Salwa Kawashty - Academia.edu (original) (raw)

Papers by Salwa Kawashty

Zeitschrift für Naturforschung B, Apr 1, 2009

The cytotoxicity of the methanol extract of Origanum syriacum against human cerivical adenocarcin... more The cytotoxicity of the methanol extract of Origanum syriacum against human cerivical adenocarcinoma, Hela cells, was evaluated by the MTT assay. From the crude extract, a new flavone glycoside, acacetin-7-O-[2-O-α-L-rhamnopyranosyl-6-O-β-D-glucopyranosyl]-β-D-glucopyranoside (1), together with the ten known flavonoids luteolin (2), apigenin (3), luteolin-6-C-glucoside (4), luteolin-3-methylether-6-C-glucoside (5), luteolin-7,4-dimethyether-6-C-glucoside (6), apigenin-7methylether-6-C-glucoside (7), apigenin-7-O-glucoside (8), diosmetin-7-O-glucoside (9), acacetin-7-O-glucoside (10) and acacetin-7-O-rutinoside (11), was isolated from the methanol extract of the aerial parts of Origanum syriacum. The structure elucidation of the isolated compounds was performed by spectroscopic and chromatographic methods.

Biochemical Systematics and Ecology, 1990

Nineteen Euphorbia species were studied for their flavonoid patterns. Flavonoids identified were ... more Nineteen Euphorbia species were studied for their flavonoid patterns. Flavonoids identified were kaempferol, quercetin, apigenin and luteolin. Major glycosylation patterns are discussed.

Metabolites

Matthiola longipetala subsp. livida is an annual herb in Brassicaceae that has received little at... more Matthiola longipetala subsp. livida is an annual herb in Brassicaceae that has received little attention despite the family’s high reputation for health benefits, particularly cancer prevention. In this study, UPLC-HRMS-MS analysis was used for mapping the chemical constituents of different plant parts (i.e., flowers, leaves, and roots). Also, spectral similarity networks via the Global Natural Products Social Molecular Networking (GNPS) were employed to visualize their chemical differences and similarities. Additionally, the cytotoxic activity on HCT-116, HeLa, and HepG2 cell lines was evaluated. Throughout the current analysis, 154 compounds were annotated, with the prevalence of phenolic acids, glucosinolates, flavonol glucosides, lipids, peptides, and others. Predictably, secondary metabolites (phenolic acids, flavonoids, and glucosinolates) were predominant in flowers and leaves, while the roots were characterized by primary metabolites (peptides and fatty acids). Four diacetyl...

Botany ABSTRACT The present work focused on isolation, visualizing endophytic bacteria in its nat... more Botany ABSTRACT The present work focused on isolation, visualizing endophytic bacteria in its natural niche, inside plant tissue,and identification of cultivableendophytic bacteria that colonize aerial parts ofCapparissinaica, as anindigenous plant to Sinai-Egypt. C.sinaicais a medicinal plant was used in the treatment of several human diseasesin traditional medicines.The morphological, biochemical and 16Sr RNA gene sequence analysisrevealed thatBacillus species were the most dominantendophytic bacteria.Mixed microbial culture system of isolated endophyticbacterial species wasused for production of secondary metabolites.Paper Chromatography and HPLC analysis showed that bacterial extract producedquercetin andquercetin 3-O-glucopyranoside which were detected in plant extract. Hence, it was concluded that endophytic bacteria produced flavonoid compoundsin vitro similar to that originally produced by the plant itselfconfirming theidea of considering endophytes as the potential sources of novel natural products.

Journal of Natural Remedies, 2004

Objective: In this study, we aimed to identify the minor flavonoids of the areal parts of Vicia s... more Objective: In this study, we aimed to identify the minor flavonoids of the areal parts of Vicia sativa , a biologically active plant growing in El-Hassana, North Sinai, Egypt, and to monitor the antioxidant, anti-inflammatory, and antinociceptive activities of the plant extract and its purified flavonoids. Methods: antioxiant activity of V. sativa was assessed by four different assays including DPPH assay, quenching of ROO radicals, respiratory burst in rat neutrophils, and lipid peroxidation. On the other hand, antiinflammatory and antinociceptive effects of V. sativa and its flavonoids were assessed by carrageenaninduced inflammation in mice, acetic acid-induced writhing and formalin-licking test. Results: Nine flavonoids were isolated from the ethanol extract of the aerial parts of V. sativa and identified as active components: apigenin 4'-O-β-D-glucopyranoside [1], apigenin 6,8-di-C-β-D-glucopyranoside [2], apigenin 6-C-α-L-arabinopyranoside-8-C-β-D-glucopyranoside [3], kaem...

Arabian Journal of Chemistry, 2017

Four selected species of family Polygonaceae Juss. viz. Persicaria salicifolia (Brouss. ex Willd.... more Four selected species of family Polygonaceae Juss. viz. Persicaria salicifolia (Brouss. ex Willd.) Assenov, Persicaria senegalensis (Meisn.) Soja´k, Polygonum bellardii All. and Polygonum equisetiforme Sm. were subjected to botanical, chemical and numerical studies. The botanical part deals with macro-and micromorphological characters of the whole plant. The chemical part deals with extraction and identification of 17 compounds including flavones, flavonols, flavone C-glycosides and phenolic acids. The botanical and chemical results of the four selected species were subjected to a numerical analysis.

Chemistry of Natural Compounds, 2009

Brassicaceae is one of the largest angiosperm families, comprising approximately 338 genera and m... more Brassicaceae is one of the largest angiosperm families, comprising approximately 338 genera and more than 3709 species distributed worldwide [1]. It includes many economically important vegetable salad plants and crop species. Some of Cruciferae are grown domestically in gardens as ornamentals. The seeds of Cruciferae are largely used as condiment and fertilizer [2]. The plants of this family are used in the treatment of many diseases because of their anticancer, antibacterial, antifungal, antirheumatic, and antidiabetic properties [3]. The genus Farsetia is represented by three species that grow in Egypt. The most common one is Farsetia aegyptia Turra. [4, 5]. The plant is known to be used by native Bedouins as antidiabetic and antispasmodic. Moreover, it is used for the relief of rheumatic pains and taken internally as cooling medicine after pounding [3]. The powdered aerial parts of Farsetia aegyptia Turra. was defatted with petroleum ether (40-60°C) and extracted three times at room temperature with 70% methanol-water. The methanolic extract was fractionated and chromatographed [6, 7]. A new naturally occurring flavonol triglycoside [8], kaempferol-3-O-(2′′-α-L-arabinopyranosyl)-α-L-rhamnopyranoside-7-O-α-L-rhamnopyranoside (1), was isolated, together with eight known flavonoids: kaempferol-3-O-(2′′-β-D-glucopyranosyl)α-L-rhamnopyranoside-7-O-α-L-rhamnopyranoside (2), kaempferol-3,7-di-O-α-L-rhamnopyranoside (3), kaempferol (4), isorhamnetin-3-O-β-L-arabinopyranoside-7-O-(2′′′-β-D-glucopyranosyl)-α-L-rhamnopyranoside (5), isorhamnetin-3-O-α-Lrhamnopyranosyl-7-O-β-D-glucopyranoside (6), isorhamnetin (7), apigenin-7-O-β-D-glucopyranoside (8), and apigenin (9). Compound 1 was isolated as a pale yellow amorphous powder. The color reaction on paper chromatography with ammonia vapor changed from brown to yellow, indicating that the compound has a flavonoid chromophore [6]. UV spectral data with diagnostic shift reagents suggested the presence of a 3,7-disubstituted flavonol glycoside with free hydroxyl groups at the 5 and 4′ positions [6, 7]. Two intermediate spots were detected upon mild acid hydrolysis with 0.1 N HCl, before yielding the aglycone. Complete acid hydrolysis (2 N HCl, 1 h, 100°C) yielded rhamnose and arabinose (Co-PC) and kaempferol (3,5,7,4′-tetrahydroxyflavone) (Co-PC, UV, EIMS, and 1 H NMR). The negative-ion FABMS showed a molecular ion peak [M-H]at m/z 709, corresponding to C 32 H 38 O 18. The 1 H NMR spectrum showed two pairs of doublets at δ 7.8 (J = 9.0 Hz) and δ 6.95 (J = 9.0 Hz) assigned to H-2′,6′ and H-3′,5′, respectively. The two meta coupled doublets at δ 6.7 (J = 2.0 Hz) and δ 6.4 (J = 2.0 Hz) are assigned to H-8 and H-6, respectively. This downfield chemical shift confirmed that C-7 was substituted in ring A [6]. The 1 H NMR spectrum also revealed three distinct anomeric proton resonances at δ 5.54 (J = 2 Hz), δ 5.38 (J =2 Hz), and δ 4.2 (J = 6.9 Hz), attributed to H-1′′′ of the α-rhamnopyranose unit at position 7, H-1′′ of the α-rhamnopyranose at position 3, and H-1′′′′ of the α-arabinopyranose unit, respectively [9]. These data also confirmed the presence of two signals at δ 0.9 and δ 1.1. The chemically shifted signal at δ 0.

Chemistry of Natural Compounds, 2007

Abstract A new kaempferol 3-O-(2-β-D-glucopyranosyl)-α-L-rhamnopyranoside-7-O-α-L-rhamnopyranosid... more Abstract A new kaempferol 3-O-(2-β-D-glucopyranosyl)-α-L-rhamnopyranoside-7-O-α-L-rhamnopyranoside and eight known flavonoid compounds were isolated and identified. A biological study includes determination of LD 50, anti-inflammatory effect, analgesic effect, ...

Biochemical Systematics and Ecology, 2010

The flavonoids of nine selected species belonging to different tribes of family Brassicaceae (Cru... more The flavonoids of nine selected species belonging to different tribes of family Brassicaceae (Cruciferae) native to Egypt were surveyed, viz. Rorippa palustris, Coronopus squamatus, Eremobium aegyptiacum, Moricandia nitens, Brassica tournefortii, Farsetia aegyptia, Matthiola livida, Anastatica hierochuntica and Sisymbrium irio. Thirty-eight compounds were isolated and identified, which included six flavonol aglycones, 24 flavonol glycosides including 14 flavonol 3,7-diglycosides, one flavone aglycone, three flavone O-glycosides, two glycoflavones and two dihydroflavonoids. A numerical analysis based on a combination of 97 morphological, anatomical and chemical characters revealed two series, two subseries, two clusters and two groups. The interrelationships between the studied species are discussed.

Biochemical Systematics and Ecology, 1994

Flavonoid glycosides of 18 Plantago species belonging to six sections were investigated. Twentyfi... more Flavonoid glycosides of 18 Plantago species belonging to six sections were investigated. Twentyfive glycosides were found to belong to the aglycones apigenin, luteolin, chrysoeriol, tricin and the less common hispidulin, nepetin and 5,6,7,3'-tetrahydroxy4'-methoxyflavone. Only one flavonol glycoside (isorhamnetin 3-rutinoside) was detected in three species belonging to sect. Leucopsyllium. Chemosystematic relationships are discussed.

Biochemical Systematics and Ecology, 2011

International Journal of Secondary Metabolite

Diabetes is a chronic metabolic disease that creates high blood sugar level. Therefore, diabetes ... more Diabetes is a chronic metabolic disease that creates high blood sugar level. Therefore, diabetes awareness is necessary to prevent diabetes by reducing sugar intake and using low-calorie alternative sweeteners instead. Stevia rebaudiana is a medicinal plant species belonging to the Compositae family. It is a sweet herb that contains diterpene glycosides, which are directly responsible for the sweet taste, but they have no caloric value. Since ancient times, there have been several reports on the use of S. rebaudiana as an alternative sweetener and extended research has been conducted on its phytochemicals and biological activities. The plant contains a good number of phytochemicals with significant biological activities, namely polyphenolic derivatives, diterpenes glycosides, alkaloids, glycosides, tannins, chlorophylls, carotenoids, etc. For industrial use, those phytochemicals could be extracted from the selected plant and used for the preparation of nutraceuticals and food additi...

Natural Product Research, 2013

Two new flavonoid compounds were isolated from Astragalus bombycinus Boiss. and identified as que... more Two new flavonoid compounds were isolated from Astragalus bombycinus Boiss. and identified as quercetin-3,7-di-O-β-glucopyranoside 4'-O-α-rhamnopyranoside and 5,2',4'-trihydroxy-flavone-8-C-α-arabinopyranoside-7-O-β-glucopyranoside. In addition, apigenin, apigenin-7-O-β-glucopyranoside, apigenin 7-O-gentobioside, luteolin, luteolin-7-O-β-glucopyranoside, quercetin-3,7-di-O-β-glucopyranoside, quercetin-3-O-β-glucopyranoside-7-O-α-rhamnopyranoside and daidzein were also isolated and identified. The structure elucidation of the isolated compounds was performed by chromatographic, chemical and spectroscopic methods. Antioxidant and cytotoxic activities were also determined for the four consecutive extracts of the plant.

The present study aims to detect the flavonoid and phenolic constituents of six selected species ... more The present study aims to detect the flavonoid and phenolic constituents of six selected species belonging to different families represented in Mahd adh Dhahab region as well as screen their antioxidant and cytotoxic activities. These are Senna alexandrina Mill. and Vachellia nilotica (L.) P.J.H.Hurter & Mabb. (Fabaceae), Vitex trifolia L. (Lamiaceae), Moltkiopsis ciliata (Forssk.) I. M. Johnston (Boraginaceae), Salicornia europaea L. (Chenopodiaceae) and Acanthus spinosus L. (Acanthaceae).The phenolic and flavonoid profiles were carried out using HPLC technique. In addition, in vivo antioxidant activity was performed using DPPH assay while the cytotoxicity was summarized by MTT test. Thirty three compounds were detected using HPLC technique and comparison with standards. They were detected as 14 phenolic acids and 19 flavonoids. The flavonoids were represented as five C-glycosyl flavones, five flavones, six flavonols and three flavanones. The DPPH assay showed moderate activities w...

Objective : Studies were carried out to evaluate the chemosystematic relationships of two Mentha ... more Objective : Studies were carried out to evaluate the chemosystematic relationships of two Mentha species (M. microphylla C. Koch and M. viridis L.). The biological activity of the extracts of both species was recorded. Methods : The isolation and identification of flavonoids were carried out using paper and column chromatography, UV, MS and NMR spectral analysis. Antimicrobial and cytotxic activities were studied using agar diffusion and mortality test, respectively. Results : Six flavonoidal compounds were isolated and identified from the aqueous methanolic extract of the aerial parts of the two Mentha species. Both ethanol fractions of the species showed a moderate activity against Escherichia coli , Gram negative bacteria while the other fractions and pure compounds were inactive. Conclusion: The phytochemical investigation of the two Mentha species showed a clear difference in their flavonoid profile. The distribution of the identified flavonoid glycosides in the two taxa invest...

Biochemical Systematics and Ecology, 2000

Natural Product Research, 2008

and-conditions-of-access.pdf This article may be used for research, teaching and private study pu... more and-conditions-of-access.pdf This article may be used for research, teaching and private study purposes. Any substantial or systematic reproduction, redistribution , reselling , loan or sub-licensing, systematic supply or distribution in any form to anyone is expressly forbidden. The publisher does not give any warranty express or implied or make any representation that the contents will be complete or accurate or up to date. The accuracy of any instructions, formulae and drug doses should be independently verified with primary sources. The publisher shall not be liable for any loss, actions, claims, proceedings, demand or costs or damages whatsoever or howsoever caused arising directly or indirectly in connection with or arising out of the use of this material.

Journal of Applied Pharmaceutical Science

Mentha longifolia (L.) Huds. and Mentha pulegium L. are growing wild in Egyptian flora, they have... more Mentha longifolia (L.) Huds. and Mentha pulegium L. are growing wild in Egyptian flora, they have many biological activities and commonly used as traditional herbal medicines. The aqueous methanolic extracts of the two Egyptian species were subjected to a comparative study for the first time using the LC-ESI-MS fingerprint. 43 compounds belonging to different chemical classes (flavone, flavonol, flavanone, C-glycosylflavone, phenolic acids and their glycosides) have been identified. The identified compounds can be used as chemosystematic markers and further our knowledge of the infraspecific relationship between the studied species. Further isolation and in vitro testing of metabolites will provide more evidence for the possible uses of Mentha sp.

Zeitschrift für Naturforschung B, Apr 1, 2009

The cytotoxicity of the methanol extract of Origanum syriacum against human cerivical adenocarcin... more The cytotoxicity of the methanol extract of Origanum syriacum against human cerivical adenocarcinoma, Hela cells, was evaluated by the MTT assay. From the crude extract, a new flavone glycoside, acacetin-7-O-[2-O-α-L-rhamnopyranosyl-6-O-β-D-glucopyranosyl]-β-D-glucopyranoside (1), together with the ten known flavonoids luteolin (2), apigenin (3), luteolin-6-C-glucoside (4), luteolin-3-methylether-6-C-glucoside (5), luteolin-7,4-dimethyether-6-C-glucoside (6), apigenin-7methylether-6-C-glucoside (7), apigenin-7-O-glucoside (8), diosmetin-7-O-glucoside (9), acacetin-7-O-glucoside (10) and acacetin-7-O-rutinoside (11), was isolated from the methanol extract of the aerial parts of Origanum syriacum. The structure elucidation of the isolated compounds was performed by spectroscopic and chromatographic methods.

Biochemical Systematics and Ecology, 1990

Nineteen Euphorbia species were studied for their flavonoid patterns. Flavonoids identified were ... more Nineteen Euphorbia species were studied for their flavonoid patterns. Flavonoids identified were kaempferol, quercetin, apigenin and luteolin. Major glycosylation patterns are discussed.

Metabolites

Matthiola longipetala subsp. livida is an annual herb in Brassicaceae that has received little at... more Matthiola longipetala subsp. livida is an annual herb in Brassicaceae that has received little attention despite the family’s high reputation for health benefits, particularly cancer prevention. In this study, UPLC-HRMS-MS analysis was used for mapping the chemical constituents of different plant parts (i.e., flowers, leaves, and roots). Also, spectral similarity networks via the Global Natural Products Social Molecular Networking (GNPS) were employed to visualize their chemical differences and similarities. Additionally, the cytotoxic activity on HCT-116, HeLa, and HepG2 cell lines was evaluated. Throughout the current analysis, 154 compounds were annotated, with the prevalence of phenolic acids, glucosinolates, flavonol glucosides, lipids, peptides, and others. Predictably, secondary metabolites (phenolic acids, flavonoids, and glucosinolates) were predominant in flowers and leaves, while the roots were characterized by primary metabolites (peptides and fatty acids). Four diacetyl...

Botany ABSTRACT The present work focused on isolation, visualizing endophytic bacteria in its nat... more Botany ABSTRACT The present work focused on isolation, visualizing endophytic bacteria in its natural niche, inside plant tissue,and identification of cultivableendophytic bacteria that colonize aerial parts ofCapparissinaica, as anindigenous plant to Sinai-Egypt. C.sinaicais a medicinal plant was used in the treatment of several human diseasesin traditional medicines.The morphological, biochemical and 16Sr RNA gene sequence analysisrevealed thatBacillus species were the most dominantendophytic bacteria.Mixed microbial culture system of isolated endophyticbacterial species wasused for production of secondary metabolites.Paper Chromatography and HPLC analysis showed that bacterial extract producedquercetin andquercetin 3-O-glucopyranoside which were detected in plant extract. Hence, it was concluded that endophytic bacteria produced flavonoid compoundsin vitro similar to that originally produced by the plant itselfconfirming theidea of considering endophytes as the potential sources of novel natural products.

Journal of Natural Remedies, 2004

Objective: In this study, we aimed to identify the minor flavonoids of the areal parts of Vicia s... more Objective: In this study, we aimed to identify the minor flavonoids of the areal parts of Vicia sativa , a biologically active plant growing in El-Hassana, North Sinai, Egypt, and to monitor the antioxidant, anti-inflammatory, and antinociceptive activities of the plant extract and its purified flavonoids. Methods: antioxiant activity of V. sativa was assessed by four different assays including DPPH assay, quenching of ROO radicals, respiratory burst in rat neutrophils, and lipid peroxidation. On the other hand, antiinflammatory and antinociceptive effects of V. sativa and its flavonoids were assessed by carrageenaninduced inflammation in mice, acetic acid-induced writhing and formalin-licking test. Results: Nine flavonoids were isolated from the ethanol extract of the aerial parts of V. sativa and identified as active components: apigenin 4'-O-β-D-glucopyranoside [1], apigenin 6,8-di-C-β-D-glucopyranoside [2], apigenin 6-C-α-L-arabinopyranoside-8-C-β-D-glucopyranoside [3], kaem...

Arabian Journal of Chemistry, 2017

Four selected species of family Polygonaceae Juss. viz. Persicaria salicifolia (Brouss. ex Willd.... more Four selected species of family Polygonaceae Juss. viz. Persicaria salicifolia (Brouss. ex Willd.) Assenov, Persicaria senegalensis (Meisn.) Soja´k, Polygonum bellardii All. and Polygonum equisetiforme Sm. were subjected to botanical, chemical and numerical studies. The botanical part deals with macro-and micromorphological characters of the whole plant. The chemical part deals with extraction and identification of 17 compounds including flavones, flavonols, flavone C-glycosides and phenolic acids. The botanical and chemical results of the four selected species were subjected to a numerical analysis.

Chemistry of Natural Compounds, 2009

Brassicaceae is one of the largest angiosperm families, comprising approximately 338 genera and m... more Brassicaceae is one of the largest angiosperm families, comprising approximately 338 genera and more than 3709 species distributed worldwide [1]. It includes many economically important vegetable salad plants and crop species. Some of Cruciferae are grown domestically in gardens as ornamentals. The seeds of Cruciferae are largely used as condiment and fertilizer [2]. The plants of this family are used in the treatment of many diseases because of their anticancer, antibacterial, antifungal, antirheumatic, and antidiabetic properties [3]. The genus Farsetia is represented by three species that grow in Egypt. The most common one is Farsetia aegyptia Turra. [4, 5]. The plant is known to be used by native Bedouins as antidiabetic and antispasmodic. Moreover, it is used for the relief of rheumatic pains and taken internally as cooling medicine after pounding [3]. The powdered aerial parts of Farsetia aegyptia Turra. was defatted with petroleum ether (40-60°C) and extracted three times at room temperature with 70% methanol-water. The methanolic extract was fractionated and chromatographed [6, 7]. A new naturally occurring flavonol triglycoside [8], kaempferol-3-O-(2′′-α-L-arabinopyranosyl)-α-L-rhamnopyranoside-7-O-α-L-rhamnopyranoside (1), was isolated, together with eight known flavonoids: kaempferol-3-O-(2′′-β-D-glucopyranosyl)α-L-rhamnopyranoside-7-O-α-L-rhamnopyranoside (2), kaempferol-3,7-di-O-α-L-rhamnopyranoside (3), kaempferol (4), isorhamnetin-3-O-β-L-arabinopyranoside-7-O-(2′′′-β-D-glucopyranosyl)-α-L-rhamnopyranoside (5), isorhamnetin-3-O-α-Lrhamnopyranosyl-7-O-β-D-glucopyranoside (6), isorhamnetin (7), apigenin-7-O-β-D-glucopyranoside (8), and apigenin (9). Compound 1 was isolated as a pale yellow amorphous powder. The color reaction on paper chromatography with ammonia vapor changed from brown to yellow, indicating that the compound has a flavonoid chromophore [6]. UV spectral data with diagnostic shift reagents suggested the presence of a 3,7-disubstituted flavonol glycoside with free hydroxyl groups at the 5 and 4′ positions [6, 7]. Two intermediate spots were detected upon mild acid hydrolysis with 0.1 N HCl, before yielding the aglycone. Complete acid hydrolysis (2 N HCl, 1 h, 100°C) yielded rhamnose and arabinose (Co-PC) and kaempferol (3,5,7,4′-tetrahydroxyflavone) (Co-PC, UV, EIMS, and 1 H NMR). The negative-ion FABMS showed a molecular ion peak [M-H]at m/z 709, corresponding to C 32 H 38 O 18. The 1 H NMR spectrum showed two pairs of doublets at δ 7.8 (J = 9.0 Hz) and δ 6.95 (J = 9.0 Hz) assigned to H-2′,6′ and H-3′,5′, respectively. The two meta coupled doublets at δ 6.7 (J = 2.0 Hz) and δ 6.4 (J = 2.0 Hz) are assigned to H-8 and H-6, respectively. This downfield chemical shift confirmed that C-7 was substituted in ring A [6]. The 1 H NMR spectrum also revealed three distinct anomeric proton resonances at δ 5.54 (J = 2 Hz), δ 5.38 (J =2 Hz), and δ 4.2 (J = 6.9 Hz), attributed to H-1′′′ of the α-rhamnopyranose unit at position 7, H-1′′ of the α-rhamnopyranose at position 3, and H-1′′′′ of the α-arabinopyranose unit, respectively [9]. These data also confirmed the presence of two signals at δ 0.9 and δ 1.1. The chemically shifted signal at δ 0.

Chemistry of Natural Compounds, 2007

Abstract A new kaempferol 3-O-(2-β-D-glucopyranosyl)-α-L-rhamnopyranoside-7-O-α-L-rhamnopyranosid... more Abstract A new kaempferol 3-O-(2-β-D-glucopyranosyl)-α-L-rhamnopyranoside-7-O-α-L-rhamnopyranoside and eight known flavonoid compounds were isolated and identified. A biological study includes determination of LD 50, anti-inflammatory effect, analgesic effect, ...

Biochemical Systematics and Ecology, 2010

The flavonoids of nine selected species belonging to different tribes of family Brassicaceae (Cru... more The flavonoids of nine selected species belonging to different tribes of family Brassicaceae (Cruciferae) native to Egypt were surveyed, viz. Rorippa palustris, Coronopus squamatus, Eremobium aegyptiacum, Moricandia nitens, Brassica tournefortii, Farsetia aegyptia, Matthiola livida, Anastatica hierochuntica and Sisymbrium irio. Thirty-eight compounds were isolated and identified, which included six flavonol aglycones, 24 flavonol glycosides including 14 flavonol 3,7-diglycosides, one flavone aglycone, three flavone O-glycosides, two glycoflavones and two dihydroflavonoids. A numerical analysis based on a combination of 97 morphological, anatomical and chemical characters revealed two series, two subseries, two clusters and two groups. The interrelationships between the studied species are discussed.

Biochemical Systematics and Ecology, 1994

Flavonoid glycosides of 18 Plantago species belonging to six sections were investigated. Twentyfi... more Flavonoid glycosides of 18 Plantago species belonging to six sections were investigated. Twentyfive glycosides were found to belong to the aglycones apigenin, luteolin, chrysoeriol, tricin and the less common hispidulin, nepetin and 5,6,7,3'-tetrahydroxy4'-methoxyflavone. Only one flavonol glycoside (isorhamnetin 3-rutinoside) was detected in three species belonging to sect. Leucopsyllium. Chemosystematic relationships are discussed.

Biochemical Systematics and Ecology, 2011

International Journal of Secondary Metabolite

Diabetes is a chronic metabolic disease that creates high blood sugar level. Therefore, diabetes ... more Diabetes is a chronic metabolic disease that creates high blood sugar level. Therefore, diabetes awareness is necessary to prevent diabetes by reducing sugar intake and using low-calorie alternative sweeteners instead. Stevia rebaudiana is a medicinal plant species belonging to the Compositae family. It is a sweet herb that contains diterpene glycosides, which are directly responsible for the sweet taste, but they have no caloric value. Since ancient times, there have been several reports on the use of S. rebaudiana as an alternative sweetener and extended research has been conducted on its phytochemicals and biological activities. The plant contains a good number of phytochemicals with significant biological activities, namely polyphenolic derivatives, diterpenes glycosides, alkaloids, glycosides, tannins, chlorophylls, carotenoids, etc. For industrial use, those phytochemicals could be extracted from the selected plant and used for the preparation of nutraceuticals and food additi...

Natural Product Research, 2013

Two new flavonoid compounds were isolated from Astragalus bombycinus Boiss. and identified as que... more Two new flavonoid compounds were isolated from Astragalus bombycinus Boiss. and identified as quercetin-3,7-di-O-β-glucopyranoside 4'-O-α-rhamnopyranoside and 5,2',4'-trihydroxy-flavone-8-C-α-arabinopyranoside-7-O-β-glucopyranoside. In addition, apigenin, apigenin-7-O-β-glucopyranoside, apigenin 7-O-gentobioside, luteolin, luteolin-7-O-β-glucopyranoside, quercetin-3,7-di-O-β-glucopyranoside, quercetin-3-O-β-glucopyranoside-7-O-α-rhamnopyranoside and daidzein were also isolated and identified. The structure elucidation of the isolated compounds was performed by chromatographic, chemical and spectroscopic methods. Antioxidant and cytotoxic activities were also determined for the four consecutive extracts of the plant.

The present study aims to detect the flavonoid and phenolic constituents of six selected species ... more The present study aims to detect the flavonoid and phenolic constituents of six selected species belonging to different families represented in Mahd adh Dhahab region as well as screen their antioxidant and cytotoxic activities. These are Senna alexandrina Mill. and Vachellia nilotica (L.) P.J.H.Hurter & Mabb. (Fabaceae), Vitex trifolia L. (Lamiaceae), Moltkiopsis ciliata (Forssk.) I. M. Johnston (Boraginaceae), Salicornia europaea L. (Chenopodiaceae) and Acanthus spinosus L. (Acanthaceae).The phenolic and flavonoid profiles were carried out using HPLC technique. In addition, in vivo antioxidant activity was performed using DPPH assay while the cytotoxicity was summarized by MTT test. Thirty three compounds were detected using HPLC technique and comparison with standards. They were detected as 14 phenolic acids and 19 flavonoids. The flavonoids were represented as five C-glycosyl flavones, five flavones, six flavonols and three flavanones. The DPPH assay showed moderate activities w...

Objective : Studies were carried out to evaluate the chemosystematic relationships of two Mentha ... more Objective : Studies were carried out to evaluate the chemosystematic relationships of two Mentha species (M. microphylla C. Koch and M. viridis L.). The biological activity of the extracts of both species was recorded. Methods : The isolation and identification of flavonoids were carried out using paper and column chromatography, UV, MS and NMR spectral analysis. Antimicrobial and cytotxic activities were studied using agar diffusion and mortality test, respectively. Results : Six flavonoidal compounds were isolated and identified from the aqueous methanolic extract of the aerial parts of the two Mentha species. Both ethanol fractions of the species showed a moderate activity against Escherichia coli , Gram negative bacteria while the other fractions and pure compounds were inactive. Conclusion: The phytochemical investigation of the two Mentha species showed a clear difference in their flavonoid profile. The distribution of the identified flavonoid glycosides in the two taxa invest...

Biochemical Systematics and Ecology, 2000

Natural Product Research, 2008

and-conditions-of-access.pdf This article may be used for research, teaching and private study pu... more and-conditions-of-access.pdf This article may be used for research, teaching and private study purposes. Any substantial or systematic reproduction, redistribution , reselling , loan or sub-licensing, systematic supply or distribution in any form to anyone is expressly forbidden. The publisher does not give any warranty express or implied or make any representation that the contents will be complete or accurate or up to date. The accuracy of any instructions, formulae and drug doses should be independently verified with primary sources. The publisher shall not be liable for any loss, actions, claims, proceedings, demand or costs or damages whatsoever or howsoever caused arising directly or indirectly in connection with or arising out of the use of this material.

Journal of Applied Pharmaceutical Science

Mentha longifolia (L.) Huds. and Mentha pulegium L. are growing wild in Egyptian flora, they have... more Mentha longifolia (L.) Huds. and Mentha pulegium L. are growing wild in Egyptian flora, they have many biological activities and commonly used as traditional herbal medicines. The aqueous methanolic extracts of the two Egyptian species were subjected to a comparative study for the first time using the LC-ESI-MS fingerprint. 43 compounds belonging to different chemical classes (flavone, flavonol, flavanone, C-glycosylflavone, phenolic acids and their glycosides) have been identified. The identified compounds can be used as chemosystematic markers and further our knowledge of the infraspecific relationship between the studied species. Further isolation and in vitro testing of metabolites will provide more evidence for the possible uses of Mentha sp.