Abdelaziz SOULI - Academia.edu (original) (raw)
Papers by Abdelaziz SOULI
Journal of Medicinal Plants Research, Jan 10, 2013
Http Www Theses Fr, 1997
Le peptide yy (pyy) est une hormone de 36 acides amines, synthetisee et liberee principalement pa... more Le peptide yy (pyy) est une hormone de 36 acides amines, synthetisee et liberee principalement par les cellules endocrines de type l de l'intestin distal. Ce peptide est structuralement proche du neuropeptide y (npy), tres abondant dans les neurones centraux et peripheriques, et a un moindre degre du polypeptide pancreatique (pp). Ces peptides inhibent la secretion d'eau et d'electrolytes dans l'intestin, mais les donnees de la litterature relatives au mecanisme de cet effet sont confuses. Le but de ce travail etait l'etude du mecanisme d'action du pyy sur la secretion d'eau dans l'intestin grele du rat in vivo. Dans ce but, nous avons principalement utilise la technique des anses intestinales ligaturees in situ chez le rat anesthesie. Nous avons mesure le flux net d'eau pendant une periode de 30 min, dans les conditions basales et sous stimulation par le peptide vasoactif intestinal (vip) ou par la prostaglandine e#2. Les etudes sur le jejunum de rat in vivo ont ete completees par des etudes effectuees sur le meme organe, in vitro en chambre d'ussing. Dans un travail preliminaire nous avons montre que les activites antisecretoires du pyy et du npy s'exercaient a differents niveaux de l'intestin grele : duodenum, jejunum et ileon, et que le pyy etait toujours plus puissant que le npy. L'etude du mecanisme d'action du pyy au niveau jejunal, effectuee en utilisant des antagonistes de neuromediateurs, a mis en evidence, in vivo, un mecanisme nerveux, impliquant des synapses nicotiniques, des recepteurs alpha#2-adrenergiques et des recepteurs sigma. La recherche des sous-types de recepteurs y mis en jeu dans cet effet antisecretoire in vivo a ete effectuee en comparant l'activite des peptides entiers a celle de fragments ou d'analogues connus pour leur activite plus specifiquement y#1 ou y#2. Les resultats suggerent que l'effet antisecretoire du pyy et du npy met en jeu plusieurs sous-types de recepteurs : le recepteur preferant le pyy, decrit sur les enterocytes de rat, un recepteur proche du recepteur y#1, et un recepteur presentant une forte affinite pour le pp. La comparaison des activites antisecretoires in vivo et des affinites de liaison in vitro montre un bon parallelisme pour le pyy et le npy entiers et pour les agonistes y#2, mais une divergence pour l'agoniste y#1, ainsi que pour le pp, tres faiblement reconnus par le recepteur preferant le pyy des cryptes enterocytaires de rat. L'effet inhibiteur du pyy sur la secretion electrogene de chlorure et son mecanisme nerveux ont ete confirmes sur le jejunum de rat en chambre d'ussing dans les conditions basales.
Journal of Agricultural and Food Chemistry, 2008
The present study assessed the chemical composition, antioxidant properties, and hepatoprotective... more The present study assessed the chemical composition, antioxidant properties, and hepatoprotective effects of subacute pre-treatment with chamomile (Matricaria recutita L.) decoction extract (CDE) against ethanol (EtOH)-induced oxidative stress in rats. The colorimetric analysis demonstrated that the CDE is rich in total polyphenols, total flavonoids and condensed tannins, and exhibited an important in vitro antioxidant activity. The use of LC/MS technique allowed us to identify 10 phenolic compounds in CDE. We found that CDE pretreatment, in vivo, protected against EtOH-induced liver injury evident by plasma transaminases activity and preservation of the hepatic tissue structure. The CDE counteracted EtOH-induced liver lipoperoxidation, preserved thiol-SH groups and prevented the depletion of antioxidant enzyme activity of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx). We also showed that acute alcohol administration increased tissue and plasma hydrogen peroxide (H 2 O 2), calcium and free iron levels. More importantly, CDE pre-treatment reversed all EtOH-induced disturbances in intracellular mediators. In conclusion, our data suggest that CDE exerted a potential hepatoprotective effect against EtOH-induced oxidative stress in rat, at least in part, by negatively regulating Fenton reaction components such as H 2 O 2 and free iron, which are known to lead to cytotoxicity mediated by intracellular calcium deregulation.
Gene, 1992
A rat lipoprotein lipase (LPL)-encoding cDNA (LPL) has been entirely sequenced and compared to th... more A rat lipoprotein lipase (LPL)-encoding cDNA (LPL) has been entirely sequenced and compared to the sequences of all the LPL cDNAs reported in other species. As expected, high homology was found between the coding exons. The putative catalytic triad, Ser'32, Aspire, His24', according to human numbering, is conserved in rat. As is the case in mouse, an Asn444 present in human LPL is also missing. The major divergences between human, mouse and rat LPLs were observed in the untranslated exon 10, where (i) the rat cDNA exhibits a 157-bp insertion and an 81-bp deletion relative to human; (ii) neither the B 1 repeat nor the homopurine stretch reported in mouse can be recognized, and (iii) the rat cDNA displays several A+T-rich stretches.
Tropical Animal Health and Production, Dec 13, 2023
Journal of Medicinal Food, Sep 1, 2015
General Physiology and Biophysics, 2015
Biological Rhythm Research
Antioxidants
Inflammation is the body’s response to insults, for instance, lung inflammation is generally caus... more Inflammation is the body’s response to insults, for instance, lung inflammation is generally caused by pathogens or by exposure to pollutants, irritants and toxins. This process involves many inflammatory cells such as epithelial cells, monocytes, macrophages and neutrophils. These cells produce and release inflammatory mediators such as pro-inflammatory cytokines, lipids and reactive oxygen species (ROS). Lung epithelial cells and phagocytes (monocytes, macrophages and neutrophils) produce ROS mainly by the NADPH oxidase NOX1 and NOX2, respectively. The aim of this study was to investigate the effects of two NADPH oxidase inhibitors, apocynin and diphenyleneiodonium (DPI), on lipopolysaccharide (LPS)-induced lung inflammation in rats. Our results showed that apocynin and DPI attenuated the LPS-induced morphological and histological alterations of the lung, reduced edema and decreased lung permeability. The evaluation of oxidative stress markers in lung homogenates showed that apocy...
Journal of New Sciences, 2020
Grasas y Aceites, 2020
The sea cucumber or holothurian is a marine species which has been prized in some Asian countrie... more The sea cucumber or holothurian is a marine species which has been prized in some Asian countries for its nutritional qualities. The purpose of this work was to study the biochemical composition and free radical scavenging and antioxidant activities of Holothuria tubulosa tegument from the Bizerta lagoon in northern Tunisia. The obtained data demonstrated that the extract of sea cucumber teguments exhibited high biochemical levels (such as moisture 80.77%, protein 7.07%, lipids 10.21%, energy value 13.64 Kcal/g ww), and an important nutritional value (including n-3/n-6: 2.11, EPA+DHA: 20.96, AI: 1.38 and TI: 0.54). High antioxidant activities were recorded in the integument by the radical scavenging tests of ABTS and DPPH as well as by the total antioxidant capacity and the FRAP in comparison with the BHT standard. Our results showed that H. Tubulosa tegument has high nutritional value with high antioxidant activities and could be considered a nutraceutical product.
Journal of Essential Oil Bearing Plants, 2018
South African Journal of Animal Science, 2017
BMC Complementary and Alternative Medicine, 2015
Background: We aimed in the present study, at investigating the gastroprotective effect of carob ... more Background: We aimed in the present study, at investigating the gastroprotective effect of carob pods aqueous extract (CPAE) against ethanol-induced oxidative stress in rats as well as the mechanism implicated. Methods: Adult male wistar rats were used and divided into six groups of ten each: control, EtOH (80 % v/v, 4 g/kg b.w.), EtOH 80 % + various doses of CPAE (500, 1000 and 2000 mg/kg, b.w.) and EtOH + Famotidine (10 mg/kg, p.o.) Animals were perorally (p.o.) pre-treated with CPAE during 15 days and intoxicated with a single oral administration of EtOH (4 g/kg b.w.) for two hours. Results: The colorimetric analysis demonstrated that the CPAE exhibited an importance in vitro antioxidant activity against ABTS and DPPH radicals. We found that CPAE pretreatment in vivo, protected against EtOH-induced macroscopic and histological changes induced in stomach mucosa. Carob extract administration also protected against alcohol-induced volume gastric juice decrease. More importantly, We showed that CPAE counteracted EtOH-induced gastric lipoperoxidation, reversed the decrease of sulfhydryl groups (−SH) an hydrogen peroxide (H 2 O 2) levels, and prevented the depletion of antioxidant enzyme activity of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx). Conclusions: These findings suggest that CPAE exerted a potential gastro-protective effect against EtOH-induced oxidative stress in rats, due in part, to its antioxidants properties.
RSC Advances, 2015
Chromatographic profiles of aqueous extract of carob pods ((A) pulp and (B) seeds).
RSC Advances, 2015
We aimed in the present study to investigate the protective effect of a myrtle (Myrtus communisL.... more We aimed in the present study to investigate the protective effect of a myrtle (Myrtus communisL.) berry seed aqueous extract (MBSAE) on acetic acid (AA)-induced colitis in rats as well as the mechanism implicated in this coli-protection.
Gastroentérologie clinique et biologique, 1996
The purpose of this study was to compare the effects of peptide YY (PYY) and neuropeptide Y (NPY)... more The purpose of this study was to compare the effects of peptide YY (PYY) and neuropeptide Y (NPY) on VIP- and PGE2-stimulated intestinal net water flux at three different levels of the small intestine (duodenum, jejunum, ileum), by a technique of in situ closed loops in anaesthetised rats. VIP-stimulated net water flux was efficiently inhibited by both peptides at all three intestinal levels studied; PYY (ID50 about 30 pmol/kg.h) was 3 to 18 fold more potent than NPY. PGE2-stimulated net water flux was also efficiently inhibited in the jejunum and ileum; PYY (ID50 about 10 pmol/kg.h) was 30 to 90 fold more potent than NPY. A 30% inhibition of PGE2-stimulated net water flux could only be achieved in the duodenum with the largest dose of either peptide used in this study. PYY and NPY display potent inhibitory effects of stimulated net water flux at the three studied levels of the small intestine, except in the PGE2-stimulated duodenum. The PYY ID50s measured suggest that PYY may have ...
Journal of Medicinal Food, 2015
Journal of Medicinal Plants Research, Jan 10, 2013
Http Www Theses Fr, 1997
Le peptide yy (pyy) est une hormone de 36 acides amines, synthetisee et liberee principalement pa... more Le peptide yy (pyy) est une hormone de 36 acides amines, synthetisee et liberee principalement par les cellules endocrines de type l de l'intestin distal. Ce peptide est structuralement proche du neuropeptide y (npy), tres abondant dans les neurones centraux et peripheriques, et a un moindre degre du polypeptide pancreatique (pp). Ces peptides inhibent la secretion d'eau et d'electrolytes dans l'intestin, mais les donnees de la litterature relatives au mecanisme de cet effet sont confuses. Le but de ce travail etait l'etude du mecanisme d'action du pyy sur la secretion d'eau dans l'intestin grele du rat in vivo. Dans ce but, nous avons principalement utilise la technique des anses intestinales ligaturees in situ chez le rat anesthesie. Nous avons mesure le flux net d'eau pendant une periode de 30 min, dans les conditions basales et sous stimulation par le peptide vasoactif intestinal (vip) ou par la prostaglandine e#2. Les etudes sur le jejunum de rat in vivo ont ete completees par des etudes effectuees sur le meme organe, in vitro en chambre d'ussing. Dans un travail preliminaire nous avons montre que les activites antisecretoires du pyy et du npy s'exercaient a differents niveaux de l'intestin grele : duodenum, jejunum et ileon, et que le pyy etait toujours plus puissant que le npy. L'etude du mecanisme d'action du pyy au niveau jejunal, effectuee en utilisant des antagonistes de neuromediateurs, a mis en evidence, in vivo, un mecanisme nerveux, impliquant des synapses nicotiniques, des recepteurs alpha#2-adrenergiques et des recepteurs sigma. La recherche des sous-types de recepteurs y mis en jeu dans cet effet antisecretoire in vivo a ete effectuee en comparant l'activite des peptides entiers a celle de fragments ou d'analogues connus pour leur activite plus specifiquement y#1 ou y#2. Les resultats suggerent que l'effet antisecretoire du pyy et du npy met en jeu plusieurs sous-types de recepteurs : le recepteur preferant le pyy, decrit sur les enterocytes de rat, un recepteur proche du recepteur y#1, et un recepteur presentant une forte affinite pour le pp. La comparaison des activites antisecretoires in vivo et des affinites de liaison in vitro montre un bon parallelisme pour le pyy et le npy entiers et pour les agonistes y#2, mais une divergence pour l'agoniste y#1, ainsi que pour le pp, tres faiblement reconnus par le recepteur preferant le pyy des cryptes enterocytaires de rat. L'effet inhibiteur du pyy sur la secretion electrogene de chlorure et son mecanisme nerveux ont ete confirmes sur le jejunum de rat en chambre d'ussing dans les conditions basales.
Journal of Agricultural and Food Chemistry, 2008
The present study assessed the chemical composition, antioxidant properties, and hepatoprotective... more The present study assessed the chemical composition, antioxidant properties, and hepatoprotective effects of subacute pre-treatment with chamomile (Matricaria recutita L.) decoction extract (CDE) against ethanol (EtOH)-induced oxidative stress in rats. The colorimetric analysis demonstrated that the CDE is rich in total polyphenols, total flavonoids and condensed tannins, and exhibited an important in vitro antioxidant activity. The use of LC/MS technique allowed us to identify 10 phenolic compounds in CDE. We found that CDE pretreatment, in vivo, protected against EtOH-induced liver injury evident by plasma transaminases activity and preservation of the hepatic tissue structure. The CDE counteracted EtOH-induced liver lipoperoxidation, preserved thiol-SH groups and prevented the depletion of antioxidant enzyme activity of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx). We also showed that acute alcohol administration increased tissue and plasma hydrogen peroxide (H 2 O 2), calcium and free iron levels. More importantly, CDE pre-treatment reversed all EtOH-induced disturbances in intracellular mediators. In conclusion, our data suggest that CDE exerted a potential hepatoprotective effect against EtOH-induced oxidative stress in rat, at least in part, by negatively regulating Fenton reaction components such as H 2 O 2 and free iron, which are known to lead to cytotoxicity mediated by intracellular calcium deregulation.
Gene, 1992
A rat lipoprotein lipase (LPL)-encoding cDNA (LPL) has been entirely sequenced and compared to th... more A rat lipoprotein lipase (LPL)-encoding cDNA (LPL) has been entirely sequenced and compared to the sequences of all the LPL cDNAs reported in other species. As expected, high homology was found between the coding exons. The putative catalytic triad, Ser'32, Aspire, His24', according to human numbering, is conserved in rat. As is the case in mouse, an Asn444 present in human LPL is also missing. The major divergences between human, mouse and rat LPLs were observed in the untranslated exon 10, where (i) the rat cDNA exhibits a 157-bp insertion and an 81-bp deletion relative to human; (ii) neither the B 1 repeat nor the homopurine stretch reported in mouse can be recognized, and (iii) the rat cDNA displays several A+T-rich stretches.
Tropical Animal Health and Production, Dec 13, 2023
Journal of Medicinal Food, Sep 1, 2015
General Physiology and Biophysics, 2015
Biological Rhythm Research
Antioxidants
Inflammation is the body’s response to insults, for instance, lung inflammation is generally caus... more Inflammation is the body’s response to insults, for instance, lung inflammation is generally caused by pathogens or by exposure to pollutants, irritants and toxins. This process involves many inflammatory cells such as epithelial cells, monocytes, macrophages and neutrophils. These cells produce and release inflammatory mediators such as pro-inflammatory cytokines, lipids and reactive oxygen species (ROS). Lung epithelial cells and phagocytes (monocytes, macrophages and neutrophils) produce ROS mainly by the NADPH oxidase NOX1 and NOX2, respectively. The aim of this study was to investigate the effects of two NADPH oxidase inhibitors, apocynin and diphenyleneiodonium (DPI), on lipopolysaccharide (LPS)-induced lung inflammation in rats. Our results showed that apocynin and DPI attenuated the LPS-induced morphological and histological alterations of the lung, reduced edema and decreased lung permeability. The evaluation of oxidative stress markers in lung homogenates showed that apocy...
Journal of New Sciences, 2020
Grasas y Aceites, 2020
The sea cucumber or holothurian is a marine species which has been prized in some Asian countrie... more The sea cucumber or holothurian is a marine species which has been prized in some Asian countries for its nutritional qualities. The purpose of this work was to study the biochemical composition and free radical scavenging and antioxidant activities of Holothuria tubulosa tegument from the Bizerta lagoon in northern Tunisia. The obtained data demonstrated that the extract of sea cucumber teguments exhibited high biochemical levels (such as moisture 80.77%, protein 7.07%, lipids 10.21%, energy value 13.64 Kcal/g ww), and an important nutritional value (including n-3/n-6: 2.11, EPA+DHA: 20.96, AI: 1.38 and TI: 0.54). High antioxidant activities were recorded in the integument by the radical scavenging tests of ABTS and DPPH as well as by the total antioxidant capacity and the FRAP in comparison with the BHT standard. Our results showed that H. Tubulosa tegument has high nutritional value with high antioxidant activities and could be considered a nutraceutical product.
Journal of Essential Oil Bearing Plants, 2018
South African Journal of Animal Science, 2017
BMC Complementary and Alternative Medicine, 2015
Background: We aimed in the present study, at investigating the gastroprotective effect of carob ... more Background: We aimed in the present study, at investigating the gastroprotective effect of carob pods aqueous extract (CPAE) against ethanol-induced oxidative stress in rats as well as the mechanism implicated. Methods: Adult male wistar rats were used and divided into six groups of ten each: control, EtOH (80 % v/v, 4 g/kg b.w.), EtOH 80 % + various doses of CPAE (500, 1000 and 2000 mg/kg, b.w.) and EtOH + Famotidine (10 mg/kg, p.o.) Animals were perorally (p.o.) pre-treated with CPAE during 15 days and intoxicated with a single oral administration of EtOH (4 g/kg b.w.) for two hours. Results: The colorimetric analysis demonstrated that the CPAE exhibited an importance in vitro antioxidant activity against ABTS and DPPH radicals. We found that CPAE pretreatment in vivo, protected against EtOH-induced macroscopic and histological changes induced in stomach mucosa. Carob extract administration also protected against alcohol-induced volume gastric juice decrease. More importantly, We showed that CPAE counteracted EtOH-induced gastric lipoperoxidation, reversed the decrease of sulfhydryl groups (−SH) an hydrogen peroxide (H 2 O 2) levels, and prevented the depletion of antioxidant enzyme activity of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPx). Conclusions: These findings suggest that CPAE exerted a potential gastro-protective effect against EtOH-induced oxidative stress in rats, due in part, to its antioxidants properties.
RSC Advances, 2015
Chromatographic profiles of aqueous extract of carob pods ((A) pulp and (B) seeds).
RSC Advances, 2015
We aimed in the present study to investigate the protective effect of a myrtle (Myrtus communisL.... more We aimed in the present study to investigate the protective effect of a myrtle (Myrtus communisL.) berry seed aqueous extract (MBSAE) on acetic acid (AA)-induced colitis in rats as well as the mechanism implicated in this coli-protection.
Gastroentérologie clinique et biologique, 1996
The purpose of this study was to compare the effects of peptide YY (PYY) and neuropeptide Y (NPY)... more The purpose of this study was to compare the effects of peptide YY (PYY) and neuropeptide Y (NPY) on VIP- and PGE2-stimulated intestinal net water flux at three different levels of the small intestine (duodenum, jejunum, ileum), by a technique of in situ closed loops in anaesthetised rats. VIP-stimulated net water flux was efficiently inhibited by both peptides at all three intestinal levels studied; PYY (ID50 about 30 pmol/kg.h) was 3 to 18 fold more potent than NPY. PGE2-stimulated net water flux was also efficiently inhibited in the jejunum and ileum; PYY (ID50 about 10 pmol/kg.h) was 30 to 90 fold more potent than NPY. A 30% inhibition of PGE2-stimulated net water flux could only be achieved in the duodenum with the largest dose of either peptide used in this study. PYY and NPY display potent inhibitory effects of stimulated net water flux at the three studied levels of the small intestine, except in the PGE2-stimulated duodenum. The PYY ID50s measured suggest that PYY may have ...
Journal of Medicinal Food, 2015