Sarfaraz Hadi - Academia.edu (original) (raw)
Papers by Sarfaraz Hadi
Acta horticulturae, Nov 1, 2006
Previously for micropropagation of date palm only shoot tip was used as an explant. In an attempt... more Previously for micropropagation of date palm only shoot tip was used as an explant. In an attempt to utilize different explants, shoot tip and base of flower rachis of male and female plants of variety 'Barhy' were used to initiate callus cultures on modified Murashige and Skoog medium containing different concentrations of 2,4dichlorophenoxyacetic acid (2,4-D), 2-isopentenylaminpurine (2-iP), kinetin and αnaphthalene acetic acid (NAA). Male shoot tip cultures produced embryogenic callus at significantly lower frequency as compared to the female cultures. Female rachis cultures produced embryogenic callus while male rachis cultures produced mostly non-embryogenic callus. After two years, only female shoot tip cultures produced plantlets. Liberation of phenolics was significantly higher in male cultures, enabling differentiation between male and female plants and justifying the slow growth of male cultures. For early identification of cultivars and tracing genetic diversity among date palm genotypes of different origin, offshoot-derived, male and female plants of cultivars 'Barhy' and 'Sukkary', seed-derived plants, and two in vitro cultures of both of these cultivars were subjected to Randomly Amplified Polymorphic DNA (RAPD) analysis. Similarity matrixes based on Nei and Li's coefficients show that offshoot-derived male plant of 'Barhy' was 73.6% genetically similar to its female counterpart, while similarity between male and female plants of 'Sukkary' was 43.1%. In the case of seedlings, male and female plants of 'Barhy' were 87.2% similar and those of 'Sukkary' were 62.3% genetically alike. Two in vitro cultures of 'Barhy' and Sukkary were 73.3% and 70% similar to the normal offshoot-derived plant respectively. These affinities were also reflected in cluster analysis by unweighted paired group means (UPGMA). Results of the study suggested that DNA fingerprinting can be utilized for early detection of date palm sex and genotype and that in vitro behavior of male and female plants of date palm cultivars is differential and their DNA profiles are divergent, which can help in selecting preferable males.
African Journal of Microbiology Research, 2011
Fungi, isolated from sea water, were subjected to growth in a medium containing low density polye... more Fungi, isolated from sea water, were subjected to growth in a medium containing low density polyethylene (LDPE) as the sole carbon source with and without yeast extract. Increasing fresh weight of the fungi in the medium supplemented with LDPE after regular time intervals gave the evidence that the fungi were utilizing LDPE as the carbon source. Further confirmation of LDPE utilization was carried out by the Sturm test where the degradation was attributed to the amount of carbon dioxide evolved during the growth period. The two fungi that showed good growth in medium supplemented with LDPE proved to degrade LDPE with higher efficiency in earlier reported results Scanning Electron Microscopy analysis of the fungal treated LDPE films provided a solid evidence of biodegradation. Fungi were identified as Aspergillus spp. LDPE degradation is a severe environmental crisis in the world and we have proved that microorganisms can be used for bioremediation in this line.
Saudi Journal of Biological Sciences, 2017
Sporosarcina pasteurii, a common soil bacterium has been tested for microbial treatment of cement... more Sporosarcina pasteurii, a common soil bacterium has been tested for microbial treatment of cement mortar. The present study also seeks to investigate the effects of growth medium, bacterial concentration and different buffers concerning the preparation of bacterial suspensions on the compressive strength of cement mortar. Two growth media, six different suspensions and two bacterial concentrations were used in the study. The influence of growth medium on calcification efficiency of S. pasteurii was insignificant. Significant improvement in the compressive as well as the tensile strength of cement mortar was observed. Microbial mineral precipitation visualized by Scanning Electron Microscopy (SEM) shows fibrous material that increased the strength of cement mortar. Formation of thin strands of fillers observed through SEM micrographs improves the pore structure, impermeability and thus the compressive as well as the tensile strengths of the cement mortar. The type of substrate and its molarity have a significant influence on the strength of cement mortar.
Journal of Materials Research and Technology
We consider a class S of stochastic processes X := {X (t)} t∈[0,T ] whose realizations x := x (t)... more We consider a class S of stochastic processes X := {X (t)} t∈[0,T ] whose realizations x := x (t) (t ∈ [0, T ]) are real continuous piecewise linear functions satisfying a particular geometric condition. Let R be the family of all binary responses Y, Y ∈ {bad, good}, associated to a process X in S. Basing on data arising from a continuous phenomenon which can be simulated by a couple (X, Y) ∈ S × R, we introduce the notion of adjustment curve for the binary response Y of the process X, that is a decreasing function γ a : [0, T ] → [0, 1] which gives the probability that a new realization x of X is adjustable at the time t ∈ [0, T ]. For real industrial processes, which can be modelized by (X, Y) ∈ S × R, our model can be used for monitoring and predicting the quality of the product.
'Shees' fruit abnormality manifested as cluster of small fruit-like structures on a single pedunc... more 'Shees' fruit abnormality manifested as cluster of small fruit-like structures on a single peduncle occurs at a high frequency in tissue culture derived plants of date palm. The abnormality is akin to parthenocarpy, which is known to be caused by altered hormone profile of flowers/fruits in many species. This study was conducted to elucidate the hormonal profile of date palm flowers and early fruits and to identify the hormones associated with 'shees' fruit formation. Hormone levels in young flowers/fruits of normal and 'shees'-bearing plants of cultivars 'Barhy' and 'Nabtet-Saif' were studied with HPLC at the time of pollination and subsequently after 10 and 20 days. In all these samples, out of seven hormones detected by HPLC, levels of indole-3-acetic acid (IAA) and gebberellic acid (GA 3) were significantly higher, while that of a 'kinetin-like' compound and an unknown compound were significantly lower in 'shees' flowers/fruits as compared to the normal counterparts. Other three unknown compounds did not show significant variations between normal and 'shees' fruits. Kinetin-like compound showed the same elution properties as kinetin standard during HPLC procedures. The compound was purified and an attempt was made to characterize the molecule with gas chromatography-mass spectrometry (GC-MS). On the basis of Wiley-229 library hit (entry 101383) the compound was identified as Benzeldehyde, 2-hydroxy-[(2-hydroxyphenyl) methylene] hydrazone with formula: C 14 H 12 N 2 O 2 and Mol weight: 240. We have tentatively designated the compound as BHH and are reporting the occurrence of this compound for the first time in date palm.
To avoid the harmful effects of the chemical fungicides on the human and minimize the environment... more To avoid the harmful effects of the chemical fungicides on the human and minimize the environmental pollution, an alternative ecofriendly control strategies should be developed. The extract of Commiphora myhrra L. was tested against twenty fungal genera isolated from the indoor air collected from different rooms in King Saud University, kingdom of Saudi Arabia. Disc diffusion test was modified for use in this study and the collected data was statistically analyzed. Variable antifungal efficacy of different myrrh extract was recorded against the investigated fungal genera. The efficacy of the extract was increased as the concentration increased. The highest growth inhibition (74.6%) was against Acremonium strictum followed by Trichoderma psuedokoningii (70.6%). On contrast, the lowest efficacy (12.7%) was against Ulocladium consortiale. It could be concluded that myrrh extract is promised as a source of substances from which of safer and ecofriendly could be used as antimicrobial age...
Exudate of an Arabian shrub 'Myrrh' (Commiphora myrrha L.) has been traditionally used fo... more Exudate of an Arabian shrub 'Myrrh' (Commiphora myrrha L.) has been traditionally used for curing a wide range of ailments in Arab-African region. This study attempted assessing in-vitro efficacy of myrrh against airborne fungi and some bacteria. Extract of myrrh in methanol, ethanol, boiling water, and normal was tested under in-vitro conditions against 20 airborne fungi and 10 pathogenic bacteria. Extracts in methanol and ethanol showed greater efficacy against fungi as compared to extracts in boiling and normal water. Variable levels of efficacy were recorded against different fungi probably due to genotypic specificity to diverse molecules in different extracts. Optimal concentration of myrrh was found to be 15% (w/v). Interestingly, myrrh extracts showed no efficacy against bacteria. GC-MS analysis of myrrh extracts in alcohols revealed a total of 14 molecules, 11 of which are being reported for the first time. It is expected that this study will benefit students and re...
Construction and Building Materials
Abstract Cementitious composites are widely used in the construction industry around the globe. N... more Abstract Cementitious composites are widely used in the construction industry around the globe. Nevertheless, these are prone to cracking because of their low tensile strength. The reinforcement provided to carry tensile stresses gets exposed to the outside environment due to cracking thereby reducing the service life of structures. The control of crack widths in concrete is thus necessary to ensure durability. Research studies have shown that bacteria exist that are able to precipitate calcite, and may thus heal cracks thereby improving the durability of concrete. This technique is highly desirable because the microbially induced calcite precipitation (MICP) is pollution-free and natural. This novel technique may also be used in protecting historical stone monuments without affecting their appearance. This article reviews the process of bioremediation of cementitious composites employed for improving their properties. Different parameters affecting the process of MICP such as the bacterial genotypes, biotic, and abiotic factors are elucidated. Methods employed by various researchers for improving strength, durability, and crack remediation are reviewed. The tests employed for the assessment of the success of MICP are thoroughly investigated. This paper presents an exhaustive review by covering most of the studies available in published literature till date. The scope of research for closing the gaps between research and practice for different fields of applications have been identified. In the end, a summary of the research status of bio-induction and bioremediation of cementitious composites is given which highlights its great potential and need for further research.
XXVII International Horticultural Congress - IHC2006: International Symposium on Structural and Functional Genomics of Horticultural Plants, 2007
... 2). All the selected primers were among the 37 prescreened primers for date palm DNA amplific... more ... 2). All the selected primers were among the 37 prescreened primers for date palm DNA amplification ... UPGMA) also showed close clustering of the offshoot-derived plants indicating theirtrue-to-type ... Data in the similarity matrix and the phylo-genetic tree indicated that TC plants ...
IV International Date Palm Conference, 2010
Twenty eight fungal isolates belonging to 12 genera were derived from debris, sediment and water ... more Twenty eight fungal isolates belonging to 12 genera were derived from debris, sediment and water samples collected from Avicennia marina stands 25km south of Jeddah city on the Red Sea coast of Saudi Arabia. Eight of these isolates were found to be able to grow in association cellulosic waste materials under in vitro conditions in the absence of any carbon source. Isolates were further tested for their potential to degrade paper and clothes wastes by co-cultivation under aeration on a rotary shaker. These fungi accumulated significantly higher biomass, produced ligninolytic and cellulase enzymes, and liberated larger volumes of CO2. These observations indicated that the selected isolates were able to breakdown and consume the waste materials.
Influence of sucrose concentration of the medium, genotype and interaction of the two on in vitro... more Influence of sucrose concentration of the medium, genotype and interaction of the two on in vitro growth responses of the cultures of five hybrid-tea rose (Rosa hybrida L.) cultivars were evident from the initiation of shoot proliferation from axillary buds on MS medium containing 4.0 mg/l BA, 0.2 mg/l Kn 1.0 mg/l GA3 and 30 g/l sucrose. Emerging shoots were subcultured to media containing 3.0 mg/l BA, 0.2 mg/l Kn and three different concentrations of sucrose (30, 40 and 50 g/l). Number of nascent shoots produced and elongation of the main shoot in a four-week-period was recorded for three consecutive subcultures in each concen-tration of sucrose. Rooting was induced on a medium containing 1.0 mg/l of IBA and respective sucrose levels. Sucrose concentration of 40 g/l produced the best response followed by 30 and 50 g/l. Genotypic influence was also apparent on growth responses with significant differences between values for shoots produced and shoot elongation in different cultivars...
V International Symposium …, 2004
... A date palm tree produces limited number of offshoots in its life span. ... The phenomenon ha... more ... A date palm tree produces limited number of offshoots in its life span. ... The phenomenon has been reported in date palm also (Al-Wasel, 2001; Azeqour et al., 2002), this is considered to be a disadvantages due to the minimization of true to type plants . ...
Saudi journal of biological sciences, 2018
Blue tilapia, , was experimentally infected with , a bacterium that damages the gills, liver, and... more Blue tilapia, , was experimentally infected with , a bacterium that damages the gills, liver, and intestine, resulting in histopathological changes in the infected organs. Our histopathological study showed an aggregation of hemocytes with cell necrosis in gills; a massive aggregation of hemocytes and pyknotic nuclei in the hepatopancreas; and a lower rate of hemocyte aggregation in the digestive system of the infected fish.
The Journal of General and Applied Microbiology, 2015
The pollution of land and water by petroleum compounds is a matter of growing concern necessitati... more The pollution of land and water by petroleum compounds is a matter of growing concern necessitating the development of methodologies, including microbial biodegradation, to minimize the impending impacts. It has been extensively reported that fungi from polluted habitats have the potential to degrade pollutants, including petroleum compounds. The Red Sea is used extensively for the transport of oil and is substantially polluted, due to leaks, spills, and occasional accidents. Tidal water, floating debris, and soil sediment were collected from mangrove stands on three polluted sites along the Red Sea coast of Saudi Arabia and forty-five fungal isolates belonging to 13 genera were recovered from these samples. The isolates were identified on the basis of a sequence analysis of the 18S rRNA gene fragment. Nine of these isolates were found to be able to grow in association with engine oil, as the sole carbon source, under in vitro conditions. These selected isolates and their consortium accumulated greater biomass, liberated more CO 2 , and produced higher levels of extracellular enzymes, during cultivation with engine oil as compared with the controls. These observations were authenticated by gas chromatography-mass spectrophotometry (GC-MS) analysis, which indicated that many high mass compounds present in the oil before treatment either disappeared or showed diminished levels.
Journal of Pure and Applied Microbiology
The study was aimed at increasing the production of polygalacturonase (PG) in Saccharomyces cerev... more The study was aimed at increasing the production of polygalacturonase (PG) in Saccharomyces cerevisiae by the process of mutation. A high PG-secreting strain of S. crerevisiae was exposed to different doses of diethyl sulfate (DES) and surviving colonies were screened for PG activity by plate assay method. Survival rate and mutation frequency were recorded under different treatments. Many selections obtained in the study showed PG activity in excess of the starting strain and the wild type reference strain. PCR analysis of the mutants revealed unique markers in some of the selections which corroborated well with their respective PG activity. The study augments the relevance of mutagenesis for strain improvement in fungi for production of useful industrial enzymes.
Saudi Journal of Biological Sciences, 2015
Mangrove sediments were collected from major mangrove stands on the Red Sea Coast of Saudi Arabia... more Mangrove sediments were collected from major mangrove stands on the Red Sea Coast of Saudi Arabia. Forty five isolates belonging to 12 genera were purified and five isolates as well as their consortium were found to be able to grow in association with petroleum oil as sole carbon source under in vitro conditions. The isolated strains were identified based on internal transcribed spacer (ITS) rDNA sequence analysis. The fungal strains with the greatest potentiality to degrade diesel oil, without developing antagonistic activity, were identified as Alternaria alternata, Aspergillus terreus, Cladosporium sphaerospermum, Eupenicillium hirayamae and Paecilomyces variotii. As compared to the controls, these fungi accumulated significantly higher biomass, produced extracellular enzymes and liberated larger volumes of CO 2. These observations with GC-MS data confirm that these isolates displayed rapid diesel oil bioremoval and when used together as a consortium, there was no antagonistic activity.
Saudi Journal of Biological Sciences, 2015
Mangrove sediments were collected from major mangrove stands on the Red Sea Coast of Saudi Arabia... more Mangrove sediments were collected from major mangrove stands on the Red Sea Coast of Saudi Arabia. Forty five isolates belonging to 12 genera were purified and five isolates as well as their consortium were found to be able to grow in association with petroleum oil as sole carbon source under in vitro conditions. The isolated strains were identified based on internal transcribed spacer (ITS) rDNA sequence analysis. The fungal strains with the greatest potentiality to degrade diesel oil, without developing antagonistic activity, were identified as Alternaria alternata, Aspergillus terreus, Cladosporium sphaerospermum, Eupenicillium hirayamae and Paecilomyces variotii. As compared to the controls, these fungi accumulated significantly higher biomass, produced extracellular enzymes and liberated larger volumes of CO 2. These observations with GC-MS data confirm that these isolates displayed rapid diesel oil bioremoval and when used together as a consortium, there was no antagonistic activity.
Acta horticulturae, Nov 1, 2006
Previously for micropropagation of date palm only shoot tip was used as an explant. In an attempt... more Previously for micropropagation of date palm only shoot tip was used as an explant. In an attempt to utilize different explants, shoot tip and base of flower rachis of male and female plants of variety 'Barhy' were used to initiate callus cultures on modified Murashige and Skoog medium containing different concentrations of 2,4dichlorophenoxyacetic acid (2,4-D), 2-isopentenylaminpurine (2-iP), kinetin and αnaphthalene acetic acid (NAA). Male shoot tip cultures produced embryogenic callus at significantly lower frequency as compared to the female cultures. Female rachis cultures produced embryogenic callus while male rachis cultures produced mostly non-embryogenic callus. After two years, only female shoot tip cultures produced plantlets. Liberation of phenolics was significantly higher in male cultures, enabling differentiation between male and female plants and justifying the slow growth of male cultures. For early identification of cultivars and tracing genetic diversity among date palm genotypes of different origin, offshoot-derived, male and female plants of cultivars 'Barhy' and 'Sukkary', seed-derived plants, and two in vitro cultures of both of these cultivars were subjected to Randomly Amplified Polymorphic DNA (RAPD) analysis. Similarity matrixes based on Nei and Li's coefficients show that offshoot-derived male plant of 'Barhy' was 73.6% genetically similar to its female counterpart, while similarity between male and female plants of 'Sukkary' was 43.1%. In the case of seedlings, male and female plants of 'Barhy' were 87.2% similar and those of 'Sukkary' were 62.3% genetically alike. Two in vitro cultures of 'Barhy' and Sukkary were 73.3% and 70% similar to the normal offshoot-derived plant respectively. These affinities were also reflected in cluster analysis by unweighted paired group means (UPGMA). Results of the study suggested that DNA fingerprinting can be utilized for early detection of date palm sex and genotype and that in vitro behavior of male and female plants of date palm cultivars is differential and their DNA profiles are divergent, which can help in selecting preferable males.
African Journal of Microbiology Research, 2011
Fungi, isolated from sea water, were subjected to growth in a medium containing low density polye... more Fungi, isolated from sea water, were subjected to growth in a medium containing low density polyethylene (LDPE) as the sole carbon source with and without yeast extract. Increasing fresh weight of the fungi in the medium supplemented with LDPE after regular time intervals gave the evidence that the fungi were utilizing LDPE as the carbon source. Further confirmation of LDPE utilization was carried out by the Sturm test where the degradation was attributed to the amount of carbon dioxide evolved during the growth period. The two fungi that showed good growth in medium supplemented with LDPE proved to degrade LDPE with higher efficiency in earlier reported results Scanning Electron Microscopy analysis of the fungal treated LDPE films provided a solid evidence of biodegradation. Fungi were identified as Aspergillus spp. LDPE degradation is a severe environmental crisis in the world and we have proved that microorganisms can be used for bioremediation in this line.
Saudi Journal of Biological Sciences, 2017
Sporosarcina pasteurii, a common soil bacterium has been tested for microbial treatment of cement... more Sporosarcina pasteurii, a common soil bacterium has been tested for microbial treatment of cement mortar. The present study also seeks to investigate the effects of growth medium, bacterial concentration and different buffers concerning the preparation of bacterial suspensions on the compressive strength of cement mortar. Two growth media, six different suspensions and two bacterial concentrations were used in the study. The influence of growth medium on calcification efficiency of S. pasteurii was insignificant. Significant improvement in the compressive as well as the tensile strength of cement mortar was observed. Microbial mineral precipitation visualized by Scanning Electron Microscopy (SEM) shows fibrous material that increased the strength of cement mortar. Formation of thin strands of fillers observed through SEM micrographs improves the pore structure, impermeability and thus the compressive as well as the tensile strengths of the cement mortar. The type of substrate and its molarity have a significant influence on the strength of cement mortar.
Journal of Materials Research and Technology
We consider a class S of stochastic processes X := {X (t)} t∈[0,T ] whose realizations x := x (t)... more We consider a class S of stochastic processes X := {X (t)} t∈[0,T ] whose realizations x := x (t) (t ∈ [0, T ]) are real continuous piecewise linear functions satisfying a particular geometric condition. Let R be the family of all binary responses Y, Y ∈ {bad, good}, associated to a process X in S. Basing on data arising from a continuous phenomenon which can be simulated by a couple (X, Y) ∈ S × R, we introduce the notion of adjustment curve for the binary response Y of the process X, that is a decreasing function γ a : [0, T ] → [0, 1] which gives the probability that a new realization x of X is adjustable at the time t ∈ [0, T ]. For real industrial processes, which can be modelized by (X, Y) ∈ S × R, our model can be used for monitoring and predicting the quality of the product.
'Shees' fruit abnormality manifested as cluster of small fruit-like structures on a single pedunc... more 'Shees' fruit abnormality manifested as cluster of small fruit-like structures on a single peduncle occurs at a high frequency in tissue culture derived plants of date palm. The abnormality is akin to parthenocarpy, which is known to be caused by altered hormone profile of flowers/fruits in many species. This study was conducted to elucidate the hormonal profile of date palm flowers and early fruits and to identify the hormones associated with 'shees' fruit formation. Hormone levels in young flowers/fruits of normal and 'shees'-bearing plants of cultivars 'Barhy' and 'Nabtet-Saif' were studied with HPLC at the time of pollination and subsequently after 10 and 20 days. In all these samples, out of seven hormones detected by HPLC, levels of indole-3-acetic acid (IAA) and gebberellic acid (GA 3) were significantly higher, while that of a 'kinetin-like' compound and an unknown compound were significantly lower in 'shees' flowers/fruits as compared to the normal counterparts. Other three unknown compounds did not show significant variations between normal and 'shees' fruits. Kinetin-like compound showed the same elution properties as kinetin standard during HPLC procedures. The compound was purified and an attempt was made to characterize the molecule with gas chromatography-mass spectrometry (GC-MS). On the basis of Wiley-229 library hit (entry 101383) the compound was identified as Benzeldehyde, 2-hydroxy-[(2-hydroxyphenyl) methylene] hydrazone with formula: C 14 H 12 N 2 O 2 and Mol weight: 240. We have tentatively designated the compound as BHH and are reporting the occurrence of this compound for the first time in date palm.
To avoid the harmful effects of the chemical fungicides on the human and minimize the environment... more To avoid the harmful effects of the chemical fungicides on the human and minimize the environmental pollution, an alternative ecofriendly control strategies should be developed. The extract of Commiphora myhrra L. was tested against twenty fungal genera isolated from the indoor air collected from different rooms in King Saud University, kingdom of Saudi Arabia. Disc diffusion test was modified for use in this study and the collected data was statistically analyzed. Variable antifungal efficacy of different myrrh extract was recorded against the investigated fungal genera. The efficacy of the extract was increased as the concentration increased. The highest growth inhibition (74.6%) was against Acremonium strictum followed by Trichoderma psuedokoningii (70.6%). On contrast, the lowest efficacy (12.7%) was against Ulocladium consortiale. It could be concluded that myrrh extract is promised as a source of substances from which of safer and ecofriendly could be used as antimicrobial age...
Exudate of an Arabian shrub 'Myrrh' (Commiphora myrrha L.) has been traditionally used fo... more Exudate of an Arabian shrub 'Myrrh' (Commiphora myrrha L.) has been traditionally used for curing a wide range of ailments in Arab-African region. This study attempted assessing in-vitro efficacy of myrrh against airborne fungi and some bacteria. Extract of myrrh in methanol, ethanol, boiling water, and normal was tested under in-vitro conditions against 20 airborne fungi and 10 pathogenic bacteria. Extracts in methanol and ethanol showed greater efficacy against fungi as compared to extracts in boiling and normal water. Variable levels of efficacy were recorded against different fungi probably due to genotypic specificity to diverse molecules in different extracts. Optimal concentration of myrrh was found to be 15% (w/v). Interestingly, myrrh extracts showed no efficacy against bacteria. GC-MS analysis of myrrh extracts in alcohols revealed a total of 14 molecules, 11 of which are being reported for the first time. It is expected that this study will benefit students and re...
Construction and Building Materials
Abstract Cementitious composites are widely used in the construction industry around the globe. N... more Abstract Cementitious composites are widely used in the construction industry around the globe. Nevertheless, these are prone to cracking because of their low tensile strength. The reinforcement provided to carry tensile stresses gets exposed to the outside environment due to cracking thereby reducing the service life of structures. The control of crack widths in concrete is thus necessary to ensure durability. Research studies have shown that bacteria exist that are able to precipitate calcite, and may thus heal cracks thereby improving the durability of concrete. This technique is highly desirable because the microbially induced calcite precipitation (MICP) is pollution-free and natural. This novel technique may also be used in protecting historical stone monuments without affecting their appearance. This article reviews the process of bioremediation of cementitious composites employed for improving their properties. Different parameters affecting the process of MICP such as the bacterial genotypes, biotic, and abiotic factors are elucidated. Methods employed by various researchers for improving strength, durability, and crack remediation are reviewed. The tests employed for the assessment of the success of MICP are thoroughly investigated. This paper presents an exhaustive review by covering most of the studies available in published literature till date. The scope of research for closing the gaps between research and practice for different fields of applications have been identified. In the end, a summary of the research status of bio-induction and bioremediation of cementitious composites is given which highlights its great potential and need for further research.
XXVII International Horticultural Congress - IHC2006: International Symposium on Structural and Functional Genomics of Horticultural Plants, 2007
... 2). All the selected primers were among the 37 prescreened primers for date palm DNA amplific... more ... 2). All the selected primers were among the 37 prescreened primers for date palm DNA amplification ... UPGMA) also showed close clustering of the offshoot-derived plants indicating theirtrue-to-type ... Data in the similarity matrix and the phylo-genetic tree indicated that TC plants ...
IV International Date Palm Conference, 2010
Twenty eight fungal isolates belonging to 12 genera were derived from debris, sediment and water ... more Twenty eight fungal isolates belonging to 12 genera were derived from debris, sediment and water samples collected from Avicennia marina stands 25km south of Jeddah city on the Red Sea coast of Saudi Arabia. Eight of these isolates were found to be able to grow in association cellulosic waste materials under in vitro conditions in the absence of any carbon source. Isolates were further tested for their potential to degrade paper and clothes wastes by co-cultivation under aeration on a rotary shaker. These fungi accumulated significantly higher biomass, produced ligninolytic and cellulase enzymes, and liberated larger volumes of CO2. These observations indicated that the selected isolates were able to breakdown and consume the waste materials.
Influence of sucrose concentration of the medium, genotype and interaction of the two on in vitro... more Influence of sucrose concentration of the medium, genotype and interaction of the two on in vitro growth responses of the cultures of five hybrid-tea rose (Rosa hybrida L.) cultivars were evident from the initiation of shoot proliferation from axillary buds on MS medium containing 4.0 mg/l BA, 0.2 mg/l Kn 1.0 mg/l GA3 and 30 g/l sucrose. Emerging shoots were subcultured to media containing 3.0 mg/l BA, 0.2 mg/l Kn and three different concentrations of sucrose (30, 40 and 50 g/l). Number of nascent shoots produced and elongation of the main shoot in a four-week-period was recorded for three consecutive subcultures in each concen-tration of sucrose. Rooting was induced on a medium containing 1.0 mg/l of IBA and respective sucrose levels. Sucrose concentration of 40 g/l produced the best response followed by 30 and 50 g/l. Genotypic influence was also apparent on growth responses with significant differences between values for shoots produced and shoot elongation in different cultivars...
V International Symposium …, 2004
... A date palm tree produces limited number of offshoots in its life span. ... The phenomenon ha... more ... A date palm tree produces limited number of offshoots in its life span. ... The phenomenon has been reported in date palm also (Al-Wasel, 2001; Azeqour et al., 2002), this is considered to be a disadvantages due to the minimization of true to type plants . ...
Saudi journal of biological sciences, 2018
Blue tilapia, , was experimentally infected with , a bacterium that damages the gills, liver, and... more Blue tilapia, , was experimentally infected with , a bacterium that damages the gills, liver, and intestine, resulting in histopathological changes in the infected organs. Our histopathological study showed an aggregation of hemocytes with cell necrosis in gills; a massive aggregation of hemocytes and pyknotic nuclei in the hepatopancreas; and a lower rate of hemocyte aggregation in the digestive system of the infected fish.
The Journal of General and Applied Microbiology, 2015
The pollution of land and water by petroleum compounds is a matter of growing concern necessitati... more The pollution of land and water by petroleum compounds is a matter of growing concern necessitating the development of methodologies, including microbial biodegradation, to minimize the impending impacts. It has been extensively reported that fungi from polluted habitats have the potential to degrade pollutants, including petroleum compounds. The Red Sea is used extensively for the transport of oil and is substantially polluted, due to leaks, spills, and occasional accidents. Tidal water, floating debris, and soil sediment were collected from mangrove stands on three polluted sites along the Red Sea coast of Saudi Arabia and forty-five fungal isolates belonging to 13 genera were recovered from these samples. The isolates were identified on the basis of a sequence analysis of the 18S rRNA gene fragment. Nine of these isolates were found to be able to grow in association with engine oil, as the sole carbon source, under in vitro conditions. These selected isolates and their consortium accumulated greater biomass, liberated more CO 2 , and produced higher levels of extracellular enzymes, during cultivation with engine oil as compared with the controls. These observations were authenticated by gas chromatography-mass spectrophotometry (GC-MS) analysis, which indicated that many high mass compounds present in the oil before treatment either disappeared or showed diminished levels.
Journal of Pure and Applied Microbiology
The study was aimed at increasing the production of polygalacturonase (PG) in Saccharomyces cerev... more The study was aimed at increasing the production of polygalacturonase (PG) in Saccharomyces cerevisiae by the process of mutation. A high PG-secreting strain of S. crerevisiae was exposed to different doses of diethyl sulfate (DES) and surviving colonies were screened for PG activity by plate assay method. Survival rate and mutation frequency were recorded under different treatments. Many selections obtained in the study showed PG activity in excess of the starting strain and the wild type reference strain. PCR analysis of the mutants revealed unique markers in some of the selections which corroborated well with their respective PG activity. The study augments the relevance of mutagenesis for strain improvement in fungi for production of useful industrial enzymes.
Saudi Journal of Biological Sciences, 2015
Mangrove sediments were collected from major mangrove stands on the Red Sea Coast of Saudi Arabia... more Mangrove sediments were collected from major mangrove stands on the Red Sea Coast of Saudi Arabia. Forty five isolates belonging to 12 genera were purified and five isolates as well as their consortium were found to be able to grow in association with petroleum oil as sole carbon source under in vitro conditions. The isolated strains were identified based on internal transcribed spacer (ITS) rDNA sequence analysis. The fungal strains with the greatest potentiality to degrade diesel oil, without developing antagonistic activity, were identified as Alternaria alternata, Aspergillus terreus, Cladosporium sphaerospermum, Eupenicillium hirayamae and Paecilomyces variotii. As compared to the controls, these fungi accumulated significantly higher biomass, produced extracellular enzymes and liberated larger volumes of CO 2. These observations with GC-MS data confirm that these isolates displayed rapid diesel oil bioremoval and when used together as a consortium, there was no antagonistic activity.
Saudi Journal of Biological Sciences, 2015
Mangrove sediments were collected from major mangrove stands on the Red Sea Coast of Saudi Arabia... more Mangrove sediments were collected from major mangrove stands on the Red Sea Coast of Saudi Arabia. Forty five isolates belonging to 12 genera were purified and five isolates as well as their consortium were found to be able to grow in association with petroleum oil as sole carbon source under in vitro conditions. The isolated strains were identified based on internal transcribed spacer (ITS) rDNA sequence analysis. The fungal strains with the greatest potentiality to degrade diesel oil, without developing antagonistic activity, were identified as Alternaria alternata, Aspergillus terreus, Cladosporium sphaerospermum, Eupenicillium hirayamae and Paecilomyces variotii. As compared to the controls, these fungi accumulated significantly higher biomass, produced extracellular enzymes and liberated larger volumes of CO 2. These observations with GC-MS data confirm that these isolates displayed rapid diesel oil bioremoval and when used together as a consortium, there was no antagonistic activity.