 patients have a dosable PSA. The former (case #2) had a Gleaso... more follow-up of 8 months, 2 out of 15 patients have a dosable PSA. The former (case #2) had a Gleason 3+3 pT2a prostate cancer, and the second PSA is stable. We hypothesized a benign prostate gland remnant, and decided to watchful wait. The latter (case #13) had a final pathology report of Gleason 4+3 pT2c, with positive surgical margin. He is scheduled for pelvic lymphadenectomy and radiotherapy. Conclusions: We demonstrated not only the feasibility, but also the possible advantages concerning functional results of a new intrafascial anatomical approach for RALP, passing through the Douglas space. Even though the oncological results are preliminary (and overlapping with classical RALP), our functional results at a very early follow-up resemble those obtained several months after classical RALP. The anatomical rationale for having better results is strong, but well-designed, controlled, and possibly randomized studies are needed to evaluate the superiority of this technique.
Journal of Sexual Medicine
European Endocrinology, 2009
Erectile dysfunction (ED) is a common complication of diabetes, with a prevalence ranging from 15... more Erectile dysfunction (ED) is a common complication of diabetes, with a prevalence ranging from 15 to 55%. The basis underlying diabetesassociated ED is multifactorial, involving changes in peripheral nerve activity and alterations in endothelial cell function. Due to the complexity of this pathology, the development of experimental models has been crucial in evaluating and translating fundamental results into clinical diabetes-associated ED. The concept of hard-to-treat patients, such as men with diabetes, is now fully accepted due to the complex mechanisms involved. In these men, the response to common oral treatments with phosphodiesterase type 5 inhibitors (PDE5Is) is far from desired, and maximal doses of the drugs are often needed. In addition, diabetes is commonly associated with other co-morbidities, such as hypertension, hypercholesterolaemia and obesity, clusters of the metabolic syndrome (MetS). ED is considered an early warning sentinel for coronary artery disease, just a...
La Revue de Médecine Interne, 1997
Journal des maladies vasculaires, 2002
Nitric oxide (NO) is now considered as the main contributor to elicit and maintain erection. It i... more Nitric oxide (NO) is now considered as the main contributor to elicit and maintain erection. It is also established that flow-dependent dilatation of conduit arteries is mediated by NO. The present study was designed to verify the possibility of assessing the penile NO content when measuring the post occlusive vasodilatation of the cavernous arteries. Ninety five subjects, 22 with normal erections (control group) and 73 with various degrees of erectile dysfunction (ED), measured with the international index of erectile function (IIEF) and erectile activity index (IAE). Multidisciplinary evaluation helped to separate two subgroups: mainly organic (n=47) and mainly psychologic (n=26). The diameter of one of the two cavernous arteries was measured by duplex-scan before and after a five-minute complete occlusion of penile vascular flow. The criterion retained was the maximum percentage of dilatation achieved within 45 to 90 sec after the release of the occlusion. Means were compared in ...
Chirurgie; mémoires de l'Académie de chirurgie, 1997
From 1985 to 1995, 172 patients (149 on self intracavernous injection of vasoactive drugs, 16 wit... more From 1985 to 1995, 172 patients (149 on self intracavernous injection of vasoactive drugs, 16 with Sickle cell disease, 6 surgical patients under heparin therapy, and 1 after oral administration of trazodone), having experienced one or several episodes of priapism, lasting from 3 h to 8 days have been treated or submitted to self medication with alpha-agonist agents (eprephrine, phenylephrine or etilefrine) with an eventual drainage of the corporae. All episodes have disappeared and sexual function was preserved. A conservative treatment of priapism has been designed using corporal drainage and intracavernous etilefrine for acute priapism; as well as preventive treatment for those of the patients exposed to Sickle cell disease to avoid surgery and its frequent fribrotic sequelae, leading to impotence in 50% of the cases.
Sexologies, 2008
Conclusions: Preliminary results supported that the cognitive and emotional factors, such as the ... more Conclusions: Preliminary results supported that the cognitive and emotional factors, such as the schemas and affect, and the general psychopathology are related with the sexual functioning in general women. Women with a poorer sexual functioning present a significant lack of positive affect, a stronger activation of different cognitive schemas and higher levels of psychopathology.
European Urology Supplements, 2008
European Urology Supplements, Volume 7, Issue 3, Pages 294, March 2008, Authors:C. Costa; R. Soar... more European Urology Supplements, Volume 7, Issue 3, Pages 294, March 2008, Authors:C. Costa; R. Soares; A. Castela; V. Hastert; P. Vendeira; R. Virag.
European Urology Supplements, 2012
Progrès en Urologie, 2010
Dysfonction érectile et cellules endothéliales caverneuses 189 prise en charge clinique de la DE.... more Dysfonction érectile et cellules endothéliales caverneuses 189 prise en charge clinique de la DE. Les différentes explorations fonctionnelles qui en découlent et le mode d'action des traitements actuels seront abordés dans une prochaine synthèse.
Urology, 1996
Objectives. Priapism is a common and currently unsatisfactorily managed complication of sickle ce... more Objectives. Priapism is a common and currently unsatisfactorily managed complication of sickle cell disease (SCD). In June 1994, 6 SCD patients received a new therapeutic regimen to prevent the occurrence and recurrence of priapism. Methods. The patients (S with SS and 1 with SC) were adults and had frequent episodes of stuttering priapism (SP), and two of them had had acute episodes (AP) lasting more than 3 hours. The treatment consists of preventive oral administration of the alpha-adrenergic agent etilefrine, and self-administered intracavernous injection (SlCl) of the same agent to reverse episodes lasting more than 1 hour. Results. Since the beginning of treatment, all patients were protected against AP, 4 patients had no recurrence with the oral treatment alone, 2 had to use SICI, 1 occasionally and 1 constantly. There was no modification of sexual activity and no complications. Blood pressure was unaffected. Conclusions. This treatment is simple, cheap, and self-administered. It should be proposed to all patients with SCD in all geographic areas as part of an educational program for active prevention of this severe complication. UROLOGY® 47: 777-781, 1996. EVALUATION Table I reports the general status of SCD and the previous complications other than priapism for patients. The history
Molecular Genetics and Metabolism, 2012
Diabetes-induced metabolic derangements promote endothelial malfunction, contributing to erectile... more Diabetes-induced metabolic derangements promote endothelial malfunction, contributing to erectile dysfunction (ED). However, it remains unclear which angiogenic molecular mechanisms are deregulated in diabetic corpus cavernosum (CC). We investigated early and late alterations in cavernosal angiogenic gene expression associated to diabetes. Angiogenic changes were assessed in penile tissue of streptozotocin-induced Wistar rats, in an early (2-week) and established stage (8-week) of diabetes. Differentially expressed genes were identified by microarrays and expression data validated by quantitative real-time PCR (qrt-PCR). At protein level, quantitative immunohistochemistry confirmed the arrays data and dual immunofluorescence for selected alterations and α-smooth muscle actin (α-SMA) identified the cellular location of target proteins. The selected differentially expressed genes were also evaluated in human non-diabetic and diabetic CC by quantitative immunolabeling. At 2-week diabetes there was no differential gene expression between non-diabetic and diabetic CC. At 8-week, 10 genes were found down-regulated in diabetics. The results were validated by qrt-PCR for the insulin-like growth factor-1 (Igf1) and the natriuretic peptide receptor-1 (Npr1) genes. Dual immunofluorescence for IGF-1/ α-SMA showed predominant localization of IGF-1 in SM. NPR-1 expression was diffuse and mostly present in trabecular fibroblasts and SM. Quantitative immunostaining confirmed the decreased expression of both proteins in diabetic tissues. Concordantly, we detected a significant reduction in IGF-1 and NPR-1 protein expressions in human diabetic samples. Microarray analysis identified 10 angiogenic-related molecules deregulated in CC of established diabetes. Among them, IGF-1 and NPR-1 were significantly down-regulated and might result in preventive/therapeutic targets for ED management.
The Lancet, 1985
Mean plasma diazepam levels for the two formulations. 704 (53) ng/ml for valium. Neither of these... more Mean plasma diazepam levels for the two formulations. 704 (53) ng/ml for valium. Neither of these differences was significant. Nor was there any significant difference in the area under the concentration/time curve at 10, 15, or 60 min. We cannot confirm the findings of Fee et al that plasma diazepam concentrations after injection of diazemuls are significantly lower than those after valium.
The Journal of Urology, 2011
VEGFexpressing control. Additionally, the total number of implanted cells expressing human nuclea... more VEGFexpressing control. Additionally, the total number of implanted cells expressing human nuclear markers was significantly higher in VEGF-expressing grafts. More cells also expressed endothelial markers (CD 31 and vWF) and smooth muscle markers (␣-smooth muscle actin, desmin and myosin) in the VEGF group. Finally, the number of peripheral nerve fibers observed in the USC/Ad-VEGF plus endothelial cells group increased compared to the other controls in vivo. However, a few human nuclei staining were observed in the newly-formed nerve fibers, suggesting that most of the regenerated nerve fibers were derived from host nerve tissue. CONCLUSIONS: VEGF over-expression in implanted USC enhanced the in vivo survival of these cells and increased endothelial and smooth muscle differentiation of USC. Neovascularization and nerve regeneration significantly increased within the USC/Ad-VEGF grafts. This approach might have important clinical implications for urological cell therapy, including the correction of neurovascular ED.
The Journal of Urology, 2011
regulating the smooth muscle tone. In this study, important scientific evidence in the developmen... more regulating the smooth muscle tone. In this study, important scientific evidence in the development of new drug for erectile dysfunction using natural products has been established.