Sam Smith - Academia.edu (original) (raw)
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Università Cattolica del Sacro Cuore (Catholic University of the Sacred Heart)
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Papers by Sam Smith
Faraday Discussions, 2004
EPR spectroscopic techniques have been developed for the measurement of oxygen and nitric oxide i... more EPR spectroscopic techniques have been developed for the measurement of oxygen and nitric oxide in vivo. Specifically, the methods for in vivo measurement of these molecules has been applied to the study of septic shock, utilising an experimental murine model developed in our laboratory. Oxygen was measured as pO 2 by the particlulate probes Gloxy and LiPc, which were surgically implanted at specific sites in tissues, and the soluble probe Trityl, which was administered intravenously. Nitric oxide was measured as the NO-Fe-(DETC) 2 complex after administration of Fe 2+ and DETC. LPS was seen to significantly decrease liver oxygen measured across the lobule and at the sinusoids by the Gloxy probe; there was a corresponding increase in nitric oxide both in the liver and systemically. The nitric oxide most likely originated from increased iNOS enzyme in the liver as demonstrated by Western blotting and the localisation of nitric oxide to the liver was confirmed with EPR imaging. LPS also caused a decrease in cerebral blood and tissue oxygenation, the rate of which was found to be dependent on the blood oxygenation. The development and applications of these in vivo EPR techniques for biomedical research and diagnostics is discussed.
EPR spectroscopic techniques have been developed for the measurement of oxygen and nitric oxide i... more EPR spectroscopic techniques have been developed for the measurement of oxygen and nitric oxide in vivo. Specifically, the methods for in vivo measurement of these molecules has been applied to the study of septic shock, utilising an experimental murine model developed in our laboratory. Oxygen was measured as pO 2 by the particlulate probes Gloxy and LiPc, which were surgically implanted at specific sites in tissues, and the soluble probe Trityl, which was administered intravenously. Nitric oxide was measured as the NO-Fe-(DETC) 2 complex after administration of Fe 2+ and DETC. LPS was seen to significantly decrease liver oxygen measured across the lobule and at the sinusoids by the Gloxy probe; there was a corresponding increase in nitric oxide both in the liver and systemically. The nitric oxide most likely originated from increased iNOS enzyme in the liver as demonstrated by Western blotting and the localisation of nitric oxide to the liver was confirmed with EPR imaging. LPS also caused a decrease in cerebral blood and tissue oxygenation, the rate of which was found to be dependent on the blood oxygenation. The development and applications of these in vivo EPR techniques for biomedical research and diagnostics is discussed.
Pediatric Research, 1985
Abstract Octeocalcin (OC) a calcium binding protein of bone matrix, appears to play a role in the... more Abstract Octeocalcin (OC) a calcium binding protein of bone matrix, appears to play a role in the regulation of mineral deposition and remodeling of bone. Elevated plasma levels of OC have been reported in some metabolic bone diseases with high bone turnover. We have ...
Pediatric Research, 1987
Pediatric Research. Wolters Kluwer Health Logo. All Issues. ...
Experimental Neurology, 1980
The plantaris muscle of the rat is innervated by fibers deriving from spinal nerves L4 and L5. Wh... more The plantaris muscle of the rat is innervated by fibers deriving from spinal nerves L4 and L5. When L4 is transected, the intact residual L5 fibers sprout intramuscular preterminal processes which reinnervate some of the denervated muscle fibers and restore their weight and ...
Faraday Discussions, 2004
EPR spectroscopic techniques have been developed for the measurement of oxygen and nitric oxide i... more EPR spectroscopic techniques have been developed for the measurement of oxygen and nitric oxide in vivo. Specifically, the methods for in vivo measurement of these molecules has been applied to the study of septic shock, utilising an experimental murine model developed in our laboratory. Oxygen was measured as pO 2 by the particlulate probes Gloxy and LiPc, which were surgically implanted at specific sites in tissues, and the soluble probe Trityl, which was administered intravenously. Nitric oxide was measured as the NO-Fe-(DETC) 2 complex after administration of Fe 2+ and DETC. LPS was seen to significantly decrease liver oxygen measured across the lobule and at the sinusoids by the Gloxy probe; there was a corresponding increase in nitric oxide both in the liver and systemically. The nitric oxide most likely originated from increased iNOS enzyme in the liver as demonstrated by Western blotting and the localisation of nitric oxide to the liver was confirmed with EPR imaging. LPS also caused a decrease in cerebral blood and tissue oxygenation, the rate of which was found to be dependent on the blood oxygenation. The development and applications of these in vivo EPR techniques for biomedical research and diagnostics is discussed.
EPR spectroscopic techniques have been developed for the measurement of oxygen and nitric oxide i... more EPR spectroscopic techniques have been developed for the measurement of oxygen and nitric oxide in vivo. Specifically, the methods for in vivo measurement of these molecules has been applied to the study of septic shock, utilising an experimental murine model developed in our laboratory. Oxygen was measured as pO 2 by the particlulate probes Gloxy and LiPc, which were surgically implanted at specific sites in tissues, and the soluble probe Trityl, which was administered intravenously. Nitric oxide was measured as the NO-Fe-(DETC) 2 complex after administration of Fe 2+ and DETC. LPS was seen to significantly decrease liver oxygen measured across the lobule and at the sinusoids by the Gloxy probe; there was a corresponding increase in nitric oxide both in the liver and systemically. The nitric oxide most likely originated from increased iNOS enzyme in the liver as demonstrated by Western blotting and the localisation of nitric oxide to the liver was confirmed with EPR imaging. LPS also caused a decrease in cerebral blood and tissue oxygenation, the rate of which was found to be dependent on the blood oxygenation. The development and applications of these in vivo EPR techniques for biomedical research and diagnostics is discussed.
Pediatric Research, 1985
Abstract Octeocalcin (OC) a calcium binding protein of bone matrix, appears to play a role in the... more Abstract Octeocalcin (OC) a calcium binding protein of bone matrix, appears to play a role in the regulation of mineral deposition and remodeling of bone. Elevated plasma levels of OC have been reported in some metabolic bone diseases with high bone turnover. We have ...
Pediatric Research, 1987
Pediatric Research. Wolters Kluwer Health Logo. All Issues. ...
Experimental Neurology, 1980
The plantaris muscle of the rat is innervated by fibers deriving from spinal nerves L4 and L5. Wh... more The plantaris muscle of the rat is innervated by fibers deriving from spinal nerves L4 and L5. When L4 is transected, the intact residual L5 fibers sprout intramuscular preterminal processes which reinnervate some of the denervated muscle fibers and restore their weight and ...