Samah S. Ibrahim - Academia.edu (original) (raw)
Papers by Samah S. Ibrahim
Nanoparticle compounds such as Moringa oleifera Lam. leaf powder, boiled white and brown eggshell... more Nanoparticle compounds such as Moringa oleifera Lam. leaf powder, boiled white and brown eggshells powder for chickens and kapritia spring water form Siwa Oasis in Egypt, these natural materials were evaluated for first time under laboratory conditions against the 4 th instar larvae of cotton leafworm Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae). The results investigated that new nanoparticles of all eggshells and M. oleifera powder and kapritia water affected on biological aspects of target pest such as, larval mortality were recorded that,10, 8, 6 and 4% for M. oleifera, kapritia water, brown and white eggshells, respectively, compared with control 1%, the intermediated shapes were recorded during this studied. Also adults moths emergency percentage of S.littoralis are decreased to 89 , 82, 73 and 65 % after treatment with white eggshells, brown eggshells , M. oleifera leaf powders and kapritia Siwa water, respectively, compared with control 93%. Subsequently, adults malformed increased after all treatments; fecundity (Eggs/ female), fertility and also were affected. These compounds considered as nanoparticle, these innovation technologies to enhance our country developmental strategies in order to achieved sustainable agriculture and integrated crop management by alternative insecticide, environmental protection form pollutant, increase natural enemies for insect and food safety for better human healthy for a new generation. Introduction The Egyptian cotton leafworm Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae) is the most dangerous pests in Egypt and Africa, causing significant economic loss of cotton, tomato, lettuce, strawberry and other vegetables crop in both greenhouses and open fields (Abd El-Razik and Mostafa, 2013 and EPPO, 2014). Moringa oleifera Lam. is medicinal plant with many pharmacological properties, leaves are a nutrient-dense food with high concentrations of protein, carbohydrates, fiber and a good source of several vitamins and minerals such as vitamins A, B, C, D, and E; folic acid; nicotinic acid; iron; calcium; zinc; potassium; magnesium and copper (Singh and Singh, 2019 and Walia et al., (2019). The chemical constituents of M.oleifera stems, leaves, flowers, pods and seeds are alkaloids, phenolicacids, gallic,
Dried fruit seeds; i.e. date pits powder of date palm trees (Phoenix dactylifera L.) swia variety... more Dried fruit seeds; i.e. date pits powder of date palm trees (Phoenix dactylifera L.) swia variety, wild custard apple seeds of (Annona senegalensis P.) mandarin seeds, of (Citrus reticulate B.); considered as new bioactive compounds. They were used, as dust application, to control cotton leafworm, S.littorais (Boisd.).The biological studies and influence of this natural compounds on the growth and development of 2 nd and 4 th instar larvae were investigated. The lethal time of larval mortality recorded after 24h, 48h, 72h, 7days and 2 weeks after larval treatment with seed powders. All treatments caused higher mortality in the 2 nd than in the 4 th instar larvae, compared with zero% mortality in the control. Larval growth inhibited, and pupation drastically reduced to 72, 60, 58%, after treatment with date pits, custard apple and mandarin, respectively, compere with 98% in the control. Moreover, this new bio-pesticide had a latent effect, until the emergence of adult moths, with many new scores of malformations. Samples of the treated larvae were electrophoretically examined and the total body proteins of the 4 th instar larval, treated with these compounds, performed by SDS-PAGE. Control and different treatments were separated into 24 different bands according to their relative frequencies (Rf values), and molecular weights (MW). The strong efficacy of the treated compounds on protein gel electrophoresis led to detection of new bands, and disappearance of some bands in comparison to control sample. Therefore, we recommend the use of this natural materials, as dust application, for the control of insect pests on their hosts under agricultural fields conditions.
The cabbage looper (Trichoplusia ni) is a medium-sized moth in the family Noctuidae, a family com... more The cabbage looper (Trichoplusia ni) is a medium-sized moth in the family Noctuidae, a family commonly referred to as owlet moths. Its common name comes from its preferred host plants and distinctive crawling behavior. The cabbage looper eggs are generally yellow-white in color, dome-shaped, and patterned with ridges. They are 0.6mm in diameter, they are usually laid singly on leaves. In one day, 40-50 females can lay 1000-2000 viable eggs. Viable eggs hatch after about three days The first record is the hatching process time (hatching period) of fully developed embryo within the egg was firstly video recorded. The elapsed time interval from the start till the completion of hatching process was calculated for three video recorded shots for egg eclosions of the full mature embryos of the cabbage looper,ranged between 12:30 minutes with an average interval period amounted to 21 minutes after feeding on Cabbage plant leaves.
www.eajbs.eg.net Virulence of Entomopathogenic Nematodes Steinernema glaseri and Heterorhabditis ... more www.eajbs.eg.net Virulence of Entomopathogenic Nematodes Steinernema glaseri and Heterorhabditis bacteriophora Poinar (HP 88 strain) Against the Black Cutworm, Agrotis ipsilon.
Journal of Plant Protection and Pathology
The objective of the current study was to identification and evaluate the essential oils "Eos." o... more The objective of the current study was to identification and evaluate the essential oils "Eos." of cotton and tomato plants to determine the responsive attraction of some pests; Spodoptera littoralis (Boisd.), Pectinophora gossypilla (Sound.) and Earias insulana (Boisd.) which caused a lot of damage to cotton and vegetable crops in Egypt. An experiment of two choice olfactometer systems was used in moths' bioassay to study the effect of which part of cotton plant and three tomato varieties can attract or repellent of volatile oils EOs. Essential oils of cotton and tomato plants were extracted and chemically identified by Gas Chromatography/Mass Spectrometry (GC/MS). Eighty-six volatile compounds were identified from leaves, bolls and flowers of cotton representing (99.45, 99.57 and 99.02%) from total mass, respectively. The major constituent of their chemical composition was Caryophyllene (17.83, 22.01 and 24.63 % for cotton leaves, flowers and bolls, respectively), D-Limonene recorded the largest compound in tomato varieties ("Real Madrid" 20.35 %, "Bs" 10.49 % and "Alissa" 12.07 %). Laboratory bioassay of the target pest "female and male moths" of P. gossypilla, obtained that the highest total response were83.09, 64.0 and 57.0 % for cotton leaves, flowers and bolls oil. E. insulana was the highest attracted moths to cotton bolls (64.91 %.), while S. littoralis estimated a positive response to cotton leaves (76.6 %). The present results may providea new strategy in the future to use plant essential oils as amixture baited on pheromone traps to attract and kill those pests.
J. Plant Prot. and Path., Mansoura Univ.,, 2017
Two species of the Entomopathogenic nematodes, Steinernema carpocapsae and Steinernema scapterisi... more Two species of the Entomopathogenic nematodes, Steinernema carpocapsae and Steinernema scapterisici were studied against cotton leaf worm Spodoptera littoralis (Boisd) and black cutworm Agrotis ipsilon (Hufn.).Under laboratory conditions, by using different nematodes strains at Lc 50 values were 3, 6, 7 juveniles IJ/ml of S. scapterisici , S.carpocapsae , combination of both strains and using water for control .The results investigated that effect of all treatments on some biological aspects of target pests such as, larval duration of S. littoralis were recorded that, 11.5, 11.5,11 and 11.8 days, after treatments with S.carpocapsae , S. scapterisici, combination and control, respectively. Furthermore A.ipsilon showed that highly tolerance more than cotton leaf worm,also the percentages of larval mortality were 32 % for S.carpocapsae ,44 % for S. scapterisici, 26% for combination of both strains and no larvae mortality on control treatments. Also there is no significant differences between pupal duration of both target pests while, the results indicated that, significant differences between pupal weight (mg) of S. littoralis and A. ipsilon. No emergency of S.littoralis moths after treatment with strain S.carpocapsae and this percentage increased to 66 % with S. scapterisici, however the combination of both strains affected the emergency of moths to reach 33% compared with control 84%. As regard the 2 nd insect pest A.ipsilon showed that highly emergency of moths 92 % for control treatment with water only and 50 % when treated with S. scapterisici and combination of both strains while decreased to 33% for S.carpocapsae. Subsequently, all the biological aspects of adults of both pests as ovi-position, fecundity (Eggs/ female), fertility and also the intermediated shapes were affected mostly by S. scapterisici, these findings might be taken into consideration in evaluating the success of the Entomopathogenic nematodes as a pathogenic insecticides for S.littoralis and A. ipsilon as an economic pests of cotton plants and opens new approaches to develop highly efficient combined biological products on the basis of entomopathogenic nematodes as a bio-agents.
Nanoparticle compounds such as Moringa oleifera Lam. leaf powder, boiled white and brown eggshell... more Nanoparticle compounds such as Moringa oleifera Lam. leaf powder, boiled white and brown eggshells powder for chickens and kapritia spring water form Siwa Oasis in Egypt, these natural materials were evaluated for first time under laboratory conditions against the 4 th instar larvae of cotton leafworm Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae). The results investigated that new nanoparticles of all eggshells and M. oleifera powder and kapritia water affected on biological aspects of target pest such as, larval mortality were recorded that,10, 8, 6 and 4% for M. oleifera, kapritia water, brown and white eggshells, respectively, compared with control 1%, the intermediated shapes were recorded during this studied. Also adults moths emergency percentage of S.littoralis are decreased to 89 , 82, 73 and 65 % after treatment with white eggshells, brown eggshells , M. oleifera leaf powders and kapritia Siwa water, respectively, compared with control 93%. Subsequently, adults malformed increased after all treatments; fecundity (Eggs/ female), fertility and also were affected. These compounds considered as nanoparticle, these innovation technologies to enhance our country developmental strategies in order to achieved sustainable agriculture and integrated crop management by alternative insecticide, environmental protection form pollutant, increase natural enemies for insect and food safety for better human healthy for a new generation. Introduction The Egyptian cotton leafworm Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae) is the most dangerous pests in Egypt and Africa, causing significant economic loss of cotton, tomato, lettuce, strawberry and other vegetables crop in both greenhouses and open fields (Abd El-Razik and Mostafa, 2013 and EPPO, 2014). Moringa oleifera Lam. is medicinal plant with many pharmacological properties, leaves are a nutrient-dense food with high concentrations of protein, carbohydrates, fiber and a good source of several vitamins and minerals such as vitamins A, B, C, D, and E; folic acid; nicotinic acid; iron; calcium; zinc; potassium; magnesium and copper (Singh and Singh, 2019 and Walia et al., (2019). The chemical constituents of M.oleifera stems, leaves, flowers, pods and seeds are alkaloids, phenolicacids, gallic,
Dried fruit seeds; i.e. date pits powder of date palm trees (Phoenix dactylifera L.) swia variety... more Dried fruit seeds; i.e. date pits powder of date palm trees (Phoenix dactylifera L.) swia variety, wild custard apple seeds of (Annona senegalensis P.) mandarin seeds, of (Citrus reticulate B.); considered as new bioactive compounds. They were used, as dust application, to control cotton leafworm, S.littorais (Boisd.).The biological studies and influence of this natural compounds on the growth and development of 2 nd and 4 th instar larvae were investigated. The lethal time of larval mortality recorded after 24h, 48h, 72h, 7days and 2 weeks after larval treatment with seed powders. All treatments caused higher mortality in the 2 nd than in the 4 th instar larvae, compared with zero% mortality in the control. Larval growth inhibited, and pupation drastically reduced to 72, 60, 58%, after treatment with date pits, custard apple and mandarin, respectively, compere with 98% in the control. Moreover, this new bio-pesticide had a latent effect, until the emergence of adult moths, with many new scores of malformations. Samples of the treated larvae were electrophoretically examined and the total body proteins of the 4 th instar larval, treated with these compounds, performed by SDS-PAGE. Control and different treatments were separated into 24 different bands according to their relative frequencies (Rf values), and molecular weights (MW). The strong efficacy of the treated compounds on protein gel electrophoresis led to detection of new bands, and disappearance of some bands in comparison to control sample. Therefore, we recommend the use of this natural materials, as dust application, for the control of insect pests on their hosts under agricultural fields conditions.
The cabbage looper (Trichoplusia ni) is a medium-sized moth in the family Noctuidae, a family com... more The cabbage looper (Trichoplusia ni) is a medium-sized moth in the family Noctuidae, a family commonly referred to as owlet moths. Its common name comes from its preferred host plants and distinctive crawling behavior. The cabbage looper eggs are generally yellow-white in color, dome-shaped, and patterned with ridges. They are 0.6mm in diameter, they are usually laid singly on leaves. In one day, 40-50 females can lay 1000-2000 viable eggs. Viable eggs hatch after about three days The first record is the hatching process time (hatching period) of fully developed embryo within the egg was firstly video recorded. The elapsed time interval from the start till the completion of hatching process was calculated for three video recorded shots for egg eclosions of the full mature embryos of the cabbage looper,ranged between 12:30 minutes with an average interval period amounted to 21 minutes after feeding on Cabbage plant leaves.
www.eajbs.eg.net Virulence of Entomopathogenic Nematodes Steinernema glaseri and Heterorhabditis ... more www.eajbs.eg.net Virulence of Entomopathogenic Nematodes Steinernema glaseri and Heterorhabditis bacteriophora Poinar (HP 88 strain) Against the Black Cutworm, Agrotis ipsilon.
Journal of Plant Protection and Pathology
The objective of the current study was to identification and evaluate the essential oils "Eos." o... more The objective of the current study was to identification and evaluate the essential oils "Eos." of cotton and tomato plants to determine the responsive attraction of some pests; Spodoptera littoralis (Boisd.), Pectinophora gossypilla (Sound.) and Earias insulana (Boisd.) which caused a lot of damage to cotton and vegetable crops in Egypt. An experiment of two choice olfactometer systems was used in moths' bioassay to study the effect of which part of cotton plant and three tomato varieties can attract or repellent of volatile oils EOs. Essential oils of cotton and tomato plants were extracted and chemically identified by Gas Chromatography/Mass Spectrometry (GC/MS). Eighty-six volatile compounds were identified from leaves, bolls and flowers of cotton representing (99.45, 99.57 and 99.02%) from total mass, respectively. The major constituent of their chemical composition was Caryophyllene (17.83, 22.01 and 24.63 % for cotton leaves, flowers and bolls, respectively), D-Limonene recorded the largest compound in tomato varieties ("Real Madrid" 20.35 %, "Bs" 10.49 % and "Alissa" 12.07 %). Laboratory bioassay of the target pest "female and male moths" of P. gossypilla, obtained that the highest total response were83.09, 64.0 and 57.0 % for cotton leaves, flowers and bolls oil. E. insulana was the highest attracted moths to cotton bolls (64.91 %.), while S. littoralis estimated a positive response to cotton leaves (76.6 %). The present results may providea new strategy in the future to use plant essential oils as amixture baited on pheromone traps to attract and kill those pests.
J. Plant Prot. and Path., Mansoura Univ.,, 2017
Two species of the Entomopathogenic nematodes, Steinernema carpocapsae and Steinernema scapterisi... more Two species of the Entomopathogenic nematodes, Steinernema carpocapsae and Steinernema scapterisici were studied against cotton leaf worm Spodoptera littoralis (Boisd) and black cutworm Agrotis ipsilon (Hufn.).Under laboratory conditions, by using different nematodes strains at Lc 50 values were 3, 6, 7 juveniles IJ/ml of S. scapterisici , S.carpocapsae , combination of both strains and using water for control .The results investigated that effect of all treatments on some biological aspects of target pests such as, larval duration of S. littoralis were recorded that, 11.5, 11.5,11 and 11.8 days, after treatments with S.carpocapsae , S. scapterisici, combination and control, respectively. Furthermore A.ipsilon showed that highly tolerance more than cotton leaf worm,also the percentages of larval mortality were 32 % for S.carpocapsae ,44 % for S. scapterisici, 26% for combination of both strains and no larvae mortality on control treatments. Also there is no significant differences between pupal duration of both target pests while, the results indicated that, significant differences between pupal weight (mg) of S. littoralis and A. ipsilon. No emergency of S.littoralis moths after treatment with strain S.carpocapsae and this percentage increased to 66 % with S. scapterisici, however the combination of both strains affected the emergency of moths to reach 33% compared with control 84%. As regard the 2 nd insect pest A.ipsilon showed that highly emergency of moths 92 % for control treatment with water only and 50 % when treated with S. scapterisici and combination of both strains while decreased to 33% for S.carpocapsae. Subsequently, all the biological aspects of adults of both pests as ovi-position, fecundity (Eggs/ female), fertility and also the intermediated shapes were affected mostly by S. scapterisici, these findings might be taken into consideration in evaluating the success of the Entomopathogenic nematodes as a pathogenic insecticides for S.littoralis and A. ipsilon as an economic pests of cotton plants and opens new approaches to develop highly efficient combined biological products on the basis of entomopathogenic nematodes as a bio-agents.