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Research paper thumbnail of イネ根圏界面におけるひ素耐性細菌株のバイオレメディエーション可能性の評価【Powered by NICT】

Journal of Environmental Sciences-china, 2016

Research paper thumbnail of Marf-mediated mitochondrial fusion is imperative for the development and functioning of indirect flight muscles (IFMs) in drosophila

Experimental Cell Research, 2021

Dynamic changes in mitochondrial shape and size are vital for mitochondrial health and for tissue... more Dynamic changes in mitochondrial shape and size are vital for mitochondrial health and for tissue development and function. Adult Drosophila indirect flight muscles contain densely packed mitochondria. We show here that mitochondrial fusion is critical during early muscle development (in pupa) and that silencing of the outer mitochondrial membrane fusion gene, Marf, in muscles results in smaller mitochondria that are functionally defective. This leads to abnormal muscle development resulting in muscle dysfunction in adult flies. However, post-developmental silencing of Marf has no obvious effects on mitochondrial and muscle phenotype in adult flies, indicating the importance of mitochondrial fusion during early muscle development.

Research paper thumbnail of Evolving paradigms on the interplay of mitochondrial Hsp70 chaperone system in cell survival and senescence

Critical Reviews in Biochemistry and Molecular Biology, 2019

The role of mitochondria within a cell has grown beyond being the prime source of cellular energy... more The role of mitochondria within a cell has grown beyond being the prime source of cellular energy to one of the major signaling platforms. Recent evidence provides several insights into the crucial roles of mitochondrial chaperones in regulating the organellar response to external triggers. The mitochondrial Hsp70 (mtHsp70/Mortalin/Grp75) chaperone system plays a critical role in the maintenance of proteostasis balance in the organelle. Defects in mtHsp70 network result in attenuated protein transport and misfolding of polypeptides leading to mitochondrial dysfunction. The functions of Hsp70 are primarily governed by J-protein cochaperones. Although human mitochondria possess a single Hsp70, its multifunctionality is characterized by the presence of multiple specific J-proteins. Several studies have shown a potential association of Hsp70 and J-proteins with diverse pathological states that are not limited to their canonical role as chaperones. The role of mitochondrial Hsp70 and its co-chaperones in disease pathogenesis has not been critically reviewed in recent years. We evaluated some of the cellular interfaces where Hsp70 machinery associated with pathophysiological conditions, particularly in context of tumorigenesis and neurodegeneration. The mitochondrial Hsp70 machinery shows a variable localization and integrates multiple components of the cellular processes with varied phenotypic consequences. Although Hsp70 and J-proteins function synergistically in proteins folding, their precise involvement in pathological conditions is mainly idiosyncratic. This machinery is associated with a heterogeneous set of molecules during the progression of a disorder. However, the precise binding to the substrate for a specific physiological response under a disease subtype is still an undocumented area of analysis.

Research paper thumbnail of A Redox Modulatory Mn3O4Nanozyme with Multi-Enzyme Activity Provides Efficient Cytoprotection to Human Cells in a Parkinson's Disease Model

Angewandte Chemie, 2017

Angabe der unten stehenden Digitalobjekt-Identifizierungsnummer (DOI) zitiert werden. Die deutsch... more Angabe der unten stehenden Digitalobjekt-Identifizierungsnummer (DOI) zitiert werden. Die deutsche Übersetzung wird gemeinsam mit der endgültigen englischen Fassung erscheinen. Die endgültige englische Fassung (Version of Record) wird ehestmöglich nach dem Redigieren und einem Korrekturgang als Early-View-Beitrag erscheinen und kann sich naturgemäß von der AA-Fassung unterscheiden. Leser sollten daher die endgültige Fassung, sobald sie veröffentlicht ist, verwenden. Für die AA-Fassung trägt der Autor die alleinige Verantwortung.

Research paper thumbnail of Functional diversity of human mitochondrial J-proteins is independent of their association with the inner membrane presequence translocase

The Journal of biological chemistry, Jan 21, 2016

Mitochondrial J-proteins play a critical role in governing Hsp70 activity and hence, are essentia... more Mitochondrial J-proteins play a critical role in governing Hsp70 activity and hence, are essential for organellar protein translocation and folding. In contrast to yeast which has a single J-protein Pam18, humans involve two J-proteins DnaJC15 and DnaJC19, associated with contrasting cellular phenotype, to transport proteins into the mitochondria. Mutation in DnaJC19 results in Dilated Cardiomyopathy and Ataxia syndrome (DCM) whereas expression of DnaJC15 regulates the response of cancer cells to chemotherapy. In the present study, we have comparatively assessed the biochemical properties of the J-protein paralogs in relation to their association with the import channel. Both DnaJC15 and DnaJC19 formed two distinct subcomplexes with Magmas at the import channel. Knock-down analysis suggested an essential role for Magmas and DnaJC19 in organellar protein translocation and mitochondria biogenesis whereas DnaJC15 had dispensable supportive function. The J-proteins were found to have eq...

Research paper thumbnail of Mapping Key Residues of ISD11 Critical for NFS1-ISD11 Subcomplex Stability

Journal of Biological Chemistry, 2015

Research paper thumbnail of A Pd8 Tetrafacial Molecular Barrel as Carrier for Water Insoluble Fluorophore

Journal of the American Chemical Society, 2015

A new carbazole-based tetraimidazole ligand 1,3,6,8-tetra(1H-imidazol-1-yl)-9-methyl-9H-carbazole... more A new carbazole-based tetraimidazole ligand 1,3,6,8-tetra(1H-imidazol-1-yl)-9-methyl-9H-carbazole (L) has been synthesized. The unsymmetrical nature of L as well as the rotational freedom of imidazole donor moieties around C-N bond make it a special building unit, which upon treatment with cis-(tmeda)Pd(NO3)2 produced an unprecedented single linkage-isomeric Pd8 tetrafacial molecular nanobarrel (PSMBR-1) [tmeda = N,N,N',N'-tetramethylethane-1,2-diamine]. Unlike closed architectures, open barrel architecture of water-soluble PSMBR-1 makes it an ideal host for some water insoluble polyaromatic hydrocarbons in aqueous medium; one such inclusion complex coronene⊂PSMBR-1 was characterized by X-ray diffraction study. Moreover, the potential application of PSMBR-1 as carrier in aqueous medium for the transportation of water insoluble fluorophore (perylene) for live cell imaging is explored.

Research paper thumbnail of Highly Efficient Glutathione Peroxidase and Peroxiredoxin Mimetics Protect Mammalian Cells against Oxidative Damage

Angewandte Chemie, 2015

Novel isoselenazoles with high glutathione peroxidase (GPx) and peroxiredoxin (Prx) activities pr... more Novel isoselenazoles with high glutathione peroxidase (GPx) and peroxiredoxin (Prx) activities provide remarkable cytoprotection to human cells, mainly by exhibiting antioxidant activities in the presence of cellular thiols. The cytotoxicity of the isoselenazoles is found to be significantly lower than that of ebselen, which is being clinically evaluated by several groups for the treatment of reperfusion injuries and stroke, hearing loss, and bipolar disorder. The compounds reported in this paper have the potential to be used as therapeutic agents for disorders mediated by reactive oxygen species.

Research paper thumbnail of Vagotonic action of carbon dioxide

Indian journal of physiology and pharmacology, 1966

Research paper thumbnail of Role of metal resistant plant growth promoting bacteria in ameliorating fly ash to the growth of Brassica juncea

Journal of Hazardous Materials, 2009

In this study, we have shown that the plant growth promoting bacterial strain NBRI K24 and strain... more In this study, we have shown that the plant growth promoting bacterial strain NBRI K24 and strain NBRI K3 from fly ash (FA) contaminated soil reduce the toxicity of Ni and Cr in Brassica juncea (Indian mustard) and promote plant growth under pot culture experiments. Isolated strains NBRI K24 and NBRI K3 were characterized based on the 16S rDNA sequencing and identified as Enterobacter aerogenes and Rahnella aquatilis respectively. Both the strains were siderophore producing and found capable of stimulating plant biomass and enhance phytoextraction of metals (Ni and Cr) from FA by metal accumulating plant i.e. B. juncea. Concurrent production of siderophores, 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase, indole acetic acid (IAA) and phosphate solubilization revealed their plant growth promotion potential.

Research paper thumbnail of The Presence of Multiple Cellular Defects Associated with a Novel G50E Iron-Sulfur Cluster Scaffold Protein (ISCU) Mutation Leads to Development of Mitochondrial Myopathy

Journal of Biological Chemistry, 2014

Background: Muscle-specific deficiency of iron-sulfur (Fe-S) cluster scaffold protein (ISCU) lead... more Background: Muscle-specific deficiency of iron-sulfur (Fe-S) cluster scaffold protein (ISCU) leads to myopathy. Results: Cells carrying the myopathy-associated G50E ISCU mutation demonstrate impaired Fe-S cluster biogenesis and mitochondrial dysfunction. Conclusion: Reduced mitochondrial respiration as a result of diminished Fe-S cluster synthesis results in muscle weakness in myopathy patients. Significance: The molecular mechanism behind disease progression should provide invaluable information to combat ISCU myopathy.

Research paper thumbnail of Influence of plant growth promoting bacteria and Cr6+ on the growth of Indian mustard

Chemosphere, 2006

The Cr 6+ resistant plant growth promoting bacteria (PGPB), Pseudomonas sp. PsA4 and Bacillus sp.... more The Cr 6+ resistant plant growth promoting bacteria (PGPB), Pseudomonas sp. PsA4 and Bacillus sp. Ba32 were isolated from heavy metal contaminated soils and their plant growth promoting activity on the Indian mustard (Brassica juncea) were assessed with different concentrations of Cr 6+ in soil. Production of siderophores and the solubilization of phosphate were observed in both strains, PsA4 and Ba32. Production of IAA was only observed in strain PsA4. Inoculation of PsA4 or Ba32 promoted the growth of plants at 95.3 and 198.3 lg of Cr 6+ g À1 soil. The maximum growth was observed in the plants inoculated with strain PsA4. Both strains, PsA4 and Ba32 did not influence the quantity of accumulation of chromium in root and shoot system. The present observations showed that the strains PsA4 and Ba32 protect the plants against the inhibitory effects of chromium, probably due to the production of IAA, siderophores and solubilization of phosphate.

Research paper thumbnail of Mitigation approach of arsenic toxicity in chickpea grown in arsenic amended soil with arsenic tolerant plant growth promoting Acinetobacter sp

Ecological Engineering, 2014

ABSTRACT The study was undertaken with the aim of testing the effects of isolated bacterial strai... more ABSTRACT The study was undertaken with the aim of testing the effects of isolated bacterial strain nbri05 from arsenic contaminated site of West Bengal. Firstly, isolated strain was characterized by morphological, biochemical and functional characterization and was able to grow at higher concentration of arsenic ranging up to 50,000 mg l−1 of As(V) (arsenate) and 1500 mg l−1 of As(III) (arsenite) concentration. After complete characterization, pot experiment was conducted to scrutinize the role of isolated bacterium nbri05 on arsenic uptake, plant growth, biomass and some biochemical responses of chickpea (Cicer aritenum) were grown in arsenic treated soil amended with 10 mg kg−1 of sodium arsenate. It was observed that nbri05 combat the As toxicity in plants by reducing the As uptake in shoot part and significantly increases plant growth and yield of the chickpea plant in comparison to only arsenic treated plant. Thus, these results clearly show that the bacterium nbri05 owing to its intrinsic abilities of plant growth promotion and accumulates more As in root of the plant may be accounted as a new bacterium for phytostabilization in chickpea.

Research paper thumbnail of イネ根圏界面におけるひ素耐性細菌株のバイオレメディエーション可能性の評価【Powered by NICT】

Journal of Environmental Sciences-china, 2016

Research paper thumbnail of Marf-mediated mitochondrial fusion is imperative for the development and functioning of indirect flight muscles (IFMs) in drosophila

Experimental Cell Research, 2021

Dynamic changes in mitochondrial shape and size are vital for mitochondrial health and for tissue... more Dynamic changes in mitochondrial shape and size are vital for mitochondrial health and for tissue development and function. Adult Drosophila indirect flight muscles contain densely packed mitochondria. We show here that mitochondrial fusion is critical during early muscle development (in pupa) and that silencing of the outer mitochondrial membrane fusion gene, Marf, in muscles results in smaller mitochondria that are functionally defective. This leads to abnormal muscle development resulting in muscle dysfunction in adult flies. However, post-developmental silencing of Marf has no obvious effects on mitochondrial and muscle phenotype in adult flies, indicating the importance of mitochondrial fusion during early muscle development.

Research paper thumbnail of Evolving paradigms on the interplay of mitochondrial Hsp70 chaperone system in cell survival and senescence

Critical Reviews in Biochemistry and Molecular Biology, 2019

The role of mitochondria within a cell has grown beyond being the prime source of cellular energy... more The role of mitochondria within a cell has grown beyond being the prime source of cellular energy to one of the major signaling platforms. Recent evidence provides several insights into the crucial roles of mitochondrial chaperones in regulating the organellar response to external triggers. The mitochondrial Hsp70 (mtHsp70/Mortalin/Grp75) chaperone system plays a critical role in the maintenance of proteostasis balance in the organelle. Defects in mtHsp70 network result in attenuated protein transport and misfolding of polypeptides leading to mitochondrial dysfunction. The functions of Hsp70 are primarily governed by J-protein cochaperones. Although human mitochondria possess a single Hsp70, its multifunctionality is characterized by the presence of multiple specific J-proteins. Several studies have shown a potential association of Hsp70 and J-proteins with diverse pathological states that are not limited to their canonical role as chaperones. The role of mitochondrial Hsp70 and its co-chaperones in disease pathogenesis has not been critically reviewed in recent years. We evaluated some of the cellular interfaces where Hsp70 machinery associated with pathophysiological conditions, particularly in context of tumorigenesis and neurodegeneration. The mitochondrial Hsp70 machinery shows a variable localization and integrates multiple components of the cellular processes with varied phenotypic consequences. Although Hsp70 and J-proteins function synergistically in proteins folding, their precise involvement in pathological conditions is mainly idiosyncratic. This machinery is associated with a heterogeneous set of molecules during the progression of a disorder. However, the precise binding to the substrate for a specific physiological response under a disease subtype is still an undocumented area of analysis.

Research paper thumbnail of A Redox Modulatory Mn3O4Nanozyme with Multi-Enzyme Activity Provides Efficient Cytoprotection to Human Cells in a Parkinson's Disease Model

Angewandte Chemie, 2017

Angabe der unten stehenden Digitalobjekt-Identifizierungsnummer (DOI) zitiert werden. Die deutsch... more Angabe der unten stehenden Digitalobjekt-Identifizierungsnummer (DOI) zitiert werden. Die deutsche Übersetzung wird gemeinsam mit der endgültigen englischen Fassung erscheinen. Die endgültige englische Fassung (Version of Record) wird ehestmöglich nach dem Redigieren und einem Korrekturgang als Early-View-Beitrag erscheinen und kann sich naturgemäß von der AA-Fassung unterscheiden. Leser sollten daher die endgültige Fassung, sobald sie veröffentlicht ist, verwenden. Für die AA-Fassung trägt der Autor die alleinige Verantwortung.

Research paper thumbnail of Functional diversity of human mitochondrial J-proteins is independent of their association with the inner membrane presequence translocase

The Journal of biological chemistry, Jan 21, 2016

Mitochondrial J-proteins play a critical role in governing Hsp70 activity and hence, are essentia... more Mitochondrial J-proteins play a critical role in governing Hsp70 activity and hence, are essential for organellar protein translocation and folding. In contrast to yeast which has a single J-protein Pam18, humans involve two J-proteins DnaJC15 and DnaJC19, associated with contrasting cellular phenotype, to transport proteins into the mitochondria. Mutation in DnaJC19 results in Dilated Cardiomyopathy and Ataxia syndrome (DCM) whereas expression of DnaJC15 regulates the response of cancer cells to chemotherapy. In the present study, we have comparatively assessed the biochemical properties of the J-protein paralogs in relation to their association with the import channel. Both DnaJC15 and DnaJC19 formed two distinct subcomplexes with Magmas at the import channel. Knock-down analysis suggested an essential role for Magmas and DnaJC19 in organellar protein translocation and mitochondria biogenesis whereas DnaJC15 had dispensable supportive function. The J-proteins were found to have eq...

Research paper thumbnail of Mapping Key Residues of ISD11 Critical for NFS1-ISD11 Subcomplex Stability

Journal of Biological Chemistry, 2015

Research paper thumbnail of A Pd8 Tetrafacial Molecular Barrel as Carrier for Water Insoluble Fluorophore

Journal of the American Chemical Society, 2015

A new carbazole-based tetraimidazole ligand 1,3,6,8-tetra(1H-imidazol-1-yl)-9-methyl-9H-carbazole... more A new carbazole-based tetraimidazole ligand 1,3,6,8-tetra(1H-imidazol-1-yl)-9-methyl-9H-carbazole (L) has been synthesized. The unsymmetrical nature of L as well as the rotational freedom of imidazole donor moieties around C-N bond make it a special building unit, which upon treatment with cis-(tmeda)Pd(NO3)2 produced an unprecedented single linkage-isomeric Pd8 tetrafacial molecular nanobarrel (PSMBR-1) [tmeda = N,N,N',N'-tetramethylethane-1,2-diamine]. Unlike closed architectures, open barrel architecture of water-soluble PSMBR-1 makes it an ideal host for some water insoluble polyaromatic hydrocarbons in aqueous medium; one such inclusion complex coronene⊂PSMBR-1 was characterized by X-ray diffraction study. Moreover, the potential application of PSMBR-1 as carrier in aqueous medium for the transportation of water insoluble fluorophore (perylene) for live cell imaging is explored.

Research paper thumbnail of Highly Efficient Glutathione Peroxidase and Peroxiredoxin Mimetics Protect Mammalian Cells against Oxidative Damage

Angewandte Chemie, 2015

Novel isoselenazoles with high glutathione peroxidase (GPx) and peroxiredoxin (Prx) activities pr... more Novel isoselenazoles with high glutathione peroxidase (GPx) and peroxiredoxin (Prx) activities provide remarkable cytoprotection to human cells, mainly by exhibiting antioxidant activities in the presence of cellular thiols. The cytotoxicity of the isoselenazoles is found to be significantly lower than that of ebselen, which is being clinically evaluated by several groups for the treatment of reperfusion injuries and stroke, hearing loss, and bipolar disorder. The compounds reported in this paper have the potential to be used as therapeutic agents for disorders mediated by reactive oxygen species.

Research paper thumbnail of Vagotonic action of carbon dioxide

Indian journal of physiology and pharmacology, 1966

Research paper thumbnail of Role of metal resistant plant growth promoting bacteria in ameliorating fly ash to the growth of Brassica juncea

Journal of Hazardous Materials, 2009

In this study, we have shown that the plant growth promoting bacterial strain NBRI K24 and strain... more In this study, we have shown that the plant growth promoting bacterial strain NBRI K24 and strain NBRI K3 from fly ash (FA) contaminated soil reduce the toxicity of Ni and Cr in Brassica juncea (Indian mustard) and promote plant growth under pot culture experiments. Isolated strains NBRI K24 and NBRI K3 were characterized based on the 16S rDNA sequencing and identified as Enterobacter aerogenes and Rahnella aquatilis respectively. Both the strains were siderophore producing and found capable of stimulating plant biomass and enhance phytoextraction of metals (Ni and Cr) from FA by metal accumulating plant i.e. B. juncea. Concurrent production of siderophores, 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase, indole acetic acid (IAA) and phosphate solubilization revealed their plant growth promotion potential.

Research paper thumbnail of The Presence of Multiple Cellular Defects Associated with a Novel G50E Iron-Sulfur Cluster Scaffold Protein (ISCU) Mutation Leads to Development of Mitochondrial Myopathy

Journal of Biological Chemistry, 2014

Background: Muscle-specific deficiency of iron-sulfur (Fe-S) cluster scaffold protein (ISCU) lead... more Background: Muscle-specific deficiency of iron-sulfur (Fe-S) cluster scaffold protein (ISCU) leads to myopathy. Results: Cells carrying the myopathy-associated G50E ISCU mutation demonstrate impaired Fe-S cluster biogenesis and mitochondrial dysfunction. Conclusion: Reduced mitochondrial respiration as a result of diminished Fe-S cluster synthesis results in muscle weakness in myopathy patients. Significance: The molecular mechanism behind disease progression should provide invaluable information to combat ISCU myopathy.

Research paper thumbnail of Influence of plant growth promoting bacteria and Cr6+ on the growth of Indian mustard

Chemosphere, 2006

The Cr 6+ resistant plant growth promoting bacteria (PGPB), Pseudomonas sp. PsA4 and Bacillus sp.... more The Cr 6+ resistant plant growth promoting bacteria (PGPB), Pseudomonas sp. PsA4 and Bacillus sp. Ba32 were isolated from heavy metal contaminated soils and their plant growth promoting activity on the Indian mustard (Brassica juncea) were assessed with different concentrations of Cr 6+ in soil. Production of siderophores and the solubilization of phosphate were observed in both strains, PsA4 and Ba32. Production of IAA was only observed in strain PsA4. Inoculation of PsA4 or Ba32 promoted the growth of plants at 95.3 and 198.3 lg of Cr 6+ g À1 soil. The maximum growth was observed in the plants inoculated with strain PsA4. Both strains, PsA4 and Ba32 did not influence the quantity of accumulation of chromium in root and shoot system. The present observations showed that the strains PsA4 and Ba32 protect the plants against the inhibitory effects of chromium, probably due to the production of IAA, siderophores and solubilization of phosphate.

Research paper thumbnail of Mitigation approach of arsenic toxicity in chickpea grown in arsenic amended soil with arsenic tolerant plant growth promoting Acinetobacter sp

Ecological Engineering, 2014

ABSTRACT The study was undertaken with the aim of testing the effects of isolated bacterial strai... more ABSTRACT The study was undertaken with the aim of testing the effects of isolated bacterial strain nbri05 from arsenic contaminated site of West Bengal. Firstly, isolated strain was characterized by morphological, biochemical and functional characterization and was able to grow at higher concentration of arsenic ranging up to 50,000 mg l−1 of As(V) (arsenate) and 1500 mg l−1 of As(III) (arsenite) concentration. After complete characterization, pot experiment was conducted to scrutinize the role of isolated bacterium nbri05 on arsenic uptake, plant growth, biomass and some biochemical responses of chickpea (Cicer aritenum) were grown in arsenic treated soil amended with 10 mg kg−1 of sodium arsenate. It was observed that nbri05 combat the As toxicity in plants by reducing the As uptake in shoot part and significantly increases plant growth and yield of the chickpea plant in comparison to only arsenic treated plant. Thus, these results clearly show that the bacterium nbri05 owing to its intrinsic abilities of plant growth promotion and accumulates more As in root of the plant may be accounted as a new bacterium for phytostabilization in chickpea.