Safaa Tohamy - Academia.edu (original) (raw)

Papers by Safaa Tohamy

Research paper thumbnail of Histopathological Analysis and Comparison between Experimentally Fabricated Hydroxyapatite from Nile Tilapia Bone and Mineral Trioxide Aggregate as A Direct Pulp Capping Agents on Dog Pulp (In Vivo Study)

Open Access Macedonian Journal of Medical Sciences, Aug 14, 2022

AIM: The aim of this study was to study response of exposed dental dog pulp to the HP from Nile T... more AIM: The aim of this study was to study response of exposed dental dog pulp to the HP from Nile Tilapia bone and MTA on induction of reparative dentin formation and to inflammatory response in pulp tissue. MATERIALS AND METHODS: Four male mongrel dogs 1 year old with total of 20 teeth from each dog were selected and divided into two groups that each group has 40 teeth according to the material used as pulp capping agents. The maxillary teeth and the mandibular teeth for each dog were capped by both materials alternatively; the cavities were then sealed by glass-ionomer cement. Each group divided in two groups according to 2-time intervals (4 weeks and 8 weeks). Animals were sacrificed and teeth were collected for histopathological analysis. RESULTS: Specimens capped by fish bone powder after 4 weeks, which showed non-significantly higher of inflammatory cell scores than that capped by MTA. It showed thin (score 1) to moderate (score 2) thickness of dentine bridge formation, mostly a-tubular dentin, in all specimens. After 8 weeks, significant decrease in inflammatory cell infiltration scores for both groups were found, but it was significant in case of fish bone powder. Furthermore, dentine bridge became more significant for both materials with slight histological change in the group capped by fish bone powder. CONCLUSIONS: HA from Nile Tilapia bone could be considered as a direct pulp capping material. Furthermore, more studies needed on the fabrication of this material to give better result in shape and the pattern of reparative dentine formed.

Research paper thumbnail of Flow Cytometry Analysis of DNA Ploidy and S-Phase Fraction in Salivary Gland Tumors of Egyptian Patients

Aim: The aim of this study is to investigate the DNA ploidy and S-Phase Fraction (SPF) of some Sa... more Aim: The aim of this study is to investigate the DNA ploidy and S-Phase Fraction (SPF) of some Salivary Gland Tumors (SGTs) in Egyptian patients and to investigate the correlation between these two biological parameters and the presumptive behavior of these neoplasms. Methods: Flow cytometric analysis of DNA pliody and S-Phase Fraction (SPF) was done in 50 fresh tumor tissue sections of SGTs which diagnosed as 15 benign and 35 malignant tumors. Results: 93.3% of the benign SGTs tumors were diploid, while only 34% of malignant tumors were diploid and 66% were aneuploid. The malignant SGTs had higher SPF than the benign tumors but with no significant difference. There was no significant correlation of ploidy status or SPF with the tumor grades of mucoepidermoid carcinomas. Conclusion: DNA aneuploidy may be a key indicator for tumors activity and malignancy in SGTs, while the SPF has a minor role in the evaluation of SGTs activity.

Research paper thumbnail of Possible Effect of Pyrogallol on Tongue Squamous Cell Carcinoma SCC-25 Cells

Minia Journal of Medical Research

Background: Oral squamous cell carcinoma (OSCC) is one of the most prevalent oral cancers, despit... more Background: Oral squamous cell carcinoma (OSCC) is one of the most prevalent oral cancers, despite rapid improvement in chemotherapy, radiotherapy, and targeted gene therapy used in conjunction with mainstay surgery, the OSCC prognosis remains low. Previous studies have demonstrated anti-proliferative activity of polyphenols in cancer cells, Pyrogallol (PG) one of natural polyphenols, with cytotoxicity and apoptogenic effects on neoplastic and transformed cells, but not in normal cells. Objective: This study aims to investigate the effect of PG on tongue squamous cell carcinoma SCC-25 cells and evaluate possible apoptotic pathways on SCC-25 cells. Methods: Cytotoxicity was measured by MTT assay. Mode of cell death was determined using Image morphometric analysis and Estimation of caspase-3 by Real Time PCR. Results: MTT results showed that SCC-25 cells were sensitive to PG with an IC 50 value of 19.17 ug/ml. Image morphometric analysis showed a decrease in the mean values of Nuclear Area Factor (NAF) of SCC-25 cells treated with Different concentrations of PG when compared to control cells. In addition, PG induced apoptosis by simultaneous up-regulation of capase-3.Conclusions: Different concentrations of PG have a cytotoxic effect on SCC-25 cells. PG was also found to be an effective anti-carcinogenic agent with less toxicity.

Research paper thumbnail of Combined Effect of Doxorubicin and Pyrogallol on Tongue Squamous Cell Carcinoma SCC-25 Cells, an in vitro Study

 Background: Oral squamous cell carcinoma (OSCC) is the pathological type of oral cancer. Chemot... more  Background: Oral squamous cell carcinoma (OSCC) is the pathological type of oral cancer. Chemotherapy has become increasingly effective in OSCC therapy. Of these agents, Doxorubicin (Dox) is the most efficient cytotoxic agent but is prevented by cardiac toxicity. Several experimental results demonstrated the use of a combination of low-toxic secondary metabolites, such as polyphenols, to reduce the required dose of cytotoxic agents in cancer treatment.  Objective: The study aims to analyze the influence of Dox alone and its combination with pyrogallol (PG), a phenolic extract on the tongue SCC-25-cell, and to evaluate possible apoptotic pathways in the SCC-25 cells.  Methods: Image morphometric analysis for the estimation of nuclear area factor (NAF) and Evaluation of caspase-3 fold change by Real-Time PCR.  Results: The data recorded showed a significant decrease in the mean values of NAF of SCC-25 cells treated with different concentrations of Dox and its combination with PG....

Research paper thumbnail of Evaluation of Midkine as an Angiogentic and Prognostic Marker in Oral Squamous Cell Carcinoma

Egyptian dental journal

Midkine is a heparin-binding growth factor that promotes the proliferation, survival, migration a... more Midkine is a heparin-binding growth factor that promotes the proliferation, survival, migration and differentiation of various target cells. Midkine plays an important role in tumorigenesis and tumor progression, and is overexpressed in many human malignant tumors. The aims of this study were to evaluate the expression of von Willebrand Factor (vWF) and Midkine (MK) factor in oral squamous cell carcinoma (OSCC).And to correlate the expression of both (vWF) and (MK) with some clinicopathological data and lymph node metastasis. Immunohistochemical showed that MK protein expression was significantly higher in specimens of carcinomas with lymph node metastasis. Furthermore, vWF expression tended to be higher in cases that exhibited high expression of MK. These results suggest that MK may play important role in tumor's progression and angiogenesis.

Research paper thumbnail of Histopathological Analysis and Comparison between Experimentally Fabricated Hydroxyapatite from Nile Tilapia Bone and Mineral Trioxide Aggregate as A Direct Pulp Capping Agents on Dog Pulp (In Vivo Study)

Open Access Macedonian Journal of Medical Sciences, Aug 14, 2022

AIM: The aim of this study was to study response of exposed dental dog pulp to the HP from Nile T... more AIM: The aim of this study was to study response of exposed dental dog pulp to the HP from Nile Tilapia bone and MTA on induction of reparative dentin formation and to inflammatory response in pulp tissue. MATERIALS AND METHODS: Four male mongrel dogs 1 year old with total of 20 teeth from each dog were selected and divided into two groups that each group has 40 teeth according to the material used as pulp capping agents. The maxillary teeth and the mandibular teeth for each dog were capped by both materials alternatively; the cavities were then sealed by glass-ionomer cement. Each group divided in two groups according to 2-time intervals (4 weeks and 8 weeks). Animals were sacrificed and teeth were collected for histopathological analysis. RESULTS: Specimens capped by fish bone powder after 4 weeks, which showed non-significantly higher of inflammatory cell scores than that capped by MTA. It showed thin (score 1) to moderate (score 2) thickness of dentine bridge formation, mostly a-tubular dentin, in all specimens. After 8 weeks, significant decrease in inflammatory cell infiltration scores for both groups were found, but it was significant in case of fish bone powder. Furthermore, dentine bridge became more significant for both materials with slight histological change in the group capped by fish bone powder. CONCLUSIONS: HA from Nile Tilapia bone could be considered as a direct pulp capping material. Furthermore, more studies needed on the fabrication of this material to give better result in shape and the pattern of reparative dentine formed.

Research paper thumbnail of Flow Cytometry Analysis of DNA Ploidy and S-Phase Fraction in Salivary Gland Tumors of Egyptian Patients

Aim: The aim of this study is to investigate the DNA ploidy and S-Phase Fraction (SPF) of some Sa... more Aim: The aim of this study is to investigate the DNA ploidy and S-Phase Fraction (SPF) of some Salivary Gland Tumors (SGTs) in Egyptian patients and to investigate the correlation between these two biological parameters and the presumptive behavior of these neoplasms. Methods: Flow cytometric analysis of DNA pliody and S-Phase Fraction (SPF) was done in 50 fresh tumor tissue sections of SGTs which diagnosed as 15 benign and 35 malignant tumors. Results: 93.3% of the benign SGTs tumors were diploid, while only 34% of malignant tumors were diploid and 66% were aneuploid. The malignant SGTs had higher SPF than the benign tumors but with no significant difference. There was no significant correlation of ploidy status or SPF with the tumor grades of mucoepidermoid carcinomas. Conclusion: DNA aneuploidy may be a key indicator for tumors activity and malignancy in SGTs, while the SPF has a minor role in the evaluation of SGTs activity.

Research paper thumbnail of Possible Effect of Pyrogallol on Tongue Squamous Cell Carcinoma SCC-25 Cells

Minia Journal of Medical Research

Background: Oral squamous cell carcinoma (OSCC) is one of the most prevalent oral cancers, despit... more Background: Oral squamous cell carcinoma (OSCC) is one of the most prevalent oral cancers, despite rapid improvement in chemotherapy, radiotherapy, and targeted gene therapy used in conjunction with mainstay surgery, the OSCC prognosis remains low. Previous studies have demonstrated anti-proliferative activity of polyphenols in cancer cells, Pyrogallol (PG) one of natural polyphenols, with cytotoxicity and apoptogenic effects on neoplastic and transformed cells, but not in normal cells. Objective: This study aims to investigate the effect of PG on tongue squamous cell carcinoma SCC-25 cells and evaluate possible apoptotic pathways on SCC-25 cells. Methods: Cytotoxicity was measured by MTT assay. Mode of cell death was determined using Image morphometric analysis and Estimation of caspase-3 by Real Time PCR. Results: MTT results showed that SCC-25 cells were sensitive to PG with an IC 50 value of 19.17 ug/ml. Image morphometric analysis showed a decrease in the mean values of Nuclear Area Factor (NAF) of SCC-25 cells treated with Different concentrations of PG when compared to control cells. In addition, PG induced apoptosis by simultaneous up-regulation of capase-3.Conclusions: Different concentrations of PG have a cytotoxic effect on SCC-25 cells. PG was also found to be an effective anti-carcinogenic agent with less toxicity.

Research paper thumbnail of Combined Effect of Doxorubicin and Pyrogallol on Tongue Squamous Cell Carcinoma SCC-25 Cells, an in vitro Study

 Background: Oral squamous cell carcinoma (OSCC) is the pathological type of oral cancer. Chemot... more  Background: Oral squamous cell carcinoma (OSCC) is the pathological type of oral cancer. Chemotherapy has become increasingly effective in OSCC therapy. Of these agents, Doxorubicin (Dox) is the most efficient cytotoxic agent but is prevented by cardiac toxicity. Several experimental results demonstrated the use of a combination of low-toxic secondary metabolites, such as polyphenols, to reduce the required dose of cytotoxic agents in cancer treatment.  Objective: The study aims to analyze the influence of Dox alone and its combination with pyrogallol (PG), a phenolic extract on the tongue SCC-25-cell, and to evaluate possible apoptotic pathways in the SCC-25 cells.  Methods: Image morphometric analysis for the estimation of nuclear area factor (NAF) and Evaluation of caspase-3 fold change by Real-Time PCR.  Results: The data recorded showed a significant decrease in the mean values of NAF of SCC-25 cells treated with different concentrations of Dox and its combination with PG....

Research paper thumbnail of Evaluation of Midkine as an Angiogentic and Prognostic Marker in Oral Squamous Cell Carcinoma

Egyptian dental journal

Midkine is a heparin-binding growth factor that promotes the proliferation, survival, migration a... more Midkine is a heparin-binding growth factor that promotes the proliferation, survival, migration and differentiation of various target cells. Midkine plays an important role in tumorigenesis and tumor progression, and is overexpressed in many human malignant tumors. The aims of this study were to evaluate the expression of von Willebrand Factor (vWF) and Midkine (MK) factor in oral squamous cell carcinoma (OSCC).And to correlate the expression of both (vWF) and (MK) with some clinicopathological data and lymph node metastasis. Immunohistochemical showed that MK protein expression was significantly higher in specimens of carcinomas with lymph node metastasis. Furthermore, vWF expression tended to be higher in cases that exhibited high expression of MK. These results suggest that MK may play important role in tumor's progression and angiogenesis.