Sudirman Numba - Academia.edu (original) (raw)
Papers by Sudirman Numba
Biodiversitas Journal of Biological Diversity
Numba S. 2023. Genetic diversity and its association with Phytophthora palmivora resistance in du... more Numba S. 2023. Genetic diversity and its association with Phytophthora palmivora resistance in durian (Durio zibethinus) using RAPD markers. Biodiversitas 24: 4542-4548. Durian (Durio zibethinus L.) is a popular tropical fruit that contributes significantly to regional economic value. Root and stem rot disease caused by Phytophthora palmivora has become a serious threat to the growth and development of durian. Indonesia has various local durian varieties that have the potential of resistant to P. palmivora. This study aims to analyze genetic diversity and its association with the resistance of durian to root and stem rot disease caused by P. palmivora using RAPD markers. Ten varieties of durian were compared with those of 2 wild species, namely D. kutejensis (Hassk.) Becc. and Boschia excelsa Korth.. The DNA profile analysis was performed by using 10 RAPD primers. Furthermore, cluster analysis was done using the NTSYS (Numerical Taxonomic and Multivariate Analysis System) ver. 2.0. Observation of the DNA profile (DNA banding pattern) showed that all the primers succeeded in producing amplified DNA fragments. Of the total 78 bands produced, 10 bands were considered monomorphic, while 68 bands (87.20%) were considered polymorphic. The high level of polymorphism causes a low level of similarity between varieties so that not all resistant varieties are grouped in the same cluster. This study found that the 1kb band produced by the OPA-2 primer was a potential gen associated with resistance to P. palmivora. It is suggested to carry out further tests on crosses of resistant and susceptible varieties.
AGROTEK: Jurnal Ilmiah Ilmu Pertanian
Sweet corn is a type of corn that has special characteristics because of its high sugar content s... more Sweet corn is a type of corn that has special characteristics because of its high sugar content so it can be used in various types of food, including snacks, various kinds of vegetables, corn sugar, and so on. Based on this, the demand for sweet corn is increasing so efforts are needed to increase production by increasing the correct population density per unit area and using the right type of manure. This study aims to get the population density and the right type of manure to increase the growth and production of sweet corn plants. This research was conducted in Lera Village, Wotu District, East Luwu Regency, South Sulawesi Province, starting in July-October 2019. This research used a factorial of 2-factor of the randomized block design method. The Factor I: population density (P) consists of 3 levels, namely: P1 (one plant per clump), P2 (two plants per clump), P3 (three plants per clump), and factor II is the type of manure (A) with 3 types, namely: A1 (chicken manure 15 t/ha), ...
ABSTRAKHasrun Hafid. Analisis Mutu biji kakao fermentasi pada beberapa variasi wadah dan tinggi t... more ABSTRAKHasrun Hafid. Analisis Mutu biji kakao fermentasi pada beberapa variasi wadah dan tinggi tumpukan dibimbing oleh Sudirman Numba dan Ayu Kartini Parawansa.Penelitian ini dilakukan dengan tujuan untuk : (1) mencari wadah fermentasi yang sesuai untuk melakukan fermentasi biji kakao; (2) Menganalisis tinggi tumpukan biji kakao yang optimum dengan wadah yang berbeda yang dapat mempengaruhi mutu biji kakao.Penelitian ini menggunakan rancangan tersarang ulangan sama yang terdiri atas dua faktor yaitu faktor A (wadah) yang terdiri atas 2 jenis wadah yaitu wadah keranjang dan kotak dan faktor B (tinggi tumpukan) yang terdiri atas 3 tingkatan yaitu 40 cm, 50 cm dan 60 cm, serta di ulang sebanyak 3 kali. Penelitian ini dilakukan dari bulan Juli s.d September 2014 di Desa Batualang, Kabupatean Luwu utara, Sulawesi Selatan. Data yang diperoleh dianalisis dengan Uji t dan analisis ragam one way ANOVA. Apabila terjadi perbedaan yang nyata/signifikan dilanjutkan dengan Uji multiple comparati...
AGrotekMAS Jurnal Indonesia: Jurnal Ilmu Pertanian, 2020
This study aims that knowing the level of resistance of several rice varieties to stem borer in o... more This study aims that knowing the level of resistance of several rice varieties to stem borer in organic and inorganic input paddy ecosystems. The research was conducted in the rice fields of Nonong Village, Batui District, Banggai Regency, from March to June 2020. The research used the Split Plot Design method. The main plot was the rice field ecosystem, namely organic input rice fields and inorganic input rice fields, while the subplots were 4 types of rice varieties, namely: Ciherang, Cisantana, Ciliwung and Towuti. Each treatment unit was repeated three times in order to obtain 24 experimental units. The results showed that the towuti variety planted in the paddy field ecosystem with inorganic input was more resistant to rice stem borer. The intensity of attack and the population of stem borer in the lowland organic matter input ecosystem was higher than the inorganic input lowland ecosystem. There is no interaction between rice varieties and types of paddy ecosystems (input of o...
IOP Conference Series: Earth and Environmental Science, 2018
National awareness for Halal Certificate is not currently experiencing significant development. S... more National awareness for Halal Certificate is not currently experiencing significant development. Stagnation that occurred in the validation rules on halal assurance in Indonesia is need the national movement that the people of Indonesia should quickly get protection from the government will be the fulfillment of their rights as citizens, focusing on the rules of halal food, drugs and cosmetics that used. Halal awareness is affected by several factors such as halal knowledge with frequent follow halal development or halal exhibition event, or halal training and the like, also the time factor of halal certification process. This paper aims to determine the factors that influence awareness of halal products in society. The conclusions are can be formulated so create awareness of halal that the best solutions can be realized on halal consciousness is by designing a system that is able to formulate basic halal food security for all people so that they feel that products marketed in Indonesia is a product that is guaranteed safe, healthy and halal.
Agrivigor, 2010
ABSTRACT The specific primer can be used for identification intra and interspecific somatic hybri... more ABSTRACT The specific primer can be used for identification intra and interspecific somatic hybridization potato has been by using RAPD method. The results of research showed that of 30 primers were used in the analysis of genetic diversity, there were eight primers that could be used to identify plants that produced somatic hybridization between BF-15, and Nicola, five primers for BF-15 and Aminca, six primers for BF-15 and Cardinal, three primers for the Cardinal, and Nicola, 12primers for the BF-15 and S.phureja , and 16 primers for the BF-15 and S , stenotomum . It also showed that there were a number of primers that could be used to identify plants that those were not able to be regenerated yet, i.e. 22 primers for the BF-15 and S.verney , 18 primers for BF-15 and S.chacoense , 18 primers for the BF-15 and S. microdontum , as well as 16 primers for the BF-15 and S. sparsipilum . Of the total primers, the primer of OPA-10 and OPA-16 apparently could be used to identify all the intra and interspecific somatic hybridization potatoes. Keywords : specific primay, somatic hybridization,intra and interspecific.
AGROTEK: Jurnal Ilmiah Ilmu Pertanian, 2018
Red Chilli pepper is one kind of plant that has high economic value, because it is necessary not ... more Red Chilli pepper is one kind of plant that has high economic value, because it is necessary not only for household needs, but also a need for the industry. This research was conducted at the location of Farmers Kelompok Tani Sumber Rezeki, Toddopulia Village, District Tanralili, Maros, which lasted from March to July 2016. The study aims to analyze the effect of pruning on the growth and yield of different varieties of chilli red. This study was designed by Split Plot Design. In the main plot pruning consists of two levels ie without trimming and pruning wild Shoots, as a subplot that varieties of three types: Princes variety, variety Kastilo F1variety, and Monser F1 variety. The results showed that pruning does not affect the component plant height and the number of productive branches, but a good effect on crop production with a number of fruit crops gained 135.68 fruit, fruit weight per plant was obtained 635.10 g of fruit weight per plot was obtained 10, 85 kg. Weight of fruit per hectare high of 30.15 tons obtained in the interaction between pruning and varietys Princes.
AGROTEK: Jurnal Ilmiah Ilmu Pertanian, 2017
Segregation pattern of the chloroplast genome in somatic hybridization potato between S. tuberosu... more Segregation pattern of the chloroplast genome in somatic hybridization potato between S. tuberosum cv. BF-15 and the wild species of S. stenotomum was identified through RFLP analysis in 3.2 kb fragment produced from PCR amplification for specific regions of DNA chloroplast. PCR amplification was performed by using rbcL primer and ORF106, i.e. specific primer located on konsenrvatiive sequence which flanking 3.2 kb fragment region of DNA chloroplast. PCR amplification product used two primers, in conformity with the target region on DNA chloroplast, where generated fragments or DNA bands with a size of about 3.2 kb. The 3.2 kb fragment produced from amplification was cut by using two kinds of restriction enzymes HhaI and RSAI. Restriction Enzym treatment with HhaI resulted in four bands each measuring; those were 2.0 kb, 1.2 kb, 0.8 kb and 0.4 kb. While the treatments by using RSAI restriction enzymes, also resulted in four bands each measuring, those were 1.6 kb, 0.7 kb, 0.3 kb and 0.2 kb. Band pattern that produced from restriction enzyme showed that monomorphic nature or not polymorphic at all elder fusion plant, so this method can not be used to identify the patterns of chloroplast genome segregation in plant which is produced by somatic hybridization.
Agrivigor, 2010
The use of reliability of Random Amplified Polymorphic DNA (RAPD) method in analysis of genetic d... more The use of reliability of Random Amplified Polymorphic DNA (RAPD) method in analysis of genetic diversity has been employed in six potato cultivars and its wild species. A total of 30 random primers were used to produce 230 polymorphic bands. The results of research showed that there were a number of primers that could come up with specific bands on potato cultivars BF-15, Nicola, Cardinal, Aminca, SPV-10, Katahdin, and the wild species of S. phureja and S. stenotomum , but it was not found in other wild species (S. chacoense, S. sparsipilum, S. microdontum and S. verney). It also showed that there were a number of primers that could come up with specific bands in each of wild potatoes species but it was not found in the potato cultived. Clustering analysis using Unweighted Pair-Group Method arithmetic (UPGMA) showed that cultived potatoes clustering made group itself in the degree of similarity around 70 percent, and significantly similar with S. stenotomum and S. phureja than other wild species. Keywords : Genetic diversity, Potatoes, Random Amplified Polymorphic DNA (RAPD)
IOP Conference Series: Earth and Environmental Science, 2021
Quality seeds are one of the important elements in maintaining plant potential. One of the factor... more Quality seeds are one of the important elements in maintaining plant potential. One of the factors that can affect the growth potential and production of corn for each variety or genotype is the storage ages of the seeds. The purpose of this study was to obtain varieties or genotypes of corn that have the ability to grow and produce optimally from seeds that have been stored for one and six months. The research was conducted from August to December 2020 at the Cereal Research Institute, Maros, South Sulawesi, Indonesia. The seeds used were Variety of Srikandi Putih, Waxy Corn and Genotype of BC2F1. This study was designed using a randomized block design (RBD) with a two-factor. The first factor is the type of variety/genotype (V) consisting of Srikandi Putih (V1), Waxy Corn (V2) and Genotype of BC2F1 (V3). The second factor is seed storage ages (U) consisting of: 1 month (U1) and 6 months (U2). From these two factors, 6 treatment combinations were obtained which were repeated three ...
AGROTEK: Jurnal Ilmiah Ilmu Pertanian, 2018
This study aims to analyze the response of various genotypes of early maturity at various plant s... more This study aims to analyze the response of various genotypes of early maturity at various plant spacing. The study was conducted from May to July 2017.The study was designed following a split-plot design pattern.As the main plot is the genotype of maize consisting of four genotypes G1, G2, G3, and G4.As a subplot is a spacing arrangement consisting of three levelnamely: sapacing of 70 cm x 20 cm (J1), spacing of 60 cm x 20 cm (J2) and spacing of 50 cm x 20 cm (J3).The results showed that the growth and production of maize was affected by the genotype and spacing used. The maize genotypes G3 and G4 resulted in higher plants with a closer spacing (60 x 20 cm and 50 x 20 cm) than with wider plant spacing (70 x 20 cm). In planting distance treatment showed that plant spacing of 60 x 20 cm and 50 x 20 obtained significantly higher production that is between 8.95 to 9.35 t / ha compared with the spacing of 70 x 20 cm which produces only 7.64 t /Ha.
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy), 2018
ABSTRACTMaize is commonly developed in dry land areas with limited irrigation water in Indonesia,... more ABSTRACTMaize is commonly developed in dry land areas with limited irrigation water in Indonesia, thereby posing a threat to its poductivity. Thus, availability of early harvest varieties able to adapt to limited water is important. The aim of this study was to analyze the performance of maize hybrid genotypes candidates in dry land. The research was conducted from April to July 2016 at Gowa, South Sulawesi. The experiment was designed with a randomized complete block design consisting of 8 genotypes, namely GJ1, GJ2, GJ3, GJ4, GJ5, GJ6, GJ7, GJ8, and two control varieties, i.e., Bima 7 and Lamuru. The results showed genotypes GJ1, GJ6, GJ7 and GJ8 had higher plant growth as compared to Bima 7 and Lamuru. All genotypes were harvested less than 90 days. Genotypes GJ6, GJ7, and GJ8 yielded 6.85 tons ha-1, 6.51 tons ha-1 and 8.31 tons ha-1, respectively, which were higher 13-45% than the Lamuru control variety. Because this experiment was carried out in an optimun condition, it ...
IOP Conference Series: Earth and Environmental Science, 2020
AGROTEK: Jurnal Ilmiah Ilmu Pertanian, 2020
Land use does not the rules of soil and water conservation potentially lead to land causes of deg... more Land use does not the rules of soil and water conservation potentially lead to land causes of degradation that will eventually lead to critical land. The impact ofthe existence of critical landis the decrease of soil fertility, lack of water resoucesin dryseasonandin rain season. Critical land mapping is necessary to determine the right efforts in the management of upper course of Binanga Lantang Sub Watershed until not distrub ecosystem balanced.This study aims to identify the extent and distribution of critical land and formulate appropriate land use directions to suppress degraded land in Binanga Lantang Sub Watershed. The method based on Forestry Departement P.4/V-SET/2013 by overlap method, scoring and weighting of each parameter. The parameters used are map of vegetation class, productivity class map, class map slope, erosion class map and class map management. Reseach results show (1) Classification of critical land after land use direction Binanga Lantang Sub Watershed i...
AGROTEK: Jurnal Ilmiah Ilmu Pertanian, 2019
The study of degraded areas n the pattern of ecosystem restoration in the wildlife comma area of ... more The study of degraded areas n the pattern of ecosystem restoration in the wildlife comma area of the Takalar district is a study that examines the location of degraded sites in wildlife reserves before ecosystem restoratioan activities in the area. This study aims to identify the extent of degraded sites, know the type of damage that occurs in a degraded location and determine the recovery pattern to be implemented. The area of degradation in the wildlife reserve in Takalar district is 298.84 ha, from the results of visual identification/image in 2016 then conducted direct measurement of wildlife sanctuary Ko'mara with an area of 122.65 ha. Determination of the type of damage to the degraded site is done by inventory of stand to determine the number of nature regeneration/seedlings, stable diameter 10 cm to 20 cm and stands ≥ 20 cm in diameter. The result of the stock inventory shows the type of damage to the degraded location in the Ko'mara wildlife reserve of Takalar district is moderately damaged with plant enrichment recovery pattern.
AGROTEK: Jurnal Ilmiah Ilmu Pertanian, 2019
The Strategy of Social Forest Management in Sinjai Regency was examined using SWOT and AHP method... more The Strategy of Social Forest Management in Sinjai Regency was examined using SWOT and AHP methods. This method is carried out by collecting information on internal factors (strengths and weaknesses) and external (opportunities and threats) of respondents consisting of Forest Comunity, Head of Forestry Division of Environment and Forestry OfficeSinjai Regency, the village head involved in the community forest program, & the Forestry Counselor of Sinjai Regency. Based on this information in the development of Social Forestry a strategy should be determined as an alternative strategy that must be applied. To determine the best alternative that will be applied in accordance with the purpose then used the incorporation of SWOT and AHP methods to compare the existing alternatives. The Social Forest Management Strategy of Sinjai Regency based on its priorities consists of: (1) Strengthening the role of the group by providing accurate market information (2) Conducting forestry and guidance from the government for the management of the area and the formulation of the management plan. (3) The Government facilitates the licensing to establish a timber industry to increase the value of timber and (4) using agroforestry System.
Biodiversitas Journal of Biological Diversity
Numba S. 2023. Genetic diversity and its association with Phytophthora palmivora resistance in du... more Numba S. 2023. Genetic diversity and its association with Phytophthora palmivora resistance in durian (Durio zibethinus) using RAPD markers. Biodiversitas 24: 4542-4548. Durian (Durio zibethinus L.) is a popular tropical fruit that contributes significantly to regional economic value. Root and stem rot disease caused by Phytophthora palmivora has become a serious threat to the growth and development of durian. Indonesia has various local durian varieties that have the potential of resistant to P. palmivora. This study aims to analyze genetic diversity and its association with the resistance of durian to root and stem rot disease caused by P. palmivora using RAPD markers. Ten varieties of durian were compared with those of 2 wild species, namely D. kutejensis (Hassk.) Becc. and Boschia excelsa Korth.. The DNA profile analysis was performed by using 10 RAPD primers. Furthermore, cluster analysis was done using the NTSYS (Numerical Taxonomic and Multivariate Analysis System) ver. 2.0. Observation of the DNA profile (DNA banding pattern) showed that all the primers succeeded in producing amplified DNA fragments. Of the total 78 bands produced, 10 bands were considered monomorphic, while 68 bands (87.20%) were considered polymorphic. The high level of polymorphism causes a low level of similarity between varieties so that not all resistant varieties are grouped in the same cluster. This study found that the 1kb band produced by the OPA-2 primer was a potential gen associated with resistance to P. palmivora. It is suggested to carry out further tests on crosses of resistant and susceptible varieties.
AGROTEK: Jurnal Ilmiah Ilmu Pertanian
Sweet corn is a type of corn that has special characteristics because of its high sugar content s... more Sweet corn is a type of corn that has special characteristics because of its high sugar content so it can be used in various types of food, including snacks, various kinds of vegetables, corn sugar, and so on. Based on this, the demand for sweet corn is increasing so efforts are needed to increase production by increasing the correct population density per unit area and using the right type of manure. This study aims to get the population density and the right type of manure to increase the growth and production of sweet corn plants. This research was conducted in Lera Village, Wotu District, East Luwu Regency, South Sulawesi Province, starting in July-October 2019. This research used a factorial of 2-factor of the randomized block design method. The Factor I: population density (P) consists of 3 levels, namely: P1 (one plant per clump), P2 (two plants per clump), P3 (three plants per clump), and factor II is the type of manure (A) with 3 types, namely: A1 (chicken manure 15 t/ha), ...
ABSTRAKHasrun Hafid. Analisis Mutu biji kakao fermentasi pada beberapa variasi wadah dan tinggi t... more ABSTRAKHasrun Hafid. Analisis Mutu biji kakao fermentasi pada beberapa variasi wadah dan tinggi tumpukan dibimbing oleh Sudirman Numba dan Ayu Kartini Parawansa.Penelitian ini dilakukan dengan tujuan untuk : (1) mencari wadah fermentasi yang sesuai untuk melakukan fermentasi biji kakao; (2) Menganalisis tinggi tumpukan biji kakao yang optimum dengan wadah yang berbeda yang dapat mempengaruhi mutu biji kakao.Penelitian ini menggunakan rancangan tersarang ulangan sama yang terdiri atas dua faktor yaitu faktor A (wadah) yang terdiri atas 2 jenis wadah yaitu wadah keranjang dan kotak dan faktor B (tinggi tumpukan) yang terdiri atas 3 tingkatan yaitu 40 cm, 50 cm dan 60 cm, serta di ulang sebanyak 3 kali. Penelitian ini dilakukan dari bulan Juli s.d September 2014 di Desa Batualang, Kabupatean Luwu utara, Sulawesi Selatan. Data yang diperoleh dianalisis dengan Uji t dan analisis ragam one way ANOVA. Apabila terjadi perbedaan yang nyata/signifikan dilanjutkan dengan Uji multiple comparati...
AGrotekMAS Jurnal Indonesia: Jurnal Ilmu Pertanian, 2020
This study aims that knowing the level of resistance of several rice varieties to stem borer in o... more This study aims that knowing the level of resistance of several rice varieties to stem borer in organic and inorganic input paddy ecosystems. The research was conducted in the rice fields of Nonong Village, Batui District, Banggai Regency, from March to June 2020. The research used the Split Plot Design method. The main plot was the rice field ecosystem, namely organic input rice fields and inorganic input rice fields, while the subplots were 4 types of rice varieties, namely: Ciherang, Cisantana, Ciliwung and Towuti. Each treatment unit was repeated three times in order to obtain 24 experimental units. The results showed that the towuti variety planted in the paddy field ecosystem with inorganic input was more resistant to rice stem borer. The intensity of attack and the population of stem borer in the lowland organic matter input ecosystem was higher than the inorganic input lowland ecosystem. There is no interaction between rice varieties and types of paddy ecosystems (input of o...
IOP Conference Series: Earth and Environmental Science, 2018
National awareness for Halal Certificate is not currently experiencing significant development. S... more National awareness for Halal Certificate is not currently experiencing significant development. Stagnation that occurred in the validation rules on halal assurance in Indonesia is need the national movement that the people of Indonesia should quickly get protection from the government will be the fulfillment of their rights as citizens, focusing on the rules of halal food, drugs and cosmetics that used. Halal awareness is affected by several factors such as halal knowledge with frequent follow halal development or halal exhibition event, or halal training and the like, also the time factor of halal certification process. This paper aims to determine the factors that influence awareness of halal products in society. The conclusions are can be formulated so create awareness of halal that the best solutions can be realized on halal consciousness is by designing a system that is able to formulate basic halal food security for all people so that they feel that products marketed in Indonesia is a product that is guaranteed safe, healthy and halal.
Agrivigor, 2010
ABSTRACT The specific primer can be used for identification intra and interspecific somatic hybri... more ABSTRACT The specific primer can be used for identification intra and interspecific somatic hybridization potato has been by using RAPD method. The results of research showed that of 30 primers were used in the analysis of genetic diversity, there were eight primers that could be used to identify plants that produced somatic hybridization between BF-15, and Nicola, five primers for BF-15 and Aminca, six primers for BF-15 and Cardinal, three primers for the Cardinal, and Nicola, 12primers for the BF-15 and S.phureja , and 16 primers for the BF-15 and S , stenotomum . It also showed that there were a number of primers that could be used to identify plants that those were not able to be regenerated yet, i.e. 22 primers for the BF-15 and S.verney , 18 primers for BF-15 and S.chacoense , 18 primers for the BF-15 and S. microdontum , as well as 16 primers for the BF-15 and S. sparsipilum . Of the total primers, the primer of OPA-10 and OPA-16 apparently could be used to identify all the intra and interspecific somatic hybridization potatoes. Keywords : specific primay, somatic hybridization,intra and interspecific.
AGROTEK: Jurnal Ilmiah Ilmu Pertanian, 2018
Red Chilli pepper is one kind of plant that has high economic value, because it is necessary not ... more Red Chilli pepper is one kind of plant that has high economic value, because it is necessary not only for household needs, but also a need for the industry. This research was conducted at the location of Farmers Kelompok Tani Sumber Rezeki, Toddopulia Village, District Tanralili, Maros, which lasted from March to July 2016. The study aims to analyze the effect of pruning on the growth and yield of different varieties of chilli red. This study was designed by Split Plot Design. In the main plot pruning consists of two levels ie without trimming and pruning wild Shoots, as a subplot that varieties of three types: Princes variety, variety Kastilo F1variety, and Monser F1 variety. The results showed that pruning does not affect the component plant height and the number of productive branches, but a good effect on crop production with a number of fruit crops gained 135.68 fruit, fruit weight per plant was obtained 635.10 g of fruit weight per plot was obtained 10, 85 kg. Weight of fruit per hectare high of 30.15 tons obtained in the interaction between pruning and varietys Princes.
AGROTEK: Jurnal Ilmiah Ilmu Pertanian, 2017
Segregation pattern of the chloroplast genome in somatic hybridization potato between S. tuberosu... more Segregation pattern of the chloroplast genome in somatic hybridization potato between S. tuberosum cv. BF-15 and the wild species of S. stenotomum was identified through RFLP analysis in 3.2 kb fragment produced from PCR amplification for specific regions of DNA chloroplast. PCR amplification was performed by using rbcL primer and ORF106, i.e. specific primer located on konsenrvatiive sequence which flanking 3.2 kb fragment region of DNA chloroplast. PCR amplification product used two primers, in conformity with the target region on DNA chloroplast, where generated fragments or DNA bands with a size of about 3.2 kb. The 3.2 kb fragment produced from amplification was cut by using two kinds of restriction enzymes HhaI and RSAI. Restriction Enzym treatment with HhaI resulted in four bands each measuring; those were 2.0 kb, 1.2 kb, 0.8 kb and 0.4 kb. While the treatments by using RSAI restriction enzymes, also resulted in four bands each measuring, those were 1.6 kb, 0.7 kb, 0.3 kb and 0.2 kb. Band pattern that produced from restriction enzyme showed that monomorphic nature or not polymorphic at all elder fusion plant, so this method can not be used to identify the patterns of chloroplast genome segregation in plant which is produced by somatic hybridization.
Agrivigor, 2010
The use of reliability of Random Amplified Polymorphic DNA (RAPD) method in analysis of genetic d... more The use of reliability of Random Amplified Polymorphic DNA (RAPD) method in analysis of genetic diversity has been employed in six potato cultivars and its wild species. A total of 30 random primers were used to produce 230 polymorphic bands. The results of research showed that there were a number of primers that could come up with specific bands on potato cultivars BF-15, Nicola, Cardinal, Aminca, SPV-10, Katahdin, and the wild species of S. phureja and S. stenotomum , but it was not found in other wild species (S. chacoense, S. sparsipilum, S. microdontum and S. verney). It also showed that there were a number of primers that could come up with specific bands in each of wild potatoes species but it was not found in the potato cultived. Clustering analysis using Unweighted Pair-Group Method arithmetic (UPGMA) showed that cultived potatoes clustering made group itself in the degree of similarity around 70 percent, and significantly similar with S. stenotomum and S. phureja than other wild species. Keywords : Genetic diversity, Potatoes, Random Amplified Polymorphic DNA (RAPD)
IOP Conference Series: Earth and Environmental Science, 2021
Quality seeds are one of the important elements in maintaining plant potential. One of the factor... more Quality seeds are one of the important elements in maintaining plant potential. One of the factors that can affect the growth potential and production of corn for each variety or genotype is the storage ages of the seeds. The purpose of this study was to obtain varieties or genotypes of corn that have the ability to grow and produce optimally from seeds that have been stored for one and six months. The research was conducted from August to December 2020 at the Cereal Research Institute, Maros, South Sulawesi, Indonesia. The seeds used were Variety of Srikandi Putih, Waxy Corn and Genotype of BC2F1. This study was designed using a randomized block design (RBD) with a two-factor. The first factor is the type of variety/genotype (V) consisting of Srikandi Putih (V1), Waxy Corn (V2) and Genotype of BC2F1 (V3). The second factor is seed storage ages (U) consisting of: 1 month (U1) and 6 months (U2). From these two factors, 6 treatment combinations were obtained which were repeated three ...
AGROTEK: Jurnal Ilmiah Ilmu Pertanian, 2018
This study aims to analyze the response of various genotypes of early maturity at various plant s... more This study aims to analyze the response of various genotypes of early maturity at various plant spacing. The study was conducted from May to July 2017.The study was designed following a split-plot design pattern.As the main plot is the genotype of maize consisting of four genotypes G1, G2, G3, and G4.As a subplot is a spacing arrangement consisting of three levelnamely: sapacing of 70 cm x 20 cm (J1), spacing of 60 cm x 20 cm (J2) and spacing of 50 cm x 20 cm (J3).The results showed that the growth and production of maize was affected by the genotype and spacing used. The maize genotypes G3 and G4 resulted in higher plants with a closer spacing (60 x 20 cm and 50 x 20 cm) than with wider plant spacing (70 x 20 cm). In planting distance treatment showed that plant spacing of 60 x 20 cm and 50 x 20 obtained significantly higher production that is between 8.95 to 9.35 t / ha compared with the spacing of 70 x 20 cm which produces only 7.64 t /Ha.
Jurnal Agronomi Indonesia (Indonesian Journal of Agronomy), 2018
ABSTRACTMaize is commonly developed in dry land areas with limited irrigation water in Indonesia,... more ABSTRACTMaize is commonly developed in dry land areas with limited irrigation water in Indonesia, thereby posing a threat to its poductivity. Thus, availability of early harvest varieties able to adapt to limited water is important. The aim of this study was to analyze the performance of maize hybrid genotypes candidates in dry land. The research was conducted from April to July 2016 at Gowa, South Sulawesi. The experiment was designed with a randomized complete block design consisting of 8 genotypes, namely GJ1, GJ2, GJ3, GJ4, GJ5, GJ6, GJ7, GJ8, and two control varieties, i.e., Bima 7 and Lamuru. The results showed genotypes GJ1, GJ6, GJ7 and GJ8 had higher plant growth as compared to Bima 7 and Lamuru. All genotypes were harvested less than 90 days. Genotypes GJ6, GJ7, and GJ8 yielded 6.85 tons ha-1, 6.51 tons ha-1 and 8.31 tons ha-1, respectively, which were higher 13-45% than the Lamuru control variety. Because this experiment was carried out in an optimun condition, it ...
IOP Conference Series: Earth and Environmental Science, 2020
AGROTEK: Jurnal Ilmiah Ilmu Pertanian, 2020
Land use does not the rules of soil and water conservation potentially lead to land causes of deg... more Land use does not the rules of soil and water conservation potentially lead to land causes of degradation that will eventually lead to critical land. The impact ofthe existence of critical landis the decrease of soil fertility, lack of water resoucesin dryseasonandin rain season. Critical land mapping is necessary to determine the right efforts in the management of upper course of Binanga Lantang Sub Watershed until not distrub ecosystem balanced.This study aims to identify the extent and distribution of critical land and formulate appropriate land use directions to suppress degraded land in Binanga Lantang Sub Watershed. The method based on Forestry Departement P.4/V-SET/2013 by overlap method, scoring and weighting of each parameter. The parameters used are map of vegetation class, productivity class map, class map slope, erosion class map and class map management. Reseach results show (1) Classification of critical land after land use direction Binanga Lantang Sub Watershed i...
AGROTEK: Jurnal Ilmiah Ilmu Pertanian, 2019
The study of degraded areas n the pattern of ecosystem restoration in the wildlife comma area of ... more The study of degraded areas n the pattern of ecosystem restoration in the wildlife comma area of the Takalar district is a study that examines the location of degraded sites in wildlife reserves before ecosystem restoratioan activities in the area. This study aims to identify the extent of degraded sites, know the type of damage that occurs in a degraded location and determine the recovery pattern to be implemented. The area of degradation in the wildlife reserve in Takalar district is 298.84 ha, from the results of visual identification/image in 2016 then conducted direct measurement of wildlife sanctuary Ko'mara with an area of 122.65 ha. Determination of the type of damage to the degraded site is done by inventory of stand to determine the number of nature regeneration/seedlings, stable diameter 10 cm to 20 cm and stands ≥ 20 cm in diameter. The result of the stock inventory shows the type of damage to the degraded location in the Ko'mara wildlife reserve of Takalar district is moderately damaged with plant enrichment recovery pattern.
AGROTEK: Jurnal Ilmiah Ilmu Pertanian, 2019
The Strategy of Social Forest Management in Sinjai Regency was examined using SWOT and AHP method... more The Strategy of Social Forest Management in Sinjai Regency was examined using SWOT and AHP methods. This method is carried out by collecting information on internal factors (strengths and weaknesses) and external (opportunities and threats) of respondents consisting of Forest Comunity, Head of Forestry Division of Environment and Forestry OfficeSinjai Regency, the village head involved in the community forest program, & the Forestry Counselor of Sinjai Regency. Based on this information in the development of Social Forestry a strategy should be determined as an alternative strategy that must be applied. To determine the best alternative that will be applied in accordance with the purpose then used the incorporation of SWOT and AHP methods to compare the existing alternatives. The Social Forest Management Strategy of Sinjai Regency based on its priorities consists of: (1) Strengthening the role of the group by providing accurate market information (2) Conducting forestry and guidance from the government for the management of the area and the formulation of the management plan. (3) The Government facilitates the licensing to establish a timber industry to increase the value of timber and (4) using agroforestry System.